AKR1C3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AKR1C3
Entrez Gene ID	8644
Full Name	aldo-keto reductase family 1 member C3
Synonyms	DD3,DDX,HA1753,HAKRB,HAKRe,HSD17B5,PGFS,hluPGFS
General protein information	Preferred Names aldo-keto reductase family 1 member C3 Names aldo-keto reductase family 1 member C3 3-alpha hydroxysteroid dehydrogenase, type II 3-alpha-HSD type II, brain chlordecone reductase homolog HAKRb dihydrodiol dehydrogenase 3 dihydrodiol dehydrogenase X indanol dehydrogenase prostaglandin F synthase testosterone 17-beta-dehydrogenase 5 trans-1,2-dihydrobenzene-1,2-diol dehydrogenase type IIb 3-alpha hydroxysteroid dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p15.1
Summary	This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011].
Disorder MIM:	603966

mRNA and Protein(s)

mRNA	Protein	Name
NM_001253909.2	NP_001240838.1	aldo-keto reductase family 1 member C3 isoform 3
NM_001253908.1	NP_001240837.1	aldo-keto reductase family 1 member C3 isoform 2
NM_003739.6	NP_003730.4	aldo-keto reductase family 1 member C3 isoform 1
NM_003739.5	NP_003730.4	aldo-keto reductase family 1 member C3 isoform 1
NM_001253909.1	NP_001240838.1	aldo-keto reductase family 1 member C3 isoform 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AKR1C3	NP_001012344.1
Bos taurus (cattle)	AKR1C3	XP_002692230.1
Bos taurus (cattle)	LOC100337056	XP_002692229.1
Bos taurus (cattle)	LOC782922	NP_001159696.1
Bos taurus (cattle)	LOC527068	NP_001030444.1
Mus musculus (house mouse)	Akr1c18	NP_598827.1
Bos taurus (cattle)	LOC784927	XP_005214488.1
Rattus norvegicus (Norway rat)	Akr1c3	NP_612519.1
Pan troglodytes (chimpanzee)	AKR1C3	XP_003951631.1
Bos taurus (cattle)	AKR1C4	NP_851370.1
Bos taurus (cattle)	20ALPHA-HSD	NP_001161132.1
Homo sapiens (human)	AKR1C3	NP_001240837.1
Bos taurus (cattle)	LOC506594	XP_002692251.3
Bos taurus (cattle)	LOC782061	NP_001159695.1
Bos taurus (cattle)	LOC785762	NP_001159697.1
Bos taurus (cattle)	LOC538060	NP_001193716.1

Gene Ontology
Bibliography

Related articles in PubMed

17beta-hydroxysteroid dehydrogenase type 5 is negatively correlated to apoptosis inhibitor GRP78 and tumor-secreted protein PGK1, and modulates breast cancer cell viability and proliferation.
Xu D, Aka JA, Wang R, Lin SX
The Journal of steroid biochemistry and molecular biology171270-280(2017 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The replacement of C154 with a residue possessing a bulky aromatic side-chain impairs the folding of the alpha-helix containing C154 and its neighboring secondary structures, leading to low thermostability of AKR1C3.
Title: Instability of C154Y variant of aldo-keto reductase 1C3.

Data suggest that, in breast cancer cells, expression of HSD17B5 and expression of GRP78 (an apoptosis inhibitor) are strongly but negatively correlated; GRP78 knockdown decreases breast cancer cell viability whereas HSD17B5 knockdown increases cell viability and cell proliferation. (HSD17B5, 17-beta-hydroxysteroid dehydrogenase 5; GRP78, 78 kDa glucose-regulated protein)
Title: 17beta-hydroxysteroid dehydrogenase type 5 is negatively correlated to apoptosis inhibitor GRP78 and tumor-secreted protein PGK1, and modulates breast cancer cell viability and proliferation.

the present study suggests that AKR1C1, AKR1C2, AKR1C3, and AKR1C4 are closely associated with drug resistance to both CDDP and 5FU, and that mefenamic acid, an inhibitor of AKR1C, restores sensitivity through inhibition of drug-resistance in human cancer cells.
Title: Mefenamic acid enhances anticancer drug sensitivity via inhibition of aldo-keto reductase 1C enzyme activity.

