CBR1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CBR1
Entrez Gene ID	873
Full Name	carbonyl reductase 1
Synonyms	CBR,SDR21C1,hCBR1
General protein information	Preferred Names carbonyl reductase 1 Names carbonyl reductase [NADPH] 1 15-hydroxyprostaglandin dehydrogenase NADPH-dependent carbonyl reductase 1 carbonyl reductase (NADPH) 1 prostaglandin 9-ketoreductase prostaglandin-E(2) 9-reductase short chain dehydrogenase/reductase family 21C member 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.12
Summary	The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013].
Disorder MIM:	114830

mRNA and Protein(s)

mRNA	Protein	Name
NM_001757.3	NP_001748.1	carbonyl reductase [NADPH] 1 isoform 1
NM_001286789.2	NP_001273718.1	carbonyl reductase [NADPH] 1 isoform 2
NM_001757.4	NP_001748.1	carbonyl reductase [NADPH] 1 isoform 1
NM_001286789.1	NP_001273718.1	carbonyl reductase [NADPH] 1 isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cbr1	NP_062043.1
Gallus gallus (chicken)	CBR1	NP_001025966.1
	CBR1	XP_001088120.2
Bos taurus (cattle)	CBR1	NP_001029685.1
Pan troglodytes (chimpanzee)	CBR1	XP_531449.2
Canis lupus familiaris (dog)	LOC610164	XP_852675.1
Rattus norvegicus (Norway rat)	LOC102556347	XP_006248171.1
Rattus norvegicus (Norway rat)	LOC100360507	XP_003751064.2
Oryza sativa (rice)	Os01g0929500	NP_001045283.1
Xenopus tropicalis (tropical clawed frog)	cbr1	NP_001011190.1
Homo sapiens (human)	CBR1	NP_001748.1
Arabidopsis thaliana (thale cress)	AT5G61830	NP_200991.1
Xenopus tropicalis (tropical clawed frog)	cbr3	NP_001120040.1
Xenopus tropicalis (tropical clawed frog)	LOC101735101	XP_004912181.1
Mus musculus (house mouse)	Cbr1	NP_031646.2
Danio rerio (zebrafish)	cbr1	NP_919387.1
Arabidopsis thaliana (thale cress)	AT5G51030	NP_199916.1

Gene Ontology
Bibliography

Related articles in PubMed

Kinetic features of carbonyl reductase 1 acting on glutathionylated aldehydes.
Moschini R, Rotondo R, Renzone G, Balestri F, Cappiello M, Scaloni A, Mura U, Del-Corso A
Chemico-biological interactions276127-132(2017 Oct)

Carbonyl reductase 1 catalyzes 20?-reduction of glucocorticoids, modulating receptor activation and metabolic complications of obesity.
Morgan RA, Beck KR, Nixon M, Homer NZM, Crawford AA, Melchers D, Houtman R, Meijer OC, Stomby A, Anderson AJ, Upreti R, Stimson RH, Olsson T, Michoel T, Cohain A, Ruusalepp A, Schadt EE, Bj?rkegren JLM, Andrew R, Kenyon CJ, Hadoke PWF, Odermatt A, Keen JA, Walker BR
Scientific reports7(1)10633(2017 Sep)

Human carbonyl reductase 1 participating in intestinal first-pass drug metabolism is inhibited by fatty acids and acyl-CoAs.
Hara A, Endo S, Matsunaga T, El-Kabbani O, Miura T, Nishinaka T, Terada T
Biochemical pharmacology138185-192(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The ability of human carbonyl reductase 1 to efficiently catalyze the reduction of glutathionylated aldehydes derived from lipid peroxidation, that in the case of glutathionylated-4-hydroxyalkanals is associated to the ability to oxidize the hemiacetal hydroxyl group
Title: Kinetic features of carbonyl reductase 1 acting on glutathionylated aldehydes.

Data suggest that fatty acids and acyl-CoAs bind competitively with respect to substrate binding to carbonyl reductase 1 (CBR1), an enzyme involved in first-pass drug metabolism in intestinal mucosa; inhibition of CBR1 by these products of digestion may lead to food-drug interactions.
Title: Human carbonyl reductase 1 participating in intestinal first-pass drug metabolism is inhibited by fatty acids and acyl-CoAs.

CRB1 is an efficient catalyst for the reduction of glutathionylated aldehydes derived from lipid peroxidation.
Title: Human carbonyl reductase 1 as efficient catalyst for the reduction of glutathionylated aldehydes derived from lipid peroxidation.

