SPHK1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005257766.2	XP_005257823.1	sphingosine kinase 1 isoform X1
XM_011525439.1	XP_011523741.1	sphingosine kinase 1 isoform X1
NM_001142601.1	NP_001136073.1	sphingosine kinase 1 isoform 3
NM_001142602.2	NP_001136074.1	sphingosine kinase 1 isoform 3
NM_182965.2	NP_892010.2	sphingosine kinase 1 isoform 2
NM_021972.4	NP_068807.2	sphingosine kinase 1 isoform 1
NM_001142601.2	NP_001136073.1	sphingosine kinase 1 isoform 3
NM_182965.3	NP_892010.2	sphingosine kinase 1 isoform 2
NM_021972.3	NP_068807.2	sphingosine kinase 1 isoform 1
NM_001355139.2	NP_001342068.1	sphingosine kinase 1 isoform 3
NM_001142602.1	NP_001136074.1	sphingosine kinase 1 isoform 3
NM_001355139.1	NP_001342068.1	sphingosine kinase 1 isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19935	NM_001142601.2 Latest version!	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19935	NM_001142602.2 Latest version!	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19935	NM_001355139.2 Latest version!	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19985	NM_021972.4 Latest version!	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20341	NM_182965.3 Latest version!	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu19935	XM_005257766.2 Latest version!	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19935	XM_011525439.1 Latest version!	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu19935	NM_001142601.1	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20341	NM_182965.2	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu19985	NM_021972.3	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19935	NM_001142602.1	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu19935	NM_001355139.1	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

SPHK1 ( NM_001142601.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136073.1 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu19935
Clone ID Related Accession (Same CDS sequence)	XM_005257766.2 , XM_011525439.1 , NM_001142601.1 , NM_001142602.2 , NM_001142601.2 , NM_001355139.2 , NM_001142602.1 , NM_001355139.1
Accession Version	NM_001142601.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK022402.1, BC030553.1, DC384776.1 and BC009419.1. Transcript Variant: This variant (3) contains an alternate in-frame segment in the 5' coding region and uses a downstream start codon, compared to variant 2. Isoform 3 has a shorter N-terminus, compared to isoform 2. Variants 3, 4 and 5 encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK022402.1, AK225250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136073.1
CDS	393..1547
Translation	MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_001142601.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142601.1

ORF Insert Sequence:

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( NM_001142602.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136074.1 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu19935
Clone ID Related Accession (Same CDS sequence)	XM_005257766.2 , XM_011525439.1 , NM_001142601.1 , NM_001142602.2 , NM_001142601.2 , NM_001355139.2 , NM_001142602.1 , NM_001355139.1
Accession Version	NM_001142602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP304783.1, BC030553.1 and DC384776.1. On Jun 2, 2019 this sequence version replaced NM_001142602.1. Transcript Variant: This variant (4) contains an alternate in-frame segment in the 5' coding region and uses a downstream start codon, compared to variant 2. Isoform 3 has a shorter N-terminus, compared to isoform 2. Variants 3, 4 and 5 encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF266756.1, AK023393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136074.1
CDS	368..1522
Translation	MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_001142602.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142602.2

ORF Insert Sequence:

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( NM_001142601.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136073.1 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu19935
Clone ID Related Accession (Same CDS sequence)	XM_005257766.2 , XM_011525439.1 , NM_001142601.1 , NM_001142602.2 , NM_001142601.2 , NM_001355139.2 , NM_001142602.1 , NM_001355139.1
Accession Version	NM_001142601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK022402.1, BC030553.1 and DC384776.1. On Jun 2, 2019 this sequence version replaced NM_001142601.1. Transcript Variant: This variant (3) contains an alternate in-frame segment in the 5' coding region and uses a downstream start codon, compared to variant 2. Isoform 3 has a shorter N-terminus, compared to isoform 2. Variants 3, 4 and 5 encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK022402.1, AK225250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136073.1
CDS	393..1547
Translation	MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_001142601.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142601.2

ORF Insert Sequence:

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( NM_001355139.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001342068.1 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu19935
Clone ID Related Accession (Same CDS sequence)	XM_005257766.2 , XM_011525439.1 , NM_001142601.1 , NM_001142602.2 , NM_001142601.2 , NM_001355139.2 , NM_001142602.1 , NM_001355139.1
Accession Version	NM_001355139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA482243.1, DC384776.1 and BC030553.1. On Jun 2, 2019 this sequence version replaced NM_001355139.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF200328.1, AJ245504.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342068.1
CDS	521..1675
Translation	MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_001355139.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355139.2

ORF Insert Sequence:

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( NM_001142602.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136074.1 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 4, mRNA.

