SEC16B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC16B
Entrez Gene ID	89866
Full Name	SEC16 homolog B, endoplasmic reticulum export factor
Synonyms	LZTR2,PGPR-p117,RGPR,RGPR-p117,SEC16S
General protein information	Preferred Names SEC16 homolog B, endoplasmic reticulum export factor Names protein transport protein Sec16B leucine zipper transcription regulator 2 protein SEC16 homolog B regucalcin gene promoter region-related protein p117 regucalcin gene promotor region related protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q25.2
Summary	SEC16B is a mammalian homolog of S. cerevisiae Sec16 that is required for organization of transitional endoplasmic reticulum (ER) sites and protein export (Bhattacharyya and Glick, 2007 [PubMed 17192411]).[supplied by OMIM, Jun 2009].
Disorder MIM:	612855

mRNA and Protein(s)

mRNA	Protein	Name
NM_001356500.1	NP_001343429.1	protein transport protein Sec16B isoform 3
NM_001356499.1	NP_001343428.1	protein transport protein Sec16B isoform 2
NM_033127.2	NP_149118.2	protein transport protein Sec16B
NM_033127.3	NP_149118.2	protein transport protein Sec16B isoform 1
NM_001356499.2	NP_001343428.1	protein transport protein Sec16B isoform 2

Pathways from BioSystems

Homologies

	SEC16B	XP_002802118.1
Rattus norvegicus (Norway rat)	Sec16b	NP_446023.1
Canis lupus familiaris (dog)	SEC16B	XP_005622544.1
Bos taurus (cattle)	SEC16B	NP_996854.2
Homo sapiens (human)	SEC16B	NP_149118.2
Mus musculus (house mouse)	Sec16b	NP_001153458.1
Gallus gallus (chicken)	SEC16B	NP_001026440.1
Xenopus tropicalis (tropical clawed frog)	sec16b	XP_004914083.1
Pan troglodytes (chimpanzee)	SEC16B	XP_514022.3

Gene Ontology
Bibliography

Related articles in PubMed

Validation of obesity susceptibility loci identified by genome-wide association studies in early childhood in South Brazilian children.
Zandon? MR, Sangalli CN, Campagnolo PD, Vitolo MR, Almeida S, Mattevi VS
Pediatric obesity12(1)85-92(2017 Feb)

Genetic variations in SEC16B, MC4R, MAP2K5 and KCTD15 were associated with childhood obesity and interacted with dietary behaviors in Chinese school-age population.
Lv D, Zhang DD, Wang H, Zhang Y, Liang L, Fu JF, Xiong F, Liu GL, Gong CX, Luo FH, Chen SK, Li ZL, Zhu YM
Gene560(2)149-55(2015 Apr)

Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche.
Perry JR, Day F, Elks CE, Sulem P, Thompson DJ, Ferreira T, He C, Chasman DI, Esko T, Thorleifsson G, Albrecht E, Ang WQ, Corre T, Cousminer DL, Feenstra B, Franceschini N, Ganna A, Johnson AD, Kjellqvist S, Lunetta KL, McMahon G, Nolte IM, Paternoster L, Porcu E, Smith AV, Stolk L, Teumer A, T?ernikova N, Tikkanen E, Ulivi S, Wagner EK, Amin N, Bierut LJ, Byrne EM, Hottenga JJ, Koller DL, Mangino M, Pers TH, Yerges-Armstrong LM, Zhao JH, Andrulis IL, Anton-Culver H, Atsma F, Bandinelli S, Beckmann MW, Benitez J, Blomqvist C, Bojesen SE, Bolla MK, Bonanni B, Brauch H, Brenner H, Buring JE, Chang-Claude J, Chanock S, Chen J, Chenevix-Trench G, Coll?e JM, Couch FJ, Couper D, Coveillo AD, Cox A, Czene K, D'adamo AP, Smith GD, De Vivo I, Demerath EW, Dennis J, Devilee P, Dieffenbach AK, Dunning AM, Eiriksdottir G, Eriksson JG, Fasching PA, Ferrucci L, Flesch-Janys D, Flyger H, Foroud T, Franke L, Garcia ME, Garc?a-Closas M, Geller F, de Geus EE, Giles GG, Gudbjartsson DF, Gudnason V, Gu?nel P, Guo S, Hall P, Hamann U, Haring R, Hartman CA, Heath AC, Hofman A, Hooning MJ, Hopper JL, Hu FB, Hunter DJ, Karasik D, Kiel DP, Knight JA, Kosma VM, Kutalik Z, Lai S, Lambrechts D, Lindblom A, M?gi R, Magnusson PK, Mannermaa A, Martin NG, Masson G, McArdle PF, McArdle WL, Melbye M, Michailidou K, Mihailov E, Milani L, Milne RL, Nevanlinna H, Neven P, Nohr EA, Oldehinkel AJ, Oostra BA, Palotie A, Peacock M, Pedersen NL, Peterlongo P, Peto J, Pharoah PD, Postma DS, Pouta A, Pylk?s K, Radice P, Ring S, Rivadeneira F, Robino A, Rose LM, Rudolph A, Salomaa V, Sanna S, Schlessinger D, Schmidt MK, Southey MC, Sovio U, Stampfer MJ, St?ckl D, Storniolo AM, Timpson NJ, Tyrer J, Visser JA, Vollenweider P, V?lzke H, Waeber G, Waldenberger M, Wallaschofski H, Wang Q, Willemsen G, Winqvist R, Wolffenbuttel BH, Wright MJ, , , , , , , Boomsma DI, Econs MJ, Khaw KT, Loos RJ, McCarthy MI, Montgomery GW, Rice JP, Streeten EA, Thorsteinsdottir U, van Duijn CM, Alizadeh BZ, Bergmann S, Boerwinkle E, Boyd HA, Crisponi L, Gasparini P, Gieger C, Harris TB, Ingelsson E, J?rvelin MR, Kraft P, Lawlor D, Metspalu A, Pennell CE, Ridker PM, Snieder H, S?rensen TI, Spector TD, Strachan DP, Uitterlinden AG, Wareham NJ, Widen E, Zygmunt M, Murray A, Easton DF, Stefansson K, Murabito JM, Ong KK
Nature514(7520)92-97(2014 Oct)

Meta-analysis of genome-wide association studies in East Asian-ancestry populations identifies four new loci for body mass index.
Wen W, Zheng W, Okada Y, Takeuchi F, Tabara Y, Hwang JY, Dorajoo R, Li H, Tsai FJ, Yang X, He J, Wu Y, He M, Zhang Y, Liang J, Guo X, Sheu WH, Delahanty R, Guo X, Kubo M, Yamamoto K, Ohkubo T, Go MJ, Liu JJ, Gan W, Chen CC, Gao Y, Li S, Lee NR, Wu C, Zhou X, Song H, Yao J, Lee IT, Long J, Tsunoda T, Akiyama K, Takashima N, Cho YS, Ong RT, Lu L, Chen CH, Tan A, Rice TK, Adair LS, Gui L, Allison M, Lee WJ, Cai Q, Isomura M, Umemura S, Kim YJ, Seielstad M, Hixson J, Xiang YB, Isono M, Kim BJ, Sim X, Lu W, Nabika T, Lee J, Lim WY, Gao YT, Takayanagi R, Kang DH, Wong TY, Hsiung CA, Wu IC, Juang JM, Shi J, Choi BY, Aung T, Hu F, Kim MK, Lim WY, Wang TD, Shin MH, Lee J, Ji BT, Lee YH, Young TL, Shin DH, Chun BY, Cho MC, Han BG, Hwu CM, Assimes TL, Absher D, Yan X, Kim E, Kuo JZ, Kwon S, Taylor KD, Chen YD, Rotter JI, Qi L, Zhu D, Wu T, Mohlke KL, Gu D, Mo Z, Wu JY, Lin X, Miki T, Tai ES, Lee JY, Kato N, Shu XO, Tanaka T
Human molecular genetics23(20)5492-504(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Significant associations were identified at 3.5 years for SEC16B rs10913469 with dietary parameters
Title: Validation of obesity susceptibility loci identified by genome-wide association studies in early childhood in South Brazilian children.

SEC16B, MC4R, MAP2K5 and KCTD15 (rs17782313, rs543874, rs2241423 and rs11084753) polymorphisms are associated with the risk for children obesity in China.
Title: Genetic variations in SEC16B, MC4R, MAP2K5 and KCTD15 were associated with childhood obesity and interacted with dietary behaviors in Chinese school-age population.

Data show the synthetic effect of SNPs on the indices of adiposity and risk of obesity in Chinese girls, but failed to replicate the effect of five separate variants of SEC16B rs10913469, SH2B1 rs4788102, PCSK1 rs6235, KCTD15 rs29941 and BAT2 rs2844479.
Title: Sex-dependent associations of genetic variants identified by GWAS with indices of adiposity and obesity risk in a Chinese children population.

