FPGT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FPGT
Entrez Gene ID	8790
Full Name	fucose-1-phosphate guanylyltransferase
Synonyms	GFPP
General protein information	Preferred Names fucose-1-phosphate guanylyltransferase Names fucose-1-phosphate guanylyltransferase GDP-L-fucose diphosphorylase GDP-beta-L-fucose pyrophosphorylase fucose-1-phosphate guanyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p31.1
Summary	L-fucose is a key sugar in glycoproteins and other complex carbohydrates since it may be involved in many of the functional roles of these macromolecules, such as in cell-cell recognition. The fucosyl donor for these fucosylated oligosaccharides is GDP-beta-L-fucose. There are two alternate pathways for the biosynthesis of GDP-fucose; the major pathway converts GDP-alpha-D-mannose to GDP-beta-L-fucose. The protein encoded by this gene participates in an alternate pathway that is present in certain mammalian tissues, such as liver and kidney, and appears to function as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. This pathway involves the phosphorylation of L-fucose to form beta-L-fucose-1-phosphate, and then condensation of the beta-L-fucose-1-phosphate with GTP by fucose-1-phosphate guanylyltransferase to form GDP-beta-L-fucose. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream TNNI3 interacting kinase (TNNI3K) gene. [provided by RefSeq, Dec 2010].
Disorder MIM:	603609

mRNA and Protein(s)

mRNA	Protein	Name
NM_003838.4	NP_003829.3	fucose-1-phosphate guanylyltransferase isoform 1
NM_001199329.2	NP_001186258.2	fucose-1-phosphate guanylyltransferase isoform 2
NM_001199328.2	NP_001186257.2	fucose-1-phosphate guanylyltransferase isoform 3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	FPGT	NP_001191744.1
Canis lupus familiaris (dog)	FPGT	NP_001191739.1
Mus musculus (house mouse)	Fpgt	NP_083606.2
Pan troglodytes (chimpanzee)	FPGT	NP_001191725.1
Caenorhabditis elegans (roundworm)	CELE_K03H1.13	NP_001255051.1
Bos taurus (cattle)	FPGT	NP_001178212.1
Danio rerio (zebrafish)	fpgt	NP_001018590.2
Xenopus tropicalis (tropical clawed frog)	fpgt	NP_001191751.1
Homo sapiens (human)	FPGT	NP_003829.3
Rattus norvegicus (Norway rat)	Fpgt	NP_955788.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Purification, crystallization and preliminary X-ray characterization of the human GTP fucose pyrophosphorylase.
Quirk S, Seley-Radtke KL
Acta crystallographica. Section F, Structural biology and crystallization communications62(Pt 4)392-4(2006 Apr)

Identification of catalytic amino acids in the human GTP fucose pyrophosphorylase active site.
Quirk S, Seley KL
Biochemistry44(39)13172-8(2005 Oct)

Substrate discrimination by the human GTP fucose pyrophosphorylase.
Quirk S, Seley KL
Biochemistry44(32)10854-63(2005 Aug)

GDP-L-fucose pyrophosphorylase. Purification, cDNA cloning, and properties of the enzyme.
Pastuszak I, Ketchum C, Hermanson G, Sjoberg EJ, Drake R, Elbein AD
The Journal of biological chemistry273(46)30165-74(1998 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A complete native data set has been collected as a first step in determining the three-dimensional structure of this enzyme.
Title: Purification, crystallization and preliminary X-ray characterization of the human GTP fucose pyrophosphorylase.

identified five amino acid residues that are critical for catalysis.
Title: Identification of catalytic amino acids in the human GTP fucose pyrophosphorylase active site.

the nature of the purine base is the major determinant in substrate specificity, followed by the nature of the hexose-1-P, and finally by the ribose moiety. Binding is enthalpy-driven and does not involve proton transfer
Title: Substrate discrimination by the human GTP fucose pyrophosphorylase.