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CloneID	OHu19935
Clone ID Related Accession (Same CDS sequence)	XM_005257766.2 , XM_011525439.1 , NM_001142601.1 , NM_001142602.2 , NM_001142601.2 , NM_001355139.2 , NM_001142602.1 , NM_001355139.1
Accession Version	NM_001142602.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP304783.1, BC030553.1, DC384776.1 and BC009419.1. Transcript Variant: This variant (4) contains an alternate in-frame segment in the 5' coding region and uses a downstream start codon, compared to variant 2. Isoform 3 has a shorter N-terminus, compared to isoform 2. Variants 3, 4 and 5 encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ245504.1, AK292614.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136074.1
CDS	438..1592
Translation	MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_001142602.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142602.1

ORF Insert Sequence:

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( NM_001355139.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001342068.1 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 5, mRNA.

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CloneID	OHu19935
Clone ID Related Accession (Same CDS sequence)	XM_005257766.2 , XM_011525439.1 , NM_001142601.1 , NM_001142602.2 , NM_001142601.2 , NM_001355139.2 , NM_001142602.1 , NM_001355139.1
Accession Version	NM_001355139.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK095578.1, AC090699.10, DB174389.1, DA482243.1, DC384776.1, BC030553.1 and BC009419.1. On Sep 16, 2017 this sequence version replaced XM_005257766.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF238083.1, AK292614.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342068.1
CDS	748..1902
Translation	MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_001355139.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355139.1

ORF Insert Sequence:

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( NM_021972.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_068807.2 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 1, mRNA.

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CloneID	OHu19985
Clone ID Related Accession (Same CDS sequence)	NM_021972.4 , NM_021972.3
Accession Version	NM_021972.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK022402.1, BC030553.1, DC384776.1 and BC009419.1. On Jun 2, 2019 this sequence version replaced NM_021972.3. Transcript Variant: This variant (1) has multiple differences in the coding region, compared to variant 2. The resulting protein (isoform 1) has a shorter N-terminus and contains an additional in-frame portion of the 5' coding region, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009419.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCAG TGGTCGGTTG CGGACGTGGC CTCTTTGGTT TTGTTTTCTC AGCGGGCGGC 
CCCCGGGGCG TGCTCCCGCG GCCCTGCCGC GTGCTGGTGC TGCTGAACCC GCGCGGCGGC 
AAGGGCAAGG CCTTGCAGCT CTTCCGGAGT CACGTGCAGC CCCTTTTGGC TGAGGCTGAA 
ATCTCCTTCA CGCTGATGCT CACTGAGCGG CGGAACCACG CGCGGGAGCT GGTGCGGTCG 
GAGGAGCTGG GCCGCTGGGA CGCTCTGGTG GTCATGTCTG GAGACGGGCT GATGCACGAG 
GTGGTGAACG GGCTCATGGA GCGGCCTGAC TGGGAGACCG CCATCCAGAA GCCCCTGTGT 
AGCCTCCCAG CAGGCTCTGG CAACGCGCTG GCAGCTTCCT TGAACCATTA TGCTGGCTAT 
GAGCAGGTCA CCAATGAAGA CCTCCTGACC AACTGCACGC TATTGCTGTG CCGCCGGCTG 
CTGTCACCCA TGAACCTGCT GTCTCTGCAC ACGGCTTCGG GGCTGCGCCT CTTCTCTGTG 
CTCAGCCTGG CCTGGGGCTT CATTGCTGAT GTGGACCTAG AGAGTGAGAA GTATCGGCGT 
CTGGGGGAGA TGCGCTTCAC TCTGGGCACC TTCCTGCGTC TGGCAGCCCT GCGCACCTAC 
CGCGGCCGAC TGGCCTACCT CCCTGTAGGA AGAGTGGGTT CCAAGACACC TGCCTCCCCC 
GTTGTGGTCC AGCAGGGCCC GGTAGATGCA CACCTTGTGC CACTGGAGGA GCCAGTGCCC 
TCTCACTGGA CAGTGGTGCC CGACGAGGAC TTTGTGCTAG TCCTGGCACT GCTGCACTCG 
CACCTGGGCA GTGAGATGTT TGCTGCACCC ATGGGCCGCT GTGCAGCTGG CGTCATGCAT 
CTGTTCTACG TGCGGGCGGG AGTGTCTCGT GCCATGCTGC TGCGCCTCTT CCTGGCCATG 
GAGAAGGGCA GGCATATGGA GTATGAATGC CCCTACTTGG TATATGTGCC CGTGGTCGCC 
TTCCGCTTGG AGCCCAAGGA TGGGAAAGGT GTGTTTGCAG TGGATGGGGA ATTGATGGTT 
AGCGAGGCCG TGCAGGGCCA GGTGCACCCA AACTACTTCT GGATGGTCAG CGGTTGCGTG 
GAGCCCCCGC CCAGCTGGAA GCCCCAGCAG ATGCCACCGC CAGAAGAGCC CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068807.2
CDS	393..1589
Translation	MDPVVGCGRGLFGFVFSAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_021972.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021972.4

ORF Insert Sequence:

ATGGATCCAG TGGTCGGTTG CGGACGTGGC CTCTTTGGTT TTGTTTTCTC AGCGGGCGGC 
CCCCGGGGCG TGCTCCCGCG GCCCTGCCGC GTGCTGGTGC TGCTGAACCC GCGCGGCGGC 
AAGGGCAAGG CCTTGCAGCT CTTCCGGAGT CACGTGCAGC CCCTTTTGGC TGAGGCTGAA 
ATCTCCTTCA CGCTGATGCT CACTGAGCGG CGGAACCACG CGCGGGAGCT GGTGCGGTCG 
GAGGAGCTGG GCCGCTGGGA CGCTCTGGTG GTCATGTCTG GAGACGGGCT GATGCACGAG 
GTGGTGAACG GGCTCATGGA GCGGCCTGAC TGGGAGACCG CCATCCAGAA GCCCCTGTGT 
AGCCTCCCAG CAGGCTCTGG CAACGCGCTG GCAGCTTCCT TGAACCATTA TGCTGGCTAT 
GAGCAGGTCA CCAATGAAGA CCTCCTGACC AACTGCACGC TATTGCTGTG CCGCCGGCTG 
CTGTCACCCA TGAACCTGCT GTCTCTGCAC ACGGCTTCGG GGCTGCGCCT CTTCTCTGTG 
CTCAGCCTGG CCTGGGGCTT CATTGCTGAT GTGGACCTAG AGAGTGAGAA GTATCGGCGT 
CTGGGGGAGA TGCGCTTCAC TCTGGGCACC TTCCTGCGTC TGGCAGCCCT GCGCACCTAC 
CGCGGCCGAC TGGCCTACCT CCCTGTAGGA AGAGTGGGTT CCAAGACACC TGCCTCCCCC 
GTTGTGGTCC AGCAGGGCCC GGTAGATGCA CACCTTGTGC CACTGGAGGA GCCAGTGCCC 
TCTCACTGGA CAGTGGTGCC CGACGAGGAC TTTGTGCTAG TCCTGGCACT GCTGCACTCG 
CACCTGGGCA GTGAGATGTT TGCTGCACCC ATGGGCCGCT GTGCAGCTGG CGTCATGCAT 
CTGTTCTACG TGCGGGCGGG AGTGTCTCGT GCCATGCTGC TGCGCCTCTT CCTGGCCATG 
GAGAAGGGCA GGCATATGGA GTATGAATGC CCCTACTTGG TATATGTGCC CGTGGTCGCC 
TTCCGCTTGG AGCCCAAGGA TGGGAAAGGT GTGTTTGCAG TGGATGGGGA ATTGATGGTT 
AGCGAGGCCG TGCAGGGCCA GGTGCACCCA AACTACTTCT GGATGGTCAG CGGTTGCGTG 
GAGCCCCCGC CCAGCTGGAA GCCCCAGCAG ATGCCACCGC CAGAAGAGCC CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( NM_021972.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_068807.2 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 1, mRNA.

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CloneID	OHu19985
Clone ID Related Accession (Same CDS sequence)	NM_021972.4 , NM_021972.3
Accession Version	NM_021972.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK022402.1, BC030553.1, DC384776.1 and BC009419.1. On Dec 12, 2008 this sequence version replaced NM_021972.2. Transcript Variant: This variant (1) has multiple differences in the coding region, compared to variant 2. The resulting protein (isoform 1) has a shorter N-terminus and contains an additional in-frame portion of the 5' coding region, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009419.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCAG TGGTCGGTTG CGGACGTGGC CTCTTTGGTT TTGTTTTCTC AGCGGGCGGC 
CCCCGGGGCG TGCTCCCGCG GCCCTGCCGC GTGCTGGTGC TGCTGAACCC GCGCGGCGGC 
AAGGGCAAGG CCTTGCAGCT CTTCCGGAGT CACGTGCAGC CCCTTTTGGC TGAGGCTGAA 
ATCTCCTTCA CGCTGATGCT CACTGAGCGG CGGAACCACG CGCGGGAGCT GGTGCGGTCG 
GAGGAGCTGG GCCGCTGGGA CGCTCTGGTG GTCATGTCTG GAGACGGGCT GATGCACGAG 
GTGGTGAACG GGCTCATGGA GCGGCCTGAC TGGGAGACCG CCATCCAGAA GCCCCTGTGT 
AGCCTCCCAG CAGGCTCTGG CAACGCGCTG GCAGCTTCCT TGAACCATTA TGCTGGCTAT 
GAGCAGGTCA CCAATGAAGA CCTCCTGACC AACTGCACGC TATTGCTGTG CCGCCGGCTG 
CTGTCACCCA TGAACCTGCT GTCTCTGCAC ACGGCTTCGG GGCTGCGCCT CTTCTCTGTG 
CTCAGCCTGG CCTGGGGCTT CATTGCTGAT GTGGACCTAG AGAGTGAGAA GTATCGGCGT 
CTGGGGGAGA TGCGCTTCAC TCTGGGCACC TTCCTGCGTC TGGCAGCCCT GCGCACCTAC 
CGCGGCCGAC TGGCCTACCT CCCTGTAGGA AGAGTGGGTT CCAAGACACC TGCCTCCCCC 
GTTGTGGTCC AGCAGGGCCC GGTAGATGCA CACCTTGTGC CACTGGAGGA GCCAGTGCCC 
TCTCACTGGA CAGTGGTGCC CGACGAGGAC TTTGTGCTAG TCCTGGCACT GCTGCACTCG 
CACCTGGGCA GTGAGATGTT TGCTGCACCC ATGGGCCGCT GTGCAGCTGG CGTCATGCAT 
CTGTTCTACG TGCGGGCGGG AGTGTCTCGT GCCATGCTGC TGCGCCTCTT CCTGGCCATG 
GAGAAGGGCA GGCATATGGA GTATGAATGC CCCTACTTGG TATATGTGCC CGTGGTCGCC 
TTCCGCTTGG AGCCCAAGGA TGGGAAAGGT GTGTTTGCAG TGGATGGGGA ATTGATGGTT 
AGCGAGGCCG TGCAGGGCCA GGTGCACCCA AACTACTTCT GGATGGTCAG CGGTTGCGTG 
GAGCCCCCGC CCAGCTGGAA GCCCCAGCAG ATGCCACCGC CAGAAGAGCC CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068807.2
CDS	393..1589
Translation	MDPVVGCGRGLFGFVFSAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_021972.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021972.3