Data show that knockdown of Sec16B but not Sec16A by RNAi affected the morphology of peroxisomes, inhibited the transport of Pex16 from the ER to peroxisomes, and suppressed expression of Pex3.
Title: Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells.

describe characterization of the localization and dynamics of Sec16B relative to Sec16A, provide evidence that Sec16B is likely a minor or perhaps specialized form of Sec16, and that it is not functionally redundant with S
Title: Characterization of human Sec16B: indications of specialized, non-redundant functions.

The following SEC16B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC16B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu103223	NM_001356499.2 Latest version!	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu103224	NM_001356500.1 Latest version!	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu14913	NM_033127.3 Latest version!	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$748.30 $1069.00
View Old Accession Versions >>
OHu14913	NM_033127.2	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$748.30 $1069.00
OHu103223	NM_001356499.1	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC16B ( NM_001356499.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001343428.1 Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu103223
Clone ID Related Accession (Same CDS sequence)	NM_001356499.1 , NM_001356499.2
Accession Version	NM_001356499.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	protein transport protein Sec16B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC009106.1, AL356279.8 and AL160007.10. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009106.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGAAG ATGTCTCCTC AGCTGGTCCC AAAGCACCCA TGAAGTTCTA CATCCCTCAT 
GTTCCTGTGA GTTTCGGGCC AGGAGGTCAG CTGGTGCATG TAGGTCCCAG CTCTCCCACT 
GACGGGCAAG CAGCCCTTGT TGAACTGCAC AGCATGGAGG TTATTCTTAA TGATTCCGAA 
GAGCAAGAGG AGATGAGAAG TTTCTCAGGA CCCTTGATTA GGGAAGATGT ACATAAGGTG 
GATATTATGA CGTTTTGCCA GCAGAAAGCA GCTCAGAGCT GCAAATCTGA GACACTGGGG 
AGCAGAGACT CAGCTCTACT GTGGCAGCTC TTGGTTCTCC TTTGTCGCCA GAATGGGTCC 
ATGGTGGGGT CTGACATCGC TGAGCTGCTA ATGCAAGACT GCAAGAAGCT GGAGAAGTAC 
AAGCGGCAGC CCCCTGTGGC CAACCTCATC AACCTGACCG ATGAGGACTG GCCAGTGCTG 
AGCTCTGGGA CCCCGAACCT GCTCACGGGA GAGATCCCCC CCAGTGTGGA GACACCTGCG 
CAGATCGTGG AGAAATTCAC TAGGCTGCTC TACTATGGAA GGAAGAAGGA AGCCTTGGAG 
TGGGCCATGA AGAACCACTT GTGGGGCCAT GCTTTGTTCC TGTCCAGCAA GATGGACCCA 
CAGACCTACA GCTGGGTCAT GAGTGGCTTC ACCAGCACGC TGGCGCTCAA TGACCCACTG 
CAGACCCTCT TCCAGCTCAT GTCGGGGAGG ATTCCACAGG CAGCCACGTG TTGTGGAGAA 
AAGCAGTGGG GAGACTGGAG GCCTCACTTG GCTGTGATTC TGTCGAATCA GGCTGGGGAC 
CCAGAGCTGT ATCAGCGTGC GATTGTTGCC ATTGGGGACA CCCTGGCTGG GAAGGGGCTT 
GTGGAGGCAG CTCACTTCTG CTATCTCATG GCTCACGTGC CCTTTGGCCA CTACACCGTG 
AAGACAGACC ATCTGGTCTT GCTGGGCAGC AGCCACAGGT ATGCGACCTG GGAGAAAGGA 
AACAGTAAGG ACATTTTCCA AGGGACTGTT TTAGCCCTTG TTGGGTTTTA TGGATCCATT 
TTTCATTTTC TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343428.1
CDS	83..1960
Translation	MELWAPQRLPQTRGKATAPSKDPDRGFRRDGHHRPVPHSWHNGERFHQWQDNRGSPQPQQEPRADHQQQPHYASRPGDWHQPVSGVDYYEGGYRNQLYSRPGYENSYQSYQSPTMREEYAYGSYYYHGHPQWLQEERVPRQRSPYIWHEDYREQKYLDEHHYENQHSPFGTNSETHFQSNSRNPCKDSPASNSGQEWPGELFPGSLLAEAQKNKPSLASESNLLQQRESGLSSSSYELSQYIRDAPERDDPPASAAWSPVQAEDVSSAGPKAPMKFYIPHVPVSFGPGGQLVHVGPSSPTDGQAALVELHSMEVILNDSEEQEEMRSFSGPLIREDVHKVDIMTFCQQKAAQSCKSETLGSRDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLSSGTPNLLTGEIPPSVETPAQIVEKFTRLLYYGRKKEALEWAMKNHLWGHALFLSSKMDPQTYSWVMSGFTSTLALNDPLQTLFQLMSGRIPQAATCCGEKQWGDWRPHLAVILSNQAGDPELYQRAIVAIGDTLAGKGLVEAAHFCYLMAHVPFGHYTVKTDHLVLLGSSHRYATWEKGNSKDIFQGTVLALVGFYGSIFHFLM

Target ORF information:

RefSeq Version	NM_001356499.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356499.1

ORF Insert Sequence:

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGAAG ATGTCTCCTC AGCTGGTCCC AAAGCACCCA TGAAGTTCTA CATCCCTCAT 
GTTCCTGTGA GTTTCGGGCC AGGAGGTCAG CTGGTGCATG TAGGTCCCAG CTCTCCCACT 
GACGGGCAAG CAGCCCTTGT TGAACTGCAC AGCATGGAGG TTATTCTTAA TGATTCCGAA 
GAGCAAGAGG AGATGAGAAG TTTCTCAGGA CCCTTGATTA GGGAAGATGT ACATAAGGTG 
GATATTATGA CGTTTTGCCA GCAGAAAGCA GCTCAGAGCT GCAAATCTGA GACACTGGGG 
AGCAGAGACT CAGCTCTACT GTGGCAGCTC TTGGTTCTCC TTTGTCGCCA GAATGGGTCC 
ATGGTGGGGT CTGACATCGC TGAGCTGCTA ATGCAAGACT GCAAGAAGCT GGAGAAGTAC 
AAGCGGCAGC CCCCTGTGGC CAACCTCATC AACCTGACCG ATGAGGACTG GCCAGTGCTG 
AGCTCTGGGA CCCCGAACCT GCTCACGGGA GAGATCCCCC CCAGTGTGGA GACACCTGCG 
CAGATCGTGG AGAAATTCAC TAGGCTGCTC TACTATGGAA GGAAGAAGGA AGCCTTGGAG 
TGGGCCATGA AGAACCACTT GTGGGGCCAT GCTTTGTTCC TGTCCAGCAA GATGGACCCA 
CAGACCTACA GCTGGGTCAT GAGTGGCTTC ACCAGCACGC TGGCGCTCAA TGACCCACTG 
CAGACCCTCT TCCAGCTCAT GTCGGGGAGG ATTCCACAGG CAGCCACGTG TTGTGGAGAA 
AAGCAGTGGG GAGACTGGAG GCCTCACTTG GCTGTGATTC TGTCGAATCA GGCTGGGGAC 
CCAGAGCTGT ATCAGCGTGC GATTGTTGCC ATTGGGGACA CCCTGGCTGG GAAGGGGCTT 
GTGGAGGCAG CTCACTTCTG CTATCTCATG GCTCACGTGC CCTTTGGCCA CTACACCGTG 
AAGACAGACC ATCTGGTCTT GCTGGGCAGC AGCCACAGGT ATGCGACCTG GGAGAAAGGA 
AACAGTAAGG ACATTTTCCA AGGGACTGTT TTAGCCCTTG TTGGGTTTTA TGGATCCATT 
TTTCATTTTC TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEC16B ( NM_001356499.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001343428.1 Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu103223
Clone ID Related Accession (Same CDS sequence)	NM_001356499.1 , NM_001356499.2
Accession Version	NM_001356499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	protein transport protein Sec16B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL356279.8, BC009106.1 and AL160007.10. On Jun 2, 2019 this sequence version replaced NM_001356499.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009106.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGAAG ATGTCTCCTC AGCTGGTCCC AAAGCACCCA TGAAGTTCTA CATCCCTCAT 
GTTCCTGTGA GTTTCGGGCC AGGAGGTCAG CTGGTGCATG TAGGTCCCAG CTCTCCCACT 
GACGGGCAAG CAGCCCTTGT TGAACTGCAC AGCATGGAGG TTATTCTTAA TGATTCCGAA 
GAGCAAGAGG AGATGAGAAG TTTCTCAGGA CCCTTGATTA GGGAAGATGT ACATAAGGTG 
GATATTATGA CGTTTTGCCA GCAGAAAGCA GCTCAGAGCT GCAAATCTGA GACACTGGGG 
AGCAGAGACT CAGCTCTACT GTGGCAGCTC TTGGTTCTCC TTTGTCGCCA GAATGGGTCC 
ATGGTGGGGT CTGACATCGC TGAGCTGCTA ATGCAAGACT GCAAGAAGCT GGAGAAGTAC 
AAGCGGCAGC CCCCTGTGGC CAACCTCATC AACCTGACCG ATGAGGACTG GCCAGTGCTG 
AGCTCTGGGA CCCCGAACCT GCTCACGGGA GAGATCCCCC CCAGTGTGGA GACACCTGCG 
CAGATCGTGG AGAAATTCAC TAGGCTGCTC TACTATGGAA GGAAGAAGGA AGCCTTGGAG 
TGGGCCATGA AGAACCACTT GTGGGGCCAT GCTTTGTTCC TGTCCAGCAA GATGGACCCA 
CAGACCTACA GCTGGGTCAT GAGTGGCTTC ACCAGCACGC TGGCGCTCAA TGACCCACTG 
CAGACCCTCT TCCAGCTCAT GTCGGGGAGG ATTCCACAGG CAGCCACGTG TTGTGGAGAA 
AAGCAGTGGG GAGACTGGAG GCCTCACTTG GCTGTGATTC TGTCGAATCA GGCTGGGGAC 
CCAGAGCTGT ATCAGCGTGC GATTGTTGCC ATTGGGGACA CCCTGGCTGG GAAGGGGCTT 
GTGGAGGCAG CTCACTTCTG CTATCTCATG GCTCACGTGC CCTTTGGCCA CTACACCGTG 
AAGACAGACC ATCTGGTCTT GCTGGGCAGC AGCCACAGGT ATGCGACCTG GGAGAAAGGA 
AACAGTAAGG ACATTTTCCA AGGGACTGTT TTAGCCCTTG TTGGGTTTTA TGGATCCATT 
TTTCATTTTC TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343428.1
CDS	114..1991
Translation	MELWAPQRLPQTRGKATAPSKDPDRGFRRDGHHRPVPHSWHNGERFHQWQDNRGSPQPQQEPRADHQQQPHYASRPGDWHQPVSGVDYYEGGYRNQLYSRPGYENSYQSYQSPTMREEYAYGSYYYHGHPQWLQEERVPRQRSPYIWHEDYREQKYLDEHHYENQHSPFGTNSETHFQSNSRNPCKDSPASNSGQEWPGELFPGSLLAEAQKNKPSLASESNLLQQRESGLSSSSYELSQYIRDAPERDDPPASAAWSPVQAEDVSSAGPKAPMKFYIPHVPVSFGPGGQLVHVGPSSPTDGQAALVELHSMEVILNDSEEQEEMRSFSGPLIREDVHKVDIMTFCQQKAAQSCKSETLGSRDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLSSGTPNLLTGEIPPSVETPAQIVEKFTRLLYYGRKKEALEWAMKNHLWGHALFLSSKMDPQTYSWVMSGFTSTLALNDPLQTLFQLMSGRIPQAATCCGEKQWGDWRPHLAVILSNQAGDPELYQRAIVAIGDTLAGKGLVEAAHFCYLMAHVPFGHYTVKTDHLVLLGSSHRYATWEKGNSKDIFQGTVLALVGFYGSIFHFLM

