DDX18 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX18
Entrez Gene ID	8886
Full Name	DEAD-box helicase 18
Synonyms	Has1,MrDb
General protein information	Preferred Names DEAD-box helicase 18 Names ATP-dependent RNA helicase DDX18 DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 DEAD box protein 18 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 18 (Myc-regulated) Myc-regulated DEAD box protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q14.1
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq, Jul 2008].
Disorder MIM:	606355

mRNA and Protein(s)

mRNA	Protein	Name
NM_006773.3	NP_006764.3	ATP-dependent RNA helicase DDX18
NM_006773.4	NP_006764.3	ATP-dependent RNA helicase DDX18

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX18	NP_006764.3
Bos taurus (cattle)	DDX18	XP_002685472.1
Danio rerio (zebrafish)	ddx18	NP_001003411.1
Drosophila melanogaster (fruit fly)	pit	NP_732694.2
Eremothecium gossypii	AGOS_AGR333C	NP_986999.2
Arabidopsis thaliana (thale cress)	AT3G18600	NP_188490.1
Pan troglodytes (chimpanzee)	DDX18	XP_515753.2
Canis lupus familiaris (dog)	DDX18	XP_533327.3
Mus musculus (house mouse)	Ddx18	NP_080136.2
Caenorhabditis elegans (roundworm)	B0511.6	NP_492779.1
Saccharomyces cerevisiae (baker's yeast)	HAS1	NP_014017.1
Neurospora crassa	NCU09349	XP_958928.1
Oryza sativa (rice)	Os06g0535100	NP_001057788.2
Xenopus tropicalis (tropical clawed frog)	ddx18	NP_001016776.1
Rattus norvegicus (Norway rat)	Ddx18	NP_001006997.1
Schizosaccharomyces pombe (fission yeast)	has1	NP_594488.1
Gallus gallus (chicken)	DDX18	XP_422125.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008601	XP_314700.4
Kluyveromyces lactis	KLLA0A09669g	XP_451422.1
Magnaporthe oryzae (rice blast fungus)	MGG_06522	XP_003717029.1
Arabidopsis thaliana (thale cress)	AT5G65900	NP_201391.1

Gene Ontology
Bibliography

Related articles in PubMed

Genomic interaction between ER and HMGB2 identifies DDX18 as a novel driver of endocrine resistance in breast cancer cells.
Redmond AM, Byrne C, Bane FT, Brown GD, Tibbitts P, O'Brien K, Hill AD, Carroll JS, Young LS
Oncogene34(29)3871-80(2015 Jul)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Ddx18 is essential for cell-cycle progression in zebrafish hematopoietic cells and is mutated in human AML.
Payne EM, Bolli N, Rhodes J, Abdel-Wahab OI, Levine R, Hedvat CV, Stone R, Khanna-Gupta A, Sun H, Kanki JP, Gazda HT, Beggs AH, Cotter FE, Look AT
Blood118(4)903-15(2011 Jul)

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Ohta S, Bukowski-Wills JC, Sanchez-Pulido L, Alves Fde L, Wood L, Chen ZA, Platani M, Fischer L, Hudson DF, Ponting CP, Fukagawa T, Earnshaw WC, Rappsilber J
Cell142(5)810-21(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that odulation of RNA helicase DDX18 directly affects growth of tamoxifen-resistant cells, suggesting that it may be a critical downstream effector of the estrogen receptors (ERs) and high mobility group box 2 (HMGB2) complex.
Title: Genomic interaction between ER and HMGB2 identifies DDX18 as a novel driver of endocrine resistance in breast cancer cells.

We identified 4 nonsynonymous sequence variants of DDX18 in acute myeloid leukemia patient samples
Title: Ddx18 is essential for cell-cycle progression in zebrafish hematopoietic cells and is mutated in human AML.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variation and antioxidant response gene expression in the bronchial airway epithelium of smokers at risk for lung cancer.

findings suggest that MrDb is important for cell proliferation and that its inhibition could prevent tumor cell proliferation.
Title: Cellular studies of MrDb (DDX18).

