ACTN1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017021722.1	XP_016877211.1	alpha-actinin-1 isoform X3
XM_011537267.2	XP_011535569.1	alpha-actinin-1 isoform X9
XM_017021721.2	XP_016877210.1	alpha-actinin-1 isoform X2
XM_017021726.2	XP_016877215.1	alpha-actinin-1 isoform X6
XM_017021728.2	XP_016877217.1	alpha-actinin-1 isoform X12
NM_001102.3	NP_001093.1	alpha-actinin-1 isoform b
XM_017021721.1	XP_016877210.1	alpha-actinin-1 isoform X2
XM_017021726.1	XP_016877215.1	alpha-actinin-1 isoform X6
XM_011537266.2	XP_011535568.1	alpha-actinin-1 isoform X8
XM_011537265.2	XP_011535567.1	alpha-actinin-1 isoform X7
XM_011537267.3	XP_011535569.1	alpha-actinin-1 isoform X9
NM_001102.4	NP_001093.1	alpha-actinin-1 isoform b
NM_001130004.1	NP_001123476.1	alpha-actinin-1 isoform a
XM_017021723.1	XP_016877212.1	alpha-actinin-1 isoform X4
XM_017021728.1	XP_016877217.1	alpha-actinin-1 isoform X12
XM_017021725.1	XP_016877214.1	alpha-actinin-1 isoform X5
XM_011537268.2	XP_011535570.1	alpha-actinin-1 isoform X10
XM_017021722.2	XP_016877211.1	alpha-actinin-1 isoform X3
XM_017021727.2	XP_016877216.1	alpha-actinin-1 isoform X11
XM_017021720.1	XP_016877209.1	alpha-actinin-1 isoform X1
NM_001130005.2	NP_001123477.1	alpha-actinin-1 isoform c
XM_017021727.1	XP_016877216.1	alpha-actinin-1 isoform X11
NM_001130005.1	NP_001123477.1	alpha-actinin-1 isoform c
XM_017021723.2	XP_016877212.1	alpha-actinin-1 isoform X4
XM_011537266.3	XP_011535568.1	alpha-actinin-1 isoform X8
XM_011537268.3	XP_011535570.1	alpha-actinin-1 isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10106	NM_001102.4 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu19389	NM_001130004.1 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu10093	NM_001130005.2 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$629.30 $899.00
OHu56130	XM_011537265.2 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu56131	XM_011537266.3 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu56132	XM_011537267.3 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu56133	XM_011537268.3 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu85145	XM_017021727.2 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu85146	XM_017021728.2 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu85139	XM_017021720.1 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu85140	XM_017021721.2 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu85141	XM_017021722.2 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu85142	XM_017021723.2 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu85143	XM_017021725.1 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu85144	XM_017021726.2 Latest version!	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OHu85140	XM_017021721.1	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu85144	XM_017021726.1	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu56132	XM_011537267.2	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu10106	NM_001102.3	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu85146	XM_017021728.1	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu85141	XM_017021722.1	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu85145	XM_017021727.1	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu10093	NM_001130005.1	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$629.30 $899.00
OHu85142	XM_017021723.1	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu56131	XM_011537266.2	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu56133	XM_011537268.2	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

ACTN1 ( NM_001102.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001093.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10106
Clone ID Related Accession (Same CDS sequence)	NM_001102.3 , NM_001102.4
Accession Version	NM_001102.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC321454.1, BC003576.1, BC004984.1 and BI494856.1. On Jul 11, 2008 this sequence version replaced NM_001102.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.167170.1, SRR1803612.50343.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGGAGAAGCA GAATTTGCCC GCATCATGAG CATTGTGGAC 
CCCAACCGCC TGGGGGTAGT GACATTCCAG GCCTTCATTG ACTTCATGTC CCGCGAGACA 
GCCGACACAG ATACAGCAGA CCAAGTCATG GCTTCCTTCA AGATCCTGGC TGGGGACAAG 
AACTACATTA CCATGGACGA GCTGCGCCGC GAGCTGCCAC CCGACCAGGC TGAGTACTGC 
ATCGCGCGGA TGGCCCCCTA CACCGGCCCC GACTCCGTGC CAGGTGCTCT GGACTACATG 
TCCTTCTCCA CGGCGCTGTA CGGCGAGAGT GACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093.1
CDS	311..2989
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	NM_001102.3
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102.3

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGGAGAAGCA GAATTTGCCC GCATCATGAG CATTGTGGAC 
CCCAACCGCC TGGGGGTAGT GACATTCCAG GCCTTCATTG ACTTCATGTC CCGCGAGACA 
GCCGACACAG ATACAGCAGA CCAAGTCATG GCTTCCTTCA AGATCCTGGC TGGGGACAAG 
AACTACATTA CCATGGACGA GCTGCGCCGC GAGCTGCCAC CCGACCAGGC TGAGTACTGC 
ATCGCGCGGA TGGCCCCCTA CACCGGCCCC GACTCCGTGC CAGGTGCTCT GGACTACATG 
TCCTTCTCCA CGGCGCTGTA CGGCGAGAGT GACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( NM_001102.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001093.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10106
Clone ID Related Accession (Same CDS sequence)	NM_001102.3 , NM_001102.4
Accession Version	NM_001102.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC321454.1, BC003576.1, BC004984.1 and BI494856.1. On Jun 2, 2019 this sequence version replaced NM_001102.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.50343.1, SRR1803611.71889.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGGAGAAGCA GAATTTGCCC GCATCATGAG CATTGTGGAC 
CCCAACCGCC TGGGGGTAGT GACATTCCAG GCCTTCATTG ACTTCATGTC CCGCGAGACA 
GCCGACACAG ATACAGCAGA CCAAGTCATG GCTTCCTTCA AGATCCTGGC TGGGGACAAG 
AACTACATTA CCATGGACGA GCTGCGCCGC GAGCTGCCAC CCGACCAGGC TGAGTACTGC 
ATCGCGCGGA TGGCCCCCTA CACCGGCCCC GACTCCGTGC CAGGTGCTCT GGACTACATG 
TCCTTCTCCA CGGCGCTGTA CGGCGAGAGT GACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093.1
CDS	247..2925
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	NM_001102.4
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102.4

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGGAGAAGCA GAATTTGCCC GCATCATGAG CATTGTGGAC 
CCCAACCGCC TGGGGGTAGT GACATTCCAG GCCTTCATTG ACTTCATGTC CCGCGAGACA 
GCCGACACAG ATACAGCAGA CCAAGTCATG GCTTCCTTCA AGATCCTGGC TGGGGACAAG 
AACTACATTA CCATGGACGA GCTGCGCCGC GAGCTGCCAC CCGACCAGGC TGAGTACTGC 
ATCGCGCGGA TGGCCCCCTA CACCGGCCCC GACTCCGTGC CAGGTGCTCT GGACTACATG 
TCCTTCTCCA CGGCGCTGTA CGGCGAGAGT GACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( NM_001130004.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123476.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant 1, mRNA.

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CloneID	OHu19389
Clone ID Related Accession (Same CDS sequence)	NM_001130004.1
Accession Version	NM_001130004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC372566.1, DQ496098.1, BC004984.1 and BI494856.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.235457.1, SRR1803617.86840.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC 
TGCCTGATCT CCATGGGTTA CAACATGGGA GAAGCAGAAT TTGCCCGCAT CATGAGCATT 
GTGGACCCCA ACCGCCTGGG GGTAGTGACA TTCCAGGCCT TCATTGACTT CATGTCCCGC 
GAGACAGCCG ACACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCTGGG 
GACAAGAACT ACATTACCAT GGACGAGCTG CGCCGCGAGC TGCCACCCGA CCAGGCTGAG 
TACTGCATCG CGCGGATGGC CCCCTACACC GGCCCCGACT CCGTGCCAGG TGCTCTGGAC 
TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123476.1
CDS	311..3055
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	NM_001130004.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130004.1

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC 
TGCCTGATCT CCATGGGTTA CAACATGGGA GAAGCAGAAT TTGCCCGCAT CATGAGCATT 
GTGGACCCCA ACCGCCTGGG GGTAGTGACA TTCCAGGCCT TCATTGACTT CATGTCCCGC 
GAGACAGCCG ACACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCTGGG 
GACAAGAACT ACATTACCAT GGACGAGCTG CGCCGCGAGC TGCCACCCGA CCAGGCTGAG 
TACTGCATCG CGCGGATGGC CCCCTACACC GGCCCCGACT CCGTGCCAGG TGCTCTGGAC 
TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( NM_001130005.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123477.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant 3, mRNA.

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CloneID	OHu10093
Clone ID Related Accession (Same CDS sequence)	NM_001130005.2 , NM_001130005.1
Accession Version	NM_001130005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC321454.1, EU716325.1, BC003576.1, BC004984.1 and BI494856.1. On Jun 2, 2019 this sequence version replaced NM_001130005.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.28986.1, SRR1660805.396.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTGATCTC CATGGGTTAC 
AACATGGGAG AAGCAGAATT TGCCCGCATC ATGAGCATTG TGGACCCCAA CCGCCTGGGG 
GTAGTGACAT TCCAGGCCTT CATTGACTTC ATGTCCCGCG AGACAGCCGA CACAGATACA 
GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCTGGGG ACAAGAACTA CATTACCATG 
GACGAGCTGC GCCGCGAGCT GCCACCCGAC CAGGCTGAGT ACTGCATCGC GCGGATGGCC 
CCCTACACCG GCCCCGACTC CGTGCCAGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG 
CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123477.1
CDS	247..2910
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	NM_001130005.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130005.2

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTGATCTC CATGGGTTAC 
AACATGGGAG AAGCAGAATT TGCCCGCATC ATGAGCATTG TGGACCCCAA CCGCCTGGGG 
GTAGTGACAT TCCAGGCCTT CATTGACTTC ATGTCCCGCG AGACAGCCGA CACAGATACA 
GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCTGGGG ACAAGAACTA CATTACCATG 
GACGAGCTGC GCCGCGAGCT GCCACCCGAC CAGGCTGAGT ACTGCATCGC GCGGATGGCC 
CCCTACACCG GCCCCGACTC CGTGCCAGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG 
CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( NM_001130005.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123477.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant 3, mRNA.

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CloneID	OHu10093
Clone ID Related Accession (Same CDS sequence)	NM_001130005.2 , NM_001130005.1
Accession Version	NM_001130005.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC321454.1, EU716325.1, BC003576.1, BC004984.1 and BI494856.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.23689.1, SRR1803612.172421.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTGATCTC CATGGGTTAC 
AACATGGGAG AAGCAGAATT TGCCCGCATC ATGAGCATTG TGGACCCCAA CCGCCTGGGG 
GTAGTGACAT TCCAGGCCTT CATTGACTTC ATGTCCCGCG AGACAGCCGA CACAGATACA 
GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCTGGGG ACAAGAACTA CATTACCATG 
GACGAGCTGC GCCGCGAGCT GCCACCCGAC CAGGCTGAGT ACTGCATCGC GCGGATGGCC 
CCCTACACCG GCCCCGACTC CGTGCCAGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG 
CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123477.1
CDS	311..2974
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	NM_001130005.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130005.1

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTGATCTC CATGGGTTAC 
AACATGGGAG AAGCAGAATT TGCCCGCATC ATGAGCATTG TGGACCCCAA CCGCCTGGGG 
GTAGTGACAT TCCAGGCCTT CATTGACTTC ATGTCCCGCG AGACAGCCGA CACAGATACA 
GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCTGGGG ACAAGAACTA CATTACCATG 
GACGAGCTGC GCCGCGAGCT GCCACCCGAC CAGGCTGAGT ACTGCATCGC GCGGATGGCC 
CCCTACACCG GCCCCGACTC CGTGCCAGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG 
CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_011537265.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535567.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X7, mRNA.

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CloneID	OHu56130
Clone ID Related Accession (Same CDS sequence)	XM_011537265.2
Accession Version	XM_011537265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GCCATGTGGC CCTCTCTCAA CCCAGGCAAA GAGGCCATGC TGCGACAGAA GGACTATGAG 
ACCGCCACCC TCTCGGAGAT CAAGGCCCTG CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC 
CTGGCTGCCC ACCAGGACCG TGTGGAGCAG ATTGCCGCCA TCGCACAGGA GCTCAATGAG 
CTGGACTATT ATGACTCACC CAGTGTCAAC GCCCGTTGCC AAAAGATCTG TGACCAGTGG 
GACAATCTGG GGGCCCTAAC TCAGAAGCGA AGGGAAGCTC TGGAGCGGAC CGAGAAACTG 
CTGGAGACCA TTGACCAGCT GTACTTGGAG TATGCCAAGC GGGCTGCACC CTTCAACAAC 
TGGATGGAGG GGGCCATGGA GGACCTGCAG GACACCTTCA TTGTGCACAC CATTGAGGAG 
ATCCAGGGAC TGACCACAGC CCATGAGCAG TTCAAGGCCA CCCTCCCTGA TGCCGACAAG 
GAGCGCCTGG CCATCCTGGG CATCCACAAT GAGGTGTCCA AGATTGTCCA GACCTACCAC 
GTCAATATGG CGGGCACCAA CCCCTACACA ACCATCACGC CTCAGGAGAT CAATGGCAAA 
TGGGACCACG TGCGGCAGCT GGTGCCTCGG AGGGACCAAG CTCTGACGGA GGAGCATGCC 
CGACAGCAGC ACAATGAGAG GCTACGCAAG CAGTTTGGAG CCCAGGCCAA TGTCATCGGG 
CCCTGGATCC AGACCAAGAT GGAGGAGATC GGGAGGATCT CCATTGAGAT GCATGGGACC 
CTGGAGGACC AGCTCAGCCA CCTGCGGCAG TATGAGAAGA GCATCGTCAA CTACAAGCCA 
AAGATTGATC AGCTGGAGGG CGACCACCAG CTCATCCAGG AGGCGCTCAT CTTCGACAAC 
AAGCACACCA ACTACACCAT GGAGCACATC CGTGTGGGCT GGGAGCAGCT GCTCACCACC 
ATCGCCAGGA CCATCAATGA GGTAGAGAAC CAGATCCTGA CCCGGGATGC CAAGGGCATC 
AGCCAGGAGC AGATGAATGA GTTCCGGGCC TCCTTCAACC ACTTTGACCG GGATCACTCC 
GGCACACTGG GTCCCGAGGA GTTCAAAGCC TGCCTCATCA GCTTGGGTTA TGATATTGGC 
AACGACCCCC AGGGAGAAGC AGAATTTGCC CGCATCATGA GCATTGTGGA CCCCAACCGC 
CTGGGGGTAG TGACATTCCA GGCCTTCATT GACTTCATGT CCCGCGAGAC AGCCGACACA 
GATACAGCAG ACCAAGTCAT GGCTTCCTTC AAGATCCTGG CTGGGGACAA GAACTACATT 
ACCATGGACG AGCTGCGCCG CGAGCTGCCA CCCGACCAGG CTGAGTACTG CATCGCGCGG 
ATGGCCCCCT ACACCGGCCC CGACTCCGTG CCAGGTGCTC TGGACTACAT GTCCTTCTCC 
ACGGCGCTGT ACGGCGAGAG TGACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535567.1
CDS	16..2805
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDAMWPSLNPGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_011537265.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537265.2

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GCCATGTGGC CCTCTCTCAA CCCAGGCAAA GAGGCCATGC TGCGACAGAA GGACTATGAG 
ACCGCCACCC TCTCGGAGAT CAAGGCCCTG CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC 
CTGGCTGCCC ACCAGGACCG TGTGGAGCAG ATTGCCGCCA TCGCACAGGA GCTCAATGAG 
CTGGACTATT ATGACTCACC CAGTGTCAAC GCCCGTTGCC AAAAGATCTG TGACCAGTGG 
GACAATCTGG GGGCCCTAAC TCAGAAGCGA AGGGAAGCTC TGGAGCGGAC CGAGAAACTG 
CTGGAGACCA TTGACCAGCT GTACTTGGAG TATGCCAAGC GGGCTGCACC CTTCAACAAC 
TGGATGGAGG GGGCCATGGA GGACCTGCAG GACACCTTCA TTGTGCACAC CATTGAGGAG 
ATCCAGGGAC TGACCACAGC CCATGAGCAG TTCAAGGCCA CCCTCCCTGA TGCCGACAAG 
GAGCGCCTGG CCATCCTGGG CATCCACAAT GAGGTGTCCA AGATTGTCCA GACCTACCAC 
GTCAATATGG CGGGCACCAA CCCCTACACA ACCATCACGC CTCAGGAGAT CAATGGCAAA 
TGGGACCACG TGCGGCAGCT GGTGCCTCGG AGGGACCAAG CTCTGACGGA GGAGCATGCC 
CGACAGCAGC ACAATGAGAG GCTACGCAAG CAGTTTGGAG CCCAGGCCAA TGTCATCGGG 
CCCTGGATCC AGACCAAGAT GGAGGAGATC GGGAGGATCT CCATTGAGAT GCATGGGACC 
CTGGAGGACC AGCTCAGCCA CCTGCGGCAG TATGAGAAGA GCATCGTCAA CTACAAGCCA 
AAGATTGATC AGCTGGAGGG CGACCACCAG CTCATCCAGG AGGCGCTCAT CTTCGACAAC 
AAGCACACCA ACTACACCAT GGAGCACATC CGTGTGGGCT GGGAGCAGCT GCTCACCACC 
ATCGCCAGGA CCATCAATGA GGTAGAGAAC CAGATCCTGA CCCGGGATGC CAAGGGCATC 
AGCCAGGAGC AGATGAATGA GTTCCGGGCC TCCTTCAACC ACTTTGACCG GGATCACTCC 
GGCACACTGG GTCCCGAGGA GTTCAAAGCC TGCCTCATCA GCTTGGGTTA TGATATTGGC 
AACGACCCCC AGGGAGAAGC AGAATTTGCC CGCATCATGA GCATTGTGGA CCCCAACCGC 
CTGGGGGTAG TGACATTCCA GGCCTTCATT GACTTCATGT CCCGCGAGAC AGCCGACACA 
GATACAGCAG ACCAAGTCAT GGCTTCCTTC AAGATCCTGG CTGGGGACAA GAACTACATT 
ACCATGGACG AGCTGCGCCG CGAGCTGCCA CCCGACCAGG CTGAGTACTG CATCGCGCGG 
ATGGCCCCCT ACACCGGCCC CGACTCCGTG CCAGGTGCTC TGGACTACAT GTCCTTCTCC 
ACGGCGCTGT ACGGCGAGAG TGACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_011537266.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535568.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu56131
Clone ID Related Accession (Same CDS sequence)	XM_011537266.2 , XM_011537266.3
Accession Version	XM_011537266.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GCCATGTGGC CCTCTCTCAA CCCAGGCAAA GAGGCCATGC TGCGACAGAA GGACTATGAG 
ACCGCCACCC TCTCGGAGAT CAAGGCCCTG CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC 
CTGGCTGCCC ACCAGGACCG TGTGGAGCAG ATTGCCGCCA TCGCACAGGA GCTCAATGAG 
CTGGACTATT ATGACTCACC CAGTGTCAAC GCCCGTTGCC AAAAGATCTG TGACCAGTGG 
GACAATCTGG GGGCCCTAAC TCAGAAGCGA AGGGAAGCTC TGGAGCGGAC CGAGAAACTG 
CTGGAGACCA TTGACCAGCT GTACTTGGAG TATGCCAAGC GGGCTGCACC CTTCAACAAC 
TGGATGGAGG GGGCCATGGA GGACCTGCAG GACACCTTCA TTGTGCACAC CATTGAGGAG 
ATCCAGGGAC TGACCACAGC CCATGAGCAG TTCAAGGCCA CCCTCCCTGA TGCCGACAAG 
GAGCGCCTGG CCATCCTGGG CATCCACAAT GAGGTGTCCA AGATTGTCCA GACCTACCAC 
GTCAATATGG CGGGCACCAA CCCCTACACA ACCATCACGC CTCAGGAGAT CAATGGCAAA 
TGGGACCACG TGCGGCAGCT GGTGCCTCGG AGGGACCAAG CTCTGACGGA GGAGCATGCC 
CGACAGCAGC ACAATGAGAG GCTACGCAAG CAGTTTGGAG CCCAGGCCAA TGTCATCGGG 
CCCTGGATCC AGACCAAGAT GGAGGAGATC GGGAGGATCT CCATTGAGAT GCATGGGACC 
CTGGAGGACC AGCTCAGCCA CCTGCGGCAG TATGAGAAGA GCATCGTCAA CTACAAGCCA 
AAGATTGATC AGCTGGAGGG CGACCACCAG CTCATCCAGG AGGCGCTCAT CTTCGACAAC 
AAGCACACCA ACTACACCAT GGAGCACATC CGTGTGGGCT GGGAGCAGCT GCTCACCACC 
ATCGCCAGGA CCATCAATGA GGTAGAGAAC CAGATCCTGA CCCGGGATGC CAAGGGCATC 
AGCCAGGAGC AGATGAATGA GTTCCGGGCC TCCTTCAACC ACTTTGACCG GAAGAAGACA 
GGCATGATGG ACACGGATGA TTTCCGCGCC TGCCTGATCT CCATGGGTTA CAACATGGGA 
GAAGCAGAAT TTGCCCGCAT CATGAGCATT GTGGACCCCA ACCGCCTGGG GGTAGTGACA 
TTCCAGGCCT TCATTGACTT CATGTCCCGC GAGACAGCCG ACACAGATAC AGCAGACCAA 
GTCATGGCTT CCTTCAAGAT CCTGGCTGGG GACAAGAACT ACATTACCAT GGACGAGCTG 
CGCCGCGAGC TGCCACCCGA CCAGGCTGAG TACTGCATCG CGCGGATGGC CCCCTACACC 
GGCCCCGACT CCGTGCCAGG TGCTCTGGAC TACATGTCCT TCTCCACGGC GCTGTACGGC 
GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535568.1
CDS	16..2790
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDAMWPSLNPGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_011537266.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537266.2

