BANF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BANF1
Entrez Gene ID	8815
Full Name	barrier to autointegration factor 1
Synonyms	BAF,BCRP1,D14S1460,NGPS
General protein information	Preferred Names barrier to autointegration factor 1 Names barrier-to-autointegration factor breakpoint cluster region protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.1
Summary	The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Jan 2009].
Disorder MIM:	603811
Disorder Html:	Nestor-Guillermo progeria syndrome, 614008 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001143985.1	NP_001137457.1	barrier-to-autointegration factor
NM_003860.3	NP_003851.1	barrier-to-autointegration factor
XM_017018515.1	XP_016874004.1	barrier-to-autointegration factor isoform X1
XM_017018515.2	XP_016874004.1	barrier-to-autointegration factor isoform X1
NM_003860.4	NP_003851.1	barrier-to-autointegration factor
XM_017018514.1	XP_016874003.1	barrier-to-autointegration factor isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	banf1	NP_991125.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008753	XP_314872.1
Rattus norvegicus (Norway rat)	Banf1	NP_446083.1
Drosophila melanogaster (fruit fly)	baf	NP_609176.1
Pan troglodytes (chimpanzee)	BANF1	XP_003339255.1
Canis lupus familiaris (dog)	BANF1	XP_854776.1
Xenopus tropicalis (tropical clawed frog)	banf1	XP_002940546.1
Homo sapiens (human)	BANF1	NP_003851.1
Mus musculus (house mouse)	Banf1	NP_001033320.1
Caenorhabditis elegans (roundworm)	baf-1	NP_499085.1
	BANF1	XP_001111884.2
Bos taurus (cattle)	BANF1	NP_892033.1

Gene Ontology
Bibliography

Related articles in PubMed

LEM-domain proteins are lost during human spermiogenesis but BAF and BAF-L persist.
Elkhatib RA, Paci M, Boissier R, Longepied G, Auguste Y, Achard V, Bourgeois P, Levy N, Branger N, Mitchell MJ, Metzler-Guillemain C
Reproduction (Cambridge, England)154(4)387-401(2017 Oct)

VRK2A is an A-type lamin-dependent nuclear envelope kinase that phosphorylates BAF.
Birendra Kc, May DG, Benson BV, Kim DI, Shivega WG, Ali MH, Faustino RS, Campos AR, Roux KJ
Molecular biology of the cell28(17)2241-2250(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings support the value of using BioID to identify unrecognized constituents of distinct subcellular compartments refractory to biochemical isolation and reveal VRK2A as a transmembrane kinase in the NE that regulates BAF.
Title: VRK2A is an A-type lamin-dependent nuclear envelope kinase that phosphorylates BAF.

It has been concluded that the LEM domain, responsible for binding to the chromatin protein BAF, undergoes a conformational change during self-assembly of emerin N-terminal region.
Title: Emerin self-assembly mechanism: role of the LEM domain.

antiviral capabilities of the barrier to autointegration factor (BAF/BANF1) and how its function and regulation places BAF at the junction of multiple pathways protecting a cell's genetic integrity
Title: The Barrier to Autointegration Factor: Interlocking Antiviral Defense with Genome Maintenance.

we demonstrate that BAF is necessary to modulate prelamin A effects on chromatin structure
Title: Barrier-to-autointegration factor (BAF) involvement in prelamin A-related chromatin organization changes.

Vaccinia virus B1 kinase is needed for multiple critical junctures in the poxviral life cycle in a manner that is both dependent on and independent of BAF.
Title: Vaccinia Virus B1 Kinase Is Required for Postreplicative Stages of the Viral Life Cycle in a BAF-Independent Manner in U2OS Cells.