The following FPGT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FPGT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01361	NM_003838.4 Latest version!	Homo sapiens fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu01310	NM_001199329.2 Latest version!	Homo sapiens fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu01479	NM_001199328.2 Latest version!	Homo sapiens fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FPGT ( NM_003838.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003829.3 Homo sapiens fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01361
Clone ID Related Accession (Same CDS sequence)	NM_003838.4
Accession Version	NM_003838.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	fucose-1-phosphate guanylyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB066016.1, AF017445.1 and AC098692.3. On Jun 5, 2013 this sequence version replaced NM_003838.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: DB066016.1, SRR1660809.78047.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGTGCTG TGCGGCGCGG TCTCAGGGAA GGTGGGGCTA TGGCAGCTGC TAGGGACCCT 
CCGGAAGTAT CGCTGCGAGA AGCCACCCAG CGAAAATTGC GGAGGTTTTC CGAGCTAAGA 
GGCAAACTTG TAGCACGTGG AGAATTCTGG GACATAGTTG CAATAACAGC GGCTGATGAA 
AAACAGGAAC TTGCTTACAA CCAACAGCTG TCAGAAAAGC TGAAAAGAAA GGAGTTACCC 
CTTGGAGTTC AATATCACGT TTTTGTGGAT CCTGCTGGAG CCAAAATTGG AAATGGAGGA 
TCAACACTTT GTGCCCTTCA ATGTTTGGAA AAGCTATATG GAGATAAATG GAATTCTTTT 
ACCATCTTAT TAATTCACTC TGGTGGCTAC AGTCAACGAC TTCCAAATGC AAGTGCTCTG 
GGAAAAATTT TCACTGCTTT ACCTCTTGGT AACCCCATTT ATCAGATGCT AGAATTAAAA 
CTAGCCATGT ACATTGATTT CCCCTTAAAT ATGAATCCTG GAATTCTGGT TACCTGTGCA 
GATGATATTG AACTTTATAG TATTGGAGAA TTTGAGTTTA TTAGGTTTGA CAAACCTGGC 
TTTACTGCTT TAGCTCATCC TTCTAGTTTG ACGATAGGTA CCACACATGG AGTATTTGTC 
TTAGATCCTT TTGATGATTT AAAACATAGA GACCTTGAAT ACAGGTCTTG CCATCGTTTC 
CTTCATAAGC CCAGCATAGA AAAGATGTAT CAGTTTAATG CTGTGTGTAG ACCTGGAAAT 
TTTTGTCAAC AGGACTTTGC TGGGGGTGAC ATTGCCGATC TTAAATTAGA CTCTGACTAT 
GTCTACACAG ATAGCCTATT TTATATGGAT CATAAATCAG CAAAAATGTT ACTTGCTTTT 
TATGAAAAAA TAGGCACACT GAGCTGTGAA ATAGATGCCT ATGGTGACTT TCTGCAGGCT 
TTGGGACCTG GAGCAACTGT GGAGTACACC AGAAACACAT CAAATGTCAT TAAAGAAGAG 
TCAGAGTTGG TAGAAATGAG GCAGAGAATA TTTCATCTTC TTAAAGGAAC ATCACTAAAT 
GTTGTTGTTC TTAATAACTC CAAATTTTAT CACATTGGAA CAACCGAAGA ATATTTGTTT 
TACTTTACCT CAGATAACAG TTTAAAGTCA GAGCTCGGCT TACAGTCCAT AACTTTTAGT 
ATCTTTCCAG ATATACCAGA ATGCTCTGGC AAAACATCCT GTATCATTCA AAGCATACTG 
GATTCAAGAT GTTCTGTGGC ACCTGGCTCA GTTGTGGAGT ATTCCAGATT GGGGCCTGAT 
GTTTCAGTTG GGGAAAACTG CATTATTAGT GGTTCTTACA TCCTAACAAA AGCTGCCCTC 
CCCGCACATT CTTTTGTATG TTCCTTAAGC TTAAAGATGA ATAGATGCTT AAAGTATGCA 
ACTATGGCAT TTGGAGTGCA AGACAACTTG AAAAAGAGTG TGAAAACATT GTCAGATATA 
AAGTTACTTC AATTCTTTGG AGTCTGTTTC CTGTCATGCT TAGATGTTTG GAATCTTAAA 
GTTACAGAGG AACTGTTCTC TGGTAACAAG ACATGTCTGA GTTTGTGGAC TGCACGCATT 
TTCCCAGTTT GTTCTTCTTT GAGTGACTCA GTTATAACAT CCCTAAAGAT GTTAAATGCT 
GTTAAGAACA AGTCAGCATT CAGCCTGAAT AGCTATAAGT TGCTGTCCAT TGAAGAAATG 
CTTATCTACA AAGATGTAGA AGATATGATA ACTTACAGGG AACAAATTTT TCTAGAAATC 
AGTTTAAAAA GCAGTTTGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003829.3
CDS	29..1852
Translation	MRAVRRGLREGGAMAAARDPPEVSLREATQRKLRRFSELRGKLVARGEFWDIVAITAADEKQELAYNQQLSEKLKRKELPLGVQYHVFVDPAGAKIGNGGSTLCALQCLEKLYGDKWNSFTILLIHSGGYSQRLPNASALGKIFTALPLGNPIYQMLELKLAMYIDFPLNMNPGILVTCADDIELYSIGEFEFIRFDKPGFTALAHPSSLTIGTTHGVFVLDPFDDLKHRDLEYRSCHRFLHKPSIEKMYQFNAVCRPGNFCQQDFAGGDIADLKLDSDYVYTDSLFYMDHKSAKMLLAFYEKIGTLSCEIDAYGDFLQALGPGATVEYTRNTSNVIKEESELVEMRQRIFHLLKGTSLNVVVLNNSKFYHIGTTEEYLFYFTSDNSLKSELGLQSITFSIFPDIPECSGKTSCIIQSILDSRCSVAPGSVVEYSRLGPDVSVGENCIISGSYILTKAALPAHSFVCSLSLKMNRCLKYATMAFGVQDNLKKSVKTLSDIKLLQFFGVCFLSCLDVWNLKVTEELFSGNKTCLSLWTARIFPVCSSLSDSVITSLKMLNAVKNKSAFSLNSYKLLSIEEMLIYKDVEDMITYREQIFLEISLKSSLM

