TIMELESS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMELESS
Entrez Gene ID	8914
Full Name	timeless circadian regulator
Synonyms	TIM,TIM1,hTIM
General protein information	Preferred Names timeless circadian regulator Names protein timeless homolog Tof1 homolog timeless circadian clock 1 timeless homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.3
Summary	The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. [provided by RefSeq, Feb 2014].
Disorder MIM:	603887

mRNA and Protein(s)

mRNA	Protein	Name
NM_001330295.1	NP_001317224.1	protein timeless homolog isoform 2
NM_003920.5	NP_003911.2	protein timeless homolog isoform 1
NM_001330295.2	NP_001317224.1	protein timeless homolog isoform 2
NM_003920.4	NP_003911.2	protein timeless homolog isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TIMELESS	NP_003911.2
Canis lupus familiaris (dog)	TIMELESS	XP_538233.3
Pan troglodytes (chimpanzee)	TIMELESS	XP_509535.2
	LOC712835	XP_002808027.1
Danio rerio (zebrafish)	timeless	NP_001265529.1
Xenopus tropicalis (tropical clawed frog)	timeless	XP_002934575.1
Bos taurus (cattle)	TIMELESS	NP_001095541.2
Mus musculus (house mouse)	Timeless	NP_035719.1
Rattus norvegicus (Norway rat)	Timeless	NP_112630.1
Drosophila melanogaster (fruit fly)	timeout	NP_524341.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010787	XP_001689129.1

Gene Ontology

The following TIMELESS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMELESS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26255	NM_003920.5 Latest version!	Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu62315	NM_001330295.2 Latest version!	Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu62315	NM_001330295.1	Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu26255	NM_003920.4	Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMELESS ( NM_003920.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003911.2 Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26255
Clone ID Related Accession (Same CDS sequence)	NM_003920.5 , NM_003920.4
Accession Version	NM_003920.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	protein timeless homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC024884.31. On Nov 23, 2018 this sequence version replaced NM_003920.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.86329.1, BC050557.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000553532.6/ ENSP00000450607.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACTTGC ACATGATGAA CTGTGAACTT CTAGCCACAT GTAGTGCCCT TGGGTACTTG 
GAGGGAGACA CTTACCATAA GGAACCAGAT TGCTTAGAGA GCGTGAAGGA TCTGATCCGC 
TATTTGAGGC ATGAGGATGA GACACGAGAT GTGCGGCAGC AGCTGGGGGC AGCCCAGATC 
CTACAGAGCG ACCTTCTGCC CATCCTCACC CAGCACCACC AGGACAAGCC TCTCTTTGAT 
GCTGTTATCA GACTGATGGT GAACTTGACA CAACCAGCCT TGCTCTGTTT TGGCAATCTG 
CCTAAGGAGC CCAGCTTTCG GCACCATTTT TTGCAGGTGC TAACTTATTT GCAGGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCTTTT GGAGTCCTCA GTGAAACCTT GTATGAGCTG 
CTGCAGCTGG GCTGGGAGGA ACGGCAGGAG GAAGACAACT TGCTGATTGA ACGGATCCTA 
CTGCTGGTCA GAAATATTCT CCATGTCCCA GCTGACCTTG ATCAGGAGAA GAAGATTGAT 
GATGACGCCA GTGCCCATGA CCAGCTCCTC TGGGCGATTC ACCTCAGCGG CCTGGATGAC 
CTGCTCCTCT TTCTGGCCAG CTCGTCTGCT GAGGAGCAAT GGAGCCTACA TGTGCTAGAG 
ATTGTCTCCC TTATGTTTCG TGACCAGAAC CCCGAGCAGC TGGCGGGAGT AGGGCAGGGA 
CGCTTAGCTC AGGAGCGGAG TGCAGATTTT GCAGAACTGG AGGTGTTGCG CCAGCGAGAG 
ATGGCAGAAA AGAAGACTCG AGCCCTCCAG CGAGGCAACA GGCATTCTCG ATTTGGGGGC 
TCCTATATTG TCCAGGGGTT GAAATCCATT GGGGAGAGGG ACCTCATCTT TCACAAAGGC 
CTTCACAACC TACGAAACTA CAGTTCAGAT TTGGGAAAGC AGCCGAAAAA GGTGCCTAAA 
CGTCGCCAGG CCGCCCGAGA GCTGTCCATT CAGCGCCGTT CTGCCCTCAA TGTGAGGCTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGTT ACAACCGGCT CATGGGATCA 
GTAAAGGATC ACCTGCTTCG GGAGAAAGCT CAGCAGCATG ATGAGACCTA TTATATGTGG 
GCCTTGGCTT TCTTCATGGC CTTCAACCGA GCTGCCTCCT TCCGGCCAGG CCTGGTTTCT 
GAGACCCTCA GTGTCCGTAC CTTCCACTTC ATTGAGCAGA ACCTCACCAA CTACTATGAG 
ATGATGCTGA CTGACCGCAA GGAAGCTGCC TCCTGGGCAC GCCGGATGCA CTTGGCTCTG 
AAGGCCTATC AGGAGCTGCT GGCAACAGTG AATGAGATGG ACATATCTCC AGATGAGGCT 
GTGAGGGAGA GCAGCCGCAT CATCAAGAAC AATATTTTCT ATGTGATGGA GTACCGAGAA 
CTATTCCTGG CACTTTTTCG AAAGTTTGAT GAGAGATGCC AGCCCCGCTC TTTCCTTCGT 
GACCTGGTGG AGACCACCCA CCTCTTCCTC AAAATGTTGG AGCGATTCTG TCGGAGCCGT 
GGGAACCTGG TGGTGCAGAA CAAACAAAAG AAGAGAAGGA AGAAGAAGAA GAAGGTCCTA 
GACCAGGCCA TTGTTTCTGG TAATGTCCCA TCTAGCCCAG AAGAAGTGGA GGCTGTGTGG 
CCAGCCCTGG CTGAGCAGCT ACAGTGCTGT GCCCAGAATT CTGAGCTCAG CATGGACTCC 
GTGGTTCCCT TTGATGCGGC CTCAGAGGTG CCAGTGGAGG AGCAGCGGGC AGAAGCTATG 
GTACGGATCC AAGACTGTCT CCTGGCTGGC CAGGCCCCAC AGGCCCTGAC TCTCCTGAGG 
TCTGCTCGGG AGGTGTGGCC TGAAGGAGAT GTGTTTGGCT CTCAAGACAT TTCTCCAGAG 
GAAGAGATCC AGTTGCTGAA ACAAATCCTC TCTGCTCCAC TTCCCCGGCA GCAGGGCCCA 
GAGGAACGTG GGGCAGAGGA AGAAGAAGAG GAGGAGGAGG AGGAAGAGGA GGAGTTGCAA 
GTGGTCCAGG TGTCGGAGAA AGAATTTAAT TTTCTGGACT ACCTGAAACG CTTTGCATGT 
TCAACTGTCG TTCGAGCCTA TGTGCTGCTA CTAAGGAGCT ACCAGCAGAA TAGTGCCCAC 
ACTAACCATT GCATTGTGAA GATGCTGCAC CGGCTGGCCC ATGACCTCAA AATGGAAGCC 
CTACTTTTTC AGCTGTCAGT CTTCTGCCTC TTCAATCGTC TGCTTAGTGA CCCTGCTGCT 
GGAGCCTACA AAGAGCTAGT GACTTTTGCC AAATACATCC TGGGCAAATT TTTTGCACTG 
GCTGCAGTCA ACCAAAAAGC CTTTGTGGAG CTGTTGTTCT GGAAGAACAC AGCTGTGGTT 
CGAGAGATGA CTGAGGGCTA TGGCTCCCTG GATGACAGGT CTTCCAGTCG CAGAGCACCT 
ACATGGAGCC CCGAAGAAGA GGCTCATCTT CGGGAGCTGT ACCTCGCCAA TAAGGACGTG 
GAAGGGCAGG ATGTGGTGGA AGCCATCTTG GCCCACCTGA ATACTGTTCC TCGAACACGC 
AAGCAGATCA TCCACCATCT GGTACAGATG GGACTGGCTG ACAGTGTCAA GGACTTCCAA 
AGGAAAGGAA CCCATATTGT ACTGTGGACG GGGGATCAGG AGTTGGAGCT GCAGCGGCTT 
TTTGAGGAAT TCCGGGACTC AGATGATGTC CTGGGTCATA TCATGAAGAA TATCACAGCC 
AAACGCTCAC GGGCCCGAAT AGTGGATAAA CTCTTGGCTC TGGGGCTGGT GGCTGAGCGG 
CGGGAGCTGT ACAAGAAACG GCAGAAAAAG TTGGCATCCT CCATCTTGCC AAATGGAGCG 
GAGTCCCTGA AAGATTTTTG CCAGGAAGAT CTGGAAGAAG AGGAAAACCT GCCTGAGGAA 
GACAGCGAAG AGGAAGAAGA AGGGGGCTCA GAAGCAGAAC AAGTCCAGGG TAGCTTAGTC 
CTTTCAAATG AAAACCTTGG TCAAAGCCTG CATCAGGAAG GCTTTTCTAT CCCGCTCCTA 
TGGCTCCAGA ACTGCCTGAT CCGAGCAGCT GATGATCGGG AAGAGGATGG CTGCTCCCAG 
GCCGTTCCAT TGGTGCCACT CACAGAGGAA AATGAGGAAG CCATGGAAAA CGAACAGTTT 
CAGCAGCTGT TGCGCAAGCT AGGGGTTCGG CCCCCTGCCT CTGGGCAGGA AACCTTCTGG 
CGAATTCCAG CCAAGCTGAG TCCTACCCAG CTCCGGAGGG CAGCAGCTTC TTTGAGTCAA 
CCAGAGGAGG AACAGAAGCT GCAGCCAGAG CTGCAGCCTA AAGTCCCTGG AGAGCAAGGC 
TCTGATGAGG AGCACTGTAA AGAGCACCGA GCACAAGCCC TGAGGGCCCT CTTGCTAGCC 
CACAAGAAGA AAGCGGGCCT GGCATCCCCA GAGGAGGAAG ACGCTGTTGG TAAAGAGCCG 
CTGAAGGCAG CACCCAAGAA ACGACAATTG CTGGACAGCG ACGAGGAACA GGAAGAAGAT 
GAGGGCAGGA ACAGAGCACC AGAGTTGGGA GCTCCAGGAA TCCAAAAGAA GAAACGATAC 
CAGATTGAGG ATGATGAGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003911.2
CDS	179..3805
Translation	MDLHMMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHHQDKPLFDAVIRLMVNLTQPALLCFGNLPKEPSFRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNILHVPADLDQEKKIDDDASAHDQLLWAIHLSGLDDLLLFLASSSAEEQWSLHVLEIVSLMFRDQNPEQLAGVGQGRLAQERSADFAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIVQGLKSIGERDLIFHKGLHNLRNYSSDLGKQPKKVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREKAQQHDETYYMWALAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDISPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRSRGNLVVQNKQKKRRKKKKKVLDQAIVSGNVPSSPEEVEAVWPALAEQLQCCAQNSELSMDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALTLLRSAREVWPEGDVFGSQDISPEEEIQLLKQILSAPLPRQQGPEERGAEEEEEEEEEEEEELQVVQVSEKEFNFLDYLKRFACSTVVRAYVLLLRSYQQNSAHTNHCIVKMLHRLAHDLKMEALLFQLSVFCLFNRLLSDPAAGAYKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGSLDDRSSSRRAPTWSPEEEAHLRELYLANKDVEGQDVVEAILAHLNTVPRTRKQIIHHLVQMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEEDSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSIPLLWLQNCLIRAADDREEDGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQGSDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRYQIEDDEDD

Target ORF information:

RefSeq Version	NM_003920.5
Organism	Homo sapiens(human)
Definition	Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003920.5

ORF Insert Sequence:

ATGGACTTGC ACATGATGAA CTGTGAACTT CTAGCCACAT GTAGTGCCCT TGGGTACTTG 
GAGGGAGACA CTTACCATAA GGAACCAGAT TGCTTAGAGA GCGTGAAGGA TCTGATCCGC 
TATTTGAGGC ATGAGGATGA GACACGAGAT GTGCGGCAGC AGCTGGGGGC AGCCCAGATC 
CTACAGAGCG ACCTTCTGCC CATCCTCACC CAGCACCACC AGGACAAGCC TCTCTTTGAT 
GCTGTTATCA GACTGATGGT GAACTTGACA CAACCAGCCT TGCTCTGTTT TGGCAATCTG 
CCTAAGGAGC CCAGCTTTCG GCACCATTTT TTGCAGGTGC TAACTTATTT GCAGGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCTTTT GGAGTCCTCA GTGAAACCTT GTATGAGCTG 
CTGCAGCTGG GCTGGGAGGA ACGGCAGGAG GAAGACAACT TGCTGATTGA ACGGATCCTA 
CTGCTGGTCA GAAATATTCT CCATGTCCCA GCTGACCTTG ATCAGGAGAA GAAGATTGAT 
GATGACGCCA GTGCCCATGA CCAGCTCCTC TGGGCGATTC ACCTCAGCGG CCTGGATGAC 
CTGCTCCTCT TTCTGGCCAG CTCGTCTGCT GAGGAGCAAT GGAGCCTACA TGTGCTAGAG 
ATTGTCTCCC TTATGTTTCG TGACCAGAAC CCCGAGCAGC TGGCGGGAGT AGGGCAGGGA 
CGCTTAGCTC AGGAGCGGAG TGCAGATTTT GCAGAACTGG AGGTGTTGCG CCAGCGAGAG 
ATGGCAGAAA AGAAGACTCG AGCCCTCCAG CGAGGCAACA GGCATTCTCG ATTTGGGGGC 
TCCTATATTG TCCAGGGGTT GAAATCCATT GGGGAGAGGG ACCTCATCTT TCACAAAGGC 
CTTCACAACC TACGAAACTA CAGTTCAGAT TTGGGAAAGC AGCCGAAAAA GGTGCCTAAA 
CGTCGCCAGG CCGCCCGAGA GCTGTCCATT CAGCGCCGTT CTGCCCTCAA TGTGAGGCTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGTT ACAACCGGCT CATGGGATCA 
GTAAAGGATC ACCTGCTTCG GGAGAAAGCT CAGCAGCATG ATGAGACCTA TTATATGTGG 
GCCTTGGCTT TCTTCATGGC CTTCAACCGA GCTGCCTCCT TCCGGCCAGG CCTGGTTTCT 
GAGACCCTCA GTGTCCGTAC CTTCCACTTC ATTGAGCAGA ACCTCACCAA CTACTATGAG 
ATGATGCTGA CTGACCGCAA GGAAGCTGCC TCCTGGGCAC GCCGGATGCA CTTGGCTCTG 
AAGGCCTATC AGGAGCTGCT GGCAACAGTG AATGAGATGG ACATATCTCC AGATGAGGCT 
GTGAGGGAGA GCAGCCGCAT CATCAAGAAC AATATTTTCT ATGTGATGGA GTACCGAGAA 
CTATTCCTGG CACTTTTTCG AAAGTTTGAT GAGAGATGCC AGCCCCGCTC TTTCCTTCGT 
GACCTGGTGG AGACCACCCA CCTCTTCCTC AAAATGTTGG AGCGATTCTG TCGGAGCCGT 
GGGAACCTGG TGGTGCAGAA CAAACAAAAG AAGAGAAGGA AGAAGAAGAA GAAGGTCCTA 
GACCAGGCCA TTGTTTCTGG TAATGTCCCA TCTAGCCCAG AAGAAGTGGA GGCTGTGTGG 
CCAGCCCTGG CTGAGCAGCT ACAGTGCTGT GCCCAGAATT CTGAGCTCAG CATGGACTCC 
GTGGTTCCCT TTGATGCGGC CTCAGAGGTG CCAGTGGAGG AGCAGCGGGC AGAAGCTATG 
GTACGGATCC AAGACTGTCT CCTGGCTGGC CAGGCCCCAC AGGCCCTGAC TCTCCTGAGG 
TCTGCTCGGG AGGTGTGGCC TGAAGGAGAT GTGTTTGGCT CTCAAGACAT TTCTCCAGAG 
GAAGAGATCC AGTTGCTGAA ACAAATCCTC TCTGCTCCAC TTCCCCGGCA GCAGGGCCCA 
GAGGAACGTG GGGCAGAGGA AGAAGAAGAG GAGGAGGAGG AGGAAGAGGA GGAGTTGCAA 
GTGGTCCAGG TGTCGGAGAA AGAATTTAAT TTTCTGGACT ACCTGAAACG CTTTGCATGT 
TCAACTGTCG TTCGAGCCTA TGTGCTGCTA CTAAGGAGCT ACCAGCAGAA TAGTGCCCAC 
ACTAACCATT GCATTGTGAA GATGCTGCAC CGGCTGGCCC ATGACCTCAA AATGGAAGCC 
CTACTTTTTC AGCTGTCAGT CTTCTGCCTC TTCAATCGTC TGCTTAGTGA CCCTGCTGCT 
GGAGCCTACA AAGAGCTAGT GACTTTTGCC AAATACATCC TGGGCAAATT TTTTGCACTG 
GCTGCAGTCA ACCAAAAAGC CTTTGTGGAG CTGTTGTTCT GGAAGAACAC AGCTGTGGTT 
CGAGAGATGA CTGAGGGCTA TGGCTCCCTG GATGACAGGT CTTCCAGTCG CAGAGCACCT 
ACATGGAGCC CCGAAGAAGA GGCTCATCTT CGGGAGCTGT ACCTCGCCAA TAAGGACGTG 
GAAGGGCAGG ATGTGGTGGA AGCCATCTTG GCCCACCTGA ATACTGTTCC TCGAACACGC 
AAGCAGATCA TCCACCATCT GGTACAGATG GGACTGGCTG ACAGTGTCAA GGACTTCCAA 
AGGAAAGGAA CCCATATTGT ACTGTGGACG GGGGATCAGG AGTTGGAGCT GCAGCGGCTT 
TTTGAGGAAT TCCGGGACTC AGATGATGTC CTGGGTCATA TCATGAAGAA TATCACAGCC 
AAACGCTCAC GGGCCCGAAT AGTGGATAAA CTCTTGGCTC TGGGGCTGGT GGCTGAGCGG 
CGGGAGCTGT ACAAGAAACG GCAGAAAAAG TTGGCATCCT CCATCTTGCC AAATGGAGCG 
GAGTCCCTGA AAGATTTTTG CCAGGAAGAT CTGGAAGAAG AGGAAAACCT GCCTGAGGAA 
GACAGCGAAG AGGAAGAAGA AGGGGGCTCA GAAGCAGAAC AAGTCCAGGG TAGCTTAGTC 
CTTTCAAATG AAAACCTTGG TCAAAGCCTG CATCAGGAAG GCTTTTCTAT CCCGCTCCTA 
TGGCTCCAGA ACTGCCTGAT CCGAGCAGCT GATGATCGGG AAGAGGATGG CTGCTCCCAG 
GCCGTTCCAT TGGTGCCACT CACAGAGGAA AATGAGGAAG CCATGGAAAA CGAACAGTTT 
CAGCAGCTGT TGCGCAAGCT AGGGGTTCGG CCCCCTGCCT CTGGGCAGGA AACCTTCTGG 
CGAATTCCAG CCAAGCTGAG TCCTACCCAG CTCCGGAGGG CAGCAGCTTC TTTGAGTCAA 
CCAGAGGAGG AACAGAAGCT GCAGCCAGAG CTGCAGCCTA AAGTCCCTGG AGAGCAAGGC 
TCTGATGAGG AGCACTGTAA AGAGCACCGA GCACAAGCCC TGAGGGCCCT CTTGCTAGCC 
CACAAGAAGA AAGCGGGCCT GGCATCCCCA GAGGAGGAAG ACGCTGTTGG TAAAGAGCCG 
CTGAAGGCAG CACCCAAGAA ACGACAATTG CTGGACAGCG ACGAGGAACA GGAAGAAGAT 
GAGGGCAGGA ACAGAGCACC AGAGTTGGGA GCTCCAGGAA TCCAAAAGAA GAAACGATAC 
CAGATTGAGG ATGATGAGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMELESS ( NM_003920.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003911.2 Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26255
Clone ID Related Accession (Same CDS sequence)	NM_003920.5 , NM_003920.4
Accession Version	NM_003920.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Homo sapiens(human)
Product	protein timeless homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY040179.1, AF098162.1, BC031514.1, AK000721.1 and BE503292.1. On Aug 23, 2016 this sequence version replaced NM_003920.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.86329.1, BC050557.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTTGC ACATGATGAA CTGTGAACTT CTAGCCACAT GTAGTGCCCT TGGGTACTTG 
GAGGGAGACA CTTACCATAA GGAACCAGAT TGCTTAGAGA GCGTGAAGGA TCTGATCCGC 
TATTTGAGGC ATGAGGATGA GACACGAGAT GTGCGGCAGC AGCTGGGGGC AGCCCAGATC 