The following DDX18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30823	NM_006773.4 Latest version!	Homo sapiens DEAD-box helicase 18 (DDX18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu30823	NM_006773.3	Homo sapiens DEAD-box helicase 18 (DDX18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX18 ( NM_006773.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006764.3 Homo sapiens DEAD-box helicase 18 (DDX18), mRNA.

Price & Availability↑

CloneID	OHu30823
Clone ID Related Accession (Same CDS sequence)	NM_006773.3 , NM_006773.4
Accession Version	NM_006773.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU599536.1, BC024739.1 and AK091227.1. On Nov 14, 2003 this sequence version replaced NM_006773.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024739.1, SRR1660809.78754.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCACACC TGCCGATGAA ACTCCTGCGT AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGGCAGCGGA ACCTAAAGTT TCAGGGGGCC TCAAATCTGA CCCTATCGGA AACTCAAAAT 
GGAGATGTAT CTGAAGAAAC AATGGGAAGT AGAAAGGTTA AAAAATCAAA ACAAAAGCCC 
ATGAATGTGG GCTTATCAGA AACTCAAAAT GGAGGCATGT CTCAAGAAGC AGTGGGAAAT 
ATAAAAGTTA CAAAGTCTCC CCAGAAATCC ACTGTATTAA CCAATGGAGA AGCAGCAATG 
CAGTCTTCCA ATTCAGAATC AAAAAAGAAA AAGAAGAAAA AGAGAAAAAT GGTGAATGAT 
GCTGAGCCTG ATACGAAAAA AGCAAAAACT GAAAACAAAG GGAAATCTGA AGAAGAAAGT 
GCCGAGACTA CTAAAGAAAC AGAAAATAAT GTGGAGAAGC CAGATAATGA TGAAGATGAG 
AGTGAGGTGC CCAGTCTGCC CCTGGGACTG ACAGGAGCTT TTGAGGATAC TTCGTTTGCT 
TCTCTATGTA ATCTTGTCAA TGAAAACACT CTGAAGGCAA TAAAAGAAAT GGGTTTTACA 
AACATGACTG AAATTCAGCA TAAAAGTATC AGACCACTTC TGGAAGGCAG GGATCTTCTA 
GCAGCTGCAA AAACAGGCAG TGGTAAAACC CTGGCTTTTC TCATCCCTGC AGTTGAACTC 
ATTGTTAAGT TAAGGTTCAT GCCCAGGAAT GGAACAGGAG TCCTTATTCT CTCACCTACT 
AGAGAACTAG CCATGCAAAC CTTTGGTGTT CTTAAGGAGC TGATGACTCA CCACGTGCAT 
ACCTATGGCT TGATAATGGG TGGCAGTAAC AGATCTGCTG AAGCACAGAA ACTTGGTAAT 
GGGATCAACA TCATTGTGGC CACACCAGGC CGTCTGCTGG ACCATATGCA GAATACCCCA 