AKR1C3 is the primary enzyme and CBR1 is a minor enzyme responsible for warfarin reduction in human liver cytosol.
Title: Carbonyl reduction of warfarin: Identification and characterization of human warfarin reductases.

These results suggest that CR1 induces apoptosis by activating the caspase pathway via binding to TNFR1.
Title: Inhibitory effect of carbonyl reductase 1 on ovarian cancer growth via tumor necrosis factor receptor signaling.

The following CBR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CBR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00915	NM_001757.4 Latest version!	Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu05719	NM_001286789.2 Latest version!	Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu00915	NM_001757.3	Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu05719	NM_001286789.1	Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CBR1 ( NM_001757.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001748.1 Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00915
Clone ID Related Accession (Same CDS sequence)	NM_001757.3 , NM_001757.4
Accession Version	NM_001757.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	carbonyl reductase [NADPH] 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB124848.1 and N51716.1. On Nov 15, 2013 this sequence version replaced NM_001757.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB124848.1, SRR1163658.73241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCCG GCATCCATGT AGCGCTGGTG ACTGGAGGCA ACAAGGGCAT CGGCTTGGCC 
ATCGTGCGCG ACCTGTGCCG GCTGTTCTCG GGGGACGTGG TGCTCACGGC GCGGGACGTG 
ACGCGGGGCC AGGCGGCCGT ACAGCAGCTG CAGGCGGAGG GCCTGAGCCC GCGCTTCCAC 
CAGCTGGACA TCGACGATCT GCAGAGCATC CGCGCCCTGC GCGACTTCCT GCGCAAGGAG 
TACGGGGGCC TGGACGTGCT GGTCAACAAC GCGGGCATCG CCTTCAAGGT TGCTGATCCC 
ACACCCTTTC ATATTCAAGC TGAAGTGACG ATGAAAACAA ATTTCTTTGG TACCCGAGAT 
GTGTGCACAG AATTACTCCC TCTAATAAAA CCCCAAGGGA GAGTGGTGAA CGTATCTAGC 
ATCATGAGCG TCAGAGCCCT TAAAAGCTGC AGCCCAGAGC TGCAGCAGAA GTTCCGCAGT 
GAGACCATCA CTGAGGAGGA GCTGGTGGGG CTCATGAACA AGTTTGTGGA GGATACAAAG 
AAGGGAGTGC ACCAGAAGGA GGGCTGGCCC AGCAGCGCAT ACGGGGTGAC GAAGATTGGC 
GTCACCGTTC TGTCCAGGAT CCACGCCAGG AAACTGAGTG AGCAGAGGAA AGGGGACAAG 
ATCCTCCTGA ATGCCTGCTG CCCAGGGTGG GTGAGAACTG ACATGGCGGG ACCCAAGGCC 
ACCAAGAGCC CAGAAGAAGG TGCAGAGACC CCTGTGTACT TGGCCCTTTT GCCCCCAGAT 
GCTGAGGGTC CCCATGGACA ATTTGTTTCA GAGAAGAGAG TTGAACAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001748.1
CDS	193..1026
Translation	MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQW

Target ORF information:

RefSeq Version	NM_001757.3
Organism	Homo sapiens(human)
Definition	Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001757.3

ORF Insert Sequence:

ATGTCGTCCG GCATCCATGT AGCGCTGGTG ACTGGAGGCA ACAAGGGCAT CGGCTTGGCC 
ATCGTGCGCG ACCTGTGCCG GCTGTTCTCG GGGGACGTGG TGCTCACGGC GCGGGACGTG 
ACGCGGGGCC AGGCGGCCGT ACAGCAGCTG CAGGCGGAGG GCCTGAGCCC GCGCTTCCAC 
CAGCTGGACA TCGACGATCT GCAGAGCATC CGCGCCCTGC GCGACTTCCT GCGCAAGGAG 
TACGGGGGCC TGGACGTGCT GGTCAACAAC GCGGGCATCG CCTTCAAGGT TGCTGATCCC 
ACACCCTTTC ATATTCAAGC TGAAGTGACG ATGAAAACAA ATTTCTTTGG TACCCGAGAT 
GTGTGCACAG AATTACTCCC TCTAATAAAA CCCCAAGGGA GAGTGGTGAA CGTATCTAGC 
ATCATGAGCG TCAGAGCCCT TAAAAGCTGC AGCCCAGAGC TGCAGCAGAA GTTCCGCAGT 
GAGACCATCA CTGAGGAGGA GCTGGTGGGG CTCATGAACA AGTTTGTGGA GGATACAAAG 
AAGGGAGTGC ACCAGAAGGA GGGCTGGCCC AGCAGCGCAT ACGGGGTGAC GAAGATTGGC 
GTCACCGTTC TGTCCAGGAT CCACGCCAGG AAACTGAGTG AGCAGAGGAA AGGGGACAAG 
ATCCTCCTGA ATGCCTGCTG CCCAGGGTGG GTGAGAACTG ACATGGCGGG ACCCAAGGCC 
ACCAAGAGCC CAGAAGAAGG TGCAGAGACC CCTGTGTACT TGGCCCTTTT GCCCCCAGAT 
GCTGAGGGTC CCCATGGACA ATTTGTTTCA GAGAAGAGAG TTGAACAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CBR1 ( NM_001757.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001748.1 Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00915
Clone ID Related Accession (Same CDS sequence)	NM_001757.3 , NM_001757.4
Accession Version	NM_001757.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	carbonyl reductase [NADPH] 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB124848.1. On Nov 22, 2018 this sequence version replaced NM_001757.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB124848.1, J04056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290349.11/ ENSP00000290349.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGTCCG GCATCCATGT AGCGCTGGTG ACTGGAGGCA ACAAGGGCAT CGGCTTGGCC 
ATCGTGCGCG ACCTGTGCCG GCTGTTCTCG GGGGACGTGG TGCTCACGGC GCGGGACGTG 
ACGCGGGGCC AGGCGGCCGT ACAGCAGCTG CAGGCGGAGG GCCTGAGCCC GCGCTTCCAC 
CAGCTGGACA TCGACGATCT GCAGAGCATC CGCGCCCTGC GCGACTTCCT GCGCAAGGAG 
TACGGGGGCC TGGACGTGCT GGTCAACAAC GCGGGCATCG CCTTCAAGGT TGCTGATCCC 
ACACCCTTTC ATATTCAAGC TGAAGTGACG ATGAAAACAA ATTTCTTTGG TACCCGAGAT 
GTGTGCACAG AATTACTCCC TCTAATAAAA CCCCAAGGGA GAGTGGTGAA CGTATCTAGC 
ATCATGAGCG TCAGAGCCCT TAAAAGCTGC AGCCCAGAGC TGCAGCAGAA GTTCCGCAGT 
GAGACCATCA CTGAGGAGGA GCTGGTGGGG CTCATGAACA AGTTTGTGGA GGATACAAAG 
AAGGGAGTGC ACCAGAAGGA GGGCTGGCCC AGCAGCGCAT ACGGGGTGAC GAAGATTGGC 
GTCACCGTTC TGTCCAGGAT CCACGCCAGG AAACTGAGTG AGCAGAGGAA AGGGGACAAG 
ATCCTCCTGA ATGCCTGCTG CCCAGGGTGG GTGAGAACTG ACATGGCGGG ACCCAAGGCC 
ACCAAGAGCC CAGAAGAAGG TGCAGAGACC CCTGTGTACT TGGCCCTTTT GCCCCCAGAT 
GCTGAGGGTC CCCATGGACA ATTTGTTTCA GAGAAGAGAG TTGAACAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001748.1
CDS	93..926
Translation	MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQW

Target ORF information:

RefSeq Version	NM_001757.4
Organism	Homo sapiens(human)
Definition	Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001757.4

ORF Insert Sequence:

ATGTCGTCCG GCATCCATGT AGCGCTGGTG ACTGGAGGCA ACAAGGGCAT CGGCTTGGCC 
ATCGTGCGCG ACCTGTGCCG GCTGTTCTCG GGGGACGTGG TGCTCACGGC GCGGGACGTG 
ACGCGGGGCC AGGCGGCCGT ACAGCAGCTG CAGGCGGAGG GCCTGAGCCC GCGCTTCCAC 
CAGCTGGACA TCGACGATCT GCAGAGCATC CGCGCCCTGC GCGACTTCCT GCGCAAGGAG 
TACGGGGGCC TGGACGTGCT GGTCAACAAC GCGGGCATCG CCTTCAAGGT TGCTGATCCC 
ACACCCTTTC ATATTCAAGC TGAAGTGACG ATGAAAACAA ATTTCTTTGG TACCCGAGAT 
GTGTGCACAG AATTACTCCC TCTAATAAAA CCCCAAGGGA GAGTGGTGAA CGTATCTAGC 
ATCATGAGCG TCAGAGCCCT TAAAAGCTGC AGCCCAGAGC TGCAGCAGAA GTTCCGCAGT 
GAGACCATCA CTGAGGAGGA GCTGGTGGGG CTCATGAACA AGTTTGTGGA GGATACAAAG 
AAGGGAGTGC ACCAGAAGGA GGGCTGGCCC AGCAGCGCAT ACGGGGTGAC GAAGATTGGC 
GTCACCGTTC TGTCCAGGAT CCACGCCAGG AAACTGAGTG AGCAGAGGAA AGGGGACAAG 
ATCCTCCTGA ATGCCTGCTG CCCAGGGTGG GTGAGAACTG ACATGGCGGG ACCCAAGGCC 
ACCAAGAGCC CAGAAGAAGG TGCAGAGACC CCTGTGTACT TGGCCCTTTT GCCCCCAGAT 
GCTGAGGGTC CCCATGGACA ATTTGTTTCA GAGAAGAGAG TTGAACAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CBR1 ( NM_001286789.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273718.1 Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05719
Clone ID Related Accession (Same CDS sequence)	NM_001286789.2 , NM_001286789.1
Accession Version	NM_001286789.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	carbonyl reductase [NADPH] 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK294142.1 and AK311219.1. On May 31, 2019 this sequence version replaced NM_001286789.1. Transcript Variant: This variant (2) uses an alternate acceptor splice site at the 3' terminal exon that causes a frame-shift compared to variant 1. The resulting shorter isoform (2) has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.235468.1, SRR1803614.180422.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCCG GCATCCATGT AGCGCTGGTG ACTGGAGGCA ACAAGGGCAT CGGCTTGGCC 
ATCGTGCGCG ACCTGTGCCG GCTGTTCTCG GGGGACGTGG TGCTCACGGC GCGGGACGTG 
ACGCGGGGCC AGGCGGCCGT ACAGCAGCTG CAGGCGGAGG GCCTGAGCCC GCGCTTCCAC 
CAGCTGGACA TCGACGATCT GCAGAGCATC CGCGCCCTGC GCGACTTCCT GCGCAAGGAG 
TACGGGGGCC TGGACGTGCT GGTCAACAAC GCGGGCATCG CCTTCAAGGT TGCTGATCCC 
ACACCCTTTC ATATTCAAGC TGAAGTGACG ATGAAAACAA ATTTCTTTGG TACCCGAGAT 
GTGTGCACAG AATTACTCCC TCTAATAAAA CCCCAAGCAT CCTGCGTACT GTCTGCATGG 
TCATGCCTCT CCCAAAATCC TTCAGGAGGA AAGTCCAAGC CCTTAGCATG GTTCACAGAG 
ATGTCCATAA TCTGCCGCTG CTTAACTCTG GGCCCATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273718.1
CDS	93..614
Translation	MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQASCVLSAWSCLSQNPSGGKSKPLAWFTEMSIICRCLTLGPF

Target ORF information:

RefSeq Version	NM_001286789.2
Organism	Homo sapiens(human)
Definition	Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286789.2

ORF Insert Sequence:

ATGTCGTCCG GCATCCATGT AGCGCTGGTG ACTGGAGGCA ACAAGGGCAT CGGCTTGGCC 
ATCGTGCGCG ACCTGTGCCG GCTGTTCTCG GGGGACGTGG TGCTCACGGC GCGGGACGTG 
ACGCGGGGCC AGGCGGCCGT ACAGCAGCTG CAGGCGGAGG GCCTGAGCCC GCGCTTCCAC 
CAGCTGGACA TCGACGATCT GCAGAGCATC CGCGCCCTGC GCGACTTCCT GCGCAAGGAG 
TACGGGGGCC TGGACGTGCT GGTCAACAAC GCGGGCATCG CCTTCAAGGT TGCTGATCCC 
ACACCCTTTC ATATTCAAGC TGAAGTGACG ATGAAAACAA ATTTCTTTGG TACCCGAGAT 
GTGTGCACAG AATTACTCCC TCTAATAAAA CCCCAAGCAT CCTGCGTACT GTCTGCATGG 
TCATGCCTCT CCCAAAATCC TTCAGGAGGA AAGTCCAAGC CCTTAGCATG GTTCACAGAG 
ATGTCCATAA TCTGCCGCTG CTTAACTCTG GGCCCATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CBR1 ( NM_001286789.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273718.1 Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05719
Clone ID Related Accession (Same CDS sequence)	NM_001286789.2 , NM_001286789.1
Accession Version	NM_001286789.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	carbonyl reductase [NADPH] 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB124848.1, AK294142.1, AK311219.1 and N51716.1. On Nov 15, 2013 this sequence version replaced XM_005261073.1. Transcript Variant: This variant (2) uses an alternate acceptor splice site at the 3' terminal exon that causes a frame-shift compared to variant 1. The resulting shorter isoform (2) has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK311219.1, SRR1803613.276432.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCCG GCATCCATGT AGCGCTGGTG ACTGGAGGCA ACAAGGGCAT CGGCTTGGCC 
ATCGTGCGCG ACCTGTGCCG GCTGTTCTCG GGGGACGTGG TGCTCACGGC GCGGGACGTG 
ACGCGGGGCC AGGCGGCCGT ACAGCAGCTG CAGGCGGAGG GCCTGAGCCC GCGCTTCCAC 
CAGCTGGACA TCGACGATCT GCAGAGCATC CGCGCCCTGC GCGACTTCCT GCGCAAGGAG 
TACGGGGGCC TGGACGTGCT GGTCAACAAC GCGGGCATCG CCTTCAAGGT TGCTGATCCC 
ACACCCTTTC ATATTCAAGC TGAAGTGACG ATGAAAACAA ATTTCTTTGG TACCCGAGAT 
GTGTGCACAG AATTACTCCC TCTAATAAAA CCCCAAGCAT CCTGCGTACT GTCTGCATGG 
TCATGCCTCT CCCAAAATCC TTCAGGAGGA AAGTCCAAGC CCTTAGCATG GTTCACAGAG 
ATGTCCATAA TCTGCCGCTG CTTAACTCTG GGCCCATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273718.1
CDS	193..714
Translation	MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQASCVLSAWSCLSQNPSGGKSKPLAWFTEMSIICRCLTLGPF

Target ORF information:

RefSeq Version	NM_001286789.1
Organism	Homo sapiens(human)
Definition	Homo sapiens carbonyl reductase 1 (CBR1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286789.1

ORF Insert Sequence:

ATGTCGTCCG GCATCCATGT AGCGCTGGTG ACTGGAGGCA ACAAGGGCAT CGGCTTGGCC 
ATCGTGCGCG ACCTGTGCCG GCTGTTCTCG GGGGACGTGG TGCTCACGGC GCGGGACGTG 
ACGCGGGGCC AGGCGGCCGT ACAGCAGCTG CAGGCGGAGG GCCTGAGCCC GCGCTTCCAC 
CAGCTGGACA TCGACGATCT GCAGAGCATC CGCGCCCTGC GCGACTTCCT GCGCAAGGAG 
TACGGGGGCC TGGACGTGCT GGTCAACAAC GCGGGCATCG CCTTCAAGGT TGCTGATCCC 
ACACCCTTTC ATATTCAAGC TGAAGTGACG ATGAAAACAA ATTTCTTTGG TACCCGAGAT 
GTGTGCACAG AATTACTCCC TCTAATAAAA CCCCAAGCAT CCTGCGTACT GTCTGCATGG 
TCATGCCTCT CCCAAAATCC TTCAGGAGGA AAGTCCAAGC CCTTAGCATG GTTCACAGAG 
ATGTCCATAA TCTGCCGCTG CTTAACTCTG GGCCCATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kinetic features of carbonyl reductase 1 acting on glutathionylated aldehydes.
Chemico-biological interactions276127-132(2017 Oct)
Moschini R,Rotondo R,Renzone G,Balestri F,Cappiello M,Scaloni A,Mura U,Del-Corso A

Carbonyl reductase 1 catalyzes 20?-reduction of glucocorticoids, modulating receptor activation and metabolic complications of obesity.
Scientific reports7(1)10633(2017 Sep)
Morgan RA,Beck KR,Nixon M,Homer NZM,Crawford AA,Melchers D,Houtman R,Meijer OC,Stomby A,Anderson AJ,Upreti R,Stimson RH,Olsson T,Michoel T,Cohain A,Ruusalepp A,Schadt EE,Bj?rkegren JLM,Andrew R,Kenyon CJ,Hadoke PWF,Odermatt A,Keen JA,Walker BR

Human carbonyl reductase 1 participating in intestinal first-pass drug metabolism is inhibited by fatty acids and acyl-CoAs.
Biochemical pharmacology138185-192(2017 Aug)
Hara A,Endo S,Matsunaga T,El-Kabbani O,Miura T,Nishinaka T,Terada T

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CBR1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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