ORF Insert Sequence:

ATGGATCCAG TGGTCGGTTG CGGACGTGGC CTCTTTGGTT TTGTTTTCTC AGCGGGCGGC 
CCCCGGGGCG TGCTCCCGCG GCCCTGCCGC GTGCTGGTGC TGCTGAACCC GCGCGGCGGC 
AAGGGCAAGG CCTTGCAGCT CTTCCGGAGT CACGTGCAGC CCCTTTTGGC TGAGGCTGAA 
ATCTCCTTCA CGCTGATGCT CACTGAGCGG CGGAACCACG CGCGGGAGCT GGTGCGGTCG 
GAGGAGCTGG GCCGCTGGGA CGCTCTGGTG GTCATGTCTG GAGACGGGCT GATGCACGAG 
GTGGTGAACG GGCTCATGGA GCGGCCTGAC TGGGAGACCG CCATCCAGAA GCCCCTGTGT 
AGCCTCCCAG CAGGCTCTGG CAACGCGCTG GCAGCTTCCT TGAACCATTA TGCTGGCTAT 
GAGCAGGTCA CCAATGAAGA CCTCCTGACC AACTGCACGC TATTGCTGTG CCGCCGGCTG 
CTGTCACCCA TGAACCTGCT GTCTCTGCAC ACGGCTTCGG GGCTGCGCCT CTTCTCTGTG 
CTCAGCCTGG CCTGGGGCTT CATTGCTGAT GTGGACCTAG AGAGTGAGAA GTATCGGCGT 
CTGGGGGAGA TGCGCTTCAC TCTGGGCACC TTCCTGCGTC TGGCAGCCCT GCGCACCTAC 
CGCGGCCGAC TGGCCTACCT CCCTGTAGGA AGAGTGGGTT CCAAGACACC TGCCTCCCCC 
GTTGTGGTCC AGCAGGGCCC GGTAGATGCA CACCTTGTGC CACTGGAGGA GCCAGTGCCC 
TCTCACTGGA CAGTGGTGCC CGACGAGGAC TTTGTGCTAG TCCTGGCACT GCTGCACTCG 
CACCTGGGCA GTGAGATGTT TGCTGCACCC ATGGGCCGCT GTGCAGCTGG CGTCATGCAT 
CTGTTCTACG TGCGGGCGGG AGTGTCTCGT GCCATGCTGC TGCGCCTCTT CCTGGCCATG 
GAGAAGGGCA GGCATATGGA GTATGAATGC CCCTACTTGG TATATGTGCC CGTGGTCGCC 
TTCCGCTTGG AGCCCAAGGA TGGGAAAGGT GTGTTTGCAG TGGATGGGGA ATTGATGGTT 
AGCGAGGCCG TGCAGGGCCA GGTGCACCCA AACTACTTCT GGATGGTCAG CGGTTGCGTG 
GAGCCCCCGC CCAGCTGGAA GCCCCAGCAG ATGCCACCGC CAGAAGAGCC CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( NM_182965.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_892010.2 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 2, mRNA.

