MADD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MADD
Entrez Gene ID	8567
Full Name	MAP kinase activating death domain
Synonyms	DENN,IG20,RAB3GEP
General protein information	Preferred Names MAP kinase activating death domain Names MAP kinase-activating death domain protein Rab3 GDP/GTP exchange factor differentially expressed in normal and neoplastic cells insulinoma glucagonoma clone 20
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p11.2
Summary	Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	603584

mRNA and Protein(s)

mRNA	Protein	Name
XM_017018494.1	XP_016873983.1	MAP kinase-activating death domain protein isoform X21
XM_017018495.1	XP_016873984.1	MAP kinase-activating death domain protein isoform X22
XM_017018504.1	XP_016873993.1	MAP kinase-activating death domain protein isoform X28
XM_017018510.1	XP_016873999.1	MAP kinase-activating death domain protein isoform X33
NM_130471.2	NP_569827.2	MAP kinase-activating death domain protein isoform b
NM_130476.2	NP_569832.2	MAP kinase-activating death domain protein isoform h
XM_005253199.3	XP_005253256.1	MAP kinase-activating death domain protein isoform X15
XM_024448755.1	XP_024304523.1	MAP kinase-activating death domain protein isoform X31
XM_017018499.1	XP_016873988.1	MAP kinase-activating death domain protein isoform X32
XM_024448746.1	XP_024304514.1	MAP kinase-activating death domain protein isoform X2
XM_005253196.3	XP_005253253.1	MAP kinase-activating death domain protein isoform X7
XM_024448748.1	XP_024304516.1	MAP kinase-activating death domain protein isoform X9
XM_024448749.1	XP_024304517.1	MAP kinase-activating death domain protein isoform X10
XM_024448751.1	XP_024304519.1	MAP kinase-activating death domain protein isoform X24
NM_001376655.1	NP_001363584.1	MAP kinase-activating death domain protein isoform 67
NM_001376638.1	NP_001363567.1	MAP kinase-activating death domain protein isoform 53
NM_001376594.1	NP_001363523.1	MAP kinase-activating death domain protein isoform 24
NM_001376605.1	NP_001363534.1	MAP kinase-activating death domain protein isoform 30
NM_130474.3	NP_569830.2	MAP kinase-activating death domain protein isoform 6
NM_001376613.1	NP_001363542.1	MAP kinase-activating death domain protein isoform 38
NM_001376593.1	NP_001363522.1	MAP kinase-activating death domain protein isoform 24
NM_001376607.1	NP_001363536.1	MAP kinase-activating death domain protein isoform 32
NM_001376659.1	NP_001363588.1	MAP kinase-activating death domain protein isoform 70
NM_001376600.1	NP_001363529.1	MAP kinase-activating death domain protein isoform 27
NM_001376579.1	NP_001363508.1	MAP kinase-activating death domain protein isoform 5
NM_001376644.1	NP_001363573.1	MAP kinase-activating death domain protein isoform 57
NM_001376616.1	NP_001363545.1	MAP kinase-activating death domain protein isoform 3
NM_001376621.1	NP_001363550.1	MAP kinase-activating death domain protein isoform 10
NM_001376662.1	NP_001363591.1	MAP kinase-activating death domain protein isoform 73
NM_001376619.1	NP_001363548.1	MAP kinase-activating death domain protein isoform 10
NM_001376657.1	NP_001363586.1	MAP kinase-activating death domain protein isoform 68
XM_017018488.1	XP_016873977.1	MAP kinase-activating death domain protein isoform X20
XM_024448752.1	XP_024304520.1	MAP kinase-activating death domain protein isoform X25
XM_017018493.1	XP_016873982.1	MAP kinase-activating death domain protein isoform X27
XM_005253204.3	XP_005253261.1	MAP kinase-activating death domain protein isoform X29
XM_017018505.2	XP_016873994.1	MAP kinase-activating death domain protein isoform X34
XM_024448747.1	XP_024304515.1	MAP kinase-activating death domain protein isoform X4
XM_017018478.2	XP_016873967.1	MAP kinase-activating death domain protein isoform X5
XM_017018485.2	XP_016873974.1	MAP kinase-activating death domain protein isoform X16
NM_003682.3	NP_003673.3	MAP kinase-activating death domain protein isoform d
XM_017018480.1	XP_016873969.1	MAP kinase-activating death domain protein isoform X5
XM_005253201.2	XP_005253258.1	MAP kinase-activating death domain protein isoform X15
XM_005253204.2	XP_005253261.1	MAP kinase-activating death domain protein isoform X23
XM_017018479.1	XP_016873968.1	MAP kinase-activating death domain protein isoform X3
XM_017018482.1	XP_016873971.1	MAP kinase-activating death domain protein isoform X7
XM_005253199.2	XP_005253256.1	MAP kinase-activating death domain protein isoform X9
XM_017018484.1	XP_016873973.1	MAP kinase-activating death domain protein isoform X10
XM_017018508.1	XP_016873997.1	MAP kinase-activating death domain protein isoform X31
XM_005253205.1	XP_005253262.1	MAP kinase-activating death domain protein isoform X34
XM_017018503.1	XP_016873992.1	MAP kinase-activating death domain protein isoform X28
XM_017018502.1	XP_016873991.1	MAP kinase-activating death domain protein isoform X27
NM_130474.2	NP_569830.2	MAP kinase-activating death domain protein isoform f
NM_130475.2	NP_569831.1	MAP kinase-activating death domain protein isoform g
NM_001376609.1	NP_001363538.1	MAP kinase-activating death domain protein isoform 34
NM_001376622.1	NP_001363551.1	MAP kinase-activating death domain protein isoform 41
NM_001376624.1	NP_001363553.1	MAP kinase-activating death domain protein isoform 42
NM_130470.3	NP_569826.2	MAP kinase-activating death domain protein isoform 1
NM_001376636.1	NP_001363565.1	MAP kinase-activating death domain protein isoform 52
NM_001376654.1	NP_001363583.1	MAP kinase-activating death domain protein isoform 66
NM_001376629.1	NP_001363558.1	MAP kinase-activating death domain protein isoform 46
NM_001376646.1	NP_001363575.1	MAP kinase-activating death domain protein isoform 59
NM_001376625.1	NP_001363554.1	MAP kinase-activating death domain protein isoform 42
NM_001376663.1	NP_001363592.1	MAP kinase-activating death domain protein isoform 74
NM_001376627.1	NP_001363556.1	MAP kinase-activating death domain protein isoform 45
NM_130473.3	NP_569829.2	MAP kinase-activating death domain protein isoform 5
NM_001376611.1	NP_001363540.1	MAP kinase-activating death domain protein isoform 36
NM_001376643.1	NP_001363572.1	MAP kinase-activating death domain protein isoform 56
NM_001376653.1	NP_001363582.1	MAP kinase-activating death domain protein isoform 65
NM_001376634.1	NP_001363563.1	MAP kinase-activating death domain protein isoform 50
NM_001376612.1	NP_001363541.1	MAP kinase-activating death domain protein isoform 37
XM_005253205.2	XP_005253262.1	MAP kinase-activating death domain protein isoform X36
NM_003682.4	NP_003673.3	MAP kinase-activating death domain protein isoform 4
XM_017018509.1	XP_016873998.1	MAP kinase-activating death domain protein isoform X32
XM_005253196.2	XP_005253253.1	MAP kinase-activating death domain protein isoform X4
XM_017018483.1	XP_016873972.1	MAP kinase-activating death domain protein isoform X8
XM_017018485.1	XP_016873974.1	MAP kinase-activating death domain protein isoform X10
XM_017018487.1	XP_016873976.1	MAP kinase-activating death domain protein isoform X12
XM_017018491.1	XP_016873980.1	MAP kinase-activating death domain protein isoform X17
XM_017018478.1	XP_016873967.1	MAP kinase-activating death domain protein isoform X2
XM_024448750.1	XP_024304518.1	MAP kinase-activating death domain protein isoform X13
XM_017018489.1	XP_016873978.1	MAP kinase-activating death domain protein isoform X20
XM_005253203.3	XP_005253260.1	MAP kinase-activating death domain protein isoform X28
XM_024448753.1	XP_024304521.1	MAP kinase-activating death domain protein isoform X30
XM_024448754.1	XP_024304522.1	MAP kinase-activating death domain protein isoform X30
NM_130470.2	NP_569826.2	MAP kinase-activating death domain protein isoform a
NM_001135943.1	NP_001129415.1	MAP kinase-activating death domain protein isoform i
NM_001376574.1	NP_001363503.1	MAP kinase-activating death domain protein isoform 14
NM_001376639.1	NP_001363568.1	MAP kinase-activating death domain protein isoform 53
NM_001376583.1	NP_001363512.1	MAP kinase-activating death domain protein isoform 21
NM_001376575.1	NP_001363504.1	MAP kinase-activating death domain protein isoform 15
NM_130471.3	NP_569827.2	MAP kinase-activating death domain protein isoform 2
NM_001376597.1	NP_001363526.1	MAP kinase-activating death domain protein isoform 25
NM_001376632.1	NP_001363561.1	MAP kinase-activating death domain protein isoform 48
NM_001376650.1	NP_001363579.1	MAP kinase-activating death domain protein isoform 63
NM_001376620.1	NP_001363549.1	MAP kinase-activating death domain protein isoform 40
NM_001376577.1	NP_001363506.1	MAP kinase-activating death domain protein isoform 16
NM_001376586.1	NP_001363515.1	MAP kinase-activating death domain protein isoform 23
NM_001376602.1	NP_001363531.1	MAP kinase-activating death domain protein isoform 28
NM_001376628.1	NP_001363557.1	MAP kinase-activating death domain protein isoform 44
NM_001376642.1	NP_001363571.1	MAP kinase-activating death domain protein isoform 56

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MADD	NP_001180007.1
Rattus norvegicus (Norway rat)	Madd	NP_446037.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000630	XP_311160.5
Caenorhabditis elegans (roundworm)	aex-3	NP_741710.1
Xenopus tropicalis (tropical clawed frog)	madd	NP_001072717.1
Mus musculus (house mouse)	Madd	NP_001171191.1
Danio rerio (zebrafish)	madd	XP_005169012.1
	MADD	XP_001107689.1
Homo sapiens (human)	MADD	NP_003673.3
Pan troglodytes (chimpanzee)	MADD	XP_003951918.1
Canis lupus familiaris (dog)	MADD	XP_005631300.1
Gallus gallus (chicken)	MADD	XP_004941623.1
Drosophila melanogaster (fruit fly)	rab3-GEF	NP_001096983.2

Gene Ontology
Bibliography

Related articles in PubMed

MADD-FOLH1 Polymorphisms and Their Haplotypes with Serum Lipid Levels and the Risk of Coronary Heart Disease and Ischemic Stroke in a Chinese Han Population.
Wu DF, Yin RX, Cao XL, Huang F, Wu JZ, Chen WX
Nutrients8(4)208(2016 Apr)

Epigenetic silencing of tumor suppressor miR-3151 contributes to Chinese chronic lymphocytic leukemia by constitutive activation of MADD/ERK and PIK3R2/AKT signaling pathways.
Wang LQ, Wong KY, Ros?n A, Chim CS
Oncotarget6(42)44422-36(2015 Dec)

MADD is a downstream target of PTEN in triggering apoptosis.
Jayarama S, Li LC, Ganesh L, Mardi D, Kanteti P, Hay N, Li P, Prabhakar BS
Journal of cellular biochemistry115(2)261-70(2014 Feb)

MADD promotes the survival of human lung adenocarcinoma cells by inhibiting apoptosis.
Bi W, Wei Y, Wu J, Sun G, Guo Y, Zhang Q, Dong L
Oncology reports29(4)1533-9(2013 Apr)

MADD knock-down enhances doxorubicin and TRAIL induced apoptosis in breast cancer cells.
Turner A, Li LC, Pilli T, Qian L, Wiley EL, Setty S, Christov K, Ganesh L, Maker AV, Li P, Kanteti P, Das Gupta TK, Prabhakar BS
PloS one8(2)e56817(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The risk of coronary disease and ischemic stroke associated with multiple polymorphisms and haplotypes of MADD and FOLH1 in Han Chinese patients are reported in association with alcohol consumption.
Title: MADD-FOLH1 Polymorphisms and Their Haplotypes with Serum Lipid Levels and the Risk of Coronary Heart Disease and Ischemic Stroke in a Chinese Han Population.

miR-3151 silencing by DNA methylation protected chronic lymphocytic leukemia cells from apoptosis by over-expression of its direct targets MADD and PIK3R2, constitutive activation of MEK/ERK and PI3K/AKT signaling , and over-expression of MCL1.
Title: Epigenetic silencing of tumor suppressor miR-3151 contributes to Chinese chronic lymphocytic leukemia by constitutive activation of MADD/ERK and PIK3R2/AKT signaling pathways.

Taken together, our data reveal that PTEN can convey the death signal by preventing MADD phosphorylation by Akt.
Title: MADD is a downstream target of PTEN in triggering apoptosis.

These results suggest that MADD may be a potential therapeutic target for lung adenocarcinoma therapy involving the TRAIL-induced apoptosis pathway
Title: MADD promotes the survival of human lung adenocarcinoma cells by inhibiting apoptosis.

MADD knockdown resulted in enhanced spontaneous apoptosis in human breast cancer cell lines
Title: MADD knock-down enhances doxorubicin and TRAIL induced apoptosis in breast cancer cells.

The following MADD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MADD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu109167	NM_001376654.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 88, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu109170	NM_001376659.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 93, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu109181	NM_001376663.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 97, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu109193	NM_001376662.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 96, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu109198	NM_001376657.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 91, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu109201	NM_001376655.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 89, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu92648	NM_001376653.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 87, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu92648	XM_017018509.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu92649	XM_017018510.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu103652	NM_001376624.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 58, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu103652	NM_001376625.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 59, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu103653	NM_001376628.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 61, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109144	NM_001376583.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109151	NM_001376597.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109152	NM_001376613.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 47, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109154	NM_001376632.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 66, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109161	NM_001376650.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 84, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109163	NM_001376620.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 54, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109164	NM_001376577.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109169	NM_001376607.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109172	NM_001376646.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 80, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109173	NM_001376586.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109175	NM_001376579.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109176	NM_001376602.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109183	NM_001376627.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 62, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109185	NM_001376644.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 78, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109190	NM_001376611.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109194	NM_001376634.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 68, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu109197	NM_001376612.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 46, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu45059	NM_001376574.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu45060	NM_001376575.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu45075	NM_001376616.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 50, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu45076	NM_001376621.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 55, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu45076	NM_001376619.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 53, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92630	NM_001376593.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92630	NM_001376594.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92633	NM_001376600.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92634	NM_001376605.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92636	NM_001376609.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92640	NM_001376622.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 56, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92641	NM_001376629.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 63, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92643	NM_001376636.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 70, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92644	NM_001376638.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 72, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92644	NM_001376639.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 73, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92645	NM_001376642.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 76, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92645	NM_001376643.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 77, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu103649	XM_024448749.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu103650	XM_024448750.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu103651	XM_024448752.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu103652	XM_024448753.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu103652	XM_024448754.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu103653	XM_024448755.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu45056	XM_024448746.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu45058	XM_024448747.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu45073	XM_024448751.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu61992	XM_024448748.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92624	XM_017018478.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92625	XM_017018479.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92626	XM_017018480.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92628	XM_017018482.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92629	XM_017018483.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92630	XM_017018484.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92630	XM_017018485.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92632	XM_017018487.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92633	XM_017018488.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92633	XM_017018489.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92635	XM_017018491.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92637	XM_017018493.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92638	XM_017018494.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92639	XM_017018495.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92641	XM_017018499.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92643	XM_017018502.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92644	XM_017018504.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92644	XM_017018503.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92645	XM_017018505.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92647	XM_017018508.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu05881	NM_130470.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu05883	NM_130471.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu05887	NM_130474.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu18382	NM_003682.3 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu30554	NM_130476.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu31353	NM_130475.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu32000	NM_001135943.1 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45057	NM_003682.4 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45066	NM_130473.3 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45070	NM_130470.3 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45072	NM_130471.3 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45077	NM_130474.3 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu45066	XM_005253196.3 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45070	XM_005253199.3 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45072	XM_005253201.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45075	XM_005253203.3 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45076	XM_005253204.3 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45077	XM_005253205.2 Latest version!	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu45076	XM_005253204.2	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45070	XM_005253199.2	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45077	XM_005253205.1	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu45066	XM_005253196.2	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu92630	XM_017018485.1	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu92624	XM_017018478.1	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MADD ( NM_001376654.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363583.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 88, mRNA.

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CloneID	OHu109167
Clone ID Related Accession (Same CDS sequence)	NM_001376654.1
Accession Version	NM_001376654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 66
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2372367.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGGG CCTGAAGTAA TCAAACCTGT CTTTGACCTT 
GGTGAGACAG AGGAGAAAAA GTCCCAGATC AGCGCAGACA GTGGTGTGAG CCTGACGTCT 
AGTTCCCAGA GGACTGATCA AGACTCTGTC ATCGGCGTGA GTCCAGCTGT TATGATCCGC 
AGCTCAAGTC AGGATTCTGA AGTTAGCACC GTGGTGAGTA ATAGCTCTGG AGAGACCCTT 
GGAGCTGACA GTGACTTGAG CAGCAATGCA GGTGATGGAC CAGGTGGCGA GGGCAGTGTT 
CACCTGGCAA GCTCTCGGGG CACTTTGTCT GATAGTGAAA TTGAGACCAA CTCTGCCACA 
AGCACCATCT TTGGTAAAGC CCACAGCTTG AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC 
AGCCCCATTC GTACTTCTGA AGATGTGAGC CAGCGAGTCT ATCTCTATGA GGGACTCCTA 
GGCAAAGAGC GTTCTACTTT ATGGGACCAA ATGCAATTCT GGGAAGATGC CTTCTTAGAT 
GCTGTGATGT TGGAGAGAGA AGGGATGGGT ATGGACCAGG GTCCCCAGGA AATGATCGAC 
AGGTACCTGT CCCTTGGAGA ACATGACCGG AAGCGCCTGG AAGATGATGA AGATCGCTTG 
TTGGCCACAC TTCTGCACAA CCTCATCTCC TACATGCTGC TGATGAAGGT AAATAAGAAT 
GACATCCGCA AGAAGGTGAG GCGCCTAATG GGAAAGTCGC ACATTGGGCT TGTGTACAGC 
CAGCAAATCA ATGAGGTGCT TGATCAGCTG GCGAACCTGA ATGGACGCGA TCTCTCTATC 
TGGTCCAGTG GCAGCCGGCA CATGAAGAAG CAGACATTTG TGGTACATGC AGGGACAGAT 
ACAAACGGAG ATATCTTTTT CATGGAGGTG TGCGATGACT GTGTGGTGTT GCGTAGTAAC 
ATCGGAACAG TGTATGAGCG CTGGTGGTAC GAGAAGCTCA TCAACATGAC CTACTGTCCC 
AAGACGAAGG TGTTGTGCTT GTGGCGTAGA AATGGCTCTG AGACCCAGCT CAACAAGTTC 
TATACTAAAA AGTGTCGGGA GCTGTACTAC TGTGTGAAGG ACAGCATGGA GCGCGCTGCC 
GCCCGACAGC AAAGCATCAA ACCCGGACCT GAATTGGGTG GCGAGTTCCC TGTGCAGGAC 
CTGAAGACTG GTGAGGGTGG CCTGCTGCAG GTGACCCTGG AAGGGATCAA CCTCAAATTC 
ATGCACAATC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTTCGAGGC 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ATTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACACCAATGG CCCACGAAAT CTGCTACTCC GTATTATGTC TCTTCTCGTA CGTGGCTGCA 
GTTCATAGCA GTGAGGAAGA TCTCAGAACC CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363583.1
CDS	257..4750
Translation	MSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376654.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 88, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376654.1

ORF Insert Sequence:

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGGG CCTGAAGTAA TCAAACCTGT CTTTGACCTT 
GGTGAGACAG AGGAGAAAAA GTCCCAGATC AGCGCAGACA GTGGTGTGAG CCTGACGTCT 
AGTTCCCAGA GGACTGATCA AGACTCTGTC ATCGGCGTGA GTCCAGCTGT TATGATCCGC 
AGCTCAAGTC AGGATTCTGA AGTTAGCACC GTGGTGAGTA ATAGCTCTGG AGAGACCCTT 
GGAGCTGACA GTGACTTGAG CAGCAATGCA GGTGATGGAC CAGGTGGCGA GGGCAGTGTT 
CACCTGGCAA GCTCTCGGGG CACTTTGTCT GATAGTGAAA TTGAGACCAA CTCTGCCACA 
AGCACCATCT TTGGTAAAGC CCACAGCTTG AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC 
AGCCCCATTC GTACTTCTGA AGATGTGAGC CAGCGAGTCT ATCTCTATGA GGGACTCCTA 
GGCAAAGAGC GTTCTACTTT ATGGGACCAA ATGCAATTCT GGGAAGATGC CTTCTTAGAT 
GCTGTGATGT TGGAGAGAGA AGGGATGGGT ATGGACCAGG GTCCCCAGGA AATGATCGAC 
AGGTACCTGT CCCTTGGAGA ACATGACCGG AAGCGCCTGG AAGATGATGA AGATCGCTTG 
TTGGCCACAC TTCTGCACAA CCTCATCTCC TACATGCTGC TGATGAAGGT AAATAAGAAT 
GACATCCGCA AGAAGGTGAG GCGCCTAATG GGAAAGTCGC ACATTGGGCT TGTGTACAGC 
CAGCAAATCA ATGAGGTGCT TGATCAGCTG GCGAACCTGA ATGGACGCGA TCTCTCTATC 
TGGTCCAGTG GCAGCCGGCA CATGAAGAAG CAGACATTTG TGGTACATGC AGGGACAGAT 
ACAAACGGAG ATATCTTTTT CATGGAGGTG TGCGATGACT GTGTGGTGTT GCGTAGTAAC 
ATCGGAACAG TGTATGAGCG CTGGTGGTAC GAGAAGCTCA TCAACATGAC CTACTGTCCC 
AAGACGAAGG TGTTGTGCTT GTGGCGTAGA AATGGCTCTG AGACCCAGCT CAACAAGTTC 
TATACTAAAA AGTGTCGGGA GCTGTACTAC TGTGTGAAGG ACAGCATGGA GCGCGCTGCC 
GCCCGACAGC AAAGCATCAA ACCCGGACCT GAATTGGGTG GCGAGTTCCC TGTGCAGGAC 
CTGAAGACTG GTGAGGGTGG CCTGCTGCAG GTGACCCTGG AAGGGATCAA CCTCAAATTC 
ATGCACAATC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTTCGAGGC 
GTCTTTGTCC TGGAGGAATT TGTTCCTGAA ATTAAAGAAG TGGTGAGCCA CAAGTACAAG 
ACACCAATGG CCCACGAAAT CTGCTACTCC GTATTATGTC TCTTCTCGTA CGTGGCTGCA 
GTTCATAGCA GTGAGGAAGA TCTCAGAACC CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376659.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363588.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 93, mRNA.