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GCCATGTGGC CCTCTCTCAA CCCAGGCAAA GAGGCCATGC TGCGACAGAA GGACTATGAG 
ACCGCCACCC TCTCGGAGAT CAAGGCCCTG CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC 
CTGGCTGCCC ACCAGGACCG TGTGGAGCAG ATTGCCGCCA TCGCACAGGA GCTCAATGAG 
CTGGACTATT ATGACTCACC CAGTGTCAAC GCCCGTTGCC AAAAGATCTG TGACCAGTGG 
GACAATCTGG GGGCCCTAAC TCAGAAGCGA AGGGAAGCTC TGGAGCGGAC CGAGAAACTG 
CTGGAGACCA TTGACCAGCT GTACTTGGAG TATGCCAAGC GGGCTGCACC CTTCAACAAC 
TGGATGGAGG GGGCCATGGA GGACCTGCAG GACACCTTCA TTGTGCACAC CATTGAGGAG 
ATCCAGGGAC TGACCACAGC CCATGAGCAG TTCAAGGCCA CCCTCCCTGA TGCCGACAAG 
GAGCGCCTGG CCATCCTGGG CATCCACAAT GAGGTGTCCA AGATTGTCCA GACCTACCAC 
GTCAATATGG CGGGCACCAA CCCCTACACA ACCATCACGC CTCAGGAGAT CAATGGCAAA 
TGGGACCACG TGCGGCAGCT GGTGCCTCGG AGGGACCAAG CTCTGACGGA GGAGCATGCC 
CGACAGCAGC ACAATGAGAG GCTACGCAAG CAGTTTGGAG CCCAGGCCAA TGTCATCGGG 
CCCTGGATCC AGACCAAGAT GGAGGAGATC GGGAGGATCT CCATTGAGAT GCATGGGACC 
CTGGAGGACC AGCTCAGCCA CCTGCGGCAG TATGAGAAGA GCATCGTCAA CTACAAGCCA 
AAGATTGATC AGCTGGAGGG CGACCACCAG CTCATCCAGG AGGCGCTCAT CTTCGACAAC 
AAGCACACCA ACTACACCAT GGAGCACATC CGTGTGGGCT GGGAGCAGCT GCTCACCACC 
ATCGCCAGGA CCATCAATGA GGTAGAGAAC CAGATCCTGA CCCGGGATGC CAAGGGCATC 
AGCCAGGAGC AGATGAATGA GTTCCGGGCC TCCTTCAACC ACTTTGACCG GAAGAAGACA 
GGCATGATGG ACACGGATGA TTTCCGCGCC TGCCTGATCT CCATGGGTTA CAACATGGGA 
GAAGCAGAAT TTGCCCGCAT CATGAGCATT GTGGACCCCA ACCGCCTGGG GGTAGTGACA 
TTCCAGGCCT TCATTGACTT CATGTCCCGC GAGACAGCCG ACACAGATAC AGCAGACCAA 
GTCATGGCTT CCTTCAAGAT CCTGGCTGGG GACAAGAACT ACATTACCAT GGACGAGCTG 
CGCCGCGAGC TGCCACCCGA CCAGGCTGAG TACTGCATCG CGCGGATGGC CCCCTACACC 
GGCCCCGACT CCGTGCCAGG TGCTCTGGAC TACATGTCCT TCTCCACGGC GCTGTACGGC 
GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_011537266.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535568.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu56131
Clone ID Related Accession (Same CDS sequence)	XM_011537266.2 , XM_011537266.3
Accession Version	XM_011537266.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011537266.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GCCATGTGGC CCTCTCTCAA CCCAGGCAAA GAGGCCATGC TGCGACAGAA GGACTATGAG 
ACCGCCACCC TCTCGGAGAT CAAGGCCCTG CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC 
CTGGCTGCCC ACCAGGACCG TGTGGAGCAG ATTGCCGCCA TCGCACAGGA GCTCAATGAG 
CTGGACTATT ATGACTCACC CAGTGTCAAC GCCCGTTGCC AAAAGATCTG TGACCAGTGG 
GACAATCTGG GGGCCCTAAC TCAGAAGCGA AGGGAAGCTC TGGAGCGGAC CGAGAAACTG 
CTGGAGACCA TTGACCAGCT GTACTTGGAG TATGCCAAGC GGGCTGCACC CTTCAACAAC 
TGGATGGAGG GGGCCATGGA GGACCTGCAG GACACCTTCA TTGTGCACAC CATTGAGGAG 
ATCCAGGGAC TGACCACAGC CCATGAGCAG TTCAAGGCCA CCCTCCCTGA TGCCGACAAG 
GAGCGCCTGG CCATCCTGGG CATCCACAAT GAGGTGTCCA AGATTGTCCA GACCTACCAC 
GTCAATATGG CGGGCACCAA CCCCTACACA ACCATCACGC CTCAGGAGAT CAATGGCAAA 
TGGGACCACG TGCGGCAGCT GGTGCCTCGG AGGGACCAAG CTCTGACGGA GGAGCATGCC 
CGACAGCAGC ACAATGAGAG GCTACGCAAG CAGTTTGGAG CCCAGGCCAA TGTCATCGGG 
CCCTGGATCC AGACCAAGAT GGAGGAGATC GGGAGGATCT CCATTGAGAT GCATGGGACC 
CTGGAGGACC AGCTCAGCCA CCTGCGGCAG TATGAGAAGA GCATCGTCAA CTACAAGCCA 
AAGATTGATC AGCTGGAGGG CGACCACCAG CTCATCCAGG AGGCGCTCAT CTTCGACAAC 
AAGCACACCA ACTACACCAT GGAGCACATC CGTGTGGGCT GGGAGCAGCT GCTCACCACC 
ATCGCCAGGA CCATCAATGA GGTAGAGAAC CAGATCCTGA CCCGGGATGC CAAGGGCATC 
AGCCAGGAGC AGATGAATGA GTTCCGGGCC TCCTTCAACC ACTTTGACCG GAAGAAGACA 
GGCATGATGG ACACGGATGA TTTCCGCGCC TGCCTGATCT CCATGGGTTA CAACATGGGA 
GAAGCAGAAT TTGCCCGCAT CATGAGCATT GTGGACCCCA ACCGCCTGGG GGTAGTGACA 
TTCCAGGCCT TCATTGACTT CATGTCCCGC GAGACAGCCG ACACAGATAC AGCAGACCAA 
GTCATGGCTT CCTTCAAGAT CCTGGCTGGG GACAAGAACT ACATTACCAT GGACGAGCTG 
CGCCGCGAGC TGCCACCCGA CCAGGCTGAG TACTGCATCG CGCGGATGGC CCCCTACACC 
GGCCCCGACT CCGTGCCAGG TGCTCTGGAC TACATGTCCT TCTCCACGGC GCTGTACGGC 
GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535568.1
CDS	16..2790
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDAMWPSLNPGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_011537266.3
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537266.3

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GCCATGTGGC CCTCTCTCAA CCCAGGCAAA GAGGCCATGC TGCGACAGAA GGACTATGAG 
ACCGCCACCC TCTCGGAGAT CAAGGCCCTG CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC 
CTGGCTGCCC ACCAGGACCG TGTGGAGCAG ATTGCCGCCA TCGCACAGGA GCTCAATGAG 
CTGGACTATT ATGACTCACC CAGTGTCAAC GCCCGTTGCC AAAAGATCTG TGACCAGTGG 
GACAATCTGG GGGCCCTAAC TCAGAAGCGA AGGGAAGCTC TGGAGCGGAC CGAGAAACTG 
CTGGAGACCA TTGACCAGCT GTACTTGGAG TATGCCAAGC GGGCTGCACC CTTCAACAAC 
TGGATGGAGG GGGCCATGGA GGACCTGCAG GACACCTTCA TTGTGCACAC CATTGAGGAG 
ATCCAGGGAC TGACCACAGC CCATGAGCAG TTCAAGGCCA CCCTCCCTGA TGCCGACAAG 
GAGCGCCTGG CCATCCTGGG CATCCACAAT GAGGTGTCCA AGATTGTCCA GACCTACCAC 
GTCAATATGG CGGGCACCAA CCCCTACACA ACCATCACGC CTCAGGAGAT CAATGGCAAA 
TGGGACCACG TGCGGCAGCT GGTGCCTCGG AGGGACCAAG CTCTGACGGA GGAGCATGCC 
CGACAGCAGC ACAATGAGAG GCTACGCAAG CAGTTTGGAG CCCAGGCCAA TGTCATCGGG 
CCCTGGATCC AGACCAAGAT GGAGGAGATC GGGAGGATCT CCATTGAGAT GCATGGGACC 
CTGGAGGACC AGCTCAGCCA CCTGCGGCAG TATGAGAAGA GCATCGTCAA CTACAAGCCA 
AAGATTGATC AGCTGGAGGG CGACCACCAG CTCATCCAGG AGGCGCTCAT CTTCGACAAC 
AAGCACACCA ACTACACCAT GGAGCACATC CGTGTGGGCT GGGAGCAGCT GCTCACCACC 
ATCGCCAGGA CCATCAATGA GGTAGAGAAC CAGATCCTGA CCCGGGATGC CAAGGGCATC 
AGCCAGGAGC AGATGAATGA GTTCCGGGCC TCCTTCAACC ACTTTGACCG GAAGAAGACA 
GGCATGATGG ACACGGATGA TTTCCGCGCC TGCCTGATCT CCATGGGTTA CAACATGGGA 
GAAGCAGAAT TTGCCCGCAT CATGAGCATT GTGGACCCCA ACCGCCTGGG GGTAGTGACA 
TTCCAGGCCT TCATTGACTT CATGTCCCGC GAGACAGCCG ACACAGATAC AGCAGACCAA 
GTCATGGCTT CCTTCAAGAT CCTGGCTGGG GACAAGAACT ACATTACCAT GGACGAGCTG 
CGCCGCGAGC TGCCACCCGA CCAGGCTGAG TACTGCATCG CGCGGATGGC CCCCTACACC 
GGCCCCGACT CCGTGCCAGG TGCTCTGGAC TACATGTCCT TCTCCACGGC GCTGTACGGC 
GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_011537267.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535569.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu56132
Clone ID Related Accession (Same CDS sequence)	XM_011537267.2 , XM_011537267.3
Accession Version	XM_011537267.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGGAT CACTCCGGCA CACTGGGTCC CGAGGAGTTC 
AAAGCCTGCC TCATCAGCTT GGGTTATGAT ATTGGCAACG ACCCCCAGGG AGAAGCAGAA 
TTTGCCCGCA TCATGAGCAT TGTGGACCCC AACCGCCTGG GGGTAGTGAC ATTCCAGGCC 
TTCATTGACT TCATGTCCCG CGAGACAGCC GACACAGATA CAGCAGACCA AGTCATGGCT 
TCCTTCAAGA TCCTGGCTGG GGACAAGAAC TACATTACCA TGGACGAGCT GCGCCGCGAG 
CTGCCACCCG ACCAGGCTGA GTACTGCATC GCGCGGATGG CCCCCTACAC CGGCCCCGAC 
TCCGTGCCAG GTGCTCTGGA CTACATGTCC TTCTCCACGG CGCTGTACGG CGAGAGTGAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535569.1
CDS	16..2781
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_011537267.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537267.2

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGGAT CACTCCGGCA CACTGGGTCC CGAGGAGTTC 
AAAGCCTGCC TCATCAGCTT GGGTTATGAT ATTGGCAACG ACCCCCAGGG AGAAGCAGAA 
TTTGCCCGCA TCATGAGCAT TGTGGACCCC AACCGCCTGG GGGTAGTGAC ATTCCAGGCC 
TTCATTGACT TCATGTCCCG CGAGACAGCC GACACAGATA CAGCAGACCA AGTCATGGCT 
TCCTTCAAGA TCCTGGCTGG GGACAAGAAC TACATTACCA TGGACGAGCT GCGCCGCGAG 
CTGCCACCCG ACCAGGCTGA GTACTGCATC GCGCGGATGG CCCCCTACAC CGGCCCCGAC 
TCCGTGCCAG GTGCTCTGGA CTACATGTCC TTCTCCACGG CGCTGTACGG CGAGAGTGAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_011537267.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535569.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu56132
Clone ID Related Accession (Same CDS sequence)	XM_011537267.2 , XM_011537267.3
Accession Version	XM_011537267.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011537267.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGGAT CACTCCGGCA CACTGGGTCC CGAGGAGTTC 
AAAGCCTGCC TCATCAGCTT GGGTTATGAT ATTGGCAACG ACCCCCAGGG AGAAGCAGAA 
TTTGCCCGCA TCATGAGCAT TGTGGACCCC AACCGCCTGG GGGTAGTGAC ATTCCAGGCC 
TTCATTGACT TCATGTCCCG CGAGACAGCC GACACAGATA CAGCAGACCA AGTCATGGCT 
TCCTTCAAGA TCCTGGCTGG GGACAAGAAC TACATTACCA TGGACGAGCT GCGCCGCGAG 
CTGCCACCCG ACCAGGCTGA GTACTGCATC GCGCGGATGG CCCCCTACAC CGGCCCCGAC 
TCCGTGCCAG GTGCTCTGGA CTACATGTCC TTCTCCACGG CGCTGTACGG CGAGAGTGAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535569.1
CDS	16..2781
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_011537267.3
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537267.3

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGGAT CACTCCGGCA CACTGGGTCC CGAGGAGTTC 
AAAGCCTGCC TCATCAGCTT GGGTTATGAT ATTGGCAACG ACCCCCAGGG AGAAGCAGAA 
TTTGCCCGCA TCATGAGCAT TGTGGACCCC AACCGCCTGG GGGTAGTGAC ATTCCAGGCC 
TTCATTGACT TCATGTCCCG CGAGACAGCC GACACAGATA CAGCAGACCA AGTCATGGCT 
TCCTTCAAGA TCCTGGCTGG GGACAAGAAC TACATTACCA TGGACGAGCT GCGCCGCGAG 
CTGCCACCCG ACCAGGCTGA GTACTGCATC GCGCGGATGG CCCCCTACAC CGGCCCCGAC 
TCCGTGCCAG GTGCTCTGGA CTACATGTCC TTCTCCACGG CGCTGTACGG CGAGAGTGAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_011537268.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535570.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu56133
Clone ID Related Accession (Same CDS sequence)	XM_011537268.2 , XM_011537268.3
Accession Version	XM_011537268.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGAAG AAGACAGGCA TGATGGACAC GGATGATTTC 
CGCGCCTGCC TGATCTCCAT GGGTTACAAC ATGGGAGAAG CAGAATTTGC CCGCATCATG 
AGCATTGTGG ACCCCAACCG CCTGGGGGTA GTGACATTCC AGGCCTTCAT TGACTTCATG 
TCCCGCGAGA CAGCCGACAC AGATACAGCA GACCAAGTCA TGGCTTCCTT CAAGATCCTG 
GCTGGGGACA AGAACTACAT TACCATGGAC GAGCTGCGCC GCGAGCTGCC ACCCGACCAG 
GCTGAGTACT GCATCGCGCG GATGGCCCCC TACACCGGCC CCGACTCCGT GCCAGGTGCT 
CTGGACTACA TGTCCTTCTC CACGGCGCTG TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535570.1
CDS	16..2766
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_011537268.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537268.2

