MBD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MBD2
Entrez Gene ID	8932
Full Name	methyl-CpG binding domain protein 2
Synonyms	DMTase,NY-CO-41
General protein information	Preferred Names methyl-CpG binding domain protein 2 Names methyl-CpG-binding domain protein 2 demethylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q21.2
Summary	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011].
Disorder MIM:	603547

mRNA and Protein(s)

mRNA	Protein	Name
NM_003927.5	NP_003918.1	methyl-CpG-binding domain protein 2 isoform 1
NM_015832.4	NP_056647.1	methyl-CpG-binding domain protein 2 testis-specific isoform
NM_015832.5	NP_056647.1	methyl-CpG-binding domain protein 2 testis-specific isoform
NM_003927.4	NP_003918.1	methyl-CpG-binding domain protein 2 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MBD2	XP_512139.4
Danio rerio (zebrafish)	mbd2	NP_997933.1
Homo sapiens (human)	MBD2	NP_003918.1
Bos taurus (cattle)	MBD2	XP_002697814.1
Rattus norvegicus (Norway rat)	Mbd2	NP_001108497.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003982	XP_003436675.1
	MBD2	XP_001082249.1
Mus musculus (house mouse)	Mbd2	NP_034903.2
Gallus gallus (chicken)	MBD2	NP_001012403.1
Canis lupus familiaris (dog)	MBD2	XP_005615735.1
Drosophila melanogaster (fruit fly)	MBD-like	NP_731370.1

Gene Ontology
Bibliography

Related articles in PubMed

MBD2 ablation impairs lymphopoiesis and impedes progression and maintenance of T-ALL.
Zhou M, Zhou K, Cheng L, Chen X, Wang J, Wang XM, Zhang Y, Yu Q, Zhang S, Wang D, Huang L, Huang M, Ma D, Cheng T, Wang CY, Yuan W, Zhou J
Cancer research(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CpG and methylation-dependent DNA binding and dynamics of the MBD2 protein at the single-molecule level have been reported.
Title: CpG and methylation-dependent DNA binding and dynamics of the methylcytosine binding domain 2 protein at the single-molecule level.

MBD2 has a critical role in 'rewriting' the cancer methylome at specific regulatory regions.
Title: Methyl-CpG-binding protein MBD2 plays a key role in maintenance and spread of DNA methylation at CpG islands and shores in cancer.

MBD2 expression was elevated in HCC tissue, which suggesting MBD2 as a candidate prognostic marker of HCC.
Title: MBD2 as a novel marker associated with poor survival of patients with hepatocellular carcinoma after hepatic resection.

Results show that MBD2 mediates epigenetic silencing of Htra1.
Title: Epigenetic silencing of serine protease HTRA1 drives polyploidy.

Specifically, methyl-CpG-binding domain protein 2 (MBD2) is revealed to be recruited to DNA damage sites after laser microirradiation, which was mediated through MBD domain and MBD2 C-terminus.
Title: iTRAQ-based chromatin proteomic screen reveals CHD4-dependent recruitment of MBD2 to sites of DNA damage.

