UBA3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBA3
Entrez Gene ID	9039
Full Name	ubiquitin like modifier activating enzyme 3
Synonyms	NAE2,UBE1C,hUBA3
General protein information	Preferred Names ubiquitin like modifier activating enzyme 3 Names NEDD8-activating enzyme E1 catalytic subunit NEDD8-activating enzyme E1 subunit 2 NEDD8-activating enzyme E1C Nedd8-activating enzyme hUba3 UBA3, ubiquitin-activating enzyme E1 homolog ubiquitin-activating enzyme 3 ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) ubiquitin-activating enzyme E1C (homologous to yeast UBA3)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p14.1
Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	603172

mRNA and Protein(s)

mRNA	Protein	Name
NM_003968.3	NP_003959.3	NEDD8-activating enzyme E1 catalytic subunit isoform 1
NM_198195.2	NP_937838.1	NEDD8-activating enzyme E1 catalytic subunit isoform 2
XM_011534210.1	XP_011532512.1	NEDD8-activating enzyme E1 catalytic subunit isoform X1
NM_198195.1	NP_937838.1	NEDD8-activating enzyme E1 catalytic subunit isoform 2
XM_011534211.1	XP_011532513.1	NEDD8-activating enzyme E1 catalytic subunit isoform X2
NM_001363861.1	NP_001350790.1	NEDD8-activating enzyme E1 catalytic subunit isoform 3
NM_003968.4	NP_003959.3	NEDD8-activating enzyme E1 catalytic subunit isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UBA3	NP_001069042.1
Caenorhabditis elegans (roundworm)	rfl-1	NP_498534.2
Canis lupus familiaris (dog)	UBA3	XP_864203.1
Magnaporthe oryzae (rice blast fungus)	MGG_09283	XP_003709928.1
Homo sapiens (human)	UBA3	NP_003959.3
Rattus norvegicus (Norway rat)	Uba3	NP_476553.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005102	XP_313978.1
Pan troglodytes (chimpanzee)	UBA3	XP_516573.2
	UBA3	XP_001088381.2
Mus musculus (house mouse)	Uba3	NP_035796.2
Danio rerio (zebrafish)	uba3	NP_998632.1
Xenopus tropicalis (tropical clawed frog)	uba3	NP_001116198.1
Gallus gallus (chicken)	UBA3	XP_004944740.1
Drosophila melanogaster (fruit fly)	Uba3	NP_610913.1
Schizosaccharomyces pombe (fission yeast)	uba3	NP_592940.1
Neurospora crassa	NCU02532	XP_965663.1
Arabidopsis thaliana (thale cress)	ECR1	NP_568370.1
Oryza sativa (rice)	Os01g0271500	NP_001042708.1

Gene Ontology
Bibliography

Related articles in PubMed

Neddylation pathway is up-regulated in human intrahepatic cholangiocarcinoma and serves as a potential therapeutic target.
Gao Q, Yu GY, Shi JY, Li LH, Zhang WJ, Wang ZC, Yang LX, Duan M, Zhao H, Wang XY, Zhou J, Qiu SJ, Jeong LS, Jia LJ, Fan J
Oncotarget5(17)7820-32(2014 Sep)

Suppression of tumor angiogenesis by targeting the protein neddylation pathway.
Yao WT, Wu JF, Yu GY, Wang R, Wang K, Li LH, Chen P, Jiang YN, Cheng H, Lee HW, Yu J, Qi H, Yu XJ, Wang P, Chu YW, Yang M, Hua ZC, Ying HQ, Hoffman RM, Jeong LS, Jia LJ
Cell death & disease5e1059(2014 Feb)

Mutations in UBA3 confer resistance to the NEDD8-activating enzyme inhibitor MLN4924 in human leukemic cells.
Xu GW, Toth JI, da Silva SR, Paiva SL, Lukkarila JL, Hurren R, Maclean N, Sukhai MA, Bhattacharjee RN, Goard CA, Medeiros B, Gunning PT, Dhe-Paganon S, Petroski MD, Schimmer AD
PloS one9(4)e93530(2014)

E2-binding surface on Uba3 ?-grasp domain undergoes a conformational transition.
Elgin ES, S?kmen N, Peterson FC, Volkman BF, Da? C, Haas AL
Proteins80(10)2482-7(2012 Oct)

Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network.
Markson G, Kiel C, Hyde R, Brown S, Charalabous P, Bremm A, Semple J, Woodsmith J, Duley S, Salehi-Ashtiani K, Vidal M, Komander D, Serrano L, Lehner P, Sanderson CM
Genome research19(10)1905-11(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

E1 (NAE1 and UBA3) and E2 (UBC12) enzymes, as well as global NEDD8 conjugation, were upregulated in over 2/3 of human intrahepatic cholangiocarcinoma
Title: Neddylation pathway is up-regulated in human intrahepatic cholangiocarcinoma and serves as a potential therapeutic target.

Report role for neddylation via Nedd8-activating enzyme in the regulation of tumor angiogenesis.
Title: Suppression of tumor angiogenesis by targeting the protein neddylation pathway.

The study demonstrated that two mutations in UBA3 which were not previously reported confer MLN4924 resistance to MLN4924, a selective NEDD8-activating enzyme inhibitor.
Title: Mutations in UBA3 confer resistance to the NEDD8-activating enzyme inhibitor MLN4924 in human leukemic cells.

Demonstrated that Uba3-betaGD is an independently folded domain in solution and that residues involved in E2 binding are absent from the NMR spectrum, indicating that the E2-binding surface on Uba3-betaGD interconverts between multiple conformations.
Title: E2-binding surface on Uba3 ?-grasp domain undergoes a conformational transition.

X-ray crystallographic analysis of APPBP1-UBA3-NEDD8 shows that APPBP1-UBA3's preference for NEDD8's Ala72 appears to be indirect, due to proper positioning of UBA3's Arg190.
Title: Structural dissection of a gating mechanism preventing misactivation of ubiquitin by NEDD8's E1.