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CloneID	OHu20341
Clone ID Related Accession (Same CDS sequence)	NM_182965.2 , NM_182965.3
Accession Version	NM_182965.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK022402.1, BC030553.1, DC384776.1 and BC009419.1. On Dec 11, 2008 this sequence version replaced NM_182965.1. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030553.1, AK292294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCTC AAGTTCTGGG ATTTTTACGC AGCTGGACTC CCCTCCCCCT GGCAGCCCCG 
AGGGGTCCAG CCGCCGCAGG GAATGACGCC GGTGCTCCTG CAGCCACGGC TCCGGGCGGG 
GAAGGCGAGC CCCACAGCCG GCCCTGCGAC GCCCGCCTGG GCAGCACCGA TAAGGAGCTG 
AAGGCAGGAG CCGCCGCCAC GGGCAGCGCC CCCACAGCGC CAGGGACCCC CTGGCAGCGG 
GAGCCGCGGG TCGAGGTTAT GGATCCAGCG GGCGGCCCCC GGGGCGTGCT CCCGCGGCCC 
TGCCGCGTGC TGGTGCTGCT GAACCCGCGC GGCGGCAAGG GCAAGGCCTT GCAGCTCTTC 
CGGAGTCACG TGCAGCCCCT TTTGGCTGAG GCTGAAATCT CCTTCACGCT GATGCTCACT 
GAGCGGCGGA ACCACGCGCG GGAGCTGGTG CGGTCGGAGG AGCTGGGCCG CTGGGACGCT 
CTGGTGGTCA TGTCTGGAGA CGGGCTGATG CACGAGGTGG TGAACGGGCT CATGGAGCGG 
CCTGACTGGG AGACCGCCAT CCAGAAGCCC CTGTGTAGCC TCCCAGCAGG CTCTGGCAAC 
GCGCTGGCAG CTTCCTTGAA CCATTATGCT GGCTATGAGC AGGTCACCAA TGAAGACCTC 
CTGACCAACT GCACGCTATT GCTGTGCCGC CGGCTGCTGT CACCCATGAA CCTGCTGTCT 
CTGCACACGG CTTCGGGGCT GCGCCTCTTC TCTGTGCTCA GCCTGGCCTG GGGCTTCATT 
GCTGATGTGG ACCTAGAGAG TGAGAAGTAT CGGCGTCTGG GGGAGATGCG CTTCACTCTG 
GGCACCTTCC TGCGTCTGGC AGCCCTGCGC ACCTACCGCG GCCGACTGGC CTACCTCCCT 
GTAGGAAGAG TGGGTTCCAA GACACCTGCC TCCCCCGTTG TGGTCCAGCA GGGCCCGGTA 
GATGCACACC TTGTGCCACT GGAGGAGCCA GTGCCCTCTC ACTGGACAGT GGTGCCCGAC 
GAGGACTTTG TGCTAGTCCT GGCACTGCTG CACTCGCACC TGGGCAGTGA GATGTTTGCT 
GCACCCATGG GCCGCTGTGC AGCTGGCGTC ATGCATCTGT TCTACGTGCG GGCGGGAGTG 
TCTCGTGCCA TGCTGCTGCG CCTCTTCCTG GCCATGGAGA AGGGCAGGCA TATGGAGTAT 
GAATGCCCCT ACTTGGTATA TGTGCCCGTG GTCGCCTTCC GCTTGGAGCC CAAGGATGGG 
AAAGGTGTGT TTGCAGTGGA TGGGGAATTG ATGGTTAGCG AGGCCGTGCA GGGCCAGGTG 
CACCCAAACT ACTTCTGGAT GGTCAGCGGT TGCGTGGAGC CCCCGCCCAG CTGGAAGCCC 
CAGCAGATGC CACCGCCAGA AGAGCCCTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_892010.2
CDS	498..1910
Translation	MSAQVLGFLRSWTPLPLAAPRGPAAAGNDAGAPAATAPGGEGEPHSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_182965.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182965.2

ORF Insert Sequence:

ATGTCCGCTC AAGTTCTGGG ATTTTTACGC AGCTGGACTC CCCTCCCCCT GGCAGCCCCG 
AGGGGTCCAG CCGCCGCAGG GAATGACGCC GGTGCTCCTG CAGCCACGGC TCCGGGCGGG 
GAAGGCGAGC CCCACAGCCG GCCCTGCGAC GCCCGCCTGG GCAGCACCGA TAAGGAGCTG 
AAGGCAGGAG CCGCCGCCAC GGGCAGCGCC CCCACAGCGC CAGGGACCCC CTGGCAGCGG 
GAGCCGCGGG TCGAGGTTAT GGATCCAGCG GGCGGCCCCC GGGGCGTGCT CCCGCGGCCC 
TGCCGCGTGC TGGTGCTGCT GAACCCGCGC GGCGGCAAGG GCAAGGCCTT GCAGCTCTTC 
CGGAGTCACG TGCAGCCCCT TTTGGCTGAG GCTGAAATCT CCTTCACGCT GATGCTCACT 
GAGCGGCGGA ACCACGCGCG GGAGCTGGTG CGGTCGGAGG AGCTGGGCCG CTGGGACGCT 
CTGGTGGTCA TGTCTGGAGA CGGGCTGATG CACGAGGTGG TGAACGGGCT CATGGAGCGG 
CCTGACTGGG AGACCGCCAT CCAGAAGCCC CTGTGTAGCC TCCCAGCAGG CTCTGGCAAC 
GCGCTGGCAG CTTCCTTGAA CCATTATGCT GGCTATGAGC AGGTCACCAA TGAAGACCTC 
CTGACCAACT GCACGCTATT GCTGTGCCGC CGGCTGCTGT CACCCATGAA CCTGCTGTCT 
CTGCACACGG CTTCGGGGCT GCGCCTCTTC TCTGTGCTCA GCCTGGCCTG GGGCTTCATT 
GCTGATGTGG ACCTAGAGAG TGAGAAGTAT CGGCGTCTGG GGGAGATGCG CTTCACTCTG 
GGCACCTTCC TGCGTCTGGC AGCCCTGCGC ACCTACCGCG GCCGACTGGC CTACCTCCCT 
GTAGGAAGAG TGGGTTCCAA GACACCTGCC TCCCCCGTTG TGGTCCAGCA GGGCCCGGTA 
GATGCACACC TTGTGCCACT GGAGGAGCCA GTGCCCTCTC ACTGGACAGT GGTGCCCGAC 
GAGGACTTTG TGCTAGTCCT GGCACTGCTG CACTCGCACC TGGGCAGTGA GATGTTTGCT 
GCACCCATGG GCCGCTGTGC AGCTGGCGTC ATGCATCTGT TCTACGTGCG GGCGGGAGTG 
TCTCGTGCCA TGCTGCTGCG CCTCTTCCTG GCCATGGAGA AGGGCAGGCA TATGGAGTAT 
GAATGCCCCT ACTTGGTATA TGTGCCCGTG GTCGCCTTCC GCTTGGAGCC CAAGGATGGG 
AAAGGTGTGT TTGCAGTGGA TGGGGAATTG ATGGTTAGCG AGGCCGTGCA GGGCCAGGTG 
CACCCAAACT ACTTCTGGAT GGTCAGCGGT TGCGTGGAGC CCCCGCCCAG CTGGAAGCCC 
CAGCAGATGC CACCGCCAGA AGAGCCCTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( NM_182965.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_892010.2 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 2, mRNA.

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CloneID	OHu20341
Clone ID Related Accession (Same CDS sequence)	NM_182965.2 , NM_182965.3
Accession Version	NM_182965.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	sphingosine kinase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK022402.1, BC030553.1 and DC384776.1. On Jun 2, 2019 this sequence version replaced NM_182965.2. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030553.1, AK292294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCTC AAGTTCTGGG ATTTTTACGC AGCTGGACTC CCCTCCCCCT GGCAGCCCCG 
AGGGGTCCAG CCGCCGCAGG GAATGACGCC GGTGCTCCTG CAGCCACGGC TCCGGGCGGG 
GAAGGCGAGC CCCACAGCCG GCCCTGCGAC GCCCGCCTGG GCAGCACCGA TAAGGAGCTG 
AAGGCAGGAG CCGCCGCCAC GGGCAGCGCC CCCACAGCGC CAGGGACCCC CTGGCAGCGG 
GAGCCGCGGG TCGAGGTTAT GGATCCAGCG GGCGGCCCCC GGGGCGTGCT CCCGCGGCCC 
TGCCGCGTGC TGGTGCTGCT GAACCCGCGC GGCGGCAAGG GCAAGGCCTT GCAGCTCTTC 
CGGAGTCACG TGCAGCCCCT TTTGGCTGAG GCTGAAATCT CCTTCACGCT GATGCTCACT 
GAGCGGCGGA ACCACGCGCG GGAGCTGGTG CGGTCGGAGG AGCTGGGCCG CTGGGACGCT 
CTGGTGGTCA TGTCTGGAGA CGGGCTGATG CACGAGGTGG TGAACGGGCT CATGGAGCGG 
CCTGACTGGG AGACCGCCAT CCAGAAGCCC CTGTGTAGCC TCCCAGCAGG CTCTGGCAAC 
GCGCTGGCAG CTTCCTTGAA CCATTATGCT GGCTATGAGC AGGTCACCAA TGAAGACCTC 
CTGACCAACT GCACGCTATT GCTGTGCCGC CGGCTGCTGT CACCCATGAA CCTGCTGTCT 
CTGCACACGG CTTCGGGGCT GCGCCTCTTC TCTGTGCTCA GCCTGGCCTG GGGCTTCATT 
GCTGATGTGG ACCTAGAGAG TGAGAAGTAT CGGCGTCTGG GGGAGATGCG CTTCACTCTG 
GGCACCTTCC TGCGTCTGGC AGCCCTGCGC ACCTACCGCG GCCGACTGGC CTACCTCCCT 
GTAGGAAGAG TGGGTTCCAA GACACCTGCC TCCCCCGTTG TGGTCCAGCA GGGCCCGGTA 
GATGCACACC TTGTGCCACT GGAGGAGCCA GTGCCCTCTC ACTGGACAGT GGTGCCCGAC 
GAGGACTTTG TGCTAGTCCT GGCACTGCTG CACTCGCACC TGGGCAGTGA GATGTTTGCT 
GCACCCATGG GCCGCTGTGC AGCTGGCGTC ATGCATCTGT TCTACGTGCG GGCGGGAGTG 
TCTCGTGCCA TGCTGCTGCG CCTCTTCCTG GCCATGGAGA AGGGCAGGCA TATGGAGTAT 
GAATGCCCCT ACTTGGTATA TGTGCCCGTG GTCGCCTTCC GCTTGGAGCC CAAGGATGGG 
AAAGGTGTGT TTGCAGTGGA TGGGGAATTG ATGGTTAGCG AGGCCGTGCA GGGCCAGGTG 
CACCCAAACT ACTTCTGGAT GGTCAGCGGT TGCGTGGAGC CCCCGCCCAG CTGGAAGCCC 
CAGCAGATGC CACCGCCAGA AGAGCCCTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_892010.2
CDS	498..1910
Translation	MSAQVLGFLRSWTPLPLAAPRGPAAAGNDAGAPAATAPGGEGEPHSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	NM_182965.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182965.3

