EIF3A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF3A
Entrez Gene ID	8661
Full Name	eukaryotic translation initiation factor 3 subunit A
Synonyms	EIF3,EIF3S10,P167,TIF32,eIF3-p170,eIF3-theta,p180,p185
General protein information	Preferred Names eukaryotic translation initiation factor 3 subunit A Names eukaryotic translation initiation factor 3 subunit A EIF3, p180 subunit centrosomin homolog cytoplasmic protein p167 eIF-3-theta eIF3 p167 eIF3 p180 eIF3 p185 eukaryotic translation initiation factor 3 subunit 10 eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD) eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD) eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa eukaryotic translation initiation factor 3, subunit 10, 170kD
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q26.11
Disorder MIM:	602039

mRNA and Protein(s)

mRNA	Protein	Name
NM_003750.2	NP_003741.1	eukaryotic translation initiation factor 3 subunit A
NM_003750.3	NP_003741.1	eukaryotic translation initiation factor 3 subunit A
NM_003750.4	NP_003741.1	eukaryotic translation initiation factor 3 subunit A

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF3A	NP_003741.1
Pan troglodytes (chimpanzee)	EIF3A	XP_001153575.1
Mus musculus (house mouse)	Eif3a	NP_034253.3
Oryza sativa (rice)	Os01g0120800	NP_001041871.1
Danio rerio (zebrafish)	eif3s10	NP_956114.2
Anopheles gambiae (African malaria mosquito)	EIF3A_ANOGA	XP_312628.5
Caenorhabditis elegans (roundworm)	egl-45	NP_498698.1
Saccharomyces cerevisiae (baker's yeast)	RPG1	NP_009635.1
Arabidopsis thaliana (thale cress)	EIF3A	NP_192881.1
Bos taurus (cattle)	EIF3A	NP_001193243.1
Drosophila melanogaster (fruit fly)	eIF3-S10	NP_649470.2
Kluyveromyces lactis	KLLA0C14498g	XP_452849.1
Neurospora crassa	NCU00040	XP_956151.2
Rattus norvegicus (Norway rat)	Eif3a	NP_001040552.2
Gallus gallus (chicken)	EIF3A	XP_421787.3
Schizosaccharomyces pombe (fission yeast)	tif32	NP_596379.1
Magnaporthe oryzae (rice blast fungus)	MGG_10192	XP_003713822.1
Canis lupus familiaris (dog)	EIF3A	XP_852131.1
Eremothecium gossypii	AGOS_ABL065W	NP_982882.1
Xenopus tropicalis (tropical clawed frog)	eif3a	NP_001096173.1

Gene Ontology
Bibliography

Related articles in PubMed

Knockdown of elF3a inhibits TGF??1?induced extracellular matrix protein expression in keloid fibroblasts.
Li T, Zhao J
Molecular medicine reports(2017 Dec)

Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle.
Val??ek LS, Zeman J, Wagner S, Beznoskov? P, Pavl?kov? Z, Mohammad MP, Hronov? V, Herrmannov? A, Hashem Y, Guni?ov? S
Nucleic acids research45(19)10948-10968(2017 Nov)

The eIF3a Arg803Lys genetic polymorphism is associated with susceptibility to and chemoradiotherapy efficacy in cervical carcinoma.
Xu JZ, Wen F, Wang XR
The Kaohsiung journal of medical sciences33(4)187-194(2017 Apr)

MiR-488 inhibits proliferation and cisplatin sensibility in non-small-cell lung cancer (NSCLC) cells by activating the eIF3a-mediated NER signaling pathway.
Fang C, Chen YX, Wu NY, Yin JY, Li XP, Huang HS, Zhang W, Zhou HH, Liu ZQ
Scientific reports740384(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies suggest that eukaryotic initiation factor eIF3, which coordinates the progress of most of the initiation steps, does not come off the initiation complex upon subunit joining, but instead it remains bound to 80S ribosomes.
Title: Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle.

The eIF3a Arg803Lys C>T polymorphism is connected with a higher susceptibility to cervical carcinoma and may affect chemoradiotherapy efficacy in and prognosis of cervical carcinoma.
Title: The eIF3a Arg803Lys genetic polymorphism is associated with susceptibility to and chemoradiotherapy efficacy in cervical carcinoma.

interaction involves the first FF motif of p190A and the winged helix/PCI domain of eIF3A, is enhanced by serum stimulation and reduced by phosphatase treatment
Title: Interaction of p190A RhoGAP with eIF3A and Other Translation Preinitiation Factors Suggests a Role in Protein Biosynthesis.

The human eIF3b and octameric eIF3a subunits serve as the nucleation core around which other subunits assemble in an ordered way into two interconnected modules: the yeast-like core and the octamer.
Title: Human eIF3b and eIF3a serve as the nucleation core for the assembly of eIF3 into two interconnected modules: the yeast-like core and the octamer.

DHX29 and eIF3 cooperate in scanning on structured mRNAs. Our findings support previous genetic data on the role of eIF3 during scanning
Title: DHX29 and eIF3 cooperate in ribosomal scanning on structured mRNAs during translation initiation.

The following EIF3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17434	NM_003750.4 Latest version!	Homo sapiens eukaryotic translation initiation factor 3 subunit A (EIF3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
View Old Accession Versions >>
OHu17434	NM_003750.2	Homo sapiens eukaryotic translation initiation factor 3 subunit A (EIF3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu17434	NM_003750.3	Homo sapiens eukaryotic translation initiation factor 3 subunit A (EIF3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF3A ( NM_003750.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003741.1 Homo sapiens eukaryotic translation initiation factor 3 subunit A (EIF3A), mRNA.

