B3GALT4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	B3GALT4
Entrez Gene ID	8705
Full Name	beta-1,3-galactosyltransferase 4
Synonyms	BETA3GALT4,GALT2,GALT4
General protein information	Preferred Names beta-1,3-galactosyltransferase 4 Names beta-1,3-galactosyltransferase 4 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase 4 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4 UDP-galactose:beta-N-acetyl-galactosamine-beta-1,3-galactosyltransferase b3Gal-T4 beta-1,3-GalTase 4 beta-3-galactosyltransferase 4 gal-T2 ganglioside galactosyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.32
Summary	This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq, Jul 2008]. COMPLETENESS: complete on the 3' end.
Disorder MIM:	603095

mRNA and Protein(s)

mRNA	Protein	Name
NM_003782.3	NP_003773.1	beta-1,3-galactosyltransferase 4
NM_003782.4	NP_003773.1	beta-1,3-galactosyltransferase 4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	B3GALT4	NP_001074191.1
Pan troglodytes (chimpanzee)	B3GALT4	XP_001170317.1
Bos taurus (cattle)	B3GALT4	NP_001070565.1
Mus musculus (house mouse)	B3galt4	NP_062293.1
Rattus norvegicus (Norway rat)	B3galt4	NP_598237.2
Danio rerio (zebrafish)	LOC101886595	XP_005167416.1
Homo sapiens (human)	B3GALT4	NP_003773.1
	B3GALT4	XP_001116066.1
Xenopus tropicalis (tropical clawed frog)	b3galt4	XP_002938731.1

Gene Ontology
Bibliography

Related articles in PubMed

Induction of GM1a/GD1b synthase triggers complex ganglioside expression and alters neuroblastoma cell behavior; a new tumor cell model of ganglioside function.
Dong L, Liu Y, Colberg-Poley AM, Kaucic K, Ladisch S
Glycoconjugate journal28(3-4)137-47(2011 May)

GM1?/?GD1b?/?GA1 synthase expression results in the reduced cancer phenotypes with modulation of composition and raft-localization of gangliosides in a melanoma cell line.
Dong Y, Ikeda K, Hamamura K, Zhang Q, Kondo Y, Matsumoto Y, Ohmi Y, Yamauchi Y, Furukawa K, Taguchi R, Furukawa K
Cancer science101(9)2039-47(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Induction of GM1a/GD1b synthase triggers complex ganglioside expression and alters neuroblastoma cell behavior; a new tumor cell model of ganglioside function
Title: Induction of GM1a/GD1b synthase triggers complex ganglioside expression and alters neuroblastoma cell behavior; a new tumor cell model of ganglioside function.

Data showed that GM1/GD1b/GA1 synthase transfectant cells had definite reduction in cell growth and invasion.
Title: GM1?/?GD1b?/?GA1 synthase expression results in the reduced cancer phenotypes with modulation of composition and raft-localization of gangliosides in a melanoma cell line.

Observational study of gene-disease association. (HuGE Navigator)
Title: High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions.

We found beta3Gal-T4 and T5 enzymatic activity in ovarian cancer tissues, indicating that these enzymes are expressed at least in ovarian cancer
Title: Beta1,3-galactosyltransferases-4/5 are novel tumor markers for gynecological cancers.

The following B3GALT4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B3GALT4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29876	NM_003782.4 Latest version!	Homo sapiens beta-1,3-galactosyltransferase 4 (B3GALT4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu29876	NM_003782.3	Homo sapiens beta-1,3-galactosyltransferase 4 (B3GALT4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B3GALT4 ( NM_003782.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003773.1 Homo sapiens beta-1,3-galactosyltransferase 4 (B3GALT4), mRNA.

Price & Availability↑

CloneID	OHu29876
Clone ID Related Accession (Same CDS sequence)	NM_003782.3 , NM_003782.4
Accession Version	NM_003782.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	beta-1,3-galactosyltransferase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK075312.1, AB026730.1 and BU740293.1. On Aug 31, 2004 this sequence version replaced NM_003782.2. COMPLETENESS: complete on the 3' end.

