GPAA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPAA1
Entrez Gene ID	8733
Full Name	glycosylphosphatidylinositol anchor attachment 1
Synonyms	GAA1,GPIBD15,hGAA1
General protein information	Preferred Names glycosylphosphatidylinositol anchor attachment 1 Names glycosylphosphatidylinositol anchor attachment 1 protein GAA1 protein homolog GPAA1P anchor attachment protein 1 homolog GPI anchor attachment protein 1 GPI transamidase subunit anchor attachment protein 1 (Gaa1p, yeast) homolog glycophosphatidylinositol anchor attachment 1 glycosylphosphatidylinositol anchor attachment protein 1 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q24.3
Summary	Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq, Jul 2008].
Disorder MIM:	603048
Disorder Html:	Glycosylphosphatidylinositol biosynthesis defect 15, 617810 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003801.3	NP_003792.1	glycosylphosphatidylinositol anchor attachment 1 protein
NM_003801.4	NP_003792.1	glycosylphosphatidylinositol anchor attachment 1 protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPAA1	XP_001155503.1
Magnaporthe oryzae (rice blast fungus)	MGG_03523	XP_003716396.1
Xenopus tropicalis (tropical clawed frog)	gpaa1	NP_001015809.1
Rattus norvegicus (Norway rat)	Gpaa1	NP_001004240.1
Danio rerio (zebrafish)	LOC555496	XP_005171175.1
Drosophila melanogaster (fruit fly)	CG3033	NP_572273.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010738	XP_311451.4
Schizosaccharomyces pombe (fission yeast)	gaa1	NP_593498.1
Neurospora crassa	NCU02368	XP_959789.1
Mus musculus (house mouse)	Gpaa1	NP_034461.1
Homo sapiens (human)	GPAA1	NP_003792.1
Canis lupus familiaris (dog)	GPAA1	XP_532350.3
Kluyveromyces lactis	KLLA0F19118g	XP_455938.1
Eremothecium gossypii	AGOS_AGR087C	NP_986752.1
	GPAA1	XP_001088872.1
Bos taurus (cattle)	GPAA1	NP_001019700.1
Saccharomyces cerevisiae (baker's yeast)	GAA1	NP_013189.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in GPAA1, Encoding a GPI Transamidase Complex Protein, Cause Developmental Delay, Epilepsy, Cerebellar Atrophy, and Osteopenia.
Nguyen TTM, Murakami Y, Sheridan E, Ehresmann S, Rousseau J, St-Denis A, Chai G, Ajeawung NF, Fairbrother L, Reimschisel T, Bateman A, Berry-Kravis E, Xia F, Tardif J, Parry DA, Logan CV, Diggle C, Bennett CP, Hattingh L, Rosenfeld JA, Perry MS, Parker MJ, Le Deist F, Zaki MS, Ignatius E, Isohanni P, L?nnqvist T, Carroll CJ, Johnson CA, Gleeson JG, Kinoshita T, Campeau PM
American journal of human genetics101(5)856-865(2017 Nov)

Transamidase subunit GAA1/GPAA1 is a M28 family metallo-peptide-synthetase that catalyzes the peptide bond formation between the substrate protein's omega-site and the GPI lipid anchor's phosphoethanolamine.
Eisenhaber B, Eisenhaber S, Kwang TY, Gr?ber G, Eisenhaber F
Cell cycle (Georgetown, Tex.)13(12)1912-7(2014)

Human metabolic individuality in biomedical and pharmaceutical research.
Suhre K, Shin SY, Petersen AK, Mohney RP, Meredith D, W?gele B, Altmaier E, , Deloukas P, Erdmann J, Grundberg E, Hammond CJ, de Angelis MH, Kastenm?ller G, K?ttgen A, Kronenberg F, Mangino M, Meisinger C, Meitinger T, Mewes HW, Milburn MV, Prehn C, Raffler J, Ried JS, R?misch-Margl W, Samani NJ, Small KS, Wichmann HE, Zhai G, Illig T, Spector TD, Adamski J, Soranzo N, Gieger C
Nature477(7362)54-60(2011 Aug)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The splicing mutation was found to decrease GPAA1 mRNA.
Title: Mutations in GPAA1, Encoding a GPI Transamidase Complex Protein, Cause Developmental Delay, Epilepsy, Cerebellar Atrophy, and Osteopenia.

