ATP6V0E1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP6V0E1
Entrez Gene ID	8992
Full Name	ATPase H+ transporting V0 subunit e1
Synonyms	ATP6H,ATP6V0E,M9.2,Vma21,Vma21p
General protein information	Preferred Names ATPase H+ transporting V0 subunit e1 Names V-type proton ATPase subunit e 1 ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 H(+)-transporting two-sector ATPase, subunit H V-ATPase 9.2 kDa membrane accessory protein V-ATPase H subunit V-ATPase M9.2 subunit V-ATPase subunit e 1 vacuolar ATP synthase subunit H vacuolar proton pump H subunit vacuolar proton pump subunit e 1 vacuolar proton-ATPase subunit M9.2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q35.1
Summary	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3. [provided by RefSeq, Jul 2008].
Disorder MIM:	603931

mRNA and Protein(s)

mRNA	Protein	Name
NM_003945.3	NP_003936.1	V-type proton ATPase subunit e 1
NM_003945.4	NP_003936.1	V-type proton ATPase subunit e 1

Pathways from BioSystems

Homologies

	ATP6V0E1	XP_001096225.1
Gallus gallus (chicken)	ATP6V0E1	NP_001091001.1
Homo sapiens (human)	ATP6V0E1	NP_003936.1
Pan troglodytes (chimpanzee)	ATP6V0E1	XP_003311011.1
Danio rerio (zebrafish)	atpv0e1	NP_001006038.1
Xenopus tropicalis (tropical clawed frog)	atp6v0e1	NP_001165157.1
Canis lupus familiaris (dog)	ATP6V0E1	NP_001003128.1
Rattus norvegicus (Norway rat)	Atp6v0e1	NP_446030.3
Bos taurus (cattle)	ATP6V0E1	NP_001029222.1
Mus musculus (house mouse)	Atp6v0e	NP_079548.1

Gene Ontology
Bibliography

Related articles in PubMed

Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts.
H?hn A, Sittig A, Jung T, Grimm S, Grune T
Free radical biology & medicine53(9)1760-9(2012 Nov)

Physical interaction between aldolase and vacuolar H+-ATPase is essential for the assembly and activity of the proton pump.
Lu M, Ammar D, Ives H, Albrecht F, Gluck SL
The Journal of biological chemistry282(34)24495-503(2007 Aug)

Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation.
Adachi K, Oiwa K, Nishizaka T, Furuike S, Noji H, Itoh H, Yoshida M, Kinosita K Jr
Cell130(2)309-21(2007 Jul)

Molecular cloning and characterization of a novel form of the human vacuolar H+-ATPase e-subunit: an essential proton pump component.
Blake-Palmer KG, Su Y, Smith AN, Karet FE
Gene393(1-2)94-100(2007 May)

Full-length coding sequences and mapping of porcine ATP6VOE and ATP5G1 genes.
Wang HL, Zhu ZM, Yerle M, Wu X, Wang H, Yang SL, Li K
Cytogenetic and genome research109(4)533(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

there is an important role for physical association between aldolase and the A, B and E subunits of V-ATPase in the regulation of the proton pump
Title: Physical interaction between aldolase and vacuolar H+-ATPase is essential for the assembly and activity of the proton pump.

Data demonstrate the physiological significance of the interaction between the E and H subunits of V-ATPase and extend previous studies on the arrangement of subunits on the peripheral stalk of V-ATPase.
Title: The amino-terminal domain of the E subunit of vacuolar H(+)-ATPase (V-ATPase) interacts with the H subunit and is required for V-ATPase function.

The following ATP6V0E1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP6V0E1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30806	NM_003945.4 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit e1 (ATP6V0E1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu30806	NM_003945.3	Homo sapiens ATPase H+ transporting V0 subunit e1 (ATP6V0E1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP6V0E1 ( NM_003945.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003936.1 Homo sapiens ATPase H+ transporting V0 subunit e1 (ATP6V0E1), mRNA.