The following MBD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21749	NM_003927.5 Latest version!	Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu26629	NM_015832.5 Latest version!	Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu26629	NM_015832.4	Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu21749	NM_003927.4	Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MBD2 ( NM_003927.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003918.1 Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21749
Clone ID Related Accession (Same CDS sequence)	NM_003927.5 , NM_003927.4
Accession Version	NM_003927.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF072242.1, AC090666.6 and AW151547.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003927.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072242.1, SRR1163655.427425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000256429.8/ ENSP00000256429.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGCGCGC ACCCGGGGGG AGGCCGCTGC TGCCCGGAGC AGGAGGAGGG GGAGAGTGCG 
GCGGGCGGCA GCGGCGCTGG CGGCGACTCC GCCATAGAGC AGGGGGGCCA GGGCAGCGCG 
CTCGCCCCGT CCCCGGTGAG CGGCGTGCGC AGGGAAGGCG CTCGGGGCGG CGGCCGTGGC 
CGGGGGCGGT GGAAGCAGGC GGGCCGGGGC GGCGGCGTCT GTGGCCGTGG CCGGGGCCGG 
GGCCGTGGCC GGGGACGGGG ACGGGGCCGG GGCCGGGGCC GCGGCCGTCC CCCGAGTGGC 
GGCAGCGGCC TTGGCGGCGA CGGCGGCGGC TGCGGCGGCG GCGGCAGCGG TGGCGGCGGC 
GCCCCCCGGC GGGAGCCGGT CCCTTTCCCG TCGGGGAGCG CGGGGCCGGG GCCCAGGGGA 
CCCCGGGCCA CGGAGAGCGG GAAGAGGATG GATTGCCCGG CCCTCCCCCC CGGATGGAAG 
AAGGAGGAAG TGATCCGAAA ATCTGGGCTA AGTGCTGGCA AGAGCGATGT CTACTACTTC 
AGTCCAAGTG GTAAGAAGTT CAGAAGCAAG CCTCAGTTGG CAAGGTACCT GGGAAATACT 
GTTGATCTCA GCAGTTTTGA CTTCAGAACT GGAAAGATGA TGCCTAGTAA ATTACAGAAG 
AACAAACAGA GACTGCGAAA CGATCCTCTC AATCAAAATA AGGGTAAACC AGACTTGAAT 
ACAACATTGC CAATTAGACA AACAGCATCA ATTTTCAAAC AACCGGTAAC CAAAGTCACA 
AATCATCCTA GTAATAAAGT GAAATCAGAC CCACAACGAA TGAATGAACA GCCACGTCAG 
CTTTTCTGGG AGAAGAGGCT ACAAGGACTT AGTGCATCAG ATGTAACAGA ACAAATTATA 
AAAACCATGG AACTACCCAA AGGTCTTCAA GGAGTTGGTC CAGGTAGCAA TGATGAGACC 
CTTTTATCTG CTGTTGCCAG TGCTTTGCAC ACAAGCTCTG CGCCAATCAC AGGGCAAGTC 
TCCGCTGCTG TGGAAAAGAA CCCTGCTGTT TGGCTTAACA CATCTCAACC CCTCTGCAAA 
GCTTTTATTG TCACAGATGA AGACATCAGG AAACAGGAAG AGCGAGTACA GCAAGTACGC 
AAGAAATTGG AAGAAGCACT GATGGCAGAC ATCTTGTCGC GAGCTGCTGA TACAGAAGAG 
ATGGATATTG AAATGGACAG TGGAGATGAA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003918.1
CDS	111..1346
Translation	MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAGRGGGVCGRGRGRGRGRGRGRGRGRGRGRPPSGGSGLGGDGGGCGGGGSGGGGAPRREPVPFPSGSAGPGPRGPRATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNTVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQNKGKPDLNTTLPIRQTASIFKQPVTKVTNHPSNKVKSDPQRMNEQPRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSSAPITGQVSAAVEKNPAVWLNTSQPLCKAFIVTDEDIRKQEERVQQVRKKLEEALMADILSRAADTEEMDIEMDSGDEA

Target ORF information:

RefSeq Version	NM_003927.5
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003927.5

ORF Insert Sequence:

ATGCGCGCGC ACCCGGGGGG AGGCCGCTGC TGCCCGGAGC AGGAGGAGGG GGAGAGTGCG 
GCGGGCGGCA GCGGCGCTGG CGGCGACTCC GCCATAGAGC AGGGGGGCCA GGGCAGCGCG 
CTCGCCCCGT CCCCGGTGAG CGGCGTGCGC AGGGAAGGCG CTCGGGGCGG CGGCCGTGGC 
CGGGGGCGGT GGAAGCAGGC GGGCCGGGGC GGCGGCGTCT GTGGCCGTGG CCGGGGCCGG 
GGCCGTGGCC GGGGACGGGG ACGGGGCCGG GGCCGGGGCC GCGGCCGTCC CCCGAGTGGC 
GGCAGCGGCC TTGGCGGCGA CGGCGGCGGC TGCGGCGGCG GCGGCAGCGG TGGCGGCGGC 
GCCCCCCGGC GGGAGCCGGT CCCTTTCCCG TCGGGGAGCG CGGGGCCGGG GCCCAGGGGA 
CCCCGGGCCA CGGAGAGCGG GAAGAGGATG GATTGCCCGG CCCTCCCCCC CGGATGGAAG 
AAGGAGGAAG TGATCCGAAA ATCTGGGCTA AGTGCTGGCA AGAGCGATGT CTACTACTTC 
AGTCCAAGTG GTAAGAAGTT CAGAAGCAAG CCTCAGTTGG CAAGGTACCT GGGAAATACT 
GTTGATCTCA GCAGTTTTGA CTTCAGAACT GGAAAGATGA TGCCTAGTAA ATTACAGAAG 
AACAAACAGA GACTGCGAAA CGATCCTCTC AATCAAAATA AGGGTAAACC AGACTTGAAT 
ACAACATTGC CAATTAGACA AACAGCATCA ATTTTCAAAC AACCGGTAAC CAAAGTCACA 
AATCATCCTA GTAATAAAGT GAAATCAGAC CCACAACGAA TGAATGAACA GCCACGTCAG 
CTTTTCTGGG AGAAGAGGCT ACAAGGACTT AGTGCATCAG ATGTAACAGA ACAAATTATA 
AAAACCATGG AACTACCCAA AGGTCTTCAA GGAGTTGGTC CAGGTAGCAA TGATGAGACC 
CTTTTATCTG CTGTTGCCAG TGCTTTGCAC ACAAGCTCTG CGCCAATCAC AGGGCAAGTC 
TCCGCTGCTG TGGAAAAGAA CCCTGCTGTT TGGCTTAACA CATCTCAACC CCTCTGCAAA 
GCTTTTATTG TCACAGATGA AGACATCAGG AAACAGGAAG AGCGAGTACA GCAAGTACGC 
AAGAAATTGG AAGAAGCACT GATGGCAGAC ATCTTGTCGC GAGCTGCTGA TACAGAAGAG 
ATGGATATTG AAATGGACAG TGGAGATGAA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD2 ( NM_003927.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003918.1 Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21749
Clone ID Related Accession (Same CDS sequence)	NM_003927.5 , NM_003927.4
Accession Version	NM_003927.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-15
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF072242.1, AC090666.6 and AW151547.1. This sequence is a reference standard in the RefSeqGene project. On Feb 9, 2011 this sequence version replaced NM_003927.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072242.1, SRR1163655.427425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGCGCGC ACCCGGGGGG AGGCCGCTGC TGCCCGGAGC AGGAGGAGGG GGAGAGTGCG 