Price & Availability↑

CloneID	OHu109170
Clone ID Related Accession (Same CDS sequence)	NM_001376659.1
Accession Version	NM_001376659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 70
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.160723.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTAATCAAAC CTGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC GTCTAGTTCC 
CAGAGGACTG ATCAAGACTC TGTCATCGGC GTGAGTCCAG CTGTTATGAT CCGCAGCTCA 
AGTCAGGATT CTGAAGTGAG TAATAGCTCT GGAGAGACCC TTGGAGCTGA CAGTGACTTG 
AGCAGCAATG CAGGTGATGG ACCAGGTGGC GAGGGCAGTG TTCACCTGGC AAGCTCTCGG 
GGCACTTTGT CTGATAGTGA AATTGAGACC AACTCTGCCA CAAGCACCAT CTTTGGTAAA 
GCCCACAGCT TGAAGCCAAG CATAAAGGAG AAGCTGGCAG GCAGCCCCAT TCGTACTTCT 
GAAGATGTGA GCCAGCGAGT CTATCTCTAT GAGGGACTCC TAGGAAGGGA CAAAGGATCC 
ATGTGGGACC AGTTAGAGGA TGCAGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT 
ACTTTATGGG ACCAAATGCA ATTCTGGGAA GATGCCTTCT TAGATGCTGT GATGTTGGAG 
AGAGAAGGGA TGGGTATGGA CCAGGGTCCC CAGGAAATGA TCGACAGGTA CCTGTCCCTT 
GGAGAACATG ACCGGAAGCG CCTGGAAGAT GATGAAGATC GCTTGTTGGC CACACTTCTG 
CACAACCTCA TCTCCTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGCAAGAAG 
GTGAGGCGCC TAATGGGAAA GTCGCACATT GGGCTTGTGT ACAGCCAGCA AATCAATGAG 
GTGCTTGATC AGCTGGCGAA CCTGAATGGA CGCGATCTCT CTATCTGGTC CAGTGGCAGC 
CGGCACATGA AGAAGCAGAC ATTTGTGGTA CATGCAGGGA CAGATACAAA CGGAGATATC 
TTTTTCATGG AGGTGTGCGA TGACTGTGTG GTGTTGCGTA GTAACATCGG AACAGTGTAT 
GAGCGCTGGT GGTACGAGAA GCTCATCAAC ATGACCTACT GTCCCAAGAC GAAGGTGTTG 
TGCTTGTGGC GTAGAAATGG CTCTGAGACC CAGCTCAACA AGTTCTATAC TAAAAAGTGT 
CGGGAGCTGT ACTACTGTGT GAAGGACAGC ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC 
ATCAAACCCG GACCTGAATT GGGTGGCGAG TTCCCTGTGC AGGACCTGAA GACTGGTGAG 
GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG ATCAACCTCA AATTCATGCA CAATCAGTTC 
CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363588.1
CDS	257..4657
Translation	MSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376659.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 93, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376659.1

ORF Insert Sequence:

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTAATCAAAC CTGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC GTCTAGTTCC 
CAGAGGACTG ATCAAGACTC TGTCATCGGC GTGAGTCCAG CTGTTATGAT CCGCAGCTCA 
AGTCAGGATT CTGAAGTGAG TAATAGCTCT GGAGAGACCC TTGGAGCTGA CAGTGACTTG 
AGCAGCAATG CAGGTGATGG ACCAGGTGGC GAGGGCAGTG TTCACCTGGC AAGCTCTCGG 
GGCACTTTGT CTGATAGTGA AATTGAGACC AACTCTGCCA CAAGCACCAT CTTTGGTAAA 
GCCCACAGCT TGAAGCCAAG CATAAAGGAG AAGCTGGCAG GCAGCCCCAT TCGTACTTCT 
GAAGATGTGA GCCAGCGAGT CTATCTCTAT GAGGGACTCC TAGGAAGGGA CAAAGGATCC 
ATGTGGGACC AGTTAGAGGA TGCAGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT 
ACTTTATGGG ACCAAATGCA ATTCTGGGAA GATGCCTTCT TAGATGCTGT GATGTTGGAG 
AGAGAAGGGA TGGGTATGGA CCAGGGTCCC CAGGAAATGA TCGACAGGTA CCTGTCCCTT 
GGAGAACATG ACCGGAAGCG CCTGGAAGAT GATGAAGATC GCTTGTTGGC CACACTTCTG 
CACAACCTCA TCTCCTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGCAAGAAG 
GTGAGGCGCC TAATGGGAAA GTCGCACATT GGGCTTGTGT ACAGCCAGCA AATCAATGAG 
GTGCTTGATC AGCTGGCGAA CCTGAATGGA CGCGATCTCT CTATCTGGTC CAGTGGCAGC 
CGGCACATGA AGAAGCAGAC ATTTGTGGTA CATGCAGGGA CAGATACAAA CGGAGATATC 
TTTTTCATGG AGGTGTGCGA TGACTGTGTG GTGTTGCGTA GTAACATCGG AACAGTGTAT 
GAGCGCTGGT GGTACGAGAA GCTCATCAAC ATGACCTACT GTCCCAAGAC GAAGGTGTTG 
TGCTTGTGGC GTAGAAATGG CTCTGAGACC CAGCTCAACA AGTTCTATAC TAAAAAGTGT 
CGGGAGCTGT ACTACTGTGT GAAGGACAGC ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC 
ATCAAACCCG GACCTGAATT GGGTGGCGAG TTCCCTGTGC AGGACCTGAA GACTGGTGAG 
GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG ATCAACCTCA AATTCATGCA CAATCAGTTC 
CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376663.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363592.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 97, mRNA.

Price & Availability↑

CloneID	OHu109181
Clone ID Related Accession (Same CDS sequence)	NM_001376663.1
Accession Version	NM_001376663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 74
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.110028.1 [ECO:0000332] ##Evidence-Data-END##

ATGTGGCGGA TCTTTACTGG ATCGCTGCTG GTAGAGGAGA AGTCAAGTGC CCTTCTGCAT 
GACCTTCGAG AGATTGAGGC CTGGATCTAT CGATTGCTGC GCTCCCCAGT ACCCGTCTCT 
GGGCAGAAGC GAGTAGACAT CGAGGTCCTA CCCCAAGAGC TCCAGCCAGC TCTGACCTTT 
GCTCTTCCAG ACCCATCTCG ATTCACCCTA GTGGATTTCC CACTGCACCT TCCCTTGGAA 
CTTCTAGGTG TGGACGCCTG TCTCCAGGTG CTAACCTGCA TTCTGTTAGA GCACAAGGTG 
GTGCTACAGT CCCGAGACTA CAATGCACTC TCCATGTCTG TGATGGCATT CGTGGCAATG 
ATCTACCCAC TGGAGTATAT GTTTCCTGTC ATCCCGCTGC TACCCACCTG CATGGCATCA 
GCAGAGCAGC TGCTGTTGGC TCCAACCCCG TACATCATTG GGGTTCCTGC CAGCTTCTTC 
CTCTACAAAC TGGACTTCAA AATGCCTGAT GATGTATGGC TAGTGGATCT GGACAGCAAT 
AGGGTGATTG CCCCCACCAA TGCAGAAGTG CTGCCTATCC TGCCAGAACC AGAATCACTA 
GAGCTGAAAA AGCATTTAAA GCAGGCCTTG GCCAGCATGA GTCTCAACAC CCAGCCCATC 
CTCAATCTGG AGAAATTTCA TGAGGGCCAG GAGATCCCCC TTCTCTTGGG AAGGCCTTCT 
AATGACCTGC AGTCCACACC GTCCACTGAA TTCAACCCAC TCATCTATGG CAATGATGTG 
GATTCTGTGG ATGTTGCAAC CAGGGTTGCC ATGGTACGGT TCTTCAATTC CGCCAACGTG 
CTGCAGGGAT TTCAGATGCA CACGCGTACC CTGCGCCTCT TTCCTCGGCC TGTGGTAGCT 
TTTCAAGCTG GCTCCTTTCT AGCCTCACGT CCCCGGCAGA CTCCTTTTGC CGAGAAATTG 
GCCAGGACTC AGGCTGTGGA GTACTTTGGG GAATGGATCC TTAACCCCAC CAACTATGCC 
TTTCAGCGAA TTCACAACAA TATGTTTGAT CCAGCCCTGA TTGGTGACAA GCCAAAGTGG 
TATGCTCATC AGCTGCAGCC TATCCACTAT CGCGTCTATG ACAGCAATTC CCAGCTGGCT 
GAGGCCCTGA GTGTACCACC AGAGCGGGAC TCTGACTCCG AACCTACTGA TGATAGTGGC 
AGTGATAGTA TGGATTATGA CGATTCAAGC TCTTCTTACT CCTCCCTTGG TGACTTTGTC 
AGTGAAATGA TGAAATGTGA CATTAATGGT GATACTCCCA ATGTGGACCC TCTGACACAT 
GCAGCACTGG GGGATGCCAG CGAGGTGGAG ATTGACGAGC TGCAGAATCA GAAGGAAGCA 
GAAGAGCCTG GCCCAGACAG TGAGAACTCT CAGGAAAACC CCCCACTGCG CTCCAGCTCT 
AGCACCACAG CCAGCAGCAG CCCCAGCACT GTCATCCACG GAGCCAACTC TGAACCTGCT 
GACTCTACGG AGATGGATGA TAAGGCAGCA GTAGGCGTCT CCAAGCCCCT CCCTTCCGTG 
CCTCCCAGCA TTGGCAAATC GAACGTGGAC AGACGTCAGG CAGAAATTGG AGAGGGGTCA 
GTGCGCCGGC GAATCTATGA CAATCCATAC TTCGAGCCCC AATATGGCTT TCCCCCTGAG 
GAAGATGAGG ATGAGCAGGG GGAAAGTTAC ACTCCCCGAT TCAGCCAACA TGTCAGTGGC 
AATCGGGCTC AAAAGCTGCT GCGGCCCAAC AGCTTGAGAC TGGCAAGTGA CTCAGATGCA 
GAGTCAGACT CTCGGGCAAG CTCTCCCAAC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTCTATCGGA ACCACAGTAC CAGCTTCAGT 
CTTTCAAACC TCACACTGCC CACCAAAGGT GCCCGAGAGA AGGCCACGCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC GTTAGTGGAT CAGAAGTCAT CTGTCATTAA ACACAGCCCA 
ACAGTGAAAA GAGAACCTCC ATCACCCCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAGGTGGT GCACAGCGTG CTGGACGGCC AGGGAGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGCCGGCT GCTGGAGAGC GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGCA TGGTGCAGTC AGAGGACGAT GCCCGGCAGG ACATCATCCC GGATGTGGAG 
ATCAGTCGGA AGGTGTACAA GGGAATGTTA GACCTCCTCA AGTGTACAGT CCTCAGCTTG 
GAGCAGTCCT ATGCCCACGC GGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTTTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAA 
AGTGTAAATA CCCCAGTTGG CAAGGATCCT GGCCTAGCTG GGCGGGGGGA CCCAAAGGCT 
ATGGCACAAC TGAGAGTTCC ACAACTGGGA CCTCGGGCAC CAAGTGCCAC AGGAAAGGGT 
CCTAAGGAAC TGGACACCAG AAGTTTAAAG GAAGAAAATT TTATAGCATC TATTGGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ACGAAATCTG CTACTCCGTA 
TTATGTCTCT TCTCGTACGT GGCTGCAGTT CATAGCAGTG AGGAAGATCT CAGAACCCCG 
CCCCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363592.1
CDS	122..4222
Translation	MWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376663.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 97, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376663.1

ORF Insert Sequence:

ATGTGGCGGA TCTTTACTGG ATCGCTGCTG GTAGAGGAGA AGTCAAGTGC CCTTCTGCAT 
GACCTTCGAG AGATTGAGGC CTGGATCTAT CGATTGCTGC GCTCCCCAGT ACCCGTCTCT 
GGGCAGAAGC GAGTAGACAT CGAGGTCCTA CCCCAAGAGC TCCAGCCAGC TCTGACCTTT 
GCTCTTCCAG ACCCATCTCG ATTCACCCTA GTGGATTTCC CACTGCACCT TCCCTTGGAA 
CTTCTAGGTG TGGACGCCTG TCTCCAGGTG CTAACCTGCA TTCTGTTAGA GCACAAGGTG 
GTGCTACAGT CCCGAGACTA CAATGCACTC TCCATGTCTG TGATGGCATT CGTGGCAATG 
ATCTACCCAC TGGAGTATAT GTTTCCTGTC ATCCCGCTGC TACCCACCTG CATGGCATCA 
GCAGAGCAGC TGCTGTTGGC TCCAACCCCG TACATCATTG GGGTTCCTGC CAGCTTCTTC 
CTCTACAAAC TGGACTTCAA AATGCCTGAT GATGTATGGC TAGTGGATCT GGACAGCAAT 
AGGGTGATTG CCCCCACCAA TGCAGAAGTG CTGCCTATCC TGCCAGAACC AGAATCACTA 
GAGCTGAAAA AGCATTTAAA GCAGGCCTTG GCCAGCATGA GTCTCAACAC CCAGCCCATC 
CTCAATCTGG AGAAATTTCA TGAGGGCCAG GAGATCCCCC TTCTCTTGGG AAGGCCTTCT 
AATGACCTGC AGTCCACACC GTCCACTGAA TTCAACCCAC TCATCTATGG CAATGATGTG 
GATTCTGTGG ATGTTGCAAC CAGGGTTGCC ATGGTACGGT TCTTCAATTC CGCCAACGTG 
CTGCAGGGAT TTCAGATGCA CACGCGTACC CTGCGCCTCT TTCCTCGGCC TGTGGTAGCT 
TTTCAAGCTG GCTCCTTTCT AGCCTCACGT CCCCGGCAGA CTCCTTTTGC CGAGAAATTG 
GCCAGGACTC AGGCTGTGGA GTACTTTGGG GAATGGATCC TTAACCCCAC CAACTATGCC 
TTTCAGCGAA TTCACAACAA TATGTTTGAT CCAGCCCTGA TTGGTGACAA GCCAAAGTGG 
TATGCTCATC AGCTGCAGCC TATCCACTAT CGCGTCTATG ACAGCAATTC CCAGCTGGCT 
GAGGCCCTGA GTGTACCACC AGAGCGGGAC TCTGACTCCG AACCTACTGA TGATAGTGGC 
AGTGATAGTA TGGATTATGA CGATTCAAGC TCTTCTTACT CCTCCCTTGG TGACTTTGTC 
AGTGAAATGA TGAAATGTGA CATTAATGGT GATACTCCCA ATGTGGACCC TCTGACACAT 
GCAGCACTGG GGGATGCCAG CGAGGTGGAG ATTGACGAGC TGCAGAATCA GAAGGAAGCA 
GAAGAGCCTG GCCCAGACAG TGAGAACTCT CAGGAAAACC CCCCACTGCG CTCCAGCTCT 
AGCACCACAG CCAGCAGCAG CCCCAGCACT GTCATCCACG GAGCCAACTC TGAACCTGCT 
GACTCTACGG AGATGGATGA TAAGGCAGCA GTAGGCGTCT CCAAGCCCCT CCCTTCCGTG 
CCTCCCAGCA TTGGCAAATC GAACGTGGAC AGACGTCAGG CAGAAATTGG AGAGGGGTCA 
GTGCGCCGGC GAATCTATGA CAATCCATAC TTCGAGCCCC AATATGGCTT TCCCCCTGAG 
GAAGATGAGG ATGAGCAGGG GGAAAGTTAC ACTCCCCGAT TCAGCCAACA TGTCAGTGGC 
AATCGGGCTC AAAAGCTGCT GCGGCCCAAC AGCTTGAGAC TGGCAAGTGA CTCAGATGCA 
GAGTCAGACT CTCGGGCAAG CTCTCCCAAC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTCTATCGGA ACCACAGTAC CAGCTTCAGT 
CTTTCAAACC TCACACTGCC CACCAAAGGT GCCCGAGAGA AGGCCACGCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC GTTAGTGGAT CAGAAGTCAT CTGTCATTAA ACACAGCCCA 
ACAGTGAAAA GAGAACCTCC ATCACCCCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAGGTGGT GCACAGCGTG CTGGACGGCC AGGGAGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGCCGGCT GCTGGAGAGC GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGCA TGGTGCAGTC AGAGGACGAT GCCCGGCAGG ACATCATCCC GGATGTGGAG 
ATCAGTCGGA AGGTGTACAA GGGAATGTTA GACCTCCTCA AGTGTACAGT CCTCAGCTTG 
GAGCAGTCCT ATGCCCACGC GGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTTTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAA 
AGTGTAAATA CCCCAGTTGG CAAGGATCCT GGCCTAGCTG GGCGGGGGGA CCCAAAGGCT 
ATGGCACAAC TGAGAGTTCC ACAACTGGGA CCTCGGGCAC CAAGTGCCAC AGGAAAGGGT 
CCTAAGGAAC TGGACACCAG AAGTTTAAAG GAAGAAAATT TTATAGCATC TATTGGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ACGAAATCTG CTACTCCGTA 
TTATGTCTCT TCTCGTACGT GGCTGCAGTT CATAGCAGTG AGGAAGATCT CAGAACCCCG 
CCCCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376662.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363591.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 96, mRNA.

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CloneID	OHu109193
Clone ID Related Accession (Same CDS sequence)	NM_001376662.1
Accession Version	NM_001376662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 73
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.101554.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGAACCAG ACAAGCGGAA GAGAAGTCCA ACAGAAAGTG TAAATACCCC AGTTGGCAAG 
GATCCTGGCC TAGCTGGGCG GGGGGACCCA AAGGCTATGG CACAACTGAG AGTTCCACAA 
CTGGGACCTC GGGCACCAAG TGCCACAGGA AAGGGTCCTA AGGAACTGGA CACCAGAAGT 
TTAAAGGAAG AAAATTTTAT AGCATCTATT GAATTGTGGA ACAAGCACCA GGAAGTGAAA 
AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT GAAGTAATCA AACCTGTCTT TGACCTTGGT 
GAGACAGAGG AGAAAAAGTC CCAGATCAGC GCAGACAGTG GTGTGAGCCT GACGTCTAGT 
TCCCAGAGGA CTGATCAAGA CTCTGTCATC GGCGTGAGTC CAGCTGTTAT GATCCGCAGC 
TCAAGTCAGG ATTCTGAAGT TAGCACCGTG AGTAATAGCT CTGGAGAGAC CCTTGGAGCT 
GACAGTGACT TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GCGAGGGCAG TGTTCACCTG 
GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAACTCTGC CACAAGCACC 
ATCTTTGGTA AAGCCCACAG CTTGAAGCCA AGCATAAAGG AGAAGCTGGC AGGCAGCCCC 
ATTCGTACTT CTGAAGATGT GAGCCAGCGA GTCTATCTCT ATGAGGGACT CCTAGGAAGG 
GACAAAGGAT CCATGTGGGA CCAGTTAGAG GATGCAGCTA TGGAGACCTT TTCTATAAGC 
AAAGAGCGTT CTACTTTATG GGACCAAATG CAATTCTGGG AAGATGCCTT CTTAGATGCT 
GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG 
TACCTGTCCC TTGGAGAACA TGACCGGAAG CGCCTGGAAG ATGATGAAGA TCGCTTGTTG 
GCCACACTTC TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC 
ATCCGCAAGA AGGTGAGGCG CCTAATGGGA AAGTCGCACA TTGGGCTTGT GTACAGCCAG 
CAAATCAATG AGGTGCTTGA TCAGCTGGCG AACCTGAATG GACGCGATCT CTCTATCTGG 
TCCAGTGGCA GCCGGCACAT GAAGAAGCAG ACATTTGTGG TACATGCAGG GACAGATACA 
AACGGAGATA TCTTTTTCAT GGAGGTGTGC GATGACTGTG TGGTGTTGCG TAGTAACATC 
GGAACAGTGT ATGAGCGCTG GTGGTACGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG 
ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT 
ACTAAAAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG CGCTGCCGCC 
CGACAGCAAA GCATCAAACC CGGACCTGAA TTGGGTGGCG AGTTCCCTGT GCAGGACCTG 
AAGACTGGTG AGGGTGGCCT GCTGCAGGTG ACCCTGGAAG GGATCAACCT CAAATTCATG 
CACAATCAGT TCCTGAAATT AAAGAAGTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363591.1
CDS	203..4315
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376662.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 96, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376662.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGAACCAG ACAAGCGGAA GAGAAGTCCA ACAGAAAGTG TAAATACCCC AGTTGGCAAG 
GATCCTGGCC TAGCTGGGCG GGGGGACCCA AAGGCTATGG CACAACTGAG AGTTCCACAA 
CTGGGACCTC GGGCACCAAG TGCCACAGGA AAGGGTCCTA AGGAACTGGA CACCAGAAGT 
TTAAAGGAAG AAAATTTTAT AGCATCTATT GAATTGTGGA ACAAGCACCA GGAAGTGAAA 
AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT GAAGTAATCA AACCTGTCTT TGACCTTGGT 
GAGACAGAGG AGAAAAAGTC CCAGATCAGC GCAGACAGTG GTGTGAGCCT GACGTCTAGT 
TCCCAGAGGA CTGATCAAGA CTCTGTCATC GGCGTGAGTC CAGCTGTTAT GATCCGCAGC 
TCAAGTCAGG ATTCTGAAGT TAGCACCGTG AGTAATAGCT CTGGAGAGAC CCTTGGAGCT 
GACAGTGACT TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GCGAGGGCAG TGTTCACCTG 
GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAACTCTGC CACAAGCACC 
ATCTTTGGTA AAGCCCACAG CTTGAAGCCA AGCATAAAGG AGAAGCTGGC AGGCAGCCCC 
ATTCGTACTT CTGAAGATGT GAGCCAGCGA GTCTATCTCT ATGAGGGACT CCTAGGAAGG 
GACAAAGGAT CCATGTGGGA CCAGTTAGAG GATGCAGCTA TGGAGACCTT TTCTATAAGC 
AAAGAGCGTT CTACTTTATG GGACCAAATG CAATTCTGGG AAGATGCCTT CTTAGATGCT 
GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG 
TACCTGTCCC TTGGAGAACA TGACCGGAAG CGCCTGGAAG ATGATGAAGA TCGCTTGTTG 
GCCACACTTC TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC 
ATCCGCAAGA AGGTGAGGCG CCTAATGGGA AAGTCGCACA TTGGGCTTGT GTACAGCCAG 
CAAATCAATG AGGTGCTTGA TCAGCTGGCG AACCTGAATG GACGCGATCT CTCTATCTGG 
TCCAGTGGCA GCCGGCACAT GAAGAAGCAG ACATTTGTGG TACATGCAGG GACAGATACA 
AACGGAGATA TCTTTTTCAT GGAGGTGTGC GATGACTGTG TGGTGTTGCG TAGTAACATC 
GGAACAGTGT ATGAGCGCTG GTGGTACGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG 
ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT 
ACTAAAAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG CGCTGCCGCC 
CGACAGCAAA GCATCAAACC CGGACCTGAA TTGGGTGGCG AGTTCCCTGT GCAGGACCTG 
AAGACTGGTG AGGGTGGCCT GCTGCAGGTG ACCCTGGAAG GGATCAACCT CAAATTCATG 
CACAATCAGT TCCTGAAATT AAAGAAGTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376657.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363586.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 91, mRNA.