the lumenal domain of GAA1/GPAA1 has a 3D structure similar to that of an M28-type aminopeptidase. GAA1/GPAA1 is a candidate for the enzyme that catalyzes the peptide bond formation between the omega-site and a phosphoethanolamine group of GPI lipid anchor.
Title: Transamidase subunit GAA1/GPAA1 is a M28 family metallo-peptide-synthetase that catalyzes the peptide bond formation between the substrate protein's omega-site and the GPI lipid anchor's phosphoethanolamine.

Results show an increased expression level and elevated copy number for GAA1 in head and neck squamous carcinoma, suggesting a role for this GPI anchor subunit in HNSCC.
Title: Alterations of GPI transamidase subunits in head and neck squamous carcinoma.

Increased expression of glycosyl-phosphatidylinositol anchor attachment protein 1 is associated with gene amplification in hepatocellular carcinoma
Title: Increased expression of glycosyl-phosphatidylinositol anchor attachment protein 1 (GPAA1) is associated with gene amplification in hepatocellular carcinoma.

passively retained in the ER by a signalless mechanism
Title: Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex.

The following GPAA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPAA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30453	NM_003801.4 Latest version!	Homo sapiens glycosylphosphatidylinositol anchor attachment 1 (GPAA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
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OHu30453	NM_003801.3	Homo sapiens glycosylphosphatidylinositol anchor attachment 1 (GPAA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPAA1 ( NM_003801.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003792.1 Homo sapiens glycosylphosphatidylinositol anchor attachment 1 (GPAA1), mRNA.