GCGGGCGGCA GCGGCGCTGG CGGCGACTCC GCCATAGAGC AGGGGGGCCA GGGCAGCGCG 
CTCGCCCCGT CCCCGGTGAG CGGCGTGCGC AGGGAAGGCG CTCGGGGCGG CGGCCGTGGC 
CGGGGGCGGT GGAAGCAGGC GGGCCGGGGC GGCGGCGTCT GTGGCCGTGG CCGGGGCCGG 
GGCCGTGGCC GGGGACGGGG ACGGGGCCGG GGCCGGGGCC GCGGCCGTCC CCCGAGTGGC 
GGCAGCGGCC TTGGCGGCGA CGGCGGCGGC TGCGGCGGCG GCGGCAGCGG TGGCGGCGGC 
GCCCCCCGGC GGGAGCCGGT CCCTTTCCCG TCGGGGAGCG CGGGGCCGGG GCCCAGGGGA 
CCCCGGGCCA CGGAGAGCGG GAAGAGGATG GATTGCCCGG CCCTCCCCCC CGGATGGAAG 
AAGGAGGAAG TGATCCGAAA ATCTGGGCTA AGTGCTGGCA AGAGCGATGT CTACTACTTC 
AGTCCAAGTG GTAAGAAGTT CAGAAGCAAG CCTCAGTTGG CAAGGTACCT GGGAAATACT 
GTTGATCTCA GCAGTTTTGA CTTCAGAACT GGAAAGATGA TGCCTAGTAA ATTACAGAAG 
AACAAACAGA GACTGCGAAA CGATCCTCTC AATCAAAATA AGGGTAAACC AGACTTGAAT 
ACAACATTGC CAATTAGACA AACAGCATCA ATTTTCAAAC AACCGGTAAC CAAAGTCACA 
AATCATCCTA GTAATAAAGT GAAATCAGAC CCACAACGAA TGAATGAACA GCCACGTCAG 
CTTTTCTGGG AGAAGAGGCT ACAAGGACTT AGTGCATCAG ATGTAACAGA ACAAATTATA 
AAAACCATGG AACTACCCAA AGGTCTTCAA GGAGTTGGTC CAGGTAGCAA TGATGAGACC 
CTTTTATCTG CTGTTGCCAG TGCTTTGCAC ACAAGCTCTG CGCCAATCAC AGGGCAAGTC 
TCCGCTGCTG TGGAAAAGAA CCCTGCTGTT TGGCTTAACA CATCTCAACC CCTCTGCAAA 
GCTTTTATTG TCACAGATGA AGACATCAGG AAACAGGAAG AGCGAGTACA GCAAGTACGC 
AAGAAATTGG AAGAAGCACT GATGGCAGAC ATCTTGTCGC GAGCTGCTGA TACAGAAGAG 
ATGGATATTG AAATGGACAG TGGAGATGAA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003918.1
CDS	230..1465
Translation	MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAGRGGGVCGRGRGRGRGRGRGRGRGRGRGRPPSGGSGLGGDGGGCGGGGSGGGGAPRREPVPFPSGSAGPGPRGPRATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNTVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQNKGKPDLNTTLPIRQTASIFKQPVTKVTNHPSNKVKSDPQRMNEQPRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSSAPITGQVSAAVEKNPAVWLNTSQPLCKAFIVTDEDIRKQEERVQQVRKKLEEALMADILSRAADTEEMDIEMDSGDEA

