BSN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BSN
Entrez Gene ID	8927
Full Name	bassoon presynaptic cytomatrix protein
Synonyms	ZNF231
General protein information	Preferred Names bassoon presynaptic cytomatrix protein Names protein bassoon neuronal double zinc finger protein zinc finger protein 231
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Summary	Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses. [provided by RefSeq, Jul 2008].
Disorder MIM:	604020

mRNA and Protein(s)

mRNA	Protein	Name
NM_003458.3	NP_003449.2	protein bassoon
NM_003458.4	NP_003449.2	protein bassoon

Pathways from BioSystems

Homologies

	BSN	XP_001094100.2
Canis lupus familiaris (dog)	BSN	XP_541885.2
Mus musculus (house mouse)	Bsn	NP_031593.2
Homo sapiens (human)	BSN	NP_003449.2
Rattus norvegicus (Norway rat)	Bsn	NP_062019.2
Gallus gallus (chicken)	BSN	XP_004944501.1
Pan troglodytes (chimpanzee)	BSN	XP_516463.4
Bos taurus (cattle)	BSN	XP_002697122.2
Xenopus tropicalis (tropical clawed frog)	bsn	XP_002936530.2
Danio rerio (zebrafish)	LOC100004063	XP_001920788.3

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in bassoon in individuals with familial and sporadic progressive supranuclear palsy-like syndrome.
Yabe I, Yaguchi H, Kato Y, Miki Y, Takahashi H, Tanikawa S, Shirai S, Takahashi I, Kimura M, Hama Y, Matsushima M, Fujioka S, Kano T, Watanabe M, Nakagawa S, Kunieda Y, Ikeda Y, Hasegawa M, Nishihara H, Ohtsuka T, Tanaka S, Tsuboi Y, Hatakeyama S, Wakabayashi K, Sasaki H
Scientific reports8(1)819(2018 Jan)

A genome-wide association study identifies a novel locus at 6q22.1 associated with ulcerative colitis.
Juli? A, Dom?nech E, Chaparro M, Garc?a-S?nchez V, Gomoll?n F, Pan?s J, Ma?osa M, Barreiro-De Acosta M, Guti?rrez A, Garcia-Planella E, Aguas M, Mu?oz F, Esteve M, Mendoza JL, Vera M, M?rquez L, Tortosa R, L?pez-Lasanta M, Alonso A, Gelp? JL, Garc?a-Montero AC, Bertranpetit J, Absher D, Myers RM, Gisbert JP, Marsal S
Human molecular genetics23(25)6927-34(2014 Dec)

Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
Jostins L, Ripke S, Weersma RK, Duerr RH, McGovern DP, Hui KY, Lee JC, Schumm LP, Sharma Y, Anderson CA, Essers J, Mitrovic M, Ning K, Cleynen I, Theatre E, Spain SL, Raychaudhuri S, Goyette P, Wei Z, Abraham C, Achkar JP, Ahmad T, Amininejad L, Ananthakrishnan AN, Andersen V, Andrews JM, Baidoo L, Balschun T, Bampton PA, Bitton A, Boucher G, Brand S, B?ning C, Cohain A, Cichon S, D'Amato M, De Jong D, Devaney KL, Dubinsky M, Edwards C, Ellinghaus D, Ferguson LR, Franchimont D, Fransen K, Gearry R, Georges M, Gieger C, Glas J, Haritunians T, Hart A, Hawkey C, Hedl M, Hu X, Karlsen TH, Kupcinskas L, Kugathasan S, Latiano A, Laukens D, Lawrance IC, Lees CW, Louis E, Mahy G, Mansfield J, Morgan AR, Mowat C, Newman W, Palmieri O, Ponsioen CY, Potocnik U, Prescott NJ, Regueiro M, Rotter JI, Russell RK, Sanderson JD, Sans M, Satsangi J, Schreiber S, Simms LA, Sventoraityte J, Targan SR, Taylor KD, Tremelling M, Verspaget HW, De Vos M, Wijmenga C, Wilson DC, Winkelmann J, Xavier RJ, Zeissig S, Zhang B, Zhang CK, Zhao H, , Silverberg MS, Annese V, Hakonarson H, Brant SR, Radford-Smith G, Mathew CG, Rioux JD, Schadt EE, Daly MJ, Franke A, Parkes M, Vermeire S, Barrett JC, Cho JH
Nature491(7422)119-24(2012 Nov)

D-amino acid oxidase activity is inhibited by an interaction with bassoon protein at the presynaptic active zone.
Popiolek M, Ross JF, Charych E, Chanda P, Gundelfinger ED, Moss SJ, Brandon NJ, Pausch MH
The Journal of biological chemistry286(33)28867-75(2011 Aug)

Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.
Martins-de-Souza D, Gattaz WF, Schmitt A, Rewerts C, Maccarrone G, Dias-Neto E, Turck CW
European archives of psychiatry and clinical neuroscience259(3)151-63(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

D-amino acid oxidase activity is inhibited by an interaction with bassoon protein at the presynaptic active zone.
Title: D-amino acid oxidase activity is inhibited by an interaction with bassoon protein at the presynaptic active zone.

Bassoon protein and the synaptic ribbon create a large number of release sites by organizing calcium channels and synaptic vesicles.
Title: Bassoon and the synaptic ribbon organize Ca?+ channels and vesicles to add release sites and promote refilling.

Short-term synaptic depression is enhanced in Bassoon knockout mice, but synaptic transmission is unaffected.
Title: Bassoon speeds vesicle reloading at a central excitatory synapse.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association of linear growth impairment in pediatric Crohn's disease and a known height locus: a pilot study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association analysis of 3p21 with Crohn's disease in a New Zealand population.

The following BSN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BSN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19210	NM_003458.4 Latest version!	Homo sapiens bassoon presynaptic cytomatrix protein (BSN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu19210	NM_003458.3	Homo sapiens bassoon presynaptic cytomatrix protein (BSN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BSN ( NM_003458.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003449.2 Homo sapiens bassoon presynaptic cytomatrix protein (BSN), mRNA.