The following UBA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03627	NM_003968.4 Latest version!	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu02511	NM_198195.2 Latest version!	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu62400	NM_001363861.1 Latest version!	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu62399	XM_011534210.1 Latest version!	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu62400	XM_011534211.1 Latest version!	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu02511	NM_198195.1	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu03627	NM_003968.3	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBA3 ( NM_003968.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003959.3 Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03627
Clone ID Related Accession (Same CDS sequence)	NM_003968.3 , NM_003968.4
Accession Version	NM_003968.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 catalytic subunit isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD700642.1, BC022853.1, AK002159.1 and AL117566.1. On Nov 23, 2018 this sequence version replaced NM_003968.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.773118.1, SRR3476690.904367.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361055.9/ ENSP00000354340.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGATG GCGAGGAGCC GGAGAAGAAA AGAAGGAGAA TAGAGGAGCT GCTGGCTGAG 
AAAATGGCTG TTGATGGTGG GTGTGGGGAC ACTGGAGACT GGGAAGGTCG CTGGAACCAT 
GTAAAGAAGT TCCTCGAGCG ATCTGGACCC TTCACACACC CTGATTTCGA ACCGAGCACT 
GAATCTCTCC AGTTCTTGTT AGATACATGT AAAGTTCTAG TCATTGGAGC TGGCGGCTTA 
GGATGTGAGC TCCTGAAAAA TCTGGCCTTG TCTGGTTTTA GACAGATTCA TGTTATAGAT 
ATGGACACTA TAGATGTTTC CAATCTAAAT AGGCAGTTTT TATTTAGGCC TAAAGATATT 
GGAAGACCTA AGGCTGAAGT TGCTGCAGAA TTTCTAAATG ACAGAGTTCC TAATTGCAAT 
GTAGTTCCAC ATTTCAACAA GATTCAAGAT TTTAACGACA CTTTCTATCG ACAATTTCAT 
ATTATTGTAT GTGGACTGGA CTCTATCATC GCCAGAAGAT GGATAAATGG CATGCTGATA 
TCTCTTCTAA ATTATGAAGA TGGTGTCTTA GATCCAAGCT CCATTGTCCC TTTGATAGAT 
GGGGGGACAG AAGGTTTTAA AGGAAATGCC CGGGTGATTC TGCCTGGAAT GACTGCTTGT 
ATCGAATGCA CGCTGGAACT TTATCCACCA CAGGTTAATT TTCCCATGTG CACCATTGCA 
TCTATGCCCA GGCTACCAGA ACACTGTATT GAGTATGTAA GGATGTTGCA GTGGCCTAAG 
GAGCAGCCTT TTGGAGAAGG GGTTCCATTA GATGGAGATG ATCCTGAACA TATACAATGG 
ATTTTCCAAA AATCCCTAGA GAGAGCATCA CAATATAATA TTAGGGGTGT TACGTATAGG 
CTCACTCAAG GGGTAGTAAA AAGAATCATT CCTGCAGTAG CTTCCACAAA TGCAGTCATT 
GCAGCTGTGT GTGCCACTGA GGTTTTTAAA ATAGCCACAA GTGCATACAT TCCCTTGAAT 
AATTACTTGG TGTTTAATGA TGTAGATGGG CTGTATACAT ACACATTTGA AGCAGAAAGA 
AAGGAAAACT GCCCAGCTTG TAGCCAGCTT CCTCAAAATA TTCAGTTTTC TCCATCAGCT 
AAACTACAGG AGGTTTTGGA TTATCTAACC AATAGTGCTT CTCTGCAAAT GAAATCTCCA 
GCCATCACAG CCACCCTAGA GGGAAAAAAT AGAACACTTT ACTTACAGTC GGTAACCTCT 
ATTGAAGAAC GAACAAGGCC AAATCTCTCC AAAACATTGA AAGAATTGGG GCTTGTTGAT 
GGACAAGAAC TGGCGGTTGC TGATGTCACC ACCCCACAGA CTGTACTATT CAAACTTCAT 
TTTACTTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003959.3
CDS	13..1404
Translation	MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Target ORF information:

RefSeq Version	NM_003968.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003968.4

ORF Insert Sequence:

ATGGCGGATG GCGAGGAGCC GGAGAAGAAA AGAAGGAGAA TAGAGGAGCT GCTGGCTGAG 
AAAATGGCTG TTGATGGTGG GTGTGGGGAC ACTGGAGACT GGGAAGGTCG CTGGAACCAT 
GTAAAGAAGT TCCTCGAGCG ATCTGGACCC TTCACACACC CTGATTTCGA ACCGAGCACT 
GAATCTCTCC AGTTCTTGTT AGATACATGT AAAGTTCTAG TCATTGGAGC TGGCGGCTTA 
GGATGTGAGC TCCTGAAAAA TCTGGCCTTG TCTGGTTTTA GACAGATTCA TGTTATAGAT 
ATGGACACTA TAGATGTTTC CAATCTAAAT AGGCAGTTTT TATTTAGGCC TAAAGATATT 
GGAAGACCTA AGGCTGAAGT TGCTGCAGAA TTTCTAAATG ACAGAGTTCC TAATTGCAAT 
GTAGTTCCAC ATTTCAACAA GATTCAAGAT TTTAACGACA CTTTCTATCG ACAATTTCAT 
ATTATTGTAT GTGGACTGGA CTCTATCATC GCCAGAAGAT GGATAAATGG CATGCTGATA 
TCTCTTCTAA ATTATGAAGA TGGTGTCTTA GATCCAAGCT CCATTGTCCC TTTGATAGAT 
GGGGGGACAG AAGGTTTTAA AGGAAATGCC CGGGTGATTC TGCCTGGAAT GACTGCTTGT 
ATCGAATGCA CGCTGGAACT TTATCCACCA CAGGTTAATT TTCCCATGTG CACCATTGCA 
TCTATGCCCA GGCTACCAGA ACACTGTATT GAGTATGTAA GGATGTTGCA GTGGCCTAAG 
GAGCAGCCTT TTGGAGAAGG GGTTCCATTA GATGGAGATG ATCCTGAACA TATACAATGG 
ATTTTCCAAA AATCCCTAGA GAGAGCATCA CAATATAATA TTAGGGGTGT TACGTATAGG 
CTCACTCAAG GGGTAGTAAA AAGAATCATT CCTGCAGTAG CTTCCACAAA TGCAGTCATT 
GCAGCTGTGT GTGCCACTGA GGTTTTTAAA ATAGCCACAA GTGCATACAT TCCCTTGAAT 
AATTACTTGG TGTTTAATGA TGTAGATGGG CTGTATACAT ACACATTTGA AGCAGAAAGA 
AAGGAAAACT GCCCAGCTTG TAGCCAGCTT CCTCAAAATA TTCAGTTTTC TCCATCAGCT 
AAACTACAGG AGGTTTTGGA TTATCTAACC AATAGTGCTT CTCTGCAAAT GAAATCTCCA 
GCCATCACAG CCACCCTAGA GGGAAAAAAT AGAACACTTT ACTTACAGTC GGTAACCTCT 
ATTGAAGAAC GAACAAGGCC AAATCTCTCC AAAACATTGA AAGAATTGGG GCTTGTTGAT 
GGACAAGAAC TGGCGGTTGC TGATGTCACC ACCCCACAGA CTGTACTATT CAAACTTCAT 
TTTACTTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA3 ( NM_198195.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_937838.1 Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02511
Clone ID Related Accession (Same CDS sequence)	NM_198195.2 , NM_198195.1
Accession Version	NM_198195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 catalytic subunit isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD700642.1, AK002159.1 and AL117566.1. On May 31, 2019 this sequence version replaced NM_198195.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, as compared to variant 1. The encoded isoform (2) is thus missing an internal segment in the N-terminal region, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.100398.1, SRR1660805.248576.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG GCGAGGAGCC AATGGCTGTT GATGGTGGGT GTGGGGACAC TGGAGACTGG 
GAAGGTCGCT GGAACCATGT AAAGAAGTTC CTCGAGCGAT CTGGACCCTT CACACACCCT 
GATTTCGAAC CGAGCACTGA ATCTCTCCAG TTCTTGTTAG ATACATGTAA AGTTCTAGTC 
ATTGGAGCTG GCGGCTTAGG ATGTGAGCTC CTGAAAAATC TGGCCTTGTC TGGTTTTAGA 
CAGATTCATG TTATAGATAT GGACACTATA GATGTTTCCA ATCTAAATAG GCAGTTTTTA 
TTTAGGCCTA AAGATATTGG AAGACCTAAG GCTGAAGTTG CTGCAGAATT TCTAAATGAC 
AGAGTTCCTA ATTGCAATGT AGTTCCACAT TTCAACAAGA TTCAAGATTT TAACGACACT 
TTCTATCGAC AATTTCATAT TATTGTATGT GGACTGGACT CTATCATCGC CAGAAGATGG 
ATAAATGGCA TGCTGATATC TCTTCTAAAT TATGAAGATG GTGTCTTAGA TCCAAGCTCC 
ATTGTCCCTT TGATAGATGG GGGGACAGAA GGTTTTAAAG GAAATGCCCG GGTGATTCTG 
CCTGGAATGA CTGCTTGTAT CGAATGCACG CTGGAACTTT ATCCACCACA GGTTAATTTT 
CCCATGTGCA CCATTGCATC TATGCCCAGG CTACCAGAAC ACTGTATTGA GTATGTAAGG 
ATGTTGCAGT GGCCTAAGGA GCAGCCTTTT GGAGAAGGGG TTCCATTAGA TGGAGATGAT 
CCTGAACATA TACAATGGAT TTTCCAAAAA TCCCTAGAGA GAGCATCACA ATATAATATT 
AGGGGTGTTA CGTATAGGCT CACTCAAGGG GTAGTAAAAA GAATCATTCC TGCAGTAGCT 
TCCACAAATG CAGTCATTGC AGCTGTGTGT GCCACTGAGG TTTTTAAAAT AGCCACAAGT 
GCATACATTC CCTTGAATAA TTACTTGGTG TTTAATGATG TAGATGGGCT GTATACATAC 
ACATTTGAAG CAGAAAGAAA GGAAAACTGC CCAGCTTGTA GCCAGCTTCC TCAAAATATT 
CAGTTTTCTC CATCAGCTAA ACTACAGGAG GTTTTGGATT ATCTAACCAA TAGTGCTTCT 
CTGCAAATGA AATCTCCAGC CATCACAGCC ACCCTAGAGG GAAAAAATAG AACACTTTAC 
TTACAGTCGG TAACCTCTAT TGAAGAACGA ACAAGGCCAA ATCTCTCCAA AACATTGAAA 
GAATTGGGGC TTGTTGATGG ACAAGAACTG GCGGTTGCTG ATGTCACCAC CCCACAGACT 
GTACTATTCA AACTTCATTT TACTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937838.1
CDS	13..1362
Translation	MADGEEPMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Target ORF information:

RefSeq Version	NM_198195.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198195.2

ORF Insert Sequence:

ATGGCGGATG GCGAGGAGCC AATGGCTGTT GATGGTGGGT GTGGGGACAC TGGAGACTGG 
GAAGGTCGCT GGAACCATGT AAAGAAGTTC CTCGAGCGAT CTGGACCCTT CACACACCCT 
GATTTCGAAC CGAGCACTGA ATCTCTCCAG TTCTTGTTAG ATACATGTAA AGTTCTAGTC 
ATTGGAGCTG GCGGCTTAGG ATGTGAGCTC CTGAAAAATC TGGCCTTGTC TGGTTTTAGA 
CAGATTCATG TTATAGATAT GGACACTATA GATGTTTCCA ATCTAAATAG GCAGTTTTTA 
TTTAGGCCTA AAGATATTGG AAGACCTAAG GCTGAAGTTG CTGCAGAATT TCTAAATGAC 
AGAGTTCCTA ATTGCAATGT AGTTCCACAT TTCAACAAGA TTCAAGATTT TAACGACACT 
TTCTATCGAC AATTTCATAT TATTGTATGT GGACTGGACT CTATCATCGC CAGAAGATGG 
ATAAATGGCA TGCTGATATC TCTTCTAAAT TATGAAGATG GTGTCTTAGA TCCAAGCTCC 
ATTGTCCCTT TGATAGATGG GGGGACAGAA GGTTTTAAAG GAAATGCCCG GGTGATTCTG 
CCTGGAATGA CTGCTTGTAT CGAATGCACG CTGGAACTTT ATCCACCACA GGTTAATTTT 
CCCATGTGCA CCATTGCATC TATGCCCAGG CTACCAGAAC ACTGTATTGA GTATGTAAGG 
ATGTTGCAGT GGCCTAAGGA GCAGCCTTTT GGAGAAGGGG TTCCATTAGA TGGAGATGAT 
CCTGAACATA TACAATGGAT TTTCCAAAAA TCCCTAGAGA GAGCATCACA ATATAATATT 
AGGGGTGTTA CGTATAGGCT CACTCAAGGG GTAGTAAAAA GAATCATTCC TGCAGTAGCT 
TCCACAAATG CAGTCATTGC AGCTGTGTGT GCCACTGAGG TTTTTAAAAT AGCCACAAGT 
GCATACATTC CCTTGAATAA TTACTTGGTG TTTAATGATG TAGATGGGCT GTATACATAC 
ACATTTGAAG CAGAAAGAAA GGAAAACTGC CCAGCTTGTA GCCAGCTTCC TCAAAATATT 
CAGTTTTCTC CATCAGCTAA ACTACAGGAG GTTTTGGATT ATCTAACCAA TAGTGCTTCT 
CTGCAAATGA AATCTCCAGC CATCACAGCC ACCCTAGAGG GAAAAAATAG AACACTTTAC 
TTACAGTCGG TAACCTCTAT TGAAGAACGA ACAAGGCCAA ATCTCTCCAA AACATTGAAA 
GAATTGGGGC TTGTTGATGG ACAAGAACTG GCGGTTGCTG ATGTCACCAC CCCACAGACT 
GTACTATTCA AACTTCATTT TACTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA3 ( NM_198195.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_937838.1 Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02511
Clone ID Related Accession (Same CDS sequence)	NM_198195.2 , NM_198195.1
Accession Version	NM_198195.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-02
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 catalytic subunit isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD700642.1, AK002159.1 and AL117566.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, as compared to variant 1. The encoded isoform (2) is thus missing an internal segment in the N-terminal region, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.174502.1, SRR1660803.42134.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG GCGAGGAGCC AATGGCTGTT GATGGTGGGT GTGGGGACAC TGGAGACTGG 
GAAGGTCGCT GGAACCATGT AAAGAAGTTC CTCGAGCGAT CTGGACCCTT CACACACCCT 
GATTTCGAAC CGAGCACTGA ATCTCTCCAG TTCTTGTTAG ATACATGTAA AGTTCTAGTC 
ATTGGAGCTG GCGGCTTAGG ATGTGAGCTC CTGAAAAATC TGGCCTTGTC TGGTTTTAGA 
CAGATTCATG TTATAGATAT GGACACTATA GATGTTTCCA ATCTAAATAG GCAGTTTTTA 
TTTAGGCCTA AAGATATTGG AAGACCTAAG GCTGAAGTTG CTGCAGAATT TCTAAATGAC 
AGAGTTCCTA ATTGCAATGT AGTTCCACAT TTCAACAAGA TTCAAGATTT TAACGACACT 
TTCTATCGAC AATTTCATAT TATTGTATGT GGACTGGACT CTATCATCGC CAGAAGATGG 
ATAAATGGCA TGCTGATATC TCTTCTAAAT TATGAAGATG GTGTCTTAGA TCCAAGCTCC 
ATTGTCCCTT TGATAGATGG GGGGACAGAA GGTTTTAAAG GAAATGCCCG GGTGATTCTG 
CCTGGAATGA CTGCTTGTAT CGAATGCACG CTGGAACTTT ATCCACCACA GGTTAATTTT 
CCCATGTGCA CCATTGCATC TATGCCCAGG CTACCAGAAC ACTGTATTGA GTATGTAAGG 
ATGTTGCAGT GGCCTAAGGA GCAGCCTTTT GGAGAAGGGG TTCCATTAGA TGGAGATGAT 
CCTGAACATA TACAATGGAT TTTCCAAAAA TCCCTAGAGA GAGCATCACA ATATAATATT 
AGGGGTGTTA CGTATAGGCT CACTCAAGGG GTAGTAAAAA GAATCATTCC TGCAGTAGCT 
TCCACAAATG CAGTCATTGC AGCTGTGTGT GCCACTGAGG TTTTTAAAAT AGCCACAAGT 
GCATACATTC CCTTGAATAA TTACTTGGTG TTTAATGATG TAGATGGGCT GTATACATAC 
ACATTTGAAG CAGAAAGAAA GGAAAACTGC CCAGCTTGTA GCCAGCTTCC TCAAAATATT 
CAGTTTTCTC CATCAGCTAA ACTACAGGAG GTTTTGGATT ATCTAACCAA TAGTGCTTCT 
CTGCAAATGA AATCTCCAGC CATCACAGCC ACCCTAGAGG GAAAAAATAG AACACTTTAC 
TTACAGTCGG TAACCTCTAT TGAAGAACGA ACAAGGCCAA ATCTCTCCAA AACATTGAAA 
GAATTGGGGC TTGTTGATGG ACAAGAACTG GCGGTTGCTG ATGTCACCAC CCCACAGACT 
GTACTATTCA AACTTCATTT TACTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937838.1
CDS	21..1370
Translation	MADGEEPMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Target ORF information:

RefSeq Version	NM_198195.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198195.1

ORF Insert Sequence:

ATGGCGGATG GCGAGGAGCC AATGGCTGTT GATGGTGGGT GTGGGGACAC TGGAGACTGG 
GAAGGTCGCT GGAACCATGT AAAGAAGTTC CTCGAGCGAT CTGGACCCTT CACACACCCT 
GATTTCGAAC CGAGCACTGA ATCTCTCCAG TTCTTGTTAG ATACATGTAA AGTTCTAGTC 
ATTGGAGCTG GCGGCTTAGG ATGTGAGCTC CTGAAAAATC TGGCCTTGTC TGGTTTTAGA 
CAGATTCATG TTATAGATAT GGACACTATA GATGTTTCCA ATCTAAATAG GCAGTTTTTA 
TTTAGGCCTA AAGATATTGG AAGACCTAAG GCTGAAGTTG CTGCAGAATT TCTAAATGAC 
AGAGTTCCTA ATTGCAATGT AGTTCCACAT TTCAACAAGA TTCAAGATTT TAACGACACT 
TTCTATCGAC AATTTCATAT TATTGTATGT GGACTGGACT CTATCATCGC CAGAAGATGG 
ATAAATGGCA TGCTGATATC TCTTCTAAAT TATGAAGATG GTGTCTTAGA TCCAAGCTCC 
ATTGTCCCTT TGATAGATGG GGGGACAGAA GGTTTTAAAG GAAATGCCCG GGTGATTCTG 
CCTGGAATGA CTGCTTGTAT CGAATGCACG CTGGAACTTT ATCCACCACA GGTTAATTTT 
CCCATGTGCA CCATTGCATC TATGCCCAGG CTACCAGAAC ACTGTATTGA GTATGTAAGG 
ATGTTGCAGT GGCCTAAGGA GCAGCCTTTT GGAGAAGGGG TTCCATTAGA TGGAGATGAT 
CCTGAACATA TACAATGGAT TTTCCAAAAA TCCCTAGAGA GAGCATCACA ATATAATATT 
AGGGGTGTTA CGTATAGGCT CACTCAAGGG GTAGTAAAAA GAATCATTCC TGCAGTAGCT 
TCCACAAATG CAGTCATTGC AGCTGTGTGT GCCACTGAGG TTTTTAAAAT AGCCACAAGT 
GCATACATTC CCTTGAATAA TTACTTGGTG TTTAATGATG TAGATGGGCT GTATACATAC 
ACATTTGAAG CAGAAAGAAA GGAAAACTGC CCAGCTTGTA GCCAGCTTCC TCAAAATATT 
CAGTTTTCTC CATCAGCTAA ACTACAGGAG GTTTTGGATT ATCTAACCAA TAGTGCTTCT 
CTGCAAATGA AATCTCCAGC CATCACAGCC ACCCTAGAGG GAAAAAATAG AACACTTTAC 
TTACAGTCGG TAACCTCTAT TGAAGAACGA ACAAGGCCAA ATCTCTCCAA AACATTGAAA 
GAATTGGGGC TTGTTGATGG ACAAGAACTG GCGGTTGCTG ATGTCACCAC CCCACAGACT 
GTACTATTCA AACTTCATTT TACTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA3 ( NM_003968.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003959.3 Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 1, mRNA.