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CloneID	OHu109198
Clone ID Related Accession (Same CDS sequence)	NM_001376657.1
Accession Version	NM_001376657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 68
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.173064.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGT CAGTGCGCCG GCGAATCTAT 
GACAATCCAT ACTTCGAGCC CCAATATGGC TTTCCCCCTG AGGAAGATGA GGATGAGCAG 
GGGGAAAGTT ACACTCCCCG ATTCAGCCAA CATGTCAGTG GCAATCGGGC TCAAAAGCTG 
CTGCGGCCCA ACAGCTTGAG ACTGGCAAGT GACTCAGATG CAGAGTCAGA CTCTCGGGCA 
AGCTCTCCCA ACTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT 
TTTGCCAGCA GCCTCTATCG GAACCACAGT ACCAGCTTCA GTCTTTCAAA CCTCACACTG 
CCCACCAAAG GTGCCCGAGA GAAGGCCACG CCCTTCCCCA GTCTGAAAGG AAACAGGAGG 
GCGTTAGTGG ATCAGAAGTC ATCTGTCATT AAACACAGCC CAACAGTGAA AAGAGAACCT 
CCATCACCCC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAGGTG 
GTGCACAGCG TGCTGGACGG CCAGGGAGTT GGCTGGCTCA ACATGAAAAA GGTGCGCCGG 
CTGCTGGAGA GCGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG CATGGTGCAG 
TCAGAGGACG ATGCCCGGCA GGACATCATC CCGGATGTGG AGATCAGTCG GAAGGTGTAC 
AAGGGAATGT TAGACCTCCT CAAGTGTACA GTCCTCAGCT TGGAGCAGTC CTATGCCCAC 
GCGGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTTTGG AGATTGCCCA GACCCACTAC 
TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AAAGTGTAAA TACCCCAGTT 
GGCAAGGATC CTGGCCTAGC TGGGCGGGGG GACCCAAAGG CTATGGCACA ACTGAGAGTT 
CCACAACTGG GACCTCGGGC ACCAAGTGCC ACAGGAAAGG GTCCTAAGGA ACTGGACACC 
AGAAGTTTAA AGGAAGAAAA TTTTATAGCA TCTATTGAAT TGTGGAACAA GCACCAGGAA 
GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TAATCAAACC TGTCTTTGAC 
CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACG 
TCTAGTTCCC AGAGGACTGA TCAAGACTCT GTCATCGGCG TGAGTCCAGC TGTTATGATC 
CGCAGCTCAA GTCAGGATTC TGAAGTGAGT AATAGCTCTG GAGAGACCCT TGGAGCTGAC 
AGTGACTTGA GCAGCAATGC AGGTGATGGA CCAGGTGGCG AGGGCAGTGT TCACCTGGCA 
AGCTCTCGGG GCACTTTGTC TGATAGTGAA ATTGAGACCA ACTCTGCCAC AAGCACCATC 
TTTGGTAAAG CCCACAGCTT GAAGCCAAGC ATAAAGGAGA AGCTGGCAGG CAGCCCCATT 
CGTACTTCTG AAGATGTGAG CCAGCGAGTC TATCTCTATG AGGGACTCCT AGGAAGGGAC 
AAAGGATCCA TGTGGGACCA GTTAGAGGAT GCAGCTATGG AGACCTTTTC TATAAGCAAA 
GAGCGTTCTA CTTTATGGGA CCAAATGCAA TTCTGGGAAG ATGCCTTCTT AGATGCTGTG 
ATGTTGGAGA GAGAAGGGAT GGGTATGGAC CAGGGTCCCC AGGAAATGAT CGACAGGTAC 
CTGTCCCTTG GAGAACATGA CCGGAAGCGC CTGGAAGATG ATGAAGATCG CTTGTTGGCC 
ACACTTCTGC ACAACCTCAT CTCCTACATG CTGCTGATGA AGGTAAATAA GAATGACATC 
CGCAAGAAGG TGAGGCGCCT AATGGGAAAG TCGCACATTG GGCTTGTGTA CAGCCAGCAA 
ATCAATGAGG TGCTTGATCA GCTGGCGAAC CTGAATGGAC GCGATCTCTC TATCTGGTCC 
AGTGGCAGCC GGCACATGAA GAAGCAGACA TTTGTGGTAC ATGCAGGGAC AGATACAAAC 
GGAGATATCT TTTTCATGGA GGTGTGCGAT GACTGTGTGG TGTTGCGTAG TAACATCGGA 
ACAGTGTATG AGCGCTGGTG GTACGAGAAG CTCATCAACA TGACCTACTG TCCCAAGACG 
AAGGTGTTGT GCTTGTGGCG TAGAAATGGC TCTGAGACCC AGCTCAACAA GTTCTATACT 
AAAAAGTGTC GGGAGCTGTA CTACTGTGTG AAGGACAGCA TGGAGCGCGC TGCCGCCCGA 
CAGCAAAGCA TCAAACCCGG ACCTGAATTG GGTGGCGAGT TCCCTGTGCA GGACCTGAAG 
ACTGGTGAGG GTGGCCTGCT GCAGGTGACC CTGGAAGGGA TCAACCTCAA ATTCATGCAC 
AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363586.1
CDS	257..4726
Translation	MSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376657.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 91, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376657.1

ORF Insert Sequence:

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGT CAGTGCGCCG GCGAATCTAT 
GACAATCCAT ACTTCGAGCC CCAATATGGC TTTCCCCCTG AGGAAGATGA GGATGAGCAG 
GGGGAAAGTT ACACTCCCCG ATTCAGCCAA CATGTCAGTG GCAATCGGGC TCAAAAGCTG 
CTGCGGCCCA ACAGCTTGAG ACTGGCAAGT GACTCAGATG CAGAGTCAGA CTCTCGGGCA 
AGCTCTCCCA ACTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT 
TTTGCCAGCA GCCTCTATCG GAACCACAGT ACCAGCTTCA GTCTTTCAAA CCTCACACTG 
CCCACCAAAG GTGCCCGAGA GAAGGCCACG CCCTTCCCCA GTCTGAAAGG AAACAGGAGG 
GCGTTAGTGG ATCAGAAGTC ATCTGTCATT AAACACAGCC CAACAGTGAA AAGAGAACCT 
CCATCACCCC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAGGTG 
GTGCACAGCG TGCTGGACGG CCAGGGAGTT GGCTGGCTCA ACATGAAAAA GGTGCGCCGG 
CTGCTGGAGA GCGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG CATGGTGCAG 
TCAGAGGACG ATGCCCGGCA GGACATCATC CCGGATGTGG AGATCAGTCG GAAGGTGTAC 
AAGGGAATGT TAGACCTCCT CAAGTGTACA GTCCTCAGCT TGGAGCAGTC CTATGCCCAC 
GCGGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTTTGG AGATTGCCCA GACCCACTAC 
TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AAAGTGTAAA TACCCCAGTT 
GGCAAGGATC CTGGCCTAGC TGGGCGGGGG GACCCAAAGG CTATGGCACA ACTGAGAGTT 
CCACAACTGG GACCTCGGGC ACCAAGTGCC ACAGGAAAGG GTCCTAAGGA ACTGGACACC 
AGAAGTTTAA AGGAAGAAAA TTTTATAGCA TCTATTGAAT TGTGGAACAA GCACCAGGAA 
GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TAATCAAACC TGTCTTTGAC 
CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACG 
TCTAGTTCCC AGAGGACTGA TCAAGACTCT GTCATCGGCG TGAGTCCAGC TGTTATGATC 
CGCAGCTCAA GTCAGGATTC TGAAGTGAGT AATAGCTCTG GAGAGACCCT TGGAGCTGAC 
AGTGACTTGA GCAGCAATGC AGGTGATGGA CCAGGTGGCG AGGGCAGTGT TCACCTGGCA 
AGCTCTCGGG GCACTTTGTC TGATAGTGAA ATTGAGACCA ACTCTGCCAC AAGCACCATC 
TTTGGTAAAG CCCACAGCTT GAAGCCAAGC ATAAAGGAGA AGCTGGCAGG CAGCCCCATT 
CGTACTTCTG AAGATGTGAG CCAGCGAGTC TATCTCTATG AGGGACTCCT AGGAAGGGAC 
AAAGGATCCA TGTGGGACCA GTTAGAGGAT GCAGCTATGG AGACCTTTTC TATAAGCAAA 
GAGCGTTCTA CTTTATGGGA CCAAATGCAA TTCTGGGAAG ATGCCTTCTT AGATGCTGTG 
ATGTTGGAGA GAGAAGGGAT GGGTATGGAC CAGGGTCCCC AGGAAATGAT CGACAGGTAC 
CTGTCCCTTG GAGAACATGA CCGGAAGCGC CTGGAAGATG ATGAAGATCG CTTGTTGGCC 
ACACTTCTGC ACAACCTCAT CTCCTACATG CTGCTGATGA AGGTAAATAA GAATGACATC 
CGCAAGAAGG TGAGGCGCCT AATGGGAAAG TCGCACATTG GGCTTGTGTA CAGCCAGCAA 
ATCAATGAGG TGCTTGATCA GCTGGCGAAC CTGAATGGAC GCGATCTCTC TATCTGGTCC 
AGTGGCAGCC GGCACATGAA GAAGCAGACA TTTGTGGTAC ATGCAGGGAC AGATACAAAC 
GGAGATATCT TTTTCATGGA GGTGTGCGAT GACTGTGTGG TGTTGCGTAG TAACATCGGA 
ACAGTGTATG AGCGCTGGTG GTACGAGAAG CTCATCAACA TGACCTACTG TCCCAAGACG 
AAGGTGTTGT GCTTGTGGCG TAGAAATGGC TCTGAGACCC AGCTCAACAA GTTCTATACT 
AAAAAGTGTC GGGAGCTGTA CTACTGTGTG AAGGACAGCA TGGAGCGCGC TGCCGCCCGA 
CAGCAAAGCA TCAAACCCGG ACCTGAATTG GGTGGCGAGT TCCCTGTGCA GGACCTGAAG 
ACTGGTGAGG GTGGCCTGCT GCAGGTGACC CTGGAAGGGA TCAACCTCAA ATTCATGCAC 
AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376655.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363584.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 89, mRNA.

Price & Availability↑

CloneID	OHu109201
Clone ID Related Accession (Same CDS sequence)	NM_001376655.1
Accession Version	NM_001376655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 67
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.165324.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363584.1
CDS	203..4693
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376655.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 89, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376655.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376653.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363582.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 87, mRNA.

Price & Availability↑

CloneID	OHu92648
Clone ID Related Accession (Same CDS sequence)	NM_001376653.1 , XM_017018509.1
Accession Version	NM_001376653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 65
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.6227.1, SRR1660805.217546.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363582.1
CDS	203..4702
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376653.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 87, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376653.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018509.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873998.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X39, mRNA.

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CloneID	OHu92648
Clone ID Related Accession (Same CDS sequence)	NM_001376653.1 , XM_017018509.1
Accession Version	XM_017018509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873998.1
CDS	172..4671
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018509.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018509.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018510.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873999.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X40, mRNA.

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CloneID	OHu92649
Clone ID Related Accession (Same CDS sequence)	XM_017018510.1
Accession Version	XM_017018510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873999.1
CDS	216..4706
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018510.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018510.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376624.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363553.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 58, mRNA.

Price & Availability↑

CloneID	OHu103652
Clone ID Related Accession (Same CDS sequence)	NM_001376624.1 , NM_001376625.1 , XM_024448753.1 , XM_024448754.1
Accession Version	NM_001376624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 42
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_024448753.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.69033.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363553.1
CDS	203..4819
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376624.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 58, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376624.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376625.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363554.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 59, mRNA.

Price & Availability↑

CloneID	OHu103652
Clone ID Related Accession (Same CDS sequence)	NM_001376624.1 , NM_001376625.1 , XM_024448753.1 , XM_024448754.1
Accession Version	NM_001376625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 42
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_024448754.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.68176.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363554.1
CDS	333..4949
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376625.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 59, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376625.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376628.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363557.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 61, mRNA.

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CloneID	OHu103653
Clone ID Related Accession (Same CDS sequence)	XM_024448755.1 , NM_001376628.1
Accession Version	NM_001376628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 44
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_024448755.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.242989.1, SRR1803617.166529.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363557.1
CDS	203..4816
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376628.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 61, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376628.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376583.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363512.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 23, mRNA.

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CloneID	OHu109144
Clone ID Related Accession (Same CDS sequence)	NM_001376583.1
Accession Version	NM_001376583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.141413.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGAAACAGGA GGGCGTTAGT GGATCAGAAG 
TCATCTGTCA TTAAACACAG CCCAACAGTG AAAAGAGAAC CTCCATCACC CCAGGGTCGA 
TCCAGCAATT CTAGTGAGAA CCAGCAGTTC CTGAAGGAGG TGGTGCACAG CGTGCTGGAC 
GGCCAGGGAG TTGGCTGGCT CAACATGAAA AAGGTGCGCC GGCTGCTGGA GAGCGAGCAG 
CTGCGAGTCT TTGTCCTGAG CAAGCTGAAC CGCATGGTGC AGTCAGAGGA CGATGCCCGG 
CAGGACATCA TCCCGGATGT GGAGATCAGT CGGAAGGTGT ACAAGGGAAT GTTAGACCTC 
CTCAAGTGTA CAGTCCTCAG CTTGGAGCAG TCCTATGCCC ACGCGGGTCT GGGTGGCATG 
GCCAGCATCT TTGGGCTTTT GGAGATTGCC CAGACCCACT ACTATAGTAA AGAACCAGAC 
AAGCGGAAGA GAAGTCCAAC AGAAAGTGTA AATACCCCAG TTGGCAAGGA TCCTGGCCTA 
GCTGGGCGGG GGGACCCAAA GGCTATGGCA CAACTGAGAG TTCCACAACT GGGACCTCGG 
GCACCAAGTG CCACAGGAAA GGGTCCTAAG GAACTGGACA CCAGAAGTTT AAAGGAAGAA 
AATTTTATAG CATCTATTGA ATTGTGGAAC AAGCACCAGG AAGTGAAAAA GCAAAAAGCT 
TTGGAAAAAC AGAGGCCTGA AGTAATCAAA CCTGTCTTTG ACCTTGGTGA GACAGAGGAG 
AAAAAGTCCC AGATCAGCGC AGACAGTGGT GTGAGCCTGA CGTCTAGTTC CCAGAGGACT 
GATCAAGACT CTGTCATCGG CGTGAGTCCA GCTGTTATGA TCCGCAGCTC AAGTCAGGAT 
TCTGAAGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGAAGGG ACAAAGGATC CATGTGGGAC 
CAGTTAGAGG ATGCAGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AATTCTGGGA AGATGCCTTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT TGGAGAACAT 
GACCGGAAGC GCCTGGAAGA TGATGAAGAT CGCTTGTTGG CCACACTTCT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGCAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCGCACAT TGGGCTTGTG TACAGCCAGC AAATCAATGA GGTGCTTGAT 
CAGCTGGCGA ACCTGAATGG ACGCGATCTC TCTATCTGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTTGTGGT ACATGCAGGG ACAGATACAA ACGGAGATAT CTTTTTCATG 
GAGGTGTGCG ATGACTGTGT GGTGTTGCGT AGTAACATCG GAACAGTGTA TGAGCGCTGG 
TGGTACGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CGAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC CCAGCTCAAC AAGTTCTATA CTAAAAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGC GCTGCCGCCC GACAGCAAAG CATCAAACCC 
GGACCTGAAT TGGGTGGCGA GTTCCCTGTG CAGGACCTGA AGACTGGTGA GGGTGGCCTG 
CTGCAGGTGA CCCTGGAAGG GATCAACCTC AAATTCATGC ACAATCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTTC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC AATGGCCCAC 
GAAATCTGCT ACTCCGTATT ATGTCTCTTC TCGTACGTGG CTGCAGTTCA TAGCAGTGAG 
GAAGATCTCA GAACCCCGCC CCGGCCTGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363512.1
CDS	203..4981
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFARNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376583.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376583.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGAAACAGGA GGGCGTTAGT GGATCAGAAG 
TCATCTGTCA TTAAACACAG CCCAACAGTG AAAAGAGAAC CTCCATCACC CCAGGGTCGA 
TCCAGCAATT CTAGTGAGAA CCAGCAGTTC CTGAAGGAGG TGGTGCACAG CGTGCTGGAC 
GGCCAGGGAG TTGGCTGGCT CAACATGAAA AAGGTGCGCC GGCTGCTGGA GAGCGAGCAG 
CTGCGAGTCT TTGTCCTGAG CAAGCTGAAC CGCATGGTGC AGTCAGAGGA CGATGCCCGG 
CAGGACATCA TCCCGGATGT GGAGATCAGT CGGAAGGTGT ACAAGGGAAT GTTAGACCTC 
CTCAAGTGTA CAGTCCTCAG CTTGGAGCAG TCCTATGCCC ACGCGGGTCT GGGTGGCATG 
GCCAGCATCT TTGGGCTTTT GGAGATTGCC CAGACCCACT ACTATAGTAA AGAACCAGAC 
AAGCGGAAGA GAAGTCCAAC AGAAAGTGTA AATACCCCAG TTGGCAAGGA TCCTGGCCTA 
GCTGGGCGGG GGGACCCAAA GGCTATGGCA CAACTGAGAG TTCCACAACT GGGACCTCGG 
GCACCAAGTG CCACAGGAAA GGGTCCTAAG GAACTGGACA CCAGAAGTTT AAAGGAAGAA 
AATTTTATAG CATCTATTGA ATTGTGGAAC AAGCACCAGG AAGTGAAAAA GCAAAAAGCT 
TTGGAAAAAC AGAGGCCTGA AGTAATCAAA CCTGTCTTTG ACCTTGGTGA GACAGAGGAG 
AAAAAGTCCC AGATCAGCGC AGACAGTGGT GTGAGCCTGA CGTCTAGTTC CCAGAGGACT 
GATCAAGACT CTGTCATCGG CGTGAGTCCA GCTGTTATGA TCCGCAGCTC AAGTCAGGAT 
TCTGAAGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGAAGGG ACAAAGGATC CATGTGGGAC 
CAGTTAGAGG ATGCAGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AATTCTGGGA AGATGCCTTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT TGGAGAACAT 
GACCGGAAGC GCCTGGAAGA TGATGAAGAT CGCTTGTTGG CCACACTTCT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGCAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCGCACAT TGGGCTTGTG TACAGCCAGC AAATCAATGA GGTGCTTGAT 
CAGCTGGCGA ACCTGAATGG ACGCGATCTC TCTATCTGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTTGTGGT ACATGCAGGG ACAGATACAA ACGGAGATAT CTTTTTCATG 
GAGGTGTGCG ATGACTGTGT GGTGTTGCGT AGTAACATCG GAACAGTGTA TGAGCGCTGG 
TGGTACGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CGAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC CCAGCTCAAC AAGTTCTATA CTAAAAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGC GCTGCCGCCC GACAGCAAAG CATCAAACCC 
GGACCTGAAT TGGGTGGCGA GTTCCCTGTG CAGGACCTGA AGACTGGTGA GGGTGGCCTG 
CTGCAGGTGA CCCTGGAAGG GATCAACCTC AAATTCATGC ACAATCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTTC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC AATGGCCCAC 
GAAATCTGCT ACTCCGTATT ATGTCTCTTC TCGTACGTGG CTGCAGTTCA TAGCAGTGAG 
GAAGATCTCA GAACCCCGCC CCGGCCTGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376597.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363526.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 31, mRNA.

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CloneID	OHu109151
Clone ID Related Accession (Same CDS sequence)	NM_001376597.1
Accession Version	NM_001376597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.228257.1, SRR1803614.188959.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCAATGGCC CACGAAATCT GCTACTCCGT ATTATGTCTC 
TTCTCGTACG TGGCTGCAGT TCATAGCAGT GAGGAAGATC TCAGAACCCC GCCCCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363526.1
CDS	203..4954
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376597.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376597.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCAATGGCC CACGAAATCT GCTACTCCGT ATTATGTCTC 
TTCTCGTACG TGGCTGCAGT TCATAGCAGT GAGGAAGATC TCAGAACCCC GCCCCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376613.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363542.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 47, mRNA.