ORF Insert Sequence:

ATGTCCGCTC AAGTTCTGGG ATTTTTACGC AGCTGGACTC CCCTCCCCCT GGCAGCCCCG 
AGGGGTCCAG CCGCCGCAGG GAATGACGCC GGTGCTCCTG CAGCCACGGC TCCGGGCGGG 
GAAGGCGAGC CCCACAGCCG GCCCTGCGAC GCCCGCCTGG GCAGCACCGA TAAGGAGCTG 
AAGGCAGGAG CCGCCGCCAC GGGCAGCGCC CCCACAGCGC CAGGGACCCC CTGGCAGCGG 
GAGCCGCGGG TCGAGGTTAT GGATCCAGCG GGCGGCCCCC GGGGCGTGCT CCCGCGGCCC 
TGCCGCGTGC TGGTGCTGCT GAACCCGCGC GGCGGCAAGG GCAAGGCCTT GCAGCTCTTC 
CGGAGTCACG TGCAGCCCCT TTTGGCTGAG GCTGAAATCT CCTTCACGCT GATGCTCACT 
GAGCGGCGGA ACCACGCGCG GGAGCTGGTG CGGTCGGAGG AGCTGGGCCG CTGGGACGCT 
CTGGTGGTCA TGTCTGGAGA CGGGCTGATG CACGAGGTGG TGAACGGGCT CATGGAGCGG 
CCTGACTGGG AGACCGCCAT CCAGAAGCCC CTGTGTAGCC TCCCAGCAGG CTCTGGCAAC 
GCGCTGGCAG CTTCCTTGAA CCATTATGCT GGCTATGAGC AGGTCACCAA TGAAGACCTC 
CTGACCAACT GCACGCTATT GCTGTGCCGC CGGCTGCTGT CACCCATGAA CCTGCTGTCT 
CTGCACACGG CTTCGGGGCT GCGCCTCTTC TCTGTGCTCA GCCTGGCCTG GGGCTTCATT 
GCTGATGTGG ACCTAGAGAG TGAGAAGTAT CGGCGTCTGG GGGAGATGCG CTTCACTCTG 
GGCACCTTCC TGCGTCTGGC AGCCCTGCGC ACCTACCGCG GCCGACTGGC CTACCTCCCT 
GTAGGAAGAG TGGGTTCCAA GACACCTGCC TCCCCCGTTG TGGTCCAGCA GGGCCCGGTA 
GATGCACACC TTGTGCCACT GGAGGAGCCA GTGCCCTCTC ACTGGACAGT GGTGCCCGAC 
GAGGACTTTG TGCTAGTCCT GGCACTGCTG CACTCGCACC TGGGCAGTGA GATGTTTGCT 
GCACCCATGG GCCGCTGTGC AGCTGGCGTC ATGCATCTGT TCTACGTGCG GGCGGGAGTG 
TCTCGTGCCA TGCTGCTGCG CCTCTTCCTG GCCATGGAGA AGGGCAGGCA TATGGAGTAT 
GAATGCCCCT ACTTGGTATA TGTGCCCGTG GTCGCCTTCC GCTTGGAGCC CAAGGATGGG 
AAAGGTGTGT TTGCAGTGGA TGGGGAATTG ATGGTTAGCG AGGCCGTGCA GGGCCAGGTG 
CACCCAAACT ACTTCTGGAT GGTCAGCGGT TGCGTGGAGC CCCCGCCCAG CTGGAAGCCC 
CAGCAGATGC CACCGCCAGA AGAGCCCTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( XM_005257766.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257823.1 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant X1, mRNA.