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGAAG AAGACAGGCA TGATGGACAC GGATGATTTC 
CGCGCCTGCC TGATCTCCAT GGGTTACAAC ATGGGAGAAG CAGAATTTGC CCGCATCATG 
AGCATTGTGG ACCCCAACCG CCTGGGGGTA GTGACATTCC AGGCCTTCAT TGACTTCATG 
TCCCGCGAGA CAGCCGACAC AGATACAGCA GACCAAGTCA TGGCTTCCTT CAAGATCCTG 
GCTGGGGACA AGAACTACAT TACCATGGAC GAGCTGCGCC GCGAGCTGCC ACCCGACCAG 
GCTGAGTACT GCATCGCGCG GATGGCCCCC TACACCGGCC CCGACTCCGT GCCAGGTGCT 
CTGGACTACA TGTCCTTCTC CACGGCGCTG TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_011537268.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535570.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu56133
Clone ID Related Accession (Same CDS sequence)	XM_011537268.2 , XM_011537268.3
Accession Version	XM_011537268.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011537268.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGAAG AAGACAGGCA TGATGGACAC GGATGATTTC 
CGCGCCTGCC TGATCTCCAT GGGTTACAAC ATGGGAGAAG CAGAATTTGC CCGCATCATG 
AGCATTGTGG ACCCCAACCG CCTGGGGGTA GTGACATTCC AGGCCTTCAT TGACTTCATG 
TCCCGCGAGA CAGCCGACAC AGATACAGCA GACCAAGTCA TGGCTTCCTT CAAGATCCTG 
GCTGGGGACA AGAACTACAT TACCATGGAC GAGCTGCGCC GCGAGCTGCC ACCCGACCAG 
GCTGAGTACT GCATCGCGCG GATGGCCCCC TACACCGGCC CCGACTCCGT GCCAGGTGCT 
CTGGACTACA TGTCCTTCTC CACGGCGCTG TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535570.1
CDS	16..2766
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_011537268.3
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537268.3

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGAAG AAGACAGGCA TGATGGACAC GGATGATTTC 
CGCGCCTGCC TGATCTCCAT GGGTTACAAC ATGGGAGAAG CAGAATTTGC CCGCATCATG 
AGCATTGTGG ACCCCAACCG CCTGGGGGTA GTGACATTCC AGGCCTTCAT TGACTTCATG 
TCCCGCGAGA CAGCCGACAC AGATACAGCA GACCAAGTCA TGGCTTCCTT CAAGATCCTG 
GCTGGGGACA AGAACTACAT TACCATGGAC GAGCTGCGCC GCGAGCTGCC ACCCGACCAG 
GCTGAGTACT GCATCGCGCG GATGGCCCCC TACACCGGCC CCGACTCCGT GCCAGGTGCT 
CTGGACTACA TGTCCTTCTC CACGGCGCTG TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021727.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877216.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu85145
Clone ID Related Accession (Same CDS sequence)	XM_017021727.2 , XM_017021727.1
Accession Version	XM_017021727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGCCATGT GGCCCTCTCT CAACCCAGGC 
AAAGAGGCCA TGCTGCGACA GAAGGACTAT GAGACCGCCA CCCTCTCGGA GATCAAGGCC 
CTGCTCAAGA AGCATGAGGC CTTCGAGAGT GACCTGGCTG CCCACCAGGA CCGTGTGGAG 
CAGATTGCCG CCATCGCACA GGAGCTCAAT GAGCTGGACT ATTATGACTC ACCCAGTGTC 
AACGCCCGTT GCCAAAAGAT CTGTGACCAG TGGGACAATC TGGGGGCCCT AACTCAGAAG 
CGAAGGGAAG CTCTGGAGCG GACCGAGAAA CTGCTGGAGA CCATTGACCA GCTGTACTTG 
GAGTATGCCA AGCGGGCTGC ACCCTTCAAC AACTGGATGG AGGGGGCCAT GGAGGACCTG 
CAGGACACCT TCATTGTGCA CACCATTGAG GAGATCCAGG GACTGACCAC AGCCCATGAG 
CAGTTCAAGG CCACCCTCCC TGATGCCGAC AAGGAGCGCC TGGCCATCCT GGGCATCCAC 
AATGAGGTGT CCAAGATTGT CCAGACCTAC CACGTCAATA TGGCGGGCAC CAACCCCTAC 
ACAACCATCA CGCCTCAGGA GATCAATGGC AAATGGGACC ACGTGCGGCA GCTGGTGCCT 
CGGAGGGACC AAGCTCTGAC GGAGGAGCAT GCCCGACAGC AGCACAATGA GAGGCTACGC 
AAGCAGTTTG GAGCCCAGGC CAATGTCATC GGGCCCTGGA TCCAGACCAA GATGGAGGAG 
ATCGGGAGGA TCTCCATTGA GATGCATGGG ACCCTGGAGG ACCAGCTCAG CCACCTGCGG 
CAGTATGAGA AGAGCATCGT CAACTACAAG CCAAAGATTG ATCAGCTGGA GGGCGACCAC 
CAGCTCATCC AGGAGGCGCT CATCTTCGAC AACAAGCACA CCAACTACAC CATGGAGCAC 
ATCCGTGTGG GCTGGGAGCA GCTGCTCACC ACCATCGCCA GGACCATCAA TGAGGTAGAG 
AACCAGATCC TGACCCGGGA TGCCAAGGGC ATCAGCCAGG AGCAGATGAA TGAGTTCCGG 
GCCTCCTTCA ACCACTTTGA CCGGAAGAAG ACAGGCATGA TGGACACGGA TGATTTCCGC 
GCCTGCCTGA TCTCCATGGG TTACAACATG GGAGAAGCAG AATTTGCCCG CATCATGAGC 
ATTGTGGACC CCAACCGCCT GGGGGTAGTG ACATTCCAGG CCTTCATTGA CTTCATGTCC 
CGCGAGACAG CCGACACAGA TACAGCAGAC CAAGTCATGG CTTCCTTCAA GATCCTGGCT 
GGGGACAAGA ACTACATTAC CATGGACGAG CTGCGCCGCG AGCTGCCACC CGACCAGGCT 
GAGTACTGCA TCGCGCGGAT GGCCCCCTAC ACCGGCCCCG ACTCCGTGCC AGGTGCTCTG 
GACTACATGT CCTTCTCCAC GGCGCTGTAC GGCGAGAGTG ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877216.1
CDS	237..2924
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDAMWPSLNPGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021727.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021727.2

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGCCATGT GGCCCTCTCT CAACCCAGGC 
AAAGAGGCCA TGCTGCGACA GAAGGACTAT GAGACCGCCA CCCTCTCGGA GATCAAGGCC 
CTGCTCAAGA AGCATGAGGC CTTCGAGAGT GACCTGGCTG CCCACCAGGA CCGTGTGGAG 
CAGATTGCCG CCATCGCACA GGAGCTCAAT GAGCTGGACT ATTATGACTC ACCCAGTGTC 
AACGCCCGTT GCCAAAAGAT CTGTGACCAG TGGGACAATC TGGGGGCCCT AACTCAGAAG 
CGAAGGGAAG CTCTGGAGCG GACCGAGAAA CTGCTGGAGA CCATTGACCA GCTGTACTTG 
GAGTATGCCA AGCGGGCTGC ACCCTTCAAC AACTGGATGG AGGGGGCCAT GGAGGACCTG 
CAGGACACCT TCATTGTGCA CACCATTGAG GAGATCCAGG GACTGACCAC AGCCCATGAG 
CAGTTCAAGG CCACCCTCCC TGATGCCGAC AAGGAGCGCC TGGCCATCCT GGGCATCCAC 
AATGAGGTGT CCAAGATTGT CCAGACCTAC CACGTCAATA TGGCGGGCAC CAACCCCTAC 
ACAACCATCA CGCCTCAGGA GATCAATGGC AAATGGGACC ACGTGCGGCA GCTGGTGCCT 
CGGAGGGACC AAGCTCTGAC GGAGGAGCAT GCCCGACAGC AGCACAATGA GAGGCTACGC 
AAGCAGTTTG GAGCCCAGGC CAATGTCATC GGGCCCTGGA TCCAGACCAA GATGGAGGAG 
ATCGGGAGGA TCTCCATTGA GATGCATGGG ACCCTGGAGG ACCAGCTCAG CCACCTGCGG 
CAGTATGAGA AGAGCATCGT CAACTACAAG CCAAAGATTG ATCAGCTGGA GGGCGACCAC 
CAGCTCATCC AGGAGGCGCT CATCTTCGAC AACAAGCACA CCAACTACAC CATGGAGCAC 
ATCCGTGTGG GCTGGGAGCA GCTGCTCACC ACCATCGCCA GGACCATCAA TGAGGTAGAG 
AACCAGATCC TGACCCGGGA TGCCAAGGGC ATCAGCCAGG AGCAGATGAA TGAGTTCCGG 
GCCTCCTTCA ACCACTTTGA CCGGAAGAAG ACAGGCATGA TGGACACGGA TGATTTCCGC 
GCCTGCCTGA TCTCCATGGG TTACAACATG GGAGAAGCAG AATTTGCCCG CATCATGAGC 
ATTGTGGACC CCAACCGCCT GGGGGTAGTG ACATTCCAGG CCTTCATTGA CTTCATGTCC 
CGCGAGACAG CCGACACAGA TACAGCAGAC CAAGTCATGG CTTCCTTCAA GATCCTGGCT 
GGGGACAAGA ACTACATTAC CATGGACGAG CTGCGCCGCG AGCTGCCACC CGACCAGGCT 
GAGTACTGCA TCGCGCGGAT GGCCCCCTAC ACCGGCCCCG ACTCCGTGCC AGGTGCTCTG 
GACTACATGT CCTTCTCCAC GGCGCTGTAC GGCGAGAGTG ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021727.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877216.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu85145
Clone ID Related Accession (Same CDS sequence)	XM_017021727.2 , XM_017021727.1
Accession Version	XM_017021727.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGCCATGT GGCCCTCTCT CAACCCAGGC 
AAAGAGGCCA TGCTGCGACA GAAGGACTAT GAGACCGCCA CCCTCTCGGA GATCAAGGCC 
CTGCTCAAGA AGCATGAGGC CTTCGAGAGT GACCTGGCTG CCCACCAGGA CCGTGTGGAG 
CAGATTGCCG CCATCGCACA GGAGCTCAAT GAGCTGGACT ATTATGACTC ACCCAGTGTC 
AACGCCCGTT GCCAAAAGAT CTGTGACCAG TGGGACAATC TGGGGGCCCT AACTCAGAAG 
CGAAGGGAAG CTCTGGAGCG GACCGAGAAA CTGCTGGAGA CCATTGACCA GCTGTACTTG 
GAGTATGCCA AGCGGGCTGC ACCCTTCAAC AACTGGATGG AGGGGGCCAT GGAGGACCTG 
CAGGACACCT TCATTGTGCA CACCATTGAG GAGATCCAGG GACTGACCAC AGCCCATGAG 
CAGTTCAAGG CCACCCTCCC TGATGCCGAC AAGGAGCGCC TGGCCATCCT GGGCATCCAC 
AATGAGGTGT CCAAGATTGT CCAGACCTAC CACGTCAATA TGGCGGGCAC CAACCCCTAC 
ACAACCATCA CGCCTCAGGA GATCAATGGC AAATGGGACC ACGTGCGGCA GCTGGTGCCT 
CGGAGGGACC AAGCTCTGAC GGAGGAGCAT GCCCGACAGC AGCACAATGA GAGGCTACGC 
AAGCAGTTTG GAGCCCAGGC CAATGTCATC GGGCCCTGGA TCCAGACCAA GATGGAGGAG 
ATCGGGAGGA TCTCCATTGA GATGCATGGG ACCCTGGAGG ACCAGCTCAG CCACCTGCGG 
CAGTATGAGA AGAGCATCGT CAACTACAAG CCAAAGATTG ATCAGCTGGA GGGCGACCAC 
CAGCTCATCC AGGAGGCGCT CATCTTCGAC AACAAGCACA CCAACTACAC CATGGAGCAC 
ATCCGTGTGG GCTGGGAGCA GCTGCTCACC ACCATCGCCA GGACCATCAA TGAGGTAGAG 
AACCAGATCC TGACCCGGGA TGCCAAGGGC ATCAGCCAGG AGCAGATGAA TGAGTTCCGG 
GCCTCCTTCA ACCACTTTGA CCGGAAGAAG ACAGGCATGA TGGACACGGA TGATTTCCGC 
GCCTGCCTGA TCTCCATGGG TTACAACATG GGAGAAGCAG AATTTGCCCG CATCATGAGC 
ATTGTGGACC CCAACCGCCT GGGGGTAGTG ACATTCCAGG CCTTCATTGA CTTCATGTCC 
CGCGAGACAG CCGACACAGA TACAGCAGAC CAAGTCATGG CTTCCTTCAA GATCCTGGCT 
GGGGACAAGA ACTACATTAC CATGGACGAG CTGCGCCGCG AGCTGCCACC CGACCAGGCT 
GAGTACTGCA TCGCGCGGAT GGCCCCCTAC ACCGGCCCCG ACTCCGTGCC AGGTGCTCTG 
GACTACATGT CCTTCTCCAC GGCGCTGTAC GGCGAGAGTG ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877216.1
CDS	248..2935
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDAMWPSLNPGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021727.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021727.1

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGCCATGT GGCCCTCTCT CAACCCAGGC 
AAAGAGGCCA TGCTGCGACA GAAGGACTAT GAGACCGCCA CCCTCTCGGA GATCAAGGCC 
CTGCTCAAGA AGCATGAGGC CTTCGAGAGT GACCTGGCTG CCCACCAGGA CCGTGTGGAG 
CAGATTGCCG CCATCGCACA GGAGCTCAAT GAGCTGGACT ATTATGACTC ACCCAGTGTC 
AACGCCCGTT GCCAAAAGAT CTGTGACCAG TGGGACAATC TGGGGGCCCT AACTCAGAAG 
CGAAGGGAAG CTCTGGAGCG GACCGAGAAA CTGCTGGAGA CCATTGACCA GCTGTACTTG 
GAGTATGCCA AGCGGGCTGC ACCCTTCAAC AACTGGATGG AGGGGGCCAT GGAGGACCTG 
CAGGACACCT TCATTGTGCA CACCATTGAG GAGATCCAGG GACTGACCAC AGCCCATGAG 
CAGTTCAAGG CCACCCTCCC TGATGCCGAC AAGGAGCGCC TGGCCATCCT GGGCATCCAC 
AATGAGGTGT CCAAGATTGT CCAGACCTAC CACGTCAATA TGGCGGGCAC CAACCCCTAC 
ACAACCATCA CGCCTCAGGA GATCAATGGC AAATGGGACC ACGTGCGGCA GCTGGTGCCT 
CGGAGGGACC AAGCTCTGAC GGAGGAGCAT GCCCGACAGC AGCACAATGA GAGGCTACGC 
AAGCAGTTTG GAGCCCAGGC CAATGTCATC GGGCCCTGGA TCCAGACCAA GATGGAGGAG 
ATCGGGAGGA TCTCCATTGA GATGCATGGG ACCCTGGAGG ACCAGCTCAG CCACCTGCGG 
CAGTATGAGA AGAGCATCGT CAACTACAAG CCAAAGATTG ATCAGCTGGA GGGCGACCAC 
CAGCTCATCC AGGAGGCGCT CATCTTCGAC AACAAGCACA CCAACTACAC CATGGAGCAC 
ATCCGTGTGG GCTGGGAGCA GCTGCTCACC ACCATCGCCA GGACCATCAA TGAGGTAGAG 
AACCAGATCC TGACCCGGGA TGCCAAGGGC ATCAGCCAGG AGCAGATGAA TGAGTTCCGG 
GCCTCCTTCA ACCACTTTGA CCGGAAGAAG ACAGGCATGA TGGACACGGA TGATTTCCGC 
GCCTGCCTGA TCTCCATGGG TTACAACATG GGAGAAGCAG AATTTGCCCG CATCATGAGC 
ATTGTGGACC CCAACCGCCT GGGGGTAGTG ACATTCCAGG CCTTCATTGA CTTCATGTCC 
CGCGAGACAG CCGACACAGA TACAGCAGAC CAAGTCATGG CTTCCTTCAA GATCCTGGCT 
GGGGACAAGA ACTACATTAC CATGGACGAG CTGCGCCGCG AGCTGCCACC CGACCAGGCT 
GAGTACTGCA TCGCGCGGAT GGCCCCCTAC ACCGGCCCCG ACTCCGTGCC AGGTGCTCTG 
GACTACATGT CCTTCTCCAC GGCGCTGTAC GGCGAGAGTG ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021728.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877217.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu85146
Clone ID Related Accession (Same CDS sequence)	XM_017021728.2 , XM_017021728.1
Accession Version	XM_017021728.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GGCAAAGAGG CCATGCTGCG ACAGAAGGAC 
TATGAGACCG CCACCCTCTC GGAGATCAAG GCCCTGCTCA AGAAGCATGA GGCCTTCGAG 
AGTGACCTGG CTGCCCACCA GGACCGTGTG GAGCAGATTG CCGCCATCGC ACAGGAGCTC 
AATGAGCTGG ACTATTATGA CTCACCCAGT GTCAACGCCC GTTGCCAAAA GATCTGTGAC 
CAGTGGGACA ATCTGGGGGC CCTAACTCAG AAGCGAAGGG AAGCTCTGGA GCGGACCGAG 
AAACTGCTGG AGACCATTGA CCAGCTGTAC TTGGAGTATG CCAAGCGGGC TGCACCCTTC 
AACAACTGGA TGGAGGGGGC CATGGAGGAC CTGCAGGACA CCTTCATTGT GCACACCATT 
GAGGAGATCC AGGGACTGAC CACAGCCCAT GAGCAGTTCA AGGCCACCCT CCCTGATGCC 
GACAAGGAGC GCCTGGCCAT CCTGGGCATC CACAATGAGG TGTCCAAGAT TGTCCAGACC 
TACCACGTCA ATATGGCGGG CACCAACCCC TACACAACCA TCACGCCTCA GGAGATCAAT 
GGCAAATGGG ACCACGTGCG GCAGCTGGTG CCTCGGAGGG ACCAAGCTCT GACGGAGGAG 
CATGCCCGAC AGCAGCACAA TGAGAGGCTA CGCAAGCAGT TTGGAGCCCA GGCCAATGTC 
ATCGGGCCCT GGATCCAGAC CAAGATGGAG GAGATCGGGA GGATCTCCAT TGAGATGCAT 
GGGACCCTGG AGGACCAGCT CAGCCACCTG CGGCAGTATG AGAAGAGCAT CGTCAACTAC 
AAGCCAAAGA TTGATCAGCT GGAGGGCGAC CACCAGCTCA TCCAGGAGGC GCTCATCTTC 
GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATCG CCAGGACCAT CAATGAGGTA GAGAACCAGA TCCTGACCCG GGATGCCAAG 
GGCATCAGCC AGGAGCAGAT GAATGAGTTC CGGGCCTCCT TCAACCACTT TGACCGGAAG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TGATCTCCAT GGGTTACAAC 
ATGGGAGAAG CAGAATTTGC CCGCATCATG AGCATTGTGG ACCCCAACCG CCTGGGGGTA 
GTGACATTCC AGGCCTTCAT TGACTTCATG TCCCGCGAGA CAGCCGACAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCTGGGGACA AGAACTACAT TACCATGGAC 
GAGCTGCGCC GCGAGCTGCC ACCCGACCAG GCTGAGTACT GCATCGCGCG GATGGCCCCC 
TACACCGGCC CCGACTCCGT GCCAGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877217.1
CDS	485..3085
Translation	MGLRYVLPLRPPGKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021728.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021728.2