Target ORF information:

RefSeq Version	NM_001356499.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356499.2

ORF Insert Sequence:

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGAAG ATGTCTCCTC AGCTGGTCCC AAAGCACCCA TGAAGTTCTA CATCCCTCAT 
GTTCCTGTGA GTTTCGGGCC AGGAGGTCAG CTGGTGCATG TAGGTCCCAG CTCTCCCACT 
GACGGGCAAG CAGCCCTTGT TGAACTGCAC AGCATGGAGG TTATTCTTAA TGATTCCGAA 
GAGCAAGAGG AGATGAGAAG TTTCTCAGGA CCCTTGATTA GGGAAGATGT ACATAAGGTG 
GATATTATGA CGTTTTGCCA GCAGAAAGCA GCTCAGAGCT GCAAATCTGA GACACTGGGG 
AGCAGAGACT CAGCTCTACT GTGGCAGCTC TTGGTTCTCC TTTGTCGCCA GAATGGGTCC 
ATGGTGGGGT CTGACATCGC TGAGCTGCTA ATGCAAGACT GCAAGAAGCT GGAGAAGTAC 
AAGCGGCAGC CCCCTGTGGC CAACCTCATC AACCTGACCG ATGAGGACTG GCCAGTGCTG 
AGCTCTGGGA CCCCGAACCT GCTCACGGGA GAGATCCCCC CCAGTGTGGA GACACCTGCG 
CAGATCGTGG AGAAATTCAC TAGGCTGCTC TACTATGGAA GGAAGAAGGA AGCCTTGGAG 
TGGGCCATGA AGAACCACTT GTGGGGCCAT GCTTTGTTCC TGTCCAGCAA GATGGACCCA 
CAGACCTACA GCTGGGTCAT GAGTGGCTTC ACCAGCACGC TGGCGCTCAA TGACCCACTG 
CAGACCCTCT TCCAGCTCAT GTCGGGGAGG ATTCCACAGG CAGCCACGTG TTGTGGAGAA 
AAGCAGTGGG GAGACTGGAG GCCTCACTTG GCTGTGATTC TGTCGAATCA GGCTGGGGAC 
CCAGAGCTGT ATCAGCGTGC GATTGTTGCC ATTGGGGACA CCCTGGCTGG GAAGGGGCTT 
GTGGAGGCAG CTCACTTCTG CTATCTCATG GCTCACGTGC CCTTTGGCCA CTACACCGTG 
AAGACAGACC ATCTGGTCTT GCTGGGCAGC AGCCACAGGT ATGCGACCTG GGAGAAAGGA 
AACAGTAAGG ACATTTTCCA AGGGACTGTT TTAGCCCTTG TTGGGTTTTA TGGATCCATT 
TTTCATTTTC TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEC16B ( NM_001356500.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001343429.1 Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 3, mRNA.