Price & Availability↑

CloneID	OHu109152
Clone ID Related Accession (Same CDS sequence)	NM_001376613.1
Accession Version	NM_001376613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 38
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGAAACAGGA GGGCGTTAGT GGATCAGAAG 
TCATCTGTCA TTAAACACAG CCCAACAGTG AAAAGAGAAC CTCCATCACC CCAGGGTCGA 
TCCAGCAATT CTAGTGAGAA CCAGCAGTTC CTGAAGGAGG TGGTGCACAG CGTGCTGGAC 
GGCCAGGGAG TTGGCTGGCT CAACATGAAA AAGGTGCGCC GGCTGCTGGA GAGCGAGCAG 
CTGCGAGTCT TTGTCCTGAG CAAGCTGAAC CGCATGGTGC AGTCAGAGGA CGATGCCCGG 
CAGGACATCA TCCCGGATGT GGAGATCAGT CGGAAGGTGT ACAAGGGAAT GTTAGACCTC 
CTCAAGTGTA CAGTCCTCAG CTTGGAGCAG TCCTATGCCC ACGCGGGTCT GGGTGGCATG 
GCCAGCATCT TTGGGCTTTT GGAGATTGCC CAGACCCACT ACTATAGTAA AGAACCAGAC 
AAGCGGAAGA GAAGTCCAAC AGAAAGTGTA AATACCCCAG TTGGCAAGGA TCCTGGCCTA 
GCTGGGCGGG GGGACCCAAA GGCTATGGCA CAACTGAGAG TTCCACAACT GGGACCTCGG 
GCACCAAGTG CCACAGGAAA GGGTCCTAAG GAACTGGACA CCAGAAGTTT AAAGGAAGAA 
AATTTTATAG CATCTATTGG GCCTGAAGTA ATCAAACCTG TCTTTGACCT TGGTGAGACA 
GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC AGTGGTGTGA GCCTGACGTC TAGTTCCCAG 
AGGACTGATC AAGACTCTGT CATCGGCGTG AGTCCAGCTG TTATGATCCG CAGCTCAAGT 
CAGGATTCTG AAGTGAGTAA TAGCTCTGGA GAGACCCTTG GAGCTGACAG TGACTTGAGC 
AGCAATGCAG GTGATGGACC AGGTGGCGAG GGCAGTGTTC ACCTGGCAAG CTCTCGGGGC 
ACTTTGTCTG ATAGTGAAAT TGAGACCAAC TCTGCCACAA GCACCATCTT TGGTAAAGCC 
CACAGCTTGA AGCCAAGCAT AAAGGAGAAG CTGGCAGGCA GCCCCATTCG TACTTCTGAA 
GATGTGAGCC AGCGAGTCTA TCTCTATGAG GGACTCCTAG GCAAAGAGCG TTCTACTTTA 
TGGGACCAAA TGCAATTCTG GGAAGATGCC TTCTTAGATG CTGTGATGTT GGAGAGAGAA 
GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTTGGAGAA 
CATGACCGGA AGCGCCTGGA AGATGATGAA GATCGCTTGT TGGCCACACT TCTGCACAAC 
CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGCAA GAAGGTGAGG 
CGCCTAATGG GAAAGTCGCA CATTGGGCTT GTGTACAGCC AGCAAATCAA TGAGGTGCTT 
GATCAGCTGG CGAACCTGAA TGGACGCGAT CTCTCTATCT GGTCCAGTGG CAGCCGGCAC 
ATGAAGAAGC AGACATTTGT GGTACATGCA GGGACAGATA CAAACGGAGA TATCTTTTTC 
ATGGAGGTGT GCGATGACTG TGTGGTGTTG CGTAGTAACA TCGGAACAGT GTATGAGCGC 
TGGTGGTACG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACGAAGGT GTTGTGCTTG 
TGGCGTAGAA ATGGCTCTGA GACCCAGCTC AACAAGTTCT ATACTAAAAA GTGTCGGGAG 
CTGTACTACT GTGTGAAGGA CAGCATGGAG CGCGCTGCCG CCCGACAGCA AAGCATCAAA 
CCCGGACCTG AATTGGGTGG CGAGTTCCCT GTGCAGGACC TGAAGACTGG TGAGGGTGGC 
CTGCTGCAGG TGACCCTGGA AGGGATCAAC CTCAAATTCA TGCACAATCA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTTCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCACGAAATC 
TGCTACTCCG TATTATGTCT CTTCTCGTAC GTGGCTGCAG TTCATAGCAG TGAGGAAGAT 
CTCAGAACCC CGCCCCGGCC TGTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363542.1
CDS	203..4855
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFARNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376613.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 47, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376613.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGAAACAGGA GGGCGTTAGT GGATCAGAAG 
TCATCTGTCA TTAAACACAG CCCAACAGTG AAAAGAGAAC CTCCATCACC CCAGGGTCGA 
TCCAGCAATT CTAGTGAGAA CCAGCAGTTC CTGAAGGAGG TGGTGCACAG CGTGCTGGAC 
GGCCAGGGAG TTGGCTGGCT CAACATGAAA AAGGTGCGCC GGCTGCTGGA GAGCGAGCAG 
CTGCGAGTCT TTGTCCTGAG CAAGCTGAAC CGCATGGTGC AGTCAGAGGA CGATGCCCGG 
CAGGACATCA TCCCGGATGT GGAGATCAGT CGGAAGGTGT ACAAGGGAAT GTTAGACCTC 
CTCAAGTGTA CAGTCCTCAG CTTGGAGCAG TCCTATGCCC ACGCGGGTCT GGGTGGCATG 
GCCAGCATCT TTGGGCTTTT GGAGATTGCC CAGACCCACT ACTATAGTAA AGAACCAGAC 
AAGCGGAAGA GAAGTCCAAC AGAAAGTGTA AATACCCCAG TTGGCAAGGA TCCTGGCCTA 
GCTGGGCGGG GGGACCCAAA GGCTATGGCA CAACTGAGAG TTCCACAACT GGGACCTCGG 
GCACCAAGTG CCACAGGAAA GGGTCCTAAG GAACTGGACA CCAGAAGTTT AAAGGAAGAA 
AATTTTATAG CATCTATTGG GCCTGAAGTA ATCAAACCTG TCTTTGACCT TGGTGAGACA 
GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC AGTGGTGTGA GCCTGACGTC TAGTTCCCAG 
AGGACTGATC AAGACTCTGT CATCGGCGTG AGTCCAGCTG TTATGATCCG CAGCTCAAGT 
CAGGATTCTG AAGTGAGTAA TAGCTCTGGA GAGACCCTTG GAGCTGACAG TGACTTGAGC 
AGCAATGCAG GTGATGGACC AGGTGGCGAG GGCAGTGTTC ACCTGGCAAG CTCTCGGGGC 
ACTTTGTCTG ATAGTGAAAT TGAGACCAAC TCTGCCACAA GCACCATCTT TGGTAAAGCC 
CACAGCTTGA AGCCAAGCAT AAAGGAGAAG CTGGCAGGCA GCCCCATTCG TACTTCTGAA 
GATGTGAGCC AGCGAGTCTA TCTCTATGAG GGACTCCTAG GCAAAGAGCG TTCTACTTTA 
TGGGACCAAA TGCAATTCTG GGAAGATGCC TTCTTAGATG CTGTGATGTT GGAGAGAGAA 
GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTTGGAGAA 
CATGACCGGA AGCGCCTGGA AGATGATGAA GATCGCTTGT TGGCCACACT TCTGCACAAC 
CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGCAA GAAGGTGAGG 
CGCCTAATGG GAAAGTCGCA CATTGGGCTT GTGTACAGCC AGCAAATCAA TGAGGTGCTT 
GATCAGCTGG CGAACCTGAA TGGACGCGAT CTCTCTATCT GGTCCAGTGG CAGCCGGCAC 
ATGAAGAAGC AGACATTTGT GGTACATGCA GGGACAGATA CAAACGGAGA TATCTTTTTC 
ATGGAGGTGT GCGATGACTG TGTGGTGTTG CGTAGTAACA TCGGAACAGT GTATGAGCGC 
TGGTGGTACG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACGAAGGT GTTGTGCTTG 
TGGCGTAGAA ATGGCTCTGA GACCCAGCTC AACAAGTTCT ATACTAAAAA GTGTCGGGAG 
CTGTACTACT GTGTGAAGGA CAGCATGGAG CGCGCTGCCG CCCGACAGCA AAGCATCAAA 
CCCGGACCTG AATTGGGTGG CGAGTTCCCT GTGCAGGACC TGAAGACTGG TGAGGGTGGC 
CTGCTGCAGG TGACCCTGGA AGGGATCAAC CTCAAATTCA TGCACAATCA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTTCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCACGAAATC 
TGCTACTCCG TATTATGTCT CTTCTCGTAC GTGGCTGCAG TTCATAGCAG TGAGGAAGAT 
CTCAGAACCC CGCCCCGGCC TGTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376632.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363561.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 66, mRNA.

Price & Availability↑

CloneID	OHu109154
Clone ID Related Accession (Same CDS sequence)	NM_001376632.1
Accession Version	NM_001376632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 48
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.200427.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGCTTGAGAC TGGCAAGTGA CTCAGATGCA GAGTCAGACT CTCGGGCAAG CTCTCCCAAC 
TCCACCGTCT CCAACACCAG CACCGAGGGC TTCGGGGGCA TCATGTCTTT TGCCAGCAGC 
CTCTATCGGA ACCACAGTAC CAGCTTCAGT CTTTCAAACC TCACACTGCC CACCAAAGGT 
GCCCGAGAGA AGGCCACGCC CTTCCCCAGT CTGAAAGTAT TTGGGCTAAA TACTCTAATG 
GAGATTGTTA CTGAAGCCGG CCCCGGGAGT GGTGAAGGAA ACAGGAGGGC GTTAGTGGAT 
CAGAAGTCAT CTGTCATTAA ACACAGCCCA ACAGTGAAAA GAGAACCTCC ATCACCCCAG 
GGTCGATCCA GCAATTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG 
CTGGACGGCC AGGGAGTTGG CTGGCTCAAC ATGAAAAAGG TGCGCCGGCT GCTGGAGAGC 
GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG CTGAACCGCA TGGTGCAGTC AGAGGACGAT 
GCCCGGCAGG ACATCATCCC GGATGTGGAG ATCAGTCGGA AGGTGTACAA GGGAATGTTA 
GACCTCCTCA AGTGTACAGT CCTCAGCTTG GAGCAGTCCT ATGCCCACGC GGGTCTGGGT 
GGCATGGCCA GCATCTTTGG GCTTTTGGAG ATTGCCCAGA CCCACTACTA TAGTAAAGAA 
CCAGACAAGC GGAAGAGAAG TCCAACAGAA AGTGTAAATA CCCCAGTTGG CAAGGATCCT 
GGCCTAGCTG GGCGGGGGGA CCCAAAGGCT ATGGCACAAC TGAGAGTTCC ACAACTGGGA 
CCTCGGGCAC CAAGTGCCAC AGGAAAGGGT CCTAAGGAAC TGGACACCAG AAGTTTAAAG 
GAAGAAAATT TTATAGCATC TATTGAATTG TGGAACAAGC ACCAGGAAGT GAAAAAGCAA 
AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCTG TCTTTGACCT TGGTGAGACA 
GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC AGTGGTGTGA GCCTGACGTC TAGTTCCCAG 
AGGACTGATC AAGACTCTGT CATCGGCGTG AGTCCAGCTG TTATGATCCG CAGCTCAAGT 
CAGGATTCTG AAGTGAGTAA TAGCTCTGGA GAGACCCTTG GAGCTGACAG TGACTTGAGC 
AGCAATGCAG GTGATGGACC AGGTGGCGAG GGCAGTGTTC ACCTGGCAAG CTCTCGGGGC 
ACTTTGTCTG ATAGTGAAAT TGAGACCAAC TCTGCCACAA GCACCATCTT TGGTAAAGCC 
CACAGCTTGA AGCCAAGCAT AAAGGAGAAG CTGGCAGGCA GCCCCATTCG TACTTCTGAA 
GATGTGAGCC AGCGAGTCTA TCTCTATGAG GGACTCCTAG GAAGGGACAA AGGATCCATG 
TGGGACCAGT TAGAGGATGC AGCTATGGAG ACCTTTTCTA TAAGCAAAGA GCGTTCTACT 
TTATGGGACC AAATGCAATT CTGGGAAGAT GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA 
GAAGGGATGG GTATGGACCA GGGTCCCCAG GAAATGATCG ACAGGTACCT GTCCCTTGGA 
GAACATGACC GGAAGCGCCT GGAAGATGAT GAAGATCGCT TGTTGGCCAC ACTTCTGCAC 
AACCTCATCT CCTACATGCT GCTGATGAAG GTAAATAAGA ATGACATCCG CAAGAAGGTG 
AGGCGCCTAA TGGGAAAGTC GCACATTGGG CTTGTGTACA GCCAGCAAAT CAATGAGGTG 
CTTGATCAGC TGGCGAACCT GAATGGACGC GATCTCTCTA TCTGGTCCAG TGGCAGCCGG 
CACATGAAGA AGCAGACATT TGTGGTACAT GCAGGGACAG ATACAAACGG AGATATCTTT 
TTCATGGAGG TGTGCGATGA CTGTGTGGTG TTGCGTAGTA ACATCGGAAC AGTGTATGAG 
CGCTGGTGGT ACGAGAAGCT CATCAACATG ACCTACTGTC CCAAGACGAA GGTGTTGTGC 
TTGTGGCGTA GAAATGGCTC TGAGACCCAG CTCAACAAGT TCTATACTAA AAAGTGTCGG 
GAGCTGTACT ACTGTGTGAA GGACAGCATG GAGCGCGCTG CCGCCCGACA GCAAAGCATC 
AAACCCGGAC CTGAATTGGG TGGCGAGTTC CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT 
GGCCTGCTGC AGGTGACCCT GGAAGGGATC AACCTCAAAT TCATGCACAA TCAGTTCCTG 
AAATTAAAGA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363561.1
CDS	203..4780
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376632.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 66, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376632.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGCTTGAGAC TGGCAAGTGA CTCAGATGCA GAGTCAGACT CTCGGGCAAG CTCTCCCAAC 
TCCACCGTCT CCAACACCAG CACCGAGGGC TTCGGGGGCA TCATGTCTTT TGCCAGCAGC 
CTCTATCGGA ACCACAGTAC CAGCTTCAGT CTTTCAAACC TCACACTGCC CACCAAAGGT 
GCCCGAGAGA AGGCCACGCC CTTCCCCAGT CTGAAAGTAT TTGGGCTAAA TACTCTAATG 
GAGATTGTTA CTGAAGCCGG CCCCGGGAGT GGTGAAGGAA ACAGGAGGGC GTTAGTGGAT 
CAGAAGTCAT CTGTCATTAA ACACAGCCCA ACAGTGAAAA GAGAACCTCC ATCACCCCAG 
GGTCGATCCA GCAATTCTAG TGAGAACCAG CAGTTCCTGA AGGAGGTGGT GCACAGCGTG 
CTGGACGGCC AGGGAGTTGG CTGGCTCAAC ATGAAAAAGG TGCGCCGGCT GCTGGAGAGC 
GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG CTGAACCGCA TGGTGCAGTC AGAGGACGAT 
GCCCGGCAGG ACATCATCCC GGATGTGGAG ATCAGTCGGA AGGTGTACAA GGGAATGTTA 
GACCTCCTCA AGTGTACAGT CCTCAGCTTG GAGCAGTCCT ATGCCCACGC GGGTCTGGGT 
GGCATGGCCA GCATCTTTGG GCTTTTGGAG ATTGCCCAGA CCCACTACTA TAGTAAAGAA 
CCAGACAAGC GGAAGAGAAG TCCAACAGAA AGTGTAAATA CCCCAGTTGG CAAGGATCCT 
GGCCTAGCTG GGCGGGGGGA CCCAAAGGCT ATGGCACAAC TGAGAGTTCC ACAACTGGGA 
CCTCGGGCAC CAAGTGCCAC AGGAAAGGGT CCTAAGGAAC TGGACACCAG AAGTTTAAAG 
GAAGAAAATT TTATAGCATC TATTGAATTG TGGAACAAGC ACCAGGAAGT GAAAAAGCAA 
AAAGCTTTGG AAAAACAGAG GCCTGAAGTA ATCAAACCTG TCTTTGACCT TGGTGAGACA 
GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC AGTGGTGTGA GCCTGACGTC TAGTTCCCAG 
AGGACTGATC AAGACTCTGT CATCGGCGTG AGTCCAGCTG TTATGATCCG CAGCTCAAGT 
CAGGATTCTG AAGTGAGTAA TAGCTCTGGA GAGACCCTTG GAGCTGACAG TGACTTGAGC 
AGCAATGCAG GTGATGGACC AGGTGGCGAG GGCAGTGTTC ACCTGGCAAG CTCTCGGGGC 
ACTTTGTCTG ATAGTGAAAT TGAGACCAAC TCTGCCACAA GCACCATCTT TGGTAAAGCC 
CACAGCTTGA AGCCAAGCAT AAAGGAGAAG CTGGCAGGCA GCCCCATTCG TACTTCTGAA 
GATGTGAGCC AGCGAGTCTA TCTCTATGAG GGACTCCTAG GAAGGGACAA AGGATCCATG 
TGGGACCAGT TAGAGGATGC AGCTATGGAG ACCTTTTCTA TAAGCAAAGA GCGTTCTACT 
TTATGGGACC AAATGCAATT CTGGGAAGAT GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA 
GAAGGGATGG GTATGGACCA GGGTCCCCAG GAAATGATCG ACAGGTACCT GTCCCTTGGA 
GAACATGACC GGAAGCGCCT GGAAGATGAT GAAGATCGCT TGTTGGCCAC ACTTCTGCAC 
AACCTCATCT CCTACATGCT GCTGATGAAG GTAAATAAGA ATGACATCCG CAAGAAGGTG 
AGGCGCCTAA TGGGAAAGTC GCACATTGGG CTTGTGTACA GCCAGCAAAT CAATGAGGTG 
CTTGATCAGC TGGCGAACCT GAATGGACGC GATCTCTCTA TCTGGTCCAG TGGCAGCCGG 
CACATGAAGA AGCAGACATT TGTGGTACAT GCAGGGACAG ATACAAACGG AGATATCTTT 
TTCATGGAGG TGTGCGATGA CTGTGTGGTG TTGCGTAGTA ACATCGGAAC AGTGTATGAG 
CGCTGGTGGT ACGAGAAGCT CATCAACATG ACCTACTGTC CCAAGACGAA GGTGTTGTGC 
TTGTGGCGTA GAAATGGCTC TGAGACCCAG CTCAACAAGT TCTATACTAA AAAGTGTCGG 
GAGCTGTACT ACTGTGTGAA GGACAGCATG GAGCGCGCTG CCGCCCGACA GCAAAGCATC 
AAACCCGGAC CTGAATTGGG TGGCGAGTTC CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT 
GGCCTGCTGC AGGTGACCCT GGAAGGGATC AACCTCAAAT TCATGCACAA TCAGTTCCTG 
AAATTAAAGA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376650.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363579.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 84, mRNA.

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CloneID	OHu109161
Clone ID Related Accession (Same CDS sequence)	NM_001376650.1
Accession Version	NM_001376650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 63
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGAAACAGG AGGGCGTTAG TGGATCAGAA GTCATCTGTC 
ATTAAACACA GCCCAACAGT GAAAAGAGAA CCTCCATCAC CCCAGGGTCG ATCCAGCAAT 
TCTAGTGAGA ACCAGCAGTT CCTGAAGGAG GTGGTGCACA GCGTGCTGGA CGGCCAGGGA 
GTTGGCTGGC TCAACATGAA AAAGGTGCGC CGGCTGCTGG AGAGCGAGCA GCTGCGAGTC 
TTTGTCCTGA GCAAGCTGAA CCGCATGGTG CAGTCAGAGG ACGATGCCCG GCAGGACATC 
ATCCCGGATG TGGAGATCAG TCGGAAGGTG TACAAGGGAA TGTTAGACCT CCTCAAGTGT 
ACAGTCCTCA GCTTGGAGCA GTCCTATGCC CACGCGGGTC TGGGTGGCAT GGCCAGCATC 
TTTGGGCTTT TGGAGATTGC CCAGACCCAC TACTATAGTA AAGAACCAGA CAAGCGGAAG 
AGAAGTCCAA CAGAAAGTGT AAATACCCCA GTTGGCAAGG ATCCTGGCCT AGCTGGGCGG 
GGGGACCCAA AGGCTATGGC ACAACTGAGA GTTCCACAAC TGGGACCTCG GGCACCAAGT 
GCCACAGGAA AGGGTCCTAA GGAACTGGAC ACCAGAAGTT TAAAGGAAGA AAATTTTATA 
GCATCTATTG GGCCTGAAGT AATCAAACCT GTCTTTGACC TTGGTGAGAC AGAGGAGAAA 
AAGTCCCAGA TCAGCGCAGA CAGTGGTGTG AGCCTGACGT CTAGTTCCCA GAGGACTGAT 
CAAGACTCTG TCATCGGCGT GAGTCCAGCT GTTATGATCC GCAGCTCAAG TCAGGATTCT 
GAAGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363579.1
CDS	203..4726
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFARNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376650.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 84, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376650.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGAAACAGG AGGGCGTTAG TGGATCAGAA GTCATCTGTC 
ATTAAACACA GCCCAACAGT GAAAAGAGAA CCTCCATCAC CCCAGGGTCG ATCCAGCAAT 
TCTAGTGAGA ACCAGCAGTT CCTGAAGGAG GTGGTGCACA GCGTGCTGGA CGGCCAGGGA 
GTTGGCTGGC TCAACATGAA AAAGGTGCGC CGGCTGCTGG AGAGCGAGCA GCTGCGAGTC 
TTTGTCCTGA GCAAGCTGAA CCGCATGGTG CAGTCAGAGG ACGATGCCCG GCAGGACATC 
ATCCCGGATG TGGAGATCAG TCGGAAGGTG TACAAGGGAA TGTTAGACCT CCTCAAGTGT 
ACAGTCCTCA GCTTGGAGCA GTCCTATGCC CACGCGGGTC TGGGTGGCAT GGCCAGCATC 
TTTGGGCTTT TGGAGATTGC CCAGACCCAC TACTATAGTA AAGAACCAGA CAAGCGGAAG 
AGAAGTCCAA CAGAAAGTGT AAATACCCCA GTTGGCAAGG ATCCTGGCCT AGCTGGGCGG 
GGGGACCCAA AGGCTATGGC ACAACTGAGA GTTCCACAAC TGGGACCTCG GGCACCAAGT 
GCCACAGGAA AGGGTCCTAA GGAACTGGAC ACCAGAAGTT TAAAGGAAGA AAATTTTATA 
GCATCTATTG GGCCTGAAGT AATCAAACCT GTCTTTGACC TTGGTGAGAC AGAGGAGAAA 
AAGTCCCAGA TCAGCGCAGA CAGTGGTGTG AGCCTGACGT CTAGTTCCCA GAGGACTGAT 
CAAGACTCTG TCATCGGCGT GAGTCCAGCT GTTATGATCC GCAGCTCAAG TCAGGATTCT 
GAAGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376620.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363549.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 54, mRNA.