ORF Insert Sequence:

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GGCAAAGAGG CCATGCTGCG ACAGAAGGAC 
TATGAGACCG CCACCCTCTC GGAGATCAAG GCCCTGCTCA AGAAGCATGA GGCCTTCGAG 
AGTGACCTGG CTGCCCACCA GGACCGTGTG GAGCAGATTG CCGCCATCGC ACAGGAGCTC 
AATGAGCTGG ACTATTATGA CTCACCCAGT GTCAACGCCC GTTGCCAAAA GATCTGTGAC 
CAGTGGGACA ATCTGGGGGC CCTAACTCAG AAGCGAAGGG AAGCTCTGGA GCGGACCGAG 
AAACTGCTGG AGACCATTGA CCAGCTGTAC TTGGAGTATG CCAAGCGGGC TGCACCCTTC 
AACAACTGGA TGGAGGGGGC CATGGAGGAC CTGCAGGACA CCTTCATTGT GCACACCATT 
GAGGAGATCC AGGGACTGAC CACAGCCCAT GAGCAGTTCA AGGCCACCCT CCCTGATGCC 
GACAAGGAGC GCCTGGCCAT CCTGGGCATC CACAATGAGG TGTCCAAGAT TGTCCAGACC 
TACCACGTCA ATATGGCGGG CACCAACCCC TACACAACCA TCACGCCTCA GGAGATCAAT 
GGCAAATGGG ACCACGTGCG GCAGCTGGTG CCTCGGAGGG ACCAAGCTCT GACGGAGGAG 
CATGCCCGAC AGCAGCACAA TGAGAGGCTA CGCAAGCAGT TTGGAGCCCA GGCCAATGTC 
ATCGGGCCCT GGATCCAGAC CAAGATGGAG GAGATCGGGA GGATCTCCAT TGAGATGCAT 
GGGACCCTGG AGGACCAGCT CAGCCACCTG CGGCAGTATG AGAAGAGCAT CGTCAACTAC 
AAGCCAAAGA TTGATCAGCT GGAGGGCGAC CACCAGCTCA TCCAGGAGGC GCTCATCTTC 
GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATCG CCAGGACCAT CAATGAGGTA GAGAACCAGA TCCTGACCCG GGATGCCAAG 
GGCATCAGCC AGGAGCAGAT GAATGAGTTC CGGGCCTCCT TCAACCACTT TGACCGGAAG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TGATCTCCAT GGGTTACAAC 
ATGGGAGAAG CAGAATTTGC CCGCATCATG AGCATTGTGG ACCCCAACCG CCTGGGGGTA 
GTGACATTCC AGGCCTTCAT TGACTTCATG TCCCGCGAGA CAGCCGACAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCTGGGGACA AGAACTACAT TACCATGGAC 
GAGCTGCGCC GCGAGCTGCC ACCCGACCAG GCTGAGTACT GCATCGCGCG GATGGCCCCC 
TACACCGGCC CCGACTCCGT GCCAGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021728.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877217.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu85146
Clone ID Related Accession (Same CDS sequence)	XM_017021728.2 , XM_017021728.1
Accession Version	XM_017021728.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GGCAAAGAGG CCATGCTGCG ACAGAAGGAC 
TATGAGACCG CCACCCTCTC GGAGATCAAG GCCCTGCTCA AGAAGCATGA GGCCTTCGAG 
AGTGACCTGG CTGCCCACCA GGACCGTGTG GAGCAGATTG CCGCCATCGC ACAGGAGCTC 
AATGAGCTGG ACTATTATGA CTCACCCAGT GTCAACGCCC GTTGCCAAAA GATCTGTGAC 
CAGTGGGACA ATCTGGGGGC CCTAACTCAG AAGCGAAGGG AAGCTCTGGA GCGGACCGAG 
AAACTGCTGG AGACCATTGA CCAGCTGTAC TTGGAGTATG CCAAGCGGGC TGCACCCTTC 
AACAACTGGA TGGAGGGGGC CATGGAGGAC CTGCAGGACA CCTTCATTGT GCACACCATT 
GAGGAGATCC AGGGACTGAC CACAGCCCAT GAGCAGTTCA AGGCCACCCT CCCTGATGCC 
GACAAGGAGC GCCTGGCCAT CCTGGGCATC CACAATGAGG TGTCCAAGAT TGTCCAGACC 
TACCACGTCA ATATGGCGGG CACCAACCCC TACACAACCA TCACGCCTCA GGAGATCAAT 
GGCAAATGGG ACCACGTGCG GCAGCTGGTG CCTCGGAGGG ACCAAGCTCT GACGGAGGAG 
CATGCCCGAC AGCAGCACAA TGAGAGGCTA CGCAAGCAGT TTGGAGCCCA GGCCAATGTC 
ATCGGGCCCT GGATCCAGAC CAAGATGGAG GAGATCGGGA GGATCTCCAT TGAGATGCAT 
GGGACCCTGG AGGACCAGCT CAGCCACCTG CGGCAGTATG AGAAGAGCAT CGTCAACTAC 
AAGCCAAAGA TTGATCAGCT GGAGGGCGAC CACCAGCTCA TCCAGGAGGC GCTCATCTTC 
GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATCG CCAGGACCAT CAATGAGGTA GAGAACCAGA TCCTGACCCG GGATGCCAAG 
GGCATCAGCC AGGAGCAGAT GAATGAGTTC CGGGCCTCCT TCAACCACTT TGACCGGAAG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TGATCTCCAT GGGTTACAAC 
ATGGGAGAAG CAGAATTTGC CCGCATCATG AGCATTGTGG ACCCCAACCG CCTGGGGGTA 
GTGACATTCC AGGCCTTCAT TGACTTCATG TCCCGCGAGA CAGCCGACAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCTGGGGACA AGAACTACAT TACCATGGAC 
GAGCTGCGCC GCGAGCTGCC ACCCGACCAG GCTGAGTACT GCATCGCGCG GATGGCCCCC 
TACACCGGCC CCGACTCCGT GCCAGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877217.1
CDS	485..3085
Translation	MGLRYVLPLRPPGKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021728.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021728.1

ORF Insert Sequence:

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GGCAAAGAGG CCATGCTGCG ACAGAAGGAC 
TATGAGACCG CCACCCTCTC GGAGATCAAG GCCCTGCTCA AGAAGCATGA GGCCTTCGAG 
AGTGACCTGG CTGCCCACCA GGACCGTGTG GAGCAGATTG CCGCCATCGC ACAGGAGCTC 
AATGAGCTGG ACTATTATGA CTCACCCAGT GTCAACGCCC GTTGCCAAAA GATCTGTGAC 
CAGTGGGACA ATCTGGGGGC CCTAACTCAG AAGCGAAGGG AAGCTCTGGA GCGGACCGAG 
AAACTGCTGG AGACCATTGA CCAGCTGTAC TTGGAGTATG CCAAGCGGGC TGCACCCTTC 
AACAACTGGA TGGAGGGGGC CATGGAGGAC CTGCAGGACA CCTTCATTGT GCACACCATT 
GAGGAGATCC AGGGACTGAC CACAGCCCAT GAGCAGTTCA AGGCCACCCT CCCTGATGCC 
GACAAGGAGC GCCTGGCCAT CCTGGGCATC CACAATGAGG TGTCCAAGAT TGTCCAGACC 
TACCACGTCA ATATGGCGGG CACCAACCCC TACACAACCA TCACGCCTCA GGAGATCAAT 
GGCAAATGGG ACCACGTGCG GCAGCTGGTG CCTCGGAGGG ACCAAGCTCT GACGGAGGAG 
CATGCCCGAC AGCAGCACAA TGAGAGGCTA CGCAAGCAGT TTGGAGCCCA GGCCAATGTC 
ATCGGGCCCT GGATCCAGAC CAAGATGGAG GAGATCGGGA GGATCTCCAT TGAGATGCAT 
GGGACCCTGG AGGACCAGCT CAGCCACCTG CGGCAGTATG AGAAGAGCAT CGTCAACTAC 
AAGCCAAAGA TTGATCAGCT GGAGGGCGAC CACCAGCTCA TCCAGGAGGC GCTCATCTTC 
GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATCG CCAGGACCAT CAATGAGGTA GAGAACCAGA TCCTGACCCG GGATGCCAAG 
GGCATCAGCC AGGAGCAGAT GAATGAGTTC CGGGCCTCCT TCAACCACTT TGACCGGAAG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TGATCTCCAT GGGTTACAAC 
ATGGGAGAAG CAGAATTTGC CCGCATCATG AGCATTGTGG ACCCCAACCG CCTGGGGGTA 
GTGACATTCC AGGCCTTCAT TGACTTCATG TCCCGCGAGA CAGCCGACAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCTGGGGACA AGAACTACAT TACCATGGAC 
GAGCTGCGCC GCGAGCTGCC ACCCGACCAG GCTGAGTACT GCATCGCGCG GATGGCCCCC 
TACACCGGCC CCGACTCCGT GCCAGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021720.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877209.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu85139
Clone ID Related Accession (Same CDS sequence)	XM_017021720.1
Accession Version	XM_017021720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GCCATGTGGC CCTCTCTCAA CCCAGGCAAA GAGGCCATGC TGCGACAGAA GGACTATGAG 
ACCGCCACCC TCTCGGAGAT CAAGGCCCTG CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC 
CTGGCTGCCC ACCAGGACCG TGTGGAGCAG ATTGCCGCCA TCGCACAGGA GCTCAATGAG 
CTGGACTATT ATGACTCACC CAGTGTCAAC GCCCGTTGCC AAAAGATCTG TGACCAGTGG 
GACAATCTGG GGGCCCTAAC TCAGAAGCGA AGGGAAGCTC TGGAGCGGAC CGAGAAACTG 
CTGGAGACCA TTGACCAGCT GTACTTGGAG TATGCCAAGC GGGCTGCACC CTTCAACAAC 
TGGATGGAGG GGGCCATGGA GGACCTGCAG GACACCTTCA TTGTGCACAC CATTGAGGAG 
ATCCAGGGAC TGACCACAGC CCATGAGCAG TTCAAGGCCA CCCTCCCTGA TGCCGACAAG 
GAGCGCCTGG CCATCCTGGG CATCCACAAT GAGGTGTCCA AGATTGTCCA GACCTACCAC 
GTCAATATGG CGGGCACCAA CCCCTACACA ACCATCACGC CTCAGGAGAT CAATGGCAAA 
TGGGACCACG TGCGGCAGCT GGTGCCTCGG AGGGACCAAG CTCTGACGGA GGAGCATGCC 
CGACAGCAGC ACAATGAGAG GCTACGCAAG CAGTTTGGAG CCCAGGCCAA TGTCATCGGG 
CCCTGGATCC AGACCAAGAT GGAGGAGATC GGGAGGATCT CCATTGAGAT GCATGGGACC 
CTGGAGGACC AGCTCAGCCA CCTGCGGCAG TATGAGAAGA GCATCGTCAA CTACAAGCCA 
AAGATTGATC AGCTGGAGGG CGACCACCAG CTCATCCAGG AGGCGCTCAT CTTCGACAAC 
AAGCACACCA ACTACACCAT GGAGCACATC CGTGTGGGCT GGGAGCAGCT GCTCACCACC 
ATCGCCAGGA CCATCAATGA GGTAGAGAAC CAGATCCTGA CCCGGGATGC CAAGGGCATC 
AGCCAGGAGC AGATGAATGA GTTCCGGGCC TCCTTCAACC ACTTTGACCG GGATCACTCC 
GGCACACTGG GTCCCGAGGA GTTCAAAGCC TGCCTCATCA GCTTGGGTTA TGATATTGGC 
AACGACCCCC AGGTACTCGC CTCCTGCATG GAGCACGCTC AGGGGTGGCG TCTGGGGCAA 
GAAATAATGC GTTTTTCCTT TTTCTCTTCT CTCTGTCTGG ATCTCCTGTC CCCCTCCTGT 
GGGCCATGGG GCTCGTCCTC TCCTGCTGTC TCTGTCTGCC CCATCACTGG TCCACCCTCT 
GCCCTCTGCT GTCTTTCATG GCAACCTACT GGGTGCCCGC TGTCTTCCGT CCTCGCTGCT 
CTTCATCCCA TGGGTGGATT TTTGCATCTC CCTGCTCTGT CTTCCTCCTG TCTTTGGACA 
TTTCCACCGA TGTGTGTTCG CATCTTCTCC TATGTTCCTT TACCTATCCT GACCCCCAAA 
ACCATAAATC TCATCCCCGT TCTGGCCATC TGTTCCTGTC TTCCTGGCCC CGGGCCGGCC 
CTTCCTCTTC CTGCCTTCCC GACCCTCCTT GTGTCTTGGT ACCACTGCCC CCCACAGAAG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TGATCTCCAT GGGTTACAAC 
ATGGGAGAAG CAGAATTTGC CCGCATCATG AGCATTGTGG ACCCCAACCG CCTGGGGGTA 
GTGACATTCC AGGCCTTCAT TGACTTCATG TCCCGCGAGA CAGCCGACAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCTGGGGACA AGAACTACAT TACCATGGAC 
GAGCTGCGCC GCGAGCTGCC ACCCGACCAG GCTGAGTACT GCATCGCGCG GATGGCCCCC 
TACACCGGCC CCGACTCCGT GCCAGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877209.1
CDS	16..3336
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDAMWPSLNPGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021720.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021720.1

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GCCATGTGGC CCTCTCTCAA CCCAGGCAAA GAGGCCATGC TGCGACAGAA GGACTATGAG 
ACCGCCACCC TCTCGGAGAT CAAGGCCCTG CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC 
CTGGCTGCCC ACCAGGACCG TGTGGAGCAG ATTGCCGCCA TCGCACAGGA GCTCAATGAG 
CTGGACTATT ATGACTCACC CAGTGTCAAC GCCCGTTGCC AAAAGATCTG TGACCAGTGG 
GACAATCTGG GGGCCCTAAC TCAGAAGCGA AGGGAAGCTC TGGAGCGGAC CGAGAAACTG 
CTGGAGACCA TTGACCAGCT GTACTTGGAG TATGCCAAGC GGGCTGCACC CTTCAACAAC 
TGGATGGAGG GGGCCATGGA GGACCTGCAG GACACCTTCA TTGTGCACAC CATTGAGGAG 
ATCCAGGGAC TGACCACAGC CCATGAGCAG TTCAAGGCCA CCCTCCCTGA TGCCGACAAG 
GAGCGCCTGG CCATCCTGGG CATCCACAAT GAGGTGTCCA AGATTGTCCA GACCTACCAC 
GTCAATATGG CGGGCACCAA CCCCTACACA ACCATCACGC CTCAGGAGAT CAATGGCAAA 
TGGGACCACG TGCGGCAGCT GGTGCCTCGG AGGGACCAAG CTCTGACGGA GGAGCATGCC 
CGACAGCAGC ACAATGAGAG GCTACGCAAG CAGTTTGGAG CCCAGGCCAA TGTCATCGGG 
CCCTGGATCC AGACCAAGAT GGAGGAGATC GGGAGGATCT CCATTGAGAT GCATGGGACC 
CTGGAGGACC AGCTCAGCCA CCTGCGGCAG TATGAGAAGA GCATCGTCAA CTACAAGCCA 
AAGATTGATC AGCTGGAGGG CGACCACCAG CTCATCCAGG AGGCGCTCAT CTTCGACAAC 
AAGCACACCA ACTACACCAT GGAGCACATC CGTGTGGGCT GGGAGCAGCT GCTCACCACC 
ATCGCCAGGA CCATCAATGA GGTAGAGAAC CAGATCCTGA CCCGGGATGC CAAGGGCATC 
AGCCAGGAGC AGATGAATGA GTTCCGGGCC TCCTTCAACC ACTTTGACCG GGATCACTCC 
GGCACACTGG GTCCCGAGGA GTTCAAAGCC TGCCTCATCA GCTTGGGTTA TGATATTGGC 
AACGACCCCC AGGTACTCGC CTCCTGCATG GAGCACGCTC AGGGGTGGCG TCTGGGGCAA 
GAAATAATGC GTTTTTCCTT TTTCTCTTCT CTCTGTCTGG ATCTCCTGTC CCCCTCCTGT 
GGGCCATGGG GCTCGTCCTC TCCTGCTGTC TCTGTCTGCC CCATCACTGG TCCACCCTCT 
GCCCTCTGCT GTCTTTCATG GCAACCTACT GGGTGCCCGC TGTCTTCCGT CCTCGCTGCT 
CTTCATCCCA TGGGTGGATT TTTGCATCTC CCTGCTCTGT CTTCCTCCTG TCTTTGGACA 
TTTCCACCGA TGTGTGTTCG CATCTTCTCC TATGTTCCTT TACCTATCCT GACCCCCAAA 
ACCATAAATC TCATCCCCGT TCTGGCCATC TGTTCCTGTC TTCCTGGCCC CGGGCCGGCC 
CTTCCTCTTC CTGCCTTCCC GACCCTCCTT GTGTCTTGGT ACCACTGCCC CCCACAGAAG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TGATCTCCAT GGGTTACAAC 
ATGGGAGAAG CAGAATTTGC CCGCATCATG AGCATTGTGG ACCCCAACCG CCTGGGGGTA 
GTGACATTCC AGGCCTTCAT TGACTTCATG TCCCGCGAGA CAGCCGACAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCTGGGGACA AGAACTACAT TACCATGGAC 
GAGCTGCGCC GCGAGCTGCC ACCCGACCAG GCTGAGTACT GCATCGCGCG GATGGCCCCC 
TACACCGGCC CCGACTCCGT GCCAGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021721.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877210.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu85140
Clone ID Related Accession (Same CDS sequence)	XM_017021721.2 , XM_017021721.1
Accession Version	XM_017021721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGGAT CACTCCGGCA CACTGGGTCC CGAGGAGTTC 
AAAGCCTGCC TCATCAGCTT GGGTTATGAT ATTGGCAACG ACCCCCAGGT ACTCGCCTCC 
TGCATGGAGC ACGCTCAGGG GTGGCGTCTG GGGCAAGAAA TAATGCGTTT TTCCTTTTTC 
TCTTCTCTCT GTCTGGATCT CCTGTCCCCC TCCTGTGGGC CATGGGGCTC GTCCTCTCCT 
GCTGTCTCTG TCTGCCCCAT CACTGGTCCA CCCTCTGCCC TCTGCTGTCT TTCATGGCAA 
CCTACTGGGT GCCCGCTGTC TTCCGTCCTC GCTGCTCTTC ATCCCATGGG TGGATTTTTG 
CATCTCCCTG CTCTGTCTTC CTCCTGTCTT TGGACATTTC CACCGATGTG TGTTCGCATC 
TTCTCCTATG TTCCTTTACC TATCCTGACC CCCAAAACCA TAAATCTCAT CCCCGTTCTG 
GCCATCTGTT CCTGTCTTCC TGGCCCCGGG CCGGCCCTTC CTCTTCCTGC CTTCCCGACC 
CTCCTTGTGT CTTGGTACCA CTGCCCCCCA CAGAAGAAGA CAGGCATGAT GGACACGGAT 
GATTTCCGCG CCTGCCTGAT CTCCATGGGT TACAACATGG GAGAAGCAGA ATTTGCCCGC 
ATCATGAGCA TTGTGGACCC CAACCGCCTG GGGGTAGTGA CATTCCAGGC CTTCATTGAC 
TTCATGTCCC GCGAGACAGC CGACACAGAT ACAGCAGACC AAGTCATGGC TTCCTTCAAG 
ATCCTGGCTG GGGACAAGAA CTACATTACC ATGGACGAGC TGCGCCGCGA GCTGCCACCC 
GACCAGGCTG AGTACTGCAT CGCGCGGATG GCCCCCTACA CCGGCCCCGA CTCCGTGCCA 
GGTGCTCTGG ACTACATGTC CTTCTCCACG GCGCTGTACG GCGAGAGTGA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877210.1
CDS	16..3312
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021721.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021721.2