Target ORF information:

RefSeq Version	NM_003927.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003927.4

ORF Insert Sequence:

ATGCGCGCGC ACCCGGGGGG AGGCCGCTGC TGCCCGGAGC AGGAGGAGGG GGAGAGTGCG 
GCGGGCGGCA GCGGCGCTGG CGGCGACTCC GCCATAGAGC AGGGGGGCCA GGGCAGCGCG 
CTCGCCCCGT CCCCGGTGAG CGGCGTGCGC AGGGAAGGCG CTCGGGGCGG CGGCCGTGGC 
CGGGGGCGGT GGAAGCAGGC GGGCCGGGGC GGCGGCGTCT GTGGCCGTGG CCGGGGCCGG 
GGCCGTGGCC GGGGACGGGG ACGGGGCCGG GGCCGGGGCC GCGGCCGTCC CCCGAGTGGC 
GGCAGCGGCC TTGGCGGCGA CGGCGGCGGC TGCGGCGGCG GCGGCAGCGG TGGCGGCGGC 
GCCCCCCGGC GGGAGCCGGT CCCTTTCCCG TCGGGGAGCG CGGGGCCGGG GCCCAGGGGA 
CCCCGGGCCA CGGAGAGCGG GAAGAGGATG GATTGCCCGG CCCTCCCCCC CGGATGGAAG 
AAGGAGGAAG TGATCCGAAA ATCTGGGCTA AGTGCTGGCA AGAGCGATGT CTACTACTTC 
AGTCCAAGTG GTAAGAAGTT CAGAAGCAAG CCTCAGTTGG CAAGGTACCT GGGAAATACT 
GTTGATCTCA GCAGTTTTGA CTTCAGAACT GGAAAGATGA TGCCTAGTAA ATTACAGAAG 
AACAAACAGA GACTGCGAAA CGATCCTCTC AATCAAAATA AGGGTAAACC AGACTTGAAT 
ACAACATTGC CAATTAGACA AACAGCATCA ATTTTCAAAC AACCGGTAAC CAAAGTCACA 
AATCATCCTA GTAATAAAGT GAAATCAGAC CCACAACGAA TGAATGAACA GCCACGTCAG 
CTTTTCTGGG AGAAGAGGCT ACAAGGACTT AGTGCATCAG ATGTAACAGA ACAAATTATA 
AAAACCATGG AACTACCCAA AGGTCTTCAA GGAGTTGGTC CAGGTAGCAA TGATGAGACC 
CTTTTATCTG CTGTTGCCAG TGCTTTGCAC ACAAGCTCTG CGCCAATCAC AGGGCAAGTC 
TCCGCTGCTG TGGAAAAGAA CCCTGCTGTT TGGCTTAACA CATCTCAACC CCTCTGCAAA 
GCTTTTATTG TCACAGATGA AGACATCAGG AAACAGGAAG AGCGAGTACA GCAAGTACGC 
AAGAAATTGG AAGAAGCACT GATGGCAGAC ATCTTGTCGC GAGCTGCTGA TACAGAAGAG 
ATGGATATTG AAATGGACAG TGGAGATGAA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD2 ( NM_015832.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056647.1 Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26629
Clone ID Related Accession (Same CDS sequence)	NM_015832.4 , NM_015832.5
Accession Version	NM_015832.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-22
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 2 testis-specific isoform
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF072242.1, AF072246.1 and AW274866.1. On Feb 9, 2011 this sequence version replaced NM_015832.3. Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region, compared to variant 1, resulting in a shorter protein (testis-specific) that has a shorter C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMN03267758 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGCGCGC ACCCGGGGGG AGGCCGCTGC TGCCCGGAGC AGGAGGAGGG GGAGAGTGCG 
GCGGGCGGCA GCGGCGCTGG CGGCGACTCC GCCATAGAGC AGGGGGGCCA GGGCAGCGCG 
CTCGCCCCGT CCCCGGTGAG CGGCGTGCGC AGGGAAGGCG CTCGGGGCGG CGGCCGTGGC 
CGGGGGCGGT GGAAGCAGGC GGGCCGGGGC GGCGGCGTCT GTGGCCGTGG CCGGGGCCGG 
GGCCGTGGCC GGGGACGGGG ACGGGGCCGG GGCCGGGGCC GCGGCCGTCC CCCGAGTGGC 
GGCAGCGGCC TTGGCGGCGA CGGCGGCGGC TGCGGCGGCG GCGGCAGCGG TGGCGGCGGC 
GCCCCCCGGC GGGAGCCGGT CCCTTTCCCG TCGGGGAGCG CGGGGCCGGG GCCCAGGGGA 
CCCCGGGCCA CGGAGAGCGG GAAGAGGATG GATTGCCCGG CCCTCCCCCC CGGATGGAAG 
AAGGAGGAAG TGATCCGAAA ATCTGGGCTA AGTGCTGGCA AGAGCGATGT CTACTACTTC 
AGTCCAAGTG GTAAGAAGTT CAGAAGCAAG CCTCAGTTGG CAAGGTACCT GGGAAATACT 
GTTGATCTCA GCAGTTTTGA CTTCAGAACT GGAAAGATGA TGCCTAGTAA ATTACAGAAG 
AACAAACAGA GACTGCGAAA CGATCCTCTC AATCAAAATA AGCTGCGCTG GAACACTCAT 
CGTCCTGCAC CATGGCATGC GCTTTCAAGA CTCTGCTTGC TCATACGCTG TTTGCTCTGC 
TTGGAATGTG CTTACCCCCT TCCCCTTCAT CTGGTGAACT CCTACTCATC CAAGACCCAG 
CTTCATTGTC TCCATCTCTG GGAAGCCTGC CCTGCATACT CCAGGCAGAA CCAATCCTTT 
CCTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056647.1
CDS	230..1138
Translation	MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAGRGGGVCGRGRGRGRGRGRGRGRGRGRGRPPSGGSGLGGDGGGCGGGGSGGGGAPRREPVPFPSGSAGPGPRGPRATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNTVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQNKLRWNTHRPAPWHALSRLCLLIRCLLCLECAYPLPLHLVNSYSSKTQLHCLHLWEACPAYSRQNQSFPP

Target ORF information:

RefSeq Version	NM_015832.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015832.4

