NAE1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAE1
Entrez Gene ID	8883
Full Name	NEDD8 activating enzyme E1 subunit 1
Synonyms	A-116A10.1,APPBP1,HPP1,ula-1
General protein information	Preferred Names NEDD8 activating enzyme E1 subunit 1 Names NEDD8-activating enzyme E1 regulatory subunit APP-BP1 NEDD8-activating enzyme E1 subunit amyloid beta precursor protein binding protein 1, 59kDa amyloid beta precursor protein-binding protein 1, 59 kDa amyloid beta precursor protein-binding protein 1, 59kD amyloid protein-binding protein 1 proto-oncogene protein 1 protooncogene protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1
Summary	The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	603385

mRNA and Protein(s)

mRNA	Protein	Name
XM_011523422.1	XP_011521724.1	NEDD8-activating enzyme E1 regulatory subunit isoform X1
NM_001018160.1	NP_001018170.1	NEDD8-activating enzyme E1 regulatory subunit isoform c
NM_003905.3	NP_003896.1	NEDD8-activating enzyme E1 regulatory subunit isoform a
XM_011523422.2	XP_011521724.1	NEDD8-activating enzyme E1 regulatory subunit isoform X1
XM_005256215.1	XP_005256272.1	NEDD8-activating enzyme E1 regulatory subunit isoform X1
NM_003905.4	NP_003896.1	NEDD8-activating enzyme E1 regulatory subunit isoform a
NM_001286500.1	NP_001273429.1	NEDD8-activating enzyme E1 regulatory subunit isoform d
NM_001018159.1	NP_001018169.1	NEDD8-activating enzyme E1 regulatory subunit isoform b

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NAE1	NP_001233317.1
Gallus gallus (chicken)	NAE1	NP_001006129.1
Arabidopsis thaliana (thale cress)	AXL	NP_180800.1
Arabidopsis thaliana (thale cress)	AXR1	NP_172010.1
Homo sapiens (human)	NAE1	NP_001273429.1
	NAE1	XP_001085059.1
Bos taurus (cattle)	NAE1	NP_001179962.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005544	XP_315544.2
Canis lupus familiaris (dog)	NAE1	XP_003639123.1
Rattus norvegicus (Norway rat)	Nae1	NP_114461.2
Danio rerio (zebrafish)	nae1	NP_956793.1
Drosophila melanogaster (fruit fly)	APP-BP1	NP_648435.1
Oryza sativa (rice)	Os03g0820100	NP_001051717.1
Xenopus tropicalis (tropical clawed frog)	nae1	NP_001120050.1
Mus musculus (house mouse)	Nae1	NP_659180.1

Gene Ontology
Bibliography

Related articles in PubMed

Neddylation blockade diminishes hepatic metastasis by dampening cancer stem-like cells and angiogenesis in uveal melanoma.
Jin Y, Zhang P, Wang Y, Jin B, Zhou J, Zhang J, Pan J
Clinical cancer research : an official journal of the American Association for Cancer Research(2017 Dec)

Inhibition of Neddylation Modification Sensitizes Pancreatic Cancer Cells to Gemcitabine.
Li H, Zhou W, Li L, Wu J, Liu X, Zhao L, Jia L, Sun Y
Neoplasia (New York, N.Y.)19(6)509-518(2017 Jun)

Neddylation pathway is up-regulated in human intrahepatic cholangiocarcinoma and serves as a potential therapeutic target.
Gao Q, Yu GY, Shi JY, Li LH, Zhang WJ, Wang ZC, Yang LX, Duan M, Zhao H, Wang XY, Zhou J, Qiu SJ, Jeong LS, Jia LJ, Fan J
Oncotarget5(17)7820-32(2014 Sep)

A metal-based inhibitor of NEDD8-activating enzyme.
Zhong HJ, Yang H, Chan DS, Leung CH, Wang HM, Ma DL
PloS one7(11)e49574(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

E1 (NAE1 and UBA3) and E2 (UBC12) enzymes, as well as global NEDD8 conjugation, were upregulated in over 2/3 of human intrahepatic cholangiocarcinoma
Title: Neddylation pathway is up-regulated in human intrahepatic cholangiocarcinoma and serves as a potential therapeutic target.

our results suggest that APP-BP1 modulates neuronal differentiation by altering gene expression profiles in fetal neural stem cells
Title: Amyloid precursor protein binding protein-1 knockdown reduces neuronal differentiation in fetal neural stem cells.

analysis of a metal-based inhibitor of NEDD8-activating enzyme
Title: A metal-based inhibitor of NEDD8-activating enzyme.