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGGAT CACTCCGGCA CACTGGGTCC CGAGGAGTTC 
AAAGCCTGCC TCATCAGCTT GGGTTATGAT ATTGGCAACG ACCCCCAGGT ACTCGCCTCC 
TGCATGGAGC ACGCTCAGGG GTGGCGTCTG GGGCAAGAAA TAATGCGTTT TTCCTTTTTC 
TCTTCTCTCT GTCTGGATCT CCTGTCCCCC TCCTGTGGGC CATGGGGCTC GTCCTCTCCT 
GCTGTCTCTG TCTGCCCCAT CACTGGTCCA CCCTCTGCCC TCTGCTGTCT TTCATGGCAA 
CCTACTGGGT GCCCGCTGTC TTCCGTCCTC GCTGCTCTTC ATCCCATGGG TGGATTTTTG 
CATCTCCCTG CTCTGTCTTC CTCCTGTCTT TGGACATTTC CACCGATGTG TGTTCGCATC 
TTCTCCTATG TTCCTTTACC TATCCTGACC CCCAAAACCA TAAATCTCAT CCCCGTTCTG 
GCCATCTGTT CCTGTCTTCC TGGCCCCGGG CCGGCCCTTC CTCTTCCTGC CTTCCCGACC 
CTCCTTGTGT CTTGGTACCA CTGCCCCCCA CAGAAGAAGA CAGGCATGAT GGACACGGAT 
GATTTCCGCG CCTGCCTGAT CTCCATGGGT TACAACATGG GAGAAGCAGA ATTTGCCCGC 
ATCATGAGCA TTGTGGACCC CAACCGCCTG GGGGTAGTGA CATTCCAGGC CTTCATTGAC 
TTCATGTCCC GCGAGACAGC CGACACAGAT ACAGCAGACC AAGTCATGGC TTCCTTCAAG 
ATCCTGGCTG GGGACAAGAA CTACATTACC ATGGACGAGC TGCGCCGCGA GCTGCCACCC 
GACCAGGCTG AGTACTGCAT CGCGCGGATG GCCCCCTACA CCGGCCCCGA CTCCGTGCCA 
GGTGCTCTGG ACTACATGTC CTTCTCCACG GCGCTGTACG GCGAGAGTGA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021721.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877210.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X2, mRNA.

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CloneID	OHu85140
Clone ID Related Accession (Same CDS sequence)	XM_017021721.2 , XM_017021721.1
Accession Version	XM_017021721.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGGAT CACTCCGGCA CACTGGGTCC CGAGGAGTTC 
AAAGCCTGCC TCATCAGCTT GGGTTATGAT ATTGGCAACG ACCCCCAGGT ACTCGCCTCC 
TGCATGGAGC ACGCTCAGGG GTGGCGTCTG GGGCAAGAAA TAATGCGTTT TTCCTTTTTC 
TCTTCTCTCT GTCTGGATCT CCTGTCCCCC TCCTGTGGGC CATGGGGCTC GTCCTCTCCT 
GCTGTCTCTG TCTGCCCCAT CACTGGTCCA CCCTCTGCCC TCTGCTGTCT TTCATGGCAA 
CCTACTGGGT GCCCGCTGTC TTCCGTCCTC GCTGCTCTTC ATCCCATGGG TGGATTTTTG 
CATCTCCCTG CTCTGTCTTC CTCCTGTCTT TGGACATTTC CACCGATGTG TGTTCGCATC 
TTCTCCTATG TTCCTTTACC TATCCTGACC CCCAAAACCA TAAATCTCAT CCCCGTTCTG 
GCCATCTGTT CCTGTCTTCC TGGCCCCGGG CCGGCCCTTC CTCTTCCTGC CTTCCCGACC 
CTCCTTGTGT CTTGGTACCA CTGCCCCCCA CAGAAGAAGA CAGGCATGAT GGACACGGAT 
GATTTCCGCG CCTGCCTGAT CTCCATGGGT TACAACATGG GAGAAGCAGA ATTTGCCCGC 
ATCATGAGCA TTGTGGACCC CAACCGCCTG GGGGTAGTGA CATTCCAGGC CTTCATTGAC 
TTCATGTCCC GCGAGACAGC CGACACAGAT ACAGCAGACC AAGTCATGGC TTCCTTCAAG 
ATCCTGGCTG GGGACAAGAA CTACATTACC ATGGACGAGC TGCGCCGCGA GCTGCCACCC 
GACCAGGCTG AGTACTGCAT CGCGCGGATG GCCCCCTACA CCGGCCCCGA CTCCGTGCCA 
GGTGCTCTGG ACTACATGTC CTTCTCCACG GCGCTGTACG GCGAGAGTGA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877210.1
CDS	16..3312
Translation	MVSNGLQVRVGVRDGARNPCRKADNCHQGIGKGKRKEEAYKSENPKKLKWLGEKTLKRPWSTSETFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021721.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021721.1

ORF Insert Sequence:

ATGGTATCAA ATGGACTGCA AGTGAGGGTG GGGGTGAGAG ACGGGGCCAG GAATCCATGC 
AGGAAAGCTG ATAACTGTCA CCAGGGAATT GGCAAGGGAA AAAGGAAGGA GGAGGCATAC 
AAGAGCGAGA ATCCAAAAAA GTTGAAATGG TTAGGGGAGA AAACTCTCAA AAGACCCTGG 
AGTACATCAG AGACATTCAC GGCATGGTGT AACTCCCACC TCCGGAAGGC GGGGACACAG 
ATCGAGAACA TCGAAGAGGA CTTCCGGGAT GGCCTGAAGC TCATGCTGCT GCTGGAGGTC 
ATCTCAGGTG AACGCTTGGC CAAGCCAGAG CGAGGCAAGA TGAGAGTGCA CAAGATCTCC 
AACGTCAACA AGGCCCTGGA TTTCATAGCC AGCAAAGGCG TCAAACTGGT GTCCATCGGA 
GCCGAAGAAA TCGTGGATGG GAATGTGAAG ATGACCCTGG GCATGATCTG GACCATCATC 
CTGCGCTTTG CCATCCAGGA CATCTCCGTG GAAGAGACTT CAGCCAAGGA AGGGCTGCTC 
CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC AAAAATGTCA ACATCCAGAA CTTCCACATA 
AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT TTGATCCACC GACACCGGCC CGAGCTGATT 
GACTACGGGA AGCTGCGGAA GGATGATCCA CTCACAAATC TGAATACGGC TTTTGACGTG 
GCAGAGAAGT ACCTGGACAT CCCCAAGATG CTGGATGCCG AAGACATCGT TGGAACTGCC 
CGACCGGATG AGAAAGCCAT CATGACTTAC GTGTCTAGCT TCTACCACGC CTTCTCTGGA 
GCCCAGAAGG CGGAGACAGC AGCCAATCGC ATCTGCAAGG TGTTGGCCGT CAACCAGGAG 
AACGAGCAGC TTATGGAAGA CTACGAGAAG CTGGCCAGTG ATCTGTTGGA GTGGATCCGC 
CGCACAATCC CGTGGCTGGA GAACCGGGTG CCCGAGAACA CCATGCATGC CATGCAACAG 
AAGCTGGAGG ACTTCCGGGA CTACCGGCGC CTGCACAAGC CGCCCAAGGT GCAGGAGAAG 
TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGC TGCGGCTCAG CAACCGGCCT 
GCCTTCATGC CCTCTGAGGG CAGGATGGTC TCGGACATCA ACAATGCCTG GGGCTGCCTG 
GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG TTGCTGAATG AGATCCGGAG GCTGGAGCGA 
CTGGACCACC TGGCAGAGAA GTTCCGGCAG AAGGCCTCCA TCCACGAGGC CTGGACTGAC 
GGCAAAGAGG CCATGCTGCG ACAGAAGGAC TATGAGACCG CCACCCTCTC GGAGATCAAG 
GCCCTGCTCA AGAAGCATGA GGCCTTCGAG AGTGACCTGG CTGCCCACCA GGACCGTGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTATTATGA CTCACCCAGT 
GTCAACGCCC GTTGCCAAAA GATCTGTGAC CAGTGGGACA ATCTGGGGGC CCTAACTCAG 
AAGCGAAGGG AAGCTCTGGA GCGGACCGAG AAACTGCTGG AGACCATTGA CCAGCTGTAC 
TTGGAGTATG CCAAGCGGGC TGCACCCTTC AACAACTGGA TGGAGGGGGC CATGGAGGAC 
CTGCAGGACA CCTTCATTGT GCACACCATT GAGGAGATCC AGGGACTGAC CACAGCCCAT 
GAGCAGTTCA AGGCCACCCT CCCTGATGCC GACAAGGAGC GCCTGGCCAT CCTGGGCATC 
CACAATGAGG TGTCCAAGAT TGTCCAGACC TACCACGTCA ATATGGCGGG CACCAACCCC 
TACACAACCA TCACGCCTCA GGAGATCAAT GGCAAATGGG ACCACGTGCG GCAGCTGGTG 
CCTCGGAGGG ACCAAGCTCT GACGGAGGAG CATGCCCGAC AGCAGCACAA TGAGAGGCTA 
CGCAAGCAGT TTGGAGCCCA GGCCAATGTC ATCGGGCCCT GGATCCAGAC CAAGATGGAG 
GAGATCGGGA GGATCTCCAT TGAGATGCAT GGGACCCTGG AGGACCAGCT CAGCCACCTG 
CGGCAGTATG AGAAGAGCAT CGTCAACTAC AAGCCAAAGA TTGATCAGCT GGAGGGCGAC 
CACCAGCTCA TCCAGGAGGC GCTCATCTTC GACAACAAGC ACACCAACTA CACCATGGAG 
CACATCCGTG TGGGCTGGGA GCAGCTGCTC ACCACCATCG CCAGGACCAT CAATGAGGTA 
GAGAACCAGA TCCTGACCCG GGATGCCAAG GGCATCAGCC AGGAGCAGAT GAATGAGTTC 
CGGGCCTCCT TCAACCACTT TGACCGGGAT CACTCCGGCA CACTGGGTCC CGAGGAGTTC 
AAAGCCTGCC TCATCAGCTT GGGTTATGAT ATTGGCAACG ACCCCCAGGT ACTCGCCTCC 
TGCATGGAGC ACGCTCAGGG GTGGCGTCTG GGGCAAGAAA TAATGCGTTT TTCCTTTTTC 
TCTTCTCTCT GTCTGGATCT CCTGTCCCCC TCCTGTGGGC CATGGGGCTC GTCCTCTCCT 
GCTGTCTCTG TCTGCCCCAT CACTGGTCCA CCCTCTGCCC TCTGCTGTCT TTCATGGCAA 
CCTACTGGGT GCCCGCTGTC TTCCGTCCTC GCTGCTCTTC ATCCCATGGG TGGATTTTTG 
CATCTCCCTG CTCTGTCTTC CTCCTGTCTT TGGACATTTC CACCGATGTG TGTTCGCATC 
TTCTCCTATG TTCCTTTACC TATCCTGACC CCCAAAACCA TAAATCTCAT CCCCGTTCTG 
GCCATCTGTT CCTGTCTTCC TGGCCCCGGG CCGGCCCTTC CTCTTCCTGC CTTCCCGACC 
CTCCTTGTGT CTTGGTACCA CTGCCCCCCA CAGAAGAAGA CAGGCATGAT GGACACGGAT 
GATTTCCGCG CCTGCCTGAT CTCCATGGGT TACAACATGG GAGAAGCAGA ATTTGCCCGC 
ATCATGAGCA TTGTGGACCC CAACCGCCTG GGGGTAGTGA CATTCCAGGC CTTCATTGAC 
TTCATGTCCC GCGAGACAGC CGACACAGAT ACAGCAGACC AAGTCATGGC TTCCTTCAAG 
ATCCTGGCTG GGGACAAGAA CTACATTACC ATGGACGAGC TGCGCCGCGA GCTGCCACCC 
GACCAGGCTG AGTACTGCAT CGCGCGGATG GCCCCCTACA CCGGCCCCGA CTCCGTGCCA 
GGTGCTCTGG ACTACATGTC CTTCTCCACG GCGCTGTACG GCGAGAGTGA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021722.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877211.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu85141
Clone ID Related Accession (Same CDS sequence)	XM_017021722.1 , XM_017021722.2
Accession Version	XM_017021722.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGCCATGT GGCCCTCTCT CAACCCAGGC 
AAAGAGGCCA TGCTGCGACA GAAGGACTAT GAGACCGCCA CCCTCTCGGA GATCAAGGCC 
CTGCTCAAGA AGCATGAGGC CTTCGAGAGT GACCTGGCTG CCCACCAGGA CCGTGTGGAG 
CAGATTGCCG CCATCGCACA GGAGCTCAAT GAGCTGGACT ATTATGACTC ACCCAGTGTC 
AACGCCCGTT GCCAAAAGAT CTGTGACCAG TGGGACAATC TGGGGGCCCT AACTCAGAAG 
CGAAGGGAAG CTCTGGAGCG GACCGAGAAA CTGCTGGAGA CCATTGACCA GCTGTACTTG 
GAGTATGCCA AGCGGGCTGC ACCCTTCAAC AACTGGATGG AGGGGGCCAT GGAGGACCTG 
CAGGACACCT TCATTGTGCA CACCATTGAG GAGATCCAGG GACTGACCAC AGCCCATGAG 
CAGTTCAAGG CCACCCTCCC TGATGCCGAC AAGGAGCGCC TGGCCATCCT GGGCATCCAC 
AATGAGGTGT CCAAGATTGT CCAGACCTAC CACGTCAATA TGGCGGGCAC CAACCCCTAC 
ACAACCATCA CGCCTCAGGA GATCAATGGC AAATGGGACC ACGTGCGGCA GCTGGTGCCT 
CGGAGGGACC AAGCTCTGAC GGAGGAGCAT GCCCGACAGC AGCACAATGA GAGGCTACGC 
AAGCAGTTTG GAGCCCAGGC CAATGTCATC GGGCCCTGGA TCCAGACCAA GATGGAGGAG 
ATCGGGAGGA TCTCCATTGA GATGCATGGG ACCCTGGAGG ACCAGCTCAG CCACCTGCGG 
CAGTATGAGA AGAGCATCGT CAACTACAAG CCAAAGATTG ATCAGCTGGA GGGCGACCAC 
CAGCTCATCC AGGAGGCGCT CATCTTCGAC AACAAGCACA CCAACTACAC CATGGAGCAC 
ATCCGTGTGG GCTGGGAGCA GCTGCTCACC ACCATCGCCA GGACCATCAA TGAGGTAGAG 
AACCAGATCC TGACCCGGGA TGCCAAGGGC ATCAGCCAGG AGCAGATGAA TGAGTTCCGG 
GCCTCCTTCA ACCACTTTGA CCGGGATCAC TCCGGCACAC TGGGTCCCGA GGAGTTCAAA 
GCCTGCCTCA TCAGCTTGGG TTATGATATT GGCAACGACC CCCAGGTACT CGCCTCCTGC 
ATGGAGCACG CTCAGGGGTG GCGTCTGGGG CAAGAAATAA TGCGTTTTTC CTTTTTCTCT 
TCTCTCTGTC TGGATCTCCT GTCCCCCTCC TGTGGGCCAT GGGGCTCGTC CTCTCCTGCT 
GTCTCTGTCT GCCCCATCAC TGGTCCACCC TCTGCCCTCT GCTGTCTTTC ATGGCAACCT 
ACTGGGTGCC CGCTGTCTTC CGTCCTCGCT GCTCTTCATC CCATGGGTGG ATTTTTGCAT 
CTCCCTGCTC TGTCTTCCTC CTGTCTTTGG ACATTTCCAC CGATGTGTGT TCGCATCTTC 
TCCTATGTTC CTTTACCTAT CCTGACCCCC AAAACCATAA ATCTCATCCC CGTTCTGGCC 
ATCTGTTCCT GTCTTCCTGG CCCCGGGCCG GCCCTTCCTC TTCCTGCCTT CCCGACCCTC 
CTTGTGTCTT GGTACCACTG CCCCCCACAG AAGAAGACAG GCATGATGGA CACGGATGAT 
TTCCGCGCCT GCCTGATCTC CATGGGTTAC AACATGGGAG AAGCAGAATT TGCCCGCATC 
ATGAGCATTG TGGACCCCAA CCGCCTGGGG GTAGTGACAT TCCAGGCCTT CATTGACTTC 
ATGTCCCGCG AGACAGCCGA CACAGATACA GCAGACCAAG TCATGGCTTC CTTCAAGATC 
CTGGCTGGGG ACAAGAACTA CATTACCATG GACGAGCTGC GCCGCGAGCT GCCACCCGAC 
CAGGCTGAGT ACTGCATCGC GCGGATGGCC CCCTACACCG GCCCCGACTC CGTGCCAGGT 
GCTCTGGACT ACATGTCCTT CTCCACGGCG CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877211.1
CDS	248..3481
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDAMWPSLNPGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021722.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021722.1