Target ORF information:

RefSeq Version	NM_003838.4
Organism	Homo sapiens(human)
Definition	Homo sapiens fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003838.4

ORF Insert Sequence:

ATGCGTGCTG TGCGGCGCGG TCTCAGGGAA GGTGGGGCTA TGGCAGCTGC TAGGGACCCT 
CCGGAAGTAT CGCTGCGAGA AGCCACCCAG CGAAAATTGC GGAGGTTTTC CGAGCTAAGA 
GGCAAACTTG TAGCACGTGG AGAATTCTGG GACATAGTTG CAATAACAGC GGCTGATGAA 
AAACAGGAAC TTGCTTACAA CCAACAGCTG TCAGAAAAGC TGAAAAGAAA GGAGTTACCC 
CTTGGAGTTC AATATCACGT TTTTGTGGAT CCTGCTGGAG CCAAAATTGG AAATGGAGGA 
TCAACACTTT GTGCCCTTCA ATGTTTGGAA AAGCTATATG GAGATAAATG GAATTCTTTT 
ACCATCTTAT TAATTCACTC TGGTGGCTAC AGTCAACGAC TTCCAAATGC AAGTGCTCTG 
GGAAAAATTT TCACTGCTTT ACCTCTTGGT AACCCCATTT ATCAGATGCT AGAATTAAAA 
CTAGCCATGT ACATTGATTT CCCCTTAAAT ATGAATCCTG GAATTCTGGT TACCTGTGCA 
GATGATATTG AACTTTATAG TATTGGAGAA TTTGAGTTTA TTAGGTTTGA CAAACCTGGC 
TTTACTGCTT TAGCTCATCC TTCTAGTTTG ACGATAGGTA CCACACATGG AGTATTTGTC 
TTAGATCCTT TTGATGATTT AAAACATAGA GACCTTGAAT ACAGGTCTTG CCATCGTTTC 
CTTCATAAGC CCAGCATAGA AAAGATGTAT CAGTTTAATG CTGTGTGTAG ACCTGGAAAT 
TTTTGTCAAC AGGACTTTGC TGGGGGTGAC ATTGCCGATC TTAAATTAGA CTCTGACTAT 
GTCTACACAG ATAGCCTATT TTATATGGAT CATAAATCAG CAAAAATGTT ACTTGCTTTT 
TATGAAAAAA TAGGCACACT GAGCTGTGAA ATAGATGCCT ATGGTGACTT TCTGCAGGCT 
TTGGGACCTG GAGCAACTGT GGAGTACACC AGAAACACAT CAAATGTCAT TAAAGAAGAG 
TCAGAGTTGG TAGAAATGAG GCAGAGAATA TTTCATCTTC TTAAAGGAAC ATCACTAAAT 
GTTGTTGTTC TTAATAACTC CAAATTTTAT CACATTGGAA CAACCGAAGA ATATTTGTTT 
TACTTTACCT CAGATAACAG TTTAAAGTCA GAGCTCGGCT TACAGTCCAT AACTTTTAGT 
ATCTTTCCAG ATATACCAGA ATGCTCTGGC AAAACATCCT GTATCATTCA AAGCATACTG 
GATTCAAGAT GTTCTGTGGC ACCTGGCTCA GTTGTGGAGT ATTCCAGATT GGGGCCTGAT 
GTTTCAGTTG GGGAAAACTG CATTATTAGT GGTTCTTACA TCCTAACAAA AGCTGCCCTC 
CCCGCACATT CTTTTGTATG TTCCTTAAGC TTAAAGATGA ATAGATGCTT AAAGTATGCA 
ACTATGGCAT TTGGAGTGCA AGACAACTTG AAAAAGAGTG TGAAAACATT GTCAGATATA 
AAGTTACTTC AATTCTTTGG AGTCTGTTTC CTGTCATGCT TAGATGTTTG GAATCTTAAA 
