CH25H cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CH25H
Entrez Gene ID	9023
Full Name	cholesterol 25-hydroxylase
Synonyms	C25H
General protein information	Preferred Names cholesterol 25-hydroxylase Names cholesterol 25-hydroxylase cholesterol 25-monooxygenase h25OH
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q23.31
Summary	This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008].
Disorder MIM:	604551

mRNA and Protein(s)

mRNA	Protein	Name
NM_003956.3	NP_003947.1	cholesterol 25-hydroxylase
NM_003956.4	NP_003947.1	cholesterol 25-hydroxylase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CH25H	XP_003639977.1
Rattus norvegicus (Norway rat)	Ch25h	NP_001020586.1
Danio rerio (zebrafish)	ch25h	NP_001008652.1
Xenopus tropicalis (tropical clawed frog)	LOC100494906	XP_002935478.1
	CH25H	XP_001083208.1
Mus musculus (house mouse)	Ch25h	NP_034020.1
Homo sapiens (human)	CH25H	NP_003947.1
Pan troglodytes (chimpanzee)	CH25H	XP_507901.2
Bos taurus (cattle)	CH25H	NP_001068711.1
Gallus gallus (chicken)	CH25H	NP_001264283.1

Gene Ontology
Bibliography

Related articles in PubMed

Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function.
Zerbinati C, Caponecchia L, Puca R, Ciacciarelli M, Salacone P, Sebastianelli A, Pastore A, Palleschi G, Petrozza V, Porta N, Rago R, Carbone A, Iuliano L
Redox biology11111-117(2017 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We detected Cholesterol 25-hydroxylase, the enzyme responsible for 25-HC production, in human spermatozoa at the level of the neck and the post acrosomal area. Upon incubation with spermatozoa, 25-HC induced calcium and cholesterol transients in connection with the acrosomal reaction.
Title: Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function.

Exome analysis in 212 multiple sclerosis and 14 neuromyelitis optica patients identified a rare CH25H p.Q17H mutation in two NMO patients of Asian ancestry. In addition, association analysis of common CH25H variants.
Title: Analysis of CH25H in multiple sclerosis and neuromyelitis optica.

Infection with HCV causes up-regulation of interferon-inducible CH25H in vivo.
Title: Interferon-inducible cholesterol-25-hydroxylase restricts hepatitis C virus replication through blockage of membranous web formation.

These results demonstrate that CH25H constitutes a primary innate response against hepatitis C virus infection through regulating host lipid metabolism.
Title: Identification of Cholesterol 25-Hydroxylase as a Novel Host Restriction Factor and a Part of the Primary Innate Immune Responses against Hepatitis C Virus Infection.

Cholesterol 25-hydroxylase production by dendritic cells and macrophages is regulated by type I interferons.
Title: Cholesterol 25-hydroxylase production by dendritic cells and macrophages is regulated by type I interferons.

The following CH25H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CH25H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29872	NM_003956.4 Latest version!	Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu29872	NM_003956.3	Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CH25H ( NM_003956.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003947.1 Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA.

Price & Availability↑

CloneID	OHu29872
Clone ID Related Accession (Same CDS sequence)	NM_003956.3 , NM_003956.4
Accession Version	NM_003956.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	cholesterol 25-hydroxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017843.1. On May 13, 2006 this sequence version replaced NM_003956.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC017843.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGCC ACAACTGCTC CGACCCCCAG GTCCTTTGCA GCTCCGGGCA GCTGTTCCTG 
CAGCCCCTCT GGGACCACCT GAGGAGCTGG GAGGCCCTCC TACAGTCGCC CTTCTTCCCG 
GTCATCTTCT CCATCACCAC ATACGTGGGC TTTTGCCTGC CCTTCGTGGT CCTGGATATC 
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCTGACTT CTCGCCATCC 
GCGCAGCAGC TGCTACCTTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC 
CCCGTGACGC TGCTGCATTG GGCCCGCAGC CCGGCCCTCC TGCCCCACGA AGCTCCCGAG 
CTGCTCCTGC TGCTGCACCA CATCCTGTTC TGCCTGCTAC TCTTCGACAT GGAGTTCTTC 
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC 
CACCAGAACT CGTCCTCGTT CGCGCTGGCA ACGCAGTATA TGAGCGTCTG GGAACTGTTT 
TCTTTGGGCT TCTTCGACAT GATGAACGTC ACACTGCTCG GGTGCCACCC GCTCACCACC 
CTGACCTTCC ACGTGGTCAA CATCTGGCTT TCCGTGGAGG ACCACTCCGG CTACAACTTC 
CCTTGGTCCA CTCACAGACT GGTGCCCTTC GGGTGGTACG GGGGTGTGGT GCACCACGAC 
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA 
CTGGGAACGC TGCGGACTGC ATCTGTCCCA GCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003947.1
CDS	23..841
Translation	MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPAR

Target ORF information:

RefSeq Version	NM_003956.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003956.3

ORF Insert Sequence:

ATGAGCTGCC ACAACTGCTC CGACCCCCAG GTCCTTTGCA GCTCCGGGCA GCTGTTCCTG 
CAGCCCCTCT GGGACCACCT GAGGAGCTGG GAGGCCCTCC TACAGTCGCC CTTCTTCCCG 
GTCATCTTCT CCATCACCAC ATACGTGGGC TTTTGCCTGC CCTTCGTGGT CCTGGATATC 
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCTGACTT CTCGCCATCC 
GCGCAGCAGC TGCTACCTTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC 
CCCGTGACGC TGCTGCATTG GGCCCGCAGC CCGGCCCTCC TGCCCCACGA AGCTCCCGAG 
CTGCTCCTGC TGCTGCACCA CATCCTGTTC TGCCTGCTAC TCTTCGACAT GGAGTTCTTC 
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC 
CACCAGAACT CGTCCTCGTT CGCGCTGGCA ACGCAGTATA TGAGCGTCTG GGAACTGTTT 
TCTTTGGGCT TCTTCGACAT GATGAACGTC ACACTGCTCG GGTGCCACCC GCTCACCACC 
CTGACCTTCC ACGTGGTCAA CATCTGGCTT TCCGTGGAGG ACCACTCCGG CTACAACTTC 
CCTTGGTCCA CTCACAGACT GGTGCCCTTC GGGTGGTACG GGGGTGTGGT GCACCACGAC 
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA 
CTGGGAACGC TGCGGACTGC ATCTGTCCCA GCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CH25H ( NM_003956.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003947.1 Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA.

Price & Availability↑

CloneID	OHu29872
Clone ID Related Accession (Same CDS sequence)	NM_003956.3 , NM_003956.4
Accession Version	NM_003956.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	cholesterol 25-hydroxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513533.15 and BC017843.1. On May 17, 2019 this sequence version replaced NM_003956.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR1660809.218929.1, DRR138524.275451.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371852.4/ ENSP00000360918.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGCC ACAACTGCTC CGACCCCCAG GTCCTTTGCA GCTCCGGGCA GCTGTTCCTG 
CAGCCCCTCT GGGACCACCT GAGGAGCTGG GAGGCCCTCC TACAGTCGCC CTTCTTCCCG 
GTCATCTTCT CCATCACCAC ATACGTGGGC TTTTGCCTGC CCTTCGTGGT CCTGGATATC 
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCTGACTT CTCGCCATCC 
GCGCAGCAGC TGCTACCTTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC 
CCCGTGACGC TGCTGCATTG GGCCCGCAGC CCGGCCCTCC TGCCCCACGA AGCTCCCGAG 
CTGCTCCTGC TGCTGCACCA CATCCTGTTC TGCCTGCTAC TCTTCGACAT GGAGTTCTTC 
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC 
CACCAGAACT CGTCCTCGTT CGCGCTGGCA ACGCAGTATA TGAGCGTCTG GGAACTGTTT 
TCTTTGGGCT TCTTCGACAT GATGAACGTC ACACTGCTCG GGTGCCACCC GCTCACCACC 
CTGACCTTCC ACGTGGTCAA CATCTGGCTT TCCGTGGAGG ACCACTCCGG CTACAACTTC 
CCTTGGTCCA CTCACAGACT GGTGCCCTTC GGGTGGTACG GGGGTGTGGT GCACCACGAC 
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA 
CTGGGAACGC TGCGGACTGC ATCTGTCCCA GCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003947.1
CDS	26..844
Translation	MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPAR

Target ORF information:

RefSeq Version	NM_003956.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003956.4

ORF Insert Sequence:

ATGAGCTGCC ACAACTGCTC CGACCCCCAG GTCCTTTGCA GCTCCGGGCA GCTGTTCCTG 
CAGCCCCTCT GGGACCACCT GAGGAGCTGG GAGGCCCTCC TACAGTCGCC CTTCTTCCCG 
GTCATCTTCT CCATCACCAC ATACGTGGGC TTTTGCCTGC CCTTCGTGGT CCTGGATATC 
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCTGACTT CTCGCCATCC 
GCGCAGCAGC TGCTACCTTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC 
CCCGTGACGC TGCTGCATTG GGCCCGCAGC CCGGCCCTCC TGCCCCACGA AGCTCCCGAG 
CTGCTCCTGC TGCTGCACCA CATCCTGTTC TGCCTGCTAC TCTTCGACAT GGAGTTCTTC 
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC 
CACCAGAACT CGTCCTCGTT CGCGCTGGCA ACGCAGTATA TGAGCGTCTG GGAACTGTTT 
TCTTTGGGCT TCTTCGACAT GATGAACGTC ACACTGCTCG GGTGCCACCC GCTCACCACC 
CTGACCTTCC ACGTGGTCAA CATCTGGCTT TCCGTGGAGG ACCACTCCGG CTACAACTTC 
CCTTGGTCCA CTCACAGACT GGTGCCCTTC GGGTGGTACG GGGGTGTGGT GCACCACGAC 
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA 
CTGGGAACGC TGCGGACTGC ATCTGTCCCA GCGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function.
Redox biology11111-117(2017 04)
Zerbinati C,Caponecchia L,Puca R,Ciacciarelli M,Salacone P,Sebastianelli A,Pastore A,Palleschi G,Petrozza V,Porta N,Rago R,Carbone A,Iuliano L

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CH25H cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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