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGCCATGT GGCCCTCTCT CAACCCAGGC 
AAAGAGGCCA TGCTGCGACA GAAGGACTAT GAGACCGCCA CCCTCTCGGA GATCAAGGCC 
CTGCTCAAGA AGCATGAGGC CTTCGAGAGT GACCTGGCTG CCCACCAGGA CCGTGTGGAG 
CAGATTGCCG CCATCGCACA GGAGCTCAAT GAGCTGGACT ATTATGACTC ACCCAGTGTC 
AACGCCCGTT GCCAAAAGAT CTGTGACCAG TGGGACAATC TGGGGGCCCT AACTCAGAAG 
CGAAGGGAAG CTCTGGAGCG GACCGAGAAA CTGCTGGAGA CCATTGACCA GCTGTACTTG 
GAGTATGCCA AGCGGGCTGC ACCCTTCAAC AACTGGATGG AGGGGGCCAT GGAGGACCTG 
CAGGACACCT TCATTGTGCA CACCATTGAG GAGATCCAGG GACTGACCAC AGCCCATGAG 
CAGTTCAAGG CCACCCTCCC TGATGCCGAC AAGGAGCGCC TGGCCATCCT GGGCATCCAC 
AATGAGGTGT CCAAGATTGT CCAGACCTAC CACGTCAATA TGGCGGGCAC CAACCCCTAC 
ACAACCATCA CGCCTCAGGA GATCAATGGC AAATGGGACC ACGTGCGGCA GCTGGTGCCT 
CGGAGGGACC AAGCTCTGAC GGAGGAGCAT GCCCGACAGC AGCACAATGA GAGGCTACGC 
AAGCAGTTTG GAGCCCAGGC CAATGTCATC GGGCCCTGGA TCCAGACCAA GATGGAGGAG 
ATCGGGAGGA TCTCCATTGA GATGCATGGG ACCCTGGAGG ACCAGCTCAG CCACCTGCGG 
CAGTATGAGA AGAGCATCGT CAACTACAAG CCAAAGATTG ATCAGCTGGA GGGCGACCAC 
CAGCTCATCC AGGAGGCGCT CATCTTCGAC AACAAGCACA CCAACTACAC CATGGAGCAC 
ATCCGTGTGG GCTGGGAGCA GCTGCTCACC ACCATCGCCA GGACCATCAA TGAGGTAGAG 
AACCAGATCC TGACCCGGGA TGCCAAGGGC ATCAGCCAGG AGCAGATGAA TGAGTTCCGG 
GCCTCCTTCA ACCACTTTGA CCGGGATCAC TCCGGCACAC TGGGTCCCGA GGAGTTCAAA 
GCCTGCCTCA TCAGCTTGGG TTATGATATT GGCAACGACC CCCAGGTACT CGCCTCCTGC 
ATGGAGCACG CTCAGGGGTG GCGTCTGGGG CAAGAAATAA TGCGTTTTTC CTTTTTCTCT 
TCTCTCTGTC TGGATCTCCT GTCCCCCTCC TGTGGGCCAT GGGGCTCGTC CTCTCCTGCT 
GTCTCTGTCT GCCCCATCAC TGGTCCACCC TCTGCCCTCT GCTGTCTTTC ATGGCAACCT 
ACTGGGTGCC CGCTGTCTTC CGTCCTCGCT GCTCTTCATC CCATGGGTGG ATTTTTGCAT 
CTCCCTGCTC TGTCTTCCTC CTGTCTTTGG ACATTTCCAC CGATGTGTGT TCGCATCTTC 
TCCTATGTTC CTTTACCTAT CCTGACCCCC AAAACCATAA ATCTCATCCC CGTTCTGGCC 
ATCTGTTCCT GTCTTCCTGG CCCCGGGCCG GCCCTTCCTC TTCCTGCCTT CCCGACCCTC 
CTTGTGTCTT GGTACCACTG CCCCCCACAG AAGAAGACAG GCATGATGGA CACGGATGAT 
TTCCGCGCCT GCCTGATCTC CATGGGTTAC AACATGGGAG AAGCAGAATT TGCCCGCATC 
ATGAGCATTG TGGACCCCAA CCGCCTGGGG GTAGTGACAT TCCAGGCCTT CATTGACTTC 
ATGTCCCGCG AGACAGCCGA CACAGATACA GCAGACCAAG TCATGGCTTC CTTCAAGATC 
CTGGCTGGGG ACAAGAACTA CATTACCATG GACGAGCTGC GCCGCGAGCT GCCACCCGAC 
CAGGCTGAGT ACTGCATCGC GCGGATGGCC CCCTACACCG GCCCCGACTC CGTGCCAGGT 
GCTCTGGACT ACATGTCCTT CTCCACGGCG CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021722.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877211.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X3, mRNA.

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CloneID	OHu85141
Clone ID Related Accession (Same CDS sequence)	XM_017021722.1 , XM_017021722.2
Accession Version	XM_017021722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGCCATGT GGCCCTCTCT CAACCCAGGC 
AAAGAGGCCA TGCTGCGACA GAAGGACTAT GAGACCGCCA CCCTCTCGGA GATCAAGGCC 
CTGCTCAAGA AGCATGAGGC CTTCGAGAGT GACCTGGCTG CCCACCAGGA CCGTGTGGAG 
CAGATTGCCG CCATCGCACA GGAGCTCAAT GAGCTGGACT ATTATGACTC ACCCAGTGTC 
AACGCCCGTT GCCAAAAGAT CTGTGACCAG TGGGACAATC TGGGGGCCCT AACTCAGAAG 
CGAAGGGAAG CTCTGGAGCG GACCGAGAAA CTGCTGGAGA CCATTGACCA GCTGTACTTG 
GAGTATGCCA AGCGGGCTGC ACCCTTCAAC AACTGGATGG AGGGGGCCAT GGAGGACCTG 
CAGGACACCT TCATTGTGCA CACCATTGAG GAGATCCAGG GACTGACCAC AGCCCATGAG 
CAGTTCAAGG CCACCCTCCC TGATGCCGAC AAGGAGCGCC TGGCCATCCT GGGCATCCAC 
AATGAGGTGT CCAAGATTGT CCAGACCTAC CACGTCAATA TGGCGGGCAC CAACCCCTAC 
ACAACCATCA CGCCTCAGGA GATCAATGGC AAATGGGACC ACGTGCGGCA GCTGGTGCCT 
CGGAGGGACC AAGCTCTGAC GGAGGAGCAT GCCCGACAGC AGCACAATGA GAGGCTACGC 
AAGCAGTTTG GAGCCCAGGC CAATGTCATC GGGCCCTGGA TCCAGACCAA GATGGAGGAG 
ATCGGGAGGA TCTCCATTGA GATGCATGGG ACCCTGGAGG ACCAGCTCAG CCACCTGCGG 
CAGTATGAGA AGAGCATCGT CAACTACAAG CCAAAGATTG ATCAGCTGGA GGGCGACCAC 
CAGCTCATCC AGGAGGCGCT CATCTTCGAC AACAAGCACA CCAACTACAC CATGGAGCAC 
ATCCGTGTGG GCTGGGAGCA GCTGCTCACC ACCATCGCCA GGACCATCAA TGAGGTAGAG 
AACCAGATCC TGACCCGGGA TGCCAAGGGC ATCAGCCAGG AGCAGATGAA TGAGTTCCGG 
GCCTCCTTCA ACCACTTTGA CCGGGATCAC TCCGGCACAC TGGGTCCCGA GGAGTTCAAA 
GCCTGCCTCA TCAGCTTGGG TTATGATATT GGCAACGACC CCCAGGTACT CGCCTCCTGC 
ATGGAGCACG CTCAGGGGTG GCGTCTGGGG CAAGAAATAA TGCGTTTTTC CTTTTTCTCT 
TCTCTCTGTC TGGATCTCCT GTCCCCCTCC TGTGGGCCAT GGGGCTCGTC CTCTCCTGCT 
GTCTCTGTCT GCCCCATCAC TGGTCCACCC TCTGCCCTCT GCTGTCTTTC ATGGCAACCT 
ACTGGGTGCC CGCTGTCTTC CGTCCTCGCT GCTCTTCATC CCATGGGTGG ATTTTTGCAT 
CTCCCTGCTC TGTCTTCCTC CTGTCTTTGG ACATTTCCAC CGATGTGTGT TCGCATCTTC 
TCCTATGTTC CTTTACCTAT CCTGACCCCC AAAACCATAA ATCTCATCCC CGTTCTGGCC 
ATCTGTTCCT GTCTTCCTGG CCCCGGGCCG GCCCTTCCTC TTCCTGCCTT CCCGACCCTC 
CTTGTGTCTT GGTACCACTG CCCCCCACAG AAGAAGACAG GCATGATGGA CACGGATGAT 
TTCCGCGCCT GCCTGATCTC CATGGGTTAC AACATGGGAG AAGCAGAATT TGCCCGCATC 
ATGAGCATTG TGGACCCCAA CCGCCTGGGG GTAGTGACAT TCCAGGCCTT CATTGACTTC 
ATGTCCCGCG AGACAGCCGA CACAGATACA GCAGACCAAG TCATGGCTTC CTTCAAGATC 
CTGGCTGGGG ACAAGAACTA CATTACCATG GACGAGCTGC GCCGCGAGCT GCCACCCGAC 
CAGGCTGAGT ACTGCATCGC GCGGATGGCC CCCTACACCG GCCCCGACTC CGTGCCAGGT 
GCTCTGGACT ACATGTCCTT CTCCACGGCG CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877211.1
CDS	237..3470
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDAMWPSLNPGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021722.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021722.2

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGCCATGT GGCCCTCTCT CAACCCAGGC 
AAAGAGGCCA TGCTGCGACA GAAGGACTAT GAGACCGCCA CCCTCTCGGA GATCAAGGCC 
CTGCTCAAGA AGCATGAGGC CTTCGAGAGT GACCTGGCTG CCCACCAGGA CCGTGTGGAG 
CAGATTGCCG CCATCGCACA GGAGCTCAAT GAGCTGGACT ATTATGACTC ACCCAGTGTC 
AACGCCCGTT GCCAAAAGAT CTGTGACCAG TGGGACAATC TGGGGGCCCT AACTCAGAAG 
CGAAGGGAAG CTCTGGAGCG GACCGAGAAA CTGCTGGAGA CCATTGACCA GCTGTACTTG 
GAGTATGCCA AGCGGGCTGC ACCCTTCAAC AACTGGATGG AGGGGGCCAT GGAGGACCTG 
CAGGACACCT TCATTGTGCA CACCATTGAG GAGATCCAGG GACTGACCAC AGCCCATGAG 
CAGTTCAAGG CCACCCTCCC TGATGCCGAC AAGGAGCGCC TGGCCATCCT GGGCATCCAC 
AATGAGGTGT CCAAGATTGT CCAGACCTAC CACGTCAATA TGGCGGGCAC CAACCCCTAC 
ACAACCATCA CGCCTCAGGA GATCAATGGC AAATGGGACC ACGTGCGGCA GCTGGTGCCT 
CGGAGGGACC AAGCTCTGAC GGAGGAGCAT GCCCGACAGC AGCACAATGA GAGGCTACGC 
AAGCAGTTTG GAGCCCAGGC CAATGTCATC GGGCCCTGGA TCCAGACCAA GATGGAGGAG 
ATCGGGAGGA TCTCCATTGA GATGCATGGG ACCCTGGAGG ACCAGCTCAG CCACCTGCGG 
CAGTATGAGA AGAGCATCGT CAACTACAAG CCAAAGATTG ATCAGCTGGA GGGCGACCAC 
CAGCTCATCC AGGAGGCGCT CATCTTCGAC AACAAGCACA CCAACTACAC CATGGAGCAC 
ATCCGTGTGG GCTGGGAGCA GCTGCTCACC ACCATCGCCA GGACCATCAA TGAGGTAGAG 
AACCAGATCC TGACCCGGGA TGCCAAGGGC ATCAGCCAGG AGCAGATGAA TGAGTTCCGG 
GCCTCCTTCA ACCACTTTGA CCGGGATCAC TCCGGCACAC TGGGTCCCGA GGAGTTCAAA 
GCCTGCCTCA TCAGCTTGGG TTATGATATT GGCAACGACC CCCAGGTACT CGCCTCCTGC 
ATGGAGCACG CTCAGGGGTG GCGTCTGGGG CAAGAAATAA TGCGTTTTTC CTTTTTCTCT 
TCTCTCTGTC TGGATCTCCT GTCCCCCTCC TGTGGGCCAT GGGGCTCGTC CTCTCCTGCT 
GTCTCTGTCT GCCCCATCAC TGGTCCACCC TCTGCCCTCT GCTGTCTTTC ATGGCAACCT 
ACTGGGTGCC CGCTGTCTTC CGTCCTCGCT GCTCTTCATC CCATGGGTGG ATTTTTGCAT 
CTCCCTGCTC TGTCTTCCTC CTGTCTTTGG ACATTTCCAC CGATGTGTGT TCGCATCTTC 
TCCTATGTTC CTTTACCTAT CCTGACCCCC AAAACCATAA ATCTCATCCC CGTTCTGGCC 
ATCTGTTCCT GTCTTCCTGG CCCCGGGCCG GCCCTTCCTC TTCCTGCCTT CCCGACCCTC 
CTTGTGTCTT GGTACCACTG CCCCCCACAG AAGAAGACAG GCATGATGGA CACGGATGAT 
TTCCGCGCCT GCCTGATCTC CATGGGTTAC AACATGGGAG AAGCAGAATT TGCCCGCATC 
ATGAGCATTG TGGACCCCAA CCGCCTGGGG GTAGTGACAT TCCAGGCCTT CATTGACTTC 
ATGTCCCGCG AGACAGCCGA CACAGATACA GCAGACCAAG TCATGGCTTC CTTCAAGATC 
CTGGCTGGGG ACAAGAACTA CATTACCATG GACGAGCTGC GCCGCGAGCT GCCACCCGAC 
CAGGCTGAGT ACTGCATCGC GCGGATGGCC CCCTACACCG GCCCCGACTC CGTGCCAGGT 
GCTCTGGACT ACATGTCCTT CTCCACGGCG CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021723.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877212.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu85142
Clone ID Related Accession (Same CDS sequence)	XM_017021723.1 , XM_017021723.2
Accession Version	XM_017021723.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGTACTCGCC TCCTGCATGG AGCACGCTCA GGGGTGGCGT 
CTGGGGCAAG AAATAATGCG TTTTTCCTTT TTCTCTTCTC TCTGTCTGGA TCTCCTGTCC 
CCCTCCTGTG GGCCATGGGG CTCGTCCTCT CCTGCTGTCT CTGTCTGCCC CATCACTGGT 
CCACCCTCTG CCCTCTGCTG TCTTTCATGG CAACCTACTG GGTGCCCGCT GTCTTCCGTC 
CTCGCTGCTC TTCATCCCAT GGGTGGATTT TTGCATCTCC CTGCTCTGTC TTCCTCCTGT 
CTTTGGACAT TTCCACCGAT GTGTGTTCGC ATCTTCTCCT ATGTTCCTTT ACCTATCCTG 
ACCCCCAAAA CCATAAATCT CATCCCCGTT CTGGCCATCT GTTCCTGTCT TCCTGGCCCC 
GGGCCGGCCC TTCCTCTTCC TGCCTTCCCG ACCCTCCTTG TGTCTTGGTA CCACTGCCCC 
CCACAGAAGA AGACAGGCAT GATGGACACG GATGATTTCC GCGCCTGCCT GATCTCCATG 
GGTTACAACA TGGGAGAAGC AGAATTTGCC CGCATCATGA GCATTGTGGA CCCCAACCGC 
CTGGGGGTAG TGACATTCCA GGCCTTCATT GACTTCATGT CCCGCGAGAC AGCCGACACA 
GATACAGCAG ACCAAGTCAT GGCTTCCTTC AAGATCCTGG CTGGGGACAA GAACTACATT 
ACCATGGACG AGCTGCGCCG CGAGCTGCCA CCCGACCAGG CTGAGTACTG CATCGCGCGG 
ATGGCCCCCT ACACCGGCCC CGACTCCGTG CCAGGTGCTC TGGACTACAT GTCCTTCTCC 
ACGGCGCTGT ACGGCGAGAG TGACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877212.1
CDS	248..3457
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021723.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021723.1

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGTACTCGCC TCCTGCATGG AGCACGCTCA GGGGTGGCGT 
CTGGGGCAAG AAATAATGCG TTTTTCCTTT TTCTCTTCTC TCTGTCTGGA TCTCCTGTCC 
CCCTCCTGTG GGCCATGGGG CTCGTCCTCT CCTGCTGTCT CTGTCTGCCC CATCACTGGT 
CCACCCTCTG CCCTCTGCTG TCTTTCATGG CAACCTACTG GGTGCCCGCT GTCTTCCGTC 
CTCGCTGCTC TTCATCCCAT GGGTGGATTT TTGCATCTCC CTGCTCTGTC TTCCTCCTGT 
CTTTGGACAT TTCCACCGAT GTGTGTTCGC ATCTTCTCCT ATGTTCCTTT ACCTATCCTG 
ACCCCCAAAA CCATAAATCT CATCCCCGTT CTGGCCATCT GTTCCTGTCT TCCTGGCCCC 
GGGCCGGCCC TTCCTCTTCC TGCCTTCCCG ACCCTCCTTG TGTCTTGGTA CCACTGCCCC 
CCACAGAAGA AGACAGGCAT GATGGACACG GATGATTTCC GCGCCTGCCT GATCTCCATG 
GGTTACAACA TGGGAGAAGC AGAATTTGCC CGCATCATGA GCATTGTGGA CCCCAACCGC 
CTGGGGGTAG TGACATTCCA GGCCTTCATT GACTTCATGT CCCGCGAGAC AGCCGACACA 
GATACAGCAG ACCAAGTCAT GGCTTCCTTC AAGATCCTGG CTGGGGACAA GAACTACATT 
ACCATGGACG AGCTGCGCCG CGAGCTGCCA CCCGACCAGG CTGAGTACTG CATCGCGCGG 
ATGGCCCCCT ACACCGGCCC CGACTCCGTG CCAGGTGCTC TGGACTACAT GTCCTTCTCC 
ACGGCGCTGT ACGGCGAGAG TGACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021723.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877212.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu85142
Clone ID Related Accession (Same CDS sequence)	XM_017021723.1 , XM_017021723.2
Accession Version	XM_017021723.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGTACTCGCC TCCTGCATGG AGCACGCTCA GGGGTGGCGT 
CTGGGGCAAG AAATAATGCG TTTTTCCTTT TTCTCTTCTC TCTGTCTGGA TCTCCTGTCC 
CCCTCCTGTG GGCCATGGGG CTCGTCCTCT CCTGCTGTCT CTGTCTGCCC CATCACTGGT 
CCACCCTCTG CCCTCTGCTG TCTTTCATGG CAACCTACTG GGTGCCCGCT GTCTTCCGTC 
CTCGCTGCTC TTCATCCCAT GGGTGGATTT TTGCATCTCC CTGCTCTGTC TTCCTCCTGT 
CTTTGGACAT TTCCACCGAT GTGTGTTCGC ATCTTCTCCT ATGTTCCTTT ACCTATCCTG 
ACCCCCAAAA CCATAAATCT CATCCCCGTT CTGGCCATCT GTTCCTGTCT TCCTGGCCCC 
GGGCCGGCCC TTCCTCTTCC TGCCTTCCCG ACCCTCCTTG TGTCTTGGTA CCACTGCCCC 
CCACAGAAGA AGACAGGCAT GATGGACACG GATGATTTCC GCGCCTGCCT GATCTCCATG 
GGTTACAACA TGGGAGAAGC AGAATTTGCC CGCATCATGA GCATTGTGGA CCCCAACCGC 
CTGGGGGTAG TGACATTCCA GGCCTTCATT GACTTCATGT CCCGCGAGAC AGCCGACACA 
GATACAGCAG ACCAAGTCAT GGCTTCCTTC AAGATCCTGG CTGGGGACAA GAACTACATT 
ACCATGGACG AGCTGCGCCG CGAGCTGCCA CCCGACCAGG CTGAGTACTG CATCGCGCGG 
ATGGCCCCCT ACACCGGCCC CGACTCCGTG CCAGGTGCTC TGGACTACAT GTCCTTCTCC 
ACGGCGCTGT ACGGCGAGAG TGACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877212.1
CDS	237..3446
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021723.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021723.2