GGATTTATGT ATAAAAACCT GCAGTGTCTG GTTATTGATG AAGCTGATCG TATCTTGGAT 
GTGGGGTTTG AAGAGGAATT AAAGCAAATT ATTAAACTTT TGCCAACACG TAGACAGACT 
ATGCTCTTTT CTGCCACCCA AACTCGAAAA GTTGAAGACC TGGCAAGGAT TTCTCTGAAA 
AAGGAGCCAT TGTATGTTGG CGTTGATGAT GATAAAGCGA ATGCAACAGT GGATGGTCTT 
GAACAGGGAT ATGTTGTTTG TCCTTCTGAA AAGAGATTCC TTCTGCTCTT TACATTCCTT 
AAGAAGAACC GAAAGAAGAA GCTTATGGTC TTCTTTTCAT CTTGTATGTC TGTGAAATAC 
CACTATGAGT TGCTGAACTA CATTGATTTG CCCGTCTTGG CCATTCATGG AAAGCAAAAG 
CAAAATAAGC GTACAACCAC ATTCTTCCAG TTCTGCAATG CAGATTCGGG AACACTATTG 
TGTACGGATG TGGCAGCGAG AGGACTAGAC ATTCCTGAAG TCGACTGGAT TGTTCAGTAT 
GACCCTCCGG ATGACCCTAA GGAATATATT CATCGTGTGG GTAGAACAGC CAGAGGCCTA 
AATGGGAGAG GGCATGCCTT GCTCATTTTG CGCCCAGAAG AATTGGGTTT TCTTCGCTAC 
TTGAAACAAT CCAAGGTTCC ATTAAGTGAA TTTGACTTTT CCTGGTCTAA AATTTCTGAC 
ATTCAGTCTC AGCTTGAGAA ATTGATTGAA AAGAATTACT TTCTTCATAA GTCAGCCCAG 
GAAGCATATA AGTCATACAT ACGAGCCTAT GATTCCCATT CTCTGAAACA GATCTTTAAT 
GTTAATAACC TAAATTTGCC TCAGGTTGCT CTGTCATTTG GTTTCAAGGT GCCTCCCTTC 
GTTGATCTGA ACGTCAACAG TAATGAAGGC AAGCAGAAAA AGCGAGGAGG TGGTGGTGGA 
TTTGGCTACC AGAAAACCAA GAAAGTTGAG AAATCCAAAA TCTTTAAACA CATTAGCAAG 
AAATCATCTG ACAGCAGGCA GTTCTCTCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006764.3
CDS	100..2112
Translation	MSHLPMKLLRKKIEKRNLKLRQRNLKFQGASNLTLSETQNGDVSEETMGSRKVKKSKQKPMNVGLSETQNGGMSQEAVGNIKVTKSPQKSTVLTNGEAAMQSSNSESKKKKKKKRKMVNDAEPDTKKAKTENKGKSEEESAETTKETENNVEKPDNDEDESEVPSLPLGLTGAFEDTSFASLCNLVNENTLKAIKEMGFTNMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPAVELIVKLRFMPRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEAQKLGNGINIIVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPTRRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKANATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCMSVKYHYELLNYIDLPVLAIHGKQKQNKRTTTFFQFCNADSGTLLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLSEFDFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNSNEGKQKKRGGGGGFGYQKTKKVEKSKIFKHISKKSSDSRQFSH