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CloneID	OHu103224
Clone ID Related Accession (Same CDS sequence)	NM_001356500.1
Accession Version	NM_001356500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	protein transport protein Sec16B isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL359075.15, AL356279.8 and AL160007.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314585.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGTTTT GCCAGCAGAA AGCAGCTCAG AGCTGCAAAT CTGAGACACT GGGGAGCAGA 
GACTCAGCTC TACTGTGGCA GCTCTTGGTT CTCCTTTGTC GCCAGAATGG GTCCATGGTG 
GGGTCTGACA TCGCTGAGCT GCTAATGCAA GACTGCAAGA AGCTGGAGAA GTACAAGCGG 
CAGCCCCCTG TGGCCAACCT CATCAACCTG ACCGATGAGG ACTGGCCAGT GCTGAGCTCT 
GGGACCCCGA ACCTGCTCAC GGGAGAGATC CCCCCCAGTG TGGAGACACC TGCGCAGATC 
GTGGAGAAAT TCACTAGGCT GCTCTACTAT GGAAGGAAGA AGGAAGCCTT GGAGTGGGCC 
ATGAAGAACC ACTTGTGGGG CCATGCTTTG TTCCTGTCCA GCAAGATGGA CCCACAGACC 
TACAGCTGGG TCATGAGTGG CTTCACCAGC ACGCTGGCGC TCAATGACCC ACTGCAGACC 
CTCTTCCAGC TCATGTCGGG GAGGATTCCA CAGGCAGCCA CGTGTTGTGG AGAAAAGCAG 
TGGGGAGACT GGAGGCCTCA CTTGGCTGTG ATTCTGTCGA ATCAGGCTGG GGACCCAGAG 
CTGTATCAGC GTGCGATTGT TGCCATTGGG GACACCCTGG CTGGGAAGGG GCTTGTGGAG 
GCAGCTCACT TCTGCTATCT CATGGCTCAC GTGCCCTTTG GCCACTACAC CGTGAAGACA 
GACCATCTGG TCTTGCTGGG CAGCAGCCAC AGTCAAGAGT TTTTGAAATT TGCAACAACT 
GAGGCAATCC AGAGGACGGA AATCTTCGAG TACTGTCAGA TGCTGGGCCG CCCCAAATCC 
TTCATCCCTT CTTTCCAGGT GTATAAGCTC CTTTATGCTT CCCGTCTGGC AGATTATGGC 
CTCGTGTCCC AGGCTTTGCA TTACTGCGAA GCCATTGGTG CAGCTGTCTT GAGCCAGGGA 
GAGAGCAGTC ACCCTGTGCT ATTAGTGGAA CTCATCAAGC TAGCAGAGAA ACTGAAGCTG 
TCAGATCCTC TGGTTTTAGA AAGACGCAGT GGAGACAGGG ACCTGGAGCC AGATTGGCTA 
GCGCAACTTC GGAGGCAGCT GGAGCAAAAG GTAGCAGGAG ACATTGGGGA TCCTCATCCT 
ACTCGCTCAG ATATTTCGGG AGCCGGAGGA ACAACAACAG AAAACACTTT CTACCAGGAC 
TTTTCTGGAT GTCAAGGCTA CTCTGAAGCC CCTGGGTACC GCTCAGCTCT GTGGCTGACA 
CCTGAGCAGA CCTGCCTGCT CCAGCCCAGC CCACAGCAGC CCTTTCCCCT CCAGCCGGGC 
TCCTACCCAG CAGGAGGGGG TGCAGGGCAG ACAGGGACAC CGAGGCCTTT TTACTCAGTT 
CCTGAGACCC ATCTACCAGG GACTGGCAGC AGCGTGGCAG TGACAGAGGC CACTGGAGGA 
ACAGTCTGGG AGGAAATGCT GCAGACACAC CTGGGCCCTG GAGAGAACAC AGTGTCTCAA 
GAAACTTCCC AGCCTCCTGA TGGCCAAGAG GTCATTTCCA AACCACAGAC ACCATTGGCT 
GCTAGACCAC GAAGTATTTC TGAGAGTTCC GCCAGTTCAG CCAAGGAGGA TGAGAAGGAG 
TCCTCTGATG AGGCTGATAA AAACTCTCCC CGAAATACTG CCCAGAGAGG CAAGCTCGGA 
GATGGGAAGG AGCATACAAA GAGCTCAGGG TTTGGCTGGT TCAGCTGGTT TCGATCGAAG 
CCCACCAAGA ACGCATCCCC CGCTGGAGAC GAGGACTCCT CAGACAGCCC TGACTCTGAG 
GAGACCCCCA GAGCATCTTC TCCCCACCAG GCTGGCCTGG GCCTCTCACT GACACCTTCC 
CCTGAGTCCC CACCTCTGCC GGATGTTAGT GCCTTCTCCA GGGGCAGAGG TGGGGGTGAA 
GGCCGAGGAT CCGCATCCAG CGGGGGAGCA GCTGCGGGCG CTGGGGTTGG AGGCTTGTCT 
GGACCAGAGA GTGTTTCCTT TGAGCTCTGC TCCAACCCTG GTGTTCTTCT TCCTCCACCT 
GCCTTAAAAG GGGCTGTTCC TCTTTACAAC CCATCTCAGG TGCCTCAGCT GCCCACGGCC 
ACTAGCCTGA ATCGGCCAAA TCGCCTAGCT CAGCGTCGCT ATCCCACCCA GCCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343429.1
CDS	1118..3277
Translation	MTFCQQKAAQSCKSETLGSRDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLSSGTPNLLTGEIPPSVETPAQIVEKFTRLLYYGRKKEALEWAMKNHLWGHALFLSSKMDPQTYSWVMSGFTSTLALNDPLQTLFQLMSGRIPQAATCCGEKQWGDWRPHLAVILSNQAGDPELYQRAIVAIGDTLAGKGLVEAAHFCYLMAHVPFGHYTVKTDHLVLLGSSHSQEFLKFATTEAIQRTEIFEYCQMLGRPKSFIPSFQVYKLLYASRLADYGLVSQALHYCEAIGAAVLSQGESSHPVLLVELIKLAEKLKLSDPLVLERRSGDRDLEPDWLAQLRRQLEQKVAGDIGDPHPTRSDISGAGGTTTENTFYQDFSGCQGYSEAPGYRSALWLTPEQTCLLQPSPQQPFPLQPGSYPAGGGAGQTGTPRPFYSVPETHLPGTGSSVAVTEATGGTVWEEMLQTHLGPGENTVSQETSQPPDGQEVISKPQTPLAARPRSISESSASSAKEDEKESSDEADKNSPRNTAQRGKLGDGKEHTKSSGFGWFSWFRSKPTKNASPAGDEDSSDSPDSEETPRASSPHQAGLGLSLTPSPESPPLPDVSAFSRGRGGGEGRGSASSGGAAAGAGVGGLSGPESVSFELCSNPGVLLPPPALKGAVPLYNPSQVPQLPTATSLNRPNRLAQRRYPTQPC

Target ORF information:

RefSeq Version	NM_001356500.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356500.1

ORF Insert Sequence:

ATGACGTTTT GCCAGCAGAA AGCAGCTCAG AGCTGCAAAT CTGAGACACT GGGGAGCAGA 
GACTCAGCTC TACTGTGGCA GCTCTTGGTT CTCCTTTGTC GCCAGAATGG GTCCATGGTG 
GGGTCTGACA TCGCTGAGCT GCTAATGCAA GACTGCAAGA AGCTGGAGAA GTACAAGCGG 
CAGCCCCCTG TGGCCAACCT CATCAACCTG ACCGATGAGG ACTGGCCAGT GCTGAGCTCT 
GGGACCCCGA ACCTGCTCAC GGGAGAGATC CCCCCCAGTG TGGAGACACC TGCGCAGATC 
GTGGAGAAAT TCACTAGGCT GCTCTACTAT GGAAGGAAGA AGGAAGCCTT GGAGTGGGCC 
ATGAAGAACC ACTTGTGGGG CCATGCTTTG TTCCTGTCCA GCAAGATGGA CCCACAGACC 
TACAGCTGGG TCATGAGTGG CTTCACCAGC ACGCTGGCGC TCAATGACCC ACTGCAGACC 
CTCTTCCAGC TCATGTCGGG GAGGATTCCA CAGGCAGCCA CGTGTTGTGG AGAAAAGCAG 
TGGGGAGACT GGAGGCCTCA CTTGGCTGTG ATTCTGTCGA ATCAGGCTGG GGACCCAGAG 
CTGTATCAGC GTGCGATTGT TGCCATTGGG GACACCCTGG CTGGGAAGGG GCTTGTGGAG 
GCAGCTCACT TCTGCTATCT CATGGCTCAC GTGCCCTTTG GCCACTACAC CGTGAAGACA 
GACCATCTGG TCTTGCTGGG CAGCAGCCAC AGTCAAGAGT TTTTGAAATT TGCAACAACT 
GAGGCAATCC AGAGGACGGA AATCTTCGAG TACTGTCAGA TGCTGGGCCG CCCCAAATCC 
TTCATCCCTT CTTTCCAGGT GTATAAGCTC CTTTATGCTT CCCGTCTGGC AGATTATGGC 
CTCGTGTCCC AGGCTTTGCA TTACTGCGAA GCCATTGGTG CAGCTGTCTT GAGCCAGGGA 
GAGAGCAGTC ACCCTGTGCT ATTAGTGGAA CTCATCAAGC TAGCAGAGAA ACTGAAGCTG 
TCAGATCCTC TGGTTTTAGA AAGACGCAGT GGAGACAGGG ACCTGGAGCC AGATTGGCTA 
GCGCAACTTC GGAGGCAGCT GGAGCAAAAG GTAGCAGGAG ACATTGGGGA TCCTCATCCT 
ACTCGCTCAG ATATTTCGGG AGCCGGAGGA ACAACAACAG AAAACACTTT CTACCAGGAC 
TTTTCTGGAT GTCAAGGCTA CTCTGAAGCC CCTGGGTACC GCTCAGCTCT GTGGCTGACA 
CCTGAGCAGA CCTGCCTGCT CCAGCCCAGC CCACAGCAGC CCTTTCCCCT CCAGCCGGGC 
TCCTACCCAG CAGGAGGGGG TGCAGGGCAG ACAGGGACAC CGAGGCCTTT TTACTCAGTT 
CCTGAGACCC ATCTACCAGG GACTGGCAGC AGCGTGGCAG TGACAGAGGC CACTGGAGGA 
ACAGTCTGGG AGGAAATGCT GCAGACACAC CTGGGCCCTG GAGAGAACAC AGTGTCTCAA 
GAAACTTCCC AGCCTCCTGA TGGCCAAGAG GTCATTTCCA AACCACAGAC ACCATTGGCT 
GCTAGACCAC GAAGTATTTC TGAGAGTTCC GCCAGTTCAG CCAAGGAGGA TGAGAAGGAG 
TCCTCTGATG AGGCTGATAA AAACTCTCCC CGAAATACTG CCCAGAGAGG CAAGCTCGGA 
GATGGGAAGG AGCATACAAA GAGCTCAGGG TTTGGCTGGT TCAGCTGGTT TCGATCGAAG 
CCCACCAAGA ACGCATCCCC CGCTGGAGAC GAGGACTCCT CAGACAGCCC TGACTCTGAG 
GAGACCCCCA GAGCATCTTC TCCCCACCAG GCTGGCCTGG GCCTCTCACT GACACCTTCC 
CCTGAGTCCC CACCTCTGCC GGATGTTAGT GCCTTCTCCA GGGGCAGAGG TGGGGGTGAA 
GGCCGAGGAT CCGCATCCAG CGGGGGAGCA GCTGCGGGCG CTGGGGTTGG AGGCTTGTCT 
GGACCAGAGA GTGTTTCCTT TGAGCTCTGC TCCAACCCTG GTGTTCTTCT TCCTCCACCT 
GCCTTAAAAG GGGCTGTTCC TCTTTACAAC CCATCTCAGG TGCCTCAGCT GCCCACGGCC 
ACTAGCCTGA ATCGGCCAAA TCGCCTAGCT CAGCGTCGCT ATCCCACCCA GCCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEC16B ( NM_033127.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_149118.2 Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), mRNA.