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CloneID	OHu03627
Clone ID Related Accession (Same CDS sequence)	NM_003968.3 , NM_003968.4
Accession Version	NM_003968.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 catalytic subunit isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD700642.1, BC022853.1, AK002159.1 and AL117566.1. On Oct 30, 2003 this sequence version replaced NM_003968.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL117566.1, SRR1660807.69159.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG GCGAGGAGCC GGAGAAGAAA AGAAGGAGAA TAGAGGAGCT GCTGGCTGAG 
AAAATGGCTG TTGATGGTGG GTGTGGGGAC ACTGGAGACT GGGAAGGTCG CTGGAACCAT 
GTAAAGAAGT TCCTCGAGCG ATCTGGACCC TTCACACACC CTGATTTCGA ACCGAGCACT 
GAATCTCTCC AGTTCTTGTT AGATACATGT AAAGTTCTAG TCATTGGAGC TGGCGGCTTA 
GGATGTGAGC TCCTGAAAAA TCTGGCCTTG TCTGGTTTTA GACAGATTCA TGTTATAGAT 
ATGGACACTA TAGATGTTTC CAATCTAAAT AGGCAGTTTT TATTTAGGCC TAAAGATATT 
GGAAGACCTA AGGCTGAAGT TGCTGCAGAA TTTCTAAATG ACAGAGTTCC TAATTGCAAT 
GTAGTTCCAC ATTTCAACAA GATTCAAGAT TTTAACGACA CTTTCTATCG ACAATTTCAT 
ATTATTGTAT GTGGACTGGA CTCTATCATC GCCAGAAGAT GGATAAATGG CATGCTGATA 
TCTCTTCTAA ATTATGAAGA TGGTGTCTTA GATCCAAGCT CCATTGTCCC TTTGATAGAT 
GGGGGGACAG AAGGTTTTAA AGGAAATGCC CGGGTGATTC TGCCTGGAAT GACTGCTTGT 
ATCGAATGCA CGCTGGAACT TTATCCACCA CAGGTTAATT TTCCCATGTG CACCATTGCA 
TCTATGCCCA GGCTACCAGA ACACTGTATT GAGTATGTAA GGATGTTGCA GTGGCCTAAG 
GAGCAGCCTT TTGGAGAAGG GGTTCCATTA GATGGAGATG ATCCTGAACA TATACAATGG 
ATTTTCCAAA AATCCCTAGA GAGAGCATCA CAATATAATA TTAGGGGTGT TACGTATAGG 
CTCACTCAAG GGGTAGTAAA AAGAATCATT CCTGCAGTAG CTTCCACAAA TGCAGTCATT 
GCAGCTGTGT GTGCCACTGA GGTTTTTAAA ATAGCCACAA GTGCATACAT TCCCTTGAAT 
AATTACTTGG TGTTTAATGA TGTAGATGGG CTGTATACAT ACACATTTGA AGCAGAAAGA 
AAGGAAAACT GCCCAGCTTG TAGCCAGCTT CCTCAAAATA TTCAGTTTTC TCCATCAGCT 
AAACTACAGG AGGTTTTGGA TTATCTAACC AATAGTGCTT CTCTGCAAAT GAAATCTCCA 
GCCATCACAG CCACCCTAGA GGGAAAAAAT AGAACACTTT ACTTACAGTC GGTAACCTCT 
ATTGAAGAAC GAACAAGGCC AAATCTCTCC AAAACATTGA AAGAATTGGG GCTTGTTGAT 
GGACAAGAAC TGGCGGTTGC TGATGTCACC ACCCCACAGA CTGTACTATT CAAACTTCAT 
TTTACTTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003959.3
CDS	21..1412
Translation	MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Target ORF information:

RefSeq Version	NM_003968.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003968.3

ORF Insert Sequence:

ATGGCGGATG GCGAGGAGCC GGAGAAGAAA AGAAGGAGAA TAGAGGAGCT GCTGGCTGAG 
AAAATGGCTG TTGATGGTGG GTGTGGGGAC ACTGGAGACT GGGAAGGTCG CTGGAACCAT 
GTAAAGAAGT TCCTCGAGCG ATCTGGACCC TTCACACACC CTGATTTCGA ACCGAGCACT 
GAATCTCTCC AGTTCTTGTT AGATACATGT AAAGTTCTAG TCATTGGAGC TGGCGGCTTA 
GGATGTGAGC TCCTGAAAAA TCTGGCCTTG TCTGGTTTTA GACAGATTCA TGTTATAGAT 
ATGGACACTA TAGATGTTTC CAATCTAAAT AGGCAGTTTT TATTTAGGCC TAAAGATATT 
GGAAGACCTA AGGCTGAAGT TGCTGCAGAA TTTCTAAATG ACAGAGTTCC TAATTGCAAT 
GTAGTTCCAC ATTTCAACAA GATTCAAGAT TTTAACGACA CTTTCTATCG ACAATTTCAT 
ATTATTGTAT GTGGACTGGA CTCTATCATC GCCAGAAGAT GGATAAATGG CATGCTGATA 
TCTCTTCTAA ATTATGAAGA TGGTGTCTTA GATCCAAGCT CCATTGTCCC TTTGATAGAT 
GGGGGGACAG AAGGTTTTAA AGGAAATGCC CGGGTGATTC TGCCTGGAAT GACTGCTTGT 
ATCGAATGCA CGCTGGAACT TTATCCACCA CAGGTTAATT TTCCCATGTG CACCATTGCA 
TCTATGCCCA GGCTACCAGA ACACTGTATT GAGTATGTAA GGATGTTGCA GTGGCCTAAG 
GAGCAGCCTT TTGGAGAAGG GGTTCCATTA GATGGAGATG ATCCTGAACA TATACAATGG 
ATTTTCCAAA AATCCCTAGA GAGAGCATCA CAATATAATA TTAGGGGTGT TACGTATAGG 
CTCACTCAAG GGGTAGTAAA AAGAATCATT CCTGCAGTAG CTTCCACAAA TGCAGTCATT 
GCAGCTGTGT GTGCCACTGA GGTTTTTAAA ATAGCCACAA GTGCATACAT TCCCTTGAAT 
AATTACTTGG TGTTTAATGA TGTAGATGGG CTGTATACAT ACACATTTGA AGCAGAAAGA 
AAGGAAAACT GCCCAGCTTG TAGCCAGCTT CCTCAAAATA TTCAGTTTTC TCCATCAGCT 
AAACTACAGG AGGTTTTGGA TTATCTAACC AATAGTGCTT CTCTGCAAAT GAAATCTCCA 
GCCATCACAG CCACCCTAGA GGGAAAAAAT AGAACACTTT ACTTACAGTC GGTAACCTCT 
ATTGAAGAAC GAACAAGGCC AAATCTCTCC AAAACATTGA AAGAATTGGG GCTTGTTGAT 
GGACAAGAAC TGGCGGTTGC TGATGTCACC ACCCCACAGA CTGTACTATT CAAACTTCAT 
TTTACTTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA3 ( NM_001363861.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350790.1 Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 3, mRNA.