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CloneID	OHu109163
Clone ID Related Accession (Same CDS sequence)	NM_001376620.1
Accession Version	NM_001376620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 40
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGT CAGTGCGCCG GCGAATCTAT 
GACAATCCAT ACTTCGAGCC CCAATATGGC TTTCCCCCTG AGGAAGATGA GGATGAGCAG 
GGGGAAAGTT ACACTCCCCG ATTCAGCCAA CATGTCAGTG GCAATCGGGC TCAAAAGCTG 
CTGCGGCCCA ACAGCTTGAG ACTGGCAAGT GACTCAGATG CAGAGTCAGA CTCTCGGGCA 
AGCTCTCCCA ACTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT 
TTTGCCAGCA GCCTCTATCG GAACCACAGT ACCAGCTTCA GTCTTTCAAA CCTCACACTG 
CCCACCAAAG GTGCCCGAGA GAAGGCCACG CCCTTCCCCA GTCTGAAAGG AAACAGGAGG 
GCGTTAGTGG ATCAGAAGTC ATCTGTCATT AAACACAGCC CAACAGTGAA AAGAGAACCT 
CCATCACCCC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAGGTG 
GTGCACAGCG TGCTGGACGG CCAGGGAGTT GGCTGGCTCA ACATGAAAAA GGTGCGCCGG 
CTGCTGGAGA GCGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG CATGGTGCAG 
TCAGAGGACG ATGCCCGGCA GGACATCATC CCGGATGTGG AGATCAGTCG GAAGGTGTAC 
AAGGGAATGT TAGACCTCCT CAAGTGTACA GTCCTCAGCT TGGAGCAGTC CTATGCCCAC 
GCGGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTTTGG AGATTGCCCA GACCCACTAC 
TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AAAGTGTAAA TACCCCAGTT 
GGCAAGGATC CTGGCCTAGC TGGGCGGGGG GACCCAAAGG CTATGGCACA ACTGAGAGTT 
CCACAACTGG GACCTCGGGC ACCAAGTGCC ACAGGAAAGG GTCCTAAGGA ACTGGACACC 
AGAAGTTTAA AGGAAGAAAA TTTTATAGCA TCTATTGGGC CTGAAGTAAT CAAACCTGTC 
TTTGACCTTG GTGAGACAGA GGAGAAAAAG TCCCAGATCA GCGCAGACAG TGGTGTGAGC 
CTGACGTCTA GTTCCCAGAG GACTGATCAA GACTCTGTCA TCGGCGTGAG TCCAGCTGTT 
ATGATCCGCA GCTCAAGTCA GGATTCTGAA GTTAGCACCG TGGTGAGTAA TAGCTCTGGA 
GAGACCCTTG GAGCTGACAG TGACTTGAGC AGCAATGCAG GTGATGGACC AGGTGGCGAG 
GGCAGTGTTC ACCTGGCAAG CTCTCGGGGC ACTTTGTCTG ATAGTGAAAT TGAGACCAAC 
TCTGCCACAA GCACCATCTT TGGTAAAGCC CACAGCTTGA AGCCAAGCAT AAAGGAGAAG 
CTGGCAGGCA GCCCCATTCG TACTTCTGAA GATGTGAGCC AGCGAGTCTA TCTCTATGAG 
GGACTCCTAG GAAGGGACAA AGGATCCATG TGGGACCAGT TAGAGGATGC AGCTATGGAG 
ACCTTTTCTA TAAGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363549.1
CDS	231..4856
Translation	MSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376620.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 54, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376620.1

ORF Insert Sequence:

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGT CAGTGCGCCG GCGAATCTAT 
GACAATCCAT ACTTCGAGCC CCAATATGGC TTTCCCCCTG AGGAAGATGA GGATGAGCAG 
GGGGAAAGTT ACACTCCCCG ATTCAGCCAA CATGTCAGTG GCAATCGGGC TCAAAAGCTG 
CTGCGGCCCA ACAGCTTGAG ACTGGCAAGT GACTCAGATG CAGAGTCAGA CTCTCGGGCA 
AGCTCTCCCA ACTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT 
TTTGCCAGCA GCCTCTATCG GAACCACAGT ACCAGCTTCA GTCTTTCAAA CCTCACACTG 
CCCACCAAAG GTGCCCGAGA GAAGGCCACG CCCTTCCCCA GTCTGAAAGG AAACAGGAGG 
GCGTTAGTGG ATCAGAAGTC ATCTGTCATT AAACACAGCC CAACAGTGAA AAGAGAACCT 
CCATCACCCC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAGGTG 
GTGCACAGCG TGCTGGACGG CCAGGGAGTT GGCTGGCTCA ACATGAAAAA GGTGCGCCGG 
CTGCTGGAGA GCGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG CATGGTGCAG 
TCAGAGGACG ATGCCCGGCA GGACATCATC CCGGATGTGG AGATCAGTCG GAAGGTGTAC 
AAGGGAATGT TAGACCTCCT CAAGTGTACA GTCCTCAGCT TGGAGCAGTC CTATGCCCAC 
GCGGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTTTGG AGATTGCCCA GACCCACTAC 
TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AAAGTGTAAA TACCCCAGTT 
GGCAAGGATC CTGGCCTAGC TGGGCGGGGG GACCCAAAGG CTATGGCACA ACTGAGAGTT 
CCACAACTGG GACCTCGGGC ACCAAGTGCC ACAGGAAAGG GTCCTAAGGA ACTGGACACC 
AGAAGTTTAA AGGAAGAAAA TTTTATAGCA TCTATTGGGC CTGAAGTAAT CAAACCTGTC 
TTTGACCTTG GTGAGACAGA GGAGAAAAAG TCCCAGATCA GCGCAGACAG TGGTGTGAGC 
CTGACGTCTA GTTCCCAGAG GACTGATCAA GACTCTGTCA TCGGCGTGAG TCCAGCTGTT 
ATGATCCGCA GCTCAAGTCA GGATTCTGAA GTTAGCACCG TGGTGAGTAA TAGCTCTGGA 
GAGACCCTTG GAGCTGACAG TGACTTGAGC AGCAATGCAG GTGATGGACC AGGTGGCGAG 
GGCAGTGTTC ACCTGGCAAG CTCTCGGGGC ACTTTGTCTG ATAGTGAAAT TGAGACCAAC 
TCTGCCACAA GCACCATCTT TGGTAAAGCC CACAGCTTGA AGCCAAGCAT AAAGGAGAAG 
CTGGCAGGCA GCCCCATTCG TACTTCTGAA GATGTGAGCC AGCGAGTCTA TCTCTATGAG 
GGACTCCTAG GAAGGGACAA AGGATCCATG TGGGACCAGT TAGAGGATGC AGCTATGGAG 
ACCTTTTCTA TAAGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376577.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363506.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 17, mRNA.

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CloneID	OHu109164
Clone ID Related Accession (Same CDS sequence)	NM_001376577.1
Accession Version	NM_001376577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.232559.1, SRR1660805.131914.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ACGAAATCTG CTACTCCGTA 
TTATGTCTCT TCTCGTACGT GGCTGCAGTT CATAGCAGTG AGGAAGATCT CAGAACCCCG 
CCCCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363506.1
CDS	203..5083
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376577.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376577.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ACGAAATCTG CTACTCCGTA 
TTATGTCTCT TCTCGTACGT GGCTGCAGTT CATAGCAGTG AGGAAGATCT CAGAACCCCG 
CCCCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376607.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363536.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 41, mRNA.

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CloneID	OHu109169
Clone ID Related Accession (Same CDS sequence)	NM_001376607.1
Accession Version	NM_001376607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 32
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363536.1
CDS	203..4888
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376607.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376607.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376646.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363575.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 80, mRNA.

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CloneID	OHu109172
Clone ID Related Accession (Same CDS sequence)	NM_001376646.1
Accession Version	NM_001376646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 59
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.65356.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGGG CCTGAAGTAA TCAAACCTGT CTTTGACCTT 
GGTGAGACAG AGGAGAAAAA GTCCCAGATC AGCGCAGACA GTGGTGTGAG CCTGACGTCT 
AGTTCCCAGA GGACTGATCA AGACTCTGTC ATCGGCGTGA GTCCAGCTGT TATGATCCGC 
AGCTCAAGTC AGGATTCTGA AGTGAGTAAT AGCTCTGGAG AGACCCTTGG AGCTGACAGT 
GACTTGAGCA GCAATGCAGG TGATGGACCA GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC 
TCTCGGGGCA CTTTGTCTGA TAGTGAAATT GAGACCAACT CTGCCACAAG CACCATCTTT 
GGTAAAGCCC ACAGCTTGAA GCCAAGCATA AAGGAGAAGC TGGCAGGCAG CCCCATTCGT 
ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT CTCTATGAGG GACTCCTAGG AAGGGACAAA 
GGATCCATGT GGGACCAGTT AGAGGATGCA GCTATGGAGA CCTTTTCTAT AAGCAAAGAG 
CGTTCTACTT TATGGGACCA AATGCAATTC TGGGAAGATG CCTTCTTAGA TGCTGTGATG 
TTGGAGAGAG AAGGGATGGG TATGGACCAG GGTCCCCAGG AAATGATCGA CAGGTACCTG 
TCCCTTGGAG AACATGACCG GAAGCGCCTG GAAGATGATG AAGATCGCTT GTTGGCCACA 
CTTCTGCACA ACCTCATCTC CTACATGCTG CTGATGAAGG TAAATAAGAA TGACATCCGC 
AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG CACATTGGGC TTGTGTACAG CCAGCAAATC 
AATGAGGTGC TTGATCAGCT GGCGAACCTG AATGGACGCG ATCTCTCTAT CTGGTCCAGT 
GGCAGCCGGC ACATGAAGAA GCAGACATTT GTGGTACATG CAGGGACAGA TACAAACGGA 
GATATCTTTT TCATGGAGGT GTGCGATGAC TGTGTGGTGT TGCGTAGTAA CATCGGAACA 
GTGTATGAGC GCTGGTGGTA CGAGAAGCTC ATCAACATGA CCTACTGTCC CAAGACGAAG 
GTGTTGTGCT TGTGGCGTAG AAATGGCTCT GAGACCCAGC TCAACAAGTT CTATACTAAA 
AAGTGTCGGG AGCTGTACTA CTGTGTGAAG GACAGCATGG AGCGCGCTGC CGCCCGACAG 
CAAAGCATCA AACCCGGACC TGAATTGGGT GGCGAGTTCC CTGTGCAGGA CCTGAAGACT 
GGTGAGGGTG GCCTGCTGCA GGTGACCCTG GAAGGGATCA ACCTCAAATT CATGCACAAT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTTCGAGG CGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AATTAAAGAA GTGGTGAGCC ACAAGTACAA GACACCAATG 
GCCCACGAAA TCTGCTACTC CGTATTATGT CTCTTCTCGT ACGTGGCTGC AGTTCATAGC 
AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363575.1
CDS	257..4801
Translation	MSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376646.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 80, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376646.1

ORF Insert Sequence:

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGGG CCTGAAGTAA TCAAACCTGT CTTTGACCTT 
GGTGAGACAG AGGAGAAAAA GTCCCAGATC AGCGCAGACA GTGGTGTGAG CCTGACGTCT 
AGTTCCCAGA GGACTGATCA AGACTCTGTC ATCGGCGTGA GTCCAGCTGT TATGATCCGC 
AGCTCAAGTC AGGATTCTGA AGTGAGTAAT AGCTCTGGAG AGACCCTTGG AGCTGACAGT 
GACTTGAGCA GCAATGCAGG TGATGGACCA GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC 
TCTCGGGGCA CTTTGTCTGA TAGTGAAATT GAGACCAACT CTGCCACAAG CACCATCTTT 
GGTAAAGCCC ACAGCTTGAA GCCAAGCATA AAGGAGAAGC TGGCAGGCAG CCCCATTCGT 
ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT CTCTATGAGG GACTCCTAGG AAGGGACAAA 
GGATCCATGT GGGACCAGTT AGAGGATGCA GCTATGGAGA CCTTTTCTAT AAGCAAAGAG 
CGTTCTACTT TATGGGACCA AATGCAATTC TGGGAAGATG CCTTCTTAGA TGCTGTGATG 
TTGGAGAGAG AAGGGATGGG TATGGACCAG GGTCCCCAGG AAATGATCGA CAGGTACCTG 
TCCCTTGGAG AACATGACCG GAAGCGCCTG GAAGATGATG AAGATCGCTT GTTGGCCACA 
CTTCTGCACA ACCTCATCTC CTACATGCTG CTGATGAAGG TAAATAAGAA TGACATCCGC 
AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG CACATTGGGC TTGTGTACAG CCAGCAAATC 
AATGAGGTGC TTGATCAGCT GGCGAACCTG AATGGACGCG ATCTCTCTAT CTGGTCCAGT 
GGCAGCCGGC ACATGAAGAA GCAGACATTT GTGGTACATG CAGGGACAGA TACAAACGGA 
GATATCTTTT TCATGGAGGT GTGCGATGAC TGTGTGGTGT TGCGTAGTAA CATCGGAACA 
GTGTATGAGC GCTGGTGGTA CGAGAAGCTC ATCAACATGA CCTACTGTCC CAAGACGAAG 
GTGTTGTGCT TGTGGCGTAG AAATGGCTCT GAGACCCAGC TCAACAAGTT CTATACTAAA 
AAGTGTCGGG AGCTGTACTA CTGTGTGAAG GACAGCATGG AGCGCGCTGC CGCCCGACAG 
CAAAGCATCA AACCCGGACC TGAATTGGGT GGCGAGTTCC CTGTGCAGGA CCTGAAGACT 
GGTGAGGGTG GCCTGCTGCA GGTGACCCTG GAAGGGATCA ACCTCAAATT CATGCACAAT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTTCGAGG CGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AATTAAAGAA GTGGTGAGCC ACAAGTACAA GACACCAATG 
GCCCACGAAA TCTGCTACTC CGTATTATGT CTCTTCTCGT ACGTGGCTGC AGTTCATAGC 
AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376586.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363515.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 26, mRNA.

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CloneID	OHu109173
Clone ID Related Accession (Same CDS sequence)	NM_001376586.1
Accession Version	NM_001376586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 23
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.37321.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363515.1
CDS	203..4960
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376586.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376586.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGGAG CGGAAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376579.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363508.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 19, mRNA.

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CloneID	OHu109175
Clone ID Related Accession (Same CDS sequence)	NM_001376579.1
Accession Version	NM_001376579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.44979.1, SRR1660805.62961.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363508.1
CDS	203..5029
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376579.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376579.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376602.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363531.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 36, mRNA.

Price & Availability↑

CloneID	OHu109176
Clone ID Related Accession (Same CDS sequence)	NM_001376602.1
Accession Version	NM_001376602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.118519.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCACACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGGG CCTGAAGTAA TCAAACCTGT CTTTGACCTT 
GGTGAGACAG AGGAGAAAAA GTCCCAGATC AGCGCAGACA GTGGTGTGAG CCTGACGTCT 
AGTTCCCAGA GGACTGATCA AGACTCTGTC ATCGGCGTGA GTCCAGCTGT TATGATCCGC 
AGCTCAAGTC AGGATTCTGA AGTGAGTAAT AGCTCTGGAG AGACCCTTGG AGCTGACAGT 
GACTTGAGCA GCAATGCAGG TGATGGACCA GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC 
TCTCGGGGCA CTTTGTCTGA TAGTGAAATT GAGACCAACT CTGCCACAAG CACCATCTTT 
GGTAAAGCCC ACAGCTTGAA GCCAAGCATA AAGGAGAAGC TGGCAGGCAG CCCCATTCGT 
ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT CTCTATGAGG GACTCCTAGG AAGGGACAAA 
GGATCCATGT GGGACCAGTT AGAGGATGCA GCTATGGAGA CCTTTTCTAT AAGCAAAGAG 
CGTTCTACTT TATGGGACCA AATGCAATTC TGGGAAGATG CCTTCTTAGA TGCTGTGATG 
TTGGAGAGAG AAGGGATGGG TATGGACCAG GGTCCCCAGG AAATGATCGA CAGGTACCTG 
TCCCTTGGAG AACATGACCG GAAGCGCCTG GAAGATGATG AAGATCGCTT GTTGGCCACA 
CTTCTGCACA ACCTCATCTC CTACATGCTG CTGATGAAGG TAAATAAGAA TGACATCCGC 
AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG CACATTGGGC TTGTGTACAG CCAGCAAATC 
AATGAGGTGC TTGATCAGCT GGCGAACCTG AATGGACGCG ATCTCTCTAT CTGGTCCAGT 
GGCAGCCGGC ACATGAAGAA GCAGACATTT GTGGTACATG CAGGGACAGA TACAAACGGA 
GATATCTTTT TCATGGAGGT GTGCGATGAC TGTGTGGTGT TGCGTAGTAA CATCGGAACA 
GTGTATGAGC GCTGGTGGTA CGAGAAGCTC ATCAACATGA CCTACTGTCC CAAGACGAAG 
GTGTTGTGCT TGTGGCGTAG AAATGGCTCT GAGACCCAGC TCAACAAGTT CTATACTAAA 
AAGTGTCGGG AGCTGTACTA CTGTGTGAAG GACAGCATGG AGCGCGCTGC CGCCCGACAG 
CAAAGCATCA AACCCGGACC TGAATTGGGT GGCGAGTTCC CTGTGCAGGA CCTGAAGACT 
GGTGAGGGTG GCCTGCTGCA GGTGACCCTG GAAGGGATCA ACCTCAAATT CATGCACAAT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTTCGAGG CGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AATTAAAGAA GTGGTGAGCC ACAAGTACAA GACACCAATG 
GCCCACGAAA TCTGCTACTC CGTATTATGT CTCTTCTCGT ACGTGGCTGC AGTTCATAGC 
AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363531.1
CDS	203..4927
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPTLGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376602.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376602.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCACACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGGG CCTGAAGTAA TCAAACCTGT CTTTGACCTT 
GGTGAGACAG AGGAGAAAAA GTCCCAGATC AGCGCAGACA GTGGTGTGAG CCTGACGTCT 
AGTTCCCAGA GGACTGATCA AGACTCTGTC ATCGGCGTGA GTCCAGCTGT TATGATCCGC 
AGCTCAAGTC AGGATTCTGA AGTGAGTAAT AGCTCTGGAG AGACCCTTGG AGCTGACAGT 
GACTTGAGCA GCAATGCAGG TGATGGACCA GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC 
TCTCGGGGCA CTTTGTCTGA TAGTGAAATT GAGACCAACT CTGCCACAAG CACCATCTTT 
GGTAAAGCCC ACAGCTTGAA GCCAAGCATA AAGGAGAAGC TGGCAGGCAG CCCCATTCGT 
ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT CTCTATGAGG GACTCCTAGG AAGGGACAAA 
GGATCCATGT GGGACCAGTT AGAGGATGCA GCTATGGAGA CCTTTTCTAT AAGCAAAGAG 
CGTTCTACTT TATGGGACCA AATGCAATTC TGGGAAGATG CCTTCTTAGA TGCTGTGATG 
TTGGAGAGAG AAGGGATGGG TATGGACCAG GGTCCCCAGG AAATGATCGA CAGGTACCTG 
TCCCTTGGAG AACATGACCG GAAGCGCCTG GAAGATGATG AAGATCGCTT GTTGGCCACA 
CTTCTGCACA ACCTCATCTC CTACATGCTG CTGATGAAGG TAAATAAGAA TGACATCCGC 
AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG CACATTGGGC TTGTGTACAG CCAGCAAATC 
AATGAGGTGC TTGATCAGCT GGCGAACCTG AATGGACGCG ATCTCTCTAT CTGGTCCAGT 
GGCAGCCGGC ACATGAAGAA GCAGACATTT GTGGTACATG CAGGGACAGA TACAAACGGA 
GATATCTTTT TCATGGAGGT GTGCGATGAC TGTGTGGTGT TGCGTAGTAA CATCGGAACA 
GTGTATGAGC GCTGGTGGTA CGAGAAGCTC ATCAACATGA CCTACTGTCC CAAGACGAAG 
GTGTTGTGCT TGTGGCGTAG AAATGGCTCT GAGACCCAGC TCAACAAGTT CTATACTAAA 
AAGTGTCGGG AGCTGTACTA CTGTGTGAAG GACAGCATGG AGCGCGCTGC CGCCCGACAG 
CAAAGCATCA AACCCGGACC TGAATTGGGT GGCGAGTTCC CTGTGCAGGA CCTGAAGACT 
GGTGAGGGTG GCCTGCTGCA GGTGACCCTG GAAGGGATCA ACCTCAAATT CATGCACAAT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTTCGAGG CGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AATTAAAGAA GTGGTGAGCC ACAAGTACAA GACACCAATG 
GCCCACGAAA TCTGCTACTC CGTATTATGT CTCTTCTCGT ACGTGGCTGC AGTTCATAGC 
AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376627.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363556.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 62, mRNA.