Price & Availability↑

CloneID	OHu30453
Clone ID Related Accession (Same CDS sequence)	NM_003801.3 , NM_003801.4
Accession Version	NM_003801.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	glycosylphosphatidylinositol anchor attachment 1 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC004129.2 and BC003171.1. On Dec 8, 2005 this sequence version replaced NM_003801.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004129.2, SRR1163657.316279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCCTCC TGTCGGACCC GGTTCGCCGG CGCGCGCTCG CCCGCCTAGT GCTGCGCCTC 
AACGCGCCGT TGTGCGTGCT GAGCTACGTG GCGGGCATCG CCTGGTTCTT GGCGCTGGTT 
TTCCCGCCGC TGACCCAGCG CACTTACATG TCGGAGAACG CCATGGGCTC CACCATGGTG 
GAGGAGCAGT TTGCGGGCGG AGACCGTGCC CGGGCTTTTG CCCGGGACTT CGCCGCCCAC 
CGCAAGAAGT CGGGGGCTCT GCCAGTGGCC TGGCTTGAAC GGACGATGCG GTCAGTAGGG 
CTGGAGGTCT ACACGCAGAG TTTCTCCCGG AAACTGCCCT TCCCAGATGA GACCCACGAG 
CGCTATATGG TGTCGGGCAC CAACGTGTAC GGCATCCTGC GGGCCCCGCG TGCTGCCAGC 
ACCGAGTCGC TTGTGCTCAC CGTGCCCTGT GGCTCTGACT CTACCAACAG CCAGGCTGTG 
GGGCTGCTGC TGGCACTGGC TGCCCACTTC CGGGGGCAGA TTTATTGGGC CAAAGATATC 
GTCTTCCTGG TAACAGAACA TGACCTTCTG GGCACTGAGG CTTGGCTTGA AGCCTACCAC 
GATGTCAATG TCACTGGCAT GCAGTCGTCT CCCCTGCAGG GCCGAGCTGG GGCCATTCAG 
GCAGCCGTGG CCCTGGAGCT GAGCAGTGAT GTGGTCACCA GCCTCGATGT GGCCGTGGAG 
GGGCTTAACG GGCAGCTGCC CAACCTTGAC CTGCTCAATC TCTTCCAGAC CTTCTGCCAG 
AAAGGGGGCC TGTTGTGCAC GCTTCAGGGC AAGCTGCAGC CCGAGGACTG GACATCATTG 
GATGGACCGC TGCAGGGCCT GCAGACACTG CTGCTCATGG TTCTGCGGCA GGCCTCCGGC 
CGCCCCCACG GCTCCCATGG CCTCTTCCTG CGCTACCGTG TGGAGGCCCT AACCCTGCGT 
GGCATCAATA GCTTCCGCCA GTACAAGTAT GACCTGGTGG CAGTGGGCAA GGCTTTGGAG 
GGCATGTTCC GCAAGCTCAA CCACCTCCTG GAGCGCCTGC ACCAGTCCTT CTTCCTCTAC 
TTGCTCCCCG GCCTCTCCCG CTTCGTCTCC ATCGGCCTCT ACATGCCCGC TGTCGGCTTC 
TTGCTCCTGG TCCTTGGTCT CAAGGCTCTG GAACTGTGGA TGCAGCTGCA TGAGGCTGGA 
ATGGGCCTTG AGGAGCCCGG GGGTGCCCCT GGCCCCAGTG TACCCCTTCC CCCATCACAG 
GGTGTGGGGC TGGCCTCGCT CGTGGCACCT CTGCTGATCT CACAGGCCAT GGGACTGGCC 
CTCTATGTCC TGCCAGTGCT GGGCCAACAC GTTGCCACCC AGCACTTCCC AGTGGCAGAG 
GCTGAGGCTG TGGTGCTGAC ACTGCTGGCG ATTTATGCAG CTGGCCTGGC CCTGCCCCAC 
AATACCCACC GGGTGGTAAG CACACAGGCC CCAGACAGGG GCTGGATGGC ACTGAAGCTG 
GTAGCCCTGA TCTACCTAGC ACTGCAGCTG GGCTGCATCG CCCTCACCAA CTTCTCACTG 
GGCTTCCTGC TGGCCACCAC CATGGTGCCC ACTGCTGCGC TTGCCAAGCC TCATGGGCCC 
CGGACCCTCT ATGCTGCCCT GCTGGTGCTG ACCAGCCCGG CAGCCACGCT CCTTGGCAGC 
CTGTTCCTGT GGCGGGAGCT GCAGGAGGCG CCACTGTCAC TGGCCGAGGG CTGGCAGCTC 
TTCCTGGCAG CGCTAGCCCA GGGTGTGCTG GAGCACCACA CCTACGGCGC CCTGCTCTTC 
CCACTGCTGT CCCTGGGCCT CTACCCCTGC TGGCTGCTTT TCTGGAATGT GCTCTTCTGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003792.1
CDS	111..1976
Translation	MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMVEEQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLFWK

Target ORF information:

RefSeq Version	NM_003801.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycosylphosphatidylinositol anchor attachment 1 (GPAA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003801.3

ORF Insert Sequence:

ATGGGCCTCC TGTCGGACCC GGTTCGCCGG CGCGCGCTCG CCCGCCTAGT GCTGCGCCTC 
AACGCGCCGT TGTGCGTGCT GAGCTACGTG GCGGGCATCG CCTGGTTCTT GGCGCTGGTT 
TTCCCGCCGC TGACCCAGCG CACTTACATG TCGGAGAACG CCATGGGCTC CACCATGGTG 
GAGGAGCAGT TTGCGGGCGG AGACCGTGCC CGGGCTTTTG CCCGGGACTT CGCCGCCCAC 
CGCAAGAAGT CGGGGGCTCT GCCAGTGGCC TGGCTTGAAC GGACGATGCG GTCAGTAGGG 
CTGGAGGTCT ACACGCAGAG TTTCTCCCGG AAACTGCCCT TCCCAGATGA GACCCACGAG 
CGCTATATGG TGTCGGGCAC CAACGTGTAC GGCATCCTGC GGGCCCCGCG TGCTGCCAGC 
ACCGAGTCGC TTGTGCTCAC CGTGCCCTGT GGCTCTGACT CTACCAACAG CCAGGCTGTG 
GGGCTGCTGC TGGCACTGGC TGCCCACTTC CGGGGGCAGA TTTATTGGGC CAAAGATATC 
GTCTTCCTGG TAACAGAACA TGACCTTCTG GGCACTGAGG CTTGGCTTGA AGCCTACCAC 
GATGTCAATG TCACTGGCAT GCAGTCGTCT CCCCTGCAGG GCCGAGCTGG GGCCATTCAG 
GCAGCCGTGG CCCTGGAGCT GAGCAGTGAT GTGGTCACCA GCCTCGATGT GGCCGTGGAG 
GGGCTTAACG GGCAGCTGCC CAACCTTGAC CTGCTCAATC TCTTCCAGAC CTTCTGCCAG 
AAAGGGGGCC TGTTGTGCAC GCTTCAGGGC AAGCTGCAGC CCGAGGACTG GACATCATTG 
GATGGACCGC TGCAGGGCCT GCAGACACTG CTGCTCATGG TTCTGCGGCA GGCCTCCGGC 
CGCCCCCACG GCTCCCATGG CCTCTTCCTG CGCTACCGTG TGGAGGCCCT AACCCTGCGT 
GGCATCAATA GCTTCCGCCA GTACAAGTAT GACCTGGTGG CAGTGGGCAA GGCTTTGGAG 
GGCATGTTCC GCAAGCTCAA CCACCTCCTG GAGCGCCTGC ACCAGTCCTT CTTCCTCTAC 
TTGCTCCCCG GCCTCTCCCG CTTCGTCTCC ATCGGCCTCT ACATGCCCGC TGTCGGCTTC 
TTGCTCCTGG TCCTTGGTCT CAAGGCTCTG GAACTGTGGA TGCAGCTGCA TGAGGCTGGA 
ATGGGCCTTG AGGAGCCCGG GGGTGCCCCT GGCCCCAGTG TACCCCTTCC CCCATCACAG 
GGTGTGGGGC TGGCCTCGCT CGTGGCACCT CTGCTGATCT CACAGGCCAT GGGACTGGCC 
CTCTATGTCC TGCCAGTGCT GGGCCAACAC GTTGCCACCC AGCACTTCCC AGTGGCAGAG 
GCTGAGGCTG TGGTGCTGAC ACTGCTGGCG ATTTATGCAG CTGGCCTGGC CCTGCCCCAC 
AATACCCACC GGGTGGTAAG CACACAGGCC CCAGACAGGG GCTGGATGGC ACTGAAGCTG 
GTAGCCCTGA TCTACCTAGC ACTGCAGCTG GGCTGCATCG CCCTCACCAA CTTCTCACTG 
GGCTTCCTGC TGGCCACCAC CATGGTGCCC ACTGCTGCGC TTGCCAAGCC TCATGGGCCC 
CGGACCCTCT ATGCTGCCCT GCTGGTGCTG ACCAGCCCGG CAGCCACGCT CCTTGGCAGC 
CTGTTCCTGT GGCGGGAGCT GCAGGAGGCG CCACTGTCAC TGGCCGAGGG CTGGCAGCTC 
TTCCTGGCAG CGCTAGCCCA GGGTGTGCTG GAGCACCACA CCTACGGCGC CCTGCTCTTC 
CCACTGCTGT CCCTGGGCCT CTACCCCTGC TGGCTGCTTT TCTGGAATGT GCTCTTCTGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPAA1 ( NM_003801.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003792.1 Homo sapiens glycosylphosphatidylinositol anchor attachment 1 (GPAA1), mRNA.