Price & Availability↑

CloneID	OHu19210
Clone ID Related Accession (Same CDS sequence)	NM_003458.3 , NM_003458.4
Accession Version	NM_003458.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	protein bassoon
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF052224.1, AC099668.2, AB007894.1 and AW131629.1. This sequence is a reference standard in the RefSeqGene project. On Jul 4, 2007 this sequence version replaced NM_003458.2. CCDS Note: This CCDS ID represents the protein described in PMIDs: 10329005 and 9679147. This transcript is supported by AF052224.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMIDs: 9679147 and 19165527. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF052224.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 10329005 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACG AGGTCAGCCT GGAGGGCGGC GCTGGCGACG GGCCGCTGCC GCCCGGCGGC 
GCCGGCCCCG GCCCGGGCCC CGGCCCCGGC CCCGGCGCAG GAAAGCCGCC TTCAGCACCG 
GCCGGTGGCG GACAGCTCCC CGCGGCGGGA GCAGCGCGGT CGACCGCGGT ACCACCGGTC 
CCTGGCCCCG GCCCCGGCCC CGGTCCCGGC CCTGGCCCGG GCAGCACTTC CCGGAGACTG 
GACCCCAAGG AACCCCTGGG TAACCAGAGA GCAGCTTCCC CAACTCCGAA GCAGGCTTCT 
GCTACCACTC CTGGCCATGA GAGCCCCCGA GAGACAAGGG CACAGGGACC AGCAGGCCAG 
GAGGCTGATG GTCCCCGCAG GACGCTGCAG GTAGACAGCA GGACACAGAG ATCAGGGCGG 
TCCCCCTCAG TGTCACCGGA CAGAGGCAGC ACCCCCACAT CACCCTACTC CGTCCCTCAG 
ATCGCCCCCC TTCCCAGCAG CACGCTGTGT CCCATATGCA AGACTTCGGA CCTCACGTCG 
ACCCCCAGCC AGCCAAACTT CAACACCTGC ACCCAGTGTC ACAACAAGGT CTGCAACCAG 
TGTGGGTTCA ACCCCAACCC TCATCTCACC CAGGTGAAGG AGTGGCTCTG TCTGAACTGT 
CAGATGCAGA GGGCTCTGGG AATGGACATG ACCACTGCAC CTCGCTCCAA GAGCCAGCAG 
CAGCTGCACT CCCCAGCCCT GTCTCCTGCC CACTCCCCGG CCAAACAGCC CCTGGGGAAG 
CCAGACCAAG AGAGATCTCG GGGCCCAGGA GGACCACAGC CTGGGTCCCG CCAGGCTGAG 
ACAGCCAGGG CCACCTCAGT GCCGGGGCCT GCCCAAGCAG CTGCCCCTCC AGAGGTGGGG 
AGGGTGTCTC CTCAGCCCCC TCAACCCACC AAGCCTTCCA CAGCTGAGCC CAGGCCACCT 
GCAGGAGAGG CCCCGGCCAA AAGTGCCACC GCAGTGCCCG CTGGGCTTGG TGCCACTGAG 
CAGACCCAGG AGGGCCTCAC TGGTAAGCTC TTCGGCCTTG GCGCGTCACT GCTAACCCAG 
GCGAGCACCC TCATGTCTGT GCAGCCCGAG GCTGACACCC AGGGCCAGCC TGCCCCCAGC 
AAGGGGACAC CTAAGATCGT CTTCAATGAT GCCAGCAAGG AGGCTGGCCC AAAACCCTTG 
GGCTCAGGGC CCGGGCCTGG GCCAGCACCT GGAGCCAAAA CTGAGCCTGG GGCTAGAATG 
GGTCCTGGAT CTGGACCTGG AGCCCTGCCG AAAACTGGGG GAACAACCAG TCCAAAGCAT 
GGCAGAGCAG AACATCAGGC AGCATCGAAG GCTGCTGCCA AGCCAAAGAC CATGCCGAAG 
GAAAGGGCCA TCTGCCCACT GTGCCAAGCC GAGCTCAACG TGGGCAGCAA GAGCCCAGCC 
AACTATAACA CATGCACCAC CTGCAGGCTC CAGGTGTGCA ACCTGTGTGG CTTCAACCCA 
ACACCCCACC TGGTGGAGAA AACAGAGTGG CTCTGTCTGA ACTGCCAAAC CAAGCGGCTA 
CTGGAGGGCA GCCTAGGAGA GCCGACCCCC CTGCCGCCGC CCACCTCACA GCAGCCCCCT 
GTAGGGGCCC CTCACCGTGC ATCTGGAACA TCCCCTCTGA AGCAGAAAGG GCCACAGGGG 
CTGGGCCAGC CTTCAGGCCC CCTGCCTGCC AAGGCCAGCC CTCTATCCAC CAAGGCCAGC 
CCTCTGCCCA GCAAGGCCAG CCCCCAGGCC AAGCCCCTCA GGGCTTCTGA ACCCAGCAAG 
ACCCCAAGCA GTGTCCAGGA AAAGAAGACC CGAGTCCCCA CTAAAGCTGA GCCCATGCCG 
AAGCCACCTC CAGAGACTAC CCCAACCCCT GCGACTCCTA AAGTAAAGAG TGGGGTGAGG 
AGGGCTGAAC CTGCCACCCC TGTCGTCAAG GCTGTTCCAG AAGCCCCCAA GGGTGGGGAG 
GCGGAGGACC TGGTGGGCAA GCCTTACTCT CAGGATGCGT CTCGGAGCCC ACAGAGCCTC 
AGTGACACAG GCTATTCCTC TGACGGCATC TCTAGCTCCC AGAGTGAGAT CACAGGAGTC 
GTGCAGCAGG AGGTGGAACA GCTGGACAGT GCAGGGGTGA CAGGGCCACA TCCACCCAGC 
CCCTCCGAGA TCCACAAGGT GGGGAGCAGC ATGCGGCCTT TGCTGCAGGC CCAGGGCCTG 
GCCCCAAGTG AGCGGAGCAA GCCACTCTCC AGCGGTACTG GCGAGGAGCA GAAGCAGCGG 
CCCCACTCCT TGTCCATCAC GCCTGAGGCC TTTGACTCTG ATGAGGAGCT GGAGGATATC 
CTGGAGGAAG ACGAAGACTC TGCTGAGTGG AGGCGCCGGA GAGAGCAGCA GGACACTGCC 
GAGTCCTCAG ACGACTTTGG CAGCCAATTG AGGCACGACT ATGTGGAGGA CAGCAGTGAG 
GGTGGCCTGT CCCCTCTTCC ACCCCAGCCC CCAGCCCGGG CAGCAGAACT GACTGATGAG 
GATTTCATGC GACGGCAGAT TCTCGAGATG AGCGCCGAGG AAGACAACCT GGAGGAGGAT 
GACACTGCCA CCTCCGGGCG TGGCCTGGCC AAACATGGCA CCCAGAAAGG TGGCCCCAGA 
CCCAGGCCTG AGCCTAGCCA AGAACCAGCA GCACTGCCCA AGAGGCGCCT GCCCCACAAT 
GCCACCACGG GCTATGAGGA GCTGCTCCCT GAGGGAGGCT CAGCAGAGGC TACCGATGGC 
AGTGGGACCC TGCAGGGTGG GCTCCGTCGC TTCAAGACCA TTGAGCTCAA CAGCACGGGA 
AGTTATGGTC ATGAGTTGGA CCTGGGCCAA GGCCCAGACC CCAGTCTGGA CCGGGAGCCT 
GAGCTGGAGA TGGAGAGCCT AACGGGCTCC CCTGAGGACC GCTCCCGTGG TGAGCACTCC 
TCTACATTGC CTGCCTCCAC ACCCAGCTAC ACCTCGGGCA CCTCTCCCAC CTCTCTGTCC 
TCCCTAGAGG AGGACAGTGA CAGCAGCCCC AGCCGCAGGC AGCGTCTAGA AGAAGCAAAG 
CAGCAGCGCA AGGCCCGGCA CCGCTCCCAC GGGCCCCTGC TACCCACCAT CGAGGACTCC 
TCAGAGGAGG AGGAGCTGCG GGAGGAAGAG GAGCTGCTTC GTGAGCAAGA GAAGATGCGG 
GAGGTGGAGC AGCAGCGCAT CCGCAGCACG GCCCGCAAGA CCCGGCGGGA CAAGGAAGAA 
CTGCGGGCCC AGCGGAGGCG AGAGCGCTCC AAGACACCAC CCAGTAACTT GTCACCCATC 
GAGGATGCCT CCCCGACGGA GGAGCTGAGG CAGGCGGCCG AGATGGAGGA GCTACACCGC 
TCCTCCTGCT CTGAGTACTC ACCCTCACCC TCCCTTGACT CTGAGGCTGA GGCCTTGGAT 
GGTGGCCCTA GCCGGCTTTA CAAGTCAGGC AGTGAGTACA ACCTGCCCAC CTTCATGTCC 
CTCTACTCAC CAACCGAGAC ACCCTCCGGC AGCTCCACCA CTCCCAGTTC CGGACGGCCG 
CTCAAGAGCG CTGAGGAGGC TTATGAGGAG ATGATGCGCA AAGCTGAGCT GCTCCAGAGG 
CAGCAAGGCC AGGCAGCAGG GGCCCGGGGA CCCCATGGCG GCCCCTCTCA GCCCACAGGC 
CCCCGGGGCC TGGGCTCCTT CGAATATCAA GACACTACAG ACCGTGAGTA TGGCCAGGCT 
GCTCAGCCTG CCGCAGAGGG CACGCCAGCC AGCCTGGGAG CAGCCGTGTA CGAAGAAATC 
CTTCAGACAT CACAGAGCAT AGTCCGCATG CGGCAGGCCT CCTCACGAGA CCTGGCTTTT 
GCTGAGGACA AAAAGAAGGA GAAGCAGTTT CTAAATGCTG AGAGTGCATA CATGGACCCA 
ATGAAGCAAA ATGGTGGCCC CCTTACCCCT GGTACCAGTC CCACCCAGCT CGCTGCCCCT 
GTGTCCTTCT CTACCCCCAC CTCCTCAGAC AGCAGCGGGG GCCGAGTTAT TCCCGATGTC 
CGTGTCACTC AGCATTTTGC AAAGGAGACT CAGGACCCCC TCAAGCTGCA CAGCTCTCCT 
GCCTCCCCCA GCTCAGCCTC CAAGGAGATA GGCATGCCCT TTTCCCAGGG CCCTGGGACC 
CCAGCCACCA CAGCTGTGGC TCCTTGTCCA GCTGGGCTGC CACGAGGATA TATGACTCCA 
GCCTCCCCAG CAGGCTCCGA GCGTAGTCCT TCACCATCTT CCACAGCCCA CAGCTATGGA 
CACAGCCCAA CCACTGCAAA CTATGGGTCC CAAACTGAGG ATCTACCCCA GGCCCCCAGT 
GGCCTTGCTG CAGCTGGACG AGCTGCTAGA GAGAAGCCCT TGAGTGCGAG TGACGGTGAG 
GGTGGCACTC CTCAGCCTTC CCGGGCATAT TCCTACTTTG CAAGCTCCAG CCCACCTCTC 
TCCCCGTCTT CCCCCTCAGA GAGTCCCACA TTCTCCCCTG GCAAGATGGG CCCAAGGGCC 
ACAGCAGAGT TCTCTACACA GACGCCAAGT CCAGCCCCTG CCTCAGACAT GCCACGGAGC 
CCTGGTGCCC CCACTCCATC ACCTATGGTA GCCCAGGGTA CACAAACACC ACATCGACCC 
AGCACGCCTC GCCTGGTGTG GCAGGAGTCC TCTCAAGAGG CTCCCTTTAT GGTCATCACG 
CTGGCATCTG ACGCCTCCAG CCAGACCAGG ATGGTACATG CCAGTGCCTC CACCTCCCCG 
CTCTGCTCAC CTACTGAAAC CCAGCCCACC ACCCATGGCT ACAGCCAGAC AACACCTCCG 
AGTGTGTCTC AGCTGCCCCC AGAGCCACCT GGGCCACCTG GCTTTCCACG GGTGCCCAGT 
GCTGGTGCAG ATGGGCCCCT GGCACTATAT GGCTGGGGTG CCCTCCCTGC TGAGAACATC 
TCCCTGTGCC GGATCTCCTC TGTCCCTGGG ACGTCTAGGG TTGAGCCAGG CCCCAGGACC 
CCTGGCACTG CAGTGGTAGA CCTCCGTACA GCTGTCAAGC CCACTCCCAT CATCCTCACT 
GACCAGGGCA TGGACCTCAC CTCTCTTGCT GTGGAAGCGA GGAAGTATGG TCTTGCCCTG 
GATCCAATCC CAGGACGGCA GTCGACCGCC GTGCAGCCCT TGGTTATCAA CCTCAATGCC 
CAGGAGCATA CCTTCCTTGC TACTGCCACC ACCGTGAGCA TCACCATGGC CTCGTCTGTG 
TTCATGGCTC AACAAAAGCA GCCTGTGGTC TATGGAGACC CCTACCAGAG CCGCCTTGAC 
TTTGGCCAGG GTGGGGGTAG CCCTGTGTGC CTGGCCCAGG TCAAACAAGT AGAGCAGGCT 
GTCCAGACAG CCCCATACCG AAGTGGGCCC CGGGGAAGAC CCAGGGAGGC CAAGTTTGCC 
AGATATAACC TACCCAACCA AGTAGCTCCT CTGGCCAGAA GAGACGTTTT GATCACTCAG 
ATGGGCACCG CCCAGAGCAT TGGCCTCAAG CCAGGCCCAG TGCCAGAGCC AGGTGCCGAG 
CCCCACCGGG CCACCCCTGC AGAGCTGCGG TCACATGCTC TGCCAGGTGC CAGGAAGCCA 
CACACAGTGG TGGTGCAGAT GGGAGAGGGC ACAGCAGGCA CTGTGACCAC ACTGCTCCCA 
GAGGAGCCTG CGGGTGCCCT GGACCTTACC GGGATGAGGC CTGAGAGCCA GCTGGCATGC 
TGTGACATGG TCTACAAGCT CCCCTTTGGC AGCAGCTGCA CTGGCACCTT CCACCCGGCC 
CCCAGTGTGC CTGAGAAGAG CATGGCAGAT GCTGCCCCAC CTGGCCAAAG CAGCAGCCCC 
TTCTATGGTC CCCGGGACCC TGAGCCTCCT GAGCCCCCAA CCTACCGGGC ACAGGGGGTG 
GTGGGGCCTG GGCCCCATGA GGAGCAGAGG CCCTACCCAC AAGGCCTGCC TGGTAGGCTG 
TACTCCTCCA TGTCTGACAC CAATTTGGCT GAGGCTGGCC TCAACTACCA TGCCCAGAGG 
ATCGGGCAGC TCTTCCAGGG TCCTGGACGA GACTCGGCTA TGGACCTCAG CTCACTGAAG 
CACTCCTACA GCCTGGGCTT TGCGGATGGA CGCTACCTAG GGCAGGGCTT GCAGTATGGC 
TCAGTCACGG ACCTGCGTCA TCCTACAGAC CTTTTGGCTC ACCCGCTTCC CATGCGGCGC 
TATAGCTCAG TGTCGAACAT CTACTCAGAC CACAGGTACG GCCCACGGGG AGATGCAGTT 
GGCTTCCAGG AGGCCAGCCT GGCCCAGTAC AGTGCCACCA CAGCCCGTGA AATCAGTCGC 
ATGTGCGCTG CCCTCAACTC CATGGACCAG TATGGTGGGC GGCATGGCAG TGGTGGTGGT 
GGCCCTGACC TTGTGCAGTA CCAGCCCCAG CACGGGCCCG GGCTCAGTGC TCCACAGAGT 
CTGGTTCCCC TCAGACCTGG ACTCCTTGGT AACCCCACCT TTCCAGAGGG CCACCCAAGT 
CCTGGGAACT TGGCCCAGTA TGGGCCTGCA GCAGGCCAAG GAACAGCAGT CAGACAGCTG 
CTGCCGTCCA CAGCCACTGT ACGTGCAGCT GATGGCATGA TCTACTCGAC TATCAATACC 
CCAATTGCTG CAACACTGCC CATCACCACC CAGCCTGCCT CAGTCCTGCG GCCCATGGTG 
CGTGGTGGCA TGTACAGGCC TTACGCATCT GGTGGAATCA CAGCCGTGCC ACTCACCAGT 
CTGACACGTG TGCCCATGAT TGCCCCCCGG GTACCTCTTG GACCCACAGG GCTGTACCGC 
TATCCTGCAC CAAGTAGATT TCCCATTGCT TCCAGTGTTC CACCTGCAGA AGGGCCTGTC 
TATCTGGGGA AACCTGCTGC TGCCAAGGCC CCTGGGGCTG GGGGCCCTTC AAGGCCAGAG 
ATGCCAGTAG GGGCTGCACG GGAAGAGCCT CTTCCCACAA CCACCCCTGC TGCCATCAAG 
GAGGCTGCAG GAGCCCCAGC TCCTGCCCCA CTAGCTGGCC AGAAGCCACC AGCAGATGCT 
GCTCCTGGGG GTGGCAGTGG GGCCCTCAGC CGGCCAGGGT TCGAGAAAGA GGAAGCATCA 
CAGGAGGAGA GGCAGCGGAA GCAACAGGAG CAGCTGCTCC AGCTAGAGCG GGAGCGGGTG 
GAGTTGGAGA AGCTGCGACA ACTTCGGCTG CAAGAGGAGC TAGAGCGGGA ACGTGTGGAG 
CTGCAGAGGC ACCGTGAGGA GGAGCAGCTG CTGGTGCAGC GGGAGTTGCA GGAGCTGCAG 
ACCATCAAGC ACCATGTGCT GCAGCAGCAG CAAGAGGAAC GCCAGGCTCA ATTTGCACTG 