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CloneID	OHu14913
Clone ID Related Accession (Same CDS sequence)	NM_033127.2 , NM_033127.3
Accession Version	NM_033127.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-27
Organism	Homo sapiens(Human)
Product	protein transport protein Sec16B
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB063357.1, AK098627.1, AV697113.1 and AK090971.1. On Oct 17, 2006 this sequence version replaced NM_033127.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB063357.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGATG TCTCCTCAGC TGGTCCCAAA GCACCCATGA AGTTCTACAT CCCTCATGTT 
CCTGTGAGTT TCGGGCCAGG AGGTCAGCTG GTGCATGTAG GTCCCAGCTC TCCCACTGAC 
GGGCAAGCAG CCCTTGTTGA ACTGCACAGC ATGGAGGTTA TTCTTAATGA TTCCGAAGAG 
CAAGAGGAGA TGAGAAGTTT CTCAGGACCC TTGATTAGGG AAGATGTACA TAAGGTGGAT 
ATTATGACGT TTTGCCAGCA GAAAGCAGCT CAGAGCTGCA AATCTGAGAC ACTGGGGAGC 
AGAGACTCAG CTCTACTGTG GCAGCTCTTG GTTCTCCTTT GTCGCCAGAA TGGGTCCATG 
GTGGGGTCTG ACATCGCTGA GCTGCTAATG CAAGACTGCA AGAAGCTGGA GAAGTACAAG 
CGGCAGCCCC CTGTGGCCAA CCTCATCAAC CTGACCGATG AGGACTGGCC AGTGCTGAGC 
TCTGGGACCC CGAACCTGCT CACGGGAGAG ATCCCCCCCA GTGTGGAGAC ACCTGCGCAG 
ATCGTGGAGA AATTCACTAG GCTGCTCTAC TATGGAAGGA AGAAGGAAGC CTTGGAGTGG 
GCCATGAAGA ACCACTTGTG GGGCCATGCT TTGTTCCTGT CCAGCAAGAT GGACCCACAG 
ACCTACAGCT GGGTCATGAG TGGCTTCACC AGCACGCTGG CGCTCAATGA CCCACTGCAG 
ACCCTCTTCC AGCTCATGTC GGGGAGGATT CCACAGGCAG CCACGTGTTG TGGAGAAAAG 
CAGTGGGGAG ACTGGAGGCC TCACTTGGCT GTGATTCTGT CGAATCAGGC TGGGGACCCA 
GAGCTGTATC AGCGTGCGAT TGTTGCCATT GGGGACACCC TGGCTGGGAA GGGGCTTGTG 
GAGGCAGCTC ACTTCTGCTA TCTCATGGCT CACGTGCCCT TTGGCCACTA CACCGTGAAG 
ACAGACCATC TGGTCTTGCT GGGCAGCAGC CACAGTCAAG AGTTTTTGAA ATTTGCAACA 
ACTGAGGCAA TCCAGAGGAC GGAAATCTTC GAGTACTGTC AGATGCTGGG CCGCCCCAAA 
TCCTTCATCC CTTCTTTCCA GGTGTATAAG CTCCTTTATG CTTCCCGTCT GGCAGATTAT 
GGCCTCGTGT CCCAGGCTTT GCATTACTGC GAAGCCATTG GTGCAGCTGT CTTGAGCCAG 
GGAGAGAGCA GTCACCCTGT GCTATTAGTG GAACTCATCA AGCTAGCAGA GAAACTGAAG 
CTGTCAGATC CTCTGGTTTT AGAAAGACGC AGTGGAGACA GGGACCTGGA GCCAGATTGG 
CTAGCGCAAC TTCGGAGGCA GCTGGAGCAA AAGGTAGCAG GAGACATTGG GGATCCTCAT 
CCTACTCGCT CAGATATTTC GGGAGCCGGA GGAACAACAA CAGAAAACAC TTTCTACCAG 
GACTTTTCTG GATGTCAAGG CTACTCTGAA GCCCCTGGGT ACCGCTCAGC TCTGTGGCTG 
ACACCTGAGC AGACCTGCCT GCTCCAGCCC AGCCCACAGC AGCCCTTTCC CCTCCAGCCG 
GGCTCCTACC CAGCAGGAGG GGGTGCAGGG CAGACAGGGA CACCGAGGCC TTTTTACTCA 
GTTCCTGAGA CCCATCTACC AGGGACTGGC AGCAGCGTGG CAGTGACAGA GGCCACTGGA 
GGAACAGTCT GGGAGGAAAT GCTGCAGACA CACCTGGGCC CTGGAGAGAA CACAGTGTCT 
CAAGAAACTT CCCAGCCTCC TGATGGCCAA GAGGTCATTT CCAAACCACA GACACCATTG 
GCTGCTAGAC CACGAAGTAT TTCTGAGAGT TCCGCCAGTT CAGCCAAGGA GGATGAGAAG 
GAGTCCTCTG ATGAGGCTGA TAAAAACTCT CCCCGAAATA CTGCCCAGAG AGGCAAGCTC 
GGAGATGGGA AGGAGCATAC AAAGAGCTCA GGGTTTGGCT GGTTCAGCTG GTTTCGATCG 
AAGCCCACCA AGAACGCATC CCCCGCTGGA GACGAGGACT CCTCAGACAG CCCTGACTCT 
GAGGAGACCC CCAGAGCATC TTCTCCCCAC CAGGCTGGCC TGGGCCTCTC ACTGACACCT 
TCCCCTGAGT CCCCACCTCT GCCGGATGTT AGTGCCTTCT CCAGGGGCAG AGGTGGGGGT 
GAAGGCCGAG GATCCGCATC CAGCGGGGGA GCAGCTGCGG GCGCTGGGGT TGGAGGCTTG 
TCTGGACCAG AGAGTGTTTC CTTTGAGCTC TGCTCCAACC CTGGTGTTCT TCTTCCTCCA 
CCTGCCTTAA AAGGGGCTGT TCCTCTTTAC AACCCATCTC AGGTGCCTCA GCTGCCCACG 
GCCACTAGCC TGAATCGGCC AAATCGCCTA GCTCAGCGTC GCTATCCCAC CCAGCCATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149118.2
CDS	91..3273
Translation	MELWAPQRLPQTRGKATAPSKDPDRGFRRDGHHRPVPHSWHNGERFHQWQDNRGSPQPQQEPRADHQQQPHYASRPGDWHQPVSGVDYYEGGYRNQLYSRPGYENSYQSYQSPTMREEYAYGSYYYHGHPQWLQEERVPRQRSPYIWHEDYREQKYLDEHHYENQHSPFGTNSETHFQSNSRNPCKDSPASNSGQEWPGELFPGSLLAEAQKNKPSLASESNLLQQRESGLSSSSYELSQYIRDAPERDDPPASAAWSPVQADVSSAGPKAPMKFYIPHVPVSFGPGGQLVHVGPSSPTDGQAALVELHSMEVILNDSEEQEEMRSFSGPLIREDVHKVDIMTFCQQKAAQSCKSETLGSRDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLSSGTPNLLTGEIPPSVETPAQIVEKFTRLLYYGRKKEALEWAMKNHLWGHALFLSSKMDPQTYSWVMSGFTSTLALNDPLQTLFQLMSGRIPQAATCCGEKQWGDWRPHLAVILSNQAGDPELYQRAIVAIGDTLAGKGLVEAAHFCYLMAHVPFGHYTVKTDHLVLLGSSHSQEFLKFATTEAIQRTEIFEYCQMLGRPKSFIPSFQVYKLLYASRLADYGLVSQALHYCEAIGAAVLSQGESSHPVLLVELIKLAEKLKLSDPLVLERRSGDRDLEPDWLAQLRRQLEQKVAGDIGDPHPTRSDISGAGGTTTENTFYQDFSGCQGYSEAPGYRSALWLTPEQTCLLQPSPQQPFPLQPGSYPAGGGAGQTGTPRPFYSVPETHLPGTGSSVAVTEATGGTVWEEMLQTHLGPGENTVSQETSQPPDGQEVISKPQTPLAARPRSISESSASSAKEDEKESSDEADKNSPRNTAQRGKLGDGKEHTKSSGFGWFSWFRSKPTKNASPAGDEDSSDSPDSEETPRASSPHQAGLGLSLTPSPESPPLPDVSAFSRGRGGGEGRGSASSGGAAAGAGVGGLSGPESVSFELCSNPGVLLPPPALKGAVPLYNPSQVPQLPTATSLNRPNRLAQRRYPTQPC