Target ORF information:

RefSeq Version	NM_006773.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 18 (DDX18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006773.3

ORF Insert Sequence:

ATGTCACACC TGCCGATGAA ACTCCTGCGT AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGGCAGCGGA ACCTAAAGTT TCAGGGGGCC TCAAATCTGA CCCTATCGGA AACTCAAAAT 
GGAGATGTAT CTGAAGAAAC AATGGGAAGT AGAAAGGTTA AAAAATCAAA ACAAAAGCCC 
ATGAATGTGG GCTTATCAGA AACTCAAAAT GGAGGCATGT CTCAAGAAGC AGTGGGAAAT 
ATAAAAGTTA CAAAGTCTCC CCAGAAATCC ACTGTATTAA CCAATGGAGA AGCAGCAATG 
CAGTCTTCCA ATTCAGAATC AAAAAAGAAA AAGAAGAAAA AGAGAAAAAT GGTGAATGAT 
GCTGAGCCTG ATACGAAAAA AGCAAAAACT GAAAACAAAG GGAAATCTGA AGAAGAAAGT 
GCCGAGACTA CTAAAGAAAC AGAAAATAAT GTGGAGAAGC CAGATAATGA TGAAGATGAG 
AGTGAGGTGC CCAGTCTGCC CCTGGGACTG ACAGGAGCTT TTGAGGATAC TTCGTTTGCT 
TCTCTATGTA ATCTTGTCAA TGAAAACACT CTGAAGGCAA TAAAAGAAAT GGGTTTTACA 
AACATGACTG AAATTCAGCA TAAAAGTATC AGACCACTTC TGGAAGGCAG GGATCTTCTA 
GCAGCTGCAA AAACAGGCAG TGGTAAAACC CTGGCTTTTC TCATCCCTGC AGTTGAACTC 
ATTGTTAAGT TAAGGTTCAT GCCCAGGAAT GGAACAGGAG TCCTTATTCT CTCACCTACT 
AGAGAACTAG CCATGCAAAC CTTTGGTGTT CTTAAGGAGC TGATGACTCA CCACGTGCAT 
ACCTATGGCT TGATAATGGG TGGCAGTAAC AGATCTGCTG AAGCACAGAA ACTTGGTAAT 
GGGATCAACA TCATTGTGGC CACACCAGGC CGTCTGCTGG ACCATATGCA GAATACCCCA 
GGATTTATGT ATAAAAACCT GCAGTGTCTG GTTATTGATG AAGCTGATCG TATCTTGGAT 
GTGGGGTTTG AAGAGGAATT AAAGCAAATT ATTAAACTTT TGCCAACACG TAGACAGACT 
ATGCTCTTTT CTGCCACCCA AACTCGAAAA GTTGAAGACC TGGCAAGGAT TTCTCTGAAA 
AAGGAGCCAT TGTATGTTGG CGTTGATGAT GATAAAGCGA ATGCAACAGT GGATGGTCTT 
GAACAGGGAT ATGTTGTTTG TCCTTCTGAA AAGAGATTCC TTCTGCTCTT TACATTCCTT 
AAGAAGAACC GAAAGAAGAA GCTTATGGTC TTCTTTTCAT CTTGTATGTC TGTGAAATAC 
CACTATGAGT TGCTGAACTA CATTGATTTG CCCGTCTTGG CCATTCATGG AAAGCAAAAG 
CAAAATAAGC GTACAACCAC ATTCTTCCAG TTCTGCAATG CAGATTCGGG AACACTATTG 
TGTACGGATG TGGCAGCGAG AGGACTAGAC ATTCCTGAAG TCGACTGGAT TGTTCAGTAT 
GACCCTCCGG ATGACCCTAA GGAATATATT CATCGTGTGG GTAGAACAGC CAGAGGCCTA 
AATGGGAGAG GGCATGCCTT GCTCATTTTG CGCCCAGAAG AATTGGGTTT TCTTCGCTAC 
TTGAAACAAT CCAAGGTTCC ATTAAGTGAA TTTGACTTTT CCTGGTCTAA AATTTCTGAC 
ATTCAGTCTC AGCTTGAGAA ATTGATTGAA AAGAATTACT TTCTTCATAA GTCAGCCCAG 
GAAGCATATA AGTCATACAT ACGAGCCTAT GATTCCCATT CTCTGAAACA GATCTTTAAT 
GTTAATAACC TAAATTTGCC TCAGGTTGCT CTGTCATTTG GTTTCAAGGT GCCTCCCTTC 
GTTGATCTGA ACGTCAACAG TAATGAAGGC AAGCAGAAAA AGCGAGGAGG TGGTGGTGGA 
TTTGGCTACC AGAAAACCAA GAAAGTTGAG AAATCCAAAA TCTTTAAACA CATTAGCAAG 
AAATCATCTG ACAGCAGGCA GTTCTCTCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX18 ( NM_006773.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006764.3 Homo sapiens DEAD-box helicase 18 (DDX18), mRNA.