CTACAGAGCG ACCTTCTGCC CATCCTCACC CAGCACCACC AGGACAAGCC TCTCTTTGAT 
GCTGTTATCA GACTGATGGT GAACTTGACA CAACCAGCCT TGCTCTGTTT TGGCAATCTG 
CCTAAGGAGC CCAGCTTTCG GCACCATTTT TTGCAGGTGC TAACTTATTT GCAGGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCTTTT GGAGTCCTCA GTGAAACCTT GTATGAGCTG 
CTGCAGCTGG GCTGGGAGGA ACGGCAGGAG GAAGACAACT TGCTGATTGA ACGGATCCTA 
CTGCTGGTCA GAAATATTCT CCATGTCCCA GCTGACCTTG ATCAGGAGAA GAAGATTGAT 
GATGACGCCA GTGCCCATGA CCAGCTCCTC TGGGCGATTC ACCTCAGCGG CCTGGATGAC 
CTGCTCCTCT TTCTGGCCAG CTCGTCTGCT GAGGAGCAAT GGAGCCTACA TGTGCTAGAG 
ATTGTCTCCC TTATGTTTCG TGACCAGAAC CCCGAGCAGC TGGCGGGAGT AGGGCAGGGA 
CGCTTAGCTC AGGAGCGGAG TGCAGATTTT GCAGAACTGG AGGTGTTGCG CCAGCGAGAG 
ATGGCAGAAA AGAAGACTCG AGCCCTCCAG CGAGGCAACA GGCATTCTCG ATTTGGGGGC 
TCCTATATTG TCCAGGGGTT GAAATCCATT GGGGAGAGGG ACCTCATCTT TCACAAAGGC 
CTTCACAACC TACGAAACTA CAGTTCAGAT TTGGGAAAGC AGCCGAAAAA GGTGCCTAAA 
CGTCGCCAGG CCGCCCGAGA GCTGTCCATT CAGCGCCGTT CTGCCCTCAA TGTGAGGCTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGTT ACAACCGGCT CATGGGATCA 
GTAAAGGATC ACCTGCTTCG GGAGAAAGCT CAGCAGCATG ATGAGACCTA TTATATGTGG 
GCCTTGGCTT TCTTCATGGC CTTCAACCGA GCTGCCTCCT TCCGGCCAGG CCTGGTTTCT 
GAGACCCTCA GTGTCCGTAC CTTCCACTTC ATTGAGCAGA ACCTCACCAA CTACTATGAG 
ATGATGCTGA CTGACCGCAA GGAAGCTGCC TCCTGGGCAC GCCGGATGCA CTTGGCTCTG 
AAGGCCTATC AGGAGCTGCT GGCAACAGTG AATGAGATGG ACATATCTCC AGATGAGGCT 
GTGAGGGAGA GCAGCCGCAT CATCAAGAAC AATATTTTCT ATGTGATGGA GTACCGAGAA 
CTATTCCTGG CACTTTTTCG AAAGTTTGAT GAGAGATGCC AGCCCCGCTC TTTCCTTCGT 
GACCTGGTGG AGACCACCCA CCTCTTCCTC AAAATGTTGG AGCGATTCTG TCGGAGCCGT 
GGGAACCTGG TGGTGCAGAA CAAACAAAAG AAGAGAAGGA AGAAGAAGAA GAAGGTCCTA 
GACCAGGCCA TTGTTTCTGG TAATGTCCCA TCTAGCCCAG AAGAAGTGGA GGCTGTGTGG 
CCAGCCCTGG CTGAGCAGCT ACAGTGCTGT GCCCAGAATT CTGAGCTCAG CATGGACTCC 
GTGGTTCCCT TTGATGCGGC CTCAGAGGTG CCAGTGGAGG AGCAGCGGGC AGAAGCTATG 
GTACGGATCC AAGACTGTCT CCTGGCTGGC CAGGCCCCAC AGGCCCTGAC TCTCCTGAGG 
TCTGCTCGGG AGGTGTGGCC TGAAGGAGAT GTGTTTGGCT CTCAAGACAT TTCTCCAGAG 
GAAGAGATCC AGTTGCTGAA ACAAATCCTC TCTGCTCCAC TTCCCCGGCA GCAGGGCCCA 
GAGGAACGTG GGGCAGAGGA AGAAGAAGAG GAGGAGGAGG AGGAAGAGGA GGAGTTGCAA 
GTGGTCCAGG TGTCGGAGAA AGAATTTAAT TTTCTGGACT ACCTGAAACG CTTTGCATGT 
TCAACTGTCG TTCGAGCCTA TGTGCTGCTA CTAAGGAGCT ACCAGCAGAA TAGTGCCCAC 
ACTAACCATT GCATTGTGAA GATGCTGCAC CGGCTGGCCC ATGACCTCAA AATGGAAGCC 
CTACTTTTTC AGCTGTCAGT CTTCTGCCTC TTCAATCGTC TGCTTAGTGA CCCTGCTGCT 
GGAGCCTACA AAGAGCTAGT GACTTTTGCC AAATACATCC TGGGCAAATT TTTTGCACTG 
GCTGCAGTCA ACCAAAAAGC CTTTGTGGAG CTGTTGTTCT GGAAGAACAC AGCTGTGGTT 
CGAGAGATGA CTGAGGGCTA TGGCTCCCTG GATGACAGGT CTTCCAGTCG CAGAGCACCT 
ACATGGAGCC CCGAAGAAGA GGCTCATCTT CGGGAGCTGT ACCTCGCCAA TAAGGACGTG 
GAAGGGCAGG ATGTGGTGGA AGCCATCTTG GCCCACCTGA ATACTGTTCC TCGAACACGC 
AAGCAGATCA TCCACCATCT GGTACAGATG GGACTGGCTG ACAGTGTCAA GGACTTCCAA 
AGGAAAGGAA CCCATATTGT ACTGTGGACG GGGGATCAGG AGTTGGAGCT GCAGCGGCTT 
TTTGAGGAAT TCCGGGACTC AGATGATGTC CTGGGTCATA TCATGAAGAA TATCACAGCC 
AAACGCTCAC GGGCCCGAAT AGTGGATAAA CTCTTGGCTC TGGGGCTGGT GGCTGAGCGG 
CGGGAGCTGT ACAAGAAACG GCAGAAAAAG TTGGCATCCT CCATCTTGCC AAATGGAGCG 
GAGTCCCTGA AAGATTTTTG CCAGGAAGAT CTGGAAGAAG AGGAAAACCT GCCTGAGGAA 
GACAGCGAAG AGGAAGAAGA AGGGGGCTCA GAAGCAGAAC AAGTCCAGGG TAGCTTAGTC 
CTTTCAAATG AAAACCTTGG TCAAAGCCTG CATCAGGAAG GCTTTTCTAT CCCGCTCCTA 
TGGCTCCAGA ACTGCCTGAT CCGAGCAGCT GATGATCGGG AAGAGGATGG CTGCTCCCAG 
GCCGTTCCAT TGGTGCCACT CACAGAGGAA AATGAGGAAG CCATGGAAAA CGAACAGTTT 
CAGCAGCTGT TGCGCAAGCT AGGGGTTCGG CCCCCTGCCT CTGGGCAGGA AACCTTCTGG 
CGAATTCCAG CCAAGCTGAG TCCTACCCAG CTCCGGAGGG CAGCAGCTTC TTTGAGTCAA 
CCAGAGGAGG AACAGAAGCT GCAGCCAGAG CTGCAGCCTA AAGTCCCTGG AGAGCAAGGC 
TCTGATGAGG AGCACTGTAA AGAGCACCGA GCACAAGCCC TGAGGGCCCT CTTGCTAGCC 
CACAAGAAGA AAGCGGGCCT GGCATCCCCA GAGGAGGAAG ACGCTGTTGG TAAAGAGCCG 
CTGAAGGCAG CACCCAAGAA ACGACAATTG CTGGACAGCG ACGAGGAACA GGAAGAAGAT 
GAGGGCAGGA ACAGAGCACC AGAGTTGGGA GCTCCAGGAA TCCAAAAGAA GAAACGATAC 
CAGATTGAGG ATGATGAGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003911.2
CDS	218..3844
Translation	MDLHMMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHHQDKPLFDAVIRLMVNLTQPALLCFGNLPKEPSFRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNILHVPADLDQEKKIDDDASAHDQLLWAIHLSGLDDLLLFLASSSAEEQWSLHVLEIVSLMFRDQNPEQLAGVGQGRLAQERSADFAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIVQGLKSIGERDLIFHKGLHNLRNYSSDLGKQPKKVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREKAQQHDETYYMWALAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDISPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRSRGNLVVQNKQKKRRKKKKKVLDQAIVSGNVPSSPEEVEAVWPALAEQLQCCAQNSELSMDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALTLLRSAREVWPEGDVFGSQDISPEEEIQLLKQILSAPLPRQQGPEERGAEEEEEEEEEEEEELQVVQVSEKEFNFLDYLKRFACSTVVRAYVLLLRSYQQNSAHTNHCIVKMLHRLAHDLKMEALLFQLSVFCLFNRLLSDPAAGAYKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGSLDDRSSSRRAPTWSPEEEAHLRELYLANKDVEGQDVVEAILAHLNTVPRTRKQIIHHLVQMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEEDSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSIPLLWLQNCLIRAADDREEDGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQGSDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRYQIEDDEDD

Target ORF information:

RefSeq Version	NM_003920.4
Organism	Homo sapiens(human)
Definition	Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003920.4

ORF Insert Sequence:

ATGGACTTGC ACATGATGAA CTGTGAACTT CTAGCCACAT GTAGTGCCCT TGGGTACTTG 
GAGGGAGACA CTTACCATAA GGAACCAGAT TGCTTAGAGA GCGTGAAGGA TCTGATCCGC 
TATTTGAGGC ATGAGGATGA GACACGAGAT GTGCGGCAGC AGCTGGGGGC AGCCCAGATC 
CTACAGAGCG ACCTTCTGCC CATCCTCACC CAGCACCACC AGGACAAGCC TCTCTTTGAT 
GCTGTTATCA GACTGATGGT GAACTTGACA CAACCAGCCT TGCTCTGTTT TGGCAATCTG 
CCTAAGGAGC CCAGCTTTCG GCACCATTTT TTGCAGGTGC TAACTTATTT GCAGGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCTTTT GGAGTCCTCA GTGAAACCTT GTATGAGCTG 
CTGCAGCTGG GCTGGGAGGA ACGGCAGGAG GAAGACAACT TGCTGATTGA ACGGATCCTA 
CTGCTGGTCA GAAATATTCT CCATGTCCCA GCTGACCTTG ATCAGGAGAA GAAGATTGAT 
GATGACGCCA GTGCCCATGA CCAGCTCCTC TGGGCGATTC ACCTCAGCGG CCTGGATGAC 
CTGCTCCTCT TTCTGGCCAG CTCGTCTGCT GAGGAGCAAT GGAGCCTACA TGTGCTAGAG 
ATTGTCTCCC TTATGTTTCG TGACCAGAAC CCCGAGCAGC TGGCGGGAGT AGGGCAGGGA 
CGCTTAGCTC AGGAGCGGAG TGCAGATTTT GCAGAACTGG AGGTGTTGCG CCAGCGAGAG 
ATGGCAGAAA AGAAGACTCG AGCCCTCCAG CGAGGCAACA GGCATTCTCG ATTTGGGGGC 
TCCTATATTG TCCAGGGGTT GAAATCCATT GGGGAGAGGG ACCTCATCTT TCACAAAGGC 
CTTCACAACC TACGAAACTA CAGTTCAGAT TTGGGAAAGC AGCCGAAAAA GGTGCCTAAA 
CGTCGCCAGG CCGCCCGAGA GCTGTCCATT CAGCGCCGTT CTGCCCTCAA TGTGAGGCTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGTT ACAACCGGCT CATGGGATCA 
GTAAAGGATC ACCTGCTTCG GGAGAAAGCT CAGCAGCATG ATGAGACCTA TTATATGTGG 
GCCTTGGCTT TCTTCATGGC CTTCAACCGA GCTGCCTCCT TCCGGCCAGG CCTGGTTTCT 
GAGACCCTCA GTGTCCGTAC CTTCCACTTC ATTGAGCAGA ACCTCACCAA CTACTATGAG 
ATGATGCTGA CTGACCGCAA GGAAGCTGCC TCCTGGGCAC GCCGGATGCA CTTGGCTCTG 
AAGGCCTATC AGGAGCTGCT GGCAACAGTG AATGAGATGG ACATATCTCC AGATGAGGCT 
GTGAGGGAGA GCAGCCGCAT CATCAAGAAC AATATTTTCT ATGTGATGGA GTACCGAGAA 
CTATTCCTGG CACTTTTTCG AAAGTTTGAT GAGAGATGCC AGCCCCGCTC TTTCCTTCGT 
GACCTGGTGG AGACCACCCA CCTCTTCCTC AAAATGTTGG AGCGATTCTG TCGGAGCCGT 
GGGAACCTGG TGGTGCAGAA CAAACAAAAG AAGAGAAGGA AGAAGAAGAA GAAGGTCCTA 
GACCAGGCCA TTGTTTCTGG TAATGTCCCA TCTAGCCCAG AAGAAGTGGA GGCTGTGTGG 
CCAGCCCTGG CTGAGCAGCT ACAGTGCTGT GCCCAGAATT CTGAGCTCAG CATGGACTCC 
GTGGTTCCCT TTGATGCGGC CTCAGAGGTG CCAGTGGAGG AGCAGCGGGC AGAAGCTATG 
GTACGGATCC AAGACTGTCT CCTGGCTGGC CAGGCCCCAC AGGCCCTGAC TCTCCTGAGG 
TCTGCTCGGG AGGTGTGGCC TGAAGGAGAT GTGTTTGGCT CTCAAGACAT TTCTCCAGAG 
GAAGAGATCC AGTTGCTGAA ACAAATCCTC TCTGCTCCAC TTCCCCGGCA GCAGGGCCCA 
GAGGAACGTG GGGCAGAGGA AGAAGAAGAG GAGGAGGAGG AGGAAGAGGA GGAGTTGCAA 
GTGGTCCAGG TGTCGGAGAA AGAATTTAAT TTTCTGGACT ACCTGAAACG CTTTGCATGT 
TCAACTGTCG TTCGAGCCTA TGTGCTGCTA CTAAGGAGCT ACCAGCAGAA TAGTGCCCAC 
ACTAACCATT GCATTGTGAA GATGCTGCAC CGGCTGGCCC ATGACCTCAA AATGGAAGCC 
CTACTTTTTC AGCTGTCAGT CTTCTGCCTC TTCAATCGTC TGCTTAGTGA CCCTGCTGCT 
GGAGCCTACA AAGAGCTAGT GACTTTTGCC AAATACATCC TGGGCAAATT TTTTGCACTG 
GCTGCAGTCA ACCAAAAAGC CTTTGTGGAG CTGTTGTTCT GGAAGAACAC AGCTGTGGTT 
CGAGAGATGA CTGAGGGCTA TGGCTCCCTG GATGACAGGT CTTCCAGTCG CAGAGCACCT 
ACATGGAGCC CCGAAGAAGA GGCTCATCTT CGGGAGCTGT ACCTCGCCAA TAAGGACGTG 
GAAGGGCAGG ATGTGGTGGA AGCCATCTTG GCCCACCTGA ATACTGTTCC TCGAACACGC 
AAGCAGATCA TCCACCATCT GGTACAGATG GGACTGGCTG ACAGTGTCAA GGACTTCCAA 
AGGAAAGGAA CCCATATTGT ACTGTGGACG GGGGATCAGG AGTTGGAGCT GCAGCGGCTT 
TTTGAGGAAT TCCGGGACTC AGATGATGTC CTGGGTCATA TCATGAAGAA TATCACAGCC 
AAACGCTCAC GGGCCCGAAT AGTGGATAAA CTCTTGGCTC TGGGGCTGGT GGCTGAGCGG 
CGGGAGCTGT ACAAGAAACG GCAGAAAAAG TTGGCATCCT CCATCTTGCC AAATGGAGCG 
GAGTCCCTGA AAGATTTTTG CCAGGAAGAT CTGGAAGAAG AGGAAAACCT GCCTGAGGAA 
GACAGCGAAG AGGAAGAAGA AGGGGGCTCA GAAGCAGAAC AAGTCCAGGG TAGCTTAGTC 
CTTTCAAATG AAAACCTTGG TCAAAGCCTG CATCAGGAAG GCTTTTCTAT CCCGCTCCTA 
TGGCTCCAGA ACTGCCTGAT CCGAGCAGCT GATGATCGGG AAGAGGATGG CTGCTCCCAG 
GCCGTTCCAT TGGTGCCACT CACAGAGGAA AATGAGGAAG CCATGGAAAA CGAACAGTTT 
CAGCAGCTGT TGCGCAAGCT AGGGGTTCGG CCCCCTGCCT CTGGGCAGGA AACCTTCTGG 
CGAATTCCAG CCAAGCTGAG TCCTACCCAG CTCCGGAGGG CAGCAGCTTC TTTGAGTCAA 
CCAGAGGAGG AACAGAAGCT GCAGCCAGAG CTGCAGCCTA AAGTCCCTGG AGAGCAAGGC 
TCTGATGAGG AGCACTGTAA AGAGCACCGA GCACAAGCCC TGAGGGCCCT CTTGCTAGCC 
CACAAGAAGA AAGCGGGCCT GGCATCCCCA GAGGAGGAAG ACGCTGTTGG TAAAGAGCCG 
CTGAAGGCAG CACCCAAGAA ACGACAATTG CTGGACAGCG ACGAGGAACA GGAAGAAGAT 
GAGGGCAGGA ACAGAGCACC AGAGTTGGGA GCTCCAGGAA TCCAAAAGAA GAAACGATAC 
CAGATTGAGG ATGATGAGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMELESS ( NM_001330295.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317224.1 Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 2, mRNA.

