EIF2S2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF2S2
Entrez Gene ID	8894
Full Name	eukaryotic translation initiation factor 2 subunit beta
Synonyms	EIF2,EIF2B,EIF2beta,PPP1R67,eIF-2-beta
General protein information	Preferred Names eukaryotic translation initiation factor 2 subunit beta Names eukaryotic translation initiation factor 2 subunit 2 eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa protein phosphatase 1, regulatory subunit 67
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q11.22
Summary	Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015].
Disorder MIM:	603908

mRNA and Protein(s)

mRNA	Protein	Name
NM_003908.3	NP_003899.2	eukaryotic translation initiation factor 2 subunit 2
NM_001316364.1	NP_001303293.1	eukaryotic translation initiation factor 2 subunit 2 isoform 3
NM_001316363.1	NP_001303292.1	eukaryotic translation initiation factor 2 subunit 2 isoform 2
NM_003908.5	NP_003899.2	eukaryotic translation initiation factor 2 subunit 2 isoform 1
NM_003908.4	NP_003899.2	eukaryotic translation initiation factor 2 subunit 2 isoform 1
NM_001316364.2	NP_001303293.1	eukaryotic translation initiation factor 2 subunit 2 isoform 3
NM_001316363.2	NP_001303292.1	eukaryotic translation initiation factor 2 subunit 2 isoform 2
XM_017028118.1	XP_016883607.1	eukaryotic translation initiation factor 2 subunit 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Eif2s2	NP_955412.1
Canis lupus familiaris (dog)	EIF2S2	XP_863416.2
Danio rerio (zebrafish)	eif2s2	NP_997840.1
Homo sapiens (human)	EIF2S2	NP_003899.2
	EIF2S2	XP_001105021.2
Bos taurus (cattle)	EIF2S2	NP_001015621.1
Gallus gallus (chicken)	EIF2S2	NP_989928.1
Mus musculus (house mouse)	Eif2s2	NP_080306.1
Drosophila melanogaster (fruit fly)	eIF-2beta	NP_524043.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007218	XP_308567.4
Pan troglodytes (chimpanzee)	EIF2S2	NP_001092026.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Association of eukaryotic translation initiation factor eIF2B with fully solubilized CXCR4.
Palmesino E, Apuzzo T, Thelen S, Mueller B, Langen H, Thelen M
Journal of leukocyte biology99(6)971-8(2016 06)

Essential role of eIF5-mimic protein in animal development is linked to control of ATF4 expression.
Hiraishi H, Oatman J, Haller SL, Blunk L, McGivern B, Morris J, Papadopoulos E, Gutierrez W, Gordon M, Bokhari W, Ikeda Y, Miles D, Fellers J, Asano M, Wagner G, Tazi L, Rothenburg S, Brown SJ, Asano K
Nucleic acids research42(16)10321-30(2014)

eIF2? mutation that disrupts eIF2 complex integrity links intellectual disability to impaired translation initiation.
Borck G, Shin BS, Stiller B, Mimouni-Bloch A, Thiele H, Kim JR, Thakur M, Skinner C, Aschenbach L, Smirin-Yosef P, Har-Zahav A, N?rnberg G, Altm?ller J, Frommolt P, Hofmann K, Konen O, N?rnberg P, Munnich A, Schwartz CE, Gothelf D, Colleaux L, Dever TE, Kubisch C, Basel-Vanagaite L
Molecular cell48(4)641-6(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mass spectrometry of CXCR4 immunoprecipitates revealed a specific interaction with the pentameric eukaryotic translation initiation factor 2B.
Title: Association of eukaryotic translation initiation factor eIF2B with fully solubilized CXCR4.

This study provides mechanistic insight into the role of eIF5-carboxyl terminal domain's dynamic interplay with eIF1 and eIF2beta
Title: The C-terminal domain of eukaryotic initiation factor 5 promotes start codon recognition by its dynamic interplay with eIF1 and eIF2?.

These findings suggest that alpha and beta subunits of eIF2 interact with each other and the beta-subunit plays a critical role both in the regulation and function of eIF2.
Title: Intersubunit and interprotein interactions of alpha- and beta-subunits of human eIF2: Effect of phosphorylation.