Five common AKR1C3 polymorphisms were associated with decreased rates of exemestane catalysis.
Title: Impact of nonsynonymous single nucleotide polymorphisms on in-vitro metabolism of exemestane by hepatic cytosolic reductases.

If our these findings can be reproduced in larger homogeneous cohorts, a genetic stratification based on the AKR1C3 rs12529 single nucleotide polymorphism, can minimize androgen deprivation therapy-related health-related quality of life effects in prostate cancer patients
Title: Quality of life effects of androgen deprivation therapy in a prostate cancer cohort in New Zealand: can we minimize effects using a stratification based on the aldo-keto reductase family 1, member C3 rs12529 gene polymorphism?

The following AKR1C3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKR1C3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15813	NM_003739.6 Latest version!	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15684	NM_001253908.1 Latest version!	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu15964	NM_001253909.2 Latest version!	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu15813	NM_003739.5	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15964	NM_001253909.1	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AKR1C3 ( NM_003739.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_003730.4 Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15813
Clone ID Related Accession (Same CDS sequence)	NM_003739.6 , NM_003739.5
Accession Version	NM_003739.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	aldo-keto reductase family 1 member C3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV761799.2 and BC019230.1. On Nov 22, 2018 this sequence version replaced NM_003739.5. Transcript Variant: This variant (1) represents the longest transcript and encodes one of the larger isoforms (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001479.2, AB018580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000380554.5/ ENSP00000369927.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATTCCA AACACCAGTG TGTAAAGCTA AATGATGGCC ACTTCATGCC TGTATTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGC CAGGTGAGGA ACTTTCACCA ACAGATGAAA ATGGAAAAGT AATATTTGAC 
ATAGTGGATC TCTGTACCAC CTGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGATTGGCC 
AAGTCCATTG GGGTGTCAAA CTTCAACCGC AGGCAGCTGG AGATGATCCT CAACAAGCCA 
GGACTCAAGT ACAAGCCTGT CTGCAACCAG GTAGAATGTC ATCCGTATTT CAACCGGAGT 
AAATTGCTAG ATTTCTGCAA GTCGAAAGAT ATTGTTCTGG TTGCCTATAG TGCTCTGGGA 
TCTCAACGAG ACAAACGATG GGTGGACCCG AACTCCCCGG TGCTCTTGGA GGACCCAGTC 
CTTTGTGCCT TGGCAAAAAA GCACAAGCGA ACCCCAGCCC TGATTGCCCT GCGCTACCAG 
CTGCAGCGTG GGGTTGTGGT CCTGGCCAAG AGCTACAATG AGCAGCGCAT CAGACAGAAC 
GTGCAGGTTT TTGAGTTCCA GTTGACTGCA GAGGACATGA AAGCCATAGA TGGCCTAGAC 
AGAAATCTCC ACTATTTTAA CAGTGATAGT TTTGCTAGCC ACCCTAATTA TCCATATTCA 
GATGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003730.4
CDS	32..1003
Translation	MDSKHQCVKLNDGHFMPVLGFGTYAPPEVPRSKALEVTKLAIEAGFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSTFHRPELVRPALENSLKKAQLDYVDLYLIHSPMSLKPGEELSPTDENGKVIFDIVDLCTTWEAMEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNRSKLLDFCKSKDIVLVAYSALGSQRDKRWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTAEDMKAIDGLDRNLHYFNSDSFASHPNYPYSDEY

Target ORF information:

RefSeq Version	NM_003739.6
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003739.6

ORF Insert Sequence:

ATGGATTCCA AACACCAGTG TGTAAAGCTA AATGATGGCC ACTTCATGCC TGTATTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGC CAGGTGAGGA ACTTTCACCA ACAGATGAAA ATGGAAAAGT AATATTTGAC 
ATAGTGGATC TCTGTACCAC CTGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGATTGGCC 
AAGTCCATTG GGGTGTCAAA CTTCAACCGC AGGCAGCTGG AGATGATCCT CAACAAGCCA 
GGACTCAAGT ACAAGCCTGT CTGCAACCAG GTAGAATGTC ATCCGTATTT CAACCGGAGT 
AAATTGCTAG ATTTCTGCAA GTCGAAAGAT ATTGTTCTGG TTGCCTATAG TGCTCTGGGA 
TCTCAACGAG ACAAACGATG GGTGGACCCG AACTCCCCGG TGCTCTTGGA GGACCCAGTC 
CTTTGTGCCT TGGCAAAAAA GCACAAGCGA ACCCCAGCCC TGATTGCCCT GCGCTACCAG 
CTGCAGCGTG GGGTTGTGGT CCTGGCCAAG AGCTACAATG AGCAGCGCAT CAGACAGAAC 
GTGCAGGTTT TTGAGTTCCA GTTGACTGCA GAGGACATGA AAGCCATAGA TGGCCTAGAC 
AGAAATCTCC ACTATTTTAA CAGTGATAGT TTTGCTAGCC ACCCTAATTA TCCATATTCA 
GATGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKR1C3 ( NM_003739.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003730.4 Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15813
Clone ID Related Accession (Same CDS sequence)	NM_003739.6 , NM_003739.5
Accession Version	NM_003739.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	aldo-keto reductase family 1 member C3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV761799.2 and BC019230.1. On Dec 10, 2011 this sequence version replaced NM_003739.4. Transcript Variant: This variant (1) represents the longest transcript and encodes one of the larger isoforms (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001479.2, D17793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCA AACACCAGTG TGTAAAGCTA AATGATGGCC ACTTCATGCC TGTATTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGC CAGGTGAGGA ACTTTCACCA ACAGATGAAA ATGGAAAAGT AATATTTGAC 
ATAGTGGATC TCTGTACCAC CTGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGATTGGCC 
AAGTCCATTG GGGTGTCAAA CTTCAACCGC AGGCAGCTGG AGATGATCCT CAACAAGCCA 
GGACTCAAGT ACAAGCCTGT CTGCAACCAG GTAGAATGTC ATCCGTATTT CAACCGGAGT 
AAATTGCTAG ATTTCTGCAA GTCGAAAGAT ATTGTTCTGG TTGCCTATAG TGCTCTGGGA 
TCTCAACGAG ACAAACGATG GGTGGACCCG AACTCCCCGG TGCTCTTGGA GGACCCAGTC 
CTTTGTGCCT TGGCAAAAAA GCACAAGCGA ACCCCAGCCC TGATTGCCCT GCGCTACCAG 
CTGCAGCGTG GGGTTGTGGT CCTGGCCAAG AGCTACAATG AGCAGCGCAT CAGACAGAAC 
GTGCAGGTTT TTGAGTTCCA GTTGACTGCA GAGGACATGA AAGCCATAGA TGGCCTAGAC 
AGAAATCTCC ACTATTTTAA CAGTGATAGT TTTGCTAGCC ACCCTAATTA TCCATATTCA 
GATGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003730.4
CDS	70..1041
Translation	MDSKHQCVKLNDGHFMPVLGFGTYAPPEVPRSKALEVTKLAIEAGFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSTFHRPELVRPALENSLKKAQLDYVDLYLIHSPMSLKPGEELSPTDENGKVIFDIVDLCTTWEAMEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNRSKLLDFCKSKDIVLVAYSALGSQRDKRWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTAEDMKAIDGLDRNLHYFNSDSFASHPNYPYSDEY

Target ORF information:

RefSeq Version	NM_003739.5
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003739.5

ORF Insert Sequence:

ATGGATTCCA AACACCAGTG TGTAAAGCTA AATGATGGCC ACTTCATGCC TGTATTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGC CAGGTGAGGA ACTTTCACCA ACAGATGAAA ATGGAAAAGT AATATTTGAC 
ATAGTGGATC TCTGTACCAC CTGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGATTGGCC 
AAGTCCATTG GGGTGTCAAA CTTCAACCGC AGGCAGCTGG AGATGATCCT CAACAAGCCA 
GGACTCAAGT ACAAGCCTGT CTGCAACCAG GTAGAATGTC ATCCGTATTT CAACCGGAGT 
AAATTGCTAG ATTTCTGCAA GTCGAAAGAT ATTGTTCTGG TTGCCTATAG TGCTCTGGGA 
TCTCAACGAG ACAAACGATG GGTGGACCCG AACTCCCCGG TGCTCTTGGA GGACCCAGTC 
CTTTGTGCCT TGGCAAAAAA GCACAAGCGA ACCCCAGCCC TGATTGCCCT GCGCTACCAG 
CTGCAGCGTG GGGTTGTGGT CCTGGCCAAG AGCTACAATG AGCAGCGCAT CAGACAGAAC 
GTGCAGGTTT TTGAGTTCCA GTTGACTGCA GAGGACATGA AAGCCATAGA TGGCCTAGAC 
AGAAATCTCC ACTATTTTAA CAGTGATAGT TTTGCTAGCC ACCCTAATTA TCCATATTCA 
GATGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKR1C3 ( NM_001253908.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001240837.1 Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15684
Clone ID Related Accession (Same CDS sequence)	NM_001253908.1
Accession Version	NM_001253908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	aldo-keto reductase family 1 member C3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from H79159.1, BG547635.1 and BC019230.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is the same length as isoform 1 but differs by 1 aa in the N-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ269985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCAA AACATCAGTG TTTGAAGCTA AATGATGGTC ACTTCATGCC TGTCCTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGC CAGGTGAGGA ACTTTCACCA ACAGATGAAA ATGGAAAAGT AATATTTGAC 
ATAGTGGATC TCTGTACCAC CTGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGATTGGCC 
AAGTCCATTG GGGTGTCAAA CTTCAACCGC AGGCAGCTGG AGATGATCCT CAACAAGCCA 
GGACTCAAGT ACAAGCCTGT CTGCAACCAG GTAGAATGTC ATCCGTATTT CAACCGGAGT 
AAATTGCTAG ATTTCTGCAA GTCGAAAGAT ATTGTTCTGG TTGCCTATAG TGCTCTGGGA 
TCTCAACGAG ACAAACGATG GGTGGACCCG AACTCCCCGG TGCTCTTGGA GGACCCAGTC 
CTTTGTGCCT TGGCAAAAAA GCACAAGCGA ACCCCAGCCC TGATTGCCCT GCGCTACCAG 
CTGCAGCGTG GGGTTGTGGT CCTGGCCAAG AGCTACAATG AGCAGCGCAT CAGACAGAAC 
GTGCAGGTTT TTGAGTTCCA GTTGACTGCA GAGGACATGA AAGCCATAGA TGGCCTAGAC 
AGAAATCTCC ACTATTTTAA CAGTGATAGT TTTGCTAGCC ACCCTAATTA TCCATATTCA 
GATGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240837.1
CDS	47..1018
Translation	MDSKHQCLKLNDGHFMPVLGFGTYAPPEVPRSKALEVTKLAIEAGFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSTFHRPELVRPALENSLKKAQLDYVDLYLIHSPMSLKPGEELSPTDENGKVIFDIVDLCTTWEAMEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNRSKLLDFCKSKDIVLVAYSALGSQRDKRWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTAEDMKAIDGLDRNLHYFNSDSFASHPNYPYSDEY

Target ORF information:

RefSeq Version	NM_001253908.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253908.1

ORF Insert Sequence:

ATGGATTCAA AACATCAGTG TTTGAAGCTA AATGATGGTC ACTTCATGCC TGTCCTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGC CAGGTGAGGA ACTTTCACCA ACAGATGAAA ATGGAAAAGT AATATTTGAC 
ATAGTGGATC TCTGTACCAC CTGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGATTGGCC 
AAGTCCATTG GGGTGTCAAA CTTCAACCGC AGGCAGCTGG AGATGATCCT CAACAAGCCA 
GGACTCAAGT ACAAGCCTGT CTGCAACCAG GTAGAATGTC ATCCGTATTT CAACCGGAGT 
AAATTGCTAG ATTTCTGCAA GTCGAAAGAT ATTGTTCTGG TTGCCTATAG TGCTCTGGGA 
TCTCAACGAG ACAAACGATG GGTGGACCCG AACTCCCCGG TGCTCTTGGA GGACCCAGTC 
CTTTGTGCCT TGGCAAAAAA GCACAAGCGA ACCCCAGCCC TGATTGCCCT GCGCTACCAG 
CTGCAGCGTG GGGTTGTGGT CCTGGCCAAG AGCTACAATG AGCAGCGCAT CAGACAGAAC 
GTGCAGGTTT TTGAGTTCCA GTTGACTGCA GAGGACATGA AAGCCATAGA TGGCCTAGAC 
AGAAATCTCC ACTATTTTAA CAGTGATAGT TTTGCTAGCC ACCCTAATTA TCCATATTCA 
GATGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKR1C3 ( NM_001253909.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001240838.1 Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu15964
Clone ID Related Accession (Same CDS sequence)	NM_001253909.2 , NM_001253909.1
Accession Version	NM_001253909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	aldo-keto reductase family 1 member C3 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296701.1 and AL391427.10. On May 31, 2019 this sequence version replaced NM_001253909.1. Transcript Variant: This variant (3) lacks several exons at its 3' end, which extends into an intron compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296701.1, DC345588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGATTCCA AACACCAGTG TGTAAAGCTA AATGATGGCC ACTTCATGCC TGTATTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGG TATGCAGTTT GTATGAGCAT AAAATTGCGC TTCTGCTGTC ATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240838.1
CDS	32..448
Translation	MDSKHQCVKLNDGHFMPVLGFGTYAPPEVPRSKALEVTKLAIEAGFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSTFHRPELVRPALENSLKKAQLDYVDLYLIHSPMSLKVCSLYEHKIALLLSL

Target ORF information:

RefSeq Version	NM_001253909.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253909.2

ORF Insert Sequence:

ATGGATTCCA AACACCAGTG TGTAAAGCTA AATGATGGCC ACTTCATGCC TGTATTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGG TATGCAGTTT GTATGAGCAT AAAATTGCGC TTCTGCTGTC ATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKR1C3 ( NM_001253909.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001240838.1 Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 3, mRNA.

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CloneID	OHu15964
Clone ID Related Accession (Same CDS sequence)	NM_001253909.2 , NM_001253909.1
Accession Version	NM_001253909.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-29
Organism	Homo sapiens(human)
Product	aldo-keto reductase family 1 member C3 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV761799.2, AK296701.1 and AL391427.10. Transcript Variant: This variant (3) lacks several exons at its 3' end, which extends into an intron compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296701.1, DC345588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGATTCCA AACACCAGTG TGTAAAGCTA AATGATGGCC ACTTCATGCC TGTATTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGG TATGCAGTTT GTATGAGCAT AAAATTGCGC TTCTGCTGTC ATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240838.1
CDS	70..486
Translation	MDSKHQCVKLNDGHFMPVLGFGTYAPPEVPRSKALEVTKLAIEAGFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSTFHRPELVRPALENSLKKAQLDYVDLYLIHSPMSLKVCSLYEHKIALLLSL

Target ORF information:

RefSeq Version	NM_001253909.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aldo-keto reductase family 1 member C3 (AKR1C3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253909.1

ORF Insert Sequence:

ATGGATTCCA AACACCAGTG TGTAAAGCTA AATGATGGCC ACTTCATGCC TGTATTGGGA 
TTTGGCACCT ATGCACCTCC AGAGGTTCCG AGAAGTAAAG CTTTGGAGGT CACAAAATTA 
GCAATAGAAG CTGGGTTCCG CCATATAGAT TCTGCTCATT TATACAATAA TGAGGAGCAG 
GTTGGACTGG CCATCCGAAG CAAGATTGCA GATGGCAGTG TGAAGAGAGA AGACATATTC 
TACACTTCAA AGCTTTGGTC CACTTTTCAT CGACCAGAGT TGGTCCGACC AGCCTTGGAA 
AACTCACTGA AGAAAGCTCA ATTGGACTAT GTTGACCTCT ATCTTATTCA TTCTCCAATG 
TCTCTAAAGG TATGCAGTTT GTATGAGCAT AAAATTGCGC TTCTGCTGTC ATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

17beta-hydroxysteroid dehydrogenase type 5 is negatively correlated to apoptosis inhibitor GRP78 and tumor-secreted protein PGK1, and modulates breast cancer cell viability and proliferation.
The Journal of steroid biochemistry and molecular biology171270-280(2017 07)
Xu D,Aka JA,Wang R,Lin SX

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AKR1C3 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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