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CloneID	OHu62315
Clone ID Related Accession (Same CDS sequence)	NM_001330295.1 , NM_001330295.2
Accession Version	NM_001330295.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	protein timeless homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY040179.1, AF098162.1, BC039842.1, BC031514.1, AK000721.1 and BE503292.1. On Aug 23, 2016 this sequence version replaced XM_011538939.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) has the same N- and C-termini, but is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.165098.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTTGC ACATGATGAA CTGTGAACTT CTAGCCACAT GTAGTGCCCT TGGGTACTTG 
GAGGGAGACA CTTACCATAA GGAACCAGAT TGCTTAGAGA GCGTGAAGGA TCTGATCCGC 
TATTTGAGGC ATGAGGATGA GACACGAGAT GTGCGGCAGC AGCTGGGGGC AGCCCAGATC 
CTACAGAGCG ACCTTCTGCC CATCCTCACC CAGCACCACC AGGACAAGCC TCTCTTTGAT 
GCTGTTATCA GACTGATGGT GAACTTGACA CAACCAGCCT TGCTCTGTTT TGGCAATCTG 
CCTAAGGAGC CCAGCTTTCG GCACCATTTT TTGCAGGTGC TAACTTATTT GCAGGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCTTTT GGAGTCCTCA GTGAAACCTT GTATGAGCTG 
CTGCAGCTGG GCTGGGAGGA ACGGCAGGAG GAAGACAACT TGCTGATTGA ACGGATCCTA 
CTGCTGGTCA GAAATATTCT CCATGTCCCA GCTGACCTTG ATCAGGAGAA GATTGATGAT 
GACGCCAGTG CCCATGACCA GCTCCTCTGG GCGATTCACC TCAGCGGCCT GGATGACCTG 
CTCCTCTTTC TGGCCAGCTC GTCTGCTGAG GAGCAATGGA GCCTACATGT GCTAGAGATT 
GTCTCCCTTA TGTTTCGTGA CCAGAACCCC GAGCAGCTGG CGGGAGTAGG GCAGGGACGC 
TTAGCTCAGG AGCGGAGTGC AGATTTTGCA GAACTGGAGG TGTTGCGCCA GCGAGAGATG 
GCAGAAAAGA AGACTCGAGC CCTCCAGCGA GGCAACAGGC ATTCTCGATT TGGGGGCTCC 
TATATTGTCC AGGGGTTGAA ATCCATTGGG GAGAGGGACC TCATCTTTCA CAAAGGCCTT 
CACAACCTAC GAAACTACAG TTCAGATTTG GGAAAGCAGC CGAAAAAGGT GCCTAAACGT 
CGCCAGGCCG CCCGAGAGCT GTCCATTCAG CGCCGTTCTG CCCTCAATGT GAGGCTCTTC 
CTCAGAGACT TCTGCTCTGA GTTCCTGGAG AACTGTTACA ACCGGCTCAT GGGATCAGTA 
AAGGATCACC TGCTTCGGGA GAAAGCTCAG CAGCATGATG AGACCTATTA TATGTGGGCC 
TTGGCTTTCT TCATGGCCTT CAACCGAGCT GCCTCCTTCC GGCCAGGCCT GGTTTCTGAG 
ACCCTCAGTG TCCGTACCTT CCACTTCATT GAGCAGAACC TCACCAACTA CTATGAGATG 
ATGCTGACTG ACCGCAAGGA AGCTGCCTCC TGGGCACGCC GGATGCACTT GGCTCTGAAG 
GCCTATCAGG AGCTGCTGGC AACAGTGAAT GAGATGGACA TATCTCCAGA TGAGGCTGTG 
AGGGAGAGCA GCCGCATCAT CAAGAACAAT ATTTTCTATG TGATGGAGTA CCGAGAACTA 
TTCCTGGCAC TTTTTCGAAA GTTTGATGAG AGATGCCAGC CCCGCTCTTT CCTTCGTGAC 
CTGGTGGAGA CCACCCACCT CTTCCTCAAA ATGTTGGAGC GATTCTGTCG GAGCCGTGGG 
AACCTGGTGG TGCAGAACAA ACAAAAGAAG AGAAGGAAGA AGAAGAAGAA GGTCCTAGAC 
CAGGCCATTG TTTCTGGTAA TGTCCCATCT AGCCCAGAAG AAGTGGAGGC TGTGTGGCCA 
GCCCTGGCTG AGCAGCTACA GTGCTGTGCC CAGAATTCTG AGCTCAGCAT GGACTCCGTG 
GTTCCCTTTG ATGCGGCCTC AGAGGTGCCA GTGGAGGAGC AGCGGGCAGA AGCTATGGTA 
CGGATCCAAG ACTGTCTCCT GGCTGGCCAG GCCCCACAGG CCCTGACTCT CCTGAGGTCT 
GCTCGGGAGG TGTGGCCTGA AGGAGATGTG TTTGGCTCTC AAGACATTTC TCCAGAGGAA 
GAGATCCAGT TGCTGAAACA AATCCTCTCT GCTCCACTTC CCCGGCAGCA GGGCCCAGAG 
GAACGTGGGG CAGAGGAAGA AGAAGAGGAG GAGGAGGAGG AAGAGGAGGA GTTGCAAGTG 
GTCCAGGTGT CGGAGAAAGA ATTTAATTTT CTGGACTACC TGAAACGCTT TGCATGTTCA 
ACTGTCGTTC GAGCCTATGT GCTGCTACTA AGGAGCTACC AGCAGAATAG TGCCCACACT 
AACCATTGCA TTGTGAAGAT GCTGCACCGG CTGGCCCATG ACCTCAAAAT GGAAGCCCTA 
CTTTTTCAGC TGTCAGTCTT CTGCCTCTTC AATCGTCTGC TTAGTGACCC TGCTGCTGGA 
GCCTACAAAG AGCTAGTGAC TTTTGCCAAA TACATCCTGG GCAAATTTTT TGCACTGGCT 
GCAGTCAACC AAAAAGCCTT TGTGGAGCTG TTGTTCTGGA AGAACACAGC TGTGGTTCGA 
GAGATGACTG AGGGCTATGG CTCCCTGGAT GACAGGTCTT CCAGTCGCAG AGCACCTACA 
TGGAGCCCCG AAGAAGAGGC TCATCTTCGG GAGCTGTACC TCGCCAATAA GGACGTGGAA 
GGGCAGGATG TGGTGGAAGC CATCTTGGCC CACCTGAATA CTGTTCCTCG AACACGCAAG 
CAGATCATCC ACCATCTGGT ACAGATGGGA CTGGCTGACA GTGTCAAGGA CTTCCAAAGG 
AAAGGAACCC ATATTGTACT GTGGACGGGG GATCAGGAGT TGGAGCTGCA GCGGCTTTTT 
GAGGAATTCC GGGACTCAGA TGATGTCCTG GGTCATATCA TGAAGAATAT CACAGCCAAA 
CGCTCACGGG CCCGAATAGT GGATAAACTC TTGGCTCTGG GGCTGGTGGC TGAGCGGCGG 
GAGCTGTACA AGAAACGGCA GAAAAAGTTG GCATCCTCCA TCTTGCCAAA TGGAGCGGAG 
TCCCTGAAAG ATTTTTGCCA GGAAGATCTG GAAGAAGAGG AAAACCTGCC TGAGGAAGAC 
AGCGAAGAGG AAGAAGAAGG GGGCTCAGAA GCAGAACAAG TCCAGGGTAG CTTAGTCCTT 
TCAAATGAAA ACCTTGGTCA AAGCCTGCAT CAGGAAGGCT TTTCTATCCC GCTCCTATGG 
CTCCAGAACT GCCTGATCCG AGCAGCTGAT GATCGGGAAG AGGATGGCTG CTCCCAGGCC 
GTTCCATTGG TGCCACTCAC AGAGGAAAAT GAGGAAGCCA TGGAAAACGA ACAGTTTCAG 
CAGCTGTTGC GCAAGCTAGG GGTTCGGCCC CCTGCCTCTG GGCAGGAAAC CTTCTGGCGA 
ATTCCAGCCA AGCTGAGTCC TACCCAGCTC CGGAGGGCAG CAGCTTCTTT GAGTCAACCA 
GAGGAGGAAC AGAAGCTGCA GCCAGAGCTG CAGCCTAAAG TCCCTGGAGA GCAAGGCTCT 
GATGAGGAGC ACTGTAAAGA GCACCGAGCA CAAGCCCTGA GGGCCCTCTT GCTAGCCCAC 
AAGAAGAAAG CGGGCCTGGC ATCCCCAGAG GAGGAAGACG CTGTTGGTAA AGAGCCGCTG 
AAGGCAGCAC CCAAGAAACG ACAATTGCTG GACAGCGACG AGGAACAGGA AGAAGATGAG 
GGCAGGAACA GAGCACCAGA GTTGGGAGCT CCAGGAATCC AAAAGAAGAA ACGATACCAG 
ATTGAGGATG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317224.1
CDS	218..3841
Translation	MDLHMMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHHQDKPLFDAVIRLMVNLTQPALLCFGNLPKEPSFRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNILHVPADLDQEKIDDDASAHDQLLWAIHLSGLDDLLLFLASSSAEEQWSLHVLEIVSLMFRDQNPEQLAGVGQGRLAQERSADFAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIVQGLKSIGERDLIFHKGLHNLRNYSSDLGKQPKKVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREKAQQHDETYYMWALAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDISPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRSRGNLVVQNKQKKRRKKKKKVLDQAIVSGNVPSSPEEVEAVWPALAEQLQCCAQNSELSMDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALTLLRSAREVWPEGDVFGSQDISPEEEIQLLKQILSAPLPRQQGPEERGAEEEEEEEEEEEEELQVVQVSEKEFNFLDYLKRFACSTVVRAYVLLLRSYQQNSAHTNHCIVKMLHRLAHDLKMEALLFQLSVFCLFNRLLSDPAAGAYKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGSLDDRSSSRRAPTWSPEEEAHLRELYLANKDVEGQDVVEAILAHLNTVPRTRKQIIHHLVQMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEEDSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSIPLLWLQNCLIRAADDREEDGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQGSDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRYQIEDDEDD

Target ORF information:

RefSeq Version	NM_001330295.1
Organism	Homo sapiens(human)
Definition	Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330295.1

ORF Insert Sequence:

ATGGACTTGC ACATGATGAA CTGTGAACTT CTAGCCACAT GTAGTGCCCT TGGGTACTTG 
GAGGGAGACA CTTACCATAA GGAACCAGAT TGCTTAGAGA GCGTGAAGGA TCTGATCCGC 
TATTTGAGGC ATGAGGATGA GACACGAGAT GTGCGGCAGC AGCTGGGGGC AGCCCAGATC 
CTACAGAGCG ACCTTCTGCC CATCCTCACC CAGCACCACC AGGACAAGCC TCTCTTTGAT 
GCTGTTATCA GACTGATGGT GAACTTGACA CAACCAGCCT TGCTCTGTTT TGGCAATCTG 
CCTAAGGAGC CCAGCTTTCG GCACCATTTT TTGCAGGTGC TAACTTATTT GCAGGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCTTTT GGAGTCCTCA GTGAAACCTT GTATGAGCTG 
CTGCAGCTGG GCTGGGAGGA ACGGCAGGAG GAAGACAACT TGCTGATTGA ACGGATCCTA 
CTGCTGGTCA GAAATATTCT CCATGTCCCA GCTGACCTTG ATCAGGAGAA GATTGATGAT 
GACGCCAGTG CCCATGACCA GCTCCTCTGG GCGATTCACC TCAGCGGCCT GGATGACCTG 
CTCCTCTTTC TGGCCAGCTC GTCTGCTGAG GAGCAATGGA GCCTACATGT GCTAGAGATT 
GTCTCCCTTA TGTTTCGTGA CCAGAACCCC GAGCAGCTGG CGGGAGTAGG GCAGGGACGC 
TTAGCTCAGG AGCGGAGTGC AGATTTTGCA GAACTGGAGG TGTTGCGCCA GCGAGAGATG 
GCAGAAAAGA AGACTCGAGC CCTCCAGCGA GGCAACAGGC ATTCTCGATT TGGGGGCTCC 
TATATTGTCC AGGGGTTGAA ATCCATTGGG GAGAGGGACC TCATCTTTCA CAAAGGCCTT 
CACAACCTAC GAAACTACAG TTCAGATTTG GGAAAGCAGC CGAAAAAGGT GCCTAAACGT 
CGCCAGGCCG CCCGAGAGCT GTCCATTCAG CGCCGTTCTG CCCTCAATGT GAGGCTCTTC 
CTCAGAGACT TCTGCTCTGA GTTCCTGGAG AACTGTTACA ACCGGCTCAT GGGATCAGTA 
AAGGATCACC TGCTTCGGGA GAAAGCTCAG CAGCATGATG AGACCTATTA TATGTGGGCC 
TTGGCTTTCT TCATGGCCTT CAACCGAGCT GCCTCCTTCC GGCCAGGCCT GGTTTCTGAG 
ACCCTCAGTG TCCGTACCTT CCACTTCATT GAGCAGAACC TCACCAACTA CTATGAGATG 
ATGCTGACTG ACCGCAAGGA AGCTGCCTCC TGGGCACGCC GGATGCACTT GGCTCTGAAG 
GCCTATCAGG AGCTGCTGGC AACAGTGAAT GAGATGGACA TATCTCCAGA TGAGGCTGTG 
AGGGAGAGCA GCCGCATCAT CAAGAACAAT ATTTTCTATG TGATGGAGTA CCGAGAACTA 
TTCCTGGCAC TTTTTCGAAA GTTTGATGAG AGATGCCAGC CCCGCTCTTT CCTTCGTGAC 
CTGGTGGAGA CCACCCACCT CTTCCTCAAA ATGTTGGAGC GATTCTGTCG GAGCCGTGGG 
AACCTGGTGG TGCAGAACAA ACAAAAGAAG AGAAGGAAGA AGAAGAAGAA GGTCCTAGAC 
CAGGCCATTG TTTCTGGTAA TGTCCCATCT AGCCCAGAAG AAGTGGAGGC TGTGTGGCCA 
GCCCTGGCTG AGCAGCTACA GTGCTGTGCC CAGAATTCTG AGCTCAGCAT GGACTCCGTG 
GTTCCCTTTG ATGCGGCCTC AGAGGTGCCA GTGGAGGAGC AGCGGGCAGA AGCTATGGTA 
CGGATCCAAG ACTGTCTCCT GGCTGGCCAG GCCCCACAGG CCCTGACTCT CCTGAGGTCT 
GCTCGGGAGG TGTGGCCTGA AGGAGATGTG TTTGGCTCTC AAGACATTTC TCCAGAGGAA 
GAGATCCAGT TGCTGAAACA AATCCTCTCT GCTCCACTTC CCCGGCAGCA GGGCCCAGAG 
GAACGTGGGG CAGAGGAAGA AGAAGAGGAG GAGGAGGAGG AAGAGGAGGA GTTGCAAGTG 
GTCCAGGTGT CGGAGAAAGA ATTTAATTTT CTGGACTACC TGAAACGCTT TGCATGTTCA 
ACTGTCGTTC GAGCCTATGT GCTGCTACTA AGGAGCTACC AGCAGAATAG TGCCCACACT 
AACCATTGCA TTGTGAAGAT GCTGCACCGG CTGGCCCATG ACCTCAAAAT GGAAGCCCTA 
CTTTTTCAGC TGTCAGTCTT CTGCCTCTTC AATCGTCTGC TTAGTGACCC TGCTGCTGGA 
GCCTACAAAG AGCTAGTGAC TTTTGCCAAA TACATCCTGG GCAAATTTTT TGCACTGGCT 
GCAGTCAACC AAAAAGCCTT TGTGGAGCTG TTGTTCTGGA AGAACACAGC TGTGGTTCGA 
GAGATGACTG AGGGCTATGG CTCCCTGGAT GACAGGTCTT CCAGTCGCAG AGCACCTACA 
TGGAGCCCCG AAGAAGAGGC TCATCTTCGG GAGCTGTACC TCGCCAATAA GGACGTGGAA 
GGGCAGGATG TGGTGGAAGC CATCTTGGCC CACCTGAATA CTGTTCCTCG AACACGCAAG 
CAGATCATCC ACCATCTGGT ACAGATGGGA CTGGCTGACA GTGTCAAGGA CTTCCAAAGG 
AAAGGAACCC ATATTGTACT GTGGACGGGG GATCAGGAGT TGGAGCTGCA GCGGCTTTTT 
GAGGAATTCC GGGACTCAGA TGATGTCCTG GGTCATATCA TGAAGAATAT CACAGCCAAA 
CGCTCACGGG CCCGAATAGT GGATAAACTC TTGGCTCTGG GGCTGGTGGC TGAGCGGCGG 
GAGCTGTACA AGAAACGGCA GAAAAAGTTG GCATCCTCCA TCTTGCCAAA TGGAGCGGAG 
TCCCTGAAAG ATTTTTGCCA GGAAGATCTG GAAGAAGAGG AAAACCTGCC TGAGGAAGAC 
AGCGAAGAGG AAGAAGAAGG GGGCTCAGAA GCAGAACAAG TCCAGGGTAG CTTAGTCCTT 
TCAAATGAAA ACCTTGGTCA AAGCCTGCAT CAGGAAGGCT TTTCTATCCC GCTCCTATGG 
CTCCAGAACT GCCTGATCCG AGCAGCTGAT GATCGGGAAG AGGATGGCTG CTCCCAGGCC 
GTTCCATTGG TGCCACTCAC AGAGGAAAAT GAGGAAGCCA TGGAAAACGA ACAGTTTCAG 
CAGCTGTTGC GCAAGCTAGG GGTTCGGCCC CCTGCCTCTG GGCAGGAAAC CTTCTGGCGA 
ATTCCAGCCA AGCTGAGTCC TACCCAGCTC CGGAGGGCAG CAGCTTCTTT GAGTCAACCA 
GAGGAGGAAC AGAAGCTGCA GCCAGAGCTG CAGCCTAAAG TCCCTGGAGA GCAAGGCTCT 
GATGAGGAGC ACTGTAAAGA GCACCGAGCA CAAGCCCTGA GGGCCCTCTT GCTAGCCCAC 
AAGAAGAAAG CGGGCCTGGC ATCCCCAGAG GAGGAAGACG CTGTTGGTAA AGAGCCGCTG 
AAGGCAGCAC CCAAGAAACG ACAATTGCTG GACAGCGACG AGGAACAGGA AGAAGATGAG 
GGCAGGAACA GAGCACCAGA GTTGGGAGCT CCAGGAATCC AAAAGAAGAA ACGATACCAG 
ATTGAGGATG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMELESS ( NM_001330295.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317224.1 Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 2, mRNA.