ORF Insert Sequence:

ATGCGCGCGC ACCCGGGGGG AGGCCGCTGC TGCCCGGAGC AGGAGGAGGG GGAGAGTGCG 
GCGGGCGGCA GCGGCGCTGG CGGCGACTCC GCCATAGAGC AGGGGGGCCA GGGCAGCGCG 
CTCGCCCCGT CCCCGGTGAG CGGCGTGCGC AGGGAAGGCG CTCGGGGCGG CGGCCGTGGC 
CGGGGGCGGT GGAAGCAGGC GGGCCGGGGC GGCGGCGTCT GTGGCCGTGG CCGGGGCCGG 
GGCCGTGGCC GGGGACGGGG ACGGGGCCGG GGCCGGGGCC GCGGCCGTCC CCCGAGTGGC 
GGCAGCGGCC TTGGCGGCGA CGGCGGCGGC TGCGGCGGCG GCGGCAGCGG TGGCGGCGGC 
GCCCCCCGGC GGGAGCCGGT CCCTTTCCCG TCGGGGAGCG CGGGGCCGGG GCCCAGGGGA 
CCCCGGGCCA CGGAGAGCGG GAAGAGGATG GATTGCCCGG CCCTCCCCCC CGGATGGAAG 
AAGGAGGAAG TGATCCGAAA ATCTGGGCTA AGTGCTGGCA AGAGCGATGT CTACTACTTC 
AGTCCAAGTG GTAAGAAGTT CAGAAGCAAG CCTCAGTTGG CAAGGTACCT GGGAAATACT 
GTTGATCTCA GCAGTTTTGA CTTCAGAACT GGAAAGATGA TGCCTAGTAA ATTACAGAAG 
AACAAACAGA GACTGCGAAA CGATCCTCTC AATCAAAATA AGCTGCGCTG GAACACTCAT 
CGTCCTGCAC CATGGCATGC GCTTTCAAGA CTCTGCTTGC TCATACGCTG TTTGCTCTGC 
TTGGAATGTG CTTACCCCCT TCCCCTTCAT CTGGTGAACT CCTACTCATC CAAGACCCAG 
CTTCATTGTC TCCATCTCTG GGAAGCCTGC CCTGCATACT CCAGGCAGAA CCAATCCTTT 
CCTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD2 ( NM_015832.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056647.1 Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 2, mRNA.

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CloneID	OHu26629
Clone ID Related Accession (Same CDS sequence)	NM_015832.4 , NM_015832.5
Accession Version	NM_015832.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 2 testis-specific isoform
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF072242.1, AF072246.1 and AW274866.1. On Jun 1, 2019 this sequence version replaced NM_015832.4. Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region, compared to variant 1, resulting in a shorter protein (testis-specific) that has a shorter C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMN03267758 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGCGCGC ACCCGGGGGG AGGCCGCTGC TGCCCGGAGC AGGAGGAGGG GGAGAGTGCG 
GCGGGCGGCA GCGGCGCTGG CGGCGACTCC GCCATAGAGC AGGGGGGCCA GGGCAGCGCG 
CTCGCCCCGT CCCCGGTGAG CGGCGTGCGC AGGGAAGGCG CTCGGGGCGG CGGCCGTGGC 
CGGGGGCGGT GGAAGCAGGC GGGCCGGGGC GGCGGCGTCT GTGGCCGTGG CCGGGGCCGG 
GGCCGTGGCC GGGGACGGGG ACGGGGCCGG GGCCGGGGCC GCGGCCGTCC CCCGAGTGGC 
GGCAGCGGCC TTGGCGGCGA CGGCGGCGGC TGCGGCGGCG GCGGCAGCGG TGGCGGCGGC 
GCCCCCCGGC GGGAGCCGGT CCCTTTCCCG TCGGGGAGCG CGGGGCCGGG GCCCAGGGGA 
CCCCGGGCCA CGGAGAGCGG GAAGAGGATG GATTGCCCGG CCCTCCCCCC CGGATGGAAG 
AAGGAGGAAG TGATCCGAAA ATCTGGGCTA AGTGCTGGCA AGAGCGATGT CTACTACTTC 
AGTCCAAGTG GTAAGAAGTT CAGAAGCAAG CCTCAGTTGG CAAGGTACCT GGGAAATACT 
GTTGATCTCA GCAGTTTTGA CTTCAGAACT GGAAAGATGA TGCCTAGTAA ATTACAGAAG 
AACAAACAGA GACTGCGAAA CGATCCTCTC AATCAAAATA AGCTGCGCTG GAACACTCAT 
CGTCCTGCAC CATGGCATGC GCTTTCAAGA CTCTGCTTGC TCATACGCTG TTTGCTCTGC 
TTGGAATGTG CTTACCCCCT TCCCCTTCAT CTGGTGAACT CCTACTCATC CAAGACCCAG 
CTTCATTGTC TCCATCTCTG GGAAGCCTGC CCTGCATACT CCAGGCAGAA CCAATCCTTT 
CCTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056647.1
CDS	111..1019
Translation	MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAGRGGGVCGRGRGRGRGRGRGRGRGRGRGRPPSGGSGLGGDGGGCGGGGSGGGGAPRREPVPFPSGSAGPGPRGPRATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNTVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQNKLRWNTHRPAPWHALSRLCLLIRCLLCLECAYPLPLHLVNSYSSKTQLHCLHLWEACPAYSRQNQSFPP