Price & Availability↑

CloneID	OHu30453
Clone ID Related Accession (Same CDS sequence)	NM_003801.3 , NM_003801.4
Accession Version	NM_003801.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	glycosylphosphatidylinositol anchor attachment 1 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC004129.2 and BC003171.1. On Nov 22, 2018 this sequence version replaced NM_003801.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.301001.1, SRR3476690.350575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355091.9/ ENSP00000347206.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCCTCC TGTCGGACCC GGTTCGCCGG CGCGCGCTCG CCCGCCTAGT GCTGCGCCTC 
AACGCGCCGT TGTGCGTGCT GAGCTACGTG GCGGGCATCG CCTGGTTCTT GGCGCTGGTT 
TTCCCGCCGC TGACCCAGCG CACTTACATG TCGGAGAACG CCATGGGCTC CACCATGGTG 
GAGGAGCAGT TTGCGGGCGG AGACCGTGCC CGGGCTTTTG CCCGGGACTT CGCCGCCCAC 
CGCAAGAAGT CGGGGGCTCT GCCAGTGGCC TGGCTTGAAC GGACGATGCG GTCAGTAGGG 
CTGGAGGTCT ACACGCAGAG TTTCTCCCGG AAACTGCCCT TCCCAGATGA GACCCACGAG 
CGCTATATGG TGTCGGGCAC CAACGTGTAC GGCATCCTGC GGGCCCCGCG TGCTGCCAGC 
ACCGAGTCGC TTGTGCTCAC CGTGCCCTGT GGCTCTGACT CTACCAACAG CCAGGCTGTG 
GGGCTGCTGC TGGCACTGGC TGCCCACTTC CGGGGGCAGA TTTATTGGGC CAAAGATATC 
GTCTTCCTGG TAACAGAACA TGACCTTCTG GGCACTGAGG CTTGGCTTGA AGCCTACCAC 
GATGTCAATG TCACTGGCAT GCAGTCGTCT CCCCTGCAGG GCCGAGCTGG GGCCATTCAG 
GCAGCCGTGG CCCTGGAGCT GAGCAGTGAT GTGGTCACCA GCCTCGATGT GGCCGTGGAG 
GGGCTTAACG GGCAGCTGCC CAACCTTGAC CTGCTCAATC TCTTCCAGAC CTTCTGCCAG 
AAAGGGGGCC TGTTGTGCAC GCTTCAGGGC AAGCTGCAGC CCGAGGACTG GACATCATTG 
GATGGACCGC TGCAGGGCCT GCAGACACTG CTGCTCATGG TTCTGCGGCA GGCCTCCGGC 
CGCCCCCACG GCTCCCATGG CCTCTTCCTG CGCTACCGTG TGGAGGCCCT AACCCTGCGT 
GGCATCAATA GCTTCCGCCA GTACAAGTAT GACCTGGTGG CAGTGGGCAA GGCTTTGGAG 
GGCATGTTCC GCAAGCTCAA CCACCTCCTG GAGCGCCTGC ACCAGTCCTT CTTCCTCTAC 
TTGCTCCCCG GCCTCTCCCG CTTCGTCTCC ATCGGCCTCT ACATGCCCGC TGTCGGCTTC 
TTGCTCCTGG TCCTTGGTCT CAAGGCTCTG GAACTGTGGA TGCAGCTGCA TGAGGCTGGA 
ATGGGCCTTG AGGAGCCCGG GGGTGCCCCT GGCCCCAGTG TACCCCTTCC CCCATCACAG 
GGTGTGGGGC TGGCCTCGCT CGTGGCACCT CTGCTGATCT CACAGGCCAT GGGACTGGCC 
CTCTATGTCC TGCCAGTGCT GGGCCAACAC GTTGCCACCC AGCACTTCCC AGTGGCAGAG 
GCTGAGGCTG TGGTGCTGAC ACTGCTGGCG ATTTATGCAG CTGGCCTGGC CCTGCCCCAC 
AATACCCACC GGGTGGTAAG CACACAGGCC CCAGACAGGG GCTGGATGGC ACTGAAGCTG 
GTAGCCCTGA TCTACCTAGC ACTGCAGCTG GGCTGCATCG CCCTCACCAA CTTCTCACTG 
GGCTTCCTGC TGGCCACCAC CATGGTGCCC ACTGCTGCGC TTGCCAAGCC TCATGGGCCC 
CGGACCCTCT ATGCTGCCCT GCTGGTGCTG ACCAGCCCGG CAGCCACGCT CCTTGGCAGC 
CTGTTCCTGT GGCGGGAGCT GCAGGAGGCG CCACTGTCAC TGGCCGAGGG CTGGCAGCTC 
TTCCTGGCAG CGCTAGCCCA GGGTGTGCTG GAGCACCACA CCTACGGCGC CCTGCTCTTC 
CCACTGCTGT CCCTGGGCCT CTACCCCTGC TGGCTGCTTT TCTGGAATGT GCTCTTCTGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003792.1
CDS	98..1963
Translation	MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMVEEQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLFWK

Target ORF information:

RefSeq Version	NM_003801.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycosylphosphatidylinositol anchor attachment 1 (GPAA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003801.4

ORF Insert Sequence:

ATGGGCCTCC TGTCGGACCC GGTTCGCCGG CGCGCGCTCG CCCGCCTAGT GCTGCGCCTC 
AACGCGCCGT TGTGCGTGCT GAGCTACGTG GCGGGCATCG CCTGGTTCTT GGCGCTGGTT 
TTCCCGCCGC TGACCCAGCG CACTTACATG TCGGAGAACG CCATGGGCTC CACCATGGTG 
GAGGAGCAGT TTGCGGGCGG AGACCGTGCC CGGGCTTTTG CCCGGGACTT CGCCGCCCAC 
CGCAAGAAGT CGGGGGCTCT GCCAGTGGCC TGGCTTGAAC GGACGATGCG GTCAGTAGGG 
CTGGAGGTCT ACACGCAGAG TTTCTCCCGG AAACTGCCCT TCCCAGATGA GACCCACGAG 
CGCTATATGG TGTCGGGCAC CAACGTGTAC GGCATCCTGC GGGCCCCGCG TGCTGCCAGC 
ACCGAGTCGC TTGTGCTCAC CGTGCCCTGT GGCTCTGACT CTACCAACAG CCAGGCTGTG 
GGGCTGCTGC TGGCACTGGC TGCCCACTTC CGGGGGCAGA TTTATTGGGC CAAAGATATC 
GTCTTCCTGG TAACAGAACA TGACCTTCTG GGCACTGAGG CTTGGCTTGA AGCCTACCAC 
GATGTCAATG TCACTGGCAT GCAGTCGTCT CCCCTGCAGG GCCGAGCTGG GGCCATTCAG 
GCAGCCGTGG CCCTGGAGCT GAGCAGTGAT GTGGTCACCA GCCTCGATGT GGCCGTGGAG 
GGGCTTAACG GGCAGCTGCC CAACCTTGAC CTGCTCAATC TCTTCCAGAC CTTCTGCCAG 
AAAGGGGGCC TGTTGTGCAC GCTTCAGGGC AAGCTGCAGC CCGAGGACTG GACATCATTG 
GATGGACCGC TGCAGGGCCT GCAGACACTG CTGCTCATGG TTCTGCGGCA GGCCTCCGGC 
CGCCCCCACG GCTCCCATGG CCTCTTCCTG CGCTACCGTG TGGAGGCCCT AACCCTGCGT 
GGCATCAATA GCTTCCGCCA GTACAAGTAT GACCTGGTGG CAGTGGGCAA GGCTTTGGAG 
GGCATGTTCC GCAAGCTCAA CCACCTCCTG GAGCGCCTGC ACCAGTCCTT CTTCCTCTAC 
TTGCTCCCCG GCCTCTCCCG CTTCGTCTCC ATCGGCCTCT ACATGCCCGC TGTCGGCTTC 
TTGCTCCTGG TCCTTGGTCT CAAGGCTCTG GAACTGTGGA TGCAGCTGCA TGAGGCTGGA 
ATGGGCCTTG AGGAGCCCGG GGGTGCCCCT GGCCCCAGTG TACCCCTTCC CCCATCACAG 
GGTGTGGGGC TGGCCTCGCT CGTGGCACCT CTGCTGATCT CACAGGCCAT GGGACTGGCC 
CTCTATGTCC TGCCAGTGCT GGGCCAACAC GTTGCCACCC AGCACTTCCC AGTGGCAGAG 
GCTGAGGCTG TGGTGCTGAC ACTGCTGGCG ATTTATGCAG CTGGCCTGGC CCTGCCCCAC 
AATACCCACC GGGTGGTAAG CACACAGGCC CCAGACAGGG GCTGGATGGC ACTGAAGCTG 