Target ORF information:

RefSeq Version	NM_033127.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033127.2

ORF Insert Sequence:

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGATG TCTCCTCAGC TGGTCCCAAA GCACCCATGA AGTTCTACAT CCCTCATGTT 
CCTGTGAGTT TCGGGCCAGG AGGTCAGCTG GTGCATGTAG GTCCCAGCTC TCCCACTGAC 
GGGCAAGCAG CCCTTGTTGA ACTGCACAGC ATGGAGGTTA TTCTTAATGA TTCCGAAGAG 
CAAGAGGAGA TGAGAAGTTT CTCAGGACCC TTGATTAGGG AAGATGTACA TAAGGTGGAT 
ATTATGACGT TTTGCCAGCA GAAAGCAGCT CAGAGCTGCA AATCTGAGAC ACTGGGGAGC 
AGAGACTCAG CTCTACTGTG GCAGCTCTTG GTTCTCCTTT GTCGCCAGAA TGGGTCCATG 
GTGGGGTCTG ACATCGCTGA GCTGCTAATG CAAGACTGCA AGAAGCTGGA GAAGTACAAG 
CGGCAGCCCC CTGTGGCCAA CCTCATCAAC CTGACCGATG AGGACTGGCC AGTGCTGAGC 
TCTGGGACCC CGAACCTGCT CACGGGAGAG ATCCCCCCCA GTGTGGAGAC ACCTGCGCAG 
ATCGTGGAGA AATTCACTAG GCTGCTCTAC TATGGAAGGA AGAAGGAAGC CTTGGAGTGG 
GCCATGAAGA ACCACTTGTG GGGCCATGCT TTGTTCCTGT CCAGCAAGAT GGACCCACAG 
ACCTACAGCT GGGTCATGAG TGGCTTCACC AGCACGCTGG CGCTCAATGA CCCACTGCAG 
ACCCTCTTCC AGCTCATGTC GGGGAGGATT CCACAGGCAG CCACGTGTTG TGGAGAAAAG 
CAGTGGGGAG ACTGGAGGCC TCACTTGGCT GTGATTCTGT CGAATCAGGC TGGGGACCCA 
GAGCTGTATC AGCGTGCGAT TGTTGCCATT GGGGACACCC TGGCTGGGAA GGGGCTTGTG 
GAGGCAGCTC ACTTCTGCTA TCTCATGGCT CACGTGCCCT TTGGCCACTA CACCGTGAAG 
ACAGACCATC TGGTCTTGCT GGGCAGCAGC CACAGTCAAG AGTTTTTGAA ATTTGCAACA 
ACTGAGGCAA TCCAGAGGAC GGAAATCTTC GAGTACTGTC AGATGCTGGG CCGCCCCAAA 
TCCTTCATCC CTTCTTTCCA GGTGTATAAG CTCCTTTATG CTTCCCGTCT GGCAGATTAT 
GGCCTCGTGT CCCAGGCTTT GCATTACTGC GAAGCCATTG GTGCAGCTGT CTTGAGCCAG 
GGAGAGAGCA GTCACCCTGT GCTATTAGTG GAACTCATCA AGCTAGCAGA GAAACTGAAG 
CTGTCAGATC CTCTGGTTTT AGAAAGACGC AGTGGAGACA GGGACCTGGA GCCAGATTGG 
CTAGCGCAAC TTCGGAGGCA GCTGGAGCAA AAGGTAGCAG GAGACATTGG GGATCCTCAT 
CCTACTCGCT CAGATATTTC GGGAGCCGGA GGAACAACAA CAGAAAACAC TTTCTACCAG 
GACTTTTCTG GATGTCAAGG CTACTCTGAA GCCCCTGGGT ACCGCTCAGC TCTGTGGCTG 
ACACCTGAGC AGACCTGCCT GCTCCAGCCC AGCCCACAGC AGCCCTTTCC CCTCCAGCCG 
GGCTCCTACC CAGCAGGAGG GGGTGCAGGG CAGACAGGGA CACCGAGGCC TTTTTACTCA 
GTTCCTGAGA CCCATCTACC AGGGACTGGC AGCAGCGTGG CAGTGACAGA GGCCACTGGA 
GGAACAGTCT GGGAGGAAAT GCTGCAGACA CACCTGGGCC CTGGAGAGAA CACAGTGTCT 
CAAGAAACTT CCCAGCCTCC TGATGGCCAA GAGGTCATTT CCAAACCACA GACACCATTG 
GCTGCTAGAC CACGAAGTAT TTCTGAGAGT TCCGCCAGTT CAGCCAAGGA GGATGAGAAG 
GAGTCCTCTG ATGAGGCTGA TAAAAACTCT CCCCGAAATA CTGCCCAGAG AGGCAAGCTC 
GGAGATGGGA AGGAGCATAC AAAGAGCTCA GGGTTTGGCT GGTTCAGCTG GTTTCGATCG 
AAGCCCACCA AGAACGCATC CCCCGCTGGA GACGAGGACT CCTCAGACAG CCCTGACTCT 
GAGGAGACCC CCAGAGCATC TTCTCCCCAC CAGGCTGGCC TGGGCCTCTC ACTGACACCT 
TCCCCTGAGT CCCCACCTCT GCCGGATGTT AGTGCCTTCT CCAGGGGCAG AGGTGGGGGT 
GAAGGCCGAG GATCCGCATC CAGCGGGGGA GCAGCTGCGG GCGCTGGGGT TGGAGGCTTG 
TCTGGACCAG AGAGTGTTTC CTTTGAGCTC TGCTCCAACC CTGGTGTTCT TCTTCCTCCA 
CCTGCCTTAA AAGGGGCTGT TCCTCTTTAC AACCCATCTC AGGTGCCTCA GCTGCCCACG 
GCCACTAGCC TGAATCGGCC AAATCGCCTA GCTCAGCGTC GCTATCCCAC CCAGCCATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEC16B ( NM_033127.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_149118.2 Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 1, mRNA.