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CloneID	OHu62400
Clone ID Related Accession (Same CDS sequence)	XM_011534211.1 , NM_001363861.1
Accession Version	NM_001363861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 catalytic subunit isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092060.2 and AC109587.2. On Jun 3, 2018 this sequence version replaced XM_011534211.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.191.1, SRR1660809.42215.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATG GCGAGGAGCC AATGGCTGTT GATGGTGGGT GTGGGGACAC TGGAGACTGG 
GAAGGTCGCT GGAACCATGT AAAGAAGTTC CTCGAGCGAT CTGGACCCTT CACACACCCT 
GATTTCGAAC CGAGCACTGA AGCCTTGTCT GGTTTTAGAC AGATTCATGT TATAGATATG 
GACACTATAG ATGTTTCCAA TCTAAATAGG CAGTTTTTAT TTAGGCCTAA AGATATTGGA 
AGACCTAAGG CTGAAGTTGC TGCAGAATTT CTAAATGACA GAGTTCCTAA TTGCAATGTA 
GTTCCACATT TCAACAAGAT TCAAGATTTT AACGACACTT TCTATCGACA ATTTCATATT 
ATTGTATGTG GACTGGACTC TATCATCGCC AGAAGATGGA TAAATGGCAT GCTGATATCT 
CTTCTAAATT ATGAAGATGG TGTCTTAGAT CCAAGCTCCA TTGTCCCTTT GATAGATGGG 
GGGACAGAAG GTTTTAAAGG AAATGCCCGG GTGATTCTGC CTGGAATGAC TGCTTGTATC 
GAATGCACGC TGGAACTTTA TCCACCACAG GTTAATTTTC CCATGTGCAC CATTGCATCT 
ATGCCCAGGC TACCAGAACA CTGTATTGAG TATGTAAGGA TGTTGCAGTG GCCTAAGGAG 
CAGCCTTTTG GAGAAGGGGT TCCATTAGAT GGAGATGATC CTGAACATAT ACAATGGATT 
TTCCAAAAAT CCCTAGAGAG AGCATCACAA TATAATATTA GGGGTGTTAC GTATAGGCTC 
ACTCAAGGGG TAGTAAAAAG AATCATTCCT GCAGTAGCTT CCACAAATGC AGTCATTGCA 
GCTGTGTGTG CCACTGAGGT TTTTAAAATA GCCACAAGTG CATACATTCC CTTGAATAAT 
TACTTGGTGT TTAATGATGT AGATGGGCTG TATACATACA CATTTGAAGC AGAAAGAAAG 
GAAAACTGCC CAGCTTGTAG CCAGCTTCCT CAAAATATTC AGTTTTCTCC ATCAGCTAAA 
CTACAGGAGG TTTTGGATTA TCTAACCAAT AGTGCTTCTC TGCAAATGAA ATCTCCAGCC 
ATCACAGCCA CCCTAGAGGG AAAAAATAGA ACACTTTACT TACAGTCGGT AACCTCTATT 
GAAGAACGAA CAAGGCCAAA TCTCTCCAAA ACATTGAAAG AATTGGGGCT TGTTGATGGA 
CAAGAACTGG CGGTTGCTGA TGTCACCACC CCACAGACTG TACTATTCAA ACTTCATTTT 
ACTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350790.1
CDS	21..1289
Translation	MADGEEPMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTEALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Target ORF information:

RefSeq Version	NM_001363861.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363861.1

ORF Insert Sequence:

ATGGCGGATG GCGAGGAGCC AATGGCTGTT GATGGTGGGT GTGGGGACAC TGGAGACTGG 
GAAGGTCGCT GGAACCATGT AAAGAAGTTC CTCGAGCGAT CTGGACCCTT CACACACCCT 
GATTTCGAAC CGAGCACTGA AGCCTTGTCT GGTTTTAGAC AGATTCATGT TATAGATATG 
GACACTATAG ATGTTTCCAA TCTAAATAGG CAGTTTTTAT TTAGGCCTAA AGATATTGGA 
AGACCTAAGG CTGAAGTTGC TGCAGAATTT CTAAATGACA GAGTTCCTAA TTGCAATGTA 
GTTCCACATT TCAACAAGAT TCAAGATTTT AACGACACTT TCTATCGACA ATTTCATATT 
ATTGTATGTG GACTGGACTC TATCATCGCC AGAAGATGGA TAAATGGCAT GCTGATATCT 
CTTCTAAATT ATGAAGATGG TGTCTTAGAT CCAAGCTCCA TTGTCCCTTT GATAGATGGG 
GGGACAGAAG GTTTTAAAGG AAATGCCCGG GTGATTCTGC CTGGAATGAC TGCTTGTATC 
GAATGCACGC TGGAACTTTA TCCACCACAG GTTAATTTTC CCATGTGCAC CATTGCATCT 
ATGCCCAGGC TACCAGAACA CTGTATTGAG TATGTAAGGA TGTTGCAGTG GCCTAAGGAG 
CAGCCTTTTG GAGAAGGGGT TCCATTAGAT GGAGATGATC CTGAACATAT ACAATGGATT 
TTCCAAAAAT CCCTAGAGAG AGCATCACAA TATAATATTA GGGGTGTTAC GTATAGGCTC 
ACTCAAGGGG TAGTAAAAAG AATCATTCCT GCAGTAGCTT CCACAAATGC AGTCATTGCA 
GCTGTGTGTG CCACTGAGGT TTTTAAAATA GCCACAAGTG CATACATTCC CTTGAATAAT 
TACTTGGTGT TTAATGATGT AGATGGGCTG TATACATACA CATTTGAAGC AGAAAGAAAG 
GAAAACTGCC CAGCTTGTAG CCAGCTTCCT CAAAATATTC AGTTTTCTCC ATCAGCTAAA 
CTACAGGAGG TTTTGGATTA TCTAACCAAT AGTGCTTCTC TGCAAATGAA ATCTCCAGCC 
ATCACAGCCA CCCTAGAGGG AAAAAATAGA ACACTTTACT TACAGTCGGT AACCTCTATT 
GAAGAACGAA CAAGGCCAAA TCTCTCCAAA ACATTGAAAG AATTGGGGCT TGTTGATGGA 
CAAGAACTGG CGGTTGCTGA TGTCACCACC CCACAGACTG TACTATTCAA ACTTCATTTT 
ACTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA3 ( XM_011534210.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532512.1 Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant X1, mRNA.