CAGCGGGAAC AGCTAGCGCA GCAGCGTCTG CAGCTGGAGC AGATCCAGCA GCTGCAGCAG 
CAGCTGCAGC AGCAGCTAGA GGAGCAGAAG CAGCGGCAGA AGGCTCCCTT TCCTGCAGCC 
TGTGAGGCAC CTGGCCGAGG GCCTCCCCTA GCGGCTGCTG AGTTGGCCCA GAATGGCCAG 
TATTGGCCCC CCCTTACACA TGCAGCCTTC ATTGCCATGG CAGGGCCTGA AGGACTTGGG 
CAGCCTCGTG AGCCTGTGCT GCACCGGGGT CTCCCCAGCT CTGCCTCAGA CATGTCACTG 
CAAACGGAGG AGCAGTGGGA GGCCAGCCGT AGTGGCATCA AGAAGCGGCA CTCCATGCCA 
CGCCTGCGGG ATGCCTGTGA GCTAGAGTCT GGGACTGAGC CCTGTGTGGT CAGGAGGATT 
GCCGACAGCA GCGTGCAGAC AGACGATGAG GATGGGGAGA GCCGCTACCT CTTGAGTCGG 
CGACGCCGGG CACGGCGGAG TGCTGACTGC AGTGTGCAGA CGGACGACGA AGACAGTGCT 
GAGTGGGAGC AGCCAGTGCG CCGCCGCAGG TCTCGTCTTC CCCGCCACTC AGACTCAGGC 
TCTGACAGCA AGCACGATGC CACTGCCTCA TCATCCAGTG CTGCTGCCAC TGTGAGGGCC 
ATGAGCAGCG TGGGCATCCA GACCATCAGT GACTGCTCCG TGCAGACGGA GCCTGACCAG 
CTGCCCAGGG TCTCTCCAGC CATCCACATC ACAGCTGCCA CCGATCCCAA GGTGGAGATC 
GTCAGGTACA TATCGGCGCC AGAGAAGACT GGGCGTGGGG AGAGCCTGGC CTGCCAGACG 
GAGCCAGATG GGCAGGCCCA GGGTGTAGCC GGGCCGCAGC TTGTAGGGCC AACTGCCATC 
AGCCCCTACC TGCCTGGCAT CCAGATCGTC ACCCCAGGGC CTCTGGGCAG ATTTGAAAAA 
AAGAAGCCAG ATCCCCTGGA GATTGGGTAC CAGGCCCACC TGCCTCCGGA GTCTCTCTCA 
CAGCTTGTGA GCCGCCAGCC TCCCAAGTCC CCTCAGGTCC TCTACTCACC AGTCTCACCC 
CTGTCCCCTC ACCGGCTCCT GGACACCTCC TTTGCTTCCA GTGAGAGGCT GAACAAAGCT 
CACGTGAGTC CCCAGAAGCA CTTCACGGCT GACAGCGCTC TCCGCCAGCA GACGCTGCCT 
CGCCCCATGA AGACCCTGCA GCGGTCCCTG TCTGACCCTA AGCCCCTCAG CCCCACCGCC 
GAAGAGTCTG CCAAAGAGAG ATTCTCCCTC TACCAGCACC AGGGGGGACT GGGTAGCCAG 
GTGTCGGCGT TGCCACCCAA CAGCCTGGTC CGCAAGGTGA AGCGGACACT GCCCAGCCCC 
CCTCCAGAGG AGGCTCACCT TCCCCTGGCT GGCCAGGCCT CCCCACAGCT GTATGCAGCC 
AGCCTGCTGC AGCGAGGGCT GACGGGGCCC ACCACTGTCC CTGCTACCAA GGCCAGCCTG 
CTCCGGGAGC TGGACCGGGA CCTGCGGCTG GTGGAGCATG AGTCCACCAA ACTGCGCAAG 
AAGCAGGCAG AGCTGGATGA GGAGGAGAAG GAGATTGACG CCAAGCTCAA GTACCTGGAG 
TTGGGTATCA CACAACGCAA AGAGTCTTTG GCCAAAGACC GGGGTGGCCG TGACTACCCA 
CCCTTGCGTG GTCTTGGCGA GCATCGTGAC TACCTATCGG ACAGTGAGCT CAACCAGCTG 
CGGCTCCAGG GCTGCACCAC TCCCGCTGGC CAGTTTGTGG ACTTCCCTGC CACTGCCGCT 
GCTCCTGCCA CCCCCTCTGG TCCCACTGCC TTCCAGCAGC CCCGCTTCCA GCCTCCAGCC 
CCACAGTATT CTGCAGGCAG TGGTGGGCCA ACTCAGAACG GATTCCCAGC CCACCAGGCC 
CCCACCTACC CTGGCCCCAG CACGTACCCA GCTCCTGCCT TTCCTCCTGG TGCCAGTTAC 
CCAGCTGAGC CTGGCCTGCC AAACCAGCAG GCTTTCCGCC CCACAGGCCA CTATGCAGGC 
CAAACACCCA TGCCAACCAC ACAGAGCACC CTTTTTCCAG TCCCCGCTGA TAGCCGTGCC 
CCACTGCAGA AGCCACGCCA GACATCGCTA GCCGACTTGG AGCAGAAGGT GCCCACCAAC 
TATGAGGTGA TCGCCAGCCC CGTTGTGCCC ATGTCTTCAG CCCCATCTGA AACCAGCTAC 
AGTGGCCCAG CAGTGAGCAG CGGCTATGAG CAGGGCAAGG TCCCTGAGGT GCCCCGGGCT 
GGTGACCGTG GCAGTGTGAG CCAGAGCCCA GCCCCCACCT ACCCCTCTGA CTCACACTAT 
ACCAGTCTGG AGCAGAACGT TCCTCGAAAC TACGTAATGA TTGATGACAT CAGTGAACTG 
ACCAAGGACA GCACCTCTAC TGCTCCTGAT AGCCAACGGC TGGAGCCCCT GGGGCCAGGC 
AGCAGTGGGC GTCCAGGGAA GGAGCCTGGA GAACCAGGTG TCCTTGACGG GCCCACACTG 
CCCTGCTGCT ATGCCAGAGG AGAAGAGGAA TCTGAGGAGG ACTCATACGA TCCCCGCGGG 
AAGGGTGGCC ACCTCCGGAG CATGGAGAGC AATGGTCGAC CAGCCAGTAC CCACTACTAT 
GGTGACAGTG ACTACAGGCA TGGGGCTCGA GTAGAGAAGT ATGGTCCAGG GCCCATGGGG 
CCCAAGCATC CCTCCAAGAG CCTGGCTCCA GCTGCCATCT CCTCAAAGCG CAGCAAGCAC 
CGGAAGCAGG GCATGGAGCA AAAGATATCC AAGTTCTCGC CTATTGAAGA GGCCAAAGAC 
GTAGAGTCAG ACCTGGCGTC CTACCCCCCA CCTGCAGTCA GCAGCAGCCT GGTCTCTCGG 
GGCAGGAAGT TCCAGGATGA AATCACCTAT GGGCTCAAGA AGAACGTGTA TGAGCAGCAA 
AAATACTATG GGATGTCCAG CCGGGACGCA GTGGAGGACG ACCGCATTTA TGGCGGGAGC 
AGCCGGTCCC GGGCACCTTC TGCATACAGT GGGGAGAAGC TGTCCAGCCA CGACTTCAGT 
GGCTGGGGCA AGGGGTACGA AAGGGAACGG GAGGCTGTGG AGCGACTTCA AAAAGCGGGC 
CCCAAGCCCT CATCCCTAAG TATGGCCCAC AGCCGGGTAC GACCCCCCAT GCGGAGCCAG 
GCCTCTGAAG AGGAGAGCCC CGTCAGTCCT TTGGGGAGGC CCCGCCCTGC CGGAGGGCCC 
CTCCCTCCCG GCGGGGATAC CTGCCCACAG TTCTGCTCCA GCCACTCCAT GCCTGATGTC 
CAGGAACATG TCAAGGACGG ACCTCGGGCC CACGCATATA AGCGTGAGGA GGGCTACATC 
CTGGATGATT CCCATTGCGT GGTTTCCGAC AGCGAAGCGT ATCACCTGGG CCAGGAGGAG 
ACGGACTGGT TTGATAAGCC CCGGGATGCC CGCTCTGACC GGTTCAGGCA CCACGGGGGC 
CATGCAGTTT CCTCCTCCTC CCAGAAGCGA GGCCCTGCCA GGCACAGCTA CCATGACTAC 
GATGAACCCC CTGAGGAGGG CCTGTGGCCT CATGATGAGG GTGGCCCAGG CCGCCATGCC 
TCAGCCAAGG AACACCGGCA CGGTGACCAC GGGCGGCACT CAGGCCGCCA CACTGGTGAG 
GAGCCGGGAC GGCGTGCTGC CAAACCACAC GCTCGGGACC TGGGTCGCCA TGAGGCCCGG 
CCCCACTCTC AGCCCAGCTC TGCTCCAGCT ATGCCGAAGA AGGGTCAGCC TGGGTATCCC 
AGCTCTGCTG AGTACTCACA GCCATCCCGT GCTTCATCCG CATACCATCA TGCCTCTGAC 
AGCAAGAAGG GCTCCCGGCA AGCCCACTCC GGGCCCGCTG CACTGCAGTC AAAGGCAGAA 
CCCCAGGCGC AGCCGCAGCT GCAAGGTCGG CAGGCAGCTC CAGGACCACA GCAGTCACAG 
TCACCATCAT CCAGGCAAAT ACCCTCTGGG GCAGCATCAC GCCAGCCACA GACACAGCAG 
CAGCAGCAAG GTCTTGGGCT GCAGCCCCCA CAGCAGGCTC TGACACAGGC TCGGCTGCAG 
CAACAGAGCC AGCCAACCAC CCGGGGCTCA GCCCCTGCTG CCAGCCAGCC TGCAGGGAAG 
CCTCAGCCAG GCCCCAGCAC AGCCACAGGT CCTCAACCAG CAGGACCGCC ACGGGCAGAA 
CAGACAAATG GCTCTAAAGG GACAGCCAAA GCACCGCAAC AGGGGAGGGC TCCTCAGGCC 
CAGCCAGCAC CAGGACCTGG ACCTGCAGGT GTGAAGGCTG GAGCCAGGCC TGGAGGAACC 
CCAGGGGCTC CCGCCGGCCA GCCAGGTGCC GATGGGGAGA GCGTGTTCTC CAAGATCCTC 
CCTGGCGGGG CAGCCGAGCA AGCTGGCAAA CTGACGGAAG CTGTCTCTGC TTTTGGCAAA 
AAATTTTCCT CATTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003449.2
CDS	115..11895
Translation	MGNEVSLEGGAGDGPLPPGGAGPGPGPGPGPGAGKPPSAPAGGGQLPAAGAARSTAVPPVPGPGPGPGPGPGPGSTSRRLDPKEPLGNQRAASPTPKQASATTPGHESPRETRAQGPAGQEADGPRRTLQVDSRTQRSGRSPSVSPDRGSTPTSPYSVPQIAPLPSSTLCPICKTSDLTSTPSQPNFNTCTQCHNKVCNQCGFNPNPHLTQVKEWLCLNCQMQRALGMDMTTAPRSKSQQQLHSPALSPAHSPAKQPLGKPDQERSRGPGGPQPGSRQAETARATSVPGPAQAAAPPEVGRVSPQPPQPTKPSTAEPRPPAGEAPAKSATAVPAGLGATEQTQEGLTGKLFGLGASLLTQASTLMSVQPEADTQGQPAPSKGTPKIVFNDASKEAGPKPLGSGPGPGPAPGAKTEPGARMGPGSGPGALPKTGGTTSPKHGRAEHQAASKAAAKPKTMPKERAICPLCQAELNVGSKSPANYNTCTTCRLQVCNLCGFNPTPHLVEKTEWLCLNCQTKRLLEGSLGEPTPLPPPTSQQPPVGAPHRASGTSPLKQKGPQGLGQPSGPLPAKASPLSTKASPLPSKASPQAKPLRASEPSKTPSSVQEKKTRVPTKAEPMPKPPPETTPTPATPKVKSGVRRAEPATPVVKAVPEAPKGGEAEDLVGKPYSQDASRSPQSLSDTGYSSDGISSSQSEITGVVQQEVEQLDSAGVTGPHPPSPSEIHKVGSSMRPLLQAQGLAPSERSKPLSSGTGEEQKQRPHSLSITPEAFDSDEELEDILEEDEDSAEWRRRREQQDTAESSDDFGSQLRHDYVEDSSEGGLSPLPPQPPARAAELTDEDFMRRQILEMSAEEDNLEEDDTATSGRGLAKHGTQKGGPRPRPEPSQEPAALPKRRLPHNATTGYEELLPEGGSAEATDGSGTLQGGLRRFKTIELNSTGSYGHELDLGQGPDPSLDREPELEMESLTGSPEDRSRGEHSSTLPASTPSYTSGTSPTSLSSLEEDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLREQEKMREVEQQRIRSTARKTRRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSPSLDSEAEALDGGPSRLYKSGSEYNLPTFMSLYSPTETPSGSSTTPSSGRPLKSAEEAYEEMMRKAELLQRQQGQAAGARGPHGGPSQPTGPRGLGSFEYQDTTDREYGQAAQPAAEGTPASLGAAVYEEILQTSQSIVRMRQASSRDLAFAEDKKKEKQFLNAESAYMDPMKQNGGPLTPGTSPTQLAAPVSFSTPTSSDSSGGRVIPDVRVTQHFAKETQDPLKLHSSPASPSSASKEIGMPFSQGPGTPATTAVAPCPAGLPRGYMTPASPAGSERSPSPSSTAHSYGHSPTTANYGSQTEDLPQAPSGLAAAGRAAREKPLSASDGEGGTPQPSRAYSYFASSSPPLSPSSPSESPTFSPGKMGPRATAEFSTQTPSPAPASDMPRSPGAPTPSPMVAQGTQTPHRPSTPRLVWQESSQEAPFMVITLASDASSQTRMVHASASTSPLCSPTETQPTTHGYSQTTPPSVSQLPPEPPGPPGFPRVPSAGADGPLALYGWGALPAENISLCRISSVPGTSRVEPGPRTPGTAVVDLRTAVKPTPIILTDQGMDLTSLAVEARKYGLALDPIPGRQSTAVQPLVINLNAQEHTFLATATTVSITMASSVFMAQQKQPVVYGDPYQSRLDFGQGGGSPVCLAQVKQVEQAVQTAPYRSGPRGRPREAKFARYNLPNQVAPLARRDVLITQMGTAQSIGLKPGPVPEPGAEPHRATPAELRSHALPGARKPHTVVVQMGEGTAGTVTTLLPEEPAGALDLTGMRPESQLACCDMVYKLPFGSSCTGTFHPAPSVPEKSMADAAPPGQSSSPFYGPRDPEPPEPPTYRAQGVVGPGPHEEQRPYPQGLPGRLYSSMSDTNLAEAGLNYHAQRIGQLFQGPGRDSAMDLSSLKHSYSLGFADGRYLGQGLQYGSVTDLRHPTDLLAHPLPMRRYSSVSNIYSDHRYGPRGDAVGFQEASLAQYSATTAREISRMCAALNSMDQYGGRHGSGGGGPDLVQYQPQHGPGLSAPQSLVPLRPGLLGNPTFPEGHPSPGNLAQYGPAAGQGTAVRQLLPSTATVRAADGMIYSTINTPIAATLPITTQPASVLRPMVRGGMYRPYASGGITAVPLTSLTRVPMIAPRVPLGPTGLYRYPAPSRFPIASSVPPAEGPVYLGKPAAAKAPGAGGPSRPEMPVGAAREEPLPTTTPAAIKEAAGAPAPAPLAGQKPPADAAPGGGSGALSRPGFEKEEASQEERQRKQQEQLLQLERERVELEKLRQLRLQEELERERVELQRHREEEQLLVQRELQELQTIKHHVLQQQQEERQAQFALQREQLAQQRLQLEQIQQLQQQLQQQLEEQKQRQKAPFPAACEAPGRGPPLAAAELAQNGQYWPPLTHAAFIAMAGPEGLGQPREPVLHRGLPSSASDMSLQTEEQWEASRSGIKKRHSMPRLRDACELESGTEPCVVRRIADSSVQTDDEDGESRYLLSRRRRARRSADCSVQTDDEDSAEWEQPVRRRRSRLPRHSDSGSDSKHDATASSSSAAATVRAMSSVGIQTISDCSVQTEPDQLPRVSPAIHITAATDPKVEIVRYISAPEKTGRGESLACQTEPDGQAQGVAGPQLVGPTAISPYLPGIQIVTPGPLGRFEKKKPDPLEIGYQAHLPPESLSQLVSRQPPKSPQVLYSPVSPLSPHRLLDTSFASSERLNKAHVSPQKHFTADSALRQQTLPRPMKTLQRSLSDPKPLSPTAEESAKERFSLYQHQGGLGSQVSALPPNSLVRKVKRTLPSPPPEEAHLPLAGQASPQLYAASLLQRGLTGPTTVPATKASLLRELDRDLRLVEHESTKLRKKQAELDEEEKEIDAKLKYLELGITQRKESLAKDRGGRDYPPLRGLGEHRDYLSDSELNQLRLQGCTTPAGQFVDFPATAAAPATPSGPTAFQQPRFQPPAPQYSAGSGGPTQNGFPAHQAPTYPGPSTYPAPAFPPGASYPAEPGLPNQQAFRPTGHYAGQTPMPTTQSTLFPVPADSRAPLQKPRQTSLADLEQKVPTNYEVIASPVVPMSSAPSETSYSGPAVSSGYEQGKVPEVPRAGDRGSVSQSPAPTYPSDSHYTSLEQNVPRNYVMIDDISELTKDSTSTAPDSQRLEPLGPGSSGRPGKEPGEPGVLDGPTLPCCYARGEEESEEDSYDPRGKGGHLRSMESNGRPASTHYYGDSDYRHGARVEKYGPGPMGPKHPSKSLAPAAISSKRSKHRKQGMEQKISKFSPIEEAKDVESDLASYPPPAVSSSLVSRGRKFQDEITYGLKKNVYEQQKYYGMSSRDAVEDDRIYGGSSRSRAPSAYSGEKLSSHDFSGWGKGYEREREAVERLQKAGPKPSSLSMAHSRVRPPMRSQASEEESPVSPLGRPRPAGGPLPPGGDTCPQFCSSHSMPDVQEHVKDGPRAHAYKREEGYILDDSHCVVSDSEAYHLGQEETDWFDKPRDARSDRFRHHGGHAVSSSSQKRGPARHSYHDYDEPPEEGLWPHDEGGPGRHASAKEHRHGDHGRHSGRHTGEEPGRRAAKPHARDLGRHEARPHSQPSSAPAMPKKGQPGYPSSAEYSQPSRASSAYHHASDSKKGSRQAHSGPAALQSKAEPQAQPQLQGRQAAPGPQQSQSPSSRQIPSGAASRQPQTQQQQQGLGLQPPQQALTQARLQQQSQPTTRGSAPAASQPAGKPQPGPSTATGPQPAGPPRAEQTNGSKGTAKAPQQGRAPQAQPAPGPGPAGVKAGARPGGTPGAPAGQPGADGESVFSKILPGGAAEQAGKLTEAVSAFGKKFSSFW