GTTACAGAGG AACTGTTCTC TGGTAACAAG ACATGTCTGA GTTTGTGGAC TGCACGCATT 
TTCCCAGTTT GTTCTTCTTT GAGTGACTCA GTTATAACAT CCCTAAAGAT GTTAAATGCT 
GTTAAGAACA AGTCAGCATT CAGCCTGAAT AGCTATAAGT TGCTGTCCAT TGAAGAAATG 
CTTATCTACA AAGATGTAGA AGATATGATA ACTTACAGGG AACAAATTTT TCTAGAAATC 
AGTTTAAAAA GCAGTTTGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FPGT ( NM_001199329.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186258.2 Homo sapiens fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01310
Clone ID Related Accession (Same CDS sequence)	NM_001199329.2
Accession Version	NM_001199329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	fucose-1-phosphate guanylyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB066016.1, AK304490.1 and AC098692.3. On Jun 5, 2013 this sequence version replaced NM_001199329.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK304490.1, KU178440.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTGCTG TGCGGCGCGG TCTCAGGGAA GGTGGGGCTA TGGCAGCTGC TAGGGACCCT 
CCGGAAGTAT CGCTGCGAGA AGCCACCCAG CGAAAATTGC GGAGGTTTTC CGAGCTAAGA 
GGCAAACTTG TAGCACGTGG AGAATTCTGG GACATAGTTG CAATAACAGC GGCTGATGAA 
AAACAGGAAC TTGCTTACAA CCAACAGCTG TCAGAAAAGC TGAAAAGAAA GGAGTTACCC 
CTTGGAGTTC AATATCACGT TTTTGTGGAT CCTGCTGGAG CCAAAATTGG AAATGGAGGA 
TCAACACTTT GTGCCCTTCA ATGTTTGGAA AAGCTATATG GAGATAAATG GAATTCTTTT 
ACCATCTTAT TAATTCACTC TGTATCTTTC CAGATATACC AGAATGCTCT GGCAAAACAT 
CCTGTATCAT TCAAAGCATA CTGGATTCAA GATGTTCTGT GGCACCTGGC TCAGTTGTGG 
AGTATTCCAG ATTGGGGCCT GATGTTTCAG TTGGGGAAAA CTGCATTATT AGTGGTTCTT 
ACATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186258.2
CDS	29..577
Translation	MRAVRRGLREGGAMAAARDPPEVSLREATQRKLRRFSELRGKLVARGEFWDIVAITAADEKQELAYNQQLSEKLKRKELPLGVQYHVFVDPAGAKIGNGGSTLCALQCLEKLYGDKWNSFTILLIHSVSFQIYQNALAKHPVSFKAYWIQDVLWHLAQLWSIPDWGLMFQLGKTALLVVLTS

Target ORF information:

RefSeq Version	NM_001199329.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199329.2

ORF Insert Sequence:

ATGCGTGCTG TGCGGCGCGG TCTCAGGGAA GGTGGGGCTA TGGCAGCTGC TAGGGACCCT 
CCGGAAGTAT CGCTGCGAGA AGCCACCCAG CGAAAATTGC GGAGGTTTTC CGAGCTAAGA 
GGCAAACTTG TAGCACGTGG AGAATTCTGG GACATAGTTG CAATAACAGC GGCTGATGAA 
AAACAGGAAC TTGCTTACAA CCAACAGCTG TCAGAAAAGC TGAAAAGAAA GGAGTTACCC 
CTTGGAGTTC AATATCACGT TTTTGTGGAT CCTGCTGGAG CCAAAATTGG AAATGGAGGA 
TCAACACTTT GTGCCCTTCA ATGTTTGGAA AAGCTATATG GAGATAAATG GAATTCTTTT 
ACCATCTTAT TAATTCACTC TGTATCTTTC CAGATATACC AGAATGCTCT GGCAAAACAT 
CCTGTATCAT TCAAAGCATA CTGGATTCAA GATGTTCTGT GGCACCTGGC TCAGTTGTGG 
AGTATTCCAG ATTGGGGCCT GATGTTTCAG TTGGGGAAAA CTGCATTATT AGTGGTTCTT 
ACATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FPGT ( NM_001199328.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186257.2 Homo sapiens fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01479
Clone ID Related Accession (Same CDS sequence)	NM_001199328.2
Accession Version	NM_001199328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	fucose-1-phosphate guanylyltransferase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB066016.1, AK299468.1 and AC098692.3. On Jun 5, 2013 this sequence version replaced NM_001199328.1. Transcript Variant: This variant (3) lacks a segment in the 3' coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.194242.1, AK299468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTGCTG TGCGGCGCGG TCTCAGGGAA GGTGGGGCTA TGGCAGCTGC TAGGGACCCT 
CCGGAAGTAT CGCTGCGAGA AGCCACCCAG CGAAAATTGC GGAGGTTTTC CGAGCTAAGA 
GGCAAACTTG TAGCACGTGG AGAATTCTGG GACATAGTTG CAATAACAGC GGCTGATGAA 
AAACAGGAAC TTGCTTACAA CCAACAGCTG TCAGAAAAGC TGAAAAGAAA GGAGTTACCC 
CTTGGAGTTC AATATCACGT TTTTGTGGAT CCTGCTGGAG CCAAAATTGG AAATGGAGGA 
TCAACACTTT GTGCCCTTCA ATGTTTGGAA AAGCTATATG GAGATAAATG GAATTCTTTT 
ACCATCTTAT TAATTCACTC TGGTGGCTAC AGTCAACGAC TTCCAAATGC AAGTGCTCTG 
GGAAAAATTT TCACTGCTTT ACCTCTTGAT ATACCAGAAT GCTCTGGCAA AACATCCTGT 
ATCATTCAAA GCATACTGGA TTCAAGATGT TCTGTGGCAC CTGGCTCAGT TGTGGAGTAT 
TCCAGATTGG GGCCTGATGT TTCAGTTGGG GAAAACTGCA TTATTAGTGG TTCTTACATC 
CTAACAAAAG CTGCCCTCCC CGCACATTCT TTTGTATGTT CCTTAAGCTT AAAGATGAAT 
AGATGCTTAA AGTATGCAAC TATGGCATTT GGAGTGCAAG ACAACTTGAA AAAGAGTGTG 
AAAACATTGT CAGATATAAA GTTACTTCAA TTCTTTGGAG TCTGTTTCCT GTCATGCTTA 
GATGTTTGGA ATCTTAAAGT TACAGAGGAA CTGTTCTCTG GTAACAAGAC ATGTCTGAGT 
TTGTGGACTG CACGCATTTT CCCAGTTTGT TCTTCTTTGA GTGACTCAGT TATAACATCC 
CTAAAGATGT TAAATGCTGT TAAGAACAAG TCAGCATTCA GCCTGAATAG CTATAAGTTG 
CTGTCCATTG AAGAAATGCT TATCTACAAA GATGTAGAAG ATATGATAAC TTACAGGGAA 
CAAATTTTTC TAGAAATCAG TTTAAAAAGC AGTTTGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186257.2
CDS	29..1090
Translation	MRAVRRGLREGGAMAAARDPPEVSLREATQRKLRRFSELRGKLVARGEFWDIVAITAADEKQELAYNQQLSEKLKRKELPLGVQYHVFVDPAGAKIGNGGSTLCALQCLEKLYGDKWNSFTILLIHSGGYSQRLPNASALGKIFTALPLDIPECSGKTSCIIQSILDSRCSVAPGSVVEYSRLGPDVSVGENCIISGSYILTKAALPAHSFVCSLSLKMNRCLKYATMAFGVQDNLKKSVKTLSDIKLLQFFGVCFLSCLDVWNLKVTEELFSGNKTCLSLWTARIFPVCSSLSDSVITSLKMLNAVKNKSAFSLNSYKLLSIEEMLIYKDVEDMITYREQIFLEISLKSSLM