ORF Insert Sequence:

ATGGACCATT ATGATTCTCA GCAAACCAAC GATTACATGC AGCCAGAAGA GGACTGGGAC 
CGGGACCTGC TCCTGGACCC GGCCTGGGAG AAGCAGCAGA GAAAGACATT CACGGCATGG 
TGTAACTCCC ACCTCCGGAA GGCGGGGACA CAGATCGAGA ACATCGAAGA GGACTTCCGG 
GATGGCCTGA AGCTCATGCT GCTGCTGGAG GTCATCTCAG GTGAACGCTT GGCCAAGCCA 
GAGCGAGGCA AGATGAGAGT GCACAAGATC TCCAACGTCA ACAAGGCCCT GGATTTCATA 
GCCAGCAAAG GCGTCAAACT GGTGTCCATC GGAGCCGAAG AAATCGTGGA TGGGAATGTG 
AAGATGACCC TGGGCATGAT CTGGACCATC ATCCTGCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCCAA GGAAGGGCTG CTCCTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAAAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGATGGCCT CGGCTTCTGT 
GCTTTGATCC ACCGACACCG GCCCGAGCTG ATTGACTACG GGAAGCTGCG GAAGGATGAT 
CCACTCACAA ATCTGAATAC GGCTTTTGAC GTGGCAGAGA AGTACCTGGA CATCCCCAAG 
ATGCTGGATG CCGAAGACAT CGTTGGAACT GCCCGACCGG ATGAGAAAGC CATCATGACT 
TACGTGTCTA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCGGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGTTGGC CGTCAACCAG GAGAACGAGC AGCTTATGGA AGACTACGAG 
AAGCTGGCCA GTGATCTGTT GGAGTGGATC CGCCGCACAA TCCCGTGGCT GGAGAACCGG 
GTGCCCGAGA ACACCATGCA TGCCATGCAA CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGGCT CAGCAACCGG CCTGCCTTCA TGCCCTCTGA GGGCAGGATG 
GTCTCGGACA TCAACAATGC CTGGGGCTGC CTGGAGCAGG TGGAGAAGGG CTATGAGGAG 
TGGTTGCTGA ATGAGATCCG GAGGCTGGAG CGACTGGACC ACCTGGCAGA GAAGTTCCGG 
CAGAAGGCCT CCATCCACGA GGCCTGGACT GACGGCAAAG AGGCCATGCT GCGACAGAAG 
GACTATGAGA CCGCCACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA TGAGGCCTTC 
GAGAGTGACC TGGCTGCCCA CCAGGACCGT GTGGAGCAGA TTGCCGCCAT CGCACAGGAG 
CTCAATGAGC TGGACTATTA TGACTCACCC AGTGTCAACG CCCGTTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTGGG GGCCCTAACT CAGAAGCGAA GGGAAGCTCT GGAGCGGACC 
GAGAAACTGC TGGAGACCAT TGACCAGCTG TACTTGGAGT ATGCCAAGCG GGCTGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATTGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCTGAT 
GCCGACAAGG AGCGCCTGGC CATCCTGGGC ATCCACAATG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAATATGGC GGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCACGT GCGGCAGCTG GTGCCTCGGA GGGACCAAGC TCTGACGGAG 
GAGCATGCCC GACAGCAGCA CAATGAGAGG CTACGCAAGC AGTTTGGAGC CCAGGCCAAT 
GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATCG GGAGGATCTC CATTGAGATG 
CATGGGACCC TGGAGGACCA GCTCAGCCAC CTGCGGCAGT ATGAGAAGAG CATCGTCAAC 
TACAAGCCAA AGATTGATCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC 
TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTG 
CTCACCACCA TCGCCAGGAC CATCAATGAG GTAGAGAACC AGATCCTGAC CCGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACACTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGTACTCGCC TCCTGCATGG AGCACGCTCA GGGGTGGCGT 
CTGGGGCAAG AAATAATGCG TTTTTCCTTT TTCTCTTCTC TCTGTCTGGA TCTCCTGTCC 
CCCTCCTGTG GGCCATGGGG CTCGTCCTCT CCTGCTGTCT CTGTCTGCCC CATCACTGGT 
CCACCCTCTG CCCTCTGCTG TCTTTCATGG CAACCTACTG GGTGCCCGCT GTCTTCCGTC 
CTCGCTGCTC TTCATCCCAT GGGTGGATTT TTGCATCTCC CTGCTCTGTC TTCCTCCTGT 
CTTTGGACAT TTCCACCGAT GTGTGTTCGC ATCTTCTCCT ATGTTCCTTT ACCTATCCTG 
ACCCCCAAAA CCATAAATCT CATCCCCGTT CTGGCCATCT GTTCCTGTCT TCCTGGCCCC 
GGGCCGGCCC TTCCTCTTCC TGCCTTCCCG ACCCTCCTTG TGTCTTGGTA CCACTGCCCC 
CCACAGAAGA AGACAGGCAT GATGGACACG GATGATTTCC GCGCCTGCCT GATCTCCATG 
GGTTACAACA TGGGAGAAGC AGAATTTGCC CGCATCATGA GCATTGTGGA CCCCAACCGC 
CTGGGGGTAG TGACATTCCA GGCCTTCATT GACTTCATGT CCCGCGAGAC AGCCGACACA 
GATACAGCAG ACCAAGTCAT GGCTTCCTTC AAGATCCTGG CTGGGGACAA GAACTACATT 
ACCATGGACG AGCTGCGCCG CGAGCTGCCA CCCGACCAGG CTGAGTACTG CATCGCGCGG 
ATGGCCCCCT ACACCGGCCC CGACTCCGTG CCAGGTGCTC TGGACTACAT GTCCTTCTCC 
ACGGCGCTGT ACGGCGAGAG TGACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021725.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877214.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu85143
Clone ID Related Accession (Same CDS sequence)	XM_017021725.1
Accession Version	XM_017021725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GCCATGTGGC CCTCTCTCAA CCCAGGCAAA 
GAGGCCATGC TGCGACAGAA GGACTATGAG ACCGCCACCC TCTCGGAGAT CAAGGCCCTG 
CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC CTGGCTGCCC ACCAGGACCG TGTGGAGCAG 
ATTGCCGCCA TCGCACAGGA GCTCAATGAG CTGGACTATT ATGACTCACC CAGTGTCAAC 
GCCCGTTGCC AAAAGATCTG TGACCAGTGG GACAATCTGG GGGCCCTAAC TCAGAAGCGA 
AGGGAAGCTC TGGAGCGGAC CGAGAAACTG CTGGAGACCA TTGACCAGCT GTACTTGGAG 
TATGCCAAGC GGGCTGCACC CTTCAACAAC TGGATGGAGG GGGCCATGGA GGACCTGCAG 
GACACCTTCA TTGTGCACAC CATTGAGGAG ATCCAGGGAC TGACCACAGC CCATGAGCAG 
TTCAAGGCCA CCCTCCCTGA TGCCGACAAG GAGCGCCTGG CCATCCTGGG CATCCACAAT 
GAGGTGTCCA AGATTGTCCA GACCTACCAC GTCAATATGG CGGGCACCAA CCCCTACACA 
ACCATCACGC CTCAGGAGAT CAATGGCAAA TGGGACCACG TGCGGCAGCT GGTGCCTCGG 
AGGGACCAAG CTCTGACGGA GGAGCATGCC CGACAGCAGC ACAATGAGAG GCTACGCAAG 
CAGTTTGGAG CCCAGGCCAA TGTCATCGGG CCCTGGATCC AGACCAAGAT GGAGGAGATC 
GGGAGGATCT CCATTGAGAT GCATGGGACC CTGGAGGACC AGCTCAGCCA CCTGCGGCAG 
TATGAGAAGA GCATCGTCAA CTACAAGCCA AAGATTGATC AGCTGGAGGG CGACCACCAG 
CTCATCCAGG AGGCGCTCAT CTTCGACAAC AAGCACACCA ACTACACCAT GGAGCACATC 
CGTGTGGGCT GGGAGCAGCT GCTCACCACC ATCGCCAGGA CCATCAATGA GGTAGAGAAC 
CAGATCCTGA CCCGGGATGC CAAGGGCATC AGCCAGGAGC AGATGAATGA GTTCCGGGCC 
TCCTTCAACC ACTTTGACCG GGATCACTCC GGCACACTGG GTCCCGAGGA GTTCAAAGCC 
TGCCTCATCA GCTTGGGTTA TGATATTGGC AACGACCCCC AGGTACTCGC CTCCTGCATG 
GAGCACGCTC AGGGGTGGCG TCTGGGGCAA GAAATAATGC GTTTTTCCTT TTTCTCTTCT 
CTCTGTCTGG ATCTCCTGTC CCCCTCCTGT GGGCCATGGG GCTCGTCCTC TCCTGCTGTC 
TCTGTCTGCC CCATCACTGG TCCACCCTCT GCCCTCTGCT GTCTTTCATG GCAACCTACT 
GGGTGCCCGC TGTCTTCCGT CCTCGCTGCT CTTCATCCCA TGGGTGGATT TTTGCATCTC 
CCTGCTCTGT CTTCCTCCTG TCTTTGGACA TTTCCACCGA TGTGTGTTCG CATCTTCTCC 
TATGTTCCTT TACCTATCCT GACCCCCAAA ACCATAAATC TCATCCCCGT TCTGGCCATC 
TGTTCCTGTC TTCCTGGCCC CGGGCCGGCC CTTCCTCTTC CTGCCTTCCC GACCCTCCTT 
GTGTCTTGGT ACCACTGCCC CCCACAGAAG AAGACAGGCA TGATGGACAC GGATGATTTC 
CGCGCCTGCC TGATCTCCAT GGGTTACAAC ATGGGAGAAG CAGAATTTGC CCGCATCATG 
AGCATTGTGG ACCCCAACCG CCTGGGGGTA GTGACATTCC AGGCCTTCAT TGACTTCATG 
TCCCGCGAGA CAGCCGACAC AGATACAGCA GACCAAGTCA TGGCTTCCTT CAAGATCCTG 
GCTGGGGACA AGAACTACAT TACCATGGAC GAGCTGCGCC GCGAGCTGCC ACCCGACCAG 
GCTGAGTACT GCATCGCGCG GATGGCCCCC TACACCGGCC CCGACTCCGT GCCAGGTGCT 
CTGGACTACA TGTCCTTCTC CACGGCGCTG TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877214.1
CDS	485..3655
Translation	MGLRYVLPLRPPGKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDAMWPSLNPGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021725.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021725.1

ORF Insert Sequence:

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GCCATGTGGC CCTCTCTCAA CCCAGGCAAA 
GAGGCCATGC TGCGACAGAA GGACTATGAG ACCGCCACCC TCTCGGAGAT CAAGGCCCTG 
CTCAAGAAGC ATGAGGCCTT CGAGAGTGAC CTGGCTGCCC ACCAGGACCG TGTGGAGCAG 
ATTGCCGCCA TCGCACAGGA GCTCAATGAG CTGGACTATT ATGACTCACC CAGTGTCAAC 
GCCCGTTGCC AAAAGATCTG TGACCAGTGG GACAATCTGG GGGCCCTAAC TCAGAAGCGA 
AGGGAAGCTC TGGAGCGGAC CGAGAAACTG CTGGAGACCA TTGACCAGCT GTACTTGGAG 
TATGCCAAGC GGGCTGCACC CTTCAACAAC TGGATGGAGG GGGCCATGGA GGACCTGCAG 
GACACCTTCA TTGTGCACAC CATTGAGGAG ATCCAGGGAC TGACCACAGC CCATGAGCAG 
TTCAAGGCCA CCCTCCCTGA TGCCGACAAG GAGCGCCTGG CCATCCTGGG CATCCACAAT 
GAGGTGTCCA AGATTGTCCA GACCTACCAC GTCAATATGG CGGGCACCAA CCCCTACACA 
ACCATCACGC CTCAGGAGAT CAATGGCAAA TGGGACCACG TGCGGCAGCT GGTGCCTCGG 
AGGGACCAAG CTCTGACGGA GGAGCATGCC CGACAGCAGC ACAATGAGAG GCTACGCAAG 
CAGTTTGGAG CCCAGGCCAA TGTCATCGGG CCCTGGATCC AGACCAAGAT GGAGGAGATC 
GGGAGGATCT CCATTGAGAT GCATGGGACC CTGGAGGACC AGCTCAGCCA CCTGCGGCAG 
TATGAGAAGA GCATCGTCAA CTACAAGCCA AAGATTGATC AGCTGGAGGG CGACCACCAG 
CTCATCCAGG AGGCGCTCAT CTTCGACAAC AAGCACACCA ACTACACCAT GGAGCACATC 
CGTGTGGGCT GGGAGCAGCT GCTCACCACC ATCGCCAGGA CCATCAATGA GGTAGAGAAC 
CAGATCCTGA CCCGGGATGC CAAGGGCATC AGCCAGGAGC AGATGAATGA GTTCCGGGCC 
TCCTTCAACC ACTTTGACCG GGATCACTCC GGCACACTGG GTCCCGAGGA GTTCAAAGCC 
TGCCTCATCA GCTTGGGTTA TGATATTGGC AACGACCCCC AGGTACTCGC CTCCTGCATG 
GAGCACGCTC AGGGGTGGCG TCTGGGGCAA GAAATAATGC GTTTTTCCTT TTTCTCTTCT 
CTCTGTCTGG ATCTCCTGTC CCCCTCCTGT GGGCCATGGG GCTCGTCCTC TCCTGCTGTC 
TCTGTCTGCC CCATCACTGG TCCACCCTCT GCCCTCTGCT GTCTTTCATG GCAACCTACT 
GGGTGCCCGC TGTCTTCCGT CCTCGCTGCT CTTCATCCCA TGGGTGGATT TTTGCATCTC 
CCTGCTCTGT CTTCCTCCTG TCTTTGGACA TTTCCACCGA TGTGTGTTCG CATCTTCTCC 
TATGTTCCTT TACCTATCCT GACCCCCAAA ACCATAAATC TCATCCCCGT TCTGGCCATC 
TGTTCCTGTC TTCCTGGCCC CGGGCCGGCC CTTCCTCTTC CTGCCTTCCC GACCCTCCTT 
GTGTCTTGGT ACCACTGCCC CCCACAGAAG AAGACAGGCA TGATGGACAC GGATGATTTC 
CGCGCCTGCC TGATCTCCAT GGGTTACAAC ATGGGAGAAG CAGAATTTGC CCGCATCATG 
AGCATTGTGG ACCCCAACCG CCTGGGGGTA GTGACATTCC AGGCCTTCAT TGACTTCATG 
TCCCGCGAGA CAGCCGACAC AGATACAGCA GACCAAGTCA TGGCTTCCTT CAAGATCCTG 
GCTGGGGACA AGAACTACAT TACCATGGAC GAGCTGCGCC GCGAGCTGCC ACCCGACCAG 
GCTGAGTACT GCATCGCGCG GATGGCCCCC TACACCGGCC CCGACTCCGT GCCAGGTGCT 
CTGGACTACA TGTCCTTCTC CACGGCGCTG TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021726.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877215.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu85144
Clone ID Related Accession (Same CDS sequence)	XM_017021726.2 , XM_017021726.1
Accession Version	XM_017021726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GGCAAAGAGG CCATGCTGCG ACAGAAGGAC 
TATGAGACCG CCACCCTCTC GGAGATCAAG GCCCTGCTCA AGAAGCATGA GGCCTTCGAG 
AGTGACCTGG CTGCCCACCA GGACCGTGTG GAGCAGATTG CCGCCATCGC ACAGGAGCTC 
AATGAGCTGG ACTATTATGA CTCACCCAGT GTCAACGCCC GTTGCCAAAA GATCTGTGAC 
CAGTGGGACA ATCTGGGGGC CCTAACTCAG AAGCGAAGGG AAGCTCTGGA GCGGACCGAG 
AAACTGCTGG AGACCATTGA CCAGCTGTAC TTGGAGTATG CCAAGCGGGC TGCACCCTTC 
AACAACTGGA TGGAGGGGGC CATGGAGGAC CTGCAGGACA CCTTCATTGT GCACACCATT 
GAGGAGATCC AGGGACTGAC CACAGCCCAT GAGCAGTTCA AGGCCACCCT CCCTGATGCC 
GACAAGGAGC GCCTGGCCAT CCTGGGCATC CACAATGAGG TGTCCAAGAT TGTCCAGACC 
TACCACGTCA ATATGGCGGG CACCAACCCC TACACAACCA TCACGCCTCA GGAGATCAAT 
GGCAAATGGG ACCACGTGCG GCAGCTGGTG CCTCGGAGGG ACCAAGCTCT GACGGAGGAG 
CATGCCCGAC AGCAGCACAA TGAGAGGCTA CGCAAGCAGT TTGGAGCCCA GGCCAATGTC 
ATCGGGCCCT GGATCCAGAC CAAGATGGAG GAGATCGGGA GGATCTCCAT TGAGATGCAT 
GGGACCCTGG AGGACCAGCT CAGCCACCTG CGGCAGTATG AGAAGAGCAT CGTCAACTAC 
AAGCCAAAGA TTGATCAGCT GGAGGGCGAC CACCAGCTCA TCCAGGAGGC GCTCATCTTC 
GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATCG CCAGGACCAT CAATGAGGTA GAGAACCAGA TCCTGACCCG GGATGCCAAG 
GGCATCAGCC AGGAGCAGAT GAATGAGTTC CGGGCCTCCT TCAACCACTT TGACCGGGAT 
CACTCCGGCA CACTGGGTCC CGAGGAGTTC AAAGCCTGCC TCATCAGCTT GGGTTATGAT 
ATTGGCAACG ACCCCCAGGT ACTCGCCTCC TGCATGGAGC ACGCTCAGGG GTGGCGTCTG 
GGGCAAGAAA TAATGCGTTT TTCCTTTTTC TCTTCTCTCT GTCTGGATCT CCTGTCCCCC 
TCCTGTGGGC CATGGGGCTC GTCCTCTCCT GCTGTCTCTG TCTGCCCCAT CACTGGTCCA 
CCCTCTGCCC TCTGCTGTCT TTCATGGCAA CCTACTGGGT GCCCGCTGTC TTCCGTCCTC 
GCTGCTCTTC ATCCCATGGG TGGATTTTTG CATCTCCCTG CTCTGTCTTC CTCCTGTCTT 
TGGACATTTC CACCGATGTG TGTTCGCATC TTCTCCTATG TTCCTTTACC TATCCTGACC 
CCCAAAACCA TAAATCTCAT CCCCGTTCTG GCCATCTGTT CCTGTCTTCC TGGCCCCGGG 
CCGGCCCTTC CTCTTCCTGC CTTCCCGACC CTCCTTGTGT CTTGGTACCA CTGCCCCCCA 
CAGAAGAAGA CAGGCATGAT GGACACGGAT GATTTCCGCG CCTGCCTGAT CTCCATGGGT 
TACAACATGG GAGAAGCAGA ATTTGCCCGC ATCATGAGCA TTGTGGACCC CAACCGCCTG 
GGGGTAGTGA CATTCCAGGC CTTCATTGAC TTCATGTCCC GCGAGACAGC CGACACAGAT 
ACAGCAGACC AAGTCATGGC TTCCTTCAAG ATCCTGGCTG GGGACAAGAA CTACATTACC 
ATGGACGAGC TGCGCCGCGA GCTGCCACCC GACCAGGCTG AGTACTGCAT CGCGCGGATG 
GCCCCCTACA CCGGCCCCGA CTCCGTGCCA GGTGCTCTGG ACTACATGTC CTTCTCCACG 
GCGCTGTACG GCGAGAGTGA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877215.1
CDS	485..3631
Translation	MGLRYVLPLRPPGKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021726.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021726.2