The following EIF2S2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2S2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03614	NM_003908.5 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu53150	NM_001316364.2 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu93152	XM_017028118.1 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu101437	NM_001316363.2 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu03614	NM_003908.3	Homo sapiens eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu03614	NM_003908.4	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu53150	NM_001316364.1	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu101437	NM_001316363.1	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF2S2 ( NM_003908.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003899.2 Homo sapiens eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), mRNA.

Price & Availability↑

CloneID	OHu03614
Clone ID Related Accession (Same CDS sequence)	NM_003908.3 , NM_003908.5 , NM_003908.4
Accession Version	NM_003908.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	eukaryotic translation initiation factor 2 subunit 2
Comment

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTATTACAT CGTCAGCCCA AACATCTCCT TGCATTTTTG 
TTGGCTGAAT TGGGTACAAG TGGTTCTATA GATGGTAATA ACCAACTTGT AATCAAAGGA 
AGATTCCAAC AGAAACAGAT AGAAAATGTC TTGAGAAGAT ATATCAAGGA ATATGTCACT 
TGTCACACAT GCCGATCACC GGACACAATC CTGCAGAAGG ACACACGACT CTATTTCCTA 
CAGTGCGAAA CTTGTCATTC TAGATGTTCT GTTGCCAGTA TCAAAACCGG CTTCCAGGCT 
GTCACGGGCA AGCGAGCACA GCTCCGTGCC AAAGCTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003899.2
Translation	MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Target ORF information:

RefSeq Version	NM_003908.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003908.3

ORF Insert Sequence:

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTATTACAT CGTCAGCCCA AACATCTCCT TGCATTTTTG 
TTGGCTGAAT TGGGTACAAG TGGTTCTATA GATGGTAATA ACCAACTTGT AATCAAAGGA 
AGATTCCAAC AGAAACAGAT AGAAAATGTC TTGAGAAGAT ATATCAAGGA ATATGTCACT 
TGTCACACAT GCCGATCACC GGACACAATC CTGCAGAAGG ACACACGACT CTATTTCCTA 
CAGTGCGAAA CTTGTCATTC TAGATGTTCT GTTGCCAGTA TCAAAACCGG CTTCCAGGCT 
GTCACGGGCA AGCGAGCACA GCTCCGTGCC AAAGCTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2S2 ( NM_003908.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003899.2 Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03614
Clone ID Related Accession (Same CDS sequence)	NM_003908.3 , NM_003908.5 , NM_003908.4
Accession Version	NM_003908.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2 subunit 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BC000461.2, AL031668.23 and CA436754.1. On Nov 22, 2018 this sequence version replaced NM_003908.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.96523.1, SRR1660803.162664.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374980.3/ ENSP00000364119.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTATTACAT CGTCAGCCCA AACATCTCCT TGCATTTTTG 
TTGGCTGAAT TGGGTACAAG TGGTTCTATA GATGGTAATA ACCAACTTGT AATCAAAGGA 
AGATTCCAAC AGAAACAGAT AGAAAATGTC TTGAGAAGAT ATATCAAGGA ATATGTCACT 
TGTCACACAT GCCGATCACC GGACACAATC CTGCAGAAGG ACACACGACT CTATTTCCTA 
CAGTGCGAAA CTTGTCATTC TAGATGTTCT GTTGCCAGTA TCAAAACCGG CTTCCAGGCT 
GTCACGGGCA AGCGAGCACA GCTCCGTGCC AAAGCTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003899.2
CDS	134..1135
Translation	MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Target ORF information:

RefSeq Version	NM_003908.5
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003908.5

ORF Insert Sequence:

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTATTACAT CGTCAGCCCA AACATCTCCT TGCATTTTTG 
TTGGCTGAAT TGGGTACAAG TGGTTCTATA GATGGTAATA ACCAACTTGT AATCAAAGGA 
AGATTCCAAC AGAAACAGAT AGAAAATGTC TTGAGAAGAT ATATCAAGGA ATATGTCACT 
TGTCACACAT GCCGATCACC GGACACAATC CTGCAGAAGG ACACACGACT CTATTTCCTA 
CAGTGCGAAA CTTGTCATTC TAGATGTTCT GTTGCCAGTA TCAAAACCGG CTTCCAGGCT 
GTCACGGGCA AGCGAGCACA GCTCCGTGCC AAAGCTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2S2 ( NM_003908.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003899.2 Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03614
Clone ID Related Accession (Same CDS sequence)	NM_003908.3 , NM_003908.5 , NM_003908.4
Accession Version	NM_003908.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2 subunit 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from HY005227.1, BC000461.2, AL031668.23 and CA436754.1. On Oct 17, 2015 this sequence version replaced NM_003908.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.96523.1, SRR1660803.162664.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTATTACAT CGTCAGCCCA AACATCTCCT TGCATTTTTG 
TTGGCTGAAT TGGGTACAAG TGGTTCTATA GATGGTAATA ACCAACTTGT AATCAAAGGA 
AGATTCCAAC AGAAACAGAT AGAAAATGTC TTGAGAAGAT ATATCAAGGA ATATGTCACT 
TGTCACACAT GCCGATCACC GGACACAATC CTGCAGAAGG ACACACGACT CTATTTCCTA 
CAGTGCGAAA CTTGTCATTC TAGATGTTCT GTTGCCAGTA TCAAAACCGG CTTCCAGGCT 
GTCACGGGCA AGCGAGCACA GCTCCGTGCC AAAGCTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003899.2
CDS	247..1248
Translation	MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Target ORF information:

RefSeq Version	NM_003908.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003908.4

ORF Insert Sequence:

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTATTACAT CGTCAGCCCA AACATCTCCT TGCATTTTTG 
TTGGCTGAAT TGGGTACAAG TGGTTCTATA GATGGTAATA ACCAACTTGT AATCAAAGGA 
AGATTCCAAC AGAAACAGAT AGAAAATGTC TTGAGAAGAT ATATCAAGGA ATATGTCACT 
TGTCACACAT GCCGATCACC GGACACAATC CTGCAGAAGG ACACACGACT CTATTTCCTA 
CAGTGCGAAA CTTGTCATTC TAGATGTTCT GTTGCCAGTA TCAAAACCGG CTTCCAGGCT 
GTCACGGGCA AGCGAGCACA GCTCCGTGCC AAAGCTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2S2 ( NM_001316364.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303293.1 Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu53150
Clone ID Related Accession (Same CDS sequence)	NM_001316364.1 , NM_001316364.2
Accession Version	NM_001316364.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2 subunit 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY005227.1, BX418731.2, AL031668.23 and CA436754.1. On Oct 17, 2015 this sequence version replaced XM_005260605.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' end compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.137730.1, SRR1803614.140779.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTTGGTTCT ATAGATGGTA ATAACCAACT TGTAATCAAA 
GGAAGATTCC AACAGAAACA GATAGAAAAT GTCTTGAGAA GATATATCAA GGAATATGTC 
ACTTGTCACA CATGCCGATC ACCGGACACA ATCCTGCAGA AGGACACACG ACTCTATTTC 
CTACAGTGCG AAACTTGTCA TTCTAGATGT TCTGTTGCCA GTATCAAAAC CGGCTTCCAG 
GCTGTCACGG GCAAGCGAGC ACAGCTCCGT GCCAAAGCTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303293.1
CDS	247..1191
Translation	MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Target ORF information:

RefSeq Version	NM_001316364.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316364.1

ORF Insert Sequence:

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTTGGTTCT ATAGATGGTA ATAACCAACT TGTAATCAAA 
GGAAGATTCC AACAGAAACA GATAGAAAAT GTCTTGAGAA GATATATCAA GGAATATGTC 
ACTTGTCACA CATGCCGATC ACCGGACACA ATCCTGCAGA AGGACACACG ACTCTATTTC 
CTACAGTGCG AAACTTGTCA TTCTAGATGT TCTGTTGCCA GTATCAAAAC CGGCTTCCAG 
GCTGTCACGG GCAAGCGAGC ACAGCTCCGT GCCAAAGCTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2S2 ( NM_001316364.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303293.1 Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu53150
Clone ID Related Accession (Same CDS sequence)	NM_001316364.1 , NM_001316364.2
Accession Version	NM_001316364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2 subunit 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY005227.1, BX418731.2, AL031668.23 and CA436754.1. On May 31, 2019 this sequence version replaced NM_001316364.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' end compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.137730.1, SRR7410570.210062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTTGGTTCT ATAGATGGTA ATAACCAACT TGTAATCAAA 
GGAAGATTCC AACAGAAACA GATAGAAAAT GTCTTGAGAA GATATATCAA GGAATATGTC 
ACTTGTCACA CATGCCGATC ACCGGACACA ATCCTGCAGA AGGACACACG ACTCTATTTC 
CTACAGTGCG AAACTTGTCA TTCTAGATGT TCTGTTGCCA GTATCAAAAC CGGCTTCCAG 
GCTGTCACGG GCAAGCGAGC ACAGCTCCGT GCCAAAGCTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303293.1
CDS	134..1078
Translation	MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Target ORF information:

RefSeq Version	NM_001316364.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316364.2

ORF Insert Sequence:

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGGAT 
CTTAAGATTG AAAGTGATGT TCAAGAACCA ACTGAACCAG AGGATGACCT TGACATTATG 
CTTGGCAATA AAAAGAAGAA AAAGAAGAAT GTTAAGTTCC CAGATGAGGA TGAAATACTA 
GAGAAAGATG AAGCTCTAGA AGATGAAGAC AACAAAAAAG ATGATGGTAT CTCATTCAGT 
AATCAGACAG GCCCTGCTTG GGCAGGCTCA GAAAGAGACT ACACATACGA GGAGCTGCTG 
AATCGAGTGT TCAACATCAT GAGGGAAAAG AATCCAGATA TGGTTGCTGG GGAGAAAAGG 
AAATTTGTCA TGAAACCTCC ACAAGTCGTC CGAGTAGGAA CCAAGAAAAC TTCTTTTGTC 
AACTTTACAG ATATCTGTAA ACTTGGTTCT ATAGATGGTA ATAACCAACT TGTAATCAAA 
GGAAGATTCC AACAGAAACA GATAGAAAAT GTCTTGAGAA GATATATCAA GGAATATGTC 
ACTTGTCACA CATGCCGATC ACCGGACACA ATCCTGCAGA AGGACACACG ACTCTATTTC 
CTACAGTGCG AAACTTGTCA TTCTAGATGT TCTGTTGCCA GTATCAAAAC CGGCTTCCAG 
GCTGTCACGG GCAAGCGAGC ACAGCTCCGT GCCAAAGCTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2S2 ( XM_017028118.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883607.1 Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu93152
Clone ID Related Accession (Same CDS sequence)	XM_017028118.1
Accession Version	XM_017028118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2 subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTTG ATCCTACTAT GAGCAAGAAG AAAAAGAAGA AGAAGAAGCC TTTTATGTTA 
GATGAGGAAG GGGATACCCA AACAGAGGAA ACCCAGCCTT CAGAAACAAA AGAAGTGGAG 
CCAGAGCCAA CTGAGGACAA GGATTTGGAA GCTGATGAAG AGGACACTAG GAAAAAAGAT 
GCTTCTGATG ATCTAGATGA CTTGAACTTC TTTAATCAAA AGAAAAAGAA GAAAAAAACT 
AAAAAGATAT TTGATATTGA TGAAGCTGAA GAAGGTGTAA AGGATCTTAA GATTGAAAGT 
GATGTTCAAG AACCAACTGA ACCAGAGGAT GACCTTGACA TTATGCTTGG CAATAAAAAG 
AAGAAAAAGA AGAATGTTAA GTTCCCAGAT GAGGATGAAA TACTAGAGAA AGATGAAGCT 
CTAGAAGATG AAGACAACAA AAAAGATGAT GGTATCTCAT TCAGTAATCA GACAGGCCCT 
GCTTGGGCAG GCTCAGAAAG AGACTACACA TACGAGGAGC TGCTGAATCG AGTGTTCAAC 
ATCATGAGGG AAAAGAATCC AGATATGGTT GCTGGGGAGA AAAGGAAATT TGTCATGAAA 
CCTCCACAAG TCGTCCGAGT AGGAACCAAG AAAACTTCTT TTGTCAACTT TACAGATATC 
TGTAAACTAT TACATCGTCA GCCCAAACAT CTCCTTGCAT TTTTGTTGGC TGAATTGGGT 
ACAAGTGGTT CTATAGATGG TAATAACCAA CTTGTAATCA AAGGAAGATT CCAACAGAAA 
CAGATAGAAA ATGTCTTGAG AAGATATATC AAGGAATATG TCACTTGTCA CACATGCCGA 
TCACCGGACA CAATCCTGCA GAAGGACACA CGACTCTATT TCCTACAGTG CGAAACTTGT 
CATTCTAGAT GTTCTGTTGC CAGTATCAAA ACCGGCTTCC AGGCTGTCAC GGGCAAGCGA 
GCACAGCTCC GTGCCAAAGC TAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883607.1
CDS	424..1410
Translation	MIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Target ORF information:

RefSeq Version	XM_017028118.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028118.1

ORF Insert Sequence:

ATGATTTTTG ATCCTACTAT GAGCAAGAAG AAAAAGAAGA AGAAGAAGCC TTTTATGTTA 
GATGAGGAAG GGGATACCCA AACAGAGGAA ACCCAGCCTT CAGAAACAAA AGAAGTGGAG 
CCAGAGCCAA CTGAGGACAA GGATTTGGAA GCTGATGAAG AGGACACTAG GAAAAAAGAT 
GCTTCTGATG ATCTAGATGA CTTGAACTTC TTTAATCAAA AGAAAAAGAA GAAAAAAACT 
AAAAAGATAT TTGATATTGA TGAAGCTGAA GAAGGTGTAA AGGATCTTAA GATTGAAAGT 
GATGTTCAAG AACCAACTGA ACCAGAGGAT GACCTTGACA TTATGCTTGG CAATAAAAAG 
AAGAAAAAGA AGAATGTTAA GTTCCCAGAT GAGGATGAAA TACTAGAGAA AGATGAAGCT 
CTAGAAGATG AAGACAACAA AAAAGATGAT GGTATCTCAT TCAGTAATCA GACAGGCCCT 
GCTTGGGCAG GCTCAGAAAG AGACTACACA TACGAGGAGC TGCTGAATCG AGTGTTCAAC 
ATCATGAGGG AAAAGAATCC AGATATGGTT GCTGGGGAGA AAAGGAAATT TGTCATGAAA 
CCTCCACAAG TCGTCCGAGT AGGAACCAAG AAAACTTCTT TTGTCAACTT TACAGATATC 
TGTAAACTAT TACATCGTCA GCCCAAACAT CTCCTTGCAT TTTTGTTGGC TGAATTGGGT 
ACAAGTGGTT CTATAGATGG TAATAACCAA CTTGTAATCA AAGGAAGATT CCAACAGAAA 
CAGATAGAAA ATGTCTTGAG AAGATATATC AAGGAATATG TCACTTGTCA CACATGCCGA 
TCACCGGACA CAATCCTGCA GAAGGACACA CGACTCTATT TCCTACAGTG CGAAACTTGT 
CATTCTAGAT GTTCTGTTGC CAGTATCAAA ACCGGCTTCC AGGCTGTCAC GGGCAAGCGA 
GCACAGCTCC GTGCCAAAGC TAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2S2 ( NM_001316363.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303292.1 Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101437
Clone ID Related Accession (Same CDS sequence)	NM_001316363.1 , NM_001316363.2
Accession Version	NM_001316363.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2 subunit 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY005227.1, BX429051.2, AL031668.23 and CA436754.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.328020.1, BX443349.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGATT 
GAAAGTGATG TTCAAGAACC AACTGAACCA GAGGATGACC TTGACATTAT GCTTGGCAAT 
AAAAAGAAGA AAAAGAAGAA TGTTAAGTTC CCAGATGAGG ATGAAATACT AGAGAAAGAT 
GAAGCTCTAG AAGATGAAGA CAACAAAAAA GATGATGGTA TCTCATTCAG TAATCAGACA 
GGCCCTGCTT GGGCAGGCTC AGAAAGAGAC TACACATACG AGGAGCTGCT GAATCGAGTG 
TTCAACATCA TGAGGGAAAA GAATCCAGAT ATGGTTGCTG GGGAGAAAAG GAAATTTGTC 
ATGAAACCTC CACAAGTCGT CCGAGTAGGA ACCAAGAAAA CTTCTTTTGT CAACTTTACA 
GATATCTGTA AACTATTACA TCGTCAGCCC AAACATCTCC TTGCATTTTT GTTGGCTGAA 
TTGGGTACAA GTGGTTCTAT AGATGGTAAT AACCAACTTG TAATCAAAGG AAGATTCCAA 
CAGAAACAGA TAGAAAATGT CTTGAGAAGA TATATCAAGG AATATGTCAC TTGTCACACA 
TGCCGATCAC CGGACACAAT CCTGCAGAAG GACACACGAC TCTATTTCCT ACAGTGCGAA 
ACTTGTCATT CTAGATGTTC TGTTGCCAGT ATCAAAACCG GCTTCCAGGC TGTCACGGGC 
AAGCGAGCAC AGCTCCGTGC CAAAGCTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303292.1
CDS	247..1239
Translation	MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Target ORF information:

RefSeq Version	NM_001316363.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316363.1

ORF Insert Sequence:

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGATT 
GAAAGTGATG TTCAAGAACC AACTGAACCA GAGGATGACC TTGACATTAT GCTTGGCAAT 
AAAAAGAAGA AAAAGAAGAA TGTTAAGTTC CCAGATGAGG ATGAAATACT AGAGAAAGAT 
GAAGCTCTAG AAGATGAAGA CAACAAAAAA GATGATGGTA TCTCATTCAG TAATCAGACA 
GGCCCTGCTT GGGCAGGCTC AGAAAGAGAC TACACATACG AGGAGCTGCT GAATCGAGTG 
TTCAACATCA TGAGGGAAAA GAATCCAGAT ATGGTTGCTG GGGAGAAAAG GAAATTTGTC 
ATGAAACCTC CACAAGTCGT CCGAGTAGGA ACCAAGAAAA CTTCTTTTGT CAACTTTACA 
GATATCTGTA AACTATTACA TCGTCAGCCC AAACATCTCC TTGCATTTTT GTTGGCTGAA 
TTGGGTACAA GTGGTTCTAT AGATGGTAAT AACCAACTTG TAATCAAAGG AAGATTCCAA 
CAGAAACAGA TAGAAAATGT CTTGAGAAGA TATATCAAGG AATATGTCAC TTGTCACACA 
TGCCGATCAC CGGACACAAT CCTGCAGAAG GACACACGAC TCTATTTCCT ACAGTGCGAA 
ACTTGTCATT CTAGATGTTC TGTTGCCAGT ATCAAAACCG GCTTCCAGGC TGTCACGGGC 
AAGCGAGCAC AGCTCCGTGC CAAAGCTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2S2 ( NM_001316363.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303292.1 Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101437
Clone ID Related Accession (Same CDS sequence)	NM_001316363.1 , NM_001316363.2
Accession Version	NM_001316363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2 subunit 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY005227.1, BX429051.2, AL031668.23 and CA436754.1. On May 31, 2019 this sequence version replaced NM_001316363.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.328020.1, BX429051.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGATT 
GAAAGTGATG TTCAAGAACC AACTGAACCA GAGGATGACC TTGACATTAT GCTTGGCAAT 
AAAAAGAAGA AAAAGAAGAA TGTTAAGTTC CCAGATGAGG ATGAAATACT AGAGAAAGAT 
GAAGCTCTAG AAGATGAAGA CAACAAAAAA GATGATGGTA TCTCATTCAG TAATCAGACA 
GGCCCTGCTT GGGCAGGCTC AGAAAGAGAC TACACATACG AGGAGCTGCT GAATCGAGTG 
TTCAACATCA TGAGGGAAAA GAATCCAGAT ATGGTTGCTG GGGAGAAAAG GAAATTTGTC 
ATGAAACCTC CACAAGTCGT CCGAGTAGGA ACCAAGAAAA CTTCTTTTGT CAACTTTACA 
GATATCTGTA AACTATTACA TCGTCAGCCC AAACATCTCC TTGCATTTTT GTTGGCTGAA 
TTGGGTACAA GTGGTTCTAT AGATGGTAAT AACCAACTTG TAATCAAAGG AAGATTCCAA 
CAGAAACAGA TAGAAAATGT CTTGAGAAGA TATATCAAGG AATATGTCAC TTGTCACACA 
TGCCGATCAC CGGACACAAT CCTGCAGAAG GACACACGAC TCTATTTCCT ACAGTGCGAA 
ACTTGTCATT CTAGATGTTC TGTTGCCAGT ATCAAAACCG GCTTCCAGGC TGTCACGGGC 
AAGCGAGCAC AGCTCCGTGC CAAAGCTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303292.1
CDS	134..1126
Translation	MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