ATGCAGCTCA GGCTCTTCCG GCGCCTCCTT CTCGCCGCTT TGCTGCTGGT GATCGTCTGG 
ACCCTCTTCG GGCCTTCGGG GTTGGGGGAG GAGCTGCTGA GCCTCTCACT AGCCTCCCTG 
CTCCCAGCCC CCGCCTCACC GGGGCCGCCC CTGGCCCTGC CCCGCCTCTT GATCCCCAAC 
CAGGAAGCTT GCAGTGGTCC CGGGGCCCCT CCCTTCCTGC TCATCCTGGT GTGCACGGCT 
CCGGAGAACC TGAACCAGAG AAACGCCATT CGGGCTTCGT GGGGCGGGCT GCGCGAGGCC 
CGGGGGCTCA GGGTACAGAC GCTATTCTTG CTGGGAGAGC CGAACGCACA GCACCCCGTG 
TGGGGTTCCC AGGGGAGTGA CCTGGCCTCG GAGTCAGCAG CCCAGGGGGA TATCTTGCAG 
GCCGCCTTCC AGGACTCCTA CCGCAACCTC ACCCTAAAGA CCCTCAGCGG GCTGAACTGG 
GCTGAGAAAC ACTGCCCCAT GGCCCGATAC GTCCTCAAGA CGGACGATGA TGTGTATGTC 
AACGTCCCTG AACTGGTATC AGAGCTGGTC TTGCGAGGGG GCCGTTGGGG GCAATGGGAG 
AGAAGCACGG AACCCCAGAG AGAGGCTGAG CAGGAAGGAG GCCAGGTTTT GCACAGCGAG 
GAAGTGCCTC TTCTGTACTT GGGCCGGGTG CACTGGCGCG TGAACCCCTC TCGGACACCG 
GGGGGCAGGC ACCGCGTATC AGAGGAGCAG TGGCCTCACA CCTGGGGCCC CTTTCCACCC 
TATGCCTCAG GCACGGGGTA TGTGCTGTCA GCGTCTGCTG TGCAGCTCAT TCTCAAGGTG 
GCCAGCCGGG CACCCCTTCT CCCATTAGAG GATGTCTTTG TGGGGGTAAG TGCCCGACGA 
GGAGGCCTCG CCCCAACACA GTGTGTCAAG CTGGCTGGTG CCACCCACTA CCCGCTAGAC 
CGGTGCTGCT ATGGGAAATT CCTGCTGACG TCCCACAGGC TGGACCCCTG GAAGATGCAG 
GAAGCCTGGA AGCTGGTGGG TGGCTCTGAC GGGGAAAGGA CTGCGCCCTT TTGCTCCTGG 
TTCCAGGGAG TCCTGGGCAT CCTGCGGTGT CGAGCAATAG CCTGGCTTCA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003773.1
CDS	281..1417
Translation	MQLRLFRRLLLAALLLVIVWTLFGPSGLGEELLSLSLASLLPAPASPGPPLALPRLLIPNQEACSGPGAPPFLLILVCTAPENLNQRNAIRASWGGLREARGLRVQTLFLLGEPNAQHPVWGSQGSDLASESAAQGDILQAAFQDSYRNLTLKTLSGLNWAEKHCPMARYVLKTDDDVYVNVPELVSELVLRGGRWGQWERSTEPQREAEQEGGQVLHSEEVPLLYLGRVHWRVNPSRTPGGRHRVSEEQWPHTWGPFPPYASGTGYVLSASAVQLILKVASRAPLLPLEDVFVGVSARRGGLAPTQCVKLAGATHYPLDRCCYGKFLLTSHRLDPWKMQEAWKLVGGSDGERTAPFCSWFQGVLGILRCRAIAWLQS

Target ORF information:

RefSeq Version	NM_003782.3
Organism	Homo sapiens(human)
Definition	Homo sapiens beta-1,3-galactosyltransferase 4 (B3GALT4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003782.3

ORF Insert Sequence:

ATGCAGCTCA GGCTCTTCCG GCGCCTCCTT CTCGCCGCTT TGCTGCTGGT GATCGTCTGG 
ACCCTCTTCG GGCCTTCGGG GTTGGGGGAG GAGCTGCTGA GCCTCTCACT AGCCTCCCTG 
CTCCCAGCCC CCGCCTCACC GGGGCCGCCC CTGGCCCTGC CCCGCCTCTT GATCCCCAAC 
CAGGAAGCTT GCAGTGGTCC CGGGGCCCCT CCCTTCCTGC TCATCCTGGT GTGCACGGCT 
CCGGAGAACC TGAACCAGAG AAACGCCATT CGGGCTTCGT GGGGCGGGCT GCGCGAGGCC 
CGGGGGCTCA GGGTACAGAC GCTATTCTTG CTGGGAGAGC CGAACGCACA GCACCCCGTG 
TGGGGTTCCC AGGGGAGTGA CCTGGCCTCG GAGTCAGCAG CCCAGGGGGA TATCTTGCAG 
GCCGCCTTCC AGGACTCCTA CCGCAACCTC ACCCTAAAGA CCCTCAGCGG GCTGAACTGG 
GCTGAGAAAC ACTGCCCCAT GGCCCGATAC GTCCTCAAGA CGGACGATGA TGTGTATGTC 
AACGTCCCTG AACTGGTATC AGAGCTGGTC TTGCGAGGGG GCCGTTGGGG GCAATGGGAG 
AGAAGCACGG AACCCCAGAG AGAGGCTGAG CAGGAAGGAG GCCAGGTTTT GCACAGCGAG 
GAAGTGCCTC TTCTGTACTT GGGCCGGGTG CACTGGCGCG TGAACCCCTC TCGGACACCG 
GGGGGCAGGC ACCGCGTATC AGAGGAGCAG TGGCCTCACA CCTGGGGCCC CTTTCCACCC 
TATGCCTCAG GCACGGGGTA TGTGCTGTCA GCGTCTGCTG TGCAGCTCAT TCTCAAGGTG 
GCCAGCCGGG CACCCCTTCT CCCATTAGAG GATGTCTTTG TGGGGGTAAG TGCCCGACGA 
GGAGGCCTCG CCCCAACACA GTGTGTCAAG CTGGCTGGTG CCACCCACTA CCCGCTAGAC 
CGGTGCTGCT ATGGGAAATT CCTGCTGACG TCCCACAGGC TGGACCCCTG GAAGATGCAG 
GAAGCCTGGA AGCTGGTGGG TGGCTCTGAC GGGGAAAGGA CTGCGCCCTT TTGCTCCTGG 
TTCCAGGGAG TCCTGGGCAT CCTGCGGTGT CGAGCAATAG CCTGGCTTCA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B3GALT4 ( NM_003782.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003773.1 Homo sapiens beta-1,3-galactosyltransferase 4 (B3GALT4), mRNA.

Price & Availability↑

CloneID	OHu29876
Clone ID Related Accession (Same CDS sequence)	NM_003782.3 , NM_003782.4
Accession Version	NM_003782.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	beta-1,3-galactosyltransferase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL662820.6, AK075312.1, AB026730.1 and BU740293.1. On May 17, 2019 this sequence version replaced NM_003782.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC032574.1, SRR3476690.1081162.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000451237.3/ ENSP00000390784.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGCTCA GGCTCTTCCG GCGCCTCCTT CTCGCCGCTT TGCTGCTGGT GATCGTCTGG 
ACCCTCTTCG GGCCTTCGGG GTTGGGGGAG GAGCTGCTGA GCCTCTCACT AGCCTCCCTG 
CTCCCAGCCC CCGCCTCACC GGGGCCGCCC CTGGCCCTGC CCCGCCTCTT GATCCCCAAC 
CAGGAAGCTT GCAGTGGTCC CGGGGCCCCT CCCTTCCTGC TCATCCTGGT GTGCACGGCT 
CCGGAGAACC TGAACCAGAG AAACGCCATT CGGGCTTCGT GGGGCGGGCT GCGCGAGGCC 
CGGGGGCTCA GGGTACAGAC GCTATTCTTG CTGGGAGAGC CGAACGCACA GCACCCCGTG 
TGGGGTTCCC AGGGGAGTGA CCTGGCCTCG GAGTCAGCAG CCCAGGGGGA TATCTTGCAG 
GCCGCCTTCC AGGACTCCTA CCGCAACCTC ACCCTAAAGA CCCTCAGCGG GCTGAACTGG 
GCTGAGAAAC ACTGCCCCAT GGCCCGATAC GTCCTCAAGA CGGACGATGA TGTGTATGTC 
AACGTCCCTG AACTGGTATC AGAGCTGGTC TTGCGAGGGG GCCGTTGGGG GCAATGGGAG 
AGAAGCACGG AACCCCAGAG AGAGGCTGAG CAGGAAGGAG GCCAGGTTTT GCACAGCGAG 
GAAGTGCCTC TTCTGTACTT GGGCCGGGTG CACTGGCGCG TGAACCCCTC TCGGACACCG 
GGGGGCAGGC ACCGCGTATC AGAGGAGCAG TGGCCTCACA CCTGGGGCCC CTTTCCACCC 
TATGCCTCAG GCACGGGGTA TGTGCTGTCA GCGTCTGCTG TGCAGCTCAT TCTCAAGGTG 
GCCAGCCGGG CACCCCTTCT CCCATTAGAG GATGTCTTTG TGGGGGTAAG TGCCCGACGA 
GGAGGCCTCG CCCCAACACA GTGTGTCAAG CTGGCTGGTG CCACCCACTA CCCGCTAGAC 
CGGTGCTGCT ATGGGAAATT CCTGCTGACG TCCCACAGGC TGGACCCCTG GAAGATGCAG 
GAAGCCTGGA AGCTGGTGGG TGGCTCTGAC GGGGAAAGGA CTGCGCCCTT TTGCTCCTGG 
TTCCAGGGAG TCCTGGGCAT CCTGCGGTGT CGAGCAATAG CCTGGCTTCA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003773.1
CDS	298..1434
Translation	MQLRLFRRLLLAALLLVIVWTLFGPSGLGEELLSLSLASLLPAPASPGPPLALPRLLIPNQEACSGPGAPPFLLILVCTAPENLNQRNAIRASWGGLREARGLRVQTLFLLGEPNAQHPVWGSQGSDLASESAAQGDILQAAFQDSYRNLTLKTLSGLNWAEKHCPMARYVLKTDDDVYVNVPELVSELVLRGGRWGQWERSTEPQREAEQEGGQVLHSEEVPLLYLGRVHWRVNPSRTPGGRHRVSEEQWPHTWGPFPPYASGTGYVLSASAVQLILKVASRAPLLPLEDVFVGVSARRGGLAPTQCVKLAGATHYPLDRCCYGKFLLTSHRLDPWKMQEAWKLVGGSDGERTAPFCSWFQGVLGILRCRAIAWLQS