APP-BP1 plays an important role in the cell cycle progression of fetal neural stem cells
Title: Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells.

APP-BP1 displayed decreased expression levels in HPV-positive head and neck squamous cell carcinoma
Title: Biological and clinical relevance of transcriptionally active human papillomavirus (HPV) infection in oropharynx squamous cell carcinoma.

The following NAE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03612	NM_003905.4 Latest version!	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu04251	NM_001018160.1 Latest version!	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu04251	XM_005256215.1 Latest version!	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu04251	XM_011523422.2 Latest version!	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu04250	NM_001018159.1 Latest version!	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu05590	NM_001286500.1 Latest version!	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu04251	XM_011523422.1	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu03612	NM_003905.3	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAE1 ( NM_003905.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003896.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03612
Clone ID Related Accession (Same CDS sequence)	NM_003905.3 , NM_003905.4
Accession Version	NM_003905.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM785899.1 and BC000480.2. On May 20, 2005 this sequence version replaced NM_003905.2. Transcript Variant: This variant (1) encodes the predominant isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U50939.1, SRR1803617.143137.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA TTGGTTCGTT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAACAATT TCTTCCTTCA AAGAAGCAGT 
ATCGGCAAGA ACCGAGCTGA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAGCGATGTC 
TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC CTCATTTTTC 
TGTAGGTTTA CTGTTGTAGT TGCAACTCAG CTTCCTGAAA GCACTTCACT ACGCTTAGCA 
GATGTCCTCT GGAATTCCCA GATTCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCCTATGAT 
TTGGATCATA TGGAAAAAAA GGACCACAGT CATACTCCAT GGATTGTGAT CATAGCTAAA 
TATTTAGCAC AGTGGTATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
GAGGACTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAAA ATGAAAATGG GGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTGCAAA ACGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCCGCTGT GGGTAATCAT 
GTTGCCAAAT TGCTGCAGTC CATTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGTT TGGATACAAT TAACAAGGAT GAAATTATTT CTAGCATGGA CAATCCAGAT 
AATGAAATAG TGTTGTACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAACAGGGT 
AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ATATGGTTTA TCTGTAATGG TGAAAGATGA TTATGTCCAC 
GAATTTTGCC GATATGGAGC TGCTGAGCCA CATACCATTG CTGCATTCTT GGGGGGAGCT 
GCTGCTCAAG AGGTCATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTACAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003896.1
CDS	78..1682
Translation	MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	NM_003905.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003905.3

ORF Insert Sequence:

ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA TTGGTTCGTT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAACAATT TCTTCCTTCA AAGAAGCAGT 
ATCGGCAAGA ACCGAGCTGA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAGCGATGTC 
TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC CTCATTTTTC 
TGTAGGTTTA CTGTTGTAGT TGCAACTCAG CTTCCTGAAA GCACTTCACT ACGCTTAGCA 
GATGTCCTCT GGAATTCCCA GATTCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCCTATGAT 
TTGGATCATA TGGAAAAAAA GGACCACAGT CATACTCCAT GGATTGTGAT CATAGCTAAA 
TATTTAGCAC AGTGGTATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
GAGGACTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAAA ATGAAAATGG GGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTGCAAA ACGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCCGCTGT GGGTAATCAT 
GTTGCCAAAT TGCTGCAGTC CATTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGTT TGGATACAAT TAACAAGGAT GAAATTATTT CTAGCATGGA CAATCCAGAT 
AATGAAATAG TGTTGTACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAACAGGGT 
AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ATATGGTTTA TCTGTAATGG TGAAAGATGA TTATGTCCAC 
GAATTTTGCC GATATGGAGC TGCTGAGCCA CATACCATTG CTGCATTCTT GGGGGGAGCT 
GCTGCTCAAG AGGTCATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTACAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( NM_003905.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003896.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03612
Clone ID Related Accession (Same CDS sequence)	NM_003905.3 , NM_003905.4
Accession Version	NM_003905.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM785899.1 and BC000480.2. On Nov 22, 2018 this sequence version replaced NM_003905.3. Transcript Variant: This variant (1) encodes the predominant isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.483875.1, SRR3476690.780082.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290810.8/ ENSP00000290810.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA TTGGTTCGTT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAACAATT TCTTCCTTCA AAGAAGCAGT 
ATCGGCAAGA ACCGAGCTGA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAGCGATGTC 
TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC CTCATTTTTC 
TGTAGGTTTA CTGTTGTAGT TGCAACTCAG CTTCCTGAAA GCACTTCACT ACGCTTAGCA 
GATGTCCTCT GGAATTCCCA GATTCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCCTATGAT 
TTGGATCATA TGGAAAAAAA GGACCACAGT CATACTCCAT GGATTGTGAT CATAGCTAAA 
TATTTAGCAC AGTGGTATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
GAGGACTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAAA ATGAAAATGG GGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTGCAAA ACGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCCGCTGT GGGTAATCAT 
GTTGCCAAAT TGCTGCAGTC CATTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGTT TGGATACAAT TAACAAGGAT GAAATTATTT CTAGCATGGA CAATCCAGAT 
AATGAAATAG TGTTGTACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAACAGGGT 
AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ATATGGTTTA TCTGTAATGG TGAAAGATGA TTATGTCCAC 
GAATTTTGCC GATATGGAGC TGCTGAGCCA CATACCATTG CTGCATTCTT GGGGGGAGCT 
GCTGCTCAAG AGGTCATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTACAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003896.1
CDS	78..1682
Translation	MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	NM_003905.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003905.4

ORF Insert Sequence:

ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA TTGGTTCGTT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAACAATT TCTTCCTTCA AAGAAGCAGT 
ATCGGCAAGA ACCGAGCTGA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAGCGATGTC 
TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC CTCATTTTTC 
TGTAGGTTTA CTGTTGTAGT TGCAACTCAG CTTCCTGAAA GCACTTCACT ACGCTTAGCA 
GATGTCCTCT GGAATTCCCA GATTCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCCTATGAT 
TTGGATCATA TGGAAAAAAA GGACCACAGT CATACTCCAT GGATTGTGAT CATAGCTAAA 
TATTTAGCAC AGTGGTATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
GAGGACTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAAA ATGAAAATGG GGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTGCAAA ACGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCCGCTGT GGGTAATCAT 
GTTGCCAAAT TGCTGCAGTC CATTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGTT TGGATACAAT TAACAAGGAT GAAATTATTT CTAGCATGGA CAATCCAGAT 
AATGAAATAG TGTTGTACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAACAGGGT 
AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ATATGGTTTA TCTGTAATGG TGAAAGATGA TTATGTCCAC 
GAATTTTGCC GATATGGAGC TGCTGAGCCA CATACCATTG CTGCATTCTT GGGGGGAGCT 
GCTGCTCAAG AGGTCATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTACAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( NM_001018160.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018170.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu04251
Clone ID Related Accession (Same CDS sequence)	XM_011523422.1 , NM_001018160.1 , XM_011523422.2 , XM_005256215.1
Accession Version	NM_001018160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM785899.1 and BC000480.2. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.970425.1, SRR3476690.592289.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
GCAACTTTCC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018170.1
CDS	241..1578
Translation	MEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	NM_001018160.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018160.1

ORF Insert Sequence:

ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
GCAACTTTCC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_011523422.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521724.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu04251
Clone ID Related Accession (Same CDS sequence)	XM_011523422.1 , NM_001018160.1 , XM_011523422.2 , XM_005256215.1
Accession Version	XM_011523422.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X1
Comment

ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
GCAACTTTCC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011521724.1

Translation

MEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_011523422.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523422.1

ORF Insert Sequence:

ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
GCAACTTTCC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_011523422.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521724.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu04251
Clone ID Related Accession (Same CDS sequence)	XM_011523422.1 , NM_001018160.1 , XM_011523422.2 , XM_005256215.1
Accession Version	XM_011523422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
GCAACTTTCC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521724.1
CDS	157..1494
Translation	MEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_011523422.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523422.2

ORF Insert Sequence:

ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
GCAACTTTCC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_005256215.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005256272.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu04251
Clone ID Related Accession (Same CDS sequence)	XM_011523422.1 , NM_001018160.1 , XM_011523422.2 , XM_005256215.1
Accession Version	XM_005256215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
GCAACTTTCC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005256272.1
CDS	315..1652
Translation	MEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_005256215.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005256215.1

ORF Insert Sequence:

ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
GCAACTTTCC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( NM_001018159.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018169.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04250
Clone ID Related Accession (Same CDS sequence)	NM_001018159.1
Accession Version	NM_001018159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM785899.1, BM045535.1 and BC000480.2. Transcript Variant: This variant (2) contains an alternate exon compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.136333.1, SRR1660805.38854.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCTC AGCAAACAAA AACAAATGAA GCCAGGTTGT GGGGTGATCA TGGGCAAGAG 
GCTTTAGAAT CTGCTCATGT TTGCCTAATA AATGCAACAG CCACAGGAAC TGAAATTCTT 
AAAAACTTGG TACTACCAGG TATTGGTTCG TTTACAATTA TTGATGGAAA TCAGGTCAGC 
GGAGAAGATG CTGGAAACAA TTTCTTCCTT CAAAGAAGCA GTATCGGCAA GAACCGAGCT 
GAAGCTGCCA TGGAATTCTT ACAAGAATTA AATAGCGATG TCTCTGGAAG TTTTGTGGAA 
GAGAGTCCAG AAAACCTTCT AGACAATGAT CCCTCATTTT TCTGTAGGTT TACTGTTGTA 
GTTGCAACTC AGCTTCCTGA AAGCACTTCA CTACGCTTAG CAGATGTCCT CTGGAATTCC 
CAGATTCCTC TTTTGATCTG TAGGACATAT GGACTAGTTG GTTATATGAG GATCATTATA 
AAAGAACATC CAGTAATAGA ATCTCATCCA GATAATGCAT TAGAGGATCT ACGACTAGAT 
AAGCCATTTC CTGAACTGAG AGAACATTTT CAGTCCTATG ATTTGGATCA TATGGAAAAA 
AAGGACCACA GTCATACTCC ATGGATTGTG ATCATAGCTA AATATTTAGC ACAGTGGTAT 
AGTGAAACAA ATGGACGAAT ACCTAAAACG TATAAAGAAA AAGAGGACTT CAGAGATTTG 
ATTAGACAAG GAATTCTAAA AAATGAAAAT GGGGCTCCAG AAGATGAAGA GAATTTTGAA 
GAAGCTATTA AAAATGTGAA CACAGCACTA AATACAACTC AGATCCCAAG CAGTATTGAA 
GATATATTTA ATGATGATCG CTGCATAAAT ATCACCAAAC AGACTCCATC ATTTTGGATT 
TTAGCTCGTG CCTTAAAGGA ATTTGTGGCC AAAGAGGGTC AAGGAAATTT ACCTGTTCGA 
GGCACAATTC CTGATATGAT TGCAGATTCA GGCAAATATA TAAAACTGCA AAACGTTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCCGCT GTGGGTAATC ATGTTGCCAA ATTGCTGCAG 
TCCATTGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCTGCATTTC TTCGAGTGGT AAGATGTCGA TCCTTAGCTG AAGAATATGG TTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCTAGCATG GACAATCCAG ATAATGAAAT AGTGTTGTAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAACAGG GTAGATATCC AGGAGTATCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTGG CTTCCTTCAG 
GAATATGGTT TATCTGTAAT GGTGAAAGAT GATTATGTCC ACGAATTTTG CCGATATGGA 
GCTGCTGAGC CACATACCAT TGCTGCATTC TTGGGGGGAG CTGCTGCTCA AGAGGTCATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTACAG TGGCATGTCA 
CAAACTTCAG CAACTTTCCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018169.1
CDS	194..1780
Translation	MDAQQTKTNEARLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	NM_001018159.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018159.1