Price & Availability↑

CloneID	OHu30823
Clone ID Related Accession (Same CDS sequence)	NM_006773.3 , NM_006773.4
Accession Version	NM_006773.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU599536.1, BC024739.1 and AK091227.1. On Nov 22, 2018 this sequence version replaced NM_006773.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024739.1, SRR1803613.252788.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263239.7/ ENSP00000263239.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCACACC TGCCGATGAA ACTCCTGCGT AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGGCAGCGGA ACCTAAAGTT TCAGGGGGCC TCAAATCTGA CCCTATCGGA AACTCAAAAT 
GGAGATGTAT CTGAAGAAAC AATGGGAAGT AGAAAGGTTA AAAAATCAAA ACAAAAGCCC 
ATGAATGTGG GCTTATCAGA AACTCAAAAT GGAGGCATGT CTCAAGAAGC AGTGGGAAAT 
ATAAAAGTTA CAAAGTCTCC CCAGAAATCC ACTGTATTAA CCAATGGAGA AGCAGCAATG 
CAGTCTTCCA ATTCAGAATC AAAAAAGAAA AAGAAGAAAA AGAGAAAAAT GGTGAATGAT 
GCTGAGCCTG ATACGAAAAA AGCAAAAACT GAAAACAAAG GGAAATCTGA AGAAGAAAGT 
GCCGAGACTA CTAAAGAAAC AGAAAATAAT GTGGAGAAGC CAGATAATGA TGAAGATGAG 
AGTGAGGTGC CCAGTCTGCC CCTGGGACTG ACAGGAGCTT TTGAGGATAC TTCGTTTGCT 
TCTCTATGTA ATCTTGTCAA TGAAAACACT CTGAAGGCAA TAAAAGAAAT GGGTTTTACA 
AACATGACTG AAATTCAGCA TAAAAGTATC AGACCACTTC TGGAAGGCAG GGATCTTCTA 
GCAGCTGCAA AAACAGGCAG TGGTAAAACC CTGGCTTTTC TCATCCCTGC AGTTGAACTC 
ATTGTTAAGT TAAGGTTCAT GCCCAGGAAT GGAACAGGAG TCCTTATTCT CTCACCTACT 
AGAGAACTAG CCATGCAAAC CTTTGGTGTT CTTAAGGAGC TGATGACTCA CCACGTGCAT 
ACCTATGGCT TGATAATGGG TGGCAGTAAC AGATCTGCTG AAGCACAGAA ACTTGGTAAT 
GGGATCAACA TCATTGTGGC CACACCAGGC CGTCTGCTGG ACCATATGCA GAATACCCCA 
GGATTTATGT ATAAAAACCT GCAGTGTCTG GTTATTGATG AAGCTGATCG TATCTTGGAT 
GTGGGGTTTG AAGAGGAATT AAAGCAAATT ATTAAACTTT TGCCAACACG TAGACAGACT 
ATGCTCTTTT CTGCCACCCA AACTCGAAAA GTTGAAGACC TGGCAAGGAT TTCTCTGAAA 
AAGGAGCCAT TGTATGTTGG CGTTGATGAT GATAAAGCGA ATGCAACAGT GGATGGTCTT 
GAACAGGGAT ATGTTGTTTG TCCTTCTGAA AAGAGATTCC TTCTGCTCTT TACATTCCTT 
AAGAAGAACC GAAAGAAGAA GCTTATGGTC TTCTTTTCAT CTTGTATGTC TGTGAAATAC 
CACTATGAGT TGCTGAACTA CATTGATTTG CCCGTCTTGG CCATTCATGG AAAGCAAAAG 
CAAAATAAGC GTACAACCAC ATTCTTCCAG TTCTGCAATG CAGATTCGGG AACACTATTG 
TGTACGGATG TGGCAGCGAG AGGACTAGAC ATTCCTGAAG TCGACTGGAT TGTTCAGTAT 
GACCCTCCGG ATGACCCTAA GGAATATATT CATCGTGTGG GTAGAACAGC CAGAGGCCTA 
AATGGGAGAG GGCATGCCTT GCTCATTTTG CGCCCAGAAG AATTGGGTTT TCTTCGCTAC 
TTGAAACAAT CCAAGGTTCC ATTAAGTGAA TTTGACTTTT CCTGGTCTAA AATTTCTGAC 
ATTCAGTCTC AGCTTGAGAA ATTGATTGAA AAGAATTACT TTCTTCATAA GTCAGCCCAG 
GAAGCATATA AGTCATACAT ACGAGCCTAT GATTCCCATT CTCTGAAACA GATCTTTAAT 
GTTAATAACC TAAATTTGCC TCAGGTTGCT CTGTCATTTG GTTTCAAGGT GCCTCCCTTC 
GTTGATCTGA ACGTCAACAG TAATGAAGGC AAGCAGAAAA AGCGAGGAGG TGGTGGTGGA 
TTTGGCTACC AGAAAACCAA GAAAGTTGAG AAATCCAAAA TCTTTAAACA CATTAGCAAG 
AAATCATCTG ACAGCAGGCA GTTCTCTCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006764.3
CDS	88..2100
Translation	MSHLPMKLLRKKIEKRNLKLRQRNLKFQGASNLTLSETQNGDVSEETMGSRKVKKSKQKPMNVGLSETQNGGMSQEAVGNIKVTKSPQKSTVLTNGEAAMQSSNSESKKKKKKKRKMVNDAEPDTKKAKTENKGKSEEESAETTKETENNVEKPDNDEDESEVPSLPLGLTGAFEDTSFASLCNLVNENTLKAIKEMGFTNMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPAVELIVKLRFMPRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEAQKLGNGINIIVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPTRRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKANATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCMSVKYHYELLNYIDLPVLAIHGKQKQNKRTTTFFQFCNADSGTLLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLSEFDFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNSNEGKQKKRGGGGGFGYQKTKKVEKSKIFKHISKKSSDSRQFSH