Target ORF information:

RefSeq Version	NM_001199328.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fucose-1-phosphate guanylyltransferase (FPGT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199328.2

ORF Insert Sequence:

ATGCGTGCTG TGCGGCGCGG TCTCAGGGAA GGTGGGGCTA TGGCAGCTGC TAGGGACCCT 
CCGGAAGTAT CGCTGCGAGA AGCCACCCAG CGAAAATTGC GGAGGTTTTC CGAGCTAAGA 
GGCAAACTTG TAGCACGTGG AGAATTCTGG GACATAGTTG CAATAACAGC GGCTGATGAA 
AAACAGGAAC TTGCTTACAA CCAACAGCTG TCAGAAAAGC TGAAAAGAAA GGAGTTACCC 
CTTGGAGTTC AATATCACGT TTTTGTGGAT CCTGCTGGAG CCAAAATTGG AAATGGAGGA 
TCAACACTTT GTGCCCTTCA ATGTTTGGAA AAGCTATATG GAGATAAATG GAATTCTTTT 
ACCATCTTAT TAATTCACTC TGGTGGCTAC AGTCAACGAC TTCCAAATGC AAGTGCTCTG 
GGAAAAATTT TCACTGCTTT ACCTCTTGAT ATACCAGAAT GCTCTGGCAA AACATCCTGT 
ATCATTCAAA GCATACTGGA TTCAAGATGT TCTGTGGCAC CTGGCTCAGT TGTGGAGTAT 
TCCAGATTGG GGCCTGATGT TTCAGTTGGG GAAAACTGCA TTATTAGTGG TTCTTACATC 
CTAACAAAAG CTGCCCTCCC CGCACATTCT TTTGTATGTT CCTTAAGCTT AAAGATGAAT 
AGATGCTTAA AGTATGCAAC TATGGCATTT GGAGTGCAAG ACAACTTGAA AAAGAGTGTG 
AAAACATTGT CAGATATAAA GTTACTTCAA TTCTTTGGAG TCTGTTTCCT GTCATGCTTA 
GATGTTTGGA ATCTTAAAGT TACAGAGGAA CTGTTCTCTG GTAACAAGAC ATGTCTGAGT 
TTGTGGACTG CACGCATTTT CCCAGTTTGT TCTTCTTTGA GTGACTCAGT TATAACATCC 
CTAAAGATGT TAAATGCTGT TAAGAACAAG TCAGCATTCA GCCTGAATAG CTATAAGTTG 
CTGTCCATTG AAGAAATGCT TATCTACAAA GATGTAGAAG ATATGATAAC TTACAGGGAA 
CAAATTTTTC TAGAAATCAG TTTAAAAAGC AGTTTGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Purification, crystallization and preliminary X-ray characterization of the human GTP fucose pyrophosphorylase.
Acta crystallographica. Section F, Structural biology and crystallization communications62(Pt 4)392-4(2006 Apr)
Quirk S,Seley-Radtke KL

Identification of catalytic amino acids in the human GTP fucose pyrophosphorylase active site.
Biochemistry44(39)13172-8(2005 Oct)
Quirk S,Seley KL

Substrate discrimination by the human GTP fucose pyrophosphorylase.
Biochemistry44(32)10854-63(2005 Aug)
Quirk S,Seley KL

GDP-L-fucose pyrophosphorylase. Purification, cDNA cloning, and properties of the enzyme.
The Journal of biological chemistry273(46)30165-74(1998 Nov)
Pastuszak I,Ketchum C,Hermanson G,Sjoberg EJ,Drake R,Elbein AD

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FPGT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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