Target ORF information:

RefSeq Version	NM_001316363.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 subunit beta (EIF2S2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316363.2

ORF Insert Sequence:

ATGTCTGGGG ACGAGATGAT TTTTGATCCT ACTATGAGCA AGAAGAAAAA GAAGAAGAAG 
AAGCCTTTTA TGTTAGATGA GGAAGGGGAT ACCCAAACAG AGGAAACCCA GCCTTCAGAA 
ACAAAAGAAG TGGAGCCAGA GCCAACTGAG GACAAGGATT TGGAAGCTGA TGAAGAGGAC 
ACTAGGAAAA AAGATGCTTC TGATGATCTA GATGACTTGA ACTTCTTTAA TCAAAAGAAA 
AAGAAGAAAA AAACTAAAAA GATATTTGAT ATTGATGAAG CTGAAGAAGG TGTAAAGATT 
GAAAGTGATG TTCAAGAACC AACTGAACCA GAGGATGACC TTGACATTAT GCTTGGCAAT 
AAAAAGAAGA AAAAGAAGAA TGTTAAGTTC CCAGATGAGG ATGAAATACT AGAGAAAGAT 
GAAGCTCTAG AAGATGAAGA CAACAAAAAA GATGATGGTA TCTCATTCAG TAATCAGACA 
GGCCCTGCTT GGGCAGGCTC AGAAAGAGAC TACACATACG AGGAGCTGCT GAATCGAGTG 
TTCAACATCA TGAGGGAAAA GAATCCAGAT ATGGTTGCTG GGGAGAAAAG GAAATTTGTC 
ATGAAACCTC CACAAGTCGT CCGAGTAGGA ACCAAGAAAA CTTCTTTTGT CAACTTTACA 
GATATCTGTA AACTATTACA TCGTCAGCCC AAACATCTCC TTGCATTTTT GTTGGCTGAA 
TTGGGTACAA GTGGTTCTAT AGATGGTAAT AACCAACTTG TAATCAAAGG AAGATTCCAA 
CAGAAACAGA TAGAAAATGT CTTGAGAAGA TATATCAAGG AATATGTCAC TTGTCACACA 
TGCCGATCAC CGGACACAAT CCTGCAGAAG GACACACGAC TCTATTTCCT ACAGTGCGAA 
ACTTGTCATT CTAGATGTTC TGTTGCCAGT ATCAAAACCG GCTTCCAGGC TGTCACGGGC 
AAGCGAGCAC AGCTCCGTGC CAAAGCTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Association of eukaryotic translation initiation factor eIF2B with fully solubilized CXCR4.
Journal of leukocyte biology99(6)971-8(2016 06)
Palmesino E,Apuzzo T,Thelen S,Mueller B,Langen H,Thelen M

Essential role of eIF5-mimic protein in animal development is linked to control of ATF4 expression.
Nucleic acids research42(16)10321-30(2014)
Hiraishi H,Oatman J,Haller SL,Blunk L,McGivern B,Morris J,Papadopoulos E,Gutierrez W,Gordon M,Bokhari W,Ikeda Y,Miles D,Fellers J,Asano M,Wagner G,Tazi L,Rothenburg S,Brown SJ,Asano K

eIF2? mutation that disrupts eIF2 complex integrity links intellectual disability to impaired translation initiation.
Molecular cell48(4)641-6(2012 Nov)
Borck G,Shin BS,Stiller B,Mimouni-Bloch A,Thiele H,Kim JR,Thakur M,Skinner C,Aschenbach L,Smirin-Yosef P,Har-Zahav A,N?rnberg G,Altm?ller J,Frommolt P,Hofmann K,Konen O,N?rnberg P,Munnich A,Schwartz CE,Gothelf D,Colleaux L,Dever TE,Kubisch C,Basel-Vanagaite L

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EIF2S2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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