Price & Availability↑

CloneID	OHu109183
Clone ID Related Accession (Same CDS sequence)	NM_001376627.1
Accession Version	NM_001376627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 45
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.86995.1, SRR1660803.152287.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTAATCAAAC CTGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC GTCTAGTTCC 
CAGAGGACTG ATCAAGACTC TGTCATCGGC GTGAGTCCAG CTGTTATGAT CCGCAGCTCA 
AGTCAGGATT CTGAAGTGAG TAATAGCTCT GGAGAGACCC TTGGAGCTGA CAGTGACTTG 
AGCAGCAATG CAGGTGATGG ACCAGGTGGC GAGGGCAGTG TTCACCTGGC AAGCTCTCGG 
GGCACTTTGT CTGATAGTGA AATTGAGACC AACTCTGCCA CAAGCACCAT CTTTGGTAAA 
GCCCACAGCT TGAAGCCAAG CATAAAGGAG AAGCTGGCAG GCAGCCCCAT TCGTACTTCT 
GAAGATGTGA GCCAGCGAGT CTATCTCTAT GAGGGACTCC TAGGAAGGGA CAAAGGATCC 
ATGTGGGACC AGTTAGAGGA TGCAGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT 
ACTTTATGGG ACCAAATGCA ATTCTGGGAA GATGCCTTCT TAGATGCTGT GATGTTGGAG 
AGAGAAGGGA TGGGTATGGA CCAGGGTCCC CAGGAAATGA TCGACAGGTA CCTGTCCCTT 
GGAGAACATG ACCGGAAGCG CCTGGAAGAT GATGAAGATC GCTTGTTGGC CACACTTCTG 
CACAACCTCA TCTCCTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGCAAGAAG 
GTGAGGCGCC TAATGGGAAA GTCGCACATT GGGCTTGTGT ACAGCCAGCA AATCAATGAG 
GTGCTTGATC AGCTGGCGAA CCTGAATGGA CGCGATCTCT CTATCTGGTC CAGTGGCAGC 
CGGCACATGA AGAAGCAGAC ATTTGTGGTA CATGCAGGGA CAGATACAAA CGGAGATATC 
TTTTTCATGG AGGTGTGCGA TGACTGTGTG GTGTTGCGTA GTAACATCGG AACAGTGTAT 
GAGCGCTGGT GGTACGAGAA GCTCATCAAC ATGACCTACT GTCCCAAGAC GAAGGTGTTG 
TGCTTGTGGC GTAGAAATGG CTCTGAGACC CAGCTCAACA AGTTCTATAC TAAAAAGTGT 
CGGGAGCTGT ACTACTGTGT GAAGGACAGC ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC 
ATCAAACCCG GACCTGAATT GGGTGGCGAG TTCCCTGTGC AGGACCTGAA GACTGGTGAG 
GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG ATCAACCTCA AATTCATGCA CAATCAGGAG 
CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTTCG AGGCGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCA 
ATGGCCCACG AAATCTGCTA CTCCGTATTA TGTCTCTTCT CGTACGTGGC TGCAGTTCAT 
AGCAGTGAGG AAGATCTCAG AACCCCGCCC CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363556.1
CDS	257..4864
Translation	MSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376627.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 62, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376627.1

ORF Insert Sequence:

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTAATCAAAC CTGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC GTCTAGTTCC 
CAGAGGACTG ATCAAGACTC TGTCATCGGC GTGAGTCCAG CTGTTATGAT CCGCAGCTCA 
AGTCAGGATT CTGAAGTGAG TAATAGCTCT GGAGAGACCC TTGGAGCTGA CAGTGACTTG 
AGCAGCAATG CAGGTGATGG ACCAGGTGGC GAGGGCAGTG TTCACCTGGC AAGCTCTCGG 
GGCACTTTGT CTGATAGTGA AATTGAGACC AACTCTGCCA CAAGCACCAT CTTTGGTAAA 
GCCCACAGCT TGAAGCCAAG CATAAAGGAG AAGCTGGCAG GCAGCCCCAT TCGTACTTCT 
GAAGATGTGA GCCAGCGAGT CTATCTCTAT GAGGGACTCC TAGGAAGGGA CAAAGGATCC 
ATGTGGGACC AGTTAGAGGA TGCAGCTATG GAGACCTTTT CTATAAGCAA AGAGCGTTCT 
ACTTTATGGG ACCAAATGCA ATTCTGGGAA GATGCCTTCT TAGATGCTGT GATGTTGGAG 
AGAGAAGGGA TGGGTATGGA CCAGGGTCCC CAGGAAATGA TCGACAGGTA CCTGTCCCTT 
GGAGAACATG ACCGGAAGCG CCTGGAAGAT GATGAAGATC GCTTGTTGGC CACACTTCTG 
CACAACCTCA TCTCCTACAT GCTGCTGATG AAGGTAAATA AGAATGACAT CCGCAAGAAG 
GTGAGGCGCC TAATGGGAAA GTCGCACATT GGGCTTGTGT ACAGCCAGCA AATCAATGAG 
GTGCTTGATC AGCTGGCGAA CCTGAATGGA CGCGATCTCT CTATCTGGTC CAGTGGCAGC 
CGGCACATGA AGAAGCAGAC ATTTGTGGTA CATGCAGGGA CAGATACAAA CGGAGATATC 
TTTTTCATGG AGGTGTGCGA TGACTGTGTG GTGTTGCGTA GTAACATCGG AACAGTGTAT 
GAGCGCTGGT GGTACGAGAA GCTCATCAAC ATGACCTACT GTCCCAAGAC GAAGGTGTTG 
TGCTTGTGGC GTAGAAATGG CTCTGAGACC CAGCTCAACA AGTTCTATAC TAAAAAGTGT 
CGGGAGCTGT ACTACTGTGT GAAGGACAGC ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC 
ATCAAACCCG GACCTGAATT GGGTGGCGAG TTCCCTGTGC AGGACCTGAA GACTGGTGAG 
GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG ATCAACCTCA AATTCATGCA CAATCAGGAG 
CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTTCG AGGCGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCA 
ATGGCCCACG AAATCTGCTA CTCCGTATTA TGTCTCTTCT CGTACGTGGC TGCAGTTCAT 
AGCAGTGAGG AAGATCTCAG AACCCCGCCC CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376644.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363573.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 78, mRNA.

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CloneID	OHu109185
Clone ID Related Accession (Same CDS sequence)	NM_001376644.1
Accession Version	NM_001376644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 57
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.12857.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTAATCAAAC CTGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC GTCTAGTTCC 
CAGAGGACTG ATCAAGACTC TGTCATCGGC GTGAGTCCAG CTGTTATGAT CCGCAGCTCA 
AGTCAGGATT CTGAAGTTAG CACCGTGAGT AATAGCTCTG GAGAGACCCT TGGAGCTGAC 
AGTGACTTGA GCAGCAATGC AGGTGATGGA CCAGGTGGCG AGGGCAGTGT TCACCTGGCA 
AGCTCTCGGG GCACTTTGTC TGATAGTGAA ATTGAGACCA ACTCTGCCAC AAGCACCATC 
TTTGGTAAAG CCCACAGCTT GAAGCCAAGC ATAAAGGAGA AGCTGGCAGG CAGCCCCATT 
CGTACTTCTG AAGATGTGAG CCAGCGAGTC TATCTCTATG AGGGACTCCT AGGAAGGGAC 
AAAGGATCCA TGTGGGACCA GTTAGAGGAT GCAGCTATGG AGACCTTTTC TATAAGCAAA 
GAGCGTTCTA CTTTATGGGA CCAAATGCAA TTCTGGGAAG ATGCCTTCTT AGATGCTGTG 
ATGTTGGAGA GAGAAGGGAT GGGTATGGAC CAGGGTCCCC AGGAAATGAT CGACAGGTAC 
CTGTCCCTTG GAGAACATGA CCGGAAGCGC CTGGAAGATG ATGAAGATCG CTTGTTGGCC 
ACACTTCTGC ACAACCTCAT CTCCTACATG CTGCTGATGA AGGTAAATAA GAATGACATC 
CGCAAGAAGG TGAGGCGCCT AATGGGAAAG TCGCACATTG GGCTTGTGTA CAGCCAGCAA 
ATCAATGAGG TGCTTGATCA GCTGGCGAAC CTGAATGGAC GCGATCTCTC TATCTGGTCC 
AGTGGCAGCC GGCACATGAA GAAGCAGACA TTTGTGGTAC ATGCAGGGAC AGATACAAAC 
GGAGATATCT TTTTCATGGA GGTGTGCGAT GACTGTGTGG TGTTGCGTAG TAACATCGGA 
ACAGTGTATG AGCGCTGGTG GTACGAGAAG CTCATCAACA TGACCTACTG TCCCAAGACG 
AAGGTGTTGT GCTTGTGGCG TAGAAATGGC TCTGAGACCC AGCTCAACAA GTTCTATACT 
AAAAAGTGTC GGGAGCTGTA CTACTGTGTG AAGGACAGCA TGGAGCGCGC TGCCGCCCGA 
CAGCAAAGCA TCAAACCCGG ACCTGAATTG GGTGGCGAGT TCCCTGTGCA GGACCTGAAG 
ACTGGTGAGG GTGGCCTGCT GCAGGTGACC CTGGAAGGGA TCAACCTCAA ATTCATGCAC 
AATCAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTTCG AGGCGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCA 
ATGGCCCACG AAATCTGCTA CTCCGTATTA TGTCTCTTCT CGTACGTGGC TGCAGTTCAT 
AGCAGTGAGG AAGATCTCAG AACCCCGCCC CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363573.1
CDS	257..4804
Translation	MSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376644.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 78, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376644.1

ORF Insert Sequence:

ATGAGCCTTC GGGATGATAC CTCTTTTGTC TTCACCCTCA CTGACAAGGA CACTGGAGTC 
ACGCGATATG GCATCTGTGT TAACTTCTAC CGCTCCTTCC AAAAGCGAAT CTCTAAGGAG 
AAGGGGGAAG GTGGGGCAGG GTCCCGTGGG AAGGAAGGAA CCCATGCCAC CTGTGCCTCA 
GAAGAGGGTG GCACTGAGAG CTCAGAGAGT GGCTCATCCC TGCAGCCTCT CAGTGCTGAC 
TCTACCCCTG ATGTGAACCA GTCTCCTCGG GGCAAACGCC GGGCCAAGGC GGGGAGCCGC 
TCCCGCAACA GTACTCTCAC GTCCCTGTGC GTGCTCAGCC ACTACCCTTT CTTCTCCACC 
TTCCGAGAGT GTTTGTATAC TCTCAAGCGC CTGGTGGACT GCTGTAGTGA GCGCCTTCTG 
GGCAAGAAAC TGGGCATCCC TCGAGGCGTA CAAAGGGACA CCATGTGGCG GATCTTTACT 
GGATCGCTGC TGGTAGAGGA GAAGTCAAGT GCCCTTCTGC ATGACCTTCG AGAGATTGAG 
GCCTGGATCT ATCGATTGCT GCGCTCCCCA GTACCCGTCT CTGGGCAGAA GCGAGTAGAC 
ATCGAGGTCC TACCCCAAGA GCTCCAGCCA GCTCTGACCT TTGCTCTTCC AGACCCATCT 
CGATTCACCC TAGTGGATTT CCCACTGCAC CTTCCCTTGG AACTTCTAGG TGTGGACGCC 
TGTCTCCAGG TGCTAACCTG CATTCTGTTA GAGCACAAGG TGGTGCTACA GTCCCGAGAC 
TACAATGCAC TCTCCATGTC TGTGATGGCA TTCGTGGCAA TGATCTACCC ACTGGAGTAT 
ATGTTTCCTG TCATCCCGCT GCTACCCACC TGCATGGCAT CAGCAGAGCA GCTGCTGTTG 
GCTCCAACCC CGTACATCAT TGGGGTTCCT GCCAGCTTCT TCCTCTACAA ACTGGACTTC 
AAAATGCCTG ATGATGTATG GCTAGTGGAT CTGGACAGCA ATAGGGTGAT TGCCCCCACC 
AATGCAGAAG TGCTGCCTAT CCTGCCAGAA CCAGAATCAC TAGAGCTGAA AAAGCATTTA 
AAGCAGGCCT TGGCCAGCAT GAGTCTCAAC ACCCAGCCCA TCCTCAATCT GGAGAAATTT 
CATGAGGGCC AGGAGATCCC CCTTCTCTTG GGAAGGCCTT CTAATGACCT GCAGTCCACA 
CCGTCCACTG AATTCAACCC ACTCATCTAT GGCAATGATG TGGATTCTGT GGATGTTGCA 
ACCAGGGTTG CCATGGTACG GTTCTTCAAT TCCGCCAACG TGCTGCAGGG ATTTCAGATG 
CACACGCGTA CCCTGCGCCT CTTTCCTCGG CCTGTGGTAG CTTTTCAAGC TGGCTCCTTT 
CTAGCCTCAC GTCCCCGGCA GACTCCTTTT GCCGAGAAAT TGGCCAGGAC TCAGGCTGTG 
GAGTACTTTG GGGAATGGAT CCTTAACCCC ACCAACTATG CCTTTCAGCG AATTCACAAC 
AATATGTTTG ATCCAGCCCT GATTGGTGAC AAGCCAAAGT GGTATGCTCA TCAGCTGCAG 
CCTATCCACT ATCGCGTCTA TGACAGCAAT TCCCAGCTGG CTGAGGCCCT GAGTGTACCA 
CCAGAGCGGG ACTCTGACTC CGAACCTACT GATGATAGTG GCAGTGATAG TATGGATTAT 
GACGATTCAA GCTCTTCTTA CTCCTCCCTT GGTGACTTTG TCAGTGAAAT GATGAAATGT 
GACATTAATG GTGATACTCC CAATGTGGAC CCTCTGACAC ATGCAGCACT GGGGGATGCC 
AGCGAGGTGG AGATTGACGA GCTGCAGAAT CAGAAGGAAG CAGAAGAGCC TGGCCCAGAC 
AGTGAGAACT CTCAGGAAAA CCCCCCACTG CGCTCCAGCT CTAGCACCAC AGCCAGCAGC 
AGCCCCAGCA CTGTCATCCA CGGAGCCAAC TCTGAACCTG CTGACTCTAC GGAGATGGAT 
GATAAGGCAG CAGTAGGCGT CTCCAAGCCC CTCCCTTCCG TGCCTCCCAG CATTGGCAAA 
TCGAACGTGG ACAGACGTCA GGCAGAAATT GGAGAGGGGG CTCAAAAGCT GCTGCGGCCC 
AACAGCTTGA GACTGGCAAG TGACTCAGAT GCAGAGTCAG ACTCTCGGGC AAGCTCTCCC 
AACTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTCTATC GGAACCACAG TACCAGCTTC AGTCTTTCAA ACCTCACACT GCCCACCAAA 
GGTGCCCGAG AGAAGGCCAC GCCCTTCCCC AGTCTGAAAG GAAACAGGAG GGCGTTAGTG 
GATCAGAAGT CATCTGTCAT TAAACACAGC CCAACAGTGA AAAGAGAACC TCCATCACCC 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAGGT GGTGCACAGC 
GTGCTGGACG GCCAGGGAGT TGGCTGGCTC AACATGAAAA AGGTGCGCCG GCTGCTGGAG 
AGCGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GCATGGTGCA GTCAGAGGAC 
GATGCCCGGC AGGACATCAT CCCGGATGTG GAGATCAGTC GGAAGGTGTA CAAGGGAATG 
TTAGACCTCC TCAAGTGTAC AGTCCTCAGC TTGGAGCAGT CCTATGCCCA CGCGGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTTTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAAAGTGTAA ATACCCCAGT TGGCAAGGAT 
CCTGGCCTAG CTGGGCGGGG GGACCCAAAG GCTATGGCAC AACTGAGAGT TCCACAACTG 
GGACCTCGGG CACCAAGTGC CACAGGAAAG GGTCCTAAGG AACTGGACAC CAGAAGTTTA 
AAGGAAGAAA ATTTTATAGC ATCTATTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTAATCAAAC CTGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC GTCTAGTTCC 
CAGAGGACTG ATCAAGACTC TGTCATCGGC GTGAGTCCAG CTGTTATGAT CCGCAGCTCA 
AGTCAGGATT CTGAAGTTAG CACCGTGAGT AATAGCTCTG GAGAGACCCT TGGAGCTGAC 
AGTGACTTGA GCAGCAATGC AGGTGATGGA CCAGGTGGCG AGGGCAGTGT TCACCTGGCA 
AGCTCTCGGG GCACTTTGTC TGATAGTGAA ATTGAGACCA ACTCTGCCAC AAGCACCATC 
TTTGGTAAAG CCCACAGCTT GAAGCCAAGC ATAAAGGAGA AGCTGGCAGG CAGCCCCATT 
CGTACTTCTG AAGATGTGAG CCAGCGAGTC TATCTCTATG AGGGACTCCT AGGAAGGGAC 
AAAGGATCCA TGTGGGACCA GTTAGAGGAT GCAGCTATGG AGACCTTTTC TATAAGCAAA 
GAGCGTTCTA CTTTATGGGA CCAAATGCAA TTCTGGGAAG ATGCCTTCTT AGATGCTGTG 
ATGTTGGAGA GAGAAGGGAT GGGTATGGAC CAGGGTCCCC AGGAAATGAT CGACAGGTAC 
CTGTCCCTTG GAGAACATGA CCGGAAGCGC CTGGAAGATG ATGAAGATCG CTTGTTGGCC 
ACACTTCTGC ACAACCTCAT CTCCTACATG CTGCTGATGA AGGTAAATAA GAATGACATC 
CGCAAGAAGG TGAGGCGCCT AATGGGAAAG TCGCACATTG GGCTTGTGTA CAGCCAGCAA 
ATCAATGAGG TGCTTGATCA GCTGGCGAAC CTGAATGGAC GCGATCTCTC TATCTGGTCC 
AGTGGCAGCC GGCACATGAA GAAGCAGACA TTTGTGGTAC ATGCAGGGAC AGATACAAAC 
GGAGATATCT TTTTCATGGA GGTGTGCGAT GACTGTGTGG TGTTGCGTAG TAACATCGGA 
ACAGTGTATG AGCGCTGGTG GTACGAGAAG CTCATCAACA TGACCTACTG TCCCAAGACG 
AAGGTGTTGT GCTTGTGGCG TAGAAATGGC TCTGAGACCC AGCTCAACAA GTTCTATACT 
AAAAAGTGTC GGGAGCTGTA CTACTGTGTG AAGGACAGCA TGGAGCGCGC TGCCGCCCGA 
CAGCAAAGCA TCAAACCCGG ACCTGAATTG GGTGGCGAGT TCCCTGTGCA GGACCTGAAG 
ACTGGTGAGG GTGGCCTGCT GCAGGTGACC CTGGAAGGGA TCAACCTCAA ATTCATGCAC 
AATCAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTTCG AGGCGTCTTT 
GTCCTGGAGG AATTTGTTCC TGAAATTAAA GAAGTGGTGA GCCACAAGTA CAAGACACCA 
ATGGCCCACG AAATCTGCTA CTCCGTATTA TGTCTCTTCT CGTACGTGGC TGCAGTTCAT 
AGCAGTGAGG AAGATCTCAG AACCCCGCCC CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376611.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363540.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 45, mRNA.