The following BANF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BANF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15955	NM_001143985.1 Latest version!	Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15955	NM_003860.4 Latest version!	Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15955	XM_017018515.2 Latest version!	Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15955	XM_017018514.1 Latest version!	Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu15955	XM_017018515.1	Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15955	NM_003860.3	Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BANF1 ( NM_001143985.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137457.1 Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15955
Clone ID Related Accession (Same CDS sequence)	NM_001143985.1 , NM_003860.3 , XM_017018515.1 , XM_017018515.2 , NM_003860.4 , XM_017018514.1
Accession Version	NM_001143985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	barrier-to-autointegration factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF068235.1, BG748852.1, BU940708.1 and BC008705.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.378181.1, SRR1163658.2965.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137457.1
CDS	452..721
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	NM_001143985.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143985.1

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BANF1 ( NM_003860.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003851.1 Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15955
Clone ID Related Accession (Same CDS sequence)	NM_001143985.1 , NM_003860.3 , XM_017018515.1 , XM_017018515.2 , NM_003860.4 , XM_017018514.1
Accession Version	NM_003860.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	barrier-to-autointegration factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF068235.1, BG748852.1, BC005942.1 and BC008705.1. This sequence is a reference standard in the RefSeqGene project. On Jan 24, 2009 this sequence version replaced NM_003860.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF068235.1, SRR1163655.82109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003851.1
CDS	509..778
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	NM_003860.3
Organism	Homo sapiens(human)
Definition	Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003860.3

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BANF1 ( NM_003860.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003851.1 Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15955
Clone ID Related Accession (Same CDS sequence)	NM_001143985.1 , NM_003860.3 , XM_017018515.1 , XM_017018515.2 , NM_003860.4 , XM_017018514.1
Accession Version	NM_003860.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	barrier-to-autointegration factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005942.1, BC008705.1 and AP006287.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_003860.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.263062.1, SRR1163658.467813.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312175.7/ ENSP00000310275.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003851.1
CDS	85..354
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	NM_003860.4
Organism	Homo sapiens(human)
Definition	Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003860.4

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BANF1 ( XM_017018515.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874004.1 Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu15955
Clone ID Related Accession (Same CDS sequence)	NM_001143985.1 , NM_003860.3 , XM_017018515.1 , XM_017018515.2 , NM_003860.4 , XM_017018514.1
Accession Version	XM_017018515.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	barrier-to-autointegration factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874004.1
CDS	42..311
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	XM_017018515.1
Organism	Homo sapiens(human)
Definition	Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018515.1

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BANF1 ( XM_017018515.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874004.1 Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu15955
Clone ID Related Accession (Same CDS sequence)	NM_001143985.1 , NM_003860.3 , XM_017018515.1 , XM_017018515.2 , NM_003860.4 , XM_017018514.1
Accession Version	XM_017018515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	barrier-to-autointegration factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874004.1
CDS	59..328
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	XM_017018515.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018515.2

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BANF1 ( XM_017018514.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874003.1 Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu15955
Clone ID Related Accession (Same CDS sequence)	NM_001143985.1 , NM_003860.3 , XM_017018515.1 , XM_017018515.2 , NM_003860.4 , XM_017018514.1
Accession Version	XM_017018514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	barrier-to-autointegration factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874003.1
CDS	502..771
Translation	MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Target ORF information:

RefSeq Version	XM_017018514.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BAF nuclear assembly factor 1 (BANF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018514.1

ORF Insert Sequence:

ATGACAACCT CCCAAAAGCA CCGAGACTTC GTGGCAGAGC CCATGGGGGA GAAGCCAGTG 
GGGAGCCTGG CTGGGATTGG TGAAGTCCTG GGCAAGAAGC TGGAGGAAAG GGGTTTTGAC 
AAGGCCTATG TTGTCCTTGG CCAGTTTCTG GTGCTAAAGA AAGATGAAGA CCTCTTCCGG 
GAATGGCTGA AAGACACTTG TGGCGCCAAC GCCAAGCAGT CCCGGGACTG CTTCGGATGC 
CTTCGAGAGT GGTGCGACGC CTTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LEM-domain proteins are lost during human spermiogenesis but BAF and BAF-L persist.
Reproduction (Cambridge, England)154(4)387-401(2017 Oct)
Elkhatib RA,Paci M,Boissier R,Longepied G,Auguste Y,Achard V,Bourgeois P,Levy N,Branger N,Mitchell MJ,Metzler-Guillemain C

VRK2A is an A-type lamin-dependent nuclear envelope kinase that phosphorylates BAF.
Molecular biology of the cell28(17)2241-2250(2017 Aug)
Birendra Kc,May DG,Benson BV,Kim DI,Shivega WG,Ali MH,Faustino RS,Campos AR,Roux KJ

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BANF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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