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CloneID	OHu62399
Clone ID Related Accession (Same CDS sequence)	XM_011534210.1
Accession Version	XM_011534210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG GCGAGGAGCC GGAGAAGAAA AGAAGGAGAA TAGAGGAGCT GCTGGCTGAG 
AAAATGGCTG TTGATGGTGG GTGTGGGGAC ACTGGAGACT GGGAAGGTCG CTGGAACCAT 
GTAAAGAAGT TCCTCGAGCG ATCTGGACCC TTCACACACC CTGATTTCGA ACCGAGCACT 
GAAGCCTTGT CTGGTTTTAG ACAGATTCAT GTTATAGATA TGGACACTAT AGATGTTTCC 
AATCTAAATA GGCAGTTTTT ATTTAGGCCT AAAGATATTG GAAGACCTAA GGCTGAAGTT 
GCTGCAGAAT TTCTAAATGA CAGAGTTCCT AATTGCAATG TAGTTCCACA TTTCAACAAG 
ATTCAAGATT TTAACGACAC TTTCTATCGA CAATTTCATA TTATTGTATG TGGACTGGAC 
TCTATCATCG CCAGAAGATG GATAAATGGC ATGCTGATAT CTCTTCTAAA TTATGAAGAT 
GGTGTCTTAG ATCCAAGCTC CATTGTCCCT TTGATAGATG GGGGGACAGA AGGTTTTAAA 
GGAAATGCCC GGGTGATTCT GCCTGGAATG ACTGCTTGTA TCGAATGCAC GCTGGAACTT 
TATCCACCAC AGGTTAATTT TCCCATGTGC ACCATTGCAT CTATGCCCAG GCTACCAGAA 
CACTGTATTG AGTATGTAAG GATGTTGCAG TGGCCTAAGG AGCAGCCTTT TGGAGAAGGG 
GTTCCATTAG ATGGAGATGA TCCTGAACAT ATACAATGGA TTTTCCAAAA ATCCCTAGAG 
AGAGCATCAC AATATAATAT TAGGGGTGTT ACGTATAGGC TCACTCAAGG GGTAGTAAAA 
AGAATCATTC CTGCAGTAGC TTCCACAAAT GCAGTCATTG CAGCTGTGTG TGCCACTGAG 
GTTTTTAAAA TAGCCACAAG TGCATACATT CCCTTGAATA ATTACTTGGT GTTTAATGAT 
GTAGATGGGC TGTATACATA CACATTTGAA GCAGAAAGAA AGGAAAACTG CCCAGCTTGT 
AGCCAGCTTC CTCAAAATAT TCAGTTTTCT CCATCAGCTA AACTACAGGA GGTTTTGGAT 
TATCTAACCA ATAGTGCTTC TCTGCAAATG AAATCTCCAG CCATCACAGC CACCCTAGAG 
GGAAAAAATA GAACACTTTA CTTACAGTCG GTAACCTCTA TTGAAGAACG AACAAGGCCA 
AATCTCTCCA AAACATTGAA AGAATTGGGG CTTGTTGATG GACAAGAACT GGCGGTTGCT 
GATGTCACCA CCCCACAGAC TGTACTATTC AAACTTCATT TTACTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532512.1
CDS	16..1326
Translation	MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTEALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Target ORF information:

RefSeq Version	XM_011534210.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534210.1

ORF Insert Sequence:

ATGGCGGATG GCGAGGAGCC GGAGAAGAAA AGAAGGAGAA TAGAGGAGCT GCTGGCTGAG 
AAAATGGCTG TTGATGGTGG GTGTGGGGAC ACTGGAGACT GGGAAGGTCG CTGGAACCAT 
GTAAAGAAGT TCCTCGAGCG ATCTGGACCC TTCACACACC CTGATTTCGA ACCGAGCACT 
GAAGCCTTGT CTGGTTTTAG ACAGATTCAT GTTATAGATA TGGACACTAT AGATGTTTCC 
AATCTAAATA GGCAGTTTTT ATTTAGGCCT AAAGATATTG GAAGACCTAA GGCTGAAGTT 
GCTGCAGAAT TTCTAAATGA CAGAGTTCCT AATTGCAATG TAGTTCCACA TTTCAACAAG 
ATTCAAGATT TTAACGACAC TTTCTATCGA CAATTTCATA TTATTGTATG TGGACTGGAC 
TCTATCATCG CCAGAAGATG GATAAATGGC ATGCTGATAT CTCTTCTAAA TTATGAAGAT 
GGTGTCTTAG ATCCAAGCTC CATTGTCCCT TTGATAGATG GGGGGACAGA AGGTTTTAAA 
GGAAATGCCC GGGTGATTCT GCCTGGAATG ACTGCTTGTA TCGAATGCAC GCTGGAACTT 
TATCCACCAC AGGTTAATTT TCCCATGTGC ACCATTGCAT CTATGCCCAG GCTACCAGAA 
CACTGTATTG AGTATGTAAG GATGTTGCAG TGGCCTAAGG AGCAGCCTTT TGGAGAAGGG 
GTTCCATTAG ATGGAGATGA TCCTGAACAT ATACAATGGA TTTTCCAAAA ATCCCTAGAG 
AGAGCATCAC AATATAATAT TAGGGGTGTT ACGTATAGGC TCACTCAAGG GGTAGTAAAA 
AGAATCATTC CTGCAGTAGC TTCCACAAAT GCAGTCATTG CAGCTGTGTG TGCCACTGAG 
GTTTTTAAAA TAGCCACAAG TGCATACATT CCCTTGAATA ATTACTTGGT GTTTAATGAT 
GTAGATGGGC TGTATACATA CACATTTGAA GCAGAAAGAA AGGAAAACTG CCCAGCTTGT 
AGCCAGCTTC CTCAAAATAT TCAGTTTTCT CCATCAGCTA AACTACAGGA GGTTTTGGAT 
TATCTAACCA ATAGTGCTTC TCTGCAAATG AAATCTCCAG CCATCACAGC CACCCTAGAG 
GGAAAAAATA GAACACTTTA CTTACAGTCG GTAACCTCTA TTGAAGAACG AACAAGGCCA 
AATCTCTCCA AAACATTGAA AGAATTGGGG CTTGTTGATG GACAAGAACT GGCGGTTGCT 
GATGTCACCA CCCCACAGAC TGTACTATTC AAACTTCATT TTACTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA3 ( XM_011534211.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532513.1 Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant X2, mRNA.