ORF Insert Sequence:

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GGCAAAGAGG CCATGCTGCG ACAGAAGGAC 
TATGAGACCG CCACCCTCTC GGAGATCAAG GCCCTGCTCA AGAAGCATGA GGCCTTCGAG 
AGTGACCTGG CTGCCCACCA GGACCGTGTG GAGCAGATTG CCGCCATCGC ACAGGAGCTC 
AATGAGCTGG ACTATTATGA CTCACCCAGT GTCAACGCCC GTTGCCAAAA GATCTGTGAC 
CAGTGGGACA ATCTGGGGGC CCTAACTCAG AAGCGAAGGG AAGCTCTGGA GCGGACCGAG 
AAACTGCTGG AGACCATTGA CCAGCTGTAC TTGGAGTATG CCAAGCGGGC TGCACCCTTC 
AACAACTGGA TGGAGGGGGC CATGGAGGAC CTGCAGGACA CCTTCATTGT GCACACCATT 
GAGGAGATCC AGGGACTGAC CACAGCCCAT GAGCAGTTCA AGGCCACCCT CCCTGATGCC 
GACAAGGAGC GCCTGGCCAT CCTGGGCATC CACAATGAGG TGTCCAAGAT TGTCCAGACC 
TACCACGTCA ATATGGCGGG CACCAACCCC TACACAACCA TCACGCCTCA GGAGATCAAT 
GGCAAATGGG ACCACGTGCG GCAGCTGGTG CCTCGGAGGG ACCAAGCTCT GACGGAGGAG 
CATGCCCGAC AGCAGCACAA TGAGAGGCTA CGCAAGCAGT TTGGAGCCCA GGCCAATGTC 
ATCGGGCCCT GGATCCAGAC CAAGATGGAG GAGATCGGGA GGATCTCCAT TGAGATGCAT 
GGGACCCTGG AGGACCAGCT CAGCCACCTG CGGCAGTATG AGAAGAGCAT CGTCAACTAC 
AAGCCAAAGA TTGATCAGCT GGAGGGCGAC CACCAGCTCA TCCAGGAGGC GCTCATCTTC 
GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATCG CCAGGACCAT CAATGAGGTA GAGAACCAGA TCCTGACCCG GGATGCCAAG 
GGCATCAGCC AGGAGCAGAT GAATGAGTTC CGGGCCTCCT TCAACCACTT TGACCGGGAT 
CACTCCGGCA CACTGGGTCC CGAGGAGTTC AAAGCCTGCC TCATCAGCTT GGGTTATGAT 
ATTGGCAACG ACCCCCAGGT ACTCGCCTCC TGCATGGAGC ACGCTCAGGG GTGGCGTCTG 
GGGCAAGAAA TAATGCGTTT TTCCTTTTTC TCTTCTCTCT GTCTGGATCT CCTGTCCCCC 
TCCTGTGGGC CATGGGGCTC GTCCTCTCCT GCTGTCTCTG TCTGCCCCAT CACTGGTCCA 
CCCTCTGCCC TCTGCTGTCT TTCATGGCAA CCTACTGGGT GCCCGCTGTC TTCCGTCCTC 
GCTGCTCTTC ATCCCATGGG TGGATTTTTG CATCTCCCTG CTCTGTCTTC CTCCTGTCTT 
TGGACATTTC CACCGATGTG TGTTCGCATC TTCTCCTATG TTCCTTTACC TATCCTGACC 
CCCAAAACCA TAAATCTCAT CCCCGTTCTG GCCATCTGTT CCTGTCTTCC TGGCCCCGGG 
CCGGCCCTTC CTCTTCCTGC CTTCCCGACC CTCCTTGTGT CTTGGTACCA CTGCCCCCCA 
CAGAAGAAGA CAGGCATGAT GGACACGGAT GATTTCCGCG CCTGCCTGAT CTCCATGGGT 
TACAACATGG GAGAAGCAGA ATTTGCCCGC ATCATGAGCA TTGTGGACCC CAACCGCCTG 
GGGGTAGTGA CATTCCAGGC CTTCATTGAC TTCATGTCCC GCGAGACAGC CGACACAGAT 
ACAGCAGACC AAGTCATGGC TTCCTTCAAG ATCCTGGCTG GGGACAAGAA CTACATTACC 
ATGGACGAGC TGCGCCGCGA GCTGCCACCC GACCAGGCTG AGTACTGCAT CGCGCGGATG 
GCCCCCTACA CCGGCCCCGA CTCCGTGCCA GGTGCTCTGG ACTACATGTC CTTCTCCACG 
GCGCTGTACG GCGAGAGTGA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_017021726.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877215.1 Homo sapiens actinin alpha 1 (ACTN1), transcript variant X6, mRNA.

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CloneID	OHu85144
Clone ID Related Accession (Same CDS sequence)	XM_017021726.2 , XM_017021726.1
Accession Version	XM_017021726.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	alpha-actinin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GGCAAAGAGG CCATGCTGCG ACAGAAGGAC 
TATGAGACCG CCACCCTCTC GGAGATCAAG GCCCTGCTCA AGAAGCATGA GGCCTTCGAG 
AGTGACCTGG CTGCCCACCA GGACCGTGTG GAGCAGATTG CCGCCATCGC ACAGGAGCTC 
AATGAGCTGG ACTATTATGA CTCACCCAGT GTCAACGCCC GTTGCCAAAA GATCTGTGAC 
CAGTGGGACA ATCTGGGGGC CCTAACTCAG AAGCGAAGGG AAGCTCTGGA GCGGACCGAG 
AAACTGCTGG AGACCATTGA CCAGCTGTAC TTGGAGTATG CCAAGCGGGC TGCACCCTTC 
AACAACTGGA TGGAGGGGGC CATGGAGGAC CTGCAGGACA CCTTCATTGT GCACACCATT 
GAGGAGATCC AGGGACTGAC CACAGCCCAT GAGCAGTTCA AGGCCACCCT CCCTGATGCC 
GACAAGGAGC GCCTGGCCAT CCTGGGCATC CACAATGAGG TGTCCAAGAT TGTCCAGACC 
TACCACGTCA ATATGGCGGG CACCAACCCC TACACAACCA TCACGCCTCA GGAGATCAAT 
GGCAAATGGG ACCACGTGCG GCAGCTGGTG CCTCGGAGGG ACCAAGCTCT GACGGAGGAG 
CATGCCCGAC AGCAGCACAA TGAGAGGCTA CGCAAGCAGT TTGGAGCCCA GGCCAATGTC 
ATCGGGCCCT GGATCCAGAC CAAGATGGAG GAGATCGGGA GGATCTCCAT TGAGATGCAT 
GGGACCCTGG AGGACCAGCT CAGCCACCTG CGGCAGTATG AGAAGAGCAT CGTCAACTAC 
AAGCCAAAGA TTGATCAGCT GGAGGGCGAC CACCAGCTCA TCCAGGAGGC GCTCATCTTC 
GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATCG CCAGGACCAT CAATGAGGTA GAGAACCAGA TCCTGACCCG GGATGCCAAG 
GGCATCAGCC AGGAGCAGAT GAATGAGTTC CGGGCCTCCT TCAACCACTT TGACCGGGAT 
CACTCCGGCA CACTGGGTCC CGAGGAGTTC AAAGCCTGCC TCATCAGCTT GGGTTATGAT 
ATTGGCAACG ACCCCCAGGT ACTCGCCTCC TGCATGGAGC ACGCTCAGGG GTGGCGTCTG 
GGGCAAGAAA TAATGCGTTT TTCCTTTTTC TCTTCTCTCT GTCTGGATCT CCTGTCCCCC 
TCCTGTGGGC CATGGGGCTC GTCCTCTCCT GCTGTCTCTG TCTGCCCCAT CACTGGTCCA 
CCCTCTGCCC TCTGCTGTCT TTCATGGCAA CCTACTGGGT GCCCGCTGTC TTCCGTCCTC 
GCTGCTCTTC ATCCCATGGG TGGATTTTTG CATCTCCCTG CTCTGTCTTC CTCCTGTCTT 
TGGACATTTC CACCGATGTG TGTTCGCATC TTCTCCTATG TTCCTTTACC TATCCTGACC 
CCCAAAACCA TAAATCTCAT CCCCGTTCTG GCCATCTGTT CCTGTCTTCC TGGCCCCGGG 
CCGGCCCTTC CTCTTCCTGC CTTCCCGACC CTCCTTGTGT CTTGGTACCA CTGCCCCCCA 
CAGAAGAAGA CAGGCATGAT GGACACGGAT GATTTCCGCG CCTGCCTGAT CTCCATGGGT 
TACAACATGG GAGAAGCAGA ATTTGCCCGC ATCATGAGCA TTGTGGACCC CAACCGCCTG 
GGGGTAGTGA CATTCCAGGC CTTCATTGAC TTCATGTCCC GCGAGACAGC CGACACAGAT 
ACAGCAGACC AAGTCATGGC TTCCTTCAAG ATCCTGGCTG GGGACAAGAA CTACATTACC 
ATGGACGAGC TGCGCCGCGA GCTGCCACCC GACCAGGCTG AGTACTGCAT CGCGCGGATG 
GCCCCCTACA CCGGCCCCGA CTCCGTGCCA GGTGCTCTGG ACTACATGTC CTTCTCCACG 
GCGCTGTACG GCGAGAGTGA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877215.1
CDS	485..3631
Translation	MGLRYVLPLRPPGKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQVLASCMEHAQGWRLGQEIMRFSFFSSLCLDLLSPSCGPWGSSSPAVSVCPITGPPSALCCLSWQPTGCPLSSVLAALHPMGGFLHLPALSSSCLWTFPPMCVRIFSYVPLPILTPKTINLIPVLAICSCLPGPGPALPLPAFPTLLVSWYHCPPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_017021726.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 1 (ACTN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021726.1

ORF Insert Sequence:

ATGGGCTTGC GCTACGTCCT GCCCCTCCGA CCCCCGGGAA AGACATTCAC GGCATGGTGT 
AACTCCCACC TCCGGAAGGC GGGGACACAG ATCGAGAACA TCGAAGAGGA CTTCCGGGAT 
GGCCTGAAGC TCATGCTGCT GCTGGAGGTC ATCTCAGGTG AACGCTTGGC CAAGCCAGAG 
CGAGGCAAGA TGAGAGTGCA CAAGATCTCC AACGTCAACA AGGCCCTGGA TTTCATAGCC 
AGCAAAGGCG TCAAACTGGT GTCCATCGGA GCCGAAGAAA TCGTGGATGG GAATGTGAAG 
ATGACCCTGG GCATGATCTG GACCATCATC CTGCGCTTTG CCATCCAGGA CATCTCCGTG 
GAAGAGACTT CAGCCAAGGA AGGGCTGCTC CTGTGGTGTC AGAGAAAGAC AGCCCCTTAC 
AAAAATGTCA ACATCCAGAA CTTCCACATA AGCTGGAAGG ATGGCCTCGG CTTCTGTGCT 
TTGATCCACC GACACCGGCC CGAGCTGATT GACTACGGGA AGCTGCGGAA GGATGATCCA 
CTCACAAATC TGAATACGGC TTTTGACGTG GCAGAGAAGT ACCTGGACAT CCCCAAGATG 
CTGGATGCCG AAGACATCGT TGGAACTGCC CGACCGGATG AGAAAGCCAT CATGACTTAC 
GTGTCTAGCT TCTACCACGC CTTCTCTGGA GCCCAGAAGG CGGAGACAGC AGCCAATCGC 
ATCTGCAAGG TGTTGGCCGT CAACCAGGAG AACGAGCAGC TTATGGAAGA CTACGAGAAG 
CTGGCCAGTG ATCTGTTGGA GTGGATCCGC CGCACAATCC CGTGGCTGGA GAACCGGGTG 
CCCGAGAACA CCATGCATGC CATGCAACAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC 
CTGCACAAGC CGCCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGCTCAG CAACCGGCCT GCCTTCATGC CCTCTGAGGG CAGGATGGTC 
TCGGACATCA ACAATGCCTG GGGCTGCCTG GAGCAGGTGG AGAAGGGCTA TGAGGAGTGG 
TTGCTGAATG AGATCCGGAG GCTGGAGCGA CTGGACCACC TGGCAGAGAA GTTCCGGCAG 
AAGGCCTCCA TCCACGAGGC CTGGACTGAC GGCAAAGAGG CCATGCTGCG ACAGAAGGAC 
TATGAGACCG CCACCCTCTC GGAGATCAAG GCCCTGCTCA AGAAGCATGA GGCCTTCGAG 
AGTGACCTGG CTGCCCACCA GGACCGTGTG GAGCAGATTG CCGCCATCGC ACAGGAGCTC 
AATGAGCTGG ACTATTATGA CTCACCCAGT GTCAACGCCC GTTGCCAAAA GATCTGTGAC 
CAGTGGGACA ATCTGGGGGC CCTAACTCAG AAGCGAAGGG AAGCTCTGGA GCGGACCGAG 
AAACTGCTGG AGACCATTGA CCAGCTGTAC TTGGAGTATG CCAAGCGGGC TGCACCCTTC 
AACAACTGGA TGGAGGGGGC CATGGAGGAC CTGCAGGACA CCTTCATTGT GCACACCATT 
GAGGAGATCC AGGGACTGAC CACAGCCCAT GAGCAGTTCA AGGCCACCCT CCCTGATGCC 
GACAAGGAGC GCCTGGCCAT CCTGGGCATC CACAATGAGG TGTCCAAGAT TGTCCAGACC 
TACCACGTCA ATATGGCGGG CACCAACCCC TACACAACCA TCACGCCTCA GGAGATCAAT 
GGCAAATGGG ACCACGTGCG GCAGCTGGTG CCTCGGAGGG ACCAAGCTCT GACGGAGGAG 
CATGCCCGAC AGCAGCACAA TGAGAGGCTA CGCAAGCAGT TTGGAGCCCA GGCCAATGTC 
ATCGGGCCCT GGATCCAGAC CAAGATGGAG GAGATCGGGA GGATCTCCAT TGAGATGCAT 
GGGACCCTGG AGGACCAGCT CAGCCACCTG CGGCAGTATG AGAAGAGCAT CGTCAACTAC 
AAGCCAAAGA TTGATCAGCT GGAGGGCGAC CACCAGCTCA TCCAGGAGGC GCTCATCTTC 
GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATCG CCAGGACCAT CAATGAGGTA GAGAACCAGA TCCTGACCCG GGATGCCAAG 
GGCATCAGCC AGGAGCAGAT GAATGAGTTC CGGGCCTCCT TCAACCACTT TGACCGGGAT 
CACTCCGGCA CACTGGGTCC CGAGGAGTTC AAAGCCTGCC TCATCAGCTT GGGTTATGAT 
ATTGGCAACG ACCCCCAGGT ACTCGCCTCC TGCATGGAGC ACGCTCAGGG GTGGCGTCTG 
GGGCAAGAAA TAATGCGTTT TTCCTTTTTC TCTTCTCTCT GTCTGGATCT CCTGTCCCCC 
TCCTGTGGGC CATGGGGCTC GTCCTCTCCT GCTGTCTCTG TCTGCCCCAT CACTGGTCCA 
CCCTCTGCCC TCTGCTGTCT TTCATGGCAA CCTACTGGGT GCCCGCTGTC TTCCGTCCTC 
GCTGCTCTTC ATCCCATGGG TGGATTTTTG CATCTCCCTG CTCTGTCTTC CTCCTGTCTT 
TGGACATTTC CACCGATGTG TGTTCGCATC TTCTCCTATG TTCCTTTACC TATCCTGACC 
CCCAAAACCA TAAATCTCAT CCCCGTTCTG GCCATCTGTT CCTGTCTTCC TGGCCCCGGG 
CCGGCCCTTC CTCTTCCTGC CTTCCCGACC CTCCTTGTGT CTTGGTACCA CTGCCCCCCA 
CAGAAGAAGA CAGGCATGAT GGACACGGAT GATTTCCGCG CCTGCCTGAT CTCCATGGGT 
TACAACATGG GAGAAGCAGA ATTTGCCCGC ATCATGAGCA TTGTGGACCC CAACCGCCTG 
GGGGTAGTGA CATTCCAGGC CTTCATTGAC TTCATGTCCC GCGAGACAGC CGACACAGAT 
ACAGCAGACC AAGTCATGGC TTCCTTCAAG ATCCTGGCTG GGGACAAGAA CTACATTACC 
ATGGACGAGC TGCGCCGCGA GCTGCCACCC GACCAGGCTG AGTACTGCAT CGCGCGGATG 
GCCCCCTACA CCGGCCCCGA CTCCGTGCCA GGTGCTCTGG ACTACATGTC CTTCTCCACG 
GCGCTGTACG GCGAGAGTGA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ACTN1
Entrez Gene ID	87
Full Name	actinin alpha 1
Synonyms	BDPLT15
General protein information	Preferred Names actinin alpha 1 Names alpha-actinin-1 F-actin cross-linking protein actinin 1 smooth muscle alpha-actinin cytoskeletal isoform non-muscle alpha-actinin-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q24.1\|14q22-q24
Summary	Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	102575
Disorder Html:	Bleeding disorder, platelet-type, 15, 615193 (3)

Pan troglodytes (chimpanzee)	ACTN1	XP_001139826.3
Mus musculus (house mouse)	Actn1	NP_598917.1
Homo sapiens (human)	ACTN1	NP_001123476.1
Canis lupus familiaris (dog)	ACTN1	XP_853103.1
Bos taurus (cattle)	ACTN1	NP_001030428.1
Xenopus tropicalis (tropical clawed frog)	actn1	NP_001072666.1
Rattus norvegicus (Norway rat)	Actn1	NP_112267.1
Gallus gallus (chicken)	ACTN1	NP_989458.1
Danio rerio (zebrafish)	actn1	NP_001161758.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ACTN1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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