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CloneID	OHu109190
Clone ID Related Accession (Same CDS sequence)	NM_001376611.1
Accession Version	NM_001376611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 36
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.159482.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGAAACAGGA GGGCGTTAGT GGATCAGAAG 
TCATCTGTCA TTAAACACAG CCCAACAGTG AAAAGAGAAC CTCCATCACC CCAGGGTCGA 
TCCAGCAATT CTAGTGAGAA CCAGCAGTTC CTGAAGGAGG TGGTGCACAG CGTGCTGGAC 
GGCCAGGGAG TTGGCTGGCT CAACATGAAA AAGGTGCGCC GGCTGCTGGA GAGCGAGCAG 
CTGCGAGTCT TTGTCCTGAG CAAGCTGAAC CGCATGGTGC AGTCAGAGGA CGATGCCCGG 
CAGGACATCA TCCCGGATGT GGAGATCAGT CGGAAGGTGT ACAAGGGAAT GTTAGACCTC 
CTCAAGTGTA CAGTCCTCAG CTTGGAGCAG TCCTATGCCC ACGCGGGTCT GGGTGGCATG 
GCCAGCATCT TTGGGCTTTT GGAGATTGCC CAGACCCACT ACTATAGTAA AGAACCAGAC 
AAGCGGAAGA GAAGTCCAAC AGAAAGTGTA AATACCCCAG TTGGCAAGGA TCCTGGCCTA 
GCTGGGCGGG GGGACCCAAA GGCTATGGCA CAACTGAGAG TTCCACAACT GGGACCTCGG 
GCACCAAGTG CCACAGGAAA GGGTCCTAAG GAACTGGACA CCAGAAGTTT AAAGGAAGAA 
AATTTTATAG CATCTATTGG GCCTGAAGTA ATCAAACCTG TCTTTGACCT TGGTGAGACA 
GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC AGTGGTGTGA GCCTGACGTC TAGTTCCCAG 
AGGACTGATC AAGACTCTGT CATCGGCGTG AGTCCAGCTG TTATGATCCG CAGCTCAAGT 
CAGGATTCTG AAGTTAGCAC CGTGGTGAGT AATAGCTCTG GAGAGACCCT TGGAGCTGAC 
AGTGACTTGA GCAGCAATGC AGGTGATGGA CCAGGTGGCG AGGGCAGTGT TCACCTGGCA 
AGCTCTCGGG GCACTTTGTC TGATAGTGAA ATTGAGACCA ACTCTGCCAC AAGCACCATC 
TTTGGTAAAG CCCACAGCTT GAAGCCAAGC ATAAAGGAGA AGCTGGCAGG CAGCCCCATT 
CGTACTTCTG AAGATGTGAG CCAGCGAGTC TATCTCTATG AGGGACTCCT AGGCAAAGAG 
CGTTCTACTT TATGGGACCA AATGCAATTC TGGGAAGATG CCTTCTTAGA TGCTGTGATG 
TTGGAGAGAG AAGGGATGGG TATGGACCAG GGTCCCCAGG AAATGATCGA CAGGTACCTG 
TCCCTTGGAG AACATGACCG GAAGCGCCTG GAAGATGATG AAGATCGCTT GTTGGCCACA 
CTTCTGCACA ACCTCATCTC CTACATGCTG CTGATGAAGG TAAATAAGAA TGACATCCGC 
AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG CACATTGGGC TTGTGTACAG CCAGCAAATC 
AATGAGGTGC TTGATCAGCT GGCGAACCTG AATGGACGCG ATCTCTCTAT CTGGTCCAGT 
GGCAGCCGGC ACATGAAGAA GCAGACATTT GTGGTACATG CAGGGACAGA TACAAACGGA 
GATATCTTTT TCATGGAGGT GTGCGATGAC TGTGTGGTGT TGCGTAGTAA CATCGGAACA 
GTGTATGAGC GCTGGTGGTA CGAGAAGCTC ATCAACATGA CCTACTGTCC CAAGACGAAG 
GTGTTGTGCT TGTGGCGTAG AAATGGCTCT GAGACCCAGC TCAACAAGTT CTATACTAAA 
AAGTGTCGGG AGCTGTACTA CTGTGTGAAG GACAGCATGG AGCGCGCTGC CGCCCGACAG 
CAAAGCATCA AACCCGGACC TGAATTGGGT GGCGAGTTCC CTGTGCAGGA CCTGAAGACT 
GGTGAGGGTG GCCTGCTGCA GGTGACCCTG GAAGGGATCA ACCTCAAATT CATGCACAAT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTTCGAGG CGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AATTAAAGAA GTGGTGAGCC ACAAGTACAA GACACCAATG 
GCCCACGAAA TCTGCTACTC CGTATTATGT CTCTTCTCGT ACGTGGCTGC AGTTCATAGC 
AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363540.1
CDS	203..4867
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFARNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376611.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376611.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGAAACAGGA GGGCGTTAGT GGATCAGAAG 
TCATCTGTCA TTAAACACAG CCCAACAGTG AAAAGAGAAC CTCCATCACC CCAGGGTCGA 
TCCAGCAATT CTAGTGAGAA CCAGCAGTTC CTGAAGGAGG TGGTGCACAG CGTGCTGGAC 
GGCCAGGGAG TTGGCTGGCT CAACATGAAA AAGGTGCGCC GGCTGCTGGA GAGCGAGCAG 
CTGCGAGTCT TTGTCCTGAG CAAGCTGAAC CGCATGGTGC AGTCAGAGGA CGATGCCCGG 
CAGGACATCA TCCCGGATGT GGAGATCAGT CGGAAGGTGT ACAAGGGAAT GTTAGACCTC 
CTCAAGTGTA CAGTCCTCAG CTTGGAGCAG TCCTATGCCC ACGCGGGTCT GGGTGGCATG 
GCCAGCATCT TTGGGCTTTT GGAGATTGCC CAGACCCACT ACTATAGTAA AGAACCAGAC 
AAGCGGAAGA GAAGTCCAAC AGAAAGTGTA AATACCCCAG TTGGCAAGGA TCCTGGCCTA 
GCTGGGCGGG GGGACCCAAA GGCTATGGCA CAACTGAGAG TTCCACAACT GGGACCTCGG 
GCACCAAGTG CCACAGGAAA GGGTCCTAAG GAACTGGACA CCAGAAGTTT AAAGGAAGAA 
AATTTTATAG CATCTATTGG GCCTGAAGTA ATCAAACCTG TCTTTGACCT TGGTGAGACA 
GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC AGTGGTGTGA GCCTGACGTC TAGTTCCCAG 
AGGACTGATC AAGACTCTGT CATCGGCGTG AGTCCAGCTG TTATGATCCG CAGCTCAAGT 
CAGGATTCTG AAGTTAGCAC CGTGGTGAGT AATAGCTCTG GAGAGACCCT TGGAGCTGAC 
AGTGACTTGA GCAGCAATGC AGGTGATGGA CCAGGTGGCG AGGGCAGTGT TCACCTGGCA 
AGCTCTCGGG GCACTTTGTC TGATAGTGAA ATTGAGACCA ACTCTGCCAC AAGCACCATC 
TTTGGTAAAG CCCACAGCTT GAAGCCAAGC ATAAAGGAGA AGCTGGCAGG CAGCCCCATT 
CGTACTTCTG AAGATGTGAG CCAGCGAGTC TATCTCTATG AGGGACTCCT AGGCAAAGAG 
CGTTCTACTT TATGGGACCA AATGCAATTC TGGGAAGATG CCTTCTTAGA TGCTGTGATG 
TTGGAGAGAG AAGGGATGGG TATGGACCAG GGTCCCCAGG AAATGATCGA CAGGTACCTG 
TCCCTTGGAG AACATGACCG GAAGCGCCTG GAAGATGATG AAGATCGCTT GTTGGCCACA 
CTTCTGCACA ACCTCATCTC CTACATGCTG CTGATGAAGG TAAATAAGAA TGACATCCGC 
AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG CACATTGGGC TTGTGTACAG CCAGCAAATC 
AATGAGGTGC TTGATCAGCT GGCGAACCTG AATGGACGCG ATCTCTCTAT CTGGTCCAGT 
GGCAGCCGGC ACATGAAGAA GCAGACATTT GTGGTACATG CAGGGACAGA TACAAACGGA 
GATATCTTTT TCATGGAGGT GTGCGATGAC TGTGTGGTGT TGCGTAGTAA CATCGGAACA 
GTGTATGAGC GCTGGTGGTA CGAGAAGCTC ATCAACATGA CCTACTGTCC CAAGACGAAG 
GTGTTGTGCT TGTGGCGTAG AAATGGCTCT GAGACCCAGC TCAACAAGTT CTATACTAAA 
AAGTGTCGGG AGCTGTACTA CTGTGTGAAG GACAGCATGG AGCGCGCTGC CGCCCGACAG 
CAAAGCATCA AACCCGGACC TGAATTGGGT GGCGAGTTCC CTGTGCAGGA CCTGAAGACT 
GGTGAGGGTG GCCTGCTGCA GGTGACCCTG GAAGGGATCA ACCTCAAATT CATGCACAAT 
CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTTCGAGG CGTCTTTGTC 
CTGGAGGAAT TTGTTCCTGA AATTAAAGAA GTGGTGAGCC ACAAGTACAA GACACCAATG 
GCCCACGAAA TCTGCTACTC CGTATTATGT CTCTTCTCGT ACGTGGCTGC AGTTCATAGC 
AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376634.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363563.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 68, mRNA.

Price & Availability↑

CloneID	OHu109194
Clone ID Related Accession (Same CDS sequence)	NM_001376634.1
Accession Version	NM_001376634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 50
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.107000.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363563.1
CDS	177..4745
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376634.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 68, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376634.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376612.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363541.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 46, mRNA.

Price & Availability↑

CloneID	OHu109197
Clone ID Related Accession (Same CDS sequence)	NM_001376612.1
Accession Version	NM_001376612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 37
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2800155.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGGTGTGCGA TGACTGTGTG GTGTTGCGTA GTAACATCGG AACAGTGTAT 
GAGCGCTGGT GGTACGAGAA GCTCATCAAC ATGACCTACT GTCCCAAGAC GAAGGTGTTG 
TGCTTGTGGC GTAGAAATGG CTCTGAGACC CAGCTCAACA AGTTCTATAC TAAAAAGTGT 
CGGGAGCTGT ACTACTGTGT GAAGGACAGC ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC 
ATCAAACCCG GACCTGAATT GGGTGGCGAG TTCCCTGTGC AGGACCTGAA GACTGGTGAG 
GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG ATCAACCTCA AATTCATGCA CAATCAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTTC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC AATGGCCCAC 
GAAATCTGCT ACTCCGTATT ATGTCTCTTC TCGTACGTGG CTGCAGTTCA TAGCAGTGAG 
GAAGATCTCA GAACCCCGCC CCGGCCTGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363541.1
CDS	203..4861
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376612.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 46, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376612.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGGTGTGCGA TGACTGTGTG GTGTTGCGTA GTAACATCGG AACAGTGTAT 
GAGCGCTGGT GGTACGAGAA GCTCATCAAC ATGACCTACT GTCCCAAGAC GAAGGTGTTG 
TGCTTGTGGC GTAGAAATGG CTCTGAGACC CAGCTCAACA AGTTCTATAC TAAAAAGTGT 
CGGGAGCTGT ACTACTGTGT GAAGGACAGC ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC 
ATCAAACCCG GACCTGAATT GGGTGGCGAG TTCCCTGTGC AGGACCTGAA GACTGGTGAG 
GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG ATCAACCTCA AATTCATGCA CAATCAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTTC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC AATGGCCCAC 
GAAATCTGCT ACTCCGTATT ATGTCTCTTC TCGTACGTGG CTGCAGTTCA TAGCAGTGAG 
GAAGATCTCA GAACCCCGCC CCGGCCTGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376574.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363503.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 14, mRNA.

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CloneID	OHu45059
Clone ID Related Accession (Same CDS sequence)	NM_001376574.1
Accession Version	NM_001376574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.53659.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGAAGGG ACAAAGGATC CATGTGGGAC 
CAGTTAGAGG ATGCAGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AATTCTGGGA AGATGCCTTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT TGGAGAACAT 
GACCGGAAGC GCCTGGAAGA TGATGAAGAT CGCTTGTTGG CCACACTTCT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGCAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCGCACAT TGGGCTTGTG TACAGCCAGC AAATCAATGA GGTGCTTGAT 
CAGCTGGCGA ACCTGAATGG ACGCGATCTC TCTATCTGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTTGTGGT ACATGCAGGG ACAGATACAA ACGGAGATAT CTTTTTCATG 
GAGGTGTGCG ATGACTGTGT GGTGTTGCGT AGTAACATCG GAACAGTGTA TGAGCGCTGG 
TGGTACGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CGAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC CCAGCTCAAC AAGTTCTATA CTAAAAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGC GCTGCCGCCC GACAGCAAAG CATCAAACCC 
GGACCTGAAT TGGGTGGCGA GTTCCCTGTG CAGGACCTGA AGACTGGTGA GGGTGGCCTG 
CTGCAGGTGA CCCTGGAAGG GATCAACCTC AAATTCATGC ACAATCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTTC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC AATGGCCCAC 
GAAATCTGCT ACTCCGTATT ATGTCTCTTC TCGTACGTGG CTGCAGTTCA TAGCAGTGAG 
GAAGATCTCA GAACCCCGCC CCGGCCTGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363503.1
CDS	177..5075
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376574.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376574.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGAAGGG ACAAAGGATC CATGTGGGAC 
CAGTTAGAGG ATGCAGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AATTCTGGGA AGATGCCTTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT TGGAGAACAT 
GACCGGAAGC GCCTGGAAGA TGATGAAGAT CGCTTGTTGG CCACACTTCT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGCAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCGCACAT TGGGCTTGTG TACAGCCAGC AAATCAATGA GGTGCTTGAT 
CAGCTGGCGA ACCTGAATGG ACGCGATCTC TCTATCTGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTTGTGGT ACATGCAGGG ACAGATACAA ACGGAGATAT CTTTTTCATG 
GAGGTGTGCG ATGACTGTGT GGTGTTGCGT AGTAACATCG GAACAGTGTA TGAGCGCTGG 
TGGTACGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CGAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC CCAGCTCAAC AAGTTCTATA CTAAAAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGC GCTGCCGCCC GACAGCAAAG CATCAAACCC 
GGACCTGAAT TGGGTGGCGA GTTCCCTGTG CAGGACCTGA AGACTGGTGA GGGTGGCCTG 
CTGCAGGTGA CCCTGGAAGG GATCAACCTC AAATTCATGC ACAATCAGGA GCGGAAGGTT 
TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTTC GAGGCGTCTT TGTCCTGGAG 
GAATTTGTTC CTGAAATTAA AGAAGTGGTG AGCCACAAGT ACAAGACACC AATGGCCCAC 
GAAATCTGCT ACTCCGTATT ATGTCTCTTC TCGTACGTGG CTGCAGTTCA TAGCAGTGAG 
GAAGATCTCA GAACCCCGCC CCGGCCTGTC TCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376575.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363504.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 15, mRNA.

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CloneID	OHu45060
Clone ID Related Accession (Same CDS sequence)	NM_001376575.1
Accession Version	NM_001376575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.284604.1, SRR1803617.273309.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTTCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCACGAAATC 
TGCTACTCCG TATTATGTCT CTTCTCGTAC GTGGCTGCAG TTCATAGCAG TGAGGAAGAT 
CTCAGAACCC CGCCCCGGCC TGTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363504.1
CDS	203..5095
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376575.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376575.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTTCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCACGAAATC 
TGCTACTCCG TATTATGTCT CTTCTCGTAC GTGGCTGCAG TTCATAGCAG TGAGGAAGAT 
CTCAGAACCC CGCCCCGGCC TGTCTCTAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376616.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363545.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 50, mRNA.

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CloneID	OHu45075
Clone ID Related Accession (Same CDS sequence)	NM_001376616.1 , XM_005253203.3
Accession Version	NM_001376616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2834178.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363545.1
CDS	333..4970
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376616.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 50, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376616.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376621.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363550.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 55, mRNA.

Price & Availability↑

CloneID	OHu45076
Clone ID Related Accession (Same CDS sequence)	NM_001376621.1 , NM_001376619.1 , XM_005253204.3 , XM_005253204.2
Accession Version	NM_001376621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363550.1
CDS	301..4926
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376621.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 55, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376621.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376619.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363548.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 53, mRNA.

Price & Availability↑

CloneID	OHu45076
Clone ID Related Accession (Same CDS sequence)	NM_001376621.1 , NM_001376619.1 , XM_005253204.3 , XM_005253204.2
Accession Version	NM_001376619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363548.1
CDS	333..4958
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376619.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 53, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376619.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376594.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363523.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 28, mRNA.

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CloneID	OHu92630
Clone ID Related Accession (Same CDS sequence)	NM_001376594.1 , NM_001376593.1 , XM_017018485.2 , XM_017018484.1 , XM_017018485.1
Accession Version	NM_001376594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 24
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_017018485.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.135277.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363523.1
CDS	177..4931
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376594.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376594.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376593.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363522.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 27, mRNA.

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CloneID	OHu92630
Clone ID Related Accession (Same CDS sequence)	NM_001376594.1 , NM_001376593.1 , XM_017018485.2 , XM_017018484.1 , XM_017018485.1
Accession Version	NM_001376593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 24
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_017018484.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363522.1
CDS	203..4957
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001376593.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376593.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376600.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363529.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 34, mRNA.

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CloneID	OHu92633
Clone ID Related Accession (Same CDS sequence)	NM_001376600.1 , XM_017018488.1 , XM_017018489.1
Accession Version	NM_001376600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 27
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_017018489.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.37454.1, SRR1803611.172792.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363529.1
CDS	177..4910
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376600.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376600.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376605.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363534.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 39, mRNA.

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CloneID	OHu92634
Clone ID Related Accession (Same CDS sequence)	NM_001376605.1
Accession Version	NM_001376605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 30
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_017018490.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.263563.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGAAGGG ACAAAGGATC CATGTGGGAC 
CAGTTAGAGG ATGCAGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AATTCTGGGA AGATGCCTTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT TGGAGAACAT 
GACCGGAAGC GCCTGGAAGA TGATGAAGAT CGCTTGTTGG CCACACTTCT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGCAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCGCACAT TGGGCTTGTG TACAGCCAGC AAATCAATGA GGTGCTTGAT 
CAGCTGGCGA ACCTGAATGG ACGCGATCTC TCTATCTGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTTGTGGT ACATGCAGGG ACAGATACAA ACGGAGATAT CTTTTTCATG 
GAGGTGTGCG ATGACTGTGT GGTGTTGCGT AGTAACATCG GAACAGTGTA TGAGCGCTGG 
TGGTACGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CGAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC CCAGCTCAAC AAGTTCTATA CTAAAAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGC GCTGCCGCCC GACAGCAAAG CATCAAACCC 
GGACCTGAAT TGGGTGGCGA GTTCCCTGTG CAGGACCTGA AGACTGGTGA GGGTGGCCTG 
CTGCAGGTGA CCCTGGAAGG GATCAACCTC AAATTCATGC ACAATCAGTT CCTGAAATTA 
AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363534.1
CDS	203..4894
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376605.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376605.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGAAGGG ACAAAGGATC CATGTGGGAC 
CAGTTAGAGG ATGCAGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AATTCTGGGA AGATGCCTTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT TGGAGAACAT 
GACCGGAAGC GCCTGGAAGA TGATGAAGAT CGCTTGTTGG CCACACTTCT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGCAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCGCACAT TGGGCTTGTG TACAGCCAGC AAATCAATGA GGTGCTTGAT 
CAGCTGGCGA ACCTGAATGG ACGCGATCTC TCTATCTGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTTGTGGT ACATGCAGGG ACAGATACAA ACGGAGATAT CTTTTTCATG 
GAGGTGTGCG ATGACTGTGT GGTGTTGCGT AGTAACATCG GAACAGTGTA TGAGCGCTGG 
TGGTACGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CGAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC CCAGCTCAAC AAGTTCTATA CTAAAAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGC GCTGCCGCCC GACAGCAAAG CATCAAACCC 
GGACCTGAAT TGGGTGGCGA GTTCCCTGTG CAGGACCTGA AGACTGGTGA GGGTGGCCTG 
CTGCAGGTGA CCCTGGAAGG GATCAACCTC AAATTCATGC ACAATCAGTT CCTGAAATTA 
AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376609.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363538.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 43, mRNA.

Price & Availability↑

CloneID	OHu92636
Clone ID Related Accession (Same CDS sequence)	NM_001376609.1
Accession Version	NM_001376609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 34
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_017018492.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.21507.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363538.1
CDS	203..4882
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376609.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376609.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376622.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363551.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 56, mRNA.

Price & Availability↑

CloneID	OHu92640
Clone ID Related Accession (Same CDS sequence)	NM_001376622.1
Accession Version	NM_001376622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 41
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.121241.1, SRR1660803.23313.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363551.1
CDS	177..4796
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376622.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 56, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376622.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376629.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363558.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 63, mRNA.

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CloneID	OHu92641
Clone ID Related Accession (Same CDS sequence)	XM_017018499.1 , NM_001376629.1
Accession Version	NM_001376629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 46
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_017018498.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.63532.1, SRR1803611.122154.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GTTCCTGAAA TTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363558.1
CDS	203..4807
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376629.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 63, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376629.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GTTCCTGAAA TTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376636.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363565.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 70, mRNA.

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CloneID	OHu92643
Clone ID Related Accession (Same CDS sequence)	XM_017018502.1 , NM_001376636.1
Accession Version	NM_001376636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 52
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.231904.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363565.1
CDS	333..4895
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376636.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 70, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376636.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376638.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363567.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 72, mRNA.

Price & Availability↑

CloneID	OHu92644
Clone ID Related Accession (Same CDS sequence)	XM_017018504.1 , NM_001376638.1 , XM_017018503.1 , NM_001376639.1
Accession Version	NM_001376638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.262376.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363567.1
CDS	333..4892
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376638.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 72, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376638.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376639.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363568.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 73, mRNA.

Price & Availability↑

CloneID	OHu92644
Clone ID Related Accession (Same CDS sequence)	XM_017018504.1 , NM_001376638.1 , XM_017018503.1 , NM_001376639.1
Accession Version	NM_001376639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.108695.1, SRR1803617.258786.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363568.1
CDS	203..4762
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376639.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 73, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376639.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376643.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363572.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 77, mRNA.

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CloneID	OHu92645
Clone ID Related Accession (Same CDS sequence)	XM_017018505.2 , NM_001376643.1 , NM_001376642.1
Accession Version	NM_001376643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 56
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_017018505.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.13668.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363572.1
CDS	203..4753
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376643.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 77, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376643.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001376642.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363571.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 76, mRNA.

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CloneID	OHu92645
Clone ID Related Accession (Same CDS sequence)	XM_017018505.2 , NM_001376643.1 , NM_001376642.1
Accession Version	NM_001376642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 56
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Nov 18, 2019 this sequence version replaced XM_017018506.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.31151.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363571.1
CDS	177..4727
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_001376642.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 76, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376642.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448749.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304517.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

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CloneID	OHu103649
Clone ID Related Accession (Same CDS sequence)	XM_024448749.1
Accession Version	XM_024448749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ACGAAATCTG CTACTCCGTA 
TTATGTCTCT TCTCGTACGT GGCTGCAGTT CATAGCAGTG AGGAAGATCT CAGAACCCCG 
CCCCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304517.1
CDS	216..5036
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024448749.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448749.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ACGAAATCTG CTACTCCGTA 
TTATGTCTCT TCTCGTACGT GGCTGCAGTT CATAGCAGTG AGGAAGATCT CAGAACCCCG 
CCCCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448750.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304518.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu103650
Clone ID Related Accession (Same CDS sequence)	XM_024448750.1
Accession Version	XM_024448750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCAATGGCC CACGAAATCT GCTACTCCGT ATTATGTCTC 
TTCTCGTACG TGGCTGCAGT TCATAGCAGT GAGGAAGATC TCAGAACCCC GCCCCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304518.1
CDS	203..5014
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024448750.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448750.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCAATGGCC CACGAAATCT GCTACTCCGT ATTATGTCTC 
TTCTCGTACG TGGCTGCAGT TCATAGCAGT GAGGAAGATC TCAGAACCCC GCCCCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448752.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304520.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OHu103651
Clone ID Related Accession (Same CDS sequence)	XM_024448752.1
Accession Version	XM_024448752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304520.1
CDS	194..4876
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_024448752.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448752.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448753.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304521.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

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CloneID	OHu103652
Clone ID Related Accession (Same CDS sequence)	NM_001376624.1 , NM_001376625.1 , XM_024448753.1 , XM_024448754.1
Accession Version	XM_024448753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304521.1
CDS	194..4810
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_024448753.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448753.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448754.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304522.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X33, mRNA.

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CloneID	OHu103652
Clone ID Related Accession (Same CDS sequence)	NM_001376624.1 , NM_001376625.1 , XM_024448753.1 , XM_024448754.1
Accession Version	XM_024448754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304522.1
CDS	185..4801
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_024448754.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448754.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGTTCCTGA AATTAAAGAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448755.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304523.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X34, mRNA.