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CloneID	OHu14913
Clone ID Related Accession (Same CDS sequence)	NM_033127.2 , NM_033127.3
Accession Version	NM_033127.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	protein transport protein Sec16B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX481418.1, AB063357.1, AK098627.1, AV697113.1, AK090971.1 and AA086201.1. On Oct 5, 2017 this sequence version replaced NM_033127.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB063357.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGATG TCTCCTCAGC TGGTCCCAAA GCACCCATGA AGTTCTACAT CCCTCATGTT 
CCTGTGAGTT TCGGGCCAGG AGGTCAGCTG GTGCATGTAG GTCCCAGCTC TCCCACTGAC 
GGGCAAGCAG CCCTTGTTGA ACTGCACAGC ATGGAGGTTA TTCTTAATGA TTCCGAAGAG 
CAAGAGGAGA TGAGAAGTTT CTCAGGACCC TTGATTAGGG AAGATGTACA TAAGGTGGAT 
ATTATGACGT TTTGCCAGCA GAAAGCAGCT CAGAGCTGCA AATCTGAGAC ACTGGGGAGC 
AGAGACTCAG CTCTACTGTG GCAGCTCTTG GTTCTCCTTT GTCGCCAGAA TGGGTCCATG 
GTGGGGTCTG ACATCGCTGA GCTGCTAATG CAAGACTGCA AGAAGCTGGA GAAGTACAAG 
CGGCAGCCCC CTGTGGCCAA CCTCATCAAC CTGACCGATG AGGACTGGCC AGTGCTGAGC 
TCTGGGACCC CGAACCTGCT CACGGGAGAG ATCCCCCCCA GTGTGGAGAC ACCTGCGCAG 
ATCGTGGAGA AATTCACTAG GCTGCTCTAC TATGGAAGGA AGAAGGAAGC CTTGGAGTGG 
GCCATGAAGA ACCACTTGTG GGGCCATGCT TTGTTCCTGT CCAGCAAGAT GGACCCACAG 
ACCTACAGCT GGGTCATGAG TGGCTTCACC AGCACGCTGG CGCTCAATGA CCCACTGCAG 
ACCCTCTTCC AGCTCATGTC GGGGAGGATT CCACAGGCAG CCACGTGTTG TGGAGAAAAG 
CAGTGGGGAG ACTGGAGGCC TCACTTGGCT GTGATTCTGT CGAATCAGGC TGGGGACCCA 
GAGCTGTATC AGCGTGCGAT TGTTGCCATT GGGGACACCC TGGCTGGGAA GGGGCTTGTG 
GAGGCAGCTC ACTTCTGCTA TCTCATGGCT CACGTGCCCT TTGGCCACTA CACCGTGAAG 
ACAGACCATC TGGTCTTGCT GGGCAGCAGC CACAGTCAAG AGTTTTTGAA ATTTGCAACA 
ACTGAGGCAA TCCAGAGGAC GGAAATCTTC GAGTACTGTC AGATGCTGGG CCGCCCCAAA 
TCCTTCATCC CTTCTTTCCA GGTGTATAAG CTCCTTTATG CTTCCCGTCT GGCAGATTAT 
GGCCTCGTGT CCCAGGCTTT GCATTACTGC GAAGCCATTG GTGCAGCTGT CTTGAGCCAG 
GGAGAGAGCA GTCACCCTGT GCTATTAGTG GAACTCATCA AGCTAGCAGA GAAACTGAAG 
CTGTCAGATC CTCTGGTTTT AGAAAGACGC AGTGGAGACA GGGACCTGGA GCCAGATTGG 
CTAGCGCAAC TTCGGAGGCA GCTGGAGCAA AAGGTAGCAG GAGACATTGG GGATCCTCAT 
CCTACTCGCT CAGATATTTC GGGAGCCGGA GGAACAACAA CAGAAAACAC TTTCTACCAG 
GACTTTTCTG GATGTCAAGG CTACTCTGAA GCCCCTGGGT ACCGCTCAGC TCTGTGGCTG 
ACACCTGAGC AGACCTGCCT GCTCCAGCCC AGCCCACAGC AGCCCTTTCC CCTCCAGCCG 
GGCTCCTACC CAGCAGGAGG GGGTGCAGGG CAGACAGGGA CACCGAGGCC TTTTTACTCA 
GTTCCTGAGA CCCATCTACC AGGGACTGGC AGCAGCGTGG CAGTGACAGA GGCCACTGGA 
GGAACAGTCT GGGAGGAAAT GCTGCAGACA CACCTGGGCC CTGGAGAGAA CACAGTGTCT 
CAAGAAACTT CCCAGCCTCC TGATGGCCAA GAGGTCATTT CCAAACCACA GACACCATTG 
GCTGCTAGAC CACGAAGTAT TTCTGAGAGT TCCGCCAGTT CAGCCAAGGA GGATGAGAAG 
GAGTCCTCTG ATGAGGCTGA TAAAAACTCT CCCCGAAATA CTGCCCAGAG AGGCAAGCTC 
GGAGATGGGA AGGAGCATAC AAAGAGCTCA GGGTTTGGCT GGTTCAGCTG GTTTCGATCG 
AAGCCCACCA AGAACGCATC CCCCGCTGGA GACGAGGACT CCTCAGACAG CCCTGACTCT 
GAGGAGACCC CCAGAGCATC TTCTCCCCAC CAGGCTGGCC TGGGCCTCTC ACTGACACCT 
TCCCCTGAGT CCCCACCTCT GCCGGATGTT AGTGCCTTCT CCAGGGGCAG AGGTGGGGGT 
GAAGGCCGAG GATCCGCATC CAGCGGGGGA GCAGCTGCGG GCGCTGGGGT TGGAGGCTTG 
TCTGGACCAG AGAGTGTTTC CTTTGAGCTC TGCTCCAACC CTGGTGTTCT TCTTCCTCCA 
CCTGCCTTAA AAGGGGCTGT TCCTCTTTAC AACCCATCTC AGGTGCCTCA GCTGCCCACG 
GCCACTAGCC TGAATCGGCC AAATCGCCTA GCTCAGCGTC GCTATCCCAC CCAGCCATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149118.2
CDS	98..3280
Translation	MELWAPQRLPQTRGKATAPSKDPDRGFRRDGHHRPVPHSWHNGERFHQWQDNRGSPQPQQEPRADHQQQPHYASRPGDWHQPVSGVDYYEGGYRNQLYSRPGYENSYQSYQSPTMREEYAYGSYYYHGHPQWLQEERVPRQRSPYIWHEDYREQKYLDEHHYENQHSPFGTNSETHFQSNSRNPCKDSPASNSGQEWPGELFPGSLLAEAQKNKPSLASESNLLQQRESGLSSSSYELSQYIRDAPERDDPPASAAWSPVQADVSSAGPKAPMKFYIPHVPVSFGPGGQLVHVGPSSPTDGQAALVELHSMEVILNDSEEQEEMRSFSGPLIREDVHKVDIMTFCQQKAAQSCKSETLGSRDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLSSGTPNLLTGEIPPSVETPAQIVEKFTRLLYYGRKKEALEWAMKNHLWGHALFLSSKMDPQTYSWVMSGFTSTLALNDPLQTLFQLMSGRIPQAATCCGEKQWGDWRPHLAVILSNQAGDPELYQRAIVAIGDTLAGKGLVEAAHFCYLMAHVPFGHYTVKTDHLVLLGSSHSQEFLKFATTEAIQRTEIFEYCQMLGRPKSFIPSFQVYKLLYASRLADYGLVSQALHYCEAIGAAVLSQGESSHPVLLVELIKLAEKLKLSDPLVLERRSGDRDLEPDWLAQLRRQLEQKVAGDIGDPHPTRSDISGAGGTTTENTFYQDFSGCQGYSEAPGYRSALWLTPEQTCLLQPSPQQPFPLQPGSYPAGGGAGQTGTPRPFYSVPETHLPGTGSSVAVTEATGGTVWEEMLQTHLGPGENTVSQETSQPPDGQEVISKPQTPLAARPRSISESSASSAKEDEKESSDEADKNSPRNTAQRGKLGDGKEHTKSSGFGWFSWFRSKPTKNASPAGDEDSSDSPDSEETPRASSPHQAGLGLSLTPSPESPPLPDVSAFSRGRGGGEGRGSASSGGAAAGAGVGGLSGPESVSFELCSNPGVLLPPPALKGAVPLYNPSQVPQLPTATSLNRPNRLAQRRYPTQPC

Target ORF information:

RefSeq Version	NM_033127.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033127.3

ORF Insert Sequence:

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGATG TCTCCTCAGC TGGTCCCAAA GCACCCATGA AGTTCTACAT CCCTCATGTT 
CCTGTGAGTT TCGGGCCAGG AGGTCAGCTG GTGCATGTAG GTCCCAGCTC TCCCACTGAC 
GGGCAAGCAG CCCTTGTTGA ACTGCACAGC ATGGAGGTTA TTCTTAATGA TTCCGAAGAG 
CAAGAGGAGA TGAGAAGTTT CTCAGGACCC TTGATTAGGG AAGATGTACA TAAGGTGGAT 
ATTATGACGT TTTGCCAGCA GAAAGCAGCT CAGAGCTGCA AATCTGAGAC ACTGGGGAGC 
AGAGACTCAG CTCTACTGTG GCAGCTCTTG GTTCTCCTTT GTCGCCAGAA TGGGTCCATG 
GTGGGGTCTG ACATCGCTGA GCTGCTAATG CAAGACTGCA AGAAGCTGGA GAAGTACAAG 
CGGCAGCCCC CTGTGGCCAA CCTCATCAAC CTGACCGATG AGGACTGGCC AGTGCTGAGC 
TCTGGGACCC CGAACCTGCT CACGGGAGAG ATCCCCCCCA GTGTGGAGAC ACCTGCGCAG 
ATCGTGGAGA AATTCACTAG GCTGCTCTAC TATGGAAGGA AGAAGGAAGC CTTGGAGTGG 
GCCATGAAGA ACCACTTGTG GGGCCATGCT TTGTTCCTGT CCAGCAAGAT GGACCCACAG 
ACCTACAGCT GGGTCATGAG TGGCTTCACC AGCACGCTGG CGCTCAATGA CCCACTGCAG 
ACCCTCTTCC AGCTCATGTC GGGGAGGATT CCACAGGCAG CCACGTGTTG TGGAGAAAAG 
CAGTGGGGAG ACTGGAGGCC TCACTTGGCT GTGATTCTGT CGAATCAGGC TGGGGACCCA 
GAGCTGTATC AGCGTGCGAT TGTTGCCATT GGGGACACCC TGGCTGGGAA GGGGCTTGTG 
GAGGCAGCTC ACTTCTGCTA TCTCATGGCT CACGTGCCCT TTGGCCACTA CACCGTGAAG 
ACAGACCATC TGGTCTTGCT GGGCAGCAGC CACAGTCAAG AGTTTTTGAA ATTTGCAACA 
ACTGAGGCAA TCCAGAGGAC GGAAATCTTC GAGTACTGTC AGATGCTGGG CCGCCCCAAA 
TCCTTCATCC CTTCTTTCCA GGTGTATAAG CTCCTTTATG CTTCCCGTCT GGCAGATTAT 
GGCCTCGTGT CCCAGGCTTT GCATTACTGC GAAGCCATTG GTGCAGCTGT CTTGAGCCAG 
GGAGAGAGCA GTCACCCTGT GCTATTAGTG GAACTCATCA AGCTAGCAGA GAAACTGAAG 
CTGTCAGATC CTCTGGTTTT AGAAAGACGC AGTGGAGACA GGGACCTGGA GCCAGATTGG 
CTAGCGCAAC TTCGGAGGCA GCTGGAGCAA AAGGTAGCAG GAGACATTGG GGATCCTCAT 
CCTACTCGCT CAGATATTTC GGGAGCCGGA GGAACAACAA CAGAAAACAC TTTCTACCAG 
GACTTTTCTG GATGTCAAGG CTACTCTGAA GCCCCTGGGT ACCGCTCAGC TCTGTGGCTG 
ACACCTGAGC AGACCTGCCT GCTCCAGCCC AGCCCACAGC AGCCCTTTCC CCTCCAGCCG 
GGCTCCTACC CAGCAGGAGG GGGTGCAGGG CAGACAGGGA CACCGAGGCC TTTTTACTCA 
GTTCCTGAGA CCCATCTACC AGGGACTGGC AGCAGCGTGG CAGTGACAGA GGCCACTGGA 
GGAACAGTCT GGGAGGAAAT GCTGCAGACA CACCTGGGCC CTGGAGAGAA CACAGTGTCT 
CAAGAAACTT CCCAGCCTCC TGATGGCCAA GAGGTCATTT CCAAACCACA GACACCATTG 
GCTGCTAGAC CACGAAGTAT TTCTGAGAGT TCCGCCAGTT CAGCCAAGGA GGATGAGAAG 
GAGTCCTCTG ATGAGGCTGA TAAAAACTCT CCCCGAAATA CTGCCCAGAG AGGCAAGCTC 
GGAGATGGGA AGGAGCATAC AAAGAGCTCA GGGTTTGGCT GGTTCAGCTG GTTTCGATCG 
AAGCCCACCA AGAACGCATC CCCCGCTGGA GACGAGGACT CCTCAGACAG CCCTGACTCT 
GAGGAGACCC CCAGAGCATC TTCTCCCCAC CAGGCTGGCC TGGGCCTCTC ACTGACACCT 
TCCCCTGAGT CCCCACCTCT GCCGGATGTT AGTGCCTTCT CCAGGGGCAG AGGTGGGGGT 
GAAGGCCGAG GATCCGCATC CAGCGGGGGA GCAGCTGCGG GCGCTGGGGT TGGAGGCTTG 
TCTGGACCAG AGAGTGTTTC CTTTGAGCTC TGCTCCAACC CTGGTGTTCT TCTTCCTCCA 
CCTGCCTTAA AAGGGGCTGT TCCTCTTTAC AACCCATCTC AGGTGCCTCA GCTGCCCACG 
GCCACTAGCC TGAATCGGCC AAATCGCCTA GCTCAGCGTC GCTATCCCAC CCAGCCATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Validation of obesity susceptibility loci identified by genome-wide association studies in early childhood in South Brazilian children.
Pediatric obesity12(1)85-92(2017 Feb)
Zandon? MR,Sangalli CN,Campagnolo PD,Vitolo MR,Almeida S,Mattevi VS

Genetic variations in SEC16B, MC4R, MAP2K5 and KCTD15 were associated with childhood obesity and interacted with dietary behaviors in Chinese school-age population.
Gene560(2)149-55(2015 Apr)
Lv D,Zhang DD,Wang H,Zhang Y,Liang L,Fu JF,Xiong F,Liu GL,Gong CX,Luo FH,Chen SK,Li ZL,Zhu YM

Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche.
Nature514(7520)92-97(2014 Oct)
Perry JR,Day F,Elks CE,Sulem P,Thompson DJ,Ferreira T,He C,Chasman DI,Esko T,Thorleifsson G,Albrecht E,Ang WQ,Corre T,Cousminer DL,Feenstra B,Franceschini N,Ganna A,Johnson AD,Kjellqvist S,Lunetta KL,McMahon G,Nolte IM,Paternoster L,Porcu E,Smith AV,Stolk L,Teumer A,T?ernikova N,Tikkanen E,Ulivi S,Wagner EK,Amin N,Bierut LJ,Byrne EM,Hottenga JJ,Koller DL,Mangino M,Pers TH,Yerges-Armstrong LM,Zhao JH,Andrulis IL,Anton-Culver H,Atsma F,Bandinelli S,Beckmann MW,Benitez J,Blomqvist C,Bojesen SE,Bolla MK,Bonanni B,Brauch H,Brenner H,Buring JE,Chang-Claude J,Chanock S,Chen J,Chenevix-Trench G,Coll?e JM,Couch FJ,Couper D,Coveillo AD,Cox A,Czene K,D'adamo AP,Smith GD,De Vivo I,Demerath EW,Dennis J,Devilee P,Dieffenbach AK,Dunning AM,Eiriksdottir G,Eriksson JG,Fasching PA,Ferrucci L,Flesch-Janys D,Flyger H,Foroud T,Franke L,Garcia ME,Garc?a-Closas M,Geller F,de Geus EE,Giles GG,Gudbjartsson DF,Gudnason V,Gu?nel P,Guo S,Hall P,Hamann U,Haring R,Hartman CA,Heath AC,Hofman A,Hooning MJ,Hopper JL,Hu FB,Hunter DJ,Karasik D,Kiel DP,Knight JA,Kosma VM,Kutalik Z,Lai S,Lambrechts D,Lindblom A,M?gi R,Magnusson PK,Mannermaa A,Martin NG,Masson G,McArdle PF,McArdle WL,Melbye M,Michailidou K,Mihailov E,Milani L,Milne RL,Nevanlinna H,Neven P,Nohr EA,Oldehinkel AJ,Oostra BA,Palotie A,Peacock M,Pedersen NL,Peterlongo P,Peto J,Pharoah PD,Postma DS,Pouta A,Pylk?s K,Radice P,Ring S,Rivadeneira F,Robino A,Rose LM,Rudolph A,Salomaa V,Sanna S,Schlessinger D,Schmidt MK,Southey MC,Sovio U,Stampfer MJ,St?ckl D,Storniolo AM,Timpson NJ,Tyrer J,Visser JA,Vollenweider P,V?lzke H,Waeber G,Waldenberger M,Wallaschofski H,Wang Q,Willemsen G,Winqvist R,Wolffenbuttel BH,Wright MJ,,,,,,,Boomsma DI,Econs MJ,Khaw KT,Loos RJ,McCarthy MI,Montgomery GW,Rice JP,Streeten EA,Thorsteinsdottir U,van Duijn CM,Alizadeh BZ,Bergmann S,Boerwinkle E,Boyd HA,Crisponi L,Gasparini P,Gieger C,Harris TB,Ingelsson E,J?rvelin MR,Kraft P,Lawlor D,Metspalu A,Pennell CE,Ridker PM,Snieder H,S?rensen TI,Spector TD,Strachan DP,Uitterlinden AG,Wareham NJ,Widen E,Zygmunt M,Murray A,Easton DF,Stefansson K,Murabito JM,Ong KK

Meta-analysis of genome-wide association studies in East Asian-ancestry populations identifies four new loci for body mass index.
Human molecular genetics23(20)5492-504(2014 Oct)
Wen W,Zheng W,Okada Y,Takeuchi F,Tabara Y,Hwang JY,Dorajoo R,Li H,Tsai FJ,Yang X,He J,Wu Y,He M,Zhang Y,Liang J,Guo X,Sheu WH,Delahanty R,Guo X,Kubo M,Yamamoto K,Ohkubo T,Go MJ,Liu JJ,Gan W,Chen CC,Gao Y,Li S,Lee NR,Wu C,Zhou X,Song H,Yao J,Lee IT,Long J,Tsunoda T,Akiyama K,Takashima N,Cho YS,Ong RT,Lu L,Chen CH,Tan A,Rice TK,Adair LS,Gui L,Allison M,Lee WJ,Cai Q,Isomura M,Umemura S,Kim YJ,Seielstad M,Hixson J,Xiang YB,Isono M,Kim BJ,Sim X,Lu W,Nabika T,Lee J,Lim WY,Gao YT,Takayanagi R,Kang DH,Wong TY,Hsiung CA,Wu IC,Juang JM,Shi J,Choi BY,Aung T,Hu F,Kim MK,Lim WY,Wang TD,Shin MH,Lee J,Ji BT,Lee YH,Young TL,Shin DH,Chun BY,Cho MC,Han BG,Hwu CM,Assimes TL,Absher D,Yan X,Kim E,Kuo JZ,Kwon S,Taylor KD,Chen YD,Rotter JI,Qi L,Zhu D,Wu T,Mohlke KL,Gu D,Mo Z,Wu JY,Lin X,Miki T,Tai ES,Lee JY,Kato N,Shu XO,Tanaka T

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SEC16B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

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