Target ORF information:

RefSeq Version	NM_015832.5
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015832.5

ORF Insert Sequence:

ATGCGCGCGC ACCCGGGGGG AGGCCGCTGC TGCCCGGAGC AGGAGGAGGG GGAGAGTGCG 
GCGGGCGGCA GCGGCGCTGG CGGCGACTCC GCCATAGAGC AGGGGGGCCA GGGCAGCGCG 
CTCGCCCCGT CCCCGGTGAG CGGCGTGCGC AGGGAAGGCG CTCGGGGCGG CGGCCGTGGC 
CGGGGGCGGT GGAAGCAGGC GGGCCGGGGC GGCGGCGTCT GTGGCCGTGG CCGGGGCCGG 
GGCCGTGGCC GGGGACGGGG ACGGGGCCGG GGCCGGGGCC GCGGCCGTCC CCCGAGTGGC 
GGCAGCGGCC TTGGCGGCGA CGGCGGCGGC TGCGGCGGCG GCGGCAGCGG TGGCGGCGGC 
GCCCCCCGGC GGGAGCCGGT CCCTTTCCCG TCGGGGAGCG CGGGGCCGGG GCCCAGGGGA 
CCCCGGGCCA CGGAGAGCGG GAAGAGGATG GATTGCCCGG CCCTCCCCCC CGGATGGAAG 
AAGGAGGAAG TGATCCGAAA ATCTGGGCTA AGTGCTGGCA AGAGCGATGT CTACTACTTC 
AGTCCAAGTG GTAAGAAGTT CAGAAGCAAG CCTCAGTTGG CAAGGTACCT GGGAAATACT 
GTTGATCTCA GCAGTTTTGA CTTCAGAACT GGAAAGATGA TGCCTAGTAA ATTACAGAAG 
AACAAACAGA GACTGCGAAA CGATCCTCTC AATCAAAATA AGCTGCGCTG GAACACTCAT 
CGTCCTGCAC CATGGCATGC GCTTTCAAGA CTCTGCTTGC TCATACGCTG TTTGCTCTGC 
TTGGAATGTG CTTACCCCCT TCCCCTTCAT CTGGTGAACT CCTACTCATC CAAGACCCAG 
CTTCATTGTC TCCATCTCTG GGAAGCCTGC CCTGCATACT CCAGGCAGAA CCAATCCTTT 
CCTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MBD2 ablation impairs lymphopoiesis and impedes progression and maintenance of T-ALL.
Cancer research(2018 Jan)
Zhou M,Zhou K,Cheng L,Chen X,Wang J,Wang XM,Zhang Y,Yu Q,Zhang S,Wang D,Huang L,Huang M,Ma D,Cheng T,Wang CY,Yuan W,Zhou J

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MBD2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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