Target ORF information:

RefSeq Version	NM_003458.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bassoon presynaptic cytomatrix protein (BSN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003458.3

ORF Insert Sequence:

ATGGGCAACG AGGTCAGCCT GGAGGGCGGC GCTGGCGACG GGCCGCTGCC GCCCGGCGGC 
GCCGGCCCCG GCCCGGGCCC CGGCCCCGGC CCCGGCGCAG GAAAGCCGCC TTCAGCACCG 
GCCGGTGGCG GACAGCTCCC CGCGGCGGGA GCAGCGCGGT CGACCGCGGT ACCACCGGTC 
CCTGGCCCCG GCCCCGGCCC CGGTCCCGGC CCTGGCCCGG GCAGCACTTC CCGGAGACTG 
GACCCCAAGG AACCCCTGGG TAACCAGAGA GCAGCTTCCC CAACTCCGAA GCAGGCTTCT 
GCTACCACTC CTGGCCATGA GAGCCCCCGA GAGACAAGGG CACAGGGACC AGCAGGCCAG 
GAGGCTGATG GTCCCCGCAG GACGCTGCAG GTAGACAGCA GGACACAGAG ATCAGGGCGG 
TCCCCCTCAG TGTCACCGGA CAGAGGCAGC ACCCCCACAT CACCCTACTC CGTCCCTCAG 
ATCGCCCCCC TTCCCAGCAG CACGCTGTGT CCCATATGCA AGACTTCGGA CCTCACGTCG 
ACCCCCAGCC AGCCAAACTT CAACACCTGC ACCCAGTGTC ACAACAAGGT CTGCAACCAG 
TGTGGGTTCA ACCCCAACCC TCATCTCACC CAGGTGAAGG AGTGGCTCTG TCTGAACTGT 
CAGATGCAGA GGGCTCTGGG AATGGACATG ACCACTGCAC CTCGCTCCAA GAGCCAGCAG 
CAGCTGCACT CCCCAGCCCT GTCTCCTGCC CACTCCCCGG CCAAACAGCC CCTGGGGAAG 
CCAGACCAAG AGAGATCTCG GGGCCCAGGA GGACCACAGC CTGGGTCCCG CCAGGCTGAG 
ACAGCCAGGG CCACCTCAGT GCCGGGGCCT GCCCAAGCAG CTGCCCCTCC AGAGGTGGGG 
AGGGTGTCTC CTCAGCCCCC TCAACCCACC AAGCCTTCCA CAGCTGAGCC CAGGCCACCT 
GCAGGAGAGG CCCCGGCCAA AAGTGCCACC GCAGTGCCCG CTGGGCTTGG TGCCACTGAG 
CAGACCCAGG AGGGCCTCAC TGGTAAGCTC TTCGGCCTTG GCGCGTCACT GCTAACCCAG 
GCGAGCACCC TCATGTCTGT GCAGCCCGAG GCTGACACCC AGGGCCAGCC TGCCCCCAGC 
AAGGGGACAC CTAAGATCGT CTTCAATGAT GCCAGCAAGG AGGCTGGCCC AAAACCCTTG 
GGCTCAGGGC CCGGGCCTGG GCCAGCACCT GGAGCCAAAA CTGAGCCTGG GGCTAGAATG 
GGTCCTGGAT CTGGACCTGG AGCCCTGCCG AAAACTGGGG GAACAACCAG TCCAAAGCAT 
GGCAGAGCAG AACATCAGGC AGCATCGAAG GCTGCTGCCA AGCCAAAGAC CATGCCGAAG 
GAAAGGGCCA TCTGCCCACT GTGCCAAGCC GAGCTCAACG TGGGCAGCAA GAGCCCAGCC 
AACTATAACA CATGCACCAC CTGCAGGCTC CAGGTGTGCA ACCTGTGTGG CTTCAACCCA 
ACACCCCACC TGGTGGAGAA AACAGAGTGG CTCTGTCTGA ACTGCCAAAC CAAGCGGCTA 
CTGGAGGGCA GCCTAGGAGA GCCGACCCCC CTGCCGCCGC CCACCTCACA GCAGCCCCCT 
GTAGGGGCCC CTCACCGTGC ATCTGGAACA TCCCCTCTGA AGCAGAAAGG GCCACAGGGG 
CTGGGCCAGC CTTCAGGCCC CCTGCCTGCC AAGGCCAGCC CTCTATCCAC CAAGGCCAGC 
CCTCTGCCCA GCAAGGCCAG CCCCCAGGCC AAGCCCCTCA GGGCTTCTGA ACCCAGCAAG 
ACCCCAAGCA GTGTCCAGGA AAAGAAGACC CGAGTCCCCA CTAAAGCTGA GCCCATGCCG 
AAGCCACCTC CAGAGACTAC CCCAACCCCT GCGACTCCTA AAGTAAAGAG TGGGGTGAGG 
AGGGCTGAAC CTGCCACCCC TGTCGTCAAG GCTGTTCCAG AAGCCCCCAA GGGTGGGGAG 
GCGGAGGACC TGGTGGGCAA GCCTTACTCT CAGGATGCGT CTCGGAGCCC ACAGAGCCTC 
AGTGACACAG GCTATTCCTC TGACGGCATC TCTAGCTCCC AGAGTGAGAT CACAGGAGTC 
GTGCAGCAGG AGGTGGAACA GCTGGACAGT GCAGGGGTGA CAGGGCCACA TCCACCCAGC 
CCCTCCGAGA TCCACAAGGT GGGGAGCAGC ATGCGGCCTT TGCTGCAGGC CCAGGGCCTG 
GCCCCAAGTG AGCGGAGCAA GCCACTCTCC AGCGGTACTG GCGAGGAGCA GAAGCAGCGG 
CCCCACTCCT TGTCCATCAC GCCTGAGGCC TTTGACTCTG ATGAGGAGCT GGAGGATATC 
CTGGAGGAAG ACGAAGACTC TGCTGAGTGG AGGCGCCGGA GAGAGCAGCA GGACACTGCC 
GAGTCCTCAG ACGACTTTGG CAGCCAATTG AGGCACGACT ATGTGGAGGA CAGCAGTGAG 
GGTGGCCTGT CCCCTCTTCC ACCCCAGCCC CCAGCCCGGG CAGCAGAACT GACTGATGAG 
GATTTCATGC GACGGCAGAT TCTCGAGATG AGCGCCGAGG AAGACAACCT GGAGGAGGAT 
GACACTGCCA CCTCCGGGCG TGGCCTGGCC AAACATGGCA CCCAGAAAGG TGGCCCCAGA 
CCCAGGCCTG AGCCTAGCCA AGAACCAGCA GCACTGCCCA AGAGGCGCCT GCCCCACAAT 
GCCACCACGG GCTATGAGGA GCTGCTCCCT GAGGGAGGCT CAGCAGAGGC TACCGATGGC 
AGTGGGACCC TGCAGGGTGG GCTCCGTCGC TTCAAGACCA TTGAGCTCAA CAGCACGGGA 
AGTTATGGTC ATGAGTTGGA CCTGGGCCAA GGCCCAGACC CCAGTCTGGA CCGGGAGCCT 
GAGCTGGAGA TGGAGAGCCT AACGGGCTCC CCTGAGGACC GCTCCCGTGG TGAGCACTCC 
TCTACATTGC CTGCCTCCAC ACCCAGCTAC ACCTCGGGCA CCTCTCCCAC CTCTCTGTCC 
TCCCTAGAGG AGGACAGTGA CAGCAGCCCC AGCCGCAGGC AGCGTCTAGA AGAAGCAAAG 
CAGCAGCGCA AGGCCCGGCA CCGCTCCCAC GGGCCCCTGC TACCCACCAT CGAGGACTCC 
TCAGAGGAGG AGGAGCTGCG GGAGGAAGAG GAGCTGCTTC GTGAGCAAGA GAAGATGCGG 
GAGGTGGAGC AGCAGCGCAT CCGCAGCACG GCCCGCAAGA CCCGGCGGGA CAAGGAAGAA 
CTGCGGGCCC AGCGGAGGCG AGAGCGCTCC AAGACACCAC CCAGTAACTT GTCACCCATC 
GAGGATGCCT CCCCGACGGA GGAGCTGAGG CAGGCGGCCG AGATGGAGGA GCTACACCGC 
TCCTCCTGCT CTGAGTACTC ACCCTCACCC TCCCTTGACT CTGAGGCTGA GGCCTTGGAT 
GGTGGCCCTA GCCGGCTTTA CAAGTCAGGC AGTGAGTACA ACCTGCCCAC CTTCATGTCC 
CTCTACTCAC CAACCGAGAC ACCCTCCGGC AGCTCCACCA CTCCCAGTTC CGGACGGCCG 
CTCAAGAGCG CTGAGGAGGC TTATGAGGAG ATGATGCGCA AAGCTGAGCT GCTCCAGAGG 
CAGCAAGGCC AGGCAGCAGG GGCCCGGGGA CCCCATGGCG GCCCCTCTCA GCCCACAGGC 
CCCCGGGGCC TGGGCTCCTT CGAATATCAA GACACTACAG ACCGTGAGTA TGGCCAGGCT 
GCTCAGCCTG CCGCAGAGGG CACGCCAGCC AGCCTGGGAG CAGCCGTGTA CGAAGAAATC 
CTTCAGACAT CACAGAGCAT AGTCCGCATG CGGCAGGCCT CCTCACGAGA CCTGGCTTTT 
GCTGAGGACA AAAAGAAGGA GAAGCAGTTT CTAAATGCTG AGAGTGCATA CATGGACCCA 
ATGAAGCAAA ATGGTGGCCC CCTTACCCCT GGTACCAGTC CCACCCAGCT CGCTGCCCCT 
GTGTCCTTCT CTACCCCCAC CTCCTCAGAC AGCAGCGGGG GCCGAGTTAT TCCCGATGTC 
CGTGTCACTC AGCATTTTGC AAAGGAGACT CAGGACCCCC TCAAGCTGCA CAGCTCTCCT 
GCCTCCCCCA GCTCAGCCTC CAAGGAGATA GGCATGCCCT TTTCCCAGGG CCCTGGGACC 
CCAGCCACCA CAGCTGTGGC TCCTTGTCCA GCTGGGCTGC CACGAGGATA TATGACTCCA 
GCCTCCCCAG CAGGCTCCGA GCGTAGTCCT TCACCATCTT CCACAGCCCA CAGCTATGGA 
CACAGCCCAA CCACTGCAAA CTATGGGTCC CAAACTGAGG ATCTACCCCA GGCCCCCAGT 
GGCCTTGCTG CAGCTGGACG AGCTGCTAGA GAGAAGCCCT TGAGTGCGAG TGACGGTGAG 
GGTGGCACTC CTCAGCCTTC CCGGGCATAT TCCTACTTTG CAAGCTCCAG CCCACCTCTC 
TCCCCGTCTT CCCCCTCAGA GAGTCCCACA TTCTCCCCTG GCAAGATGGG CCCAAGGGCC 
ACAGCAGAGT TCTCTACACA GACGCCAAGT CCAGCCCCTG CCTCAGACAT GCCACGGAGC 
CCTGGTGCCC CCACTCCATC ACCTATGGTA GCCCAGGGTA CACAAACACC ACATCGACCC 
AGCACGCCTC GCCTGGTGTG GCAGGAGTCC TCTCAAGAGG CTCCCTTTAT GGTCATCACG 
CTGGCATCTG ACGCCTCCAG CCAGACCAGG ATGGTACATG CCAGTGCCTC CACCTCCCCG 
CTCTGCTCAC CTACTGAAAC CCAGCCCACC ACCCATGGCT ACAGCCAGAC AACACCTCCG 
AGTGTGTCTC AGCTGCCCCC AGAGCCACCT GGGCCACCTG GCTTTCCACG GGTGCCCAGT 
GCTGGTGCAG ATGGGCCCCT GGCACTATAT GGCTGGGGTG CCCTCCCTGC TGAGAACATC 
TCCCTGTGCC GGATCTCCTC TGTCCCTGGG ACGTCTAGGG TTGAGCCAGG CCCCAGGACC 
CCTGGCACTG CAGTGGTAGA CCTCCGTACA GCTGTCAAGC CCACTCCCAT CATCCTCACT 
GACCAGGGCA TGGACCTCAC CTCTCTTGCT GTGGAAGCGA GGAAGTATGG TCTTGCCCTG 
GATCCAATCC CAGGACGGCA GTCGACCGCC GTGCAGCCCT TGGTTATCAA CCTCAATGCC 
CAGGAGCATA CCTTCCTTGC TACTGCCACC ACCGTGAGCA TCACCATGGC CTCGTCTGTG 
TTCATGGCTC AACAAAAGCA GCCTGTGGTC TATGGAGACC CCTACCAGAG CCGCCTTGAC 
TTTGGCCAGG GTGGGGGTAG CCCTGTGTGC CTGGCCCAGG TCAAACAAGT AGAGCAGGCT 
GTCCAGACAG CCCCATACCG AAGTGGGCCC CGGGGAAGAC CCAGGGAGGC CAAGTTTGCC 
AGATATAACC TACCCAACCA AGTAGCTCCT CTGGCCAGAA GAGACGTTTT GATCACTCAG 
ATGGGCACCG CCCAGAGCAT TGGCCTCAAG CCAGGCCCAG TGCCAGAGCC AGGTGCCGAG 
CCCCACCGGG CCACCCCTGC AGAGCTGCGG TCACATGCTC TGCCAGGTGC CAGGAAGCCA 
CACACAGTGG TGGTGCAGAT GGGAGAGGGC ACAGCAGGCA CTGTGACCAC ACTGCTCCCA 
GAGGAGCCTG CGGGTGCCCT GGACCTTACC GGGATGAGGC CTGAGAGCCA GCTGGCATGC 
TGTGACATGG TCTACAAGCT CCCCTTTGGC AGCAGCTGCA CTGGCACCTT CCACCCGGCC 
CCCAGTGTGC CTGAGAAGAG CATGGCAGAT GCTGCCCCAC CTGGCCAAAG CAGCAGCCCC 
TTCTATGGTC CCCGGGACCC TGAGCCTCCT GAGCCCCCAA CCTACCGGGC ACAGGGGGTG 
GTGGGGCCTG GGCCCCATGA GGAGCAGAGG CCCTACCCAC AAGGCCTGCC TGGTAGGCTG 
TACTCCTCCA TGTCTGACAC CAATTTGGCT GAGGCTGGCC TCAACTACCA TGCCCAGAGG 
ATCGGGCAGC TCTTCCAGGG TCCTGGACGA GACTCGGCTA TGGACCTCAG CTCACTGAAG 
CACTCCTACA GCCTGGGCTT TGCGGATGGA CGCTACCTAG GGCAGGGCTT GCAGTATGGC 
TCAGTCACGG ACCTGCGTCA TCCTACAGAC CTTTTGGCTC ACCCGCTTCC CATGCGGCGC 
TATAGCTCAG TGTCGAACAT CTACTCAGAC CACAGGTACG GCCCACGGGG AGATGCAGTT 
GGCTTCCAGG AGGCCAGCCT GGCCCAGTAC AGTGCCACCA CAGCCCGTGA AATCAGTCGC 
ATGTGCGCTG CCCTCAACTC CATGGACCAG TATGGTGGGC GGCATGGCAG TGGTGGTGGT 
GGCCCTGACC TTGTGCAGTA CCAGCCCCAG CACGGGCCCG GGCTCAGTGC TCCACAGAGT 
CTGGTTCCCC TCAGACCTGG ACTCCTTGGT AACCCCACCT TTCCAGAGGG CCACCCAAGT 
CCTGGGAACT TGGCCCAGTA TGGGCCTGCA GCAGGCCAAG GAACAGCAGT CAGACAGCTG 
CTGCCGTCCA CAGCCACTGT ACGTGCAGCT GATGGCATGA TCTACTCGAC TATCAATACC 
CCAATTGCTG CAACACTGCC CATCACCACC CAGCCTGCCT CAGTCCTGCG GCCCATGGTG 
CGTGGTGGCA TGTACAGGCC TTACGCATCT GGTGGAATCA CAGCCGTGCC ACTCACCAGT 
CTGACACGTG TGCCCATGAT TGCCCCCCGG GTACCTCTTG GACCCACAGG GCTGTACCGC 
TATCCTGCAC CAAGTAGATT TCCCATTGCT TCCAGTGTTC CACCTGCAGA AGGGCCTGTC 
TATCTGGGGA AACCTGCTGC TGCCAAGGCC CCTGGGGCTG GGGGCCCTTC AAGGCCAGAG 
ATGCCAGTAG GGGCTGCACG GGAAGAGCCT CTTCCCACAA CCACCCCTGC TGCCATCAAG 
GAGGCTGCAG GAGCCCCAGC TCCTGCCCCA CTAGCTGGCC AGAAGCCACC AGCAGATGCT 
GCTCCTGGGG GTGGCAGTGG GGCCCTCAGC CGGCCAGGGT TCGAGAAAGA GGAAGCATCA 
CAGGAGGAGA GGCAGCGGAA GCAACAGGAG CAGCTGCTCC AGCTAGAGCG GGAGCGGGTG 
GAGTTGGAGA AGCTGCGACA ACTTCGGCTG CAAGAGGAGC TAGAGCGGGA ACGTGTGGAG 
CTGCAGAGGC ACCGTGAGGA GGAGCAGCTG CTGGTGCAGC GGGAGTTGCA GGAGCTGCAG 
ACCATCAAGC ACCATGTGCT GCAGCAGCAG CAAGAGGAAC GCCAGGCTCA ATTTGCACTG 
CAGCGGGAAC AGCTAGCGCA GCAGCGTCTG CAGCTGGAGC AGATCCAGCA GCTGCAGCAG 
CAGCTGCAGC AGCAGCTAGA GGAGCAGAAG CAGCGGCAGA AGGCTCCCTT TCCTGCAGCC 
TGTGAGGCAC CTGGCCGAGG GCCTCCCCTA GCGGCTGCTG AGTTGGCCCA GAATGGCCAG 
TATTGGCCCC CCCTTACACA TGCAGCCTTC ATTGCCATGG CAGGGCCTGA AGGACTTGGG 
CAGCCTCGTG AGCCTGTGCT GCACCGGGGT CTCCCCAGCT CTGCCTCAGA CATGTCACTG 
CAAACGGAGG AGCAGTGGGA GGCCAGCCGT AGTGGCATCA AGAAGCGGCA CTCCATGCCA 
CGCCTGCGGG ATGCCTGTGA GCTAGAGTCT GGGACTGAGC CCTGTGTGGT CAGGAGGATT 
GCCGACAGCA GCGTGCAGAC AGACGATGAG GATGGGGAGA GCCGCTACCT CTTGAGTCGG 
CGACGCCGGG CACGGCGGAG TGCTGACTGC AGTGTGCAGA CGGACGACGA AGACAGTGCT 
GAGTGGGAGC AGCCAGTGCG CCGCCGCAGG TCTCGTCTTC CCCGCCACTC AGACTCAGGC 
TCTGACAGCA AGCACGATGC CACTGCCTCA TCATCCAGTG CTGCTGCCAC TGTGAGGGCC 
ATGAGCAGCG TGGGCATCCA GACCATCAGT GACTGCTCCG TGCAGACGGA GCCTGACCAG 
CTGCCCAGGG TCTCTCCAGC CATCCACATC ACAGCTGCCA CCGATCCCAA GGTGGAGATC 
GTCAGGTACA TATCGGCGCC AGAGAAGACT GGGCGTGGGG AGAGCCTGGC CTGCCAGACG 
GAGCCAGATG GGCAGGCCCA GGGTGTAGCC GGGCCGCAGC TTGTAGGGCC AACTGCCATC 
AGCCCCTACC TGCCTGGCAT CCAGATCGTC ACCCCAGGGC CTCTGGGCAG ATTTGAAAAA 
AAGAAGCCAG ATCCCCTGGA GATTGGGTAC CAGGCCCACC TGCCTCCGGA GTCTCTCTCA 
CAGCTTGTGA GCCGCCAGCC TCCCAAGTCC CCTCAGGTCC TCTACTCACC AGTCTCACCC 
CTGTCCCCTC ACCGGCTCCT GGACACCTCC TTTGCTTCCA GTGAGAGGCT GAACAAAGCT 
CACGTGAGTC CCCAGAAGCA CTTCACGGCT GACAGCGCTC TCCGCCAGCA GACGCTGCCT 
CGCCCCATGA AGACCCTGCA GCGGTCCCTG TCTGACCCTA AGCCCCTCAG CCCCACCGCC 
GAAGAGTCTG CCAAAGAGAG ATTCTCCCTC TACCAGCACC AGGGGGGACT GGGTAGCCAG 
GTGTCGGCGT TGCCACCCAA CAGCCTGGTC CGCAAGGTGA AGCGGACACT GCCCAGCCCC 
CCTCCAGAGG AGGCTCACCT TCCCCTGGCT GGCCAGGCCT CCCCACAGCT GTATGCAGCC 
AGCCTGCTGC AGCGAGGGCT GACGGGGCCC ACCACTGTCC CTGCTACCAA GGCCAGCCTG 
CTCCGGGAGC TGGACCGGGA CCTGCGGCTG GTGGAGCATG AGTCCACCAA ACTGCGCAAG 
AAGCAGGCAG AGCTGGATGA GGAGGAGAAG GAGATTGACG CCAAGCTCAA GTACCTGGAG 
TTGGGTATCA CACAACGCAA AGAGTCTTTG GCCAAAGACC GGGGTGGCCG TGACTACCCA 
CCCTTGCGTG GTCTTGGCGA GCATCGTGAC TACCTATCGG ACAGTGAGCT CAACCAGCTG 
CGGCTCCAGG GCTGCACCAC TCCCGCTGGC CAGTTTGTGG ACTTCCCTGC CACTGCCGCT 
GCTCCTGCCA CCCCCTCTGG TCCCACTGCC TTCCAGCAGC CCCGCTTCCA GCCTCCAGCC 
CCACAGTATT CTGCAGGCAG TGGTGGGCCA ACTCAGAACG GATTCCCAGC CCACCAGGCC 
CCCACCTACC CTGGCCCCAG CACGTACCCA GCTCCTGCCT TTCCTCCTGG TGCCAGTTAC 
CCAGCTGAGC CTGGCCTGCC AAACCAGCAG GCTTTCCGCC CCACAGGCCA CTATGCAGGC 
CAAACACCCA TGCCAACCAC ACAGAGCACC CTTTTTCCAG TCCCCGCTGA TAGCCGTGCC 
CCACTGCAGA AGCCACGCCA GACATCGCTA GCCGACTTGG AGCAGAAGGT GCCCACCAAC 
TATGAGGTGA TCGCCAGCCC CGTTGTGCCC ATGTCTTCAG CCCCATCTGA AACCAGCTAC 
AGTGGCCCAG CAGTGAGCAG CGGCTATGAG CAGGGCAAGG TCCCTGAGGT GCCCCGGGCT 
GGTGACCGTG GCAGTGTGAG CCAGAGCCCA GCCCCCACCT ACCCCTCTGA CTCACACTAT 
ACCAGTCTGG AGCAGAACGT TCCTCGAAAC TACGTAATGA TTGATGACAT CAGTGAACTG 
ACCAAGGACA GCACCTCTAC TGCTCCTGAT AGCCAACGGC TGGAGCCCCT GGGGCCAGGC 
AGCAGTGGGC GTCCAGGGAA GGAGCCTGGA GAACCAGGTG TCCTTGACGG GCCCACACTG 
CCCTGCTGCT ATGCCAGAGG AGAAGAGGAA TCTGAGGAGG ACTCATACGA TCCCCGCGGG 
AAGGGTGGCC ACCTCCGGAG CATGGAGAGC AATGGTCGAC CAGCCAGTAC CCACTACTAT 
GGTGACAGTG ACTACAGGCA TGGGGCTCGA GTAGAGAAGT ATGGTCCAGG GCCCATGGGG 
CCCAAGCATC CCTCCAAGAG CCTGGCTCCA GCTGCCATCT CCTCAAAGCG CAGCAAGCAC 
CGGAAGCAGG GCATGGAGCA AAAGATATCC AAGTTCTCGC CTATTGAAGA GGCCAAAGAC 
GTAGAGTCAG ACCTGGCGTC CTACCCCCCA CCTGCAGTCA GCAGCAGCCT GGTCTCTCGG 
GGCAGGAAGT TCCAGGATGA AATCACCTAT GGGCTCAAGA AGAACGTGTA TGAGCAGCAA 
AAATACTATG GGATGTCCAG CCGGGACGCA GTGGAGGACG ACCGCATTTA TGGCGGGAGC 
AGCCGGTCCC GGGCACCTTC TGCATACAGT GGGGAGAAGC TGTCCAGCCA CGACTTCAGT 
GGCTGGGGCA AGGGGTACGA AAGGGAACGG GAGGCTGTGG AGCGACTTCA AAAAGCGGGC 
CCCAAGCCCT CATCCCTAAG TATGGCCCAC AGCCGGGTAC GACCCCCCAT GCGGAGCCAG 
GCCTCTGAAG AGGAGAGCCC CGTCAGTCCT TTGGGGAGGC CCCGCCCTGC CGGAGGGCCC 
CTCCCTCCCG GCGGGGATAC CTGCCCACAG TTCTGCTCCA GCCACTCCAT GCCTGATGTC 
CAGGAACATG TCAAGGACGG ACCTCGGGCC CACGCATATA AGCGTGAGGA GGGCTACATC 
CTGGATGATT CCCATTGCGT GGTTTCCGAC AGCGAAGCGT ATCACCTGGG CCAGGAGGAG 
ACGGACTGGT TTGATAAGCC CCGGGATGCC CGCTCTGACC GGTTCAGGCA CCACGGGGGC 
CATGCAGTTT CCTCCTCCTC CCAGAAGCGA GGCCCTGCCA GGCACAGCTA CCATGACTAC 
GATGAACCCC CTGAGGAGGG CCTGTGGCCT CATGATGAGG GTGGCCCAGG CCGCCATGCC 
TCAGCCAAGG AACACCGGCA CGGTGACCAC GGGCGGCACT CAGGCCGCCA CACTGGTGAG 
GAGCCGGGAC GGCGTGCTGC CAAACCACAC GCTCGGGACC TGGGTCGCCA TGAGGCCCGG 
CCCCACTCTC AGCCCAGCTC TGCTCCAGCT ATGCCGAAGA AGGGTCAGCC TGGGTATCCC 
AGCTCTGCTG AGTACTCACA GCCATCCCGT GCTTCATCCG CATACCATCA TGCCTCTGAC 
AGCAAGAAGG GCTCCCGGCA AGCCCACTCC GGGCCCGCTG CACTGCAGTC AAAGGCAGAA 
CCCCAGGCGC AGCCGCAGCT GCAAGGTCGG CAGGCAGCTC CAGGACCACA GCAGTCACAG 
TCACCATCAT CCAGGCAAAT ACCCTCTGGG GCAGCATCAC GCCAGCCACA GACACAGCAG 
CAGCAGCAAG GTCTTGGGCT GCAGCCCCCA CAGCAGGCTC TGACACAGGC TCGGCTGCAG 
CAACAGAGCC AGCCAACCAC CCGGGGCTCA GCCCCTGCTG CCAGCCAGCC TGCAGGGAAG 
CCTCAGCCAG GCCCCAGCAC AGCCACAGGT CCTCAACCAG CAGGACCGCC ACGGGCAGAA 
CAGACAAATG GCTCTAAAGG GACAGCCAAA GCACCGCAAC AGGGGAGGGC TCCTCAGGCC 
CAGCCAGCAC CAGGACCTGG ACCTGCAGGT GTGAAGGCTG GAGCCAGGCC TGGAGGAACC 
CCAGGGGCTC CCGCCGGCCA GCCAGGTGCC GATGGGGAGA GCGTGTTCTC CAAGATCCTC 
CCTGGCGGGG CAGCCGAGCA AGCTGGCAAA CTGACGGAAG CTGTCTCTGC TTTTGGCAAA 
AAATTTTCCT CATTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BSN ( NM_003458.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003449.2 Homo sapiens bassoon presynaptic cytomatrix protein (BSN), mRNA.

