USO1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USO1
Entrez Gene ID	8615
Full Name	USO1 vesicle transport factor
Synonyms	P115,TAP,VDP
General protein information	Preferred Names USO1 vesicle transport factor Names general vesicular transport factor p115 USO1 vesicle docking protein homolog transcytosis associated protein vesicle docking protein p115
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q21.1
Summary	The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014].
Disorder MIM:	603344

mRNA and Protein(s)

mRNA	Protein	Name
XM_006714396.3	XP_006714459.1	general vesicular transport factor p115 isoform X1
NM_003715.3	NP_003706.2	general vesicular transport factor p115 isoform 2
NM_001290049.1	NP_001276978.1	general vesicular transport factor p115 isoform 1
XM_006714396.4	XP_006714459.1	general vesicular transport factor p115 isoform X1
XM_006714397.3	XP_006714460.1	general vesicular transport factor p115 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Uso1	NP_062363.1
Danio rerio (zebrafish)	uso1	NP_956449.1
Drosophila melanogaster (fruit fly)	p115	NP_572417.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009840	XP_318953.4
Neurospora crassa	NCU01644	XP_956266.1
Canis lupus familiaris (dog)	USO1	XP_005639121.1
Bos taurus (cattle)	USO1	NP_777270.2
Gallus gallus (chicken)	USO1	NP_001026310.1
Caenorhabditis elegans (roundworm)	uso-1	NP_001040974.1
Kluyveromyces lactis	KLLA0E05281g	XP_454183.1
Homo sapiens (human)	USO1	NP_003706.1
Saccharomyces cerevisiae (baker's yeast)	USO1	NP_010225.1
	USO1	XP_001100314.2
Rattus norvegicus (Norway rat)	Uso1	NP_062252.1
Danio rerio (zebrafish)	LOC100334773	XP_002660510.1
Magnaporthe oryzae (rice blast fungus)	MGG_17317	XP_003717804.1
Pan troglodytes (chimpanzee)	USO1	XP_001153352.1
Eremothecium gossypii	AGOS_AER045C	NP_984905.1
Xenopus tropicalis (tropical clawed frog)	uso1	XP_004911135.1

Gene Ontology
Bibliography

Related articles in PubMed

USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells.
Jin Y, Dai Z
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie78264-71(2016 Mar)

Lentivirus-mediated silencing of USO1 inhibits cell proliferation and migration of human colon cancer cells.
Sui J, Li X, Xing J, Cao F, Wang H, Gong H, Zhang W
Medical oncology (Northwood, London, England)32(8)218(2015 Aug)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

P115 promotes growth of gastric cancer through interaction with macrophage migration inhibitory factor.
Li XJ, Luo Y, Yi YF
World journal of gastroenterology19(46)8619-29(2013 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

USO1 gene may be a promising target for the therapy of human multiple myeloma and its diagnosis marker.
Title: USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells.

USO1 gene has a critical role in human colon cancer: USO1 is involved in cell proliferation and cell migration of colon cancer cells.
Title: Lentivirus-mediated silencing of USO1 inhibits cell proliferation and migration of human colon cancer cells.

P115 promotes proliferation of gastric cancer cells through an interaction with MIF.
Title: P115 promotes growth of gastric cancer through interaction with macrophage migration inhibitory factor.

p115 plays a critical role in mitosis progression, implicating it as the only known golgin to regulate both mitosis and apoptosis
Title: The Golgi protein p115 associates with gamma-tubulin and plays a role in Golgi structure and mitosis progression.

a conserved tethering function of the Golgi protein p115 CTF which promotes p53-ERK interaction for the amplification of the apoptotic signal.
Title: Tethering function of the caspase cleavage fragment of Golgi protein p115 promotes apoptosis via a p53-dependent pathway.

The following USO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12526	NM_001290049.1 Latest version!	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu12747	NM_003715.3 Latest version!	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu39194	XM_006714396.4 Latest version!	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu39195	XM_006714397.3 Latest version!	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu39194	XM_006714396.3	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USO1 ( NM_001290049.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276978.1 Homo sapiens USO1 vesicle transport factor (USO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12526
Clone ID Related Accession (Same CDS sequence)	NM_001290049.1
Accession Version	NM_001290049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	general vesicular transport factor p115 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA107349.1, HY008203.1, BC032654.1, AL832010.2, AK314289.1, AL833753.1 and AW043712.1. On Feb 21, 2014 this sequence version replaced XM_006714395.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832010.2, SRR1660803.50862.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGATGAT 
GTTGAAGAAG AAAATTCCAC AAGACAGAGT GAAGATTTGG GAAGCCAATT TACAGAAATT 
TTCATTAAGC AGCAGGAAAA TGTCACTCTT CTGTTATCTT TATTGGAGGA GTTTGATTTC 
CATGTCCGCT GGCCTGGTGT GAAGCTTCTT ACTTCTCTTT TAAAACAACT AGGGCCTCAG 
GTGCAACAAA TTATTTTAGT CAGTCCTATG GGTGTTTCAA GATTGATGGA CTTACTAGCG 
GATTCCAGGG AAGTTATACG TAATGATGGC GTCTTACTAC TGCAGGCACT AACAAGAAGC 
AATGGTGCAA TCCAGAAAAT TGTTGCTTTT GAAAATGCTT TCGAGAGACT ACTGGACATT 
ATTTCAGAGG AGGGGAACAG TGATGGAGGT ATAGTAGTTG AAGATTGTTT GATTTTGCTC 
CAAAACTTAT TAAAAAACAA CAACTCCAAT CAAAATTTTT TTAAAGAAGG CTCATATATT 
CAACGTATGA AACCTTGGTT TGAAGTTGGA GATGAAAATT CTGGCTGGTC TGCACAGAAA 
GTGACCAATC TACATCTAAT GCTACAGCTT GTTCGTGTAT TGGTATCTCC CACCAACCCT 
CCTGGTGCTA CCAGTAGCTG CCAGAAGGCT ATGTTCCAGT GTGGGTTATT GCAGCAGCTT 
TGTACTATCC TAATGGCTAC TGGGGTTCCT GCTGATATCC TGACTGAGAC CATAAATACT 
GTATCAGAAG TTATTCGAGG CTGCCAAGTA AACCAAGACT ACTTTGCATC TGTAAATGCA 
CCTTCAAACC CACCAAGACC GGCAATTGTA GTACTTCTCA TGTCCATGGT TAATGAAAGG 
CAGCCATTTG TTTTGCGCTG TGCTGTTCTC TATTGTTTCC AGTGTTTCTT GTATAAAAAC 
CAAAAAGGAC AAGGAGAAAT CGTGTCAACA CTTTTACCTT CTACCATTGA TGCAACAGGT 
AATTCAGTTT CAGCTGGCCA GTTATTATGT GGAGGTTTGT TTTCTACTGA TTCACTTTCA 
AACTGGTGTG CTGCTGTGGC CCTTGCCCAT GCGTTGCAAG AAAATGCCAC CCAGAAAGAA 
CAGTTGCTCA GGGTTCAACT TGCTACAAGT ATTGGCAACC CTCCAGTTTC TTTACTTCAA 
CAGTGCACCA ATATTCTTTC ACAGGGTGAT AAGATCGACA GACGGGGAAG CAAAATACAA 
ACAAGAGTTG GATTATTAAT GTTGCTTTGT ACCTGGCTAA GCAATTGTCC CATTGCAGTA 
ACGCATTTTC TTCACAATTC AGCCAATGTT CCATTTCTTA CAGGACAAAT TGCAGAAAAT 
CTTGGAGAAG AAGAGCAGTT GGTCCAAGGC TTATGTGCCC TTTTGTTGGG CATTTCGATT 
TATTTCAATG ATAACTCACT TGAGAGCTAC ATGAAAGAGA AGCTAAAACA ACTGATTGAG 
AAGAGGATTG GCAAAGAGAA TTTCATAGAG AAACTAGGAT TTATTAGCAA ACATGAGTTG 
TATTCCAGAG CATCTCAGAA ACCCCAGCCA AACTTTCCCA GTCCAGAATA CATGATATTT 
GATCATGAGT TTACGAAGCT GGTAAAAGAA CTTGAAGGTG TTATAACTAA GGCTATTTAT 
AAGTCCAGTG AAGAAGATAA AAAAGAAGAA GAGGTGAAAA AAACATTAGA ACAGCATGAC 
AATATTGTGA CTCACTACAA AAATATGATT CGAGAGCAGG ATCTCCAACT TGAGGAATTA 
AGGCAGCAGG TTTCTACATT AAAATGTCAA AATGAACAGC TCCAGACGGC AGTCACACAG 
CAAGTATCAC AGATCCAGCA GCACAAAGAC CAGTATAATC TTCTTAAAAT ACAGCTAGGA 
AAAGACAATC AGCATCAAGG TTCTTACAGT GAGGGGGCTC AGATGAATGG CATTCAGCCA 
GAAGAAATTG GTAGATTGCG AGAAGAGATA GAAGAATTAA AACGTAATCA GGAACTTTTA 
CAAAGCCAGC TGACTGAAAA GGACTCTATG ATTGAAAATA TGAAATCTTC CCAAACATCT 
GGCACAAATG AACAGTCTTC AGCAATAGTT TCAGCTAGAG ATTCTGAACA AGTTGCAGAA 
TTAAAACAGG AACTGGCAAC TTTAAAGTCT CAGTTAAACT CACAATCTGT GGAGATCACC 
AAACTACAGA CAGAAAAGCA GGAACTGTTA CAGAAAACAG AAGCGTTTGC AAAATCAGTT 
GAGGTACAAG GAGAGACCGA GACTATAATA GCCACCAAAA CTACTGATGT AGAAGGAAGA 
CTGTCAGCAT TATTACAAGA GACCAAAGAG TTAAAGAATG AAATTAAAGC TCTGTCTGAG 
GAAAGAACTG CCATTAAAGA GCAGCTGGAT TCATCTAATA GTACCATTGC CATTTTACAA 
ACTGAGAAAG ACAAACTAGA GTTGGAAATT ACAGATTCTA AAAAAGAACA AGATGATCTC 
TTGGTGCTCT TGGCCGATCA AGATCAGAAA ATACTGTCAT TGAAGAATAA ACTCAAGGAT 
CTTGGTCATC CAGTTGAAGA AGAGGATGAA CTTGAATCTG GAGACCAAGA GGATGAGGAT 
GATGAAAGTG AAGATCCTGG CAAGGATCTA GATCATATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276978.1
CDS	299..3220
Translation	MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVDDVEEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGDKIDRRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI

Target ORF information:

RefSeq Version	NM_001290049.1
Organism	Homo sapiens(human)
Definition	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290049.1

ORF Insert Sequence:

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGATGAT 
GTTGAAGAAG AAAATTCCAC AAGACAGAGT GAAGATTTGG GAAGCCAATT TACAGAAATT 
TTCATTAAGC AGCAGGAAAA TGTCACTCTT CTGTTATCTT TATTGGAGGA GTTTGATTTC 
CATGTCCGCT GGCCTGGTGT GAAGCTTCTT ACTTCTCTTT TAAAACAACT AGGGCCTCAG 
GTGCAACAAA TTATTTTAGT CAGTCCTATG GGTGTTTCAA GATTGATGGA CTTACTAGCG 
GATTCCAGGG AAGTTATACG TAATGATGGC GTCTTACTAC TGCAGGCACT AACAAGAAGC 
AATGGTGCAA TCCAGAAAAT TGTTGCTTTT GAAAATGCTT TCGAGAGACT ACTGGACATT 
ATTTCAGAGG AGGGGAACAG TGATGGAGGT ATAGTAGTTG AAGATTGTTT GATTTTGCTC 
CAAAACTTAT TAAAAAACAA CAACTCCAAT CAAAATTTTT TTAAAGAAGG CTCATATATT 
CAACGTATGA AACCTTGGTT TGAAGTTGGA GATGAAAATT CTGGCTGGTC TGCACAGAAA 
GTGACCAATC TACATCTAAT GCTACAGCTT GTTCGTGTAT TGGTATCTCC CACCAACCCT 
CCTGGTGCTA CCAGTAGCTG CCAGAAGGCT ATGTTCCAGT GTGGGTTATT GCAGCAGCTT 
TGTACTATCC TAATGGCTAC TGGGGTTCCT GCTGATATCC TGACTGAGAC CATAAATACT 
GTATCAGAAG TTATTCGAGG CTGCCAAGTA AACCAAGACT ACTTTGCATC TGTAAATGCA 
CCTTCAAACC CACCAAGACC GGCAATTGTA GTACTTCTCA TGTCCATGGT TAATGAAAGG 
CAGCCATTTG TTTTGCGCTG TGCTGTTCTC TATTGTTTCC AGTGTTTCTT GTATAAAAAC 
CAAAAAGGAC AAGGAGAAAT CGTGTCAACA CTTTTACCTT CTACCATTGA TGCAACAGGT 
AATTCAGTTT CAGCTGGCCA GTTATTATGT GGAGGTTTGT TTTCTACTGA TTCACTTTCA 
AACTGGTGTG CTGCTGTGGC CCTTGCCCAT GCGTTGCAAG AAAATGCCAC CCAGAAAGAA 
CAGTTGCTCA GGGTTCAACT TGCTACAAGT ATTGGCAACC CTCCAGTTTC TTTACTTCAA 
CAGTGCACCA ATATTCTTTC ACAGGGTGAT AAGATCGACA GACGGGGAAG CAAAATACAA 
ACAAGAGTTG GATTATTAAT GTTGCTTTGT ACCTGGCTAA GCAATTGTCC CATTGCAGTA 
ACGCATTTTC TTCACAATTC AGCCAATGTT CCATTTCTTA CAGGACAAAT TGCAGAAAAT 
CTTGGAGAAG AAGAGCAGTT GGTCCAAGGC TTATGTGCCC TTTTGTTGGG CATTTCGATT 
TATTTCAATG ATAACTCACT TGAGAGCTAC ATGAAAGAGA AGCTAAAACA ACTGATTGAG 
AAGAGGATTG GCAAAGAGAA TTTCATAGAG AAACTAGGAT TTATTAGCAA ACATGAGTTG 
TATTCCAGAG CATCTCAGAA ACCCCAGCCA AACTTTCCCA GTCCAGAATA CATGATATTT 
GATCATGAGT TTACGAAGCT GGTAAAAGAA CTTGAAGGTG TTATAACTAA GGCTATTTAT 
AAGTCCAGTG AAGAAGATAA AAAAGAAGAA GAGGTGAAAA AAACATTAGA ACAGCATGAC 
AATATTGTGA CTCACTACAA AAATATGATT CGAGAGCAGG ATCTCCAACT TGAGGAATTA 
AGGCAGCAGG TTTCTACATT AAAATGTCAA AATGAACAGC TCCAGACGGC AGTCACACAG 
CAAGTATCAC AGATCCAGCA GCACAAAGAC CAGTATAATC TTCTTAAAAT ACAGCTAGGA 
AAAGACAATC AGCATCAAGG TTCTTACAGT GAGGGGGCTC AGATGAATGG CATTCAGCCA 
GAAGAAATTG GTAGATTGCG AGAAGAGATA GAAGAATTAA AACGTAATCA GGAACTTTTA 
CAAAGCCAGC TGACTGAAAA GGACTCTATG ATTGAAAATA TGAAATCTTC CCAAACATCT 
GGCACAAATG AACAGTCTTC AGCAATAGTT TCAGCTAGAG ATTCTGAACA AGTTGCAGAA 
TTAAAACAGG AACTGGCAAC TTTAAAGTCT CAGTTAAACT CACAATCTGT GGAGATCACC 
AAACTACAGA CAGAAAAGCA GGAACTGTTA CAGAAAACAG AAGCGTTTGC AAAATCAGTT 
GAGGTACAAG GAGAGACCGA GACTATAATA GCCACCAAAA CTACTGATGT AGAAGGAAGA 
CTGTCAGCAT TATTACAAGA GACCAAAGAG TTAAAGAATG AAATTAAAGC TCTGTCTGAG 
GAAAGAACTG CCATTAAAGA GCAGCTGGAT TCATCTAATA GTACCATTGC CATTTTACAA 
ACTGAGAAAG ACAAACTAGA GTTGGAAATT ACAGATTCTA AAAAAGAACA AGATGATCTC 
TTGGTGCTCT TGGCCGATCA AGATCAGAAA ATACTGTCAT TGAAGAATAA ACTCAAGGAT 
CTTGGTCATC CAGTTGAAGA AGAGGATGAA CTTGAATCTG GAGACCAAGA GGATGAGGAT 
GATGAAAGTG AAGATCCTGG CAAGGATCTA GATCATATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USO1 ( NM_003715.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003706.2 Homo sapiens USO1 vesicle transport factor (USO1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12747
Clone ID Related Accession (Same CDS sequence)	NM_003715.3