Price & Availability↑

CloneID	OHu17434
Clone ID Related Accession (Same CDS sequence)	NM_003750.2 , NM_003750.3 , NM_003750.4
Accession Version	NM_003750.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-27
Organism	Homo sapiens(Human)
Product	eukaryotic translation initiation factor 3 subunit A
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AU279600.1, D50929.1 and AL355598.26. On Dec 8, 2005 this sequence version replaced NM_003750.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.255383.1, SRR1660809.10339.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGGCCT ATTTTCAGAG GCCGGAAAAT GCCCTCAAAC GCGCCAACGA ATTTCTTGAG 
GTTGGCAAAA AGCAGCCTGC TCTGGATGTT CTTTATGATG TTATGAAAAG TAAAAAACAT 
AGAACATGGC AAAAGATACA CGAACCAATT ATGTTGAAAT ACTTGGAACT TTGCGTGGAT 
CTTCGCAAGA GCCACTTGGC AAAGGAGGGG TTATACCAGT ATAAGAACAT TTGTCAACAG 
GTGAACATAA AATCTCTGGA GGATGTTGTT AGGGCATATT TGAAAATGGC AGAGGAAAAA 
ACTGAAGCTG CTAAAGAAGA ATCTCAGCAG ATGGTCTTAG ATATAGAGGA TCTAGATAAT 
ATTCAAACTC CTGAGAGTGT TCTCCTAAGT GCTGTAAGTG GTGAAGACAC TCAGGATCGT 
ACTGACAGAT TACTTTTAAC TCCATGGGTT AAATTCCTGT GGGAGTCTTA CAGGCAGTGT 
TTGGACCTTC TTAGAAACAA TTCTAGAGTA GAGCGCCTGT ACCATGATAT TGCCCAGCAA 
GCTTTCAAAT TCTGCCTCCA ATACACGCGT AAGGCTGAAT TCCGTAAACT GTGTGACAAT 
TTGAGAATGC ACTTATCGCA GATTCAGCGC CACCATAACC AAAGTACGGC AATCAATCTT 
AATAATCCAG AGAGCCAGTC CATGCATTTG GAAACCAGAC TTGTTCAGCT GGACAGTGCT 
ATCAGCATGG AATTGTGGCA GGAAGCATTC AAAGCTGTGG AAGATATTCA CGGGCTATTC 
TCCTTGTCTA AAAAACCACC TAAACCTCAG TTGATGGCAA ATTACTATAA CAAAGTCTCA 
ACTGTGTTTT GGAAATCTGG AAATGCTCTT TTTCATGCAT CTACACTCCA TCGTCTTTAC 
CATCTCTCTA GAGAAATGAG AAAGAATCTC ACACAAGATG AGATGCAAAG AATGTCTACT 
AGAGTCCTTT TAGCCACTCT TTCCATCCCT ATTACTCCTG AGCGTACGGA TATTGCTCGA 
CTTCTGGATA TGGATGGCAT TATAGTTGAA AAACAGCGTC GCCTTGCAAC ACTACTAGGT 
CTTCAAGCCC CACCGACACG AATTGGCCTT ATTAATGATA TGGTCAGATT TAATGTACTA 
CAATATGTTG TCCCAGAAGT GAAAGACCTT TACAATTGGC TTGAAGTAGA ATTTAACCCA 
TTAAAACTCT GTGAGCGAGT CACAAAGGTT CTAAATTGGG TTAGGGAACA ACCTGAAAAG 
GAACCGGAAT TGCAGCAGTA TGTGCCACAA CTGCAAAACA ACACCATCCT CCGCCTTCTG 
CAGCAGGTGT CACAGATTTA TCAGAGCATT GAGTTTTCTC GTTTGACTTC TTTGGTTCCT 
TTTGTTGATG CTTTCCAACT GGAACGGGCC ATAGTAGATG CAGCCAGGCA TTGCGACTTG 
CAGGTTCGTA TTGATCACAC TTCTCGGACC CTGAGTTTTG GATCTGATTT GAATTATGCT 
ACTCGAGAAG ATGCTCCGAT TGGTCCTCAT TTGCAAAGCA TGCCTTCAGA GCAGATAAGA 
AACCAGCTGA CAGCCATGTC CTCAGTACTT GCAAAAGCAC TTGAAGTCAT TAAACCAGCT 
CATATACTGC AAGAGAAAGA AGAACAGCAT CAGTTGGCTG TCACTGCATA CCTTAAAAAT 
TCACGAAAAG AGCACCAGCG GATCCTGGCT CGCCGCCAGA CAATTGAGGA GAGAAAAGAG 
CGCCTTGAGA GTCTGAATAT TCAGCGTGAG AAAGAAGAAT TGGAACAGAG GGAAGCTGAA 
CTCCAGAAAG TGCGGAAGGC TGAGGAAGAG AGGCTGCGCC AGGAAGCAAA GGAGAGAGAG 
AAGGAGCGTA TCTTACAGGA ACATGAACAA ATCAAAAAGA AAACTGTCCG AGAGCGTTTG 
GAGCAGATCA AGAAAACAGA ACTGGGTGCC AAAGCATTCA AAGATATTGA TATTGAAGAC 
CTTGAGGAAT TGGATCCAGA TTTTATCATG GCTAAACAGG TTGAACAACT GGAGAAAGAA 
AAGAAAGAAC TTCAAGAACG CCTAAAGAAT CAAGAAAAGA AGATTGACTA TTTTGAAAGA 
GCCAAACGTT TGGAAGAAAT TCCTTTGATA AAGAGCGCTT ACGAGGAACA GAGAATTAAA 
GACATGGATC TGTGGGAGCA ACAAGAGGAA GAAAGAATTA CTACAATGCA GCTAGAACGT 
GAAAAGGCTC TTGAACATAA GAATCGAATG TCACGAATGC TTGAAGACAG AGATTTATTC 
GTAATGCGAC TCAAAGCTGC ACGGCAGTCT GTTTATGAGG AAAAACTTAA ACAGTTTGAA 
GAGCGATTAG CAGAAGAAAG GCATAATCGA TTGGAAGAAC GGAAAAGGCA GCGTAAAGAA 
GAACGCAGGA TAACATACTA TAGAGAAAAA GAAGAGGAGG AGCAGAGAAG GGCAGAAGAA 
CAAATGCTAA AAGAGCGGGA AGAGAGAGAG CGCGCCGAAC GAGCAAAACG CGAGGAAGAG 
CTACGAGAGT ATCAGGAGCG GGTGAAGAAA TTAGAAGAAG TGGAAAGGAA AAAACGCCAA 
AGGGAGTTGG AAATTGAAGA ACGAGAACGG CGTAGAGAGG AAGAGAGAAG ACTTGGCGAT 
AGTTCCCTTT CTAGAAAGGA CTCTCGTTGG GGAGATAGAG ATTCAGAAGG CACCTGGAGA 
AAAGGACCTG AAGCAGATTC TGAGTGGAGA AGAGGCCCGC CAGAGAAGGA GTGGAGACGT 
GGAGAAGGGC GAGATGAGGA CAGGTCTCAT AGAAGAGATG AAGAGCGGCC CCGGCGTCTG 
GGGGATGATG AAGATAGAGA GCCCTCTCTT AGACCAGACG ATGATCGGGT TCCCCGGCGT 
GGCATGGATG ATGACAGAGG CCCTAGACGT GGTCCTGAGG AAGATAGGTT CTCTCGTCGT 
GGGGCAGACG ATGACCGGCC TTCCTGGCGT AACACAGATG ATGACAGGCC TCCCAGACGA 
ATTGCCGATG AAGACAGGGG AAACTGGCGT CATGCGGATG ATGACAGACC ACCTAGACGA 
GGACTGGATG AGGACAGAGG AAGCTGGCGA ACAGCTGATG AGGACAGAGG ACCAAGACGT 
GGGATGGATG ATGACCGGGG GCCGAGGCGA GGAGGCGCTG ATGATGAGCG ATCATCCTGG 
CGTAATGCTG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGCATGG ATGATGACCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG AGGACCTTGG 
AGGAACGCCG ATGATGACAG AATTCCCAGG CGTGGTGCAG AGGATGACAG GGGCCCTTGG 
AGAAACATGG ATGATGATCG CCTTTCAAGA CGTGCTGATG ATGATCGGTT TCCCAGACGG 
GGTGATGACT CAAGACCTGG TCCTTGGAGA CCATTAGTCA AGCCAGGTGG ATGGAGAGAG 
AAAGAAAAAG CCAGAGAGGA GAGCTGGGGT CCACCTCGAG AATCAAGGCC ATCAGAAGAA 
CGTGAATGGG ACAGAGAAAA AGAAAGGGAC AGAGATAATC AAGATCGGGA GGAGAATGAC 
AAGGACCCTG AGAGAGAAAG GGACAGAGAG AGAGATGTGG ATCGAGAGGA TCGCTTCAGA 
AGACCTAGGG ATGAAGGTGG CTGGAGAAGA GGACCAGCTG AGGAATCTTC AAGCTGGAGA 
GACTCAAGTC GCCGGGACGA TAGGGATAGG GATGACCGTC GCCGTGAGAG GGATGACCGG 
CGTGATCTAA GAGAAAGACG AGATCTAAGA GACGACAGGG ACCGAAGAGG ACCTCCACTC 
AGATCAGAAC GTGAAGAAGT AAGTTCTTGG AGACGTGCTG ATGACAGGAA AGATGACCGG 
GTGGAAGAGC GGGACCCTCC TCGTCGAGTT CCTCCCCCAG CTCTTTCAAG AGACCGAGAA 
AGAGACCGAG ACCGAGAAAG AGAAGGTGAA AAAGAGAAGG CCTCATGGAG AGCTGAGAAA 
GATAGGGAAT CTCTCCGTCG TACTAAAAAT GAGACTGATG AAGATGGATG GACCACAGTA 
CGACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003741.1
CDS	147..4295
Translation	MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRIKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDSSLSRKDSRWGDRDSEGTWRKGPEADSEWRRGPPEKEWRRGEGRDEDRSHRRDEERPRRLGDDEDREPSLRPDDDRVPRRGMDDDRGPRRGPEEDRFSRRGADDDRPSWRNTDDDRPPRRIADEDRGNWRHADDDRPPRRGLDEDRGSWRTADEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPWRNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPRRGDDSRPGPWRPLVKPGGWREKEKAREESWGPPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWRDSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRVPPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR

Target ORF information:

RefSeq Version	NM_003750.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens eukaryotic translation initiation factor 3 subunit A (EIF3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003750.2

ORF Insert Sequence:

ATGCCGGCCT ATTTTCAGAG GCCGGAAAAT GCCCTCAAAC GCGCCAACGA ATTTCTTGAG 
GTTGGCAAAA AGCAGCCTGC TCTGGATGTT CTTTATGATG TTATGAAAAG TAAAAAACAT 
AGAACATGGC AAAAGATACA CGAACCAATT ATGTTGAAAT ACTTGGAACT TTGCGTGGAT 
CTTCGCAAGA GCCACTTGGC AAAGGAGGGG TTATACCAGT ATAAGAACAT TTGTCAACAG 
GTGAACATAA AATCTCTGGA GGATGTTGTT AGGGCATATT TGAAAATGGC AGAGGAAAAA 
ACTGAAGCTG CTAAAGAAGA ATCTCAGCAG ATGGTCTTAG ATATAGAGGA TCTAGATAAT 
ATTCAAACTC CTGAGAGTGT TCTCCTAAGT GCTGTAAGTG GTGAAGACAC TCAGGATCGT 
ACTGACAGAT TACTTTTAAC TCCATGGGTT AAATTCCTGT GGGAGTCTTA CAGGCAGTGT 
TTGGACCTTC TTAGAAACAA TTCTAGAGTA GAGCGCCTGT ACCATGATAT TGCCCAGCAA 
GCTTTCAAAT TCTGCCTCCA ATACACGCGT AAGGCTGAAT TCCGTAAACT GTGTGACAAT 
TTGAGAATGC ACTTATCGCA GATTCAGCGC CACCATAACC AAAGTACGGC AATCAATCTT 
AATAATCCAG AGAGCCAGTC CATGCATTTG GAAACCAGAC TTGTTCAGCT GGACAGTGCT 
ATCAGCATGG AATTGTGGCA GGAAGCATTC AAAGCTGTGG AAGATATTCA CGGGCTATTC 
TCCTTGTCTA AAAAACCACC TAAACCTCAG TTGATGGCAA ATTACTATAA CAAAGTCTCA 
ACTGTGTTTT GGAAATCTGG AAATGCTCTT TTTCATGCAT CTACACTCCA TCGTCTTTAC 
CATCTCTCTA GAGAAATGAG AAAGAATCTC ACACAAGATG AGATGCAAAG AATGTCTACT 
AGAGTCCTTT TAGCCACTCT TTCCATCCCT ATTACTCCTG AGCGTACGGA TATTGCTCGA 
CTTCTGGATA TGGATGGCAT TATAGTTGAA AAACAGCGTC GCCTTGCAAC ACTACTAGGT 
CTTCAAGCCC CACCGACACG AATTGGCCTT ATTAATGATA TGGTCAGATT TAATGTACTA 
CAATATGTTG TCCCAGAAGT GAAAGACCTT TACAATTGGC TTGAAGTAGA ATTTAACCCA 
TTAAAACTCT GTGAGCGAGT CACAAAGGTT CTAAATTGGG TTAGGGAACA ACCTGAAAAG 
GAACCGGAAT TGCAGCAGTA TGTGCCACAA CTGCAAAACA ACACCATCCT CCGCCTTCTG 
CAGCAGGTGT CACAGATTTA TCAGAGCATT GAGTTTTCTC GTTTGACTTC TTTGGTTCCT 
TTTGTTGATG CTTTCCAACT GGAACGGGCC ATAGTAGATG CAGCCAGGCA TTGCGACTTG 
CAGGTTCGTA TTGATCACAC TTCTCGGACC CTGAGTTTTG GATCTGATTT GAATTATGCT 
ACTCGAGAAG ATGCTCCGAT TGGTCCTCAT TTGCAAAGCA TGCCTTCAGA GCAGATAAGA 
AACCAGCTGA CAGCCATGTC CTCAGTACTT GCAAAAGCAC TTGAAGTCAT TAAACCAGCT 
CATATACTGC AAGAGAAAGA AGAACAGCAT CAGTTGGCTG TCACTGCATA CCTTAAAAAT 
TCACGAAAAG AGCACCAGCG GATCCTGGCT CGCCGCCAGA CAATTGAGGA GAGAAAAGAG 
CGCCTTGAGA GTCTGAATAT TCAGCGTGAG AAAGAAGAAT TGGAACAGAG GGAAGCTGAA 
CTCCAGAAAG TGCGGAAGGC TGAGGAAGAG AGGCTGCGCC AGGAAGCAAA GGAGAGAGAG 
AAGGAGCGTA TCTTACAGGA ACATGAACAA ATCAAAAAGA AAACTGTCCG AGAGCGTTTG 
GAGCAGATCA AGAAAACAGA ACTGGGTGCC AAAGCATTCA AAGATATTGA TATTGAAGAC 
CTTGAGGAAT TGGATCCAGA TTTTATCATG GCTAAACAGG TTGAACAACT GGAGAAAGAA 
AAGAAAGAAC TTCAAGAACG CCTAAAGAAT CAAGAAAAGA AGATTGACTA TTTTGAAAGA 
GCCAAACGTT TGGAAGAAAT TCCTTTGATA AAGAGCGCTT ACGAGGAACA GAGAATTAAA 
GACATGGATC TGTGGGAGCA ACAAGAGGAA GAAAGAATTA CTACAATGCA GCTAGAACGT 
GAAAAGGCTC TTGAACATAA GAATCGAATG TCACGAATGC TTGAAGACAG AGATTTATTC 
GTAATGCGAC TCAAAGCTGC ACGGCAGTCT GTTTATGAGG AAAAACTTAA ACAGTTTGAA 
GAGCGATTAG CAGAAGAAAG GCATAATCGA TTGGAAGAAC GGAAAAGGCA GCGTAAAGAA 
GAACGCAGGA TAACATACTA TAGAGAAAAA GAAGAGGAGG AGCAGAGAAG GGCAGAAGAA 
CAAATGCTAA AAGAGCGGGA AGAGAGAGAG CGCGCCGAAC GAGCAAAACG CGAGGAAGAG 
CTACGAGAGT ATCAGGAGCG GGTGAAGAAA TTAGAAGAAG TGGAAAGGAA AAAACGCCAA 
AGGGAGTTGG AAATTGAAGA ACGAGAACGG CGTAGAGAGG AAGAGAGAAG ACTTGGCGAT 
AGTTCCCTTT CTAGAAAGGA CTCTCGTTGG GGAGATAGAG ATTCAGAAGG CACCTGGAGA 
AAAGGACCTG AAGCAGATTC TGAGTGGAGA AGAGGCCCGC CAGAGAAGGA GTGGAGACGT 
GGAGAAGGGC GAGATGAGGA CAGGTCTCAT AGAAGAGATG AAGAGCGGCC CCGGCGTCTG 
GGGGATGATG AAGATAGAGA GCCCTCTCTT AGACCAGACG ATGATCGGGT TCCCCGGCGT 
GGCATGGATG ATGACAGAGG CCCTAGACGT GGTCCTGAGG AAGATAGGTT CTCTCGTCGT 
GGGGCAGACG ATGACCGGCC TTCCTGGCGT AACACAGATG ATGACAGGCC TCCCAGACGA 
ATTGCCGATG AAGACAGGGG AAACTGGCGT CATGCGGATG ATGACAGACC ACCTAGACGA 
GGACTGGATG AGGACAGAGG AAGCTGGCGA ACAGCTGATG AGGACAGAGG ACCAAGACGT 
GGGATGGATG ATGACCGGGG GCCGAGGCGA GGAGGCGCTG ATGATGAGCG ATCATCCTGG 
CGTAATGCTG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGCATGG ATGATGACCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG AGGACCTTGG 
AGGAACGCCG ATGATGACAG AATTCCCAGG CGTGGTGCAG AGGATGACAG GGGCCCTTGG 
AGAAACATGG ATGATGATCG CCTTTCAAGA CGTGCTGATG ATGATCGGTT TCCCAGACGG 
GGTGATGACT CAAGACCTGG TCCTTGGAGA CCATTAGTCA AGCCAGGTGG ATGGAGAGAG 
AAAGAAAAAG CCAGAGAGGA GAGCTGGGGT CCACCTCGAG AATCAAGGCC ATCAGAAGAA 
CGTGAATGGG ACAGAGAAAA AGAAAGGGAC AGAGATAATC AAGATCGGGA GGAGAATGAC 
AAGGACCCTG AGAGAGAAAG GGACAGAGAG AGAGATGTGG ATCGAGAGGA TCGCTTCAGA 
AGACCTAGGG ATGAAGGTGG CTGGAGAAGA GGACCAGCTG AGGAATCTTC AAGCTGGAGA 
GACTCAAGTC GCCGGGACGA TAGGGATAGG GATGACCGTC GCCGTGAGAG GGATGACCGG 
CGTGATCTAA GAGAAAGACG AGATCTAAGA GACGACAGGG ACCGAAGAGG ACCTCCACTC 
AGATCAGAAC GTGAAGAAGT AAGTTCTTGG AGACGTGCTG ATGACAGGAA AGATGACCGG 
GTGGAAGAGC GGGACCCTCC TCGTCGAGTT CCTCCCCCAG CTCTTTCAAG AGACCGAGAA 
AGAGACCGAG ACCGAGAAAG AGAAGGTGAA AAAGAGAAGG CCTCATGGAG AGCTGAGAAA 
GATAGGGAAT CTCTCCGTCG TACTAAAAAT GAGACTGATG AAGATGGATG GACCACAGTA 
CGACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF3A ( NM_003750.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003741.1 Homo sapiens eukaryotic translation initiation factor 3 subunit A (EIF3A), mRNA.