Target ORF information:

RefSeq Version	NM_003782.4
Organism	Homo sapiens(human)
Definition	Homo sapiens beta-1,3-galactosyltransferase 4 (B3GALT4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003782.4

ORF Insert Sequence:

ATGCAGCTCA GGCTCTTCCG GCGCCTCCTT CTCGCCGCTT TGCTGCTGGT GATCGTCTGG 
ACCCTCTTCG GGCCTTCGGG GTTGGGGGAG GAGCTGCTGA GCCTCTCACT AGCCTCCCTG 
CTCCCAGCCC CCGCCTCACC GGGGCCGCCC CTGGCCCTGC CCCGCCTCTT GATCCCCAAC 
CAGGAAGCTT GCAGTGGTCC CGGGGCCCCT CCCTTCCTGC TCATCCTGGT GTGCACGGCT 
CCGGAGAACC TGAACCAGAG AAACGCCATT CGGGCTTCGT GGGGCGGGCT GCGCGAGGCC 
CGGGGGCTCA GGGTACAGAC GCTATTCTTG CTGGGAGAGC CGAACGCACA GCACCCCGTG 
TGGGGTTCCC AGGGGAGTGA CCTGGCCTCG GAGTCAGCAG CCCAGGGGGA TATCTTGCAG 
GCCGCCTTCC AGGACTCCTA CCGCAACCTC ACCCTAAAGA CCCTCAGCGG GCTGAACTGG 
GCTGAGAAAC ACTGCCCCAT GGCCCGATAC GTCCTCAAGA CGGACGATGA TGTGTATGTC 
AACGTCCCTG AACTGGTATC AGAGCTGGTC TTGCGAGGGG GCCGTTGGGG GCAATGGGAG 
AGAAGCACGG AACCCCAGAG AGAGGCTGAG CAGGAAGGAG GCCAGGTTTT GCACAGCGAG 
GAAGTGCCTC TTCTGTACTT GGGCCGGGTG CACTGGCGCG TGAACCCCTC TCGGACACCG 
GGGGGCAGGC ACCGCGTATC AGAGGAGCAG TGGCCTCACA CCTGGGGCCC CTTTCCACCC 
TATGCCTCAG GCACGGGGTA TGTGCTGTCA GCGTCTGCTG TGCAGCTCAT TCTCAAGGTG 
GCCAGCCGGG CACCCCTTCT CCCATTAGAG GATGTCTTTG TGGGGGTAAG TGCCCGACGA 
GGAGGCCTCG CCCCAACACA GTGTGTCAAG CTGGCTGGTG CCACCCACTA CCCGCTAGAC 
CGGTGCTGCT ATGGGAAATT CCTGCTGACG TCCCACAGGC TGGACCCCTG GAAGATGCAG 
GAAGCCTGGA AGCTGGTGGG TGGCTCTGAC GGGGAAAGGA CTGCGCCCTT TTGCTCCTGG 
TTCCAGGGAG TCCTGGGCAT CCTGCGGTGT CGAGCAATAG CCTGGCTTCA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Induction of GM1a/GD1b synthase triggers complex ganglioside expression and alters neuroblastoma cell behavior; a new tumor cell model of ganglioside function.
Glycoconjugate journal28(3-4)137-47(2011 May)
Dong L,Liu Y,Colberg-Poley AM,Kaucic K,Ladisch S

GM1?/?GD1b?/?GA1 synthase expression results in the reduced cancer phenotypes with modulation of composition and raft-localization of gangliosides in a melanoma cell line.
Cancer science101(9)2039-47(2010 Sep)
Dong Y,Ikeda K,Hamamura K,Zhang Q,Kondo Y,Matsumoto Y,Ohmi Y,Yamauchi Y,Furukawa K,Taguchi R,Furukawa K

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B3GALT4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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