Accession Version	NM_003715.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	general vesicular transport factor p115 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA107349.1, HY008203.1, BC032654.1, AK314289.1, AL833753.1 and AW043712.1. On Feb 21, 2014 this sequence version replaced NM_003715.2. Transcript Variant: This variant (2) lacks two exons in the central coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.172586.1, SRR1803611.227525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGAAGAA 
AATTCCACAA GACAGAGTGA AGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CAGGAAAATG TCACTCTTCT GTTATCTTTA TTGGAGGAGT TTGATTTCCA TGTCCGCTGG 
CCTGGTGTGA AGCTTCTTAC TTCTCTTTTA AAACAACTAG GGCCTCAGGT GCAACAAATT 
ATTTTAGTCA GTCCTATGGG TGTTTCAAGA TTGATGGACT TACTAGCGGA TTCCAGGGAA 
GTTATACGTA ATGATGGCGT CTTACTACTG CAGGCACTAA CAAGAAGCAA TGGTGCAATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TTCAGAGGAG 
GGGAACAGTG ATGGAGGTAT AGTAGTTGAA GATTGTTTGA TTTTGCTCCA AAACTTATTA 
AAAAACAACA ACTCCAATCA AAATTTTTTT AAAGAAGGCT CATATATTCA ACGTATGAAA 
CCTTGGTTTG AAGTTGGAGA TGAAAATTCT GGCTGGTCTG CACAGAAAGT GACCAATCTA 
CATCTAATGC TACAGCTTGT TCGTGTATTG GTATCTCCCA CCAACCCTCC TGGTGCTACC 
AGTAGCTGCC AGAAGGCTAT GTTCCAGTGT GGGTTATTGC AGCAGCTTTG TACTATCCTA 
ATGGCTACTG GGGTTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA CCAAGACTAC TTTGCATCTG TAAATGCACC TTCAAACCCA 
CCAAGACCGG CAATTGTAGT ACTTCTCATG TCCATGGTTA ATGAAAGGCA GCCATTTGTT 
TTGCGCTGTG CTGTTCTCTA TTGTTTCCAG TGTTTCTTGT ATAAAAACCA AAAAGGACAA 
GGAGAAATCG TGTCAACACT TTTACCTTCT ACCATTGATG CAACAGGTAA TTCAGTTTCA 
GCTGGCCAGT TATTATGTGG AGGTTTGTTT TCTACTGATT CACTTTCAAA CTGGTGTGCT 
GCTGTGGCCC TTGCCCATGC GTTGCAAGAA AATGCCACCC AGAAAGAACA GTTGCTCAGG 
GTTCAACTTG CTACAAGTAT TGGCAACCCT CCAGTTTCTT TACTTCAACA GTGCACCAAT 
ATTCTTTCAC AGGGAAGCAA AATACAAACA AGAGTTGGAT TATTAATGTT GCTTTGTACC 
TGGCTAAGCA ATTGTCCCAT TGCAGTAACG CATTTTCTTC ACAATTCAGC CAATGTTCCA 
TTTCTTACAG GACAAATTGC AGAAAATCTT GGAGAAGAAG AGCAGTTGGT CCAAGGCTTA 
TGTGCCCTTT TGTTGGGCAT TTCGATTTAT TTCAATGATA ACTCACTTGA GAGCTACATG 
AAAGAGAAGC TAAAACAACT GATTGAGAAG AGGATTGGCA AAGAGAATTT CATAGAGAAA 
CTAGGATTTA TTAGCAAACA TGAGTTGTAT TCCAGAGCAT CTCAGAAACC CCAGCCAAAC 
TTTCCCAGTC CAGAATACAT GATATTTGAT CATGAGTTTA CGAAGCTGGT AAAAGAACTT 
GAAGGTGTTA TAACTAAGGC TATTTATAAG TCCAGTGAAG AAGATAAAAA AGAAGAAGAG 
GTGAAAAAAA CATTAGAACA GCATGACAAT ATTGTGACTC ACTACAAAAA TATGATTCGA 
GAGCAGGATC TCCAACTTGA GGAATTAAGG CAGCAGGTTT CTACATTAAA ATGTCAAAAT 
GAACAGCTCC AGACGGCAGT CACACAGCAA GTATCACAGA TCCAGCAGCA CAAAGACCAG 
TATAATCTTC TTAAAATACA GCTAGGAAAA GACAATCAGC ATCAAGGTTC TTACAGTGAG 
GGGGCTCAGA TGAATGGCAT TCAGCCAGAA GAAATTGGTA GATTGCGAGA AGAGATAGAA 
GAATTAAAAC GTAATCAGGA ACTTTTACAA AGCCAGCTGA CTGAAAAGGA CTCTATGATT 
GAAAATATGA AATCTTCCCA AACATCTGGC ACAAATGAAC AGTCTTCAGC AATAGTTTCA 
GCTAGAGATT CTGAACAAGT TGCAGAATTA AAACAGGAAC TGGCAACTTT AAAGTCTCAG 
TTAAACTCAC AATCTGTGGA GATCACCAAA CTACAGACAG AAAAGCAGGA ACTGTTACAG 
AAAACAGAAG CGTTTGCAAA ATCAGTTGAG GTACAAGGAG AGACCGAGAC TATAATAGCC 
ACCAAAACTA CTGATGTAGA AGGAAGACTG TCAGCATTAT TACAAGAGAC CAAAGAGTTA 
AAGAATGAAA TTAAAGCTCT GTCTGAGGAA AGAACTGCCA TTAAAGAGCA GCTGGATTCA 
TCTAATAGTA CCATTGCCAT TTTACAAACT GAGAAAGACA AACTAGAGTT GGAAATTACA 
GATTCTAAAA AAGAACAAGA TGATCTCTTG GTGCTCTTGG CCGATCAAGA TCAGAAAATA 
CTGTCATTGA AGAATAAACT CAAGGATCTT GGTCATCCAG TTGAAGAAGA GGATGAACTT 
GAATCTGGAG ACCAAGAGGA TGAGGATGAT GAAAGTGAAG ATCCTGGCAA GGATCTAGAT 
CATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003706.2
CDS	299..3187
Translation	MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI

Target ORF information:

RefSeq Version	NM_003715.3
Organism	Homo sapiens(human)
Definition	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003715.3

ORF Insert Sequence:

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGAAGAA 
AATTCCACAA GACAGAGTGA AGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CAGGAAAATG TCACTCTTCT GTTATCTTTA TTGGAGGAGT TTGATTTCCA TGTCCGCTGG 
CCTGGTGTGA AGCTTCTTAC TTCTCTTTTA AAACAACTAG GGCCTCAGGT GCAACAAATT 
ATTTTAGTCA GTCCTATGGG TGTTTCAAGA TTGATGGACT TACTAGCGGA TTCCAGGGAA 
GTTATACGTA ATGATGGCGT CTTACTACTG CAGGCACTAA CAAGAAGCAA TGGTGCAATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TTCAGAGGAG 
GGGAACAGTG ATGGAGGTAT AGTAGTTGAA GATTGTTTGA TTTTGCTCCA AAACTTATTA 
AAAAACAACA ACTCCAATCA AAATTTTTTT AAAGAAGGCT CATATATTCA ACGTATGAAA 
CCTTGGTTTG AAGTTGGAGA TGAAAATTCT GGCTGGTCTG CACAGAAAGT GACCAATCTA 
CATCTAATGC TACAGCTTGT TCGTGTATTG GTATCTCCCA CCAACCCTCC TGGTGCTACC 
AGTAGCTGCC AGAAGGCTAT GTTCCAGTGT GGGTTATTGC AGCAGCTTTG TACTATCCTA 
ATGGCTACTG GGGTTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA CCAAGACTAC TTTGCATCTG TAAATGCACC TTCAAACCCA 
CCAAGACCGG CAATTGTAGT ACTTCTCATG TCCATGGTTA ATGAAAGGCA GCCATTTGTT 
TTGCGCTGTG CTGTTCTCTA TTGTTTCCAG TGTTTCTTGT ATAAAAACCA AAAAGGACAA 
GGAGAAATCG TGTCAACACT TTTACCTTCT ACCATTGATG CAACAGGTAA TTCAGTTTCA 
GCTGGCCAGT TATTATGTGG AGGTTTGTTT TCTACTGATT CACTTTCAAA CTGGTGTGCT 
GCTGTGGCCC TTGCCCATGC GTTGCAAGAA AATGCCACCC AGAAAGAACA GTTGCTCAGG 
GTTCAACTTG CTACAAGTAT TGGCAACCCT CCAGTTTCTT TACTTCAACA GTGCACCAAT 
ATTCTTTCAC AGGGAAGCAA AATACAAACA AGAGTTGGAT TATTAATGTT GCTTTGTACC 
TGGCTAAGCA ATTGTCCCAT TGCAGTAACG CATTTTCTTC ACAATTCAGC CAATGTTCCA 
TTTCTTACAG GACAAATTGC AGAAAATCTT GGAGAAGAAG AGCAGTTGGT CCAAGGCTTA 
TGTGCCCTTT TGTTGGGCAT TTCGATTTAT TTCAATGATA ACTCACTTGA GAGCTACATG 
AAAGAGAAGC TAAAACAACT GATTGAGAAG AGGATTGGCA AAGAGAATTT CATAGAGAAA 
CTAGGATTTA TTAGCAAACA TGAGTTGTAT TCCAGAGCAT CTCAGAAACC CCAGCCAAAC 
TTTCCCAGTC CAGAATACAT GATATTTGAT CATGAGTTTA CGAAGCTGGT AAAAGAACTT 
GAAGGTGTTA TAACTAAGGC TATTTATAAG TCCAGTGAAG AAGATAAAAA AGAAGAAGAG 
GTGAAAAAAA CATTAGAACA GCATGACAAT ATTGTGACTC ACTACAAAAA TATGATTCGA 
GAGCAGGATC TCCAACTTGA GGAATTAAGG CAGCAGGTTT CTACATTAAA ATGTCAAAAT 
GAACAGCTCC AGACGGCAGT CACACAGCAA GTATCACAGA TCCAGCAGCA CAAAGACCAG 
TATAATCTTC TTAAAATACA GCTAGGAAAA GACAATCAGC ATCAAGGTTC TTACAGTGAG 
GGGGCTCAGA TGAATGGCAT TCAGCCAGAA GAAATTGGTA GATTGCGAGA AGAGATAGAA 
GAATTAAAAC GTAATCAGGA ACTTTTACAA AGCCAGCTGA CTGAAAAGGA CTCTATGATT 
GAAAATATGA AATCTTCCCA AACATCTGGC ACAAATGAAC AGTCTTCAGC AATAGTTTCA 
GCTAGAGATT CTGAACAAGT TGCAGAATTA AAACAGGAAC TGGCAACTTT AAAGTCTCAG 
TTAAACTCAC AATCTGTGGA GATCACCAAA CTACAGACAG AAAAGCAGGA ACTGTTACAG 
AAAACAGAAG CGTTTGCAAA ATCAGTTGAG GTACAAGGAG AGACCGAGAC TATAATAGCC 
ACCAAAACTA CTGATGTAGA AGGAAGACTG TCAGCATTAT TACAAGAGAC CAAAGAGTTA 
AAGAATGAAA TTAAAGCTCT GTCTGAGGAA AGAACTGCCA TTAAAGAGCA GCTGGATTCA 
TCTAATAGTA CCATTGCCAT TTTACAAACT GAGAAAGACA AACTAGAGTT GGAAATTACA 
GATTCTAAAA AAGAACAAGA TGATCTCTTG GTGCTCTTGG CCGATCAAGA TCAGAAAATA 
CTGTCATTGA AGAATAAACT CAAGGATCTT GGTCATCCAG TTGAAGAAGA GGATGAACTT 
GAATCTGGAG ACCAAGAGGA TGAGGATGAT GAAAGTGAAG ATCCTGGCAA GGATCTAGAT 
CATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USO1 ( XM_006714396.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714459.1 Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu39194
Clone ID Related Accession (Same CDS sequence)	XM_006714396.3 , XM_006714396.4
Accession Version	XM_006714396.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	general vesicular transport factor p115 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGAAGAA 
AATTCCACAA GACAGAGTGA AGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CAGGAAAATG TCACTCTTCT GTTATCTTTA TTGGAGGAGT TTGATTTCCA TGTCCGCTGG 
CCTGGTGTGA AGCTTCTTAC TTCTCTTTTA AAACAACTAG GGCCTCAGGT GCAACAAATT 
ATTTTAGTCA GTCCTATGGG TGTTTCAAGA TTGATGGACT TACTAGCGGA TTCCAGGGAA 
GTTATACGTA ATGATGGCGT CTTACTACTG CAGGCACTAA CAAGAAGCAA TGGTGCAATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TTCAGAGGAG 
GGGAACAGTG ATGGAGGTAT AGTAGTTGAA GATTGTTTGA TTTTGCTCCA AAACTTATTA 
AAAAACAACA ACTCCAATCA AAATTTTTTT AAAGAAGGCT CATATATTCA ACGTATGAAA 
CCTTGGTTTG AAGTTGGAGA TGAAAATTCT GGCTGGTCTG CACAGAAAGT GACCAATCTA 
CATCTAATGC TACAGCTTGT TCGTGTATTG GTATCTCCCA CCAACCCTCC TGGTGCTACC 
AGTAGCTGCC AGAAGGCTAT GTTCCAGTGT GGGTTATTGC AGCAGCTTTG TACTATCCTA 
ATGGCTACTG GGGTTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA CCAAGACTAC TTTGCATCTG TAAATGCACC TTCAAACCCA 
CCAAGACCGG CAATTGTAGT ACTTCTCATG TCCATGGTTA ATGAAAGGCA GCCATTTGTT 
TTGCGCTGTG CTGTTCTCTA TTGTTTCCAG TGTTTCTTGT ATAAAAACCA AAAAGGACAA 
GGAGAAATCG TGTCAACACT TTTACCTTCT ACCATTGATG CAACAGGTAA TTCAGTTTCA 
GCTGGCCAGT TATTATGTGG AGGTTTGTTT TCTACTGATT CACTTTCAAA CTGGTGTGCT 
GCTGTGGCCC TTGCCCATGC GTTGCAAGAA AATGCCACCC AGAAAGAACA GTTGCTCAGG 
GTTCAACTTG CTACAAGTAT TGGCAACCCT CCAGTTTCTT TACTTCAACA GTGCACCAAT 
ATTCTTTCAC AGGGTGATAA GATCGACAGA CGGGGAAGCA AAATACAAAC AAGAGTTGGA 
TTATTAATGT TGCTTTGTAC CTGGCTAAGC AATTGTCCCA TTGCAGTAAC GCATTTTCTT 
CACAATTCAG CCAATGTTCC ATTTCTTACA GGACAAATTG CAGAAAATCT TGGAGAAGAA 
GAGCAGTTGG TCCAAGGCTT ATGTGCCCTT TTGTTGGGCA TTTCGATTTA TTTCAATGAT 
AACTCACTTG AGAGCTACAT GAAAGAGAAG CTAAAACAAC TGATTGAGAA GAGGATTGGC 
AAAGAGAATT TCATAGAGAA ACTAGGATTT ATTAGCAAAC ATGAGTTGTA TTCCAGAGCA 
TCTCAGAAAC CCCAGCCAAA CTTTCCCAGT CCAGAATACA TGATATTTGA TCATGAGTTT 
ACGAAGCTGG TAAAAGAACT TGAAGGTGTT ATAACTAAGG CTATTTATAA GTCCAGTGAA 
GAAGATAAAA AAGAAGAAGA GGTGAAAAAA ACATTAGAAC AGCATGACAA TATTGTGACT 
CACTACAAAA ATATGATTCG AGAGCAGGAT CTCCAACTTG AGGAATTAAG GCAGCAGGTT 
TCTACATTAA AATGTCAAAA TGAACAGCTC CAGACGGCAG TCACACAGCA AGTATCACAG 
ATCCAGCAGC ACAAAGACCA GTATAATCTT CTTAAAATAC AGCTAGGAAA AGACAATCAG 
CATCAAGGTT CTTACAGTGA GGGGGCTCAG ATGAATGGCA TTCAGCCAGA AGAAATTGGT 
AGATTGCGAG AAGAGATAGA AGAATTAAAA CGTAATCAGG AACTTTTACA AAGCCAGCTG 
ACTGAAAAGG ACTCTATGAT TGAAAATATG AAATCTTCCC AAACATCTGG CACAAATGAA 
CAGTCTTCAG CAATAGTTTC AGCTAGAGAT TCTGAACAAG TTGCAGAATT AAAACAGGAA 
CTGGCAACTT TAAAGTCTCA GTTAAACTCA CAATCTGTGG AGATCACCAA ACTACAGACA 
GAAAAGCAGG AACTGTTACA GAAAACAGAA GCGTTTGCAA AATCAGTTGA GGTACAAGGA 
GAGACCGAGA CTATAATAGC CACCAAAACT ACTGATGTAG AAGGAAGACT GTCAGCATTA 
TTACAAGAGA CCAAAGAGTT AAAGAATGAA ATTAAAGCTC TGTCTGAGGA AAGAACTGCC 
ATTAAAGAGC AGCTGGATTC ATCTAATAGT ACCATTGCCA TTTTACAAAC TGAGAAAGAC 
AAACTAGAGT TGGAAATTAC AGATTCTAAA AAAGAACAAG ATGATCTCTT GGTGCTCTTG 
GCCGATCAAG ATCAGAAAAT ACTGTCATTG AAGAATAAAC TCAAGGATCT TGGTCATCCA 
GTTGAAGAAG AGGATGAACT TGAATCTGGA GACCAAGAGG ATGAGGATGA TGAAAGTGAA 
GATCCTGGCA AGGATCTAGA TCATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714459.1
CDS	294..3203
Translation	MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGDKIDRRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI

Target ORF information:

RefSeq Version	XM_006714396.3
Organism	Homo sapiens(human)
Definition	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714396.3

ORF Insert Sequence:

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGAAGAA 
AATTCCACAA GACAGAGTGA AGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CAGGAAAATG TCACTCTTCT GTTATCTTTA TTGGAGGAGT TTGATTTCCA TGTCCGCTGG 
CCTGGTGTGA AGCTTCTTAC TTCTCTTTTA AAACAACTAG GGCCTCAGGT GCAACAAATT 
ATTTTAGTCA GTCCTATGGG TGTTTCAAGA TTGATGGACT TACTAGCGGA TTCCAGGGAA 
GTTATACGTA ATGATGGCGT CTTACTACTG CAGGCACTAA CAAGAAGCAA TGGTGCAATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TTCAGAGGAG 
GGGAACAGTG ATGGAGGTAT AGTAGTTGAA GATTGTTTGA TTTTGCTCCA AAACTTATTA 
AAAAACAACA ACTCCAATCA AAATTTTTTT AAAGAAGGCT CATATATTCA ACGTATGAAA 
CCTTGGTTTG AAGTTGGAGA TGAAAATTCT GGCTGGTCTG CACAGAAAGT GACCAATCTA 
CATCTAATGC TACAGCTTGT TCGTGTATTG GTATCTCCCA CCAACCCTCC TGGTGCTACC 
AGTAGCTGCC AGAAGGCTAT GTTCCAGTGT GGGTTATTGC AGCAGCTTTG TACTATCCTA 
ATGGCTACTG GGGTTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA CCAAGACTAC TTTGCATCTG TAAATGCACC TTCAAACCCA 
CCAAGACCGG CAATTGTAGT ACTTCTCATG TCCATGGTTA ATGAAAGGCA GCCATTTGTT 
TTGCGCTGTG CTGTTCTCTA TTGTTTCCAG TGTTTCTTGT ATAAAAACCA AAAAGGACAA 
GGAGAAATCG TGTCAACACT TTTACCTTCT ACCATTGATG CAACAGGTAA TTCAGTTTCA 
GCTGGCCAGT TATTATGTGG AGGTTTGTTT TCTACTGATT CACTTTCAAA CTGGTGTGCT 
GCTGTGGCCC TTGCCCATGC GTTGCAAGAA AATGCCACCC AGAAAGAACA GTTGCTCAGG 
GTTCAACTTG CTACAAGTAT TGGCAACCCT CCAGTTTCTT TACTTCAACA GTGCACCAAT 
ATTCTTTCAC AGGGTGATAA GATCGACAGA CGGGGAAGCA AAATACAAAC AAGAGTTGGA 
TTATTAATGT TGCTTTGTAC CTGGCTAAGC AATTGTCCCA TTGCAGTAAC GCATTTTCTT 
CACAATTCAG CCAATGTTCC ATTTCTTACA GGACAAATTG CAGAAAATCT TGGAGAAGAA 
GAGCAGTTGG TCCAAGGCTT ATGTGCCCTT TTGTTGGGCA TTTCGATTTA TTTCAATGAT 
AACTCACTTG AGAGCTACAT GAAAGAGAAG CTAAAACAAC TGATTGAGAA GAGGATTGGC 
AAAGAGAATT TCATAGAGAA ACTAGGATTT ATTAGCAAAC ATGAGTTGTA TTCCAGAGCA 
TCTCAGAAAC CCCAGCCAAA CTTTCCCAGT CCAGAATACA TGATATTTGA TCATGAGTTT 
ACGAAGCTGG TAAAAGAACT TGAAGGTGTT ATAACTAAGG CTATTTATAA GTCCAGTGAA 
GAAGATAAAA AAGAAGAAGA GGTGAAAAAA ACATTAGAAC AGCATGACAA TATTGTGACT 
CACTACAAAA ATATGATTCG AGAGCAGGAT CTCCAACTTG AGGAATTAAG GCAGCAGGTT 
TCTACATTAA AATGTCAAAA TGAACAGCTC CAGACGGCAG TCACACAGCA AGTATCACAG 
ATCCAGCAGC ACAAAGACCA GTATAATCTT CTTAAAATAC AGCTAGGAAA AGACAATCAG 
CATCAAGGTT CTTACAGTGA GGGGGCTCAG ATGAATGGCA TTCAGCCAGA AGAAATTGGT 
AGATTGCGAG AAGAGATAGA AGAATTAAAA CGTAATCAGG AACTTTTACA AAGCCAGCTG 
ACTGAAAAGG ACTCTATGAT TGAAAATATG AAATCTTCCC AAACATCTGG CACAAATGAA 
CAGTCTTCAG CAATAGTTTC AGCTAGAGAT TCTGAACAAG TTGCAGAATT AAAACAGGAA 
CTGGCAACTT TAAAGTCTCA GTTAAACTCA CAATCTGTGG AGATCACCAA ACTACAGACA 
GAAAAGCAGG AACTGTTACA GAAAACAGAA GCGTTTGCAA AATCAGTTGA GGTACAAGGA 
GAGACCGAGA CTATAATAGC CACCAAAACT ACTGATGTAG AAGGAAGACT GTCAGCATTA 
TTACAAGAGA CCAAAGAGTT AAAGAATGAA ATTAAAGCTC TGTCTGAGGA AAGAACTGCC 
ATTAAAGAGC AGCTGGATTC ATCTAATAGT ACCATTGCCA TTTTACAAAC TGAGAAAGAC 
AAACTAGAGT TGGAAATTAC AGATTCTAAA AAAGAACAAG ATGATCTCTT GGTGCTCTTG 
GCCGATCAAG ATCAGAAAAT ACTGTCATTG AAGAATAAAC TCAAGGATCT TGGTCATCCA 
GTTGAAGAAG AGGATGAACT TGAATCTGGA GACCAAGAGG ATGAGGATGA TGAAAGTGAA 
GATCCTGGCA AGGATCTAGA TCATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USO1 ( XM_006714396.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714459.1 Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu39194
Clone ID Related Accession (Same CDS sequence)	XM_006714396.3 , XM_006714396.4
Accession Version	XM_006714396.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	general vesicular transport factor p115 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006714396.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGAAGAA 
AATTCCACAA GACAGAGTGA AGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CAGGAAAATG TCACTCTTCT GTTATCTTTA TTGGAGGAGT TTGATTTCCA TGTCCGCTGG 
CCTGGTGTGA AGCTTCTTAC TTCTCTTTTA AAACAACTAG GGCCTCAGGT GCAACAAATT 
ATTTTAGTCA GTCCTATGGG TGTTTCAAGA TTGATGGACT TACTAGCGGA TTCCAGGGAA 
GTTATACGTA ATGATGGCGT CTTACTACTG CAGGCACTAA CAAGAAGCAA TGGTGCAATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TTCAGAGGAG 
GGGAACAGTG ATGGAGGTAT AGTAGTTGAA GATTGTTTGA TTTTGCTCCA AAACTTATTA 
AAAAACAACA ACTCCAATCA AAATTTTTTT AAAGAAGGCT CATATATTCA ACGTATGAAA 
CCTTGGTTTG AAGTTGGAGA TGAAAATTCT GGCTGGTCTG CACAGAAAGT GACCAATCTA 
CATCTAATGC TACAGCTTGT TCGTGTATTG GTATCTCCCA CCAACCCTCC TGGTGCTACC 
AGTAGCTGCC AGAAGGCTAT GTTCCAGTGT GGGTTATTGC AGCAGCTTTG TACTATCCTA 
ATGGCTACTG GGGTTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA CCAAGACTAC TTTGCATCTG TAAATGCACC TTCAAACCCA 
CCAAGACCGG CAATTGTAGT ACTTCTCATG TCCATGGTTA ATGAAAGGCA GCCATTTGTT 
TTGCGCTGTG CTGTTCTCTA TTGTTTCCAG TGTTTCTTGT ATAAAAACCA AAAAGGACAA 
GGAGAAATCG TGTCAACACT TTTACCTTCT ACCATTGATG CAACAGGTAA TTCAGTTTCA 
GCTGGCCAGT TATTATGTGG AGGTTTGTTT TCTACTGATT CACTTTCAAA CTGGTGTGCT 
GCTGTGGCCC TTGCCCATGC GTTGCAAGAA AATGCCACCC AGAAAGAACA GTTGCTCAGG 
GTTCAACTTG CTACAAGTAT TGGCAACCCT CCAGTTTCTT TACTTCAACA GTGCACCAAT 
ATTCTTTCAC AGGGTGATAA GATCGACAGA CGGGGAAGCA AAATACAAAC AAGAGTTGGA 
TTATTAATGT TGCTTTGTAC CTGGCTAAGC AATTGTCCCA TTGCAGTAAC GCATTTTCTT 
CACAATTCAG CCAATGTTCC ATTTCTTACA GGACAAATTG CAGAAAATCT TGGAGAAGAA 
GAGCAGTTGG TCCAAGGCTT ATGTGCCCTT TTGTTGGGCA TTTCGATTTA TTTCAATGAT 
AACTCACTTG AGAGCTACAT GAAAGAGAAG CTAAAACAAC TGATTGAGAA GAGGATTGGC 
AAAGAGAATT TCATAGAGAA ACTAGGATTT ATTAGCAAAC ATGAGTTGTA TTCCAGAGCA 
TCTCAGAAAC CCCAGCCAAA CTTTCCCAGT CCAGAATACA TGATATTTGA TCATGAGTTT 
ACGAAGCTGG TAAAAGAACT TGAAGGTGTT ATAACTAAGG CTATTTATAA GTCCAGTGAA 
GAAGATAAAA AAGAAGAAGA GGTGAAAAAA ACATTAGAAC AGCATGACAA TATTGTGACT 
CACTACAAAA ATATGATTCG AGAGCAGGAT CTCCAACTTG AGGAATTAAG GCAGCAGGTT 
TCTACATTAA AATGTCAAAA TGAACAGCTC CAGACGGCAG TCACACAGCA AGTATCACAG 
ATCCAGCAGC ACAAAGACCA GTATAATCTT CTTAAAATAC AGCTAGGAAA AGACAATCAG 
CATCAAGGTT CTTACAGTGA GGGGGCTCAG ATGAATGGCA TTCAGCCAGA AGAAATTGGT 
AGATTGCGAG AAGAGATAGA AGAATTAAAA CGTAATCAGG AACTTTTACA AAGCCAGCTG 
ACTGAAAAGG ACTCTATGAT TGAAAATATG AAATCTTCCC AAACATCTGG CACAAATGAA 
CAGTCTTCAG CAATAGTTTC AGCTAGAGAT TCTGAACAAG TTGCAGAATT AAAACAGGAA 
CTGGCAACTT TAAAGTCTCA GTTAAACTCA CAATCTGTGG AGATCACCAA ACTACAGACA 
GAAAAGCAGG AACTGTTACA GAAAACAGAA GCGTTTGCAA AATCAGTTGA GGTACAAGGA 
GAGACCGAGA CTATAATAGC CACCAAAACT ACTGATGTAG AAGGAAGACT GTCAGCATTA 
TTACAAGAGA CCAAAGAGTT AAAGAATGAA ATTAAAGCTC TGTCTGAGGA AAGAACTGCC 
ATTAAAGAGC AGCTGGATTC ATCTAATAGT ACCATTGCCA TTTTACAAAC TGAGAAAGAC 
AAACTAGAGT TGGAAATTAC AGATTCTAAA AAAGAACAAG ATGATCTCTT GGTGCTCTTG 
GCCGATCAAG ATCAGAAAAT ACTGTCATTG AAGAATAAAC TCAAGGATCT TGGTCATCCA 
GTTGAAGAAG AGGATGAACT TGAATCTGGA GACCAAGAGG ATGAGGATGA TGAAAGTGAA 
GATCCTGGCA AGGATCTAGA TCATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714459.1
CDS	299..3208
Translation	MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGDKIDRRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI

Target ORF information:

RefSeq Version	XM_006714396.4
Organism	Homo sapiens(human)
Definition	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714396.4

ORF Insert Sequence:

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGAAGAA 
AATTCCACAA GACAGAGTGA AGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CAGGAAAATG TCACTCTTCT GTTATCTTTA TTGGAGGAGT TTGATTTCCA TGTCCGCTGG 
CCTGGTGTGA AGCTTCTTAC TTCTCTTTTA AAACAACTAG GGCCTCAGGT GCAACAAATT 
ATTTTAGTCA GTCCTATGGG TGTTTCAAGA TTGATGGACT TACTAGCGGA TTCCAGGGAA 
GTTATACGTA ATGATGGCGT CTTACTACTG CAGGCACTAA CAAGAAGCAA TGGTGCAATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TTCAGAGGAG 
GGGAACAGTG ATGGAGGTAT AGTAGTTGAA GATTGTTTGA TTTTGCTCCA AAACTTATTA 
AAAAACAACA ACTCCAATCA AAATTTTTTT AAAGAAGGCT CATATATTCA ACGTATGAAA 
CCTTGGTTTG AAGTTGGAGA TGAAAATTCT GGCTGGTCTG CACAGAAAGT GACCAATCTA 
CATCTAATGC TACAGCTTGT TCGTGTATTG GTATCTCCCA CCAACCCTCC TGGTGCTACC 
AGTAGCTGCC AGAAGGCTAT GTTCCAGTGT GGGTTATTGC AGCAGCTTTG TACTATCCTA 
ATGGCTACTG GGGTTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA CCAAGACTAC TTTGCATCTG TAAATGCACC TTCAAACCCA 
CCAAGACCGG CAATTGTAGT ACTTCTCATG TCCATGGTTA ATGAAAGGCA GCCATTTGTT 
TTGCGCTGTG CTGTTCTCTA TTGTTTCCAG TGTTTCTTGT ATAAAAACCA AAAAGGACAA 
GGAGAAATCG TGTCAACACT TTTACCTTCT ACCATTGATG CAACAGGTAA TTCAGTTTCA 
GCTGGCCAGT TATTATGTGG AGGTTTGTTT TCTACTGATT CACTTTCAAA CTGGTGTGCT 
GCTGTGGCCC TTGCCCATGC GTTGCAAGAA AATGCCACCC AGAAAGAACA GTTGCTCAGG 
GTTCAACTTG CTACAAGTAT TGGCAACCCT CCAGTTTCTT TACTTCAACA GTGCACCAAT 
ATTCTTTCAC AGGGTGATAA GATCGACAGA CGGGGAAGCA AAATACAAAC AAGAGTTGGA 
TTATTAATGT TGCTTTGTAC CTGGCTAAGC AATTGTCCCA TTGCAGTAAC GCATTTTCTT 
CACAATTCAG CCAATGTTCC ATTTCTTACA GGACAAATTG CAGAAAATCT TGGAGAAGAA 
GAGCAGTTGG TCCAAGGCTT ATGTGCCCTT TTGTTGGGCA TTTCGATTTA TTTCAATGAT 
AACTCACTTG AGAGCTACAT GAAAGAGAAG CTAAAACAAC TGATTGAGAA GAGGATTGGC 
AAAGAGAATT TCATAGAGAA ACTAGGATTT ATTAGCAAAC ATGAGTTGTA TTCCAGAGCA 
TCTCAGAAAC CCCAGCCAAA CTTTCCCAGT CCAGAATACA TGATATTTGA TCATGAGTTT 
ACGAAGCTGG TAAAAGAACT TGAAGGTGTT ATAACTAAGG CTATTTATAA GTCCAGTGAA 
GAAGATAAAA AAGAAGAAGA GGTGAAAAAA ACATTAGAAC AGCATGACAA TATTGTGACT 
CACTACAAAA ATATGATTCG AGAGCAGGAT CTCCAACTTG AGGAATTAAG GCAGCAGGTT 
TCTACATTAA AATGTCAAAA TGAACAGCTC CAGACGGCAG TCACACAGCA AGTATCACAG 
ATCCAGCAGC ACAAAGACCA GTATAATCTT CTTAAAATAC AGCTAGGAAA AGACAATCAG 
CATCAAGGTT CTTACAGTGA GGGGGCTCAG ATGAATGGCA TTCAGCCAGA AGAAATTGGT 
AGATTGCGAG AAGAGATAGA AGAATTAAAA CGTAATCAGG AACTTTTACA AAGCCAGCTG 
ACTGAAAAGG ACTCTATGAT TGAAAATATG AAATCTTCCC AAACATCTGG CACAAATGAA 
CAGTCTTCAG CAATAGTTTC AGCTAGAGAT TCTGAACAAG TTGCAGAATT AAAACAGGAA 
CTGGCAACTT TAAAGTCTCA GTTAAACTCA CAATCTGTGG AGATCACCAA ACTACAGACA 
GAAAAGCAGG AACTGTTACA GAAAACAGAA GCGTTTGCAA AATCAGTTGA GGTACAAGGA 
GAGACCGAGA CTATAATAGC CACCAAAACT ACTGATGTAG AAGGAAGACT GTCAGCATTA 
TTACAAGAGA CCAAAGAGTT AAAGAATGAA ATTAAAGCTC TGTCTGAGGA AAGAACTGCC 
ATTAAAGAGC AGCTGGATTC ATCTAATAGT ACCATTGCCA TTTTACAAAC TGAGAAAGAC 
AAACTAGAGT TGGAAATTAC AGATTCTAAA AAAGAACAAG ATGATCTCTT GGTGCTCTTG 
GCCGATCAAG ATCAGAAAAT ACTGTCATTG AAGAATAAAC TCAAGGATCT TGGTCATCCA 
GTTGAAGAAG AGGATGAACT TGAATCTGGA GACCAAGAGG ATGAGGATGA TGAAAGTGAA 
GATCCTGGCA AGGATCTAGA TCATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USO1 ( XM_006714397.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714460.1 Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X2, mRNA.

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CloneID	OHu39195