Target ORF information:

RefSeq Version	NM_006773.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 18 (DDX18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006773.4

ORF Insert Sequence:

ATGTCACACC TGCCGATGAA ACTCCTGCGT AAGAAGATCG AGAAGCGGAA CCTCAAATTG 
CGGCAGCGGA ACCTAAAGTT TCAGGGGGCC TCAAATCTGA CCCTATCGGA AACTCAAAAT 
GGAGATGTAT CTGAAGAAAC AATGGGAAGT AGAAAGGTTA AAAAATCAAA ACAAAAGCCC 
ATGAATGTGG GCTTATCAGA AACTCAAAAT GGAGGCATGT CTCAAGAAGC AGTGGGAAAT 
ATAAAAGTTA CAAAGTCTCC CCAGAAATCC ACTGTATTAA CCAATGGAGA AGCAGCAATG 
CAGTCTTCCA ATTCAGAATC AAAAAAGAAA AAGAAGAAAA AGAGAAAAAT GGTGAATGAT 
GCTGAGCCTG ATACGAAAAA AGCAAAAACT GAAAACAAAG GGAAATCTGA AGAAGAAAGT 
GCCGAGACTA CTAAAGAAAC AGAAAATAAT GTGGAGAAGC CAGATAATGA TGAAGATGAG 
AGTGAGGTGC CCAGTCTGCC CCTGGGACTG ACAGGAGCTT TTGAGGATAC TTCGTTTGCT 
TCTCTATGTA ATCTTGTCAA TGAAAACACT CTGAAGGCAA TAAAAGAAAT GGGTTTTACA 
AACATGACTG AAATTCAGCA TAAAAGTATC AGACCACTTC TGGAAGGCAG GGATCTTCTA 
GCAGCTGCAA AAACAGGCAG TGGTAAAACC CTGGCTTTTC TCATCCCTGC AGTTGAACTC 
ATTGTTAAGT TAAGGTTCAT GCCCAGGAAT GGAACAGGAG TCCTTATTCT CTCACCTACT 
AGAGAACTAG CCATGCAAAC CTTTGGTGTT CTTAAGGAGC TGATGACTCA CCACGTGCAT 
ACCTATGGCT TGATAATGGG TGGCAGTAAC AGATCTGCTG AAGCACAGAA ACTTGGTAAT 
GGGATCAACA TCATTGTGGC CACACCAGGC CGTCTGCTGG ACCATATGCA GAATACCCCA 
GGATTTATGT ATAAAAACCT GCAGTGTCTG GTTATTGATG AAGCTGATCG TATCTTGGAT 
GTGGGGTTTG AAGAGGAATT AAAGCAAATT ATTAAACTTT TGCCAACACG TAGACAGACT 
ATGCTCTTTT CTGCCACCCA AACTCGAAAA GTTGAAGACC TGGCAAGGAT TTCTCTGAAA 
AAGGAGCCAT TGTATGTTGG CGTTGATGAT GATAAAGCGA ATGCAACAGT GGATGGTCTT 
GAACAGGGAT ATGTTGTTTG TCCTTCTGAA AAGAGATTCC TTCTGCTCTT TACATTCCTT 
AAGAAGAACC GAAAGAAGAA GCTTATGGTC TTCTTTTCAT CTTGTATGTC TGTGAAATAC 
CACTATGAGT TGCTGAACTA CATTGATTTG CCCGTCTTGG CCATTCATGG AAAGCAAAAG 