ORF Insert Sequence:

ATGGATGCTC AGCAAACAAA AACAAATGAA GCCAGGTTGT GGGGTGATCA TGGGCAAGAG 
GCTTTAGAAT CTGCTCATGT TTGCCTAATA AATGCAACAG CCACAGGAAC TGAAATTCTT 
AAAAACTTGG TACTACCAGG TATTGGTTCG TTTACAATTA TTGATGGAAA TCAGGTCAGC 
GGAGAAGATG CTGGAAACAA TTTCTTCCTT CAAAGAAGCA GTATCGGCAA GAACCGAGCT 
GAAGCTGCCA TGGAATTCTT ACAAGAATTA AATAGCGATG TCTCTGGAAG TTTTGTGGAA 
GAGAGTCCAG AAAACCTTCT AGACAATGAT CCCTCATTTT TCTGTAGGTT TACTGTTGTA 
GTTGCAACTC AGCTTCCTGA AAGCACTTCA CTACGCTTAG CAGATGTCCT CTGGAATTCC 
CAGATTCCTC TTTTGATCTG TAGGACATAT GGACTAGTTG GTTATATGAG GATCATTATA 
AAAGAACATC CAGTAATAGA ATCTCATCCA GATAATGCAT TAGAGGATCT ACGACTAGAT 
AAGCCATTTC CTGAACTGAG AGAACATTTT CAGTCCTATG ATTTGGATCA TATGGAAAAA 
AAGGACCACA GTCATACTCC ATGGATTGTG ATCATAGCTA AATATTTAGC ACAGTGGTAT 
AGTGAAACAA ATGGACGAAT ACCTAAAACG TATAAAGAAA AAGAGGACTT CAGAGATTTG 
ATTAGACAAG GAATTCTAAA AAATGAAAAT GGGGCTCCAG AAGATGAAGA GAATTTTGAA 
GAAGCTATTA AAAATGTGAA CACAGCACTA AATACAACTC AGATCCCAAG CAGTATTGAA 
GATATATTTA ATGATGATCG CTGCATAAAT ATCACCAAAC AGACTCCATC ATTTTGGATT 
TTAGCTCGTG CCTTAAAGGA ATTTGTGGCC AAAGAGGGTC AAGGAAATTT ACCTGTTCGA 
GGCACAATTC CTGATATGAT TGCAGATTCA GGCAAATATA TAAAACTGCA AAACGTTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCCGCT GTGGGTAATC ATGTTGCCAA ATTGCTGCAG 
TCCATTGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCTGCATTTC TTCGAGTGGT AAGATGTCGA TCCTTAGCTG AAGAATATGG TTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCTAGCATG GACAATCCAG ATAATGAAAT AGTGTTGTAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAACAGG GTAGATATCC AGGAGTATCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTGG CTTCCTTCAG 
GAATATGGTT TATCTGTAAT GGTGAAAGAT GATTATGTCC ACGAATTTTG CCGATATGGA 
GCTGCTGAGC CACATACCAT TGCTGCATTC TTGGGGGGAG CTGCTGCTCA AGAGGTCATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTACAG TGGCATGTCA 
CAAACTTCAG CAACTTTCCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( NM_001286500.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273429.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu05590
Clone ID Related Accession (Same CDS sequence)	NM_001286500.1