Clone ID Related Accession (Same CDS sequence)	XM_006714397.3
Accession Version	XM_006714397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	general vesicular transport factor p115 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGATGAT 
GTTGAAGAAG AAAATTCCAC AAGACAGAGT GAAGATTTGG GAAGCCAATT TACAGAAATT 
TTCATTAAGC AGCAGGAAAA TGTCACTCTT CTGTTATCTT TATTGGAGGA GTTTGATTTC 
CATGTCCGCT GGCCTGGTGT GAAGCTTCTT ACTTCTCTTT TAAAACAACT AGGGCCTCAG 
GTGCAACAAA TTATTTTAGT CAGTCCTATG GGTGTTTCAA GATTGATGGA CTTACTAGCG 
GATTCCAGGG AAGTTATACG TAATGATGGC GTCTTACTAC TGCAGGCACT AACAAGAAGC 
AATGGTGCAA TCCAGAAAAT TGTTGCTTTT GAAAATGCTT TCGAGAGACT ACTGGACATT 
ATTTCAGAGG AGGGGAACAG TGATGGAGGT ATAGTAGTTG AAGATTGTTT GATTTTGCTC 
CAAAACTTAT TAAAAAACAA CAACTCCAAT CAAAATTTTT TTAAAGAAGG CTCATATATT 
CAACGTATGA AACCTTGGTT TGAAGTTGGA GATGAAAATT CTGGCTGGTC TGCACAGAAA 
GTGACCAATC TACATCTAAT GCTACAGCTT GTTCGTGTAT TGGTATCTCC CACCAACCCT 
CCTGGTGCTA CCAGTAGCTG CCAGAAGGCT ATGTTCCAGT GTGGGTTATT GCAGCAGCTT 
TGTACTATCC TAATGGCTAC TGGGGTTCCT GCTGATATCC TGACTGAGAC CATAAATACT 
GTATCAGAAG TTATTCGAGG CTGCCAAGTA AACCAAGACT ACTTTGCATC TGTAAATGCA 
CCTTCAAACC CACCAAGACC GGCAATTGTA GTACTTCTCA TGTCCATGGT TAATGAAAGG 
CAGCCATTTG TTTTGCGCTG TGCTGTTCTC TATTGTTTCC AGTGTTTCTT GTATAAAAAC 
CAAAAAGGAC AAGGAGAAAT CGTGTCAACA CTTTTACCTT CTACCATTGA TGCAACAGGT 
AATTCAGTTT CAGCTGGCCA GTTATTATGT GGAGGTTTGT TTTCTACTGA TTCACTTTCA 
AACTGGTGTG CTGCTGTGGC CCTTGCCCAT GCGTTGCAAG AAAATGCCAC CCAGAAAGAA 
CAGTTGCTCA GGGTTCAACT TGCTACAAGT ATTGGCAACC CTCCAGTTTC TTTACTTCAA 
CAGTGCACCA ATATTCTTTC ACAGGGAAGC AAAATACAAA CAAGAGTTGG ATTATTAATG 
TTGCTTTGTA CCTGGCTAAG CAATTGTCCC ATTGCAGTAA CGCATTTTCT TCACAATTCA 
GCCAATGTTC CATTTCTTAC AGGACAAATT GCAGAAAATC TTGGAGAAGA AGAGCAGTTG 
GTCCAAGGCT TATGTGCCCT TTTGTTGGGC ATTTCGATTT ATTTCAATGA TAACTCACTT 
GAGAGCTACA TGAAAGAGAA GCTAAAACAA CTGATTGAGA AGAGGATTGG CAAAGAGAAT 
TTCATAGAGA AACTAGGATT TATTAGCAAA CATGAGTTGT ATTCCAGAGC ATCTCAGAAA 
CCCCAGCCAA ACTTTCCCAG TCCAGAATAC ATGATATTTG ATCATGAGTT TACGAAGCTG 
GTAAAAGAAC TTGAAGGTGT TATAACTAAG GCTATTTATA AGTCCAGTGA AGAAGATAAA 
AAAGAAGAAG AGGTGAAAAA AACATTAGAA CAGCATGACA ATATTGTGAC TCACTACAAA 
AATATGATTC GAGAGCAGGA TCTCCAACTT GAGGAATTAA GGCAGCAGGT TTCTACATTA 
AAATGTCAAA ATGAACAGCT CCAGACGGCA GTCACACAGC AAGTATCACA GATCCAGCAG 
CACAAAGACC AGTATAATCT TCTTAAAATA CAGCTAGGAA AAGACAATCA GCATCAAGGT 
TCTTACAGTG AGGGGGCTCA GATGAATGGC ATTCAGCCAG AAGAAATTGG TAGATTGCGA 
GAAGAGATAG AAGAATTAAA ACGTAATCAG GAACTTTTAC AAAGCCAGCT GACTGAAAAG 
GACTCTATGA TTGAAAATAT GAAATCTTCC CAAACATCTG GCACAAATGA ACAGTCTTCA 
GCAATAGTTT CAGCTAGAGA TTCTGAACAA GTTGCAGAAT TAAAACAGGA ACTGGCAACT 
TTAAAGTCTC AGTTAAACTC ACAATCTGTG GAGATCACCA AACTACAGAC AGAAAAGCAG 
GAACTGTTAC AGAAAACAGA AGCGTTTGCA AAATCAGTTG AGGTACAAGG AGAGACCGAG 
ACTATAATAG CCACCAAAAC TACTGATGTA GAAGGAAGAC TGTCAGCATT ATTACAAGAG 
ACCAAAGAGT TAAAGAATGA AATTAAAGCT CTGTCTGAGG AAAGAACTGC CATTAAAGAG 
CAGCTGGATT CATCTAATAG TACCATTGCC ATTTTACAAA CTGAGAAAGA CAAACTAGAG 
TTGGAAATTA CAGATTCTAA AAAAGAACAA GATGATCTCT TGGTGCTCTT GGCCGATCAA 
GATCAGAAAA TACTGTCATT GAAGAATAAA CTCAAGGATC TTGGTCATCC AGTTGAAGAA 
GAGGATGAAC TTGAATCTGG AGACCAAGAG GATGAGGATG ATGAAAGTGA AGATCCTGGC 
AAGGATCTAG ATCATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714460.1
CDS	299..3199
Translation	MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVDDVEEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI

Target ORF information:

RefSeq Version	XM_006714397.3
Organism	Homo sapiens(human)
Definition	Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714397.3

ORF Insert Sequence:

ATGAATTTCC TCCGCGGGGT AATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCC 
GAGACGATTC AAAAGCTTTG TGACAGAGTA GCTTCATCTA CTTTATTGGA TGATCGAAGA 
AATGCTGTTC GTGCTCTCAA ATCATTATCT AAGAAATACC GCTTGGAAGT GGGTATACAA 
GCTATGGAAC ATCTTATTCA TGTTTTACAA ACAGATCGTT CAGATTCTGA AATAATAGGT 
TATGCTTTGG ACACACTATA TAATATAATA TCTAATGAAG AAGAAGAAGA AGTAGATGAT 
GTTGAAGAAG AAAATTCCAC AAGACAGAGT GAAGATTTGG GAAGCCAATT TACAGAAATT 
TTCATTAAGC AGCAGGAAAA TGTCACTCTT CTGTTATCTT TATTGGAGGA GTTTGATTTC 
CATGTCCGCT GGCCTGGTGT GAAGCTTCTT ACTTCTCTTT TAAAACAACT AGGGCCTCAG 
GTGCAACAAA TTATTTTAGT CAGTCCTATG GGTGTTTCAA GATTGATGGA CTTACTAGCG 
GATTCCAGGG AAGTTATACG TAATGATGGC GTCTTACTAC TGCAGGCACT AACAAGAAGC 
AATGGTGCAA TCCAGAAAAT TGTTGCTTTT GAAAATGCTT TCGAGAGACT ACTGGACATT 
ATTTCAGAGG AGGGGAACAG TGATGGAGGT ATAGTAGTTG AAGATTGTTT GATTTTGCTC 
CAAAACTTAT TAAAAAACAA CAACTCCAAT CAAAATTTTT TTAAAGAAGG CTCATATATT 
CAACGTATGA AACCTTGGTT TGAAGTTGGA GATGAAAATT CTGGCTGGTC TGCACAGAAA 
GTGACCAATC TACATCTAAT GCTACAGCTT GTTCGTGTAT TGGTATCTCC CACCAACCCT 
CCTGGTGCTA CCAGTAGCTG CCAGAAGGCT ATGTTCCAGT GTGGGTTATT GCAGCAGCTT 
TGTACTATCC TAATGGCTAC TGGGGTTCCT GCTGATATCC TGACTGAGAC CATAAATACT 
GTATCAGAAG TTATTCGAGG CTGCCAAGTA AACCAAGACT ACTTTGCATC TGTAAATGCA 
CCTTCAAACC CACCAAGACC GGCAATTGTA GTACTTCTCA TGTCCATGGT TAATGAAAGG 
CAGCCATTTG TTTTGCGCTG TGCTGTTCTC TATTGTTTCC AGTGTTTCTT GTATAAAAAC 
CAAAAAGGAC AAGGAGAAAT CGTGTCAACA CTTTTACCTT CTACCATTGA TGCAACAGGT 
AATTCAGTTT CAGCTGGCCA GTTATTATGT GGAGGTTTGT TTTCTACTGA TTCACTTTCA 
AACTGGTGTG CTGCTGTGGC CCTTGCCCAT GCGTTGCAAG AAAATGCCAC CCAGAAAGAA 
CAGTTGCTCA GGGTTCAACT TGCTACAAGT ATTGGCAACC CTCCAGTTTC TTTACTTCAA 
CAGTGCACCA ATATTCTTTC ACAGGGAAGC AAAATACAAA CAAGAGTTGG ATTATTAATG 
TTGCTTTGTA CCTGGCTAAG CAATTGTCCC ATTGCAGTAA CGCATTTTCT TCACAATTCA 
GCCAATGTTC CATTTCTTAC AGGACAAATT GCAGAAAATC TTGGAGAAGA AGAGCAGTTG 
GTCCAAGGCT TATGTGCCCT TTTGTTGGGC ATTTCGATTT ATTTCAATGA TAACTCACTT 
GAGAGCTACA TGAAAGAGAA GCTAAAACAA CTGATTGAGA AGAGGATTGG CAAAGAGAAT 
TTCATAGAGA AACTAGGATT TATTAGCAAA CATGAGTTGT ATTCCAGAGC ATCTCAGAAA 
CCCCAGCCAA ACTTTCCCAG TCCAGAATAC ATGATATTTG ATCATGAGTT TACGAAGCTG 
GTAAAAGAAC TTGAAGGTGT TATAACTAAG GCTATTTATA AGTCCAGTGA AGAAGATAAA 
AAAGAAGAAG AGGTGAAAAA AACATTAGAA CAGCATGACA ATATTGTGAC TCACTACAAA 
AATATGATTC GAGAGCAGGA TCTCCAACTT GAGGAATTAA GGCAGCAGGT TTCTACATTA 
AAATGTCAAA ATGAACAGCT CCAGACGGCA GTCACACAGC AAGTATCACA GATCCAGCAG 
CACAAAGACC AGTATAATCT TCTTAAAATA CAGCTAGGAA AAGACAATCA GCATCAAGGT 
TCTTACAGTG AGGGGGCTCA GATGAATGGC ATTCAGCCAG AAGAAATTGG TAGATTGCGA 
GAAGAGATAG AAGAATTAAA ACGTAATCAG GAACTTTTAC AAAGCCAGCT GACTGAAAAG 
GACTCTATGA TTGAAAATAT GAAATCTTCC CAAACATCTG GCACAAATGA ACAGTCTTCA 
GCAATAGTTT CAGCTAGAGA TTCTGAACAA GTTGCAGAAT TAAAACAGGA ACTGGCAACT 
TTAAAGTCTC AGTTAAACTC ACAATCTGTG GAGATCACCA AACTACAGAC AGAAAAGCAG 
GAACTGTTAC AGAAAACAGA AGCGTTTGCA AAATCAGTTG AGGTACAAGG AGAGACCGAG 
ACTATAATAG CCACCAAAAC TACTGATGTA GAAGGAAGAC TGTCAGCATT ATTACAAGAG 
ACCAAAGAGT TAAAGAATGA AATTAAAGCT CTGTCTGAGG AAAGAACTGC CATTAAAGAG 
CAGCTGGATT CATCTAATAG TACCATTGCC ATTTTACAAA CTGAGAAAGA CAAACTAGAG 
TTGGAAATTA CAGATTCTAA AAAAGAACAA GATGATCTCT TGGTGCTCTT GGCCGATCAA 
GATCAGAAAA TACTGTCATT GAAGAATAAA CTCAAGGATC TTGGTCATCC AGTTGAAGAA 
GAGGATGAAC TTGAATCTGG AGACCAAGAG GATGAGGATG ATGAAAGTGA AGATCCTGGC 
AAGGATCTAG ATCATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie78264-71(2016 Mar)
Jin Y,Dai Z

Lentivirus-mediated silencing of USO1 inhibits cell proliferation and migration of human colon cancer cells.
Medical oncology (Northwood, London, England)32(8)218(2015 Aug)
Sui J,Li X,Xing J,Cao F,Wang H,Gong H,Zhang W

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

P115 promotes growth of gastric cancer through interaction with macrophage migration inhibitory factor.
World journal of gastroenterology19(46)8619-29(2013 Dec)
Li XJ,Luo Y,Yi YF

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

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USO1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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