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CloneID	OHu19210
Clone ID Related Accession (Same CDS sequence)	NM_003458.3 , NM_003458.4
Accession Version	NM_003458.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	protein bassoon
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104452.2, AF052224.1, AC099668.2, AB007894.1 and AW131629.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_003458.3. CCDS Note: This CCDS ID represents the protein described in PMIDs: 10329005 and 9679147. This transcript is supported by AF052224.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMIDs: 9679147 and 19165527. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF052224.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000296452.5/ ENSP00000296452.4 NMD candidate :: PMID: 10329005 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAACG AGGTCAGCCT GGAGGGCGGC GCTGGCGACG GGCCGCTGCC GCCCGGCGGC 
GCCGGCCCCG GCCCGGGCCC CGGCCCCGGC CCCGGCGCAG GAAAGCCGCC TTCAGCACCG 
GCCGGTGGCG GACAGCTCCC CGCGGCGGGA GCAGCGCGGT CGACCGCGGT ACCACCGGTC 
CCTGGCCCCG GCCCCGGCCC CGGTCCCGGC CCTGGCCCGG GCAGCACTTC CCGGAGACTG 
GACCCCAAGG AACCCCTGGG TAACCAGAGA GCAGCTTCCC CAACTCCGAA GCAGGCTTCT 
GCTACCACTC CTGGCCATGA GAGCCCCCGA GAGACAAGGG CACAGGGACC AGCAGGCCAG 
GAGGCTGATG GTCCCCGCAG GACGCTGCAG GTAGACAGCA GGACACAGAG ATCAGGGCGG 
TCCCCCTCAG TGTCACCGGA CAGAGGCAGC ACCCCCACAT CACCCTACTC CGTCCCTCAG 
ATCGCCCCCC TTCCCAGCAG CACGCTGTGT CCCATATGCA AGACTTCGGA CCTCACGTCG 
ACCCCCAGCC AGCCAAACTT CAACACCTGC ACCCAGTGTC ACAACAAGGT CTGCAACCAG 
TGTGGGTTCA ACCCCAACCC TCATCTCACC CAGGTGAAGG AGTGGCTCTG TCTGAACTGT 
CAGATGCAGA GGGCTCTGGG AATGGACATG ACCACTGCAC CTCGCTCCAA GAGCCAGCAG 
CAGCTGCACT CCCCAGCCCT GTCTCCTGCC CACTCCCCGG CCAAACAGCC CCTGGGGAAG 
CCAGACCAAG AGAGATCTCG GGGCCCAGGA GGACCACAGC CTGGGTCCCG CCAGGCTGAG 
ACAGCCAGGG CCACCTCAGT GCCGGGGCCT GCCCAAGCAG CTGCCCCTCC AGAGGTGGGG 
AGGGTGTCTC CTCAGCCCCC TCAACCCACC AAGCCTTCCA CAGCTGAGCC CAGGCCACCT 
GCAGGAGAGG CCCCGGCCAA AAGTGCCACC GCAGTGCCCG CTGGGCTTGG TGCCACTGAG 
CAGACCCAGG AGGGCCTCAC TGGTAAGCTC TTCGGCCTTG GCGCGTCACT GCTAACCCAG 
GCGAGCACCC TCATGTCTGT GCAGCCCGAG GCTGACACCC AGGGCCAGCC TGCCCCCAGC 
AAGGGGACAC CTAAGATCGT CTTCAATGAT GCCAGCAAGG AGGCTGGCCC AAAACCCTTG 
GGCTCAGGGC CCGGGCCTGG GCCAGCACCT GGAGCCAAAA CTGAGCCTGG GGCTAGAATG 
GGTCCTGGAT CTGGACCTGG AGCCCTGCCG AAAACTGGGG GAACAACCAG TCCAAAGCAT 
GGCAGAGCAG AACATCAGGC AGCATCGAAG GCTGCTGCCA AGCCAAAGAC CATGCCGAAG 
GAAAGGGCCA TCTGCCCACT GTGCCAAGCC GAGCTCAACG TGGGCAGCAA GAGCCCAGCC 
AACTATAACA CATGCACCAC CTGCAGGCTC CAGGTGTGCA ACCTGTGTGG CTTCAACCCA 
ACACCCCACC TGGTGGAGAA AACAGAGTGG CTCTGTCTGA ACTGCCAAAC CAAGCGGCTA 
CTGGAGGGCA GCCTAGGAGA GCCGACCCCC CTGCCGCCGC CCACCTCACA GCAGCCCCCT 
GTAGGGGCCC CTCACCGTGC ATCTGGAACA TCCCCTCTGA AGCAGAAAGG GCCACAGGGG 
CTGGGCCAGC CTTCAGGCCC CCTGCCTGCC AAGGCCAGCC CTCTATCCAC CAAGGCCAGC 
CCTCTGCCCA GCAAGGCCAG CCCCCAGGCC AAGCCCCTCA GGGCTTCTGA ACCCAGCAAG 
ACCCCAAGCA GTGTCCAGGA AAAGAAGACC CGAGTCCCCA CTAAAGCTGA GCCCATGCCG 
AAGCCACCTC CAGAGACTAC CCCAACCCCT GCGACTCCTA AAGTAAAGAG TGGGGTGAGG 
AGGGCTGAAC CTGCCACCCC TGTCGTCAAG GCTGTTCCAG AAGCCCCCAA GGGTGGGGAG 
GCGGAGGACC TGGTGGGCAA GCCTTACTCT CAGGATGCGT CTCGGAGCCC ACAGAGCCTC 
AGTGACACAG GCTATTCCTC TGACGGCATC TCTAGCTCCC AGAGTGAGAT CACAGGAGTC 
GTGCAGCAGG AGGTGGAACA GCTGGACAGT GCAGGGGTGA CAGGGCCACA TCCACCCAGC 
CCCTCCGAGA TCCACAAGGT GGGGAGCAGC ATGCGGCCTT TGCTGCAGGC CCAGGGCCTG 
GCCCCAAGTG AGCGGAGCAA GCCACTCTCC AGCGGTACTG GCGAGGAGCA GAAGCAGCGG 
CCCCACTCCT TGTCCATCAC GCCTGAGGCC TTTGACTCTG ATGAGGAGCT GGAGGATATC 
CTGGAGGAAG ACGAAGACTC TGCTGAGTGG AGGCGCCGGA GAGAGCAGCA GGACACTGCC 
GAGTCCTCAG ACGACTTTGG CAGCCAATTG AGGCACGACT ATGTGGAGGA CAGCAGTGAG 
GGTGGCCTGT CCCCTCTTCC ACCCCAGCCC CCAGCCCGGG CAGCAGAACT GACTGATGAG 
GATTTCATGC GACGGCAGAT TCTCGAGATG AGCGCCGAGG AAGACAACCT GGAGGAGGAT 
GACACTGCCA CCTCCGGGCG TGGCCTGGCC AAACATGGCA CCCAGAAAGG TGGCCCCAGA 
CCCAGGCCTG AGCCTAGCCA AGAACCAGCA GCACTGCCCA AGAGGCGCCT GCCCCACAAT 
GCCACCACGG GCTATGAGGA GCTGCTCCCT GAGGGAGGCT CAGCAGAGGC TACCGATGGC 
AGTGGGACCC TGCAGGGTGG GCTCCGTCGC TTCAAGACCA TTGAGCTCAA CAGCACGGGA 
AGTTATGGTC ATGAGTTGGA CCTGGGCCAA GGCCCAGACC CCAGTCTGGA CCGGGAGCCT 
GAGCTGGAGA TGGAGAGCCT AACGGGCTCC CCTGAGGACC GCTCCCGTGG TGAGCACTCC 
TCTACATTGC CTGCCTCCAC ACCCAGCTAC ACCTCGGGCA CCTCTCCCAC CTCTCTGTCC 
TCCCTAGAGG AGGACAGTGA CAGCAGCCCC AGCCGCAGGC AGCGTCTAGA AGAAGCAAAG 
CAGCAGCGCA AGGCCCGGCA CCGCTCCCAC GGGCCCCTGC TACCCACCAT CGAGGACTCC 
TCAGAGGAGG AGGAGCTGCG GGAGGAAGAG GAGCTGCTTC GTGAGCAAGA GAAGATGCGG 
GAGGTGGAGC AGCAGCGCAT CCGCAGCACG GCCCGCAAGA CCCGGCGGGA CAAGGAAGAA 
CTGCGGGCCC AGCGGAGGCG AGAGCGCTCC AAGACACCAC CCAGTAACTT GTCACCCATC 
GAGGATGCCT CCCCGACGGA GGAGCTGAGG CAGGCGGCCG AGATGGAGGA GCTACACCGC 
TCCTCCTGCT CTGAGTACTC ACCCTCACCC TCCCTTGACT CTGAGGCTGA GGCCTTGGAT 
GGTGGCCCTA GCCGGCTTTA CAAGTCAGGC AGTGAGTACA ACCTGCCCAC CTTCATGTCC 
CTCTACTCAC CAACCGAGAC ACCCTCCGGC AGCTCCACCA CTCCCAGTTC CGGACGGCCG 
CTCAAGAGCG CTGAGGAGGC TTATGAGGAG ATGATGCGCA AAGCTGAGCT GCTCCAGAGG 
CAGCAAGGCC AGGCAGCAGG GGCCCGGGGA CCCCATGGCG GCCCCTCTCA GCCCACAGGC 
CCCCGGGGCC TGGGCTCCTT CGAATATCAA GACACTACAG ACCGTGAGTA TGGCCAGGCT 
GCTCAGCCTG CCGCAGAGGG CACGCCAGCC AGCCTGGGAG CAGCCGTGTA CGAAGAAATC 
CTTCAGACAT CACAGAGCAT AGTCCGCATG CGGCAGGCCT CCTCACGAGA CCTGGCTTTT 
GCTGAGGACA AAAAGAAGGA GAAGCAGTTT CTAAATGCTG AGAGTGCATA CATGGACCCA 
ATGAAGCAAA ATGGTGGCCC CCTTACCCCT GGTACCAGTC CCACCCAGCT CGCTGCCCCT 
GTGTCCTTCT CTACCCCCAC CTCCTCAGAC AGCAGCGGGG GCCGAGTTAT TCCCGATGTC 
CGTGTCACTC AGCATTTTGC AAAGGAGACT CAGGACCCCC TCAAGCTGCA CAGCTCTCCT 
GCCTCCCCCA GCTCAGCCTC CAAGGAGATA GGCATGCCCT TTTCCCAGGG CCCTGGGACC 
CCAGCCACCA CAGCTGTGGC TCCTTGTCCA GCTGGGCTGC CACGAGGATA TATGACTCCA 
GCCTCCCCAG CAGGCTCCGA GCGTAGTCCT TCACCATCTT CCACAGCCCA CAGCTATGGA 
CACAGCCCAA CCACTGCAAA CTATGGGTCC CAAACTGAGG ATCTACCCCA GGCCCCCAGT 
GGCCTTGCTG CAGCTGGACG AGCTGCTAGA GAGAAGCCCT TGAGTGCGAG TGACGGTGAG 
GGTGGCACTC CTCAGCCTTC CCGGGCATAT TCCTACTTTG CAAGCTCCAG CCCACCTCTC 
TCCCCGTCTT CCCCCTCAGA GAGTCCCACA TTCTCCCCTG GCAAGATGGG CCCAAGGGCC 
ACAGCAGAGT TCTCTACACA GACGCCAAGT CCAGCCCCTG CCTCAGACAT GCCACGGAGC 
CCTGGTGCCC CCACTCCATC ACCTATGGTA GCCCAGGGTA CACAAACACC ACATCGACCC 
AGCACGCCTC GCCTGGTGTG GCAGGAGTCC TCTCAAGAGG CTCCCTTTAT GGTCATCACG 
CTGGCATCTG ACGCCTCCAG CCAGACCAGG ATGGTACATG CCAGTGCCTC CACCTCCCCG 
CTCTGCTCAC CTACTGAAAC CCAGCCCACC ACCCATGGCT ACAGCCAGAC AACACCTCCG 
AGTGTGTCTC AGCTGCCCCC AGAGCCACCT GGGCCACCTG GCTTTCCACG GGTGCCCAGT 
GCTGGTGCAG ATGGGCCCCT GGCACTATAT GGCTGGGGTG CCCTCCCTGC TGAGAACATC 
TCCCTGTGCC GGATCTCCTC TGTCCCTGGG ACGTCTAGGG TTGAGCCAGG CCCCAGGACC 
CCTGGCACTG CAGTGGTAGA CCTCCGTACA GCTGTCAAGC CCACTCCCAT CATCCTCACT 
GACCAGGGCA TGGACCTCAC CTCTCTTGCT GTGGAAGCGA GGAAGTATGG TCTTGCCCTG 
GATCCAATCC CAGGACGGCA GTCGACCGCC GTGCAGCCCT TGGTTATCAA CCTCAATGCC 
CAGGAGCATA CCTTCCTTGC TACTGCCACC ACCGTGAGCA TCACCATGGC CTCGTCTGTG 
TTCATGGCTC AACAAAAGCA GCCTGTGGTC TATGGAGACC CCTACCAGAG CCGCCTTGAC 
TTTGGCCAGG GTGGGGGTAG CCCTGTGTGC CTGGCCCAGG TCAAACAAGT AGAGCAGGCT 
GTCCAGACAG CCCCATACCG AAGTGGGCCC CGGGGAAGAC CCAGGGAGGC CAAGTTTGCC 
AGATATAACC TACCCAACCA AGTAGCTCCT CTGGCCAGAA GAGACGTTTT GATCACTCAG 
ATGGGCACCG CCCAGAGCAT TGGCCTCAAG CCAGGCCCAG TGCCAGAGCC AGGTGCCGAG 
CCCCACCGGG CCACCCCTGC AGAGCTGCGG TCACATGCTC TGCCAGGTGC CAGGAAGCCA 
CACACAGTGG TGGTGCAGAT GGGAGAGGGC ACAGCAGGCA CTGTGACCAC ACTGCTCCCA 
GAGGAGCCTG CGGGTGCCCT GGACCTTACC GGGATGAGGC CTGAGAGCCA GCTGGCATGC 
TGTGACATGG TCTACAAGCT CCCCTTTGGC AGCAGCTGCA CTGGCACCTT CCACCCGGCC 
CCCAGTGTGC CTGAGAAGAG CATGGCAGAT GCTGCCCCAC CTGGCCAAAG CAGCAGCCCC 
TTCTATGGTC CCCGGGACCC TGAGCCTCCT GAGCCCCCAA CCTACCGGGC ACAGGGGGTG 
GTGGGGCCTG GGCCCCATGA GGAGCAGAGG CCCTACCCAC AAGGCCTGCC TGGTAGGCTG 
TACTCCTCCA TGTCTGACAC CAATTTGGCT GAGGCTGGCC TCAACTACCA TGCCCAGAGG 
ATCGGGCAGC TCTTCCAGGG TCCTGGACGA GACTCGGCTA TGGACCTCAG CTCACTGAAG 
CACTCCTACA GCCTGGGCTT TGCGGATGGA CGCTACCTAG GGCAGGGCTT GCAGTATGGC 
TCAGTCACGG ACCTGCGTCA TCCTACAGAC CTTTTGGCTC ACCCGCTTCC CATGCGGCGC 
TATAGCTCAG TGTCGAACAT CTACTCAGAC CACAGGTACG GCCCACGGGG AGATGCAGTT 
GGCTTCCAGG AGGCCAGCCT GGCCCAGTAC AGTGCCACCA CAGCCCGTGA AATCAGTCGC 
ATGTGCGCTG CCCTCAACTC CATGGACCAG TATGGTGGGC GGCATGGCAG TGGTGGTGGT 
GGCCCTGACC TTGTGCAGTA CCAGCCCCAG CACGGGCCCG GGCTCAGTGC TCCACAGAGT 
CTGGTTCCCC TCAGACCTGG ACTCCTTGGT AACCCCACCT TTCCAGAGGG CCACCCAAGT 
CCTGGGAACT TGGCCCAGTA TGGGCCTGCA GCAGGCCAAG GAACAGCAGT CAGACAGCTG 
CTGCCGTCCA CAGCCACTGT ACGTGCAGCT GATGGCATGA TCTACTCGAC TATCAATACC 
CCAATTGCTG CAACACTGCC CATCACCACC CAGCCTGCCT CAGTCCTGCG GCCCATGGTG 
CGTGGTGGCA TGTACAGGCC TTACGCATCT GGTGGAATCA CAGCCGTGCC ACTCACCAGT 
CTGACACGTG TGCCCATGAT TGCCCCCCGG GTACCTCTTG GACCCACAGG GCTGTACCGC 
TATCCTGCAC CAAGTAGATT TCCCATTGCT TCCAGTGTTC CACCTGCAGA AGGGCCTGTC 
TATCTGGGGA AACCTGCTGC TGCCAAGGCC CCTGGGGCTG GGGGCCCTTC AAGGCCAGAG 
ATGCCAGTAG GGGCTGCACG GGAAGAGCCT CTTCCCACAA CCACCCCTGC TGCCATCAAG 
GAGGCTGCAG GAGCCCCAGC TCCTGCCCCA CTAGCTGGCC AGAAGCCACC AGCAGATGCT 
GCTCCTGGGG GTGGCAGTGG GGCCCTCAGC CGGCCAGGGT TCGAGAAAGA GGAAGCATCA 
CAGGAGGAGA GGCAGCGGAA GCAACAGGAG CAGCTGCTCC AGCTAGAGCG GGAGCGGGTG 
GAGTTGGAGA AGCTGCGACA ACTTCGGCTG CAAGAGGAGC TAGAGCGGGA ACGTGTGGAG 
CTGCAGAGGC ACCGTGAGGA GGAGCAGCTG CTGGTGCAGC GGGAGTTGCA GGAGCTGCAG 
ACCATCAAGC ACCATGTGCT GCAGCAGCAG CAAGAGGAAC GCCAGGCTCA ATTTGCACTG 