Accession Version	NM_001286500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK097680.1 and U50939.1. On Nov 7, 2013 this sequence version replaced XM_005256214.1. Transcript Variant: This variant (4) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (d) has the same N- and C-termini but contains an additional 3 aa compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK097680.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA ACGTAGGTAT TGGTTCGTTT 
ACAATTATTG ATGGAAATCA GGTCAGCGGA GAAGATGCTG GAAACAATTT CTTCCTTCAA 
AGAAGCAGTA TCGGCAAGAA CCGAGCTGAA GCTGCCATGG AATTCTTACA AGAATTAAAT 
AGCGATGTCT CTGGAAGTTT TGTGGAAGAG AGTCCAGAAA ACCTTCTAGA CAATGATCCC 
TCATTTTTCT GTAGGTTTAC TGTTGTAGTT GCAACTCAGC TTCCTGAAAG CACTTCACTA 
CGCTTAGCAG ATGTCCTCTG GAATTCCCAG ATTCCTCTTT TGATCTGTAG GACATATGGA 
CTAGTTGGTT ATATGAGGAT CATTATAAAA GAACATCCAG TAATAGAATC TCATCCAGAT 
AATGCATTAG AGGATCTACG ACTAGATAAG CCATTTCCTG AACTGAGAGA ACATTTTCAG 
TCCTATGATT TGGATCATAT GGAAAAAAAG GACCACAGTC ATACTCCATG GATTGTGATC 
ATAGCTAAAT ATTTAGCACA GTGGTATAGT GAAACAAATG GACGAATACC TAAAACGTAT 
AAAGAAAAAG AGGACTTCAG AGATTTGATT AGACAAGGAA TTCTAAAAAA TGAAAATGGG 
GCTCCAGAAG ATGAAGAGAA TTTTGAAGAA GCTATTAAAA ATGTGAACAC AGCACTAAAT 
ACAACTCAGA TCCCAAGCAG TATTGAAGAT ATATTTAATG ATGATCGCTG CATAAATATC 
ACCAAACAGA CTCCATCATT TTGGATTTTA GCTCGTGCCT TAAAGGAATT TGTGGCCAAA 
GAGGGTCAAG GAAATTTACC TGTTCGAGGC ACAATTCCTG ATATGATTGC AGATTCAGGC 
AAATATATAA AACTGCAAAA CGTTTACCGT GAAAAAGCAA AGAAAGATGC TGCCGCTGTG 
GGTAATCATG TTGCCAAATT GCTGCAGTCC ATTGGCCAGG CACCAGAGTC CATTTCAGAG 
AAAGAATTAA AATTACTCTG CAGCAATTCT GCATTTCTTC GAGTGGTAAG ATGTCGATCC 
TTAGCTGAAG AATATGGTTT GGATACAATT AACAAGGATG AAATTATTTC TAGCATGGAC 
AATCCAGATA ATGAAATAGT GTTGTACTTA ATGTTACGGG CTGTTGATAG ATTTCATAAA 
CAACAGGGTA GATATCCAGG AGTATCTAAC TATCAAGTTG AAGAAGATAT AGGAAAGTTG 
AAGTCTTGTC TCACTGGCTT CCTTCAGGAA TATGGTTTAT CTGTAATGGT GAAAGATGAT 
TATGTCCACG AATTTTGCCG ATATGGAGCT GCTGAGCCAC ATACCATTGC TGCATTCTTG 
GGGGGAGCTG CTGCTCAAGA GGTCATCAAA ATAATCACCA AACAATTTGT AATTTTTAAT 
AATACTTACA TTTACAGTGG CATGTCACAA ACTTCAGCAA CTTTCCAGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273429.1
CDS	78..1691
Translation	MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGNVGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	NM_001286500.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286500.1