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CloneID	OHu62400
Clone ID Related Accession (Same CDS sequence)	XM_011534211.1 , NM_001363861.1
Accession Version	XM_011534211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG GCGAGGAGCC AATGGCTGTT GATGGTGGGT GTGGGGACAC TGGAGACTGG 
GAAGGTCGCT GGAACCATGT AAAGAAGTTC CTCGAGCGAT CTGGACCCTT CACACACCCT 
GATTTCGAAC CGAGCACTGA AGCCTTGTCT GGTTTTAGAC AGATTCATGT TATAGATATG 
GACACTATAG ATGTTTCCAA TCTAAATAGG CAGTTTTTAT TTAGGCCTAA AGATATTGGA 
AGACCTAAGG CTGAAGTTGC TGCAGAATTT CTAAATGACA GAGTTCCTAA TTGCAATGTA 
GTTCCACATT TCAACAAGAT TCAAGATTTT AACGACACTT TCTATCGACA ATTTCATATT 
ATTGTATGTG GACTGGACTC TATCATCGCC AGAAGATGGA TAAATGGCAT GCTGATATCT 
CTTCTAAATT ATGAAGATGG TGTCTTAGAT CCAAGCTCCA TTGTCCCTTT GATAGATGGG 
GGGACAGAAG GTTTTAAAGG AAATGCCCGG GTGATTCTGC CTGGAATGAC TGCTTGTATC 
GAATGCACGC TGGAACTTTA TCCACCACAG GTTAATTTTC CCATGTGCAC CATTGCATCT 
ATGCCCAGGC TACCAGAACA CTGTATTGAG TATGTAAGGA TGTTGCAGTG GCCTAAGGAG 
CAGCCTTTTG GAGAAGGGGT TCCATTAGAT GGAGATGATC CTGAACATAT ACAATGGATT 
TTCCAAAAAT CCCTAGAGAG AGCATCACAA TATAATATTA GGGGTGTTAC GTATAGGCTC 
ACTCAAGGGG TAGTAAAAAG AATCATTCCT GCAGTAGCTT CCACAAATGC AGTCATTGCA 
GCTGTGTGTG CCACTGAGGT TTTTAAAATA GCCACAAGTG CATACATTCC CTTGAATAAT 
TACTTGGTGT TTAATGATGT AGATGGGCTG TATACATACA CATTTGAAGC AGAAAGAAAG 
GAAAACTGCC CAGCTTGTAG CCAGCTTCCT CAAAATATTC AGTTTTCTCC ATCAGCTAAA 
CTACAGGAGG TTTTGGATTA TCTAACCAAT AGTGCTTCTC TGCAAATGAA ATCTCCAGCC 
ATCACAGCCA CCCTAGAGGG AAAAAATAGA ACACTTTACT TACAGTCGGT AACCTCTATT 
GAAGAACGAA CAAGGCCAAA TCTCTCCAAA ACATTGAAAG AATTGGGGCT TGTTGATGGA 
CAAGAACTGG CGGTTGCTGA TGTCACCACC CCACAGACTG TACTATTCAA ACTTCATTTT 
ACTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532513.1
CDS	11..1279
Translation	MADGEEPMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTEALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Target ORF information:

RefSeq Version	XM_011534211.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534211.1

ORF Insert Sequence:

ATGGCGGATG GCGAGGAGCC AATGGCTGTT GATGGTGGGT GTGGGGACAC TGGAGACTGG 
GAAGGTCGCT GGAACCATGT AAAGAAGTTC CTCGAGCGAT CTGGACCCTT CACACACCCT 
GATTTCGAAC CGAGCACTGA AGCCTTGTCT GGTTTTAGAC AGATTCATGT TATAGATATG 
GACACTATAG ATGTTTCCAA TCTAAATAGG CAGTTTTTAT TTAGGCCTAA AGATATTGGA 
AGACCTAAGG CTGAAGTTGC TGCAGAATTT CTAAATGACA GAGTTCCTAA TTGCAATGTA 
GTTCCACATT TCAACAAGAT TCAAGATTTT AACGACACTT TCTATCGACA ATTTCATATT 
ATTGTATGTG GACTGGACTC TATCATCGCC AGAAGATGGA TAAATGGCAT GCTGATATCT 
CTTCTAAATT ATGAAGATGG TGTCTTAGAT CCAAGCTCCA TTGTCCCTTT GATAGATGGG 
GGGACAGAAG GTTTTAAAGG AAATGCCCGG GTGATTCTGC CTGGAATGAC TGCTTGTATC 
GAATGCACGC TGGAACTTTA TCCACCACAG GTTAATTTTC CCATGTGCAC CATTGCATCT 
ATGCCCAGGC TACCAGAACA CTGTATTGAG TATGTAAGGA TGTTGCAGTG GCCTAAGGAG 
CAGCCTTTTG GAGAAGGGGT TCCATTAGAT GGAGATGATC CTGAACATAT ACAATGGATT 
TTCCAAAAAT CCCTAGAGAG AGCATCACAA TATAATATTA GGGGTGTTAC GTATAGGCTC 
ACTCAAGGGG TAGTAAAAAG AATCATTCCT GCAGTAGCTT CCACAAATGC AGTCATTGCA 
GCTGTGTGTG CCACTGAGGT TTTTAAAATA GCCACAAGTG CATACATTCC CTTGAATAAT 
TACTTGGTGT TTAATGATGT AGATGGGCTG TATACATACA CATTTGAAGC AGAAAGAAAG 
GAAAACTGCC CAGCTTGTAG CCAGCTTCCT CAAAATATTC AGTTTTCTCC ATCAGCTAAA 
CTACAGGAGG TTTTGGATTA TCTAACCAAT AGTGCTTCTC TGCAAATGAA ATCTCCAGCC 
ATCACAGCCA CCCTAGAGGG AAAAAATAGA ACACTTTACT TACAGTCGGT AACCTCTATT 
GAAGAACGAA CAAGGCCAAA TCTCTCCAAA ACATTGAAAG AATTGGGGCT TGTTGATGGA 
CAAGAACTGG CGGTTGCTGA TGTCACCACC CCACAGACTG TACTATTCAA ACTTCATTTT 
ACTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neddylation pathway is up-regulated in human intrahepatic cholangiocarcinoma and serves as a potential therapeutic target.
Oncotarget5(17)7820-32(2014 Sep)
Gao Q,Yu GY,Shi JY,Li LH,Zhang WJ,Wang ZC,Yang LX,Duan M,Zhao H,Wang XY,Zhou J,Qiu SJ,Jeong LS,Jia LJ,Fan J

Suppression of tumor angiogenesis by targeting the protein neddylation pathway.
Cell death & disease5e1059(2014 Feb)
Yao WT,Wu JF,Yu GY,Wang R,Wang K,Li LH,Chen P,Jiang YN,Cheng H,Lee HW,Yu J,Qi H,Yu XJ,Wang P,Chu YW,Yang M,Hua ZC,Ying HQ,Hoffman RM,Jeong LS,Jia LJ

Mutations in UBA3 confer resistance to the NEDD8-activating enzyme inhibitor MLN4924 in human leukemic cells.
PloS one9(4)e93530(2014)
Xu GW,Toth JI,da Silva SR,Paiva SL,Lukkarila JL,Hurren R,Maclean N,Sukhai MA,Bhattacharjee RN,Goard CA,Medeiros B,Gunning PT,Dhe-Paganon S,Petroski MD,Schimmer AD

E2-binding surface on Uba3 ?-grasp domain undergoes a conformational transition.
Proteins80(10)2482-7(2012 Oct)
Elgin ES,S?kmen N,Peterson FC,Volkman BF,Da? C,Haas AL

Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network.
Genome research19(10)1905-11(2009 Oct)
Markson G,Kiel C,Hyde R,Brown S,Charalabous P,Bremm A,Semple J,Woodsmith J,Duley S,Salehi-Ashtiani K,Vidal M,Komander D,Serrano L,Lehner P,Sanderson CM

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UBA3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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