CAGCGGGAAC AGCTAGCGCA GCAGCGTCTG CAGCTGGAGC AGATCCAGCA GCTGCAGCAG 
CAGCTGCAGC AGCAGCTAGA GGAGCAGAAG CAGCGGCAGA AGGCTCCCTT TCCTGCAGCC 
TGTGAGGCAC CTGGCCGAGG GCCTCCCCTA GCGGCTGCTG AGTTGGCCCA GAATGGCCAG 
TATTGGCCCC CCCTTACACA TGCAGCCTTC ATTGCCATGG CAGGGCCTGA AGGACTTGGG 
CAGCCTCGTG AGCCTGTGCT GCACCGGGGT CTCCCCAGCT CTGCCTCAGA CATGTCACTG 
CAAACGGAGG AGCAGTGGGA GGCCAGCCGT AGTGGCATCA AGAAGCGGCA CTCCATGCCA 
CGCCTGCGGG ATGCCTGTGA GCTAGAGTCT GGGACTGAGC CCTGTGTGGT CAGGAGGATT 
GCCGACAGCA GCGTGCAGAC AGACGATGAG GATGGGGAGA GCCGCTACCT CTTGAGTCGG 
CGACGCCGGG CACGGCGGAG TGCTGACTGC AGTGTGCAGA CGGACGACGA AGACAGTGCT 
GAGTGGGAGC AGCCAGTGCG CCGCCGCAGG TCTCGTCTTC CCCGCCACTC AGACTCAGGC 
TCTGACAGCA AGCACGATGC CACTGCCTCA TCATCCAGTG CTGCTGCCAC TGTGAGGGCC 
ATGAGCAGCG TGGGCATCCA GACCATCAGT GACTGCTCCG TGCAGACGGA GCCTGACCAG 
CTGCCCAGGG TCTCTCCAGC CATCCACATC ACAGCTGCCA CCGATCCCAA GGTGGAGATC 
GTCAGGTACA TATCGGCGCC AGAGAAGACT GGGCGTGGGG AGAGCCTGGC CTGCCAGACG 
GAGCCAGATG GGCAGGCCCA GGGTGTAGCC GGGCCGCAGC TTGTAGGGCC AACTGCCATC 
AGCCCCTACC TGCCTGGCAT CCAGATCGTC ACCCCAGGGC CTCTGGGCAG ATTTGAAAAA 
AAGAAGCCAG ATCCCCTGGA GATTGGGTAC CAGGCCCACC TGCCTCCGGA GTCTCTCTCA 
CAGCTTGTGA GCCGCCAGCC TCCCAAGTCC CCTCAGGTCC TCTACTCACC AGTCTCACCC 
CTGTCCCCTC ACCGGCTCCT GGACACCTCC TTTGCTTCCA GTGAGAGGCT GAACAAAGCT 
CACGTGAGTC CCCAGAAGCA CTTCACGGCT GACAGCGCTC TCCGCCAGCA GACGCTGCCT 
CGCCCCATGA AGACCCTGCA GCGGTCCCTG TCTGACCCTA AGCCCCTCAG CCCCACCGCC 
GAAGAGTCTG CCAAAGAGAG ATTCTCCCTC TACCAGCACC AGGGGGGACT GGGTAGCCAG 
GTGTCGGCGT TGCCACCCAA CAGCCTGGTC CGCAAGGTGA AGCGGACACT GCCCAGCCCC 
CCTCCAGAGG AGGCTCACCT TCCCCTGGCT GGCCAGGCCT CCCCACAGCT GTATGCAGCC 
AGCCTGCTGC AGCGAGGGCT GACGGGGCCC ACCACTGTCC CTGCTACCAA GGCCAGCCTG 
CTCCGGGAGC TGGACCGGGA CCTGCGGCTG GTGGAGCATG AGTCCACCAA ACTGCGCAAG 
AAGCAGGCAG AGCTGGATGA GGAGGAGAAG GAGATTGACG CCAAGCTCAA GTACCTGGAG 
TTGGGTATCA CACAACGCAA AGAGTCTTTG GCCAAAGACC GGGGTGGCCG TGACTACCCA 
CCCTTGCGTG GTCTTGGCGA GCATCGTGAC TACCTATCGG ACAGTGAGCT CAACCAGCTG 
CGGCTCCAGG GCTGCACCAC TCCCGCTGGC CAGTTTGTGG ACTTCCCTGC CACTGCCGCT 
GCTCCTGCCA CCCCCTCTGG TCCCACTGCC TTCCAGCAGC CCCGCTTCCA GCCTCCAGCC 
CCACAGTATT CTGCAGGCAG TGGTGGGCCA ACTCAGAACG GATTCCCAGC CCACCAGGCC 
CCCACCTACC CTGGCCCCAG CACGTACCCA GCTCCTGCCT TTCCTCCTGG TGCCAGTTAC 
CCAGCTGAGC CTGGCCTGCC AAACCAGCAG GCTTTCCGCC CCACAGGCCA CTATGCAGGC 
CAAACACCCA TGCCAACCAC ACAGAGCACC CTTTTTCCAG TCCCCGCTGA TAGCCGTGCC 
CCACTGCAGA AGCCACGCCA GACATCGCTA GCCGACTTGG AGCAGAAGGT GCCCACCAAC 
TATGAGGTGA TCGCCAGCCC CGTTGTGCCC ATGTCTTCAG CCCCATCTGA AACCAGCTAC 
AGTGGCCCAG CAGTGAGCAG CGGCTATGAG CAGGGCAAGG TCCCTGAGGT GCCCCGGGCT 
GGTGACCGTG GCAGTGTGAG CCAGAGCCCA GCCCCCACCT ACCCCTCTGA CTCACACTAT 
ACCAGTCTGG AGCAGAACGT TCCTCGAAAC TACGTAATGA TTGATGACAT CAGTGAACTG 
ACCAAGGACA GCACCTCTAC TGCTCCTGAT AGCCAACGGC TGGAGCCCCT GGGGCCAGGC 
AGCAGTGGGC GTCCAGGGAA GGAGCCTGGA GAACCAGGTG TCCTTGACGG GCCCACACTG 
CCCTGCTGCT ATGCCAGAGG AGAAGAGGAA TCTGAGGAGG ACTCATACGA TCCCCGCGGG 
AAGGGTGGCC ACCTCCGGAG CATGGAGAGC AATGGTCGAC CAGCCAGTAC CCACTACTAT 
GGTGACAGTG ACTACAGGCA TGGGGCTCGA GTAGAGAAGT ATGGTCCAGG GCCCATGGGG 
CCCAAGCATC CCTCCAAGAG CCTGGCTCCA GCTGCCATCT CCTCAAAGCG CAGCAAGCAC 
CGGAAGCAGG GCATGGAGCA AAAGATATCC AAGTTCTCGC CTATTGAAGA GGCCAAAGAC 
GTAGAGTCAG ACCTGGCGTC CTACCCCCCA CCTGCAGTCA GCAGCAGCCT GGTCTCTCGG 
GGCAGGAAGT TCCAGGATGA AATCACCTAT GGGCTCAAGA AGAACGTGTA TGAGCAGCAA 
AAATACTATG GGATGTCCAG CCGGGACGCA GTGGAGGACG ACCGCATTTA TGGCGGGAGC 
AGCCGGTCCC GGGCACCTTC TGCATACAGT GGGGAGAAGC TGTCCAGCCA CGACTTCAGT 
GGCTGGGGCA AGGGGTACGA AAGGGAACGG GAGGCTGTGG AGCGACTTCA AAAAGCGGGC 
CCCAAGCCCT CATCCCTAAG TATGGCCCAC AGCCGGGTAC GACCCCCCAT GCGGAGCCAG 
GCCTCTGAAG AGGAGAGCCC CGTCAGTCCT TTGGGGAGGC CCCGCCCTGC CGGAGGGCCC 
CTCCCTCCCG GCGGGGATAC CTGCCCACAG TTCTGCTCCA GCCACTCCAT GCCTGATGTC 
CAGGAACATG TCAAGGACGG ACCTCGGGCC CACGCATATA AGCGTGAGGA GGGCTACATC 
CTGGATGATT CCCATTGCGT GGTTTCCGAC AGCGAAGCGT ATCACCTGGG CCAGGAGGAG 
ACGGACTGGT TTGATAAGCC CCGGGATGCC CGCTCTGACC GGTTCAGGCA CCACGGGGGC 
CATGCAGTTT CCTCCTCCTC CCAGAAGCGA GGCCCTGCCA GGCACAGCTA CCATGACTAC 
GATGAACCCC CTGAGGAGGG CCTGTGGCCT CATGATGAGG GTGGCCCAGG CCGCCATGCC 
TCAGCCAAGG AACACCGGCA CGGTGACCAC GGGCGGCACT CAGGCCGCCA CACTGGTGAG 
GAGCCGGGAC GGCGTGCTGC CAAACCACAC GCTCGGGACC TGGGTCGCCA TGAGGCCCGG 
CCCCACTCTC AGCCCAGCTC TGCTCCAGCT ATGCCGAAGA AGGGTCAGCC TGGGTATCCC 
AGCTCTGCTG AGTACTCACA GCCATCCCGT GCTTCATCCG CATACCATCA TGCCTCTGAC 
AGCAAGAAGG GCTCCCGGCA AGCCCACTCC GGGCCCGCTG CACTGCAGTC AAAGGCAGAA 
CCCCAGGCGC AGCCGCAGCT GCAAGGTCGG CAGGCAGCTC CAGGACCACA GCAGTCACAG 
TCACCATCAT CCAGGCAAAT ACCCTCTGGG GCAGCATCAC GCCAGCCACA GACACAGCAG 
CAGCAGCAAG GTCTTGGGCT GCAGCCCCCA CAGCAGGCTC TGACACAGGC TCGGCTGCAG 
CAACAGAGCC AGCCAACCAC CCGGGGCTCA GCCCCTGCTG CCAGCCAGCC TGCAGGGAAG 
CCTCAGCCAG GCCCCAGCAC AGCCACAGGT CCTCAACCAG CAGGACCGCC ACGGGCAGAA 
CAGACAAATG GCTCTAAAGG GACAGCCAAA GCACCGCAAC AGGGGAGGGC TCCTCAGGCC 
CAGCCAGCAC CAGGACCTGG ACCTGCAGGT GTGAAGGCTG GAGCCAGGCC TGGAGGAACC 
CCAGGGGCTC CCGCCGGCCA GCCAGGTGCC GATGGGGAGA GCGTGTTCTC CAAGATCCTC 
CCTGGCGGGG CAGCCGAGCA AGCTGGCAAA CTGACGGAAG CTGTCTCTGC TTTTGGCAAA 
AAATTTTCCT CATTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003449.2
CDS	127..11907
Translation	MGNEVSLEGGAGDGPLPPGGAGPGPGPGPGPGAGKPPSAPAGGGQLPAAGAARSTAVPPVPGPGPGPGPGPGPGSTSRRLDPKEPLGNQRAASPTPKQASATTPGHESPRETRAQGPAGQEADGPRRTLQVDSRTQRSGRSPSVSPDRGSTPTSPYSVPQIAPLPSSTLCPICKTSDLTSTPSQPNFNTCTQCHNKVCNQCGFNPNPHLTQVKEWLCLNCQMQRALGMDMTTAPRSKSQQQLHSPALSPAHSPAKQPLGKPDQERSRGPGGPQPGSRQAETARATSVPGPAQAAAPPEVGRVSPQPPQPTKPSTAEPRPPAGEAPAKSATAVPAGLGATEQTQEGLTGKLFGLGASLLTQASTLMSVQPEADTQGQPAPSKGTPKIVFNDASKEAGPKPLGSGPGPGPAPGAKTEPGARMGPGSGPGALPKTGGTTSPKHGRAEHQAASKAAAKPKTMPKERAICPLCQAELNVGSKSPANYNTCTTCRLQVCNLCGFNPTPHLVEKTEWLCLNCQTKRLLEGSLGEPTPLPPPTSQQPPVGAPHRASGTSPLKQKGPQGLGQPSGPLPAKASPLSTKASPLPSKASPQAKPLRASEPSKTPSSVQEKKTRVPTKAEPMPKPPPETTPTPATPKVKSGVRRAEPATPVVKAVPEAPKGGEAEDLVGKPYSQDASRSPQSLSDTGYSSDGISSSQSEITGVVQQEVEQLDSAGVTGPHPPSPSEIHKVGSSMRPLLQAQGLAPSERSKPLSSGTGEEQKQRPHSLSITPEAFDSDEELEDILEEDEDSAEWRRRREQQDTAESSDDFGSQLRHDYVEDSSEGGLSPLPPQPPARAAELTDEDFMRRQILEMSAEEDNLEEDDTATSGRGLAKHGTQKGGPRPRPEPSQEPAALPKRRLPHNATTGYEELLPEGGSAEATDGSGTLQGGLRRFKTIELNSTGSYGHELDLGQGPDPSLDREPELEMESLTGSPEDRSRGEHSSTLPASTPSYTSGTSPTSLSSLEEDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLREQEKMREVEQQRIRSTARKTRRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSPSLDSEAEALDGGPSRLYKSGSEYNLPTFMSLYSPTETPSGSSTTPSSGRPLKSAEEAYEEMMRKAELLQRQQGQAAGARGPHGGPSQPTGPRGLGSFEYQDTTDREYGQAAQPAAEGTPASLGAAVYEEILQTSQSIVRMRQASSRDLAFAEDKKKEKQFLNAESAYMDPMKQNGGPLTPGTSPTQLAAPVSFSTPTSSDSSGGRVIPDVRVTQHFAKETQDPLKLHSSPASPSSASKEIGMPFSQGPGTPATTAVAPCPAGLPRGYMTPASPAGSERSPSPSSTAHSYGHSPTTANYGSQTEDLPQAPSGLAAAGRAAREKPLSASDGEGGTPQPSRAYSYFASSSPPLSPSSPSESPTFSPGKMGPRATAEFSTQTPSPAPASDMPRSPGAPTPSPMVAQGTQTPHRPSTPRLVWQESSQEAPFMVITLASDASSQTRMVHASASTSPLCSPTETQPTTHGYSQTTPPSVSQLPPEPPGPPGFPRVPSAGADGPLALYGWGALPAENISLCRISSVPGTSRVEPGPRTPGTAVVDLRTAVKPTPIILTDQGMDLTSLAVEARKYGLALDPIPGRQSTAVQPLVINLNAQEHTFLATATTVSITMASSVFMAQQKQPVVYGDPYQSRLDFGQGGGSPVCLAQVKQVEQAVQTAPYRSGPRGRPREAKFARYNLPNQVAPLARRDVLITQMGTAQSIGLKPGPVPEPGAEPHRATPAELRSHALPGARKPHTVVVQMGEGTAGTVTTLLPEEPAGALDLTGMRPESQLACCDMVYKLPFGSSCTGTFHPAPSVPEKSMADAAPPGQSSSPFYGPRDPEPPEPPTYRAQGVVGPGPHEEQRPYPQGLPGRLYSSMSDTNLAEAGLNYHAQRIGQLFQGPGRDSAMDLSSLKHSYSLGFADGRYLGQGLQYGSVTDLRHPTDLLAHPLPMRRYSSVSNIYSDHRYGPRGDAVGFQEASLAQYSATTAREISRMCAALNSMDQYGGRHGSGGGGPDLVQYQPQHGPGLSAPQSLVPLRPGLLGNPTFPEGHPSPGNLAQYGPAAGQGTAVRQLLPSTATVRAADGMIYSTINTPIAATLPITTQPASVLRPMVRGGMYRPYASGGITAVPLTSLTRVPMIAPRVPLGPTGLYRYPAPSRFPIASSVPPAEGPVYLGKPAAAKAPGAGGPSRPEMPVGAAREEPLPTTTPAAIKEAAGAPAPAPLAGQKPPADAAPGGGSGALSRPGFEKEEASQEERQRKQQEQLLQLERERVELEKLRQLRLQEELERERVELQRHREEEQLLVQRELQELQTIKHHVLQQQQEERQAQFALQREQLAQQRLQLEQIQQLQQQLQQQLEEQKQRQKAPFPAACEAPGRGPPLAAAELAQNGQYWPPLTHAAFIAMAGPEGLGQPREPVLHRGLPSSASDMSLQTEEQWEASRSGIKKRHSMPRLRDACELESGTEPCVVRRIADSSVQTDDEDGESRYLLSRRRRARRSADCSVQTDDEDSAEWEQPVRRRRSRLPRHSDSGSDSKHDATASSSSAAATVRAMSSVGIQTISDCSVQTEPDQLPRVSPAIHITAATDPKVEIVRYISAPEKTGRGESLACQTEPDGQAQGVAGPQLVGPTAISPYLPGIQIVTPGPLGRFEKKKPDPLEIGYQAHLPPESLSQLVSRQPPKSPQVLYSPVSPLSPHRLLDTSFASSERLNKAHVSPQKHFTADSALRQQTLPRPMKTLQRSLSDPKPLSPTAEESAKERFSLYQHQGGLGSQVSALPPNSLVRKVKRTLPSPPPEEAHLPLAGQASPQLYAASLLQRGLTGPTTVPATKASLLRELDRDLRLVEHESTKLRKKQAELDEEEKEIDAKLKYLELGITQRKESLAKDRGGRDYPPLRGLGEHRDYLSDSELNQLRLQGCTTPAGQFVDFPATAAAPATPSGPTAFQQPRFQPPAPQYSAGSGGPTQNGFPAHQAPTYPGPSTYPAPAFPPGASYPAEPGLPNQQAFRPTGHYAGQTPMPTTQSTLFPVPADSRAPLQKPRQTSLADLEQKVPTNYEVIASPVVPMSSAPSETSYSGPAVSSGYEQGKVPEVPRAGDRGSVSQSPAPTYPSDSHYTSLEQNVPRNYVMIDDISELTKDSTSTAPDSQRLEPLGPGSSGRPGKEPGEPGVLDGPTLPCCYARGEEESEEDSYDPRGKGGHLRSMESNGRPASTHYYGDSDYRHGARVEKYGPGPMGPKHPSKSLAPAAISSKRSKHRKQGMEQKISKFSPIEEAKDVESDLASYPPPAVSSSLVSRGRKFQDEITYGLKKNVYEQQKYYGMSSRDAVEDDRIYGGSSRSRAPSAYSGEKLSSHDFSGWGKGYEREREAVERLQKAGPKPSSLSMAHSRVRPPMRSQASEEESPVSPLGRPRPAGGPLPPGGDTCPQFCSSHSMPDVQEHVKDGPRAHAYKREEGYILDDSHCVVSDSEAYHLGQEETDWFDKPRDARSDRFRHHGGHAVSSSSQKRGPARHSYHDYDEPPEEGLWPHDEGGPGRHASAKEHRHGDHGRHSGRHTGEEPGRRAAKPHARDLGRHEARPHSQPSSAPAMPKKGQPGYPSSAEYSQPSRASSAYHHASDSKKGSRQAHSGPAALQSKAEPQAQPQLQGRQAAPGPQQSQSPSSRQIPSGAASRQPQTQQQQQGLGLQPPQQALTQARLQQQSQPTTRGSAPAASQPAGKPQPGPSTATGPQPAGPPRAEQTNGSKGTAKAPQQGRAPQAQPAPGPGPAGVKAGARPGGTPGAPAGQPGADGESVFSKILPGGAAEQAGKLTEAVSAFGKKFSSFW