Price & Availability↑

CloneID	OHu30806
Clone ID Related Accession (Same CDS sequence)	NM_003945.3 , NM_003945.4
Accession Version	NM_003945.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	V-type proton ATPase subunit e 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF691381.1, Y15286.1 and AI198465.1. On Jul 26, 2004 this sequence version replaced NM_003945.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.31594.1, SRR1163657.456953.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTATC ACGGCCTCAC TGTGCCTCTC ATTGTGATGA GCGTGTTCTG GGGCTTCGTC 
GGCTTCTTGG TGCCTTGGTT CATCCCTAAG GGTCCTAACC GGGGAGTTAT CATTACCATG 
TTGGTGACCT GTTCAGTTTG CTGCTATCTC TTTTGGCTGA TTGCAATTCT GGCCCAACTC 
AACCCTCTCT TTGGACCGCA ATTGAAAAAT GAAACCATCT GGTATCTGAA GTATCATTGG 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003936.1
CDS	102..347
Translation	MAYHGLTVPLIVMSVFWGFVGFLVPWFIPKGPNRGVIITMLVTCSVCCYLFWLIAILAQLNPLFGPQLKNETIWYLKYHWP

Target ORF information:

RefSeq Version	NM_003945.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit e1 (ATP6V0E1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003945.3

ORF Insert Sequence:

ATGGCGTATC ACGGCCTCAC TGTGCCTCTC ATTGTGATGA GCGTGTTCTG GGGCTTCGTC 
GGCTTCTTGG TGCCTTGGTT CATCCCTAAG GGTCCTAACC GGGGAGTTAT CATTACCATG 
TTGGTGACCT GTTCAGTTTG CTGCTATCTC TTTTGGCTGA TTGCAATTCT GGCCCAACTC 
AACCCTCTCT TTGGACCGCA ATTGAAAAAT GAAACCATCT GGTATCTGAA GTATCATTGG 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0E1 ( NM_003945.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003936.1 Homo sapiens ATPase H+ transporting V0 subunit e1 (ATP6V0E1), mRNA.

Price & Availability↑

CloneID	OHu30806
Clone ID Related Accession (Same CDS sequence)	NM_003945.3 , NM_003945.4
Accession Version	NM_003945.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	V-type proton ATPase subunit e 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF691381.1, Y15286.1, AI198465.1 and AC008429.6. On Nov 22, 2018 this sequence version replaced NM_003945.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG623204.1, SRR1803611.69943.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000519374.6/ ENSP00000429690.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTATC ACGGCCTCAC TGTGCCTCTC ATTGTGATGA GCGTGTTCTG GGGCTTCGTC 
GGCTTCTTGG TGCCTTGGTT CATCCCTAAG GGTCCTAACC GGGGAGTTAT CATTACCATG 
TTGGTGACCT GTTCAGTTTG CTGCTATCTC TTTTGGCTGA TTGCAATTCT GGCCCAACTC 
AACCCTCTCT TTGGACCGCA ATTGAAAAAT GAAACCATCT GGTATCTGAA GTATCATTGG 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003936.1
CDS	91..336
Translation	MAYHGLTVPLIVMSVFWGFVGFLVPWFIPKGPNRGVIITMLVTCSVCCYLFWLIAILAQLNPLFGPQLKNETIWYLKYHWP

Target ORF information:

RefSeq Version	NM_003945.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit e1 (ATP6V0E1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003945.4

ORF Insert Sequence:

ATGGCGTATC ACGGCCTCAC TGTGCCTCTC ATTGTGATGA GCGTGTTCTG GGGCTTCGTC 
GGCTTCTTGG TGCCTTGGTT CATCCCTAAG GGTCCTAACC GGGGAGTTAT CATTACCATG 
TTGGTGACCT GTTCAGTTTG CTGCTATCTC TTTTGGCTGA TTGCAATTCT GGCCCAACTC 
AACCCTCTCT TTGGACCGCA ATTGAAAAAT GAAACCATCT GGTATCTGAA GTATCATTGG 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts.
Free radical biology & medicine53(9)1760-9(2012 Nov)
H?hn A,Sittig A,Jung T,Grimm S,Grune T

Physical interaction between aldolase and vacuolar H+-ATPase is essential for the assembly and activity of the proton pump.
The Journal of biological chemistry282(34)24495-503(2007 Aug)
Lu M,Ammar D,Ives H,Albrecht F,Gluck SL

Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation.
Cell130(2)309-21(2007 Jul)
Adachi K,Oiwa K,Nishizaka T,Furuike S,Noji H,Itoh H,Yoshida M,Kinosita K Jr

Molecular cloning and characterization of a novel form of the human vacuolar H+-ATPase e-subunit: an essential proton pump component.
Gene393(1-2)94-100(2007 May)
Blake-Palmer KG,Su Y,Smith AN,Karet FE

Full-length coding sequences and mapping of porcine ATP6VOE and ATP5G1 genes.
Cytogenetic and genome research109(4)533(2005)
Wang HL,Zhu ZM,Yerle M,Wu X,Wang H,Yang SL,Li K

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ATP6V0E1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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