Price & Availability↑

CloneID	OHu17434
Clone ID Related Accession (Same CDS sequence)	NM_003750.2 , NM_003750.3 , NM_003750.4
Accession Version	NM_003750.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 3 subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AL355598.26 and AL157788.16. On Aug 29, 2017 this sequence version replaced NM_003750.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.10339.1, SRR1660807.136767.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGGCCT ATTTTCAGAG GCCGGAAAAT GCCCTCAAAC GCGCCAACGA ATTTCTTGAG 
GTTGGCAAAA AGCAGCCTGC TCTGGATGTT CTTTATGATG TTATGAAAAG TAAAAAACAT 
AGAACATGGC AAAAGATACA CGAACCAATT ATGTTGAAAT ACTTGGAACT TTGCGTGGAT 
CTTCGCAAGA GCCACTTGGC AAAGGAGGGG TTATACCAGT ATAAGAACAT TTGTCAACAG 
GTGAACATAA AATCTCTGGA GGATGTTGTT AGGGCATATT TGAAAATGGC AGAGGAAAAA 
ACTGAAGCTG CTAAAGAAGA ATCTCAGCAG ATGGTCTTAG ATATAGAGGA TCTAGATAAT 
ATTCAAACTC CTGAGAGTGT TCTCCTAAGT GCTGTAAGTG GTGAAGACAC TCAGGATCGT 
ACTGACAGAT TACTTTTAAC TCCATGGGTT AAATTCCTGT GGGAGTCTTA CAGGCAGTGT 
TTGGACCTTC TTAGAAACAA TTCTAGAGTA GAGCGCCTGT ACCATGATAT TGCCCAGCAA 
GCTTTCAAAT TCTGCCTCCA ATACACGCGT AAGGCTGAAT TCCGTAAACT GTGTGACAAT 
TTGAGAATGC ACTTATCGCA GATTCAGCGC CACCATAACC AAAGTACGGC AATCAATCTT 
AATAATCCAG AGAGCCAGTC CATGCATTTG GAAACCAGAC TTGTTCAGCT GGACAGTGCT 
ATCAGCATGG AATTGTGGCA GGAAGCATTC AAAGCTGTGG AAGATATTCA CGGGCTATTC 
TCCTTGTCTA AAAAACCACC TAAACCTCAG TTGATGGCAA ATTACTATAA CAAAGTCTCA 
ACTGTGTTTT GGAAATCTGG AAATGCTCTT TTTCATGCAT CTACACTCCA TCGTCTTTAC 
CATCTCTCTA GAGAAATGAG AAAGAATCTC ACACAAGATG AGATGCAAAG AATGTCTACT 
AGAGTCCTTT TAGCCACTCT TTCCATCCCT ATTACTCCTG AGCGTACGGA TATTGCTCGA 
CTTCTGGATA TGGATGGCAT TATAGTTGAA AAACAGCGTC GCCTTGCAAC ACTACTAGGT 
CTTCAAGCCC CACCGACACG AATTGGCCTT ATTAATGATA TGGTCAGATT TAATGTACTA 
CAATATGTTG TCCCAGAAGT GAAAGACCTT TACAATTGGC TTGAAGTAGA ATTTAACCCA 
TTAAAACTCT GTGAGCGAGT CACAAAGGTT CTAAATTGGG TTAGGGAACA ACCTGAAAAG 
GAACCGGAAT TGCAGCAGTA TGTGCCACAA CTGCAAAACA ACACCATCCT CCGCCTTCTG 
CAGCAGGTGT CACAGATTTA TCAGAGCATT GAGTTTTCTC GTTTGACTTC TTTGGTTCCT 
TTTGTTGATG CTTTCCAACT GGAACGGGCC ATAGTAGATG CAGCCAGGCA TTGCGACTTG 
CAGGTTCGTA TTGATCACAC TTCTCGGACC CTGAGTTTTG GATCTGATTT GAATTATGCT 
ACTCGAGAAG ATGCTCCGAT TGGTCCTCAT TTGCAAAGCA TGCCTTCAGA GCAGATAAGA 
AACCAGCTGA CAGCCATGTC CTCAGTACTT GCAAAAGCAC TTGAAGTCAT TAAACCAGCT 
CATATACTGC AAGAGAAAGA AGAACAGCAT CAGTTGGCTG TCACTGCATA CCTTAAAAAT 
TCACGAAAAG AGCACCAGCG GATCCTGGCT CGCCGCCAGA CAATTGAGGA GAGAAAAGAG 
CGCCTTGAGA GTCTGAATAT TCAGCGTGAG AAAGAAGAAT TGGAACAGAG GGAAGCTGAA 
CTCCAGAAAG TGCGGAAGGC TGAGGAAGAG AGGCTGCGCC AGGAAGCAAA GGAGAGAGAG 
AAGGAGCGTA TCTTACAGGA ACATGAACAA ATCAAAAAGA AAACTGTCCG AGAGCGTTTG 
GAGCAGATCA AGAAAACAGA ACTGGGTGCC AAAGCATTCA AAGATATTGA TATTGAAGAC 
CTTGAGGAAT TGGATCCAGA TTTTATCATG GCTAAACAGG TTGAACAACT GGAGAAAGAA 
AAGAAAGAAC TTCAAGAACG CCTAAAGAAT CAAGAAAAGA AGATTGACTA TTTTGAAAGA 
GCCAAACGTT TGGAAGAAAT TCCTTTGATA AAGAGCGCTT ACGAGGAACA GAGAATTAAA 
GACATGGATC TGTGGGAGCA ACAAGAGGAA GAAAGAATTA CTACAATGCA GCTAGAACGT 
GAAAAGGCTC TTGAACATAA GAATCGAATG TCACGAATGC TTGAAGACAG AGATTTATTC 
GTAATGCGAC TCAAAGCTGC ACGGCAGTCT GTTTATGAGG AAAAACTTAA ACAGTTTGAA 
GAGCGATTAG CAGAAGAAAG GCATAATCGA TTGGAAGAAC GGAAAAGGCA GCGTAAAGAA 
GAACGCAGGA TAACATACTA TAGAGAAAAA GAAGAGGAGG AGCAGAGAAG GGCAGAAGAA 
CAAATGCTAA AAGAGCGGGA AGAGAGAGAG CGCGCCGAAC GAGCAAAACG CGAGGAAGAG 
CTACGAGAGT ATCAGGAGCG GGTGAAGAAA TTAGAAGAAG TGGAAAGGAA AAAACGCCAA 
AGGGAGTTGG AAATTGAAGA ACGAGAACGG CGTAGAGAGG AAGAGAGAAG ACTTGGCGAT 
AGTTCCCTTT CTAGAAAGGA CTCTCGTTGG GGAGATAGAG ATTCAGAAGG CACCTGGAGA 
AAAGGACCTG AAGCAGATTC TGAGTGGAGA AGAGGCCCGC CAGAGAAGGA GTGGAGACGT 
GGAGAAGGGC GAGATGAGGA CAGGTCTCAT AGAAGAGATG AAGAGCGGCC CCGGCGTCTG 
GGGGATGATG AAGATAGAGA GCCCTCTCTT AGACCAGACG ATGATCGGGT TCCCCGGCGT 
GGCATGGATG ATGACAGAGG CCCTAGACGT GGTCCTGAGG AAGATAGGTT CTCTCGTCGT 
GGGGCAGACG ATGACCGGCC TTCCTGGCGT AACACAGATG ATGACAGGCC TCCCAGACGA 
ATTGCCGATG AAGACAGGGG AAACTGGCGT CATGCGGATG ATGACAGACC ACCTAGACGA 
GGACTGGATG AGGACAGAGG AAGCTGGCGA ACAGCTGATG AGGACAGAGG ACCAAGACGT 
GGGATGGATG ATGACCGGGG GCCGAGGCGA GGAGGCGCTG ATGATGAGCG ATCATCCTGG 
CGTAATGCTG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGCATGG ATGATGACCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG AGGACCTTGG 
AGGAACGCCG ATGATGACAG AATTCCCAGG CGTGGTGCAG AGGATGACAG GGGCCCTTGG 
AGAAACATGG ATGATGATCG CCTTTCAAGA CGTGCTGATG ATGATCGGTT TCCCAGACGG 
GGTGATGACT CAAGACCTGG TCCTTGGAGA CCATTAGTCA AGCCAGGTGG ATGGAGAGAG 
AAAGAAAAAG CCAGAGAGGA GAGCTGGGGT CCACCTCGAG AATCAAGGCC ATCAGAAGAA 
CGTGAATGGG ACAGAGAAAA AGAAAGGGAC AGAGATAATC AAGATCGGGA GGAGAATGAC 
AAGGACCCTG AGAGAGAAAG GGACAGAGAG AGAGATGTGG ATCGAGAGGA TCGCTTCAGA 
AGACCTAGGG ATGAAGGTGG CTGGAGAAGA GGACCAGCTG AGGAATCTTC AAGCTGGAGA 
GACTCAAGTC GCCGGGACGA TAGGGATAGG GATGACCGTC GCCGTGAGAG GGATGACCGG 
CGTGATCTAA GAGAAAGACG AGATCTAAGA GACGACAGGG ACCGAAGAGG ACCTCCACTC 
AGATCAGAAC GTGAAGAAGT AAGTTCTTGG AGACGTGCTG ATGACAGGAA AGATGACCGG 
GTGGAAGAGC GGGACCCTCC TCGTCGAGTT CCTCCCCCAG CTCTTTCAAG AGACCGAGAA 
AGAGACCGAG ACCGAGAAAG AGAAGGTGAA AAAGAGAAGG CCTCATGGAG AGCTGAGAAA 
GATAGGGAAT CTCTCCGTCG TACTAAAAAT GAGACTGATG AAGATGGATG GACCACAGTA 
CGACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003741.1
CDS	209..4357
Translation	MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRIKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDSSLSRKDSRWGDRDSEGTWRKGPEADSEWRRGPPEKEWRRGEGRDEDRSHRRDEERPRRLGDDEDREPSLRPDDDRVPRRGMDDDRGPRRGPEEDRFSRRGADDDRPSWRNTDDDRPPRRIADEDRGNWRHADDDRPPRRGLDEDRGSWRTADEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPWRNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPRRGDDSRPGPWRPLVKPGGWREKEKAREESWGPPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWRDSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRVPPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR

Target ORF information:

RefSeq Version	NM_003750.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 3 subunit A (EIF3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003750.3

ORF Insert Sequence:

ATGCCGGCCT ATTTTCAGAG GCCGGAAAAT GCCCTCAAAC GCGCCAACGA ATTTCTTGAG 
GTTGGCAAAA AGCAGCCTGC TCTGGATGTT CTTTATGATG TTATGAAAAG TAAAAAACAT 
AGAACATGGC AAAAGATACA CGAACCAATT ATGTTGAAAT ACTTGGAACT TTGCGTGGAT 
CTTCGCAAGA GCCACTTGGC AAAGGAGGGG TTATACCAGT ATAAGAACAT TTGTCAACAG 
GTGAACATAA AATCTCTGGA GGATGTTGTT AGGGCATATT TGAAAATGGC AGAGGAAAAA 
ACTGAAGCTG CTAAAGAAGA ATCTCAGCAG ATGGTCTTAG ATATAGAGGA TCTAGATAAT 
ATTCAAACTC CTGAGAGTGT TCTCCTAAGT GCTGTAAGTG GTGAAGACAC TCAGGATCGT 
ACTGACAGAT TACTTTTAAC TCCATGGGTT AAATTCCTGT GGGAGTCTTA CAGGCAGTGT 
TTGGACCTTC TTAGAAACAA TTCTAGAGTA GAGCGCCTGT ACCATGATAT TGCCCAGCAA 
GCTTTCAAAT TCTGCCTCCA ATACACGCGT AAGGCTGAAT TCCGTAAACT GTGTGACAAT 
TTGAGAATGC ACTTATCGCA GATTCAGCGC CACCATAACC AAAGTACGGC AATCAATCTT 
AATAATCCAG AGAGCCAGTC CATGCATTTG GAAACCAGAC TTGTTCAGCT GGACAGTGCT 
ATCAGCATGG AATTGTGGCA GGAAGCATTC AAAGCTGTGG AAGATATTCA CGGGCTATTC 
TCCTTGTCTA AAAAACCACC TAAACCTCAG TTGATGGCAA ATTACTATAA CAAAGTCTCA 
ACTGTGTTTT GGAAATCTGG AAATGCTCTT TTTCATGCAT CTACACTCCA TCGTCTTTAC 
CATCTCTCTA GAGAAATGAG AAAGAATCTC ACACAAGATG AGATGCAAAG AATGTCTACT 
AGAGTCCTTT TAGCCACTCT TTCCATCCCT ATTACTCCTG AGCGTACGGA TATTGCTCGA 
CTTCTGGATA TGGATGGCAT TATAGTTGAA AAACAGCGTC GCCTTGCAAC ACTACTAGGT 
CTTCAAGCCC CACCGACACG AATTGGCCTT ATTAATGATA TGGTCAGATT TAATGTACTA 
CAATATGTTG TCCCAGAAGT GAAAGACCTT TACAATTGGC TTGAAGTAGA ATTTAACCCA 
TTAAAACTCT GTGAGCGAGT CACAAAGGTT CTAAATTGGG TTAGGGAACA ACCTGAAAAG 
GAACCGGAAT TGCAGCAGTA TGTGCCACAA CTGCAAAACA ACACCATCCT CCGCCTTCTG 
CAGCAGGTGT CACAGATTTA TCAGAGCATT GAGTTTTCTC GTTTGACTTC TTTGGTTCCT 
TTTGTTGATG CTTTCCAACT GGAACGGGCC ATAGTAGATG CAGCCAGGCA TTGCGACTTG 
CAGGTTCGTA TTGATCACAC TTCTCGGACC CTGAGTTTTG GATCTGATTT GAATTATGCT 
ACTCGAGAAG ATGCTCCGAT TGGTCCTCAT TTGCAAAGCA TGCCTTCAGA GCAGATAAGA 
AACCAGCTGA CAGCCATGTC CTCAGTACTT GCAAAAGCAC TTGAAGTCAT TAAACCAGCT 
CATATACTGC AAGAGAAAGA AGAACAGCAT CAGTTGGCTG TCACTGCATA CCTTAAAAAT 
TCACGAAAAG AGCACCAGCG GATCCTGGCT CGCCGCCAGA CAATTGAGGA GAGAAAAGAG 
CGCCTTGAGA GTCTGAATAT TCAGCGTGAG AAAGAAGAAT TGGAACAGAG GGAAGCTGAA 
CTCCAGAAAG TGCGGAAGGC TGAGGAAGAG AGGCTGCGCC AGGAAGCAAA GGAGAGAGAG 
AAGGAGCGTA TCTTACAGGA ACATGAACAA ATCAAAAAGA AAACTGTCCG AGAGCGTTTG 
GAGCAGATCA AGAAAACAGA ACTGGGTGCC AAAGCATTCA AAGATATTGA TATTGAAGAC 
CTTGAGGAAT TGGATCCAGA TTTTATCATG GCTAAACAGG TTGAACAACT GGAGAAAGAA 
AAGAAAGAAC TTCAAGAACG CCTAAAGAAT CAAGAAAAGA AGATTGACTA TTTTGAAAGA 
GCCAAACGTT TGGAAGAAAT TCCTTTGATA AAGAGCGCTT ACGAGGAACA GAGAATTAAA 
GACATGGATC TGTGGGAGCA ACAAGAGGAA GAAAGAATTA CTACAATGCA GCTAGAACGT 
GAAAAGGCTC TTGAACATAA GAATCGAATG TCACGAATGC TTGAAGACAG AGATTTATTC 
GTAATGCGAC TCAAAGCTGC ACGGCAGTCT GTTTATGAGG AAAAACTTAA ACAGTTTGAA 
GAGCGATTAG CAGAAGAAAG GCATAATCGA TTGGAAGAAC GGAAAAGGCA GCGTAAAGAA 
GAACGCAGGA TAACATACTA TAGAGAAAAA GAAGAGGAGG AGCAGAGAAG GGCAGAAGAA 
CAAATGCTAA AAGAGCGGGA AGAGAGAGAG CGCGCCGAAC GAGCAAAACG CGAGGAAGAG 
CTACGAGAGT ATCAGGAGCG GGTGAAGAAA TTAGAAGAAG TGGAAAGGAA AAAACGCCAA 
AGGGAGTTGG AAATTGAAGA ACGAGAACGG CGTAGAGAGG AAGAGAGAAG ACTTGGCGAT 
AGTTCCCTTT CTAGAAAGGA CTCTCGTTGG GGAGATAGAG ATTCAGAAGG CACCTGGAGA 
AAAGGACCTG AAGCAGATTC TGAGTGGAGA AGAGGCCCGC CAGAGAAGGA GTGGAGACGT 
GGAGAAGGGC GAGATGAGGA CAGGTCTCAT AGAAGAGATG AAGAGCGGCC CCGGCGTCTG 
GGGGATGATG AAGATAGAGA GCCCTCTCTT AGACCAGACG ATGATCGGGT TCCCCGGCGT 
GGCATGGATG ATGACAGAGG CCCTAGACGT GGTCCTGAGG AAGATAGGTT CTCTCGTCGT 
GGGGCAGACG ATGACCGGCC TTCCTGGCGT AACACAGATG ATGACAGGCC TCCCAGACGA 
ATTGCCGATG AAGACAGGGG AAACTGGCGT CATGCGGATG ATGACAGACC ACCTAGACGA 
GGACTGGATG AGGACAGAGG AAGCTGGCGA ACAGCTGATG AGGACAGAGG ACCAAGACGT 
GGGATGGATG ATGACCGGGG GCCGAGGCGA GGAGGCGCTG ATGATGAGCG ATCATCCTGG 
CGTAATGCTG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGCATGG ATGATGACCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG AGGACCTTGG 
AGGAACGCCG ATGATGACAG AATTCCCAGG CGTGGTGCAG AGGATGACAG GGGCCCTTGG 
AGAAACATGG ATGATGATCG CCTTTCAAGA CGTGCTGATG ATGATCGGTT TCCCAGACGG 
GGTGATGACT CAAGACCTGG TCCTTGGAGA CCATTAGTCA AGCCAGGTGG ATGGAGAGAG 
AAAGAAAAAG CCAGAGAGGA GAGCTGGGGT CCACCTCGAG AATCAAGGCC ATCAGAAGAA 
CGTGAATGGG ACAGAGAAAA AGAAAGGGAC AGAGATAATC AAGATCGGGA GGAGAATGAC 
AAGGACCCTG AGAGAGAAAG GGACAGAGAG AGAGATGTGG ATCGAGAGGA TCGCTTCAGA 
AGACCTAGGG ATGAAGGTGG CTGGAGAAGA GGACCAGCTG AGGAATCTTC AAGCTGGAGA 
GACTCAAGTC GCCGGGACGA TAGGGATAGG GATGACCGTC GCCGTGAGAG GGATGACCGG 
CGTGATCTAA GAGAAAGACG AGATCTAAGA GACGACAGGG ACCGAAGAGG ACCTCCACTC 
AGATCAGAAC GTGAAGAAGT AAGTTCTTGG AGACGTGCTG ATGACAGGAA AGATGACCGG 
GTGGAAGAGC GGGACCCTCC TCGTCGAGTT CCTCCCCCAG CTCTTTCAAG AGACCGAGAA 
AGAGACCGAG ACCGAGAAAG AGAAGGTGAA AAAGAGAAGG CCTCATGGAG AGCTGAGAAA 
GATAGGGAAT CTCTCCGTCG TACTAAAAAT GAGACTGATG AAGATGGATG GACCACAGTA 
CGACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF3A ( NM_003750.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003741.1 Homo sapiens eukaryotic translation initiation factor 3 subunit A (EIF3A), mRNA.