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CloneID	OHu19935
Clone ID Related Accession (Same CDS sequence)	XM_005257766.2 , XM_011525439.1 , NM_001142601.1 , NM_001142602.2 , NM_001142601.2 , NM_001355139.2 , NM_001142602.1 , NM_001355139.1
Accession Version	XM_005257766.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	sphingosine kinase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005257766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257823.1
CDS	748..1902
Translation	MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	XM_005257766.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257766.2

ORF Insert Sequence:

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHK1 ( XM_011525439.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523741.1 Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant X2, mRNA.

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CloneID	OHu19935
Clone ID Related Accession (Same CDS sequence)	XM_005257766.2 , XM_011525439.1 , NM_001142601.1 , NM_001142602.2 , NM_001142601.2 , NM_001355139.2 , NM_001142602.1 , NM_001355139.1
Accession Version	XM_011525439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sphingosine kinase 1 isoform X1
Comment

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523741.1
Translation	MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Target ORF information:

RefSeq Version	XM_011525439.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sphingosine kinase 1 (SPHK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525439.1

ORF Insert Sequence:

ATGGATCCAG CGGGCGGCCC CCGGGGCGTG CTCCCGCGGC CCTGCCGCGT GCTGGTGCTG 
CTGAACCCGC GCGGCGGCAA GGGCAAGGCC TTGCAGCTCT TCCGGAGTCA CGTGCAGCCC 
CTTTTGGCTG AGGCTGAAAT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCG 
CGGGAGCTGG TGCGGTCGGA GGAGCTGGGC CGCTGGGACG CTCTGGTGGT CATGTCTGGA 
GACGGGCTGA TGCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC 
ATCCAGAAGC CCCTGTGTAG CCTCCCAGCA GGCTCTGGCA ACGCGCTGGC AGCTTCCTTG 
AACCATTATG CTGGCTATGA GCAGGTCACC AATGAAGACC TCCTGACCAA CTGCACGCTA 
TTGCTGTGCC GCCGGCTGCT GTCACCCATG AACCTGCTGT CTCTGCACAC GGCTTCGGGG 
CTGCGCCTCT TCTCTGTGCT CAGCCTGGCC TGGGGCTTCA TTGCTGATGT GGACCTAGAG 
AGTGAGAAGT ATCGGCGTCT GGGGGAGATG CGCTTCACTC TGGGCACCTT CCTGCGTCTG 
GCAGCCCTGC GCACCTACCG CGGCCGACTG GCCTACCTCC CTGTAGGAAG AGTGGGTTCC 
AAGACACCTG CCTCCCCCGT TGTGGTCCAG CAGGGCCCGG TAGATGCACA CCTTGTGCCA 
CTGGAGGAGC CAGTGCCCTC TCACTGGACA GTGGTGCCCG ACGAGGACTT TGTGCTAGTC 
CTGGCACTGC TGCACTCGCA CCTGGGCAGT GAGATGTTTG CTGCACCCAT GGGCCGCTGT 
GCAGCTGGCG TCATGCATCT GTTCTACGTG CGGGCGGGAG TGTCTCGTGC CATGCTGCTG 
CGCCTCTTCC TGGCCATGGA GAAGGGCAGG CATATGGAGT ATGAATGCCC CTACTTGGTA 
TATGTGCCCG TGGTCGCCTT CCGCTTGGAG CCCAAGGATG GGAAAGGTGT GTTTGCAGTG 
GATGGGGAAT TGATGGTTAG CGAGGCCGTG CAGGGCCAGG TGCACCCAAA CTACTTCTGG 
ATGGTCAGCG GTTGCGTGGA GCCCCCGCCC AGCTGGAAGC CCCAGCAGAT GCCACCGCCA 
GAAGAGCCCT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPHK1
Entrez Gene ID	8877
Full Name	sphingosine kinase 1
Synonyms	SPHK
General protein information	Preferred Names sphingosine kinase 1 Names sphingosine kinase 1 SK 1 SPK 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.1
Summary	The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017].
Disorder MIM:	603730

	LOC710441	XP_001099066.2
Bos taurus (cattle)	SPHK1	XP_002696250.3
Xenopus tropicalis (tropical clawed frog)	sphk1	NP_001016918.1
Gallus gallus (chicken)	SPHK1	XP_004946176.1
Pan troglodytes (chimpanzee)	SPHK1	XP_003953242.1
Canis lupus familiaris (dog)	SPHK1	XP_540448.3
Homo sapiens (human)	SPHK1	NP_892010.2
Mus musculus (house mouse)	Sphk1	NP_079643.2
Rattus norvegicus (Norway rat)	Sphk1	NP_001257740.1
Danio rerio (zebrafish)	LOC100331840	XP_002667585.2
Drosophila melanogaster (fruit fly)	Sk2	NP_647762.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006995	XP_308775.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPHK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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