ORF Insert Sequence:

ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA ACGTAGGTAT TGGTTCGTTT 
ACAATTATTG ATGGAAATCA GGTCAGCGGA GAAGATGCTG GAAACAATTT CTTCCTTCAA 
AGAAGCAGTA TCGGCAAGAA CCGAGCTGAA GCTGCCATGG AATTCTTACA AGAATTAAAT 
AGCGATGTCT CTGGAAGTTT TGTGGAAGAG AGTCCAGAAA ACCTTCTAGA CAATGATCCC 
TCATTTTTCT GTAGGTTTAC TGTTGTAGTT GCAACTCAGC TTCCTGAAAG CACTTCACTA 
CGCTTAGCAG ATGTCCTCTG GAATTCCCAG ATTCCTCTTT TGATCTGTAG GACATATGGA 
CTAGTTGGTT ATATGAGGAT CATTATAAAA GAACATCCAG TAATAGAATC TCATCCAGAT 
AATGCATTAG AGGATCTACG ACTAGATAAG CCATTTCCTG AACTGAGAGA ACATTTTCAG 
TCCTATGATT TGGATCATAT GGAAAAAAAG GACCACAGTC ATACTCCATG GATTGTGATC 
ATAGCTAAAT ATTTAGCACA GTGGTATAGT GAAACAAATG GACGAATACC TAAAACGTAT 
AAAGAAAAAG AGGACTTCAG AGATTTGATT AGACAAGGAA TTCTAAAAAA TGAAAATGGG 
GCTCCAGAAG ATGAAGAGAA TTTTGAAGAA GCTATTAAAA ATGTGAACAC AGCACTAAAT 
ACAACTCAGA TCCCAAGCAG TATTGAAGAT ATATTTAATG ATGATCGCTG CATAAATATC 
ACCAAACAGA CTCCATCATT TTGGATTTTA GCTCGTGCCT TAAAGGAATT TGTGGCCAAA 
GAGGGTCAAG GAAATTTACC TGTTCGAGGC ACAATTCCTG ATATGATTGC AGATTCAGGC 
AAATATATAA AACTGCAAAA CGTTTACCGT GAAAAAGCAA AGAAAGATGC TGCCGCTGTG 
GGTAATCATG TTGCCAAATT GCTGCAGTCC ATTGGCCAGG CACCAGAGTC CATTTCAGAG 
AAAGAATTAA AATTACTCTG CAGCAATTCT GCATTTCTTC GAGTGGTAAG ATGTCGATCC 
TTAGCTGAAG AATATGGTTT GGATACAATT AACAAGGATG AAATTATTTC TAGCATGGAC 
AATCCAGATA ATGAAATAGT GTTGTACTTA ATGTTACGGG CTGTTGATAG ATTTCATAAA 
CAACAGGGTA GATATCCAGG AGTATCTAAC TATCAAGTTG AAGAAGATAT AGGAAAGTTG 
AAGTCTTGTC TCACTGGCTT CCTTCAGGAA TATGGTTTAT CTGTAATGGT GAAAGATGAT 
TATGTCCACG AATTTTGCCG ATATGGAGCT GCTGAGCCAC ATACCATTGC TGCATTCTTG 
GGGGGAGCTG CTGCTCAAGA GGTCATCAAA ATAATCACCA AACAATTTGT AATTTTTAAT 
AATACTTACA TTTACAGTGG CATGTCACAA ACTTCAGCAA CTTTCCAGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neddylation blockade diminishes hepatic metastasis by dampening cancer stem-like cells and angiogenesis in uveal melanoma.
Clinical cancer research : an official journal of the American Association for Cancer Research(2017 Dec)
Jin Y,Zhang P,Wang Y,Jin B,Zhou J,Zhang J,Pan J

Inhibition of Neddylation Modification Sensitizes Pancreatic Cancer Cells to Gemcitabine.
Neoplasia (New York, N.Y.)19(6)509-518(2017 Jun)
Li H,Zhou W,Li L,Wu J,Liu X,Zhao L,Jia L,Sun Y

Neddylation pathway is up-regulated in human intrahepatic cholangiocarcinoma and serves as a potential therapeutic target.
Oncotarget5(17)7820-32(2014 Sep)
Gao Q,Yu GY,Shi JY,Li LH,Zhang WJ,Wang ZC,Yang LX,Duan M,Zhao H,Wang XY,Zhou J,Qiu SJ,Jeong LS,Jia LJ,Fan J

A metal-based inhibitor of NEDD8-activating enzyme.
PloS one7(11)e49574(2012)
Zhong HJ,Yang H,Chan DS,Leung CH,Wang HM,Ma DL

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

NAE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報