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CloneID	OHu17434
Clone ID Related Accession (Same CDS sequence)	NM_003750.2 , NM_003750.3 , NM_003750.4
Accession Version	NM_003750.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 3 subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AL355598.26 and AL157788.16. On Nov 22, 2018 this sequence version replaced NM_003750.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.10339.1, SRR1660807.136767.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369144.8/ ENSP00000358140.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGCCT ATTTTCAGAG GCCGGAAAAT GCCCTCAAAC GCGCCAACGA ATTTCTTGAG 
GTTGGCAAAA AGCAGCCTGC TCTGGATGTT CTTTATGATG TTATGAAAAG TAAAAAACAT 
AGAACATGGC AAAAGATACA CGAACCAATT ATGTTGAAAT ACTTGGAACT TTGCGTGGAT 
CTTCGCAAGA GCCACTTGGC AAAGGAGGGG TTATACCAGT ATAAGAACAT TTGTCAACAG 
GTGAACATAA AATCTCTGGA GGATGTTGTT AGGGCATATT TGAAAATGGC AGAGGAAAAA 
ACTGAAGCTG CTAAAGAAGA ATCTCAGCAG ATGGTCTTAG ATATAGAGGA TCTAGATAAT 
ATTCAAACTC CTGAGAGTGT TCTCCTAAGT GCTGTAAGTG GTGAAGACAC TCAGGATCGT 
ACTGACAGAT TACTTTTAAC TCCATGGGTT AAATTCCTGT GGGAGTCTTA CAGGCAGTGT 
TTGGACCTTC TTAGAAACAA TTCTAGAGTA GAGCGCCTGT ACCATGATAT TGCCCAGCAA 
GCTTTCAAAT TCTGCCTCCA ATACACGCGT AAGGCTGAAT TCCGTAAACT GTGTGACAAT 
TTGAGAATGC ACTTATCGCA GATTCAGCGC CACCATAACC AAAGTACGGC AATCAATCTT 
AATAATCCAG AGAGCCAGTC CATGCATTTG GAAACCAGAC TTGTTCAGCT GGACAGTGCT 
ATCAGCATGG AATTGTGGCA GGAAGCATTC AAAGCTGTGG AAGATATTCA CGGGCTATTC 
TCCTTGTCTA AAAAACCACC TAAACCTCAG TTGATGGCAA ATTACTATAA CAAAGTCTCA 
ACTGTGTTTT GGAAATCTGG AAATGCTCTT TTTCATGCAT CTACACTCCA TCGTCTTTAC 
CATCTCTCTA GAGAAATGAG AAAGAATCTC ACACAAGATG AGATGCAAAG AATGTCTACT 
AGAGTCCTTT TAGCCACTCT TTCCATCCCT ATTACTCCTG AGCGTACGGA TATTGCTCGA 
CTTCTGGATA TGGATGGCAT TATAGTTGAA AAACAGCGTC GCCTTGCAAC ACTACTAGGT 
CTTCAAGCCC CACCGACACG AATTGGCCTT ATTAATGATA TGGTCAGATT TAATGTACTA 
CAATATGTTG TCCCAGAAGT GAAAGACCTT TACAATTGGC TTGAAGTAGA ATTTAACCCA 
TTAAAACTCT GTGAGCGAGT CACAAAGGTT CTAAATTGGG TTAGGGAACA ACCTGAAAAG 
GAACCGGAAT TGCAGCAGTA TGTGCCACAA CTGCAAAACA ACACCATCCT CCGCCTTCTG 
CAGCAGGTGT CACAGATTTA TCAGAGCATT GAGTTTTCTC GTTTGACTTC TTTGGTTCCT 
TTTGTTGATG CTTTCCAACT GGAACGGGCC ATAGTAGATG CAGCCAGGCA TTGCGACTTG 
CAGGTTCGTA TTGATCACAC TTCTCGGACC CTGAGTTTTG GATCTGATTT GAATTATGCT 
ACTCGAGAAG ATGCTCCGAT TGGTCCTCAT TTGCAAAGCA TGCCTTCAGA GCAGATAAGA 
AACCAGCTGA CAGCCATGTC CTCAGTACTT GCAAAAGCAC TTGAAGTCAT TAAACCAGCT 
CATATACTGC AAGAGAAAGA AGAACAGCAT CAGTTGGCTG TCACTGCATA CCTTAAAAAT 
TCACGAAAAG AGCACCAGCG GATCCTGGCT CGCCGCCAGA CAATTGAGGA GAGAAAAGAG 
CGCCTTGAGA GTCTGAATAT TCAGCGTGAG AAAGAAGAAT TGGAACAGAG GGAAGCTGAA 
CTCCAGAAAG TGCGGAAGGC TGAGGAAGAG AGGCTGCGCC AGGAAGCAAA GGAGAGAGAG 
AAGGAGCGTA TCTTACAGGA ACATGAACAA ATCAAAAAGA AAACTGTCCG AGAGCGTTTG 
GAGCAGATCA AGAAAACAGA ACTGGGTGCC AAAGCATTCA AAGATATTGA TATTGAAGAC 
CTTGAGGAAT TGGATCCAGA TTTTATCATG GCTAAACAGG TTGAACAACT GGAGAAAGAA 
AAGAAAGAAC TTCAAGAACG CCTAAAGAAT CAAGAAAAGA AGATTGACTA TTTTGAAAGA 
GCCAAACGTT TGGAAGAAAT TCCTTTGATA AAGAGCGCTT ACGAGGAACA GAGAATTAAA 
GACATGGATC TGTGGGAGCA ACAAGAGGAA GAAAGAATTA CTACAATGCA GCTAGAACGT 
GAAAAGGCTC TTGAACATAA GAATCGAATG TCACGAATGC TTGAAGACAG AGATTTATTC 
GTAATGCGAC TCAAAGCTGC ACGGCAGTCT GTTTATGAGG AAAAACTTAA ACAGTTTGAA 
GAGCGATTAG CAGAAGAAAG GCATAATCGA TTGGAAGAAC GGAAAAGGCA GCGTAAAGAA 
GAACGCAGGA TAACATACTA TAGAGAAAAA GAAGAGGAGG AGCAGAGAAG GGCAGAAGAA 
CAAATGCTAA AAGAGCGGGA AGAGAGAGAG CGCGCCGAAC GAGCAAAACG CGAGGAAGAG 
CTACGAGAGT ATCAGGAGCG GGTGAAGAAA TTAGAAGAAG TGGAAAGGAA AAAACGCCAA 
AGGGAGTTGG AAATTGAAGA ACGAGAACGG CGTAGAGAGG AAGAGAGAAG ACTTGGCGAT 
AGTTCCCTTT CTAGAAAGGA CTCTCGTTGG GGAGATAGAG ATTCAGAAGG CACCTGGAGA 
AAAGGACCTG AAGCAGATTC TGAGTGGAGA AGAGGCCCGC CAGAGAAGGA GTGGAGACGT 
GGAGAAGGGC GAGATGAGGA CAGGTCTCAT AGAAGAGATG AAGAGCGGCC CCGGCGTCTG 
GGGGATGATG AAGATAGAGA GCCCTCTCTT AGACCAGACG ATGATCGGGT TCCCCGGCGT 
GGCATGGATG ATGACAGAGG CCCTAGACGT GGTCCTGAGG AAGATAGGTT CTCTCGTCGT 
GGGGCAGACG ATGACCGGCC TTCCTGGCGT AACACAGATG ATGACAGGCC TCCCAGACGA 
ATTGCCGATG AAGACAGGGG AAACTGGCGT CATGCGGATG ATGACAGACC ACCTAGACGA 
GGACTGGATG AGGACAGAGG AAGCTGGCGA ACAGCTGATG AGGACAGAGG ACCAAGACGT 
GGGATGGATG ATGACCGGGG GCCGAGGCGA GGAGGCGCTG ATGATGAGCG ATCATCCTGG 
CGTAATGCTG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGCATGG ATGATGACCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG AGGACCTTGG 
AGGAACGCCG ATGATGACAG AATTCCCAGG CGTGGTGCAG AGGATGACAG GGGCCCTTGG 
AGAAACATGG ATGATGATCG CCTTTCAAGA CGTGCTGATG ATGATCGGTT TCCCAGACGG 
GGTGATGACT CAAGACCTGG TCCTTGGAGA CCATTAGTCA AGCCAGGTGG ATGGAGAGAG 
AAAGAAAAAG CCAGAGAGGA GAGCTGGGGT CCACCTCGAG AATCAAGGCC ATCAGAAGAA 
CGTGAATGGG ACAGAGAAAA AGAAAGGGAC AGAGATAATC AAGATCGGGA GGAGAATGAC 
AAGGACCCTG AGAGAGAAAG GGACAGAGAG AGAGATGTGG ATCGAGAGGA TCGCTTCAGA 
AGACCTAGGG ATGAAGGTGG CTGGAGAAGA GGACCAGCTG AGGAATCTTC AAGCTGGAGA 
GACTCAAGTC GCCGGGACGA TAGGGATAGG GATGACCGTC GCCGTGAGAG GGATGACCGG 
CGTGATCTAA GAGAAAGACG AGATCTAAGA GACGACAGGG ACCGAAGAGG ACCTCCACTC 
AGATCAGAAC GTGAAGAAGT AAGTTCTTGG AGACGTGCTG ATGACAGGAA AGATGACCGG 
GTGGAAGAGC GGGACCCTCC TCGTCGAGTT CCTCCCCCAG CTCTTTCAAG AGACCGAGAA 
AGAGACCGAG ACCGAGAAAG AGAAGGTGAA AAAGAGAAGG CCTCATGGAG AGCTGAGAAA 
GATAGGGAAT CTCTCCGTCG TACTAAAAAT GAGACTGATG AAGATGGATG GACCACAGTA 
CGACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003741.1
CDS	142..4290
Translation	MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRIKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDSSLSRKDSRWGDRDSEGTWRKGPEADSEWRRGPPEKEWRRGEGRDEDRSHRRDEERPRRLGDDEDREPSLRPDDDRVPRRGMDDDRGPRRGPEEDRFSRRGADDDRPSWRNTDDDRPPRRIADEDRGNWRHADDDRPPRRGLDEDRGSWRTADEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPWRNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPRRGDDSRPGPWRPLVKPGGWREKEKAREESWGPPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWRDSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRVPPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR

Target ORF information:

RefSeq Version	NM_003750.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 3 subunit A (EIF3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003750.4

ORF Insert Sequence:

ATGCCGGCCT ATTTTCAGAG GCCGGAAAAT GCCCTCAAAC GCGCCAACGA ATTTCTTGAG 
GTTGGCAAAA AGCAGCCTGC TCTGGATGTT CTTTATGATG TTATGAAAAG TAAAAAACAT 
AGAACATGGC AAAAGATACA CGAACCAATT ATGTTGAAAT ACTTGGAACT TTGCGTGGAT 
CTTCGCAAGA GCCACTTGGC AAAGGAGGGG TTATACCAGT ATAAGAACAT TTGTCAACAG 
GTGAACATAA AATCTCTGGA GGATGTTGTT AGGGCATATT TGAAAATGGC AGAGGAAAAA 
ACTGAAGCTG CTAAAGAAGA ATCTCAGCAG ATGGTCTTAG ATATAGAGGA TCTAGATAAT 
ATTCAAACTC CTGAGAGTGT TCTCCTAAGT GCTGTAAGTG GTGAAGACAC TCAGGATCGT 
ACTGACAGAT TACTTTTAAC TCCATGGGTT AAATTCCTGT GGGAGTCTTA CAGGCAGTGT 
TTGGACCTTC TTAGAAACAA TTCTAGAGTA GAGCGCCTGT ACCATGATAT TGCCCAGCAA 
GCTTTCAAAT TCTGCCTCCA ATACACGCGT AAGGCTGAAT TCCGTAAACT GTGTGACAAT 
TTGAGAATGC ACTTATCGCA GATTCAGCGC CACCATAACC AAAGTACGGC AATCAATCTT 
AATAATCCAG AGAGCCAGTC CATGCATTTG GAAACCAGAC TTGTTCAGCT GGACAGTGCT 
ATCAGCATGG AATTGTGGCA GGAAGCATTC AAAGCTGTGG AAGATATTCA CGGGCTATTC 
TCCTTGTCTA AAAAACCACC TAAACCTCAG TTGATGGCAA ATTACTATAA CAAAGTCTCA 
ACTGTGTTTT GGAAATCTGG AAATGCTCTT TTTCATGCAT CTACACTCCA TCGTCTTTAC 
CATCTCTCTA GAGAAATGAG AAAGAATCTC ACACAAGATG AGATGCAAAG AATGTCTACT 
AGAGTCCTTT TAGCCACTCT TTCCATCCCT ATTACTCCTG AGCGTACGGA TATTGCTCGA 
CTTCTGGATA TGGATGGCAT TATAGTTGAA AAACAGCGTC GCCTTGCAAC ACTACTAGGT 
CTTCAAGCCC CACCGACACG AATTGGCCTT ATTAATGATA TGGTCAGATT TAATGTACTA 
CAATATGTTG TCCCAGAAGT GAAAGACCTT TACAATTGGC TTGAAGTAGA ATTTAACCCA 
TTAAAACTCT GTGAGCGAGT CACAAAGGTT CTAAATTGGG TTAGGGAACA ACCTGAAAAG 
GAACCGGAAT TGCAGCAGTA TGTGCCACAA CTGCAAAACA ACACCATCCT CCGCCTTCTG 
CAGCAGGTGT CACAGATTTA TCAGAGCATT GAGTTTTCTC GTTTGACTTC TTTGGTTCCT 
TTTGTTGATG CTTTCCAACT GGAACGGGCC ATAGTAGATG CAGCCAGGCA TTGCGACTTG 
CAGGTTCGTA TTGATCACAC TTCTCGGACC CTGAGTTTTG GATCTGATTT GAATTATGCT 
ACTCGAGAAG ATGCTCCGAT TGGTCCTCAT TTGCAAAGCA TGCCTTCAGA GCAGATAAGA 
AACCAGCTGA CAGCCATGTC CTCAGTACTT GCAAAAGCAC TTGAAGTCAT TAAACCAGCT 
CATATACTGC AAGAGAAAGA AGAACAGCAT CAGTTGGCTG TCACTGCATA CCTTAAAAAT 
TCACGAAAAG AGCACCAGCG GATCCTGGCT CGCCGCCAGA CAATTGAGGA GAGAAAAGAG 
CGCCTTGAGA GTCTGAATAT TCAGCGTGAG AAAGAAGAAT TGGAACAGAG GGAAGCTGAA 
CTCCAGAAAG TGCGGAAGGC TGAGGAAGAG AGGCTGCGCC AGGAAGCAAA GGAGAGAGAG 
AAGGAGCGTA TCTTACAGGA ACATGAACAA ATCAAAAAGA AAACTGTCCG AGAGCGTTTG 
GAGCAGATCA AGAAAACAGA ACTGGGTGCC AAAGCATTCA AAGATATTGA TATTGAAGAC 
CTTGAGGAAT TGGATCCAGA TTTTATCATG GCTAAACAGG TTGAACAACT GGAGAAAGAA 
AAGAAAGAAC TTCAAGAACG CCTAAAGAAT CAAGAAAAGA AGATTGACTA TTTTGAAAGA 
GCCAAACGTT TGGAAGAAAT TCCTTTGATA AAGAGCGCTT ACGAGGAACA GAGAATTAAA 
GACATGGATC TGTGGGAGCA ACAAGAGGAA GAAAGAATTA CTACAATGCA GCTAGAACGT 
GAAAAGGCTC TTGAACATAA GAATCGAATG TCACGAATGC TTGAAGACAG AGATTTATTC 
GTAATGCGAC TCAAAGCTGC ACGGCAGTCT GTTTATGAGG AAAAACTTAA ACAGTTTGAA 
GAGCGATTAG CAGAAGAAAG GCATAATCGA TTGGAAGAAC GGAAAAGGCA GCGTAAAGAA 
GAACGCAGGA TAACATACTA TAGAGAAAAA GAAGAGGAGG AGCAGAGAAG GGCAGAAGAA 