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CloneID	OHu62315
Clone ID Related Accession (Same CDS sequence)	NM_001330295.1 , NM_001330295.2
Accession Version	NM_001330295.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	protein timeless homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC024884.31. On Jun 1, 2019 this sequence version replaced NM_001330295.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) has the same N- and C-termini, but is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.165098.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTTGC ACATGATGAA CTGTGAACTT CTAGCCACAT GTAGTGCCCT TGGGTACTTG 
GAGGGAGACA CTTACCATAA GGAACCAGAT TGCTTAGAGA GCGTGAAGGA TCTGATCCGC 
TATTTGAGGC ATGAGGATGA GACACGAGAT GTGCGGCAGC AGCTGGGGGC AGCCCAGATC 
CTACAGAGCG ACCTTCTGCC CATCCTCACC CAGCACCACC AGGACAAGCC TCTCTTTGAT 
GCTGTTATCA GACTGATGGT GAACTTGACA CAACCAGCCT TGCTCTGTTT TGGCAATCTG 
CCTAAGGAGC CCAGCTTTCG GCACCATTTT TTGCAGGTGC TAACTTATTT GCAGGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCTTTT GGAGTCCTCA GTGAAACCTT GTATGAGCTG 
CTGCAGCTGG GCTGGGAGGA ACGGCAGGAG GAAGACAACT TGCTGATTGA ACGGATCCTA 
CTGCTGGTCA GAAATATTCT CCATGTCCCA GCTGACCTTG ATCAGGAGAA GATTGATGAT 
GACGCCAGTG CCCATGACCA GCTCCTCTGG GCGATTCACC TCAGCGGCCT GGATGACCTG 
CTCCTCTTTC TGGCCAGCTC GTCTGCTGAG GAGCAATGGA GCCTACATGT GCTAGAGATT 
GTCTCCCTTA TGTTTCGTGA CCAGAACCCC GAGCAGCTGG CGGGAGTAGG GCAGGGACGC 
TTAGCTCAGG AGCGGAGTGC AGATTTTGCA GAACTGGAGG TGTTGCGCCA GCGAGAGATG 
GCAGAAAAGA AGACTCGAGC CCTCCAGCGA GGCAACAGGC ATTCTCGATT TGGGGGCTCC 
TATATTGTCC AGGGGTTGAA ATCCATTGGG GAGAGGGACC TCATCTTTCA CAAAGGCCTT 
CACAACCTAC GAAACTACAG TTCAGATTTG GGAAAGCAGC CGAAAAAGGT GCCTAAACGT 
CGCCAGGCCG CCCGAGAGCT GTCCATTCAG CGCCGTTCTG CCCTCAATGT GAGGCTCTTC 
CTCAGAGACT TCTGCTCTGA GTTCCTGGAG AACTGTTACA ACCGGCTCAT GGGATCAGTA 
AAGGATCACC TGCTTCGGGA GAAAGCTCAG CAGCATGATG AGACCTATTA TATGTGGGCC 
TTGGCTTTCT TCATGGCCTT CAACCGAGCT GCCTCCTTCC GGCCAGGCCT GGTTTCTGAG 
ACCCTCAGTG TCCGTACCTT CCACTTCATT GAGCAGAACC TCACCAACTA CTATGAGATG 
ATGCTGACTG ACCGCAAGGA AGCTGCCTCC TGGGCACGCC GGATGCACTT GGCTCTGAAG 
GCCTATCAGG AGCTGCTGGC AACAGTGAAT GAGATGGACA TATCTCCAGA TGAGGCTGTG 
AGGGAGAGCA GCCGCATCAT CAAGAACAAT ATTTTCTATG TGATGGAGTA CCGAGAACTA 
TTCCTGGCAC TTTTTCGAAA GTTTGATGAG AGATGCCAGC CCCGCTCTTT CCTTCGTGAC 
CTGGTGGAGA CCACCCACCT CTTCCTCAAA ATGTTGGAGC GATTCTGTCG GAGCCGTGGG 
AACCTGGTGG TGCAGAACAA ACAAAAGAAG AGAAGGAAGA AGAAGAAGAA GGTCCTAGAC 
CAGGCCATTG TTTCTGGTAA TGTCCCATCT AGCCCAGAAG AAGTGGAGGC TGTGTGGCCA 
GCCCTGGCTG AGCAGCTACA GTGCTGTGCC CAGAATTCTG AGCTCAGCAT GGACTCCGTG 
GTTCCCTTTG ATGCGGCCTC AGAGGTGCCA GTGGAGGAGC AGCGGGCAGA AGCTATGGTA 
CGGATCCAAG ACTGTCTCCT GGCTGGCCAG GCCCCACAGG CCCTGACTCT CCTGAGGTCT 
GCTCGGGAGG TGTGGCCTGA AGGAGATGTG TTTGGCTCTC AAGACATTTC TCCAGAGGAA 
GAGATCCAGT TGCTGAAACA AATCCTCTCT GCTCCACTTC CCCGGCAGCA GGGCCCAGAG 
GAACGTGGGG CAGAGGAAGA AGAAGAGGAG GAGGAGGAGG AAGAGGAGGA GTTGCAAGTG 
GTCCAGGTGT CGGAGAAAGA ATTTAATTTT CTGGACTACC TGAAACGCTT TGCATGTTCA 
ACTGTCGTTC GAGCCTATGT GCTGCTACTA AGGAGCTACC AGCAGAATAG TGCCCACACT 
AACCATTGCA TTGTGAAGAT GCTGCACCGG CTGGCCCATG ACCTCAAAAT GGAAGCCCTA 
CTTTTTCAGC TGTCAGTCTT CTGCCTCTTC AATCGTCTGC TTAGTGACCC TGCTGCTGGA 
GCCTACAAAG AGCTAGTGAC TTTTGCCAAA TACATCCTGG GCAAATTTTT TGCACTGGCT 
GCAGTCAACC AAAAAGCCTT TGTGGAGCTG TTGTTCTGGA AGAACACAGC TGTGGTTCGA 
GAGATGACTG AGGGCTATGG CTCCCTGGAT GACAGGTCTT CCAGTCGCAG AGCACCTACA 
TGGAGCCCCG AAGAAGAGGC TCATCTTCGG GAGCTGTACC TCGCCAATAA GGACGTGGAA 
GGGCAGGATG TGGTGGAAGC CATCTTGGCC CACCTGAATA CTGTTCCTCG AACACGCAAG 
CAGATCATCC ACCATCTGGT ACAGATGGGA CTGGCTGACA GTGTCAAGGA CTTCCAAAGG 
AAAGGAACCC ATATTGTACT GTGGACGGGG GATCAGGAGT TGGAGCTGCA GCGGCTTTTT 
GAGGAATTCC GGGACTCAGA TGATGTCCTG GGTCATATCA TGAAGAATAT CACAGCCAAA 
CGCTCACGGG CCCGAATAGT GGATAAACTC TTGGCTCTGG GGCTGGTGGC TGAGCGGCGG 
GAGCTGTACA AGAAACGGCA GAAAAAGTTG GCATCCTCCA TCTTGCCAAA TGGAGCGGAG 
TCCCTGAAAG ATTTTTGCCA GGAAGATCTG GAAGAAGAGG AAAACCTGCC TGAGGAAGAC 
AGCGAAGAGG AAGAAGAAGG GGGCTCAGAA GCAGAACAAG TCCAGGGTAG CTTAGTCCTT 
TCAAATGAAA ACCTTGGTCA AAGCCTGCAT CAGGAAGGCT TTTCTATCCC GCTCCTATGG 
CTCCAGAACT GCCTGATCCG AGCAGCTGAT GATCGGGAAG AGGATGGCTG CTCCCAGGCC 
GTTCCATTGG TGCCACTCAC AGAGGAAAAT GAGGAAGCCA TGGAAAACGA ACAGTTTCAG 
CAGCTGTTGC GCAAGCTAGG GGTTCGGCCC CCTGCCTCTG GGCAGGAAAC CTTCTGGCGA 
ATTCCAGCCA AGCTGAGTCC TACCCAGCTC CGGAGGGCAG CAGCTTCTTT GAGTCAACCA 
GAGGAGGAAC AGAAGCTGCA GCCAGAGCTG CAGCCTAAAG TCCCTGGAGA GCAAGGCTCT 
GATGAGGAGC ACTGTAAAGA GCACCGAGCA CAAGCCCTGA GGGCCCTCTT GCTAGCCCAC 
AAGAAGAAAG CGGGCCTGGC ATCCCCAGAG GAGGAAGACG CTGTTGGTAA AGAGCCGCTG 
AAGGCAGCAC CCAAGAAACG ACAATTGCTG GACAGCGACG AGGAACAGGA AGAAGATGAG 
GGCAGGAACA GAGCACCAGA GTTGGGAGCT CCAGGAATCC AAAAGAAGAA ACGATACCAG 
ATTGAGGATG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317224.1
CDS	179..3802
Translation	MDLHMMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHHQDKPLFDAVIRLMVNLTQPALLCFGNLPKEPSFRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNILHVPADLDQEKIDDDASAHDQLLWAIHLSGLDDLLLFLASSSAEEQWSLHVLEIVSLMFRDQNPEQLAGVGQGRLAQERSADFAELEVLRQREMAEKKTRALQRGNRHSRFGGSYIVQGLKSIGERDLIFHKGLHNLRNYSSDLGKQPKKVPKRRQAARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREKAQQHDETYYMWALAFFMAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDISPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFCRSRGNLVVQNKQKKRRKKKKKVLDQAIVSGNVPSSPEEVEAVWPALAEQLQCCAQNSELSMDSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALTLLRSAREVWPEGDVFGSQDISPEEEIQLLKQILSAPLPRQQGPEERGAEEEEEEEEEEEEELQVVQVSEKEFNFLDYLKRFACSTVVRAYVLLLRSYQQNSAHTNHCIVKMLHRLAHDLKMEALLFQLSVFCLFNRLLSDPAAGAYKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGSLDDRSSSRRAPTWSPEEEAHLRELYLANKDVEGQDVVEAILAHLNTVPRTRKQIIHHLVQMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEEDSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSIPLLWLQNCLIRAADDREEDGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQGSDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRYQIEDDEDD

Target ORF information:

RefSeq Version	NM_001330295.2
Organism	Homo sapiens(human)
Definition	Homo sapiens timeless circadian regulator (TIMELESS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330295.2

ORF Insert Sequence:

ATGGACTTGC ACATGATGAA CTGTGAACTT CTAGCCACAT GTAGTGCCCT TGGGTACTTG 
GAGGGAGACA CTTACCATAA GGAACCAGAT TGCTTAGAGA GCGTGAAGGA TCTGATCCGC 
TATTTGAGGC ATGAGGATGA GACACGAGAT GTGCGGCAGC AGCTGGGGGC AGCCCAGATC 
CTACAGAGCG ACCTTCTGCC CATCCTCACC CAGCACCACC AGGACAAGCC TCTCTTTGAT 
GCTGTTATCA GACTGATGGT GAACTTGACA CAACCAGCCT TGCTCTGTTT TGGCAATCTG 
CCTAAGGAGC CCAGCTTTCG GCACCATTTT TTGCAGGTGC TAACTTATTT GCAGGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCTTTT GGAGTCCTCA GTGAAACCTT GTATGAGCTG 
CTGCAGCTGG GCTGGGAGGA ACGGCAGGAG GAAGACAACT TGCTGATTGA ACGGATCCTA 
CTGCTGGTCA GAAATATTCT CCATGTCCCA GCTGACCTTG ATCAGGAGAA GATTGATGAT 
GACGCCAGTG CCCATGACCA GCTCCTCTGG GCGATTCACC TCAGCGGCCT GGATGACCTG 
CTCCTCTTTC TGGCCAGCTC GTCTGCTGAG GAGCAATGGA GCCTACATGT GCTAGAGATT 
GTCTCCCTTA TGTTTCGTGA CCAGAACCCC GAGCAGCTGG CGGGAGTAGG GCAGGGACGC 
TTAGCTCAGG AGCGGAGTGC AGATTTTGCA GAACTGGAGG TGTTGCGCCA GCGAGAGATG 
GCAGAAAAGA AGACTCGAGC CCTCCAGCGA GGCAACAGGC ATTCTCGATT TGGGGGCTCC 
TATATTGTCC AGGGGTTGAA ATCCATTGGG GAGAGGGACC TCATCTTTCA CAAAGGCCTT 
CACAACCTAC GAAACTACAG TTCAGATTTG GGAAAGCAGC CGAAAAAGGT GCCTAAACGT 
CGCCAGGCCG CCCGAGAGCT GTCCATTCAG CGCCGTTCTG CCCTCAATGT GAGGCTCTTC 
CTCAGAGACT TCTGCTCTGA GTTCCTGGAG AACTGTTACA ACCGGCTCAT GGGATCAGTA 
AAGGATCACC TGCTTCGGGA GAAAGCTCAG CAGCATGATG AGACCTATTA TATGTGGGCC 
TTGGCTTTCT TCATGGCCTT CAACCGAGCT GCCTCCTTCC GGCCAGGCCT GGTTTCTGAG 
ACCCTCAGTG TCCGTACCTT CCACTTCATT GAGCAGAACC TCACCAACTA CTATGAGATG 
ATGCTGACTG ACCGCAAGGA AGCTGCCTCC TGGGCACGCC GGATGCACTT GGCTCTGAAG 
GCCTATCAGG AGCTGCTGGC AACAGTGAAT GAGATGGACA TATCTCCAGA TGAGGCTGTG 
AGGGAGAGCA GCCGCATCAT CAAGAACAAT ATTTTCTATG TGATGGAGTA CCGAGAACTA 
TTCCTGGCAC TTTTTCGAAA GTTTGATGAG AGATGCCAGC CCCGCTCTTT CCTTCGTGAC 
CTGGTGGAGA CCACCCACCT CTTCCTCAAA ATGTTGGAGC GATTCTGTCG GAGCCGTGGG 
AACCTGGTGG TGCAGAACAA ACAAAAGAAG AGAAGGAAGA AGAAGAAGAA GGTCCTAGAC 
CAGGCCATTG TTTCTGGTAA TGTCCCATCT AGCCCAGAAG AAGTGGAGGC TGTGTGGCCA 
GCCCTGGCTG AGCAGCTACA GTGCTGTGCC CAGAATTCTG AGCTCAGCAT GGACTCCGTG 
GTTCCCTTTG ATGCGGCCTC AGAGGTGCCA GTGGAGGAGC AGCGGGCAGA AGCTATGGTA 
CGGATCCAAG ACTGTCTCCT GGCTGGCCAG GCCCCACAGG CCCTGACTCT CCTGAGGTCT 
GCTCGGGAGG TGTGGCCTGA AGGAGATGTG TTTGGCTCTC AAGACATTTC TCCAGAGGAA 
GAGATCCAGT TGCTGAAACA AATCCTCTCT GCTCCACTTC CCCGGCAGCA GGGCCCAGAG 
GAACGTGGGG CAGAGGAAGA AGAAGAGGAG GAGGAGGAGG AAGAGGAGGA GTTGCAAGTG 
GTCCAGGTGT CGGAGAAAGA ATTTAATTTT CTGGACTACC TGAAACGCTT TGCATGTTCA 
ACTGTCGTTC GAGCCTATGT GCTGCTACTA AGGAGCTACC AGCAGAATAG TGCCCACACT 
AACCATTGCA TTGTGAAGAT GCTGCACCGG CTGGCCCATG ACCTCAAAAT GGAAGCCCTA 
CTTTTTCAGC TGTCAGTCTT CTGCCTCTTC AATCGTCTGC TTAGTGACCC TGCTGCTGGA 
GCCTACAAAG AGCTAGTGAC TTTTGCCAAA TACATCCTGG GCAAATTTTT TGCACTGGCT 
GCAGTCAACC AAAAAGCCTT TGTGGAGCTG TTGTTCTGGA AGAACACAGC TGTGGTTCGA 
GAGATGACTG AGGGCTATGG CTCCCTGGAT GACAGGTCTT CCAGTCGCAG AGCACCTACA 
TGGAGCCCCG AAGAAGAGGC TCATCTTCGG GAGCTGTACC TCGCCAATAA GGACGTGGAA 
GGGCAGGATG TGGTGGAAGC CATCTTGGCC CACCTGAATA CTGTTCCTCG AACACGCAAG 
CAGATCATCC ACCATCTGGT ACAGATGGGA CTGGCTGACA GTGTCAAGGA CTTCCAAAGG 
AAAGGAACCC ATATTGTACT GTGGACGGGG GATCAGGAGT TGGAGCTGCA GCGGCTTTTT 
GAGGAATTCC GGGACTCAGA TGATGTCCTG GGTCATATCA TGAAGAATAT CACAGCCAAA 
CGCTCACGGG CCCGAATAGT GGATAAACTC TTGGCTCTGG GGCTGGTGGC TGAGCGGCGG 
GAGCTGTACA AGAAACGGCA GAAAAAGTTG GCATCCTCCA TCTTGCCAAA TGGAGCGGAG 
TCCCTGAAAG ATTTTTGCCA GGAAGATCTG GAAGAAGAGG AAAACCTGCC TGAGGAAGAC 
AGCGAAGAGG AAGAAGAAGG GGGCTCAGAA GCAGAACAAG TCCAGGGTAG CTTAGTCCTT 
TCAAATGAAA ACCTTGGTCA AAGCCTGCAT CAGGAAGGCT TTTCTATCCC GCTCCTATGG 
CTCCAGAACT GCCTGATCCG AGCAGCTGAT GATCGGGAAG AGGATGGCTG CTCCCAGGCC 
GTTCCATTGG TGCCACTCAC AGAGGAAAAT GAGGAAGCCA TGGAAAACGA ACAGTTTCAG 
CAGCTGTTGC GCAAGCTAGG GGTTCGGCCC CCTGCCTCTG GGCAGGAAAC CTTCTGGCGA 
ATTCCAGCCA AGCTGAGTCC TACCCAGCTC CGGAGGGCAG CAGCTTCTTT GAGTCAACCA 
GAGGAGGAAC AGAAGCTGCA GCCAGAGCTG CAGCCTAAAG TCCCTGGAGA GCAAGGCTCT 
GATGAGGAGC ACTGTAAAGA GCACCGAGCA CAAGCCCTGA GGGCCCTCTT GCTAGCCCAC 
AAGAAGAAAG CGGGCCTGGC ATCCCCAGAG GAGGAAGACG CTGTTGGTAA AGAGCCGCTG 
AAGGCAGCAC CCAAGAAACG ACAATTGCTG GACAGCGACG AGGAACAGGA AGAAGATGAG 
GGCAGGAACA GAGCACCAGA GTTGGGAGCT CCAGGAATCC AAAAGAAGAA ACGATACCAG 
ATTGAGGATG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TIMELESS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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