CAAAATAAGC GTACAACCAC ATTCTTCCAG TTCTGCAATG CAGATTCGGG AACACTATTG 
TGTACGGATG TGGCAGCGAG AGGACTAGAC ATTCCTGAAG TCGACTGGAT TGTTCAGTAT 
GACCCTCCGG ATGACCCTAA GGAATATATT CATCGTGTGG GTAGAACAGC CAGAGGCCTA 
AATGGGAGAG GGCATGCCTT GCTCATTTTG CGCCCAGAAG AATTGGGTTT TCTTCGCTAC 
TTGAAACAAT CCAAGGTTCC ATTAAGTGAA TTTGACTTTT CCTGGTCTAA AATTTCTGAC 
ATTCAGTCTC AGCTTGAGAA ATTGATTGAA AAGAATTACT TTCTTCATAA GTCAGCCCAG 
GAAGCATATA AGTCATACAT ACGAGCCTAT GATTCCCATT CTCTGAAACA GATCTTTAAT 
GTTAATAACC TAAATTTGCC TCAGGTTGCT CTGTCATTTG GTTTCAAGGT GCCTCCCTTC 
GTTGATCTGA ACGTCAACAG TAATGAAGGC AAGCAGAAAA AGCGAGGAGG TGGTGGTGGA 
TTTGGCTACC AGAAAACCAA GAAAGTTGAG AAATCCAAAA TCTTTAAACA CATTAGCAAG 
AAATCATCTG ACAGCAGGCA GTTCTCTCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genomic interaction between ER and HMGB2 identifies DDX18 as a novel driver of endocrine resistance in breast cancer cells.
Oncogene34(29)3871-80(2015 Jul)
Redmond AM,Byrne C,Bane FT,Brown GD,Tibbitts P,O'Brien K,Hill AD,Carroll JS,Young LS

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Ddx18 is essential for cell-cycle progression in zebrafish hematopoietic cells and is mutated in human AML.
Blood118(4)903-15(2011 Jul)
Payne EM,Bolli N,Rhodes J,Abdel-Wahab OI,Levine R,Hedvat CV,Stone R,Khanna-Gupta A,Sun H,Kanki JP,Gazda HT,Beggs AH,Cotter FE,Look AT

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Cell142(5)810-21(2010 Sep)
Ohta S,Bukowski-Wills JC,Sanchez-Pulido L,Alves Fde L,Wood L,Chen ZA,Platani M,Fischer L,Hudson DF,Ponting CP,Fukagawa T,Earnshaw WC,Rappsilber J

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DDX18 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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