Target ORF information:

RefSeq Version	NM_003458.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bassoon presynaptic cytomatrix protein (BSN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003458.4

ORF Insert Sequence:

ATGGGCAACG AGGTCAGCCT GGAGGGCGGC GCTGGCGACG GGCCGCTGCC GCCCGGCGGC 
GCCGGCCCCG GCCCGGGCCC CGGCCCCGGC CCCGGCGCAG GAAAGCCGCC TTCAGCACCG 
GCCGGTGGCG GACAGCTCCC CGCGGCGGGA GCAGCGCGGT CGACCGCGGT ACCACCGGTC 
CCTGGCCCCG GCCCCGGCCC CGGTCCCGGC CCTGGCCCGG GCAGCACTTC CCGGAGACTG 
GACCCCAAGG AACCCCTGGG TAACCAGAGA GCAGCTTCCC CAACTCCGAA GCAGGCTTCT 
GCTACCACTC CTGGCCATGA GAGCCCCCGA GAGACAAGGG CACAGGGACC AGCAGGCCAG 
GAGGCTGATG GTCCCCGCAG GACGCTGCAG GTAGACAGCA GGACACAGAG ATCAGGGCGG 
TCCCCCTCAG TGTCACCGGA CAGAGGCAGC ACCCCCACAT CACCCTACTC CGTCCCTCAG 
ATCGCCCCCC TTCCCAGCAG CACGCTGTGT CCCATATGCA AGACTTCGGA CCTCACGTCG 
ACCCCCAGCC AGCCAAACTT CAACACCTGC ACCCAGTGTC ACAACAAGGT CTGCAACCAG 
TGTGGGTTCA ACCCCAACCC TCATCTCACC CAGGTGAAGG AGTGGCTCTG TCTGAACTGT 
CAGATGCAGA GGGCTCTGGG AATGGACATG ACCACTGCAC CTCGCTCCAA GAGCCAGCAG 
CAGCTGCACT CCCCAGCCCT GTCTCCTGCC CACTCCCCGG CCAAACAGCC CCTGGGGAAG 
CCAGACCAAG AGAGATCTCG GGGCCCAGGA GGACCACAGC CTGGGTCCCG CCAGGCTGAG 
ACAGCCAGGG CCACCTCAGT GCCGGGGCCT GCCCAAGCAG CTGCCCCTCC AGAGGTGGGG 
AGGGTGTCTC CTCAGCCCCC TCAACCCACC AAGCCTTCCA CAGCTGAGCC CAGGCCACCT 
GCAGGAGAGG CCCCGGCCAA AAGTGCCACC GCAGTGCCCG CTGGGCTTGG TGCCACTGAG 
CAGACCCAGG AGGGCCTCAC TGGTAAGCTC TTCGGCCTTG GCGCGTCACT GCTAACCCAG 
GCGAGCACCC TCATGTCTGT GCAGCCCGAG GCTGACACCC AGGGCCAGCC TGCCCCCAGC 
AAGGGGACAC CTAAGATCGT CTTCAATGAT GCCAGCAAGG AGGCTGGCCC AAAACCCTTG 
GGCTCAGGGC CCGGGCCTGG GCCAGCACCT GGAGCCAAAA CTGAGCCTGG GGCTAGAATG 
GGTCCTGGAT CTGGACCTGG AGCCCTGCCG AAAACTGGGG GAACAACCAG TCCAAAGCAT 
GGCAGAGCAG AACATCAGGC AGCATCGAAG GCTGCTGCCA AGCCAAAGAC CATGCCGAAG 
GAAAGGGCCA TCTGCCCACT GTGCCAAGCC GAGCTCAACG TGGGCAGCAA GAGCCCAGCC 
AACTATAACA CATGCACCAC CTGCAGGCTC CAGGTGTGCA ACCTGTGTGG CTTCAACCCA 
ACACCCCACC TGGTGGAGAA AACAGAGTGG CTCTGTCTGA ACTGCCAAAC CAAGCGGCTA 
CTGGAGGGCA GCCTAGGAGA GCCGACCCCC CTGCCGCCGC CCACCTCACA GCAGCCCCCT 
GTAGGGGCCC CTCACCGTGC ATCTGGAACA TCCCCTCTGA AGCAGAAAGG GCCACAGGGG 
CTGGGCCAGC CTTCAGGCCC CCTGCCTGCC AAGGCCAGCC CTCTATCCAC CAAGGCCAGC 
CCTCTGCCCA GCAAGGCCAG CCCCCAGGCC AAGCCCCTCA GGGCTTCTGA ACCCAGCAAG 
ACCCCAAGCA GTGTCCAGGA AAAGAAGACC CGAGTCCCCA CTAAAGCTGA GCCCATGCCG 
AAGCCACCTC CAGAGACTAC CCCAACCCCT GCGACTCCTA AAGTAAAGAG TGGGGTGAGG 
AGGGCTGAAC CTGCCACCCC TGTCGTCAAG GCTGTTCCAG AAGCCCCCAA GGGTGGGGAG 
GCGGAGGACC TGGTGGGCAA GCCTTACTCT CAGGATGCGT CTCGGAGCCC ACAGAGCCTC 
AGTGACACAG GCTATTCCTC TGACGGCATC TCTAGCTCCC AGAGTGAGAT CACAGGAGTC 
GTGCAGCAGG AGGTGGAACA GCTGGACAGT GCAGGGGTGA CAGGGCCACA TCCACCCAGC 
CCCTCCGAGA TCCACAAGGT GGGGAGCAGC ATGCGGCCTT TGCTGCAGGC CCAGGGCCTG 
GCCCCAAGTG AGCGGAGCAA GCCACTCTCC AGCGGTACTG GCGAGGAGCA GAAGCAGCGG 
CCCCACTCCT TGTCCATCAC GCCTGAGGCC TTTGACTCTG ATGAGGAGCT GGAGGATATC 
CTGGAGGAAG ACGAAGACTC TGCTGAGTGG AGGCGCCGGA GAGAGCAGCA GGACACTGCC 
GAGTCCTCAG ACGACTTTGG CAGCCAATTG AGGCACGACT ATGTGGAGGA CAGCAGTGAG 
GGTGGCCTGT CCCCTCTTCC ACCCCAGCCC CCAGCCCGGG CAGCAGAACT GACTGATGAG 
GATTTCATGC GACGGCAGAT TCTCGAGATG AGCGCCGAGG AAGACAACCT GGAGGAGGAT 
GACACTGCCA CCTCCGGGCG TGGCCTGGCC AAACATGGCA CCCAGAAAGG TGGCCCCAGA 
CCCAGGCCTG AGCCTAGCCA AGAACCAGCA GCACTGCCCA AGAGGCGCCT GCCCCACAAT 
GCCACCACGG GCTATGAGGA GCTGCTCCCT GAGGGAGGCT CAGCAGAGGC TACCGATGGC 
AGTGGGACCC TGCAGGGTGG GCTCCGTCGC TTCAAGACCA TTGAGCTCAA CAGCACGGGA 
AGTTATGGTC ATGAGTTGGA CCTGGGCCAA GGCCCAGACC CCAGTCTGGA CCGGGAGCCT 
GAGCTGGAGA TGGAGAGCCT AACGGGCTCC CCTGAGGACC GCTCCCGTGG TGAGCACTCC 
TCTACATTGC CTGCCTCCAC ACCCAGCTAC ACCTCGGGCA CCTCTCCCAC CTCTCTGTCC 
TCCCTAGAGG AGGACAGTGA CAGCAGCCCC AGCCGCAGGC AGCGTCTAGA AGAAGCAAAG 
CAGCAGCGCA AGGCCCGGCA CCGCTCCCAC GGGCCCCTGC TACCCACCAT CGAGGACTCC 
TCAGAGGAGG AGGAGCTGCG GGAGGAAGAG GAGCTGCTTC GTGAGCAAGA GAAGATGCGG 
GAGGTGGAGC AGCAGCGCAT CCGCAGCACG GCCCGCAAGA CCCGGCGGGA CAAGGAAGAA 
CTGCGGGCCC AGCGGAGGCG AGAGCGCTCC AAGACACCAC CCAGTAACTT GTCACCCATC 
GAGGATGCCT CCCCGACGGA GGAGCTGAGG CAGGCGGCCG AGATGGAGGA GCTACACCGC 
TCCTCCTGCT CTGAGTACTC ACCCTCACCC TCCCTTGACT CTGAGGCTGA GGCCTTGGAT 
GGTGGCCCTA GCCGGCTTTA CAAGTCAGGC AGTGAGTACA ACCTGCCCAC CTTCATGTCC 
CTCTACTCAC CAACCGAGAC ACCCTCCGGC AGCTCCACCA CTCCCAGTTC CGGACGGCCG 
CTCAAGAGCG CTGAGGAGGC TTATGAGGAG ATGATGCGCA AAGCTGAGCT GCTCCAGAGG 
CAGCAAGGCC AGGCAGCAGG GGCCCGGGGA CCCCATGGCG GCCCCTCTCA GCCCACAGGC 
CCCCGGGGCC TGGGCTCCTT CGAATATCAA GACACTACAG ACCGTGAGTA TGGCCAGGCT 
GCTCAGCCTG CCGCAGAGGG CACGCCAGCC AGCCTGGGAG CAGCCGTGTA CGAAGAAATC 
CTTCAGACAT CACAGAGCAT AGTCCGCATG CGGCAGGCCT CCTCACGAGA CCTGGCTTTT 
GCTGAGGACA AAAAGAAGGA GAAGCAGTTT CTAAATGCTG AGAGTGCATA CATGGACCCA 
ATGAAGCAAA ATGGTGGCCC CCTTACCCCT GGTACCAGTC CCACCCAGCT CGCTGCCCCT 
GTGTCCTTCT CTACCCCCAC CTCCTCAGAC AGCAGCGGGG GCCGAGTTAT TCCCGATGTC 
CGTGTCACTC AGCATTTTGC AAAGGAGACT CAGGACCCCC TCAAGCTGCA CAGCTCTCCT 
GCCTCCCCCA GCTCAGCCTC CAAGGAGATA GGCATGCCCT TTTCCCAGGG CCCTGGGACC 
CCAGCCACCA CAGCTGTGGC TCCTTGTCCA GCTGGGCTGC CACGAGGATA TATGACTCCA 
GCCTCCCCAG CAGGCTCCGA GCGTAGTCCT TCACCATCTT CCACAGCCCA CAGCTATGGA 
CACAGCCCAA CCACTGCAAA CTATGGGTCC CAAACTGAGG ATCTACCCCA GGCCCCCAGT 
GGCCTTGCTG CAGCTGGACG AGCTGCTAGA GAGAAGCCCT TGAGTGCGAG TGACGGTGAG 
GGTGGCACTC CTCAGCCTTC CCGGGCATAT TCCTACTTTG CAAGCTCCAG CCCACCTCTC 
TCCCCGTCTT CCCCCTCAGA GAGTCCCACA TTCTCCCCTG GCAAGATGGG CCCAAGGGCC 
ACAGCAGAGT TCTCTACACA GACGCCAAGT CCAGCCCCTG CCTCAGACAT GCCACGGAGC 
CCTGGTGCCC CCACTCCATC ACCTATGGTA GCCCAGGGTA CACAAACACC ACATCGACCC 
AGCACGCCTC GCCTGGTGTG GCAGGAGTCC TCTCAAGAGG CTCCCTTTAT GGTCATCACG 
CTGGCATCTG ACGCCTCCAG CCAGACCAGG ATGGTACATG CCAGTGCCTC CACCTCCCCG 
CTCTGCTCAC CTACTGAAAC CCAGCCCACC ACCCATGGCT ACAGCCAGAC AACACCTCCG 
AGTGTGTCTC AGCTGCCCCC AGAGCCACCT GGGCCACCTG GCTTTCCACG GGTGCCCAGT 
GCTGGTGCAG ATGGGCCCCT GGCACTATAT GGCTGGGGTG CCCTCCCTGC TGAGAACATC 
TCCCTGTGCC GGATCTCCTC TGTCCCTGGG ACGTCTAGGG TTGAGCCAGG CCCCAGGACC 
CCTGGCACTG CAGTGGTAGA CCTCCGTACA GCTGTCAAGC CCACTCCCAT CATCCTCACT 
GACCAGGGCA TGGACCTCAC CTCTCTTGCT GTGGAAGCGA GGAAGTATGG TCTTGCCCTG 
GATCCAATCC CAGGACGGCA GTCGACCGCC GTGCAGCCCT TGGTTATCAA CCTCAATGCC 
CAGGAGCATA CCTTCCTTGC TACTGCCACC ACCGTGAGCA TCACCATGGC CTCGTCTGTG 
TTCATGGCTC AACAAAAGCA GCCTGTGGTC TATGGAGACC CCTACCAGAG CCGCCTTGAC 
TTTGGCCAGG GTGGGGGTAG CCCTGTGTGC CTGGCCCAGG TCAAACAAGT AGAGCAGGCT 
GTCCAGACAG CCCCATACCG AAGTGGGCCC CGGGGAAGAC CCAGGGAGGC CAAGTTTGCC 
AGATATAACC TACCCAACCA AGTAGCTCCT CTGGCCAGAA GAGACGTTTT GATCACTCAG 
ATGGGCACCG CCCAGAGCAT TGGCCTCAAG CCAGGCCCAG TGCCAGAGCC AGGTGCCGAG 
CCCCACCGGG CCACCCCTGC AGAGCTGCGG TCACATGCTC TGCCAGGTGC CAGGAAGCCA 
CACACAGTGG TGGTGCAGAT GGGAGAGGGC ACAGCAGGCA CTGTGACCAC ACTGCTCCCA 
GAGGAGCCTG CGGGTGCCCT GGACCTTACC GGGATGAGGC CTGAGAGCCA GCTGGCATGC 
TGTGACATGG TCTACAAGCT CCCCTTTGGC AGCAGCTGCA CTGGCACCTT CCACCCGGCC 
CCCAGTGTGC CTGAGAAGAG CATGGCAGAT GCTGCCCCAC CTGGCCAAAG CAGCAGCCCC 
TTCTATGGTC CCCGGGACCC TGAGCCTCCT GAGCCCCCAA CCTACCGGGC ACAGGGGGTG 
GTGGGGCCTG GGCCCCATGA GGAGCAGAGG CCCTACCCAC AAGGCCTGCC TGGTAGGCTG 
TACTCCTCCA TGTCTGACAC CAATTTGGCT GAGGCTGGCC TCAACTACCA TGCCCAGAGG 
ATCGGGCAGC TCTTCCAGGG TCCTGGACGA GACTCGGCTA TGGACCTCAG CTCACTGAAG 
CACTCCTACA GCCTGGGCTT TGCGGATGGA CGCTACCTAG GGCAGGGCTT GCAGTATGGC 
TCAGTCACGG ACCTGCGTCA TCCTACAGAC CTTTTGGCTC ACCCGCTTCC CATGCGGCGC 
TATAGCTCAG TGTCGAACAT CTACTCAGAC CACAGGTACG GCCCACGGGG AGATGCAGTT 
GGCTTCCAGG AGGCCAGCCT GGCCCAGTAC AGTGCCACCA CAGCCCGTGA AATCAGTCGC 
ATGTGCGCTG CCCTCAACTC CATGGACCAG TATGGTGGGC GGCATGGCAG TGGTGGTGGT 
GGCCCTGACC TTGTGCAGTA CCAGCCCCAG CACGGGCCCG GGCTCAGTGC TCCACAGAGT 
CTGGTTCCCC TCAGACCTGG ACTCCTTGGT AACCCCACCT TTCCAGAGGG CCACCCAAGT 
CCTGGGAACT TGGCCCAGTA TGGGCCTGCA GCAGGCCAAG GAACAGCAGT CAGACAGCTG 
CTGCCGTCCA CAGCCACTGT ACGTGCAGCT GATGGCATGA TCTACTCGAC TATCAATACC 
CCAATTGCTG CAACACTGCC CATCACCACC CAGCCTGCCT CAGTCCTGCG GCCCATGGTG 
CGTGGTGGCA TGTACAGGCC TTACGCATCT GGTGGAATCA CAGCCGTGCC ACTCACCAGT 
CTGACACGTG TGCCCATGAT TGCCCCCCGG GTACCTCTTG GACCCACAGG GCTGTACCGC 
TATCCTGCAC CAAGTAGATT TCCCATTGCT TCCAGTGTTC CACCTGCAGA AGGGCCTGTC 
TATCTGGGGA AACCTGCTGC TGCCAAGGCC CCTGGGGCTG GGGGCCCTTC AAGGCCAGAG 
ATGCCAGTAG GGGCTGCACG GGAAGAGCCT CTTCCCACAA CCACCCCTGC TGCCATCAAG 