CAAATGCTAA AAGAGCGGGA AGAGAGAGAG CGCGCCGAAC GAGCAAAACG CGAGGAAGAG 
CTACGAGAGT ATCAGGAGCG GGTGAAGAAA TTAGAAGAAG TGGAAAGGAA AAAACGCCAA 
AGGGAGTTGG AAATTGAAGA ACGAGAACGG CGTAGAGAGG AAGAGAGAAG ACTTGGCGAT 
AGTTCCCTTT CTAGAAAGGA CTCTCGTTGG GGAGATAGAG ATTCAGAAGG CACCTGGAGA 
AAAGGACCTG AAGCAGATTC TGAGTGGAGA AGAGGCCCGC CAGAGAAGGA GTGGAGACGT 
GGAGAAGGGC GAGATGAGGA CAGGTCTCAT AGAAGAGATG AAGAGCGGCC CCGGCGTCTG 
GGGGATGATG AAGATAGAGA GCCCTCTCTT AGACCAGACG ATGATCGGGT TCCCCGGCGT 
GGCATGGATG ATGACAGAGG CCCTAGACGT GGTCCTGAGG AAGATAGGTT CTCTCGTCGT 
GGGGCAGACG ATGACCGGCC TTCCTGGCGT AACACAGATG ATGACAGGCC TCCCAGACGA 
ATTGCCGATG AAGACAGGGG AAACTGGCGT CATGCGGATG ATGACAGACC ACCTAGACGA 
GGACTGGATG AGGACAGAGG AAGCTGGCGA ACAGCTGATG AGGACAGAGG ACCAAGACGT 
GGGATGGATG ATGACCGGGG GCCGAGGCGA GGAGGCGCTG ATGATGAGCG ATCATCCTGG 
CGTAATGCTG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGCATGG ATGATGACCG GGGTCCCAGG 
CGAGGCATGG ATGATGACCG GGGTCCCAGG CGAGGGTTGG ATGATGATCG AGGACCTTGG 
AGGAACGCCG ATGATGACAG AATTCCCAGG CGTGGTGCAG AGGATGACAG GGGCCCTTGG 
AGAAACATGG ATGATGATCG CCTTTCAAGA CGTGCTGATG ATGATCGGTT TCCCAGACGG 
GGTGATGACT CAAGACCTGG TCCTTGGAGA CCATTAGTCA AGCCAGGTGG ATGGAGAGAG 
AAAGAAAAAG CCAGAGAGGA GAGCTGGGGT CCACCTCGAG AATCAAGGCC ATCAGAAGAA 
CGTGAATGGG ACAGAGAAAA AGAAAGGGAC AGAGATAATC AAGATCGGGA GGAGAATGAC 
AAGGACCCTG AGAGAGAAAG GGACAGAGAG AGAGATGTGG ATCGAGAGGA TCGCTTCAGA 
AGACCTAGGG ATGAAGGTGG CTGGAGAAGA GGACCAGCTG AGGAATCTTC AAGCTGGAGA 
GACTCAAGTC GCCGGGACGA TAGGGATAGG GATGACCGTC GCCGTGAGAG GGATGACCGG 
CGTGATCTAA GAGAAAGACG AGATCTAAGA GACGACAGGG ACCGAAGAGG ACCTCCACTC 
AGATCAGAAC GTGAAGAAGT AAGTTCTTGG AGACGTGCTG ATGACAGGAA AGATGACCGG 
GTGGAAGAGC GGGACCCTCC TCGTCGAGTT CCTCCCCCAG CTCTTTCAAG AGACCGAGAA 
AGAGACCGAG ACCGAGAAAG AGAAGGTGAA AAAGAGAAGG CCTCATGGAG AGCTGAGAAA 
GATAGGGAAT CTCTCCGTCG TACTAAAAAT GAGACTGATG AAGATGGATG GACCACAGTA 
CGACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knockdown of elF3a inhibits TGF??1?induced extracellular matrix protein expression in keloid fibroblasts.
Molecular medicine reports(2017 Dec)
Li T,Zhao J

Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle.
Nucleic acids research45(19)10948-10968(2017 Nov)
Val??ek LS,Zeman J,Wagner S,Beznoskov? P,Pavl?kov? Z,Mohammad MP,Hronov? V,Herrmannov? A,Hashem Y,Guni?ov? S

The eIF3a Arg803Lys genetic polymorphism is associated with susceptibility to and chemoradiotherapy efficacy in cervical carcinoma.
The Kaohsiung journal of medical sciences33(4)187-194(2017 Apr)
Xu JZ,Wen F,Wang XR

MiR-488 inhibits proliferation and cisplatin sensibility in non-small-cell lung cancer (NSCLC) cells by activating the eIF3a-mediated NER signaling pathway.
Scientific reports740384(2017 Jan)
Fang C,Chen YX,Wu NY,Yin JY,Li XP,Huang HS,Zhang W,Zhou HH,Liu ZQ

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EIF3A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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