GTAGCCCTGA TCTACCTAGC ACTGCAGCTG GGCTGCATCG CCCTCACCAA CTTCTCACTG 
GGCTTCCTGC TGGCCACCAC CATGGTGCCC ACTGCTGCGC TTGCCAAGCC TCATGGGCCC 
CGGACCCTCT ATGCTGCCCT GCTGGTGCTG ACCAGCCCGG CAGCCACGCT CCTTGGCAGC 
CTGTTCCTGT GGCGGGAGCT GCAGGAGGCG CCACTGTCAC TGGCCGAGGG CTGGCAGCTC 
TTCCTGGCAG CGCTAGCCCA GGGTGTGCTG GAGCACCACA CCTACGGCGC CCTGCTCTTC 
CCACTGCTGT CCCTGGGCCT CTACCCCTGC TGGCTGCTTT TCTGGAATGT GCTCTTCTGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in GPAA1, Encoding a GPI Transamidase Complex Protein, Cause Developmental Delay, Epilepsy, Cerebellar Atrophy, and Osteopenia.
American journal of human genetics101(5)856-865(2017 Nov)
Nguyen TTM,Murakami Y,Sheridan E,Ehresmann S,Rousseau J,St-Denis A,Chai G,Ajeawung NF,Fairbrother L,Reimschisel T,Bateman A,Berry-Kravis E,Xia F,Tardif J,Parry DA,Logan CV,Diggle C,Bennett CP,Hattingh L,Rosenfeld JA,Perry MS,Parker MJ,Le Deist F,Zaki MS,Ignatius E,Isohanni P,L?nnqvist T,Carroll CJ,Johnson CA,Gleeson JG,Kinoshita T,Campeau PM

Transamidase subunit GAA1/GPAA1 is a M28 family metallo-peptide-synthetase that catalyzes the peptide bond formation between the substrate protein's omega-site and the GPI lipid anchor's phosphoethanolamine.
Cell cycle (Georgetown, Tex.)13(12)1912-7(2014)
Eisenhaber B,Eisenhaber S,Kwang TY,Gr?ber G,Eisenhaber F

Human metabolic individuality in biomedical and pharmaceutical research.
Nature477(7362)54-60(2011 Aug)
Suhre K,Shin SY,Petersen AK,Mohney RP,Meredith D,W?gele B,Altmaier E,,Deloukas P,Erdmann J,Grundberg E,Hammond CJ,de Angelis MH,Kastenm?ller G,K?ttgen A,Kronenberg F,Mangino M,Meisinger C,Meitinger T,Mewes HW,Milburn MV,Prehn C,Raffler J,Ried JS,R?misch-Margl W,Samani NJ,Small KS,Wichmann HE,Zhai G,Illig T,Spector TD,Adamski J,Soranzo N,Gieger C

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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GPAA1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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