GAGGCTGCAG GAGCCCCAGC TCCTGCCCCA CTAGCTGGCC AGAAGCCACC AGCAGATGCT 
GCTCCTGGGG GTGGCAGTGG GGCCCTCAGC CGGCCAGGGT TCGAGAAAGA GGAAGCATCA 
CAGGAGGAGA GGCAGCGGAA GCAACAGGAG CAGCTGCTCC AGCTAGAGCG GGAGCGGGTG 
GAGTTGGAGA AGCTGCGACA ACTTCGGCTG CAAGAGGAGC TAGAGCGGGA ACGTGTGGAG 
CTGCAGAGGC ACCGTGAGGA GGAGCAGCTG CTGGTGCAGC GGGAGTTGCA GGAGCTGCAG 
ACCATCAAGC ACCATGTGCT GCAGCAGCAG CAAGAGGAAC GCCAGGCTCA ATTTGCACTG 
CAGCGGGAAC AGCTAGCGCA GCAGCGTCTG CAGCTGGAGC AGATCCAGCA GCTGCAGCAG 
CAGCTGCAGC AGCAGCTAGA GGAGCAGAAG CAGCGGCAGA AGGCTCCCTT TCCTGCAGCC 
TGTGAGGCAC CTGGCCGAGG GCCTCCCCTA GCGGCTGCTG AGTTGGCCCA GAATGGCCAG 
TATTGGCCCC CCCTTACACA TGCAGCCTTC ATTGCCATGG CAGGGCCTGA AGGACTTGGG 
CAGCCTCGTG AGCCTGTGCT GCACCGGGGT CTCCCCAGCT CTGCCTCAGA CATGTCACTG 
CAAACGGAGG AGCAGTGGGA GGCCAGCCGT AGTGGCATCA AGAAGCGGCA CTCCATGCCA 
CGCCTGCGGG ATGCCTGTGA GCTAGAGTCT GGGACTGAGC CCTGTGTGGT CAGGAGGATT 
GCCGACAGCA GCGTGCAGAC AGACGATGAG GATGGGGAGA GCCGCTACCT CTTGAGTCGG 
CGACGCCGGG CACGGCGGAG TGCTGACTGC AGTGTGCAGA CGGACGACGA AGACAGTGCT 
GAGTGGGAGC AGCCAGTGCG CCGCCGCAGG TCTCGTCTTC CCCGCCACTC AGACTCAGGC 
TCTGACAGCA AGCACGATGC CACTGCCTCA TCATCCAGTG CTGCTGCCAC TGTGAGGGCC 
ATGAGCAGCG TGGGCATCCA GACCATCAGT GACTGCTCCG TGCAGACGGA GCCTGACCAG 
CTGCCCAGGG TCTCTCCAGC CATCCACATC ACAGCTGCCA CCGATCCCAA GGTGGAGATC 
GTCAGGTACA TATCGGCGCC AGAGAAGACT GGGCGTGGGG AGAGCCTGGC CTGCCAGACG 
GAGCCAGATG GGCAGGCCCA GGGTGTAGCC GGGCCGCAGC TTGTAGGGCC AACTGCCATC 
AGCCCCTACC TGCCTGGCAT CCAGATCGTC ACCCCAGGGC CTCTGGGCAG ATTTGAAAAA 
AAGAAGCCAG ATCCCCTGGA GATTGGGTAC CAGGCCCACC TGCCTCCGGA GTCTCTCTCA 
CAGCTTGTGA GCCGCCAGCC TCCCAAGTCC CCTCAGGTCC TCTACTCACC AGTCTCACCC 
CTGTCCCCTC ACCGGCTCCT GGACACCTCC TTTGCTTCCA GTGAGAGGCT GAACAAAGCT 
CACGTGAGTC CCCAGAAGCA CTTCACGGCT GACAGCGCTC TCCGCCAGCA GACGCTGCCT 
CGCCCCATGA AGACCCTGCA GCGGTCCCTG TCTGACCCTA AGCCCCTCAG CCCCACCGCC 
GAAGAGTCTG CCAAAGAGAG ATTCTCCCTC TACCAGCACC AGGGGGGACT GGGTAGCCAG 
GTGTCGGCGT TGCCACCCAA CAGCCTGGTC CGCAAGGTGA AGCGGACACT GCCCAGCCCC 
CCTCCAGAGG AGGCTCACCT TCCCCTGGCT GGCCAGGCCT CCCCACAGCT GTATGCAGCC 
AGCCTGCTGC AGCGAGGGCT GACGGGGCCC ACCACTGTCC CTGCTACCAA GGCCAGCCTG 
CTCCGGGAGC TGGACCGGGA CCTGCGGCTG GTGGAGCATG AGTCCACCAA ACTGCGCAAG 
AAGCAGGCAG AGCTGGATGA GGAGGAGAAG GAGATTGACG CCAAGCTCAA GTACCTGGAG 
TTGGGTATCA CACAACGCAA AGAGTCTTTG GCCAAAGACC GGGGTGGCCG TGACTACCCA 
CCCTTGCGTG GTCTTGGCGA GCATCGTGAC TACCTATCGG ACAGTGAGCT CAACCAGCTG 
CGGCTCCAGG GCTGCACCAC TCCCGCTGGC CAGTTTGTGG ACTTCCCTGC CACTGCCGCT 
GCTCCTGCCA CCCCCTCTGG TCCCACTGCC TTCCAGCAGC CCCGCTTCCA GCCTCCAGCC 
CCACAGTATT CTGCAGGCAG TGGTGGGCCA ACTCAGAACG GATTCCCAGC CCACCAGGCC 
CCCACCTACC CTGGCCCCAG CACGTACCCA GCTCCTGCCT TTCCTCCTGG TGCCAGTTAC 
CCAGCTGAGC CTGGCCTGCC AAACCAGCAG GCTTTCCGCC CCACAGGCCA CTATGCAGGC 
CAAACACCCA TGCCAACCAC ACAGAGCACC CTTTTTCCAG TCCCCGCTGA TAGCCGTGCC 
CCACTGCAGA AGCCACGCCA GACATCGCTA GCCGACTTGG AGCAGAAGGT GCCCACCAAC 
TATGAGGTGA TCGCCAGCCC CGTTGTGCCC ATGTCTTCAG CCCCATCTGA AACCAGCTAC 
AGTGGCCCAG CAGTGAGCAG CGGCTATGAG CAGGGCAAGG TCCCTGAGGT GCCCCGGGCT 
GGTGACCGTG GCAGTGTGAG CCAGAGCCCA GCCCCCACCT ACCCCTCTGA CTCACACTAT 
ACCAGTCTGG AGCAGAACGT TCCTCGAAAC TACGTAATGA TTGATGACAT CAGTGAACTG 
ACCAAGGACA GCACCTCTAC TGCTCCTGAT AGCCAACGGC TGGAGCCCCT GGGGCCAGGC 
AGCAGTGGGC GTCCAGGGAA GGAGCCTGGA GAACCAGGTG TCCTTGACGG GCCCACACTG 
CCCTGCTGCT ATGCCAGAGG AGAAGAGGAA TCTGAGGAGG ACTCATACGA TCCCCGCGGG 
AAGGGTGGCC ACCTCCGGAG CATGGAGAGC AATGGTCGAC CAGCCAGTAC CCACTACTAT 
GGTGACAGTG ACTACAGGCA TGGGGCTCGA GTAGAGAAGT ATGGTCCAGG GCCCATGGGG 
CCCAAGCATC CCTCCAAGAG CCTGGCTCCA GCTGCCATCT CCTCAAAGCG CAGCAAGCAC 
CGGAAGCAGG GCATGGAGCA AAAGATATCC AAGTTCTCGC CTATTGAAGA GGCCAAAGAC 
GTAGAGTCAG ACCTGGCGTC CTACCCCCCA CCTGCAGTCA GCAGCAGCCT GGTCTCTCGG 
GGCAGGAAGT TCCAGGATGA AATCACCTAT GGGCTCAAGA AGAACGTGTA TGAGCAGCAA 
AAATACTATG GGATGTCCAG CCGGGACGCA GTGGAGGACG ACCGCATTTA TGGCGGGAGC 
AGCCGGTCCC GGGCACCTTC TGCATACAGT GGGGAGAAGC TGTCCAGCCA CGACTTCAGT 
GGCTGGGGCA AGGGGTACGA AAGGGAACGG GAGGCTGTGG AGCGACTTCA AAAAGCGGGC 
CCCAAGCCCT CATCCCTAAG TATGGCCCAC AGCCGGGTAC GACCCCCCAT GCGGAGCCAG 
GCCTCTGAAG AGGAGAGCCC CGTCAGTCCT TTGGGGAGGC CCCGCCCTGC CGGAGGGCCC 
CTCCCTCCCG GCGGGGATAC CTGCCCACAG TTCTGCTCCA GCCACTCCAT GCCTGATGTC 
CAGGAACATG TCAAGGACGG ACCTCGGGCC CACGCATATA AGCGTGAGGA GGGCTACATC 
CTGGATGATT CCCATTGCGT GGTTTCCGAC AGCGAAGCGT ATCACCTGGG CCAGGAGGAG 
ACGGACTGGT TTGATAAGCC CCGGGATGCC CGCTCTGACC GGTTCAGGCA CCACGGGGGC 
CATGCAGTTT CCTCCTCCTC CCAGAAGCGA GGCCCTGCCA GGCACAGCTA CCATGACTAC 
GATGAACCCC CTGAGGAGGG CCTGTGGCCT CATGATGAGG GTGGCCCAGG CCGCCATGCC 
TCAGCCAAGG AACACCGGCA CGGTGACCAC GGGCGGCACT CAGGCCGCCA CACTGGTGAG 
GAGCCGGGAC GGCGTGCTGC CAAACCACAC GCTCGGGACC TGGGTCGCCA TGAGGCCCGG 
CCCCACTCTC AGCCCAGCTC TGCTCCAGCT ATGCCGAAGA AGGGTCAGCC TGGGTATCCC 
AGCTCTGCTG AGTACTCACA GCCATCCCGT GCTTCATCCG CATACCATCA TGCCTCTGAC 
AGCAAGAAGG GCTCCCGGCA AGCCCACTCC GGGCCCGCTG CACTGCAGTC AAAGGCAGAA 
CCCCAGGCGC AGCCGCAGCT GCAAGGTCGG CAGGCAGCTC CAGGACCACA GCAGTCACAG 
TCACCATCAT CCAGGCAAAT ACCCTCTGGG GCAGCATCAC GCCAGCCACA GACACAGCAG 
CAGCAGCAAG GTCTTGGGCT GCAGCCCCCA CAGCAGGCTC TGACACAGGC TCGGCTGCAG 
CAACAGAGCC AGCCAACCAC CCGGGGCTCA GCCCCTGCTG CCAGCCAGCC TGCAGGGAAG 
CCTCAGCCAG GCCCCAGCAC AGCCACAGGT CCTCAACCAG CAGGACCGCC ACGGGCAGAA 
CAGACAAATG GCTCTAAAGG GACAGCCAAA GCACCGCAAC AGGGGAGGGC TCCTCAGGCC 
CAGCCAGCAC CAGGACCTGG ACCTGCAGGT GTGAAGGCTG GAGCCAGGCC TGGAGGAACC 
CCAGGGGCTC CCGCCGGCCA GCCAGGTGCC GATGGGGAGA GCGTGTTCTC CAAGATCCTC 
CCTGGCGGGG CAGCCGAGCA AGCTGGCAAA CTGACGGAAG CTGTCTCTGC TTTTGGCAAA 
AAATTTTCCT CATTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in bassoon in individuals with familial and sporadic progressive supranuclear palsy-like syndrome.
Scientific reports8(1)819(2018 Jan)
Yabe I,Yaguchi H,Kato Y,Miki Y,Takahashi H,Tanikawa S,Shirai S,Takahashi I,Kimura M,Hama Y,Matsushima M,Fujioka S,Kano T,Watanabe M,Nakagawa S,Kunieda Y,Ikeda Y,Hasegawa M,Nishihara H,Ohtsuka T,Tanaka S,Tsuboi Y,Hatakeyama S,Wakabayashi K,Sasaki H

A genome-wide association study identifies a novel locus at 6q22.1 associated with ulcerative colitis.
Human molecular genetics23(25)6927-34(2014 Dec)
Juli? A,Dom?nech E,Chaparro M,Garc?a-S?nchez V,Gomoll?n F,Pan?s J,Ma?osa M,Barreiro-De Acosta M,Guti?rrez A,Garcia-Planella E,Aguas M,Mu?oz F,Esteve M,Mendoza JL,Vera M,M?rquez L,Tortosa R,L?pez-Lasanta M,Alonso A,Gelp? JL,Garc?a-Montero AC,Bertranpetit J,Absher D,Myers RM,Gisbert JP,Marsal S

Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
Nature491(7422)119-24(2012 Nov)
Jostins L,Ripke S,Weersma RK,Duerr RH,McGovern DP,Hui KY,Lee JC,Schumm LP,Sharma Y,Anderson CA,Essers J,Mitrovic M,Ning K,Cleynen I,Theatre E,Spain SL,Raychaudhuri S,Goyette P,Wei Z,Abraham C,Achkar JP,Ahmad T,Amininejad L,Ananthakrishnan AN,Andersen V,Andrews JM,Baidoo L,Balschun T,Bampton PA,Bitton A,Boucher G,Brand S,B?ning C,Cohain A,Cichon S,D'Amato M,De Jong D,Devaney KL,Dubinsky M,Edwards C,Ellinghaus D,Ferguson LR,Franchimont D,Fransen K,Gearry R,Georges M,Gieger C,Glas J,Haritunians T,Hart A,Hawkey C,Hedl M,Hu X,Karlsen TH,Kupcinskas L,Kugathasan S,Latiano A,Laukens D,Lawrance IC,Lees CW,Louis E,Mahy G,Mansfield J,Morgan AR,Mowat C,Newman W,Palmieri O,Ponsioen CY,Potocnik U,Prescott NJ,Regueiro M,Rotter JI,Russell RK,Sanderson JD,Sans M,Satsangi J,Schreiber S,Simms LA,Sventoraityte J,Targan SR,Taylor KD,Tremelling M,Verspaget HW,De Vos M,Wijmenga C,Wilson DC,Winkelmann J,Xavier RJ,Zeissig S,Zhang B,Zhang CK,Zhao H,,Silverberg MS,Annese V,Hakonarson H,Brant SR,Radford-Smith G,Mathew CG,Rioux JD,Schadt EE,Daly MJ,Franke A,Parkes M,Vermeire S,Barrett JC,Cho JH

D-amino acid oxidase activity is inhibited by an interaction with bassoon protein at the presynaptic active zone.
The Journal of biological chemistry286(33)28867-75(2011 Aug)
Popiolek M,Ross JF,Charych E,Chanda P,Gundelfinger ED,Moss SJ,Brandon NJ,Pausch MH

Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.
European archives of psychiatry and clinical neuroscience259(3)151-63(2009 Apr)
Martins-de-Souza D,Gattaz WF,Schmitt A,Rewerts C,Maccarrone G,Dias-Neto E,Turck CW

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BSN cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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