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CloneID	OHu103653
Clone ID Related Accession (Same CDS sequence)	XM_024448755.1 , NM_001376628.1
Accession Version	XM_024448755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304523.1
CDS	194..4807
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_024448755.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448755.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448746.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304514.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu45056
Clone ID Related Accession (Same CDS sequence)	XM_024448746.1
Accession Version	XM_024448746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTT CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA CGAAATCTGC 
TACTCCGTAT TATGTCTCTT CTCGTACGTG GCTGCAGTTC ATAGCAGTGA GGAAGATCTC 
AGAACCCCGC CCCGGCCTGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304514.1
CDS	216..5165
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024448746.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448746.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGG AGCGGAAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTT CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA CGAAATCTGC 
TACTCCGTAT TATGTCTCTT CTCGTACGTG GCTGCAGTTC ATAGCAGTGA GGAAGATCTC 
AGAACCCCGC CCCGGCCTGT CTCTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448747.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304515.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu45058
Clone ID Related Accession (Same CDS sequence)	XM_024448747.1
Accession Version	XM_024448747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ACGAAATCTG CTACTCCGTA 
TTATGTCTCT TCTCGTACGT GGCTGCAGTT CATAGCAGTG AGGAAGATCT CAGAACCCCG 
CCCCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304515.1
CDS	191..5131
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024448747.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448747.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT TCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ACGAAATCTG CTACTCCGTA 
TTATGTCTCT TCTCGTACGT GGCTGCAGTT CATAGCAGTG AGGAAGATCT CAGAACCCCG 
CCCCGGCCTG TCTCTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448751.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304519.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

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CloneID	OHu45073
Clone ID Related Accession (Same CDS sequence)	XM_024448751.1
Accession Version	XM_024448751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304519.1
CDS	216..4901
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_024448751.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448751.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_024448748.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304516.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

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CloneID	OHu61992
Clone ID Related Accession (Same CDS sequence)	XM_024448748.1
Accession Version	XM_024448748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304516.1
CDS	216..5039
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_024448748.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448748.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TTAGCACCGT GGTGAGTAAT 
AGCTCTGGAG AGACCCTTGG AGCTGACAGT GACTTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGCGAGG GCAGTGTTCA CCTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAACT CTGCCACAAG CACCATCTTT GGTAAAGCCC ACAGCTTGAA GCCAAGCATA 
AAGGAGAAGC TGGCAGGCAG CCCCATTCGT ACTTCTGAAG ATGTGAGCCA GCGAGTCTAT 
CTCTATGAGG GACTCCTAGG AAGGGACAAA GGATCCATGT GGGACCAGTT AGAGGATGCA 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018478.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873967.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

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CloneID	OHu92624
Clone ID Related Accession (Same CDS sequence)	XM_017018478.2 , XM_017018478.1
Accession Version	XM_017018478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCAATGGCC CACGAAATCT GCTACTCCGT ATTATGTCTC 
TTCTCGTACG TGGCTGCAGT TCATAGCAGT GAGGAAGATC TCAGAACCCC GCCCCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873967.1
CDS	194..5125
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018478.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018478.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCAATGGCC CACGAAATCT GCTACTCCGT ATTATGTCTC 
TTCTCGTACG TGGCTGCAGT TCATAGCAGT GAGGAAGATC TCAGAACCCC GCCCCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018478.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873967.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu92624
Clone ID Related Accession (Same CDS sequence)	XM_017018478.2 , XM_017018478.1
Accession Version	XM_017018478.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCAATGGCC CACGAAATCT GCTACTCCGT ATTATGTCTC 
TTCTCGTACG TGGCTGCAGT TCATAGCAGT GAGGAAGATC TCAGAACCCC GCCCCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873967.1
CDS	216..5147
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018478.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018478.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG GTTTTCATAG AGCTGAATCA CATTAAAAAG 
TGCAATACAG TTCGAGGCGT CTTTGTCCTG GAGGAATTTG TTCCTGAAAT TAAAGAAGTG 
GTGAGCCACA AGTACAAGAC ACCAATGGCC CACGAAATCT GCTACTCCGT ATTATGTCTC 
TTCTCGTACG TGGCTGCAGT TCATAGCAGT GAGGAAGATC TCAGAACCCC GCCCCGGCCT 
GTCTCTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018479.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873968.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu92625
Clone ID Related Accession (Same CDS sequence)	XM_017018479.1
Accession Version	XM_017018479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873968.1
CDS	216..5093
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018479.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018479.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018480.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873969.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

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CloneID	OHu92626
Clone ID Related Accession (Same CDS sequence)	XM_017018480.1
Accession Version	XM_017018480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873969.1
CDS	216..5039
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018480.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018480.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018482.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873971.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OHu92628
Clone ID Related Accession (Same CDS sequence)	XM_017018482.1
Accession Version	XM_017018482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873971.1
CDS	216..5030
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018482.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018482.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018483.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873972.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

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CloneID	OHu92629
Clone ID Related Accession (Same CDS sequence)	XM_017018483.1
Accession Version	XM_017018483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA 
GTTCGAGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CACCAATGGC CCACGAAATC TGCTACTCCG TATTATGTCT CTTCTCGTAC 
GTGGCTGCAG TTCATAGCAG TGAGGAAGAT CTCAGAACCC CGCCCCGGCC TGTCTCTAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873972.1
CDS	216..5018
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018483.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018483.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA 
GTTCGAGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CACCAATGGC CCACGAAATC TGCTACTCCG TATTATGTCT CTTCTCGTAC 
GTGGCTGCAG TTCATAGCAG TGAGGAAGAT CTCAGAACCC CGCCCCGGCC TGTCTCTAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018485.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873974.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

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CloneID	OHu92630
Clone ID Related Accession (Same CDS sequence)	NM_001376594.1 , NM_001376593.1 , XM_017018485.2 , XM_017018484.1 , XM_017018485.1
Accession Version	XM_017018485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873974.1
CDS	177..4931
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018485.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018485.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018484.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873973.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu92630
Clone ID Related Accession (Same CDS sequence)	NM_001376594.1 , NM_001376593.1 , XM_017018485.2 , XM_017018484.1 , XM_017018485.1
Accession Version	XM_017018484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873973.1
CDS	216..4970
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018484.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018484.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018485.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873974.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu92630
Clone ID Related Accession (Same CDS sequence)	NM_001376594.1 , NM_001376593.1 , XM_017018485.2 , XM_017018484.1 , XM_017018485.1
Accession Version	XM_017018485.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873974.1
CDS	177..4931
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018485.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018485.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GTTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018487.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873976.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

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CloneID	OHu92632
Clone ID Related Accession (Same CDS sequence)	XM_017018487.1
Accession Version	XM_017018487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA 
GTTCGAGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CACCAATGGC CCACGAAATC TGCTACTCCG TATTATGTCT CTTCTCGTAC 
GTGGCTGCAG TTCATAGCAG TGAGGAAGAT CTCAGAACCC CGCCCCGGCC TGTCTCTAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873976.1
CDS	216..4958
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_017018487.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018487.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA 
GTTCGAGGCG TCTTTGTCCT GGAGGAATTT GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC 
AAGTACAAGA CACCAATGGC CCACGAAATC TGCTACTCCG TATTATGTCT CTTCTCGTAC 
GTGGCTGCAG TTCATAGCAG TGAGGAAGAT CTCAGAACCC CGCCCCGGCC TGTCTCTAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018488.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873977.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

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CloneID	OHu92633
Clone ID Related Accession (Same CDS sequence)	NM_001376600.1 , XM_017018488.1 , XM_017018489.1
Accession Version	XM_017018488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873977.1
CDS	216..4949
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018488.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018488.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018489.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873978.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X22, mRNA.

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CloneID	OHu92633
Clone ID Related Accession (Same CDS sequence)	NM_001376600.1 , XM_017018488.1 , XM_017018489.1
Accession Version	XM_017018489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873978.1
CDS	177..4910
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018489.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018489.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018491.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873980.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OHu92635
Clone ID Related Accession (Same CDS sequence)	XM_017018491.1
Accession Version	XM_017018491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGAAGGGACA AAGGATCCAT GTGGGACCAG 
TTAGAGGATG CAGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873980.1
CDS	172..4860
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018491.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018491.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGAAGGGACA AAGGATCCAT GTGGGACCAG 
TTAGAGGATG CAGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018493.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873982.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OHu92637
Clone ID Related Accession (Same CDS sequence)	XM_017018493.1
Accession Version	XM_017018493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873982.1
CDS	203..4876
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018493.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018493.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT GAGTAATAGC 
TCTGGAGAGA CCCTTGGAGC TGACAGTGAC TTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGCGAGGGCA GTGTTCACCT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAACTCTG CCACAAGCAC CATCTTTGGT AAAGCCCACA GCTTGAAGCC AAGCATAAAG 
GAGAAGCTGG CAGGCAGCCC CATTCGTACT TCTGAAGATG TGAGCCAGCG AGTCTATCTC 
TATGAGGGAC TCCTAGGAAG GGACAAAGGA TCCATGTGGG ACCAGTTAGA GGATGCAGCT 
ATGGAGACCT TTTCTATAAG CAAAGAGCGT TCTACTTTAT GGGACCAAAT GCAATTCTGG 
GAAGATGCCT TCTTAGATGC TGTGATGTTG GAGAGAGAAG GGATGGGTAT GGACCAGGGT 
CCCCAGGAAA TGATCGACAG GTACCTGTCC CTTGGAGAAC ATGACCGGAA GCGCCTGGAA 
GATGATGAAG ATCGCTTGTT GGCCACACTT CTGCACAACC TCATCTCCTA CATGCTGCTG 
ATGAAGGTAA ATAAGAATGA CATCCGCAAG AAGGTGAGGC GCCTAATGGG AAAGTCGCAC 
ATTGGGCTTG TGTACAGCCA GCAAATCAAT GAGGTGCTTG ATCAGCTGGC GAACCTGAAT 
GGACGCGATC TCTCTATCTG GTCCAGTGGC AGCCGGCACA TGAAGAAGCA GACATTTGTG 
GTACATGCAG GGACAGATAC AAACGGAGAT ATCTTTTTCA TGGAGGTGTG CGATGACTGT 
GTGGTGTTGC GTAGTAACAT CGGAACAGTG TATGAGCGCT GGTGGTACGA GAAGCTCATC 
AACATGACCT ACTGTCCCAA GACGAAGGTG TTGTGCTTGT GGCGTAGAAA TGGCTCTGAG 
ACCCAGCTCA ACAAGTTCTA TACTAAAAAG TGTCGGGAGC TGTACTACTG TGTGAAGGAC 
AGCATGGAGC GCGCTGCCGC CCGACAGCAA AGCATCAAAC CCGGACCTGA ATTGGGTGGC 
GAGTTCCCTG TGCAGGACCT GAAGACTGGT GAGGGTGGCC TGCTGCAGGT GACCCTGGAA 
GGGATCAACC TCAAATTCAT GCACAATCAG TTCCTGAAAT TAAAGAAGTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018494.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873983.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

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CloneID	OHu92638
Clone ID Related Accession (Same CDS sequence)	XM_017018494.1
Accession Version	XM_017018494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGAAGGG ACAAAGGATC CATGTGGGAC 
CAGTTAGAGG ATGCAGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AATTCTGGGA AGATGCCTTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT TGGAGAACAT 
GACCGGAAGC GCCTGGAAGA TGATGAAGAT CGCTTGTTGG CCACACTTCT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGCAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCGCACAT TGGGCTTGTG TACAGCCAGC AAATCAATGA GGTGCTTGAT 
CAGCTGGCGA ACCTGAATGG ACGCGATCTC TCTATCTGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTTGTGGT ACATGCAGGG ACAGATACAA ACGGAGATAT CTTTTTCATG 
GAGGTGTGCG ATGACTGTGT GGTGTTGCGT AGTAACATCG GAACAGTGTA TGAGCGCTGG 
TGGTACGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CGAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC CCAGCTCAAC AAGTTCTATA CTAAAAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGC GCTGCCGCCC GACAGCAAAG CATCAAACCC 
GGACCTGAAT TGGGTGGCGA GTTCCCTGTG CAGGACCTGA AGACTGGTGA GGGTGGCCTG 
CTGCAGGTGA CCCTGGAAGG GATCAACCTC AAATTCATGC ACAATCAGTT CCTGAAATTA 
AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873983.1
CDS	216..4847
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018494.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018494.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGAAGGG ACAAAGGATC CATGTGGGAC 
CAGTTAGAGG ATGCAGCTAT GGAGACCTTT TCTATAAGCA AAGAGCGTTC TACTTTATGG 
GACCAAATGC AATTCTGGGA AGATGCCTTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT TGGAGAACAT 
GACCGGAAGC GCCTGGAAGA TGATGAAGAT CGCTTGTTGG CCACACTTCT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGCAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCGCACAT TGGGCTTGTG TACAGCCAGC AAATCAATGA GGTGCTTGAT 
CAGCTGGCGA ACCTGAATGG ACGCGATCTC TCTATCTGGT CCAGTGGCAG CCGGCACATG 
AAGAAGCAGA CATTTGTGGT ACATGCAGGG ACAGATACAA ACGGAGATAT CTTTTTCATG 
GAGGTGTGCG ATGACTGTGT GGTGTTGCGT AGTAACATCG GAACAGTGTA TGAGCGCTGG 
TGGTACGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CGAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC CCAGCTCAAC AAGTTCTATA CTAAAAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGC GCTGCCGCCC GACAGCAAAG CATCAAACCC 
GGACCTGAAT TGGGTGGCGA GTTCCCTGTG CAGGACCTGA AGACTGGTGA GGGTGGCCTG 
CTGCAGGTGA CCCTGGAAGG GATCAACCTC AAATTCATGC ACAATCAGTT CCTGAAATTA 
AAGAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018495.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873984.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

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CloneID	OHu92639
Clone ID Related Accession (Same CDS sequence)	XM_017018495.1
Accession Version	XM_017018495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGAAGGGACA AAGGATCCAT GTGGGACCAG 
TTAGAGGATG CAGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873984.1
CDS	216..4844
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018495.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018495.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGAAGGGACA AAGGATCCAT GTGGGACCAG 
TTAGAGGATG CAGCTATGGA GACCTTTTCT ATAAGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018499.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873988.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X36, mRNA.

Price & Availability↑

CloneID	OHu92641
Clone ID Related Accession (Same CDS sequence)	XM_017018499.1 , NM_001376629.1
Accession Version	XM_017018499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GTTCCTGAAA TTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873988.1
CDS	185..4789
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018499.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018499.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGAATTGTGG 
AACAAGCACC AGGAAGTGAA AAAGCAAAAA GCTTTGGAAA AACAGAGGCC TGAAGTAATC 
AAACCTGTCT TTGACCTTGG TGAGACAGAG GAGAAAAAGT CCCAGATCAG CGCAGACAGT 
GGTGTGAGCC TGACGTCTAG TTCCCAGAGG ACTGATCAAG ACTCTGTCAT CGGCGTGAGT 
CCAGCTGTTA TGATCCGCAG CTCAAGTCAG GATTCTGAAG TGAGTAATAG CTCTGGAGAG 
ACCCTTGGAG CTGACAGTGA CTTGAGCAGC AATGCAGGTG ATGGACCAGG TGGCGAGGGC 
AGTGTTCACC TGGCAAGCTC TCGGGGCACT TTGTCTGATA GTGAAATTGA GACCAACTCT 
GCCACAAGCA CCATCTTTGG TAAAGCCCAC AGCTTGAAGC CAAGCATAAA GGAGAAGCTG 
GCAGGCAGCC CCATTCGTAC TTCTGAAGAT GTGAGCCAGC GAGTCTATCT CTATGAGGGA 
CTCCTAGGAA GGGACAAAGG ATCCATGTGG GACCAGTTAG AGGATGCAGC TATGGAGACC 
TTTTCTATAA GCAAAGAGCG TTCTACTTTA TGGGACCAAA TGCAATTCTG GGAAGATGCC 
TTCTTAGATG CTGTGATGTT GGAGAGAGAA GGGATGGGTA TGGACCAGGG TCCCCAGGAA 
ATGATCGACA GGTACCTGTC CCTTGGAGAA CATGACCGGA AGCGCCTGGA AGATGATGAA 
GATCGCTTGT TGGCCACACT TCTGCACAAC CTCATCTCCT ACATGCTGCT GATGAAGGTA 
AATAAGAATG ACATCCGCAA GAAGGTGAGG CGCCTAATGG GAAAGTCGCA CATTGGGCTT 
GTGTACAGCC AGCAAATCAA TGAGGTGCTT GATCAGCTGG CGAACCTGAA TGGACGCGAT 
CTCTCTATCT GGTCCAGTGG CAGCCGGCAC ATGAAGAAGC AGACATTTGT GGTACATGCA 
GGGACAGATA CAAACGGAGA TATCTTTTTC ATGGAGGTGT GCGATGACTG TGTGGTGTTG 
CGTAGTAACA TCGGAACAGT GTATGAGCGC TGGTGGTACG AGAAGCTCAT CAACATGACC 
TACTGTCCCA AGACGAAGGT GTTGTGCTTG TGGCGTAGAA ATGGCTCTGA GACCCAGCTC 
AACAAGTTCT ATACTAAAAA GTGTCGGGAG CTGTACTACT GTGTGAAGGA CAGCATGGAG 
CGCGCTGCCG CCCGACAGCA AAGCATCAAA CCCGGACCTG AATTGGGTGG CGAGTTCCCT 
GTGCAGGACC TGAAGACTGG TGAGGGTGGC CTGCTGCAGG TGACCCTGGA AGGGATCAAC 
CTCAAATTCA TGCACAATCA GTTCCTGAAA TTAAAGAAGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018502.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873991.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OHu92643
Clone ID Related Accession (Same CDS sequence)	XM_017018502.1 , NM_001376636.1
Accession Version	XM_017018502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873991.1
CDS	185..4747
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018502.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018502.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018504.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873993.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X34, mRNA.

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CloneID	OHu92644
Clone ID Related Accession (Same CDS sequence)	XM_017018504.1 , NM_001376638.1 , XM_017018503.1 , NM_001376639.1
Accession Version	XM_017018504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873993.1
CDS	185..4744
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018504.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018504.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018503.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873992.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X33, mRNA.

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CloneID	OHu92644
Clone ID Related Accession (Same CDS sequence)	XM_017018504.1 , NM_001376638.1 , XM_017018503.1 , NM_001376639.1
Accession Version	XM_017018503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873992.1
CDS	172..4731
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018503.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018503.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGAAGGGAC AAAGGATCCA TGTGGGACCA GTTAGAGGAT 
GCAGCTATGG AGACCTTTTC TATAAGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018505.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873994.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X38, mRNA.

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CloneID	OHu92645
Clone ID Related Accession (Same CDS sequence)	XM_017018505.2 , NM_001376643.1 , NM_001376642.1
Accession Version	XM_017018505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873994.1
CDS	194..4744
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018505.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018505.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGAAGGGA CAAAGGATCC ATGTGGGACC AGTTAGAGGA TGCAGCTATG 
GAGACCTTTT CTATAAGCAA AGAGCGTTCT ACTTTATGGG ACCAAATGCA ATTCTGGGAA 
GATGCCTTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTT GGAGAACATG ACCGGAAGCG CCTGGAAGAT 
GATGAAGATC GCTTGTTGGC CACACTTCTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGCAAGAAG GTGAGGCGCC TAATGGGAAA GTCGCACATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAG GTGCTTGATC AGCTGGCGAA CCTGAATGGA 
CGCGATCTCT CTATCTGGTC CAGTGGCAGC CGGCACATGA AGAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGATACAAA CGGAGATATC TTTTTCATGG AGGTGTGCGA TGACTGTGTG 
GTGTTGCGTA GTAACATCGG AACAGTGTAT GAGCGCTGGT GGTACGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC GAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACC 
CAGCTCAACA AGTTCTATAC TAAAAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGCG CTGCCGCCCG ACAGCAAAGC ATCAAACCCG GACCTGAATT GGGTGGCGAG 
TTCCCTGTGC AGGACCTGAA GACTGGTGAG GGTGGCCTGC TGCAGGTGAC CCTGGAAGGG 
ATCAACCTCA AATTCATGCA CAATCAGTTC CTGAAATTAA AGAAGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_017018508.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873997.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X38, mRNA.

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CloneID	OHu92647
Clone ID Related Accession (Same CDS sequence)	XM_017018508.1
Accession Version	XM_017018508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873997.1
CDS	216..4718
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_017018508.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018508.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGAAGG GACAAAGGAT CCATGTGGGA CCAGTTAGAG 
GATGCAGCTA TGGAGACCTT TTCTATAAGC AAAGAGCGTT CTACTTTATG GGACCAAATG 
CAATTCTGGG AAGATGCCTT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TTGGAGAACA TGACCGGAAG 
CGCCTGGAAG ATGATGAAGA TCGCTTGTTG GCCACACTTC TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGCAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCGCACA TTGGGCTTGT GTACAGCCAG CAAATCAATG AGGTGCTTGA TCAGCTGGCG 
AACCTGAATG GACGCGATCT CTCTATCTGG TCCAGTGGCA GCCGGCACAT GAAGAAGCAG 
ACATTTGTGG TACATGCAGG GACAGATACA AACGGAGATA TCTTTTTCAT GGAGGTGTGC 
GATGACTGTG TGGTGTTGCG TAGTAACATC GGAACAGTGT ATGAGCGCTG GTGGTACGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACGAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CCCAGCTCAA CAAGTTCTAT ACTAAAAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG CGCTGCCGCC CGACAGCAAA GCATCAAACC CGGACCTGAA 
TTGGGTGGCG AGTTCCCTGT GCAGGACCTG AAGACTGGTG AGGGTGGCCT GCTGCAGGTG 
ACCCTGGAAG GGATCAACCT CAAATTCATG CACAATCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_130470.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_569826.2 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05881
Clone ID Related Accession (Same CDS sequence)	NM_130470.2
Accession Version	NM_130470.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187733.1, U77352.1, AC018410.24, AF440103.1 and AF440101.1. On Oct 21, 2008 this sequence version replaced NM_130470.1. Transcript Variant: This variant (1) has a different exon 1 sequence and lacks exons 16, 21 and 26, as compared to transcript variant 4. The resulting isoform (a) is shorter than isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U77352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGCTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569826.2
CDS	358..5124
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_130470.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130470.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGCTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_130471.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_569827.2 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 2, mRNA.

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CloneID	OHu05883
Clone ID Related Accession (Same CDS sequence)	NM_130471.2
Accession Version	NM_130471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU077339.1, AF440102.1 and U77352.1. On Oct 21, 2008 this sequence version replaced NM_130471.1. Transcript Variant: This variant (2) lacks exons 21, 26, and a portion of exon 13 as compared to transcript variant 4. The resulting isoform (b) is missing regions of 43 aa, 18 aa, and 21 aa but has identical N- and C-termini as those of isoform (d) encoded by transcript variant 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF440102.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGCTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569827.2
CDS	192..4889
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_130471.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130471.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGCTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_130474.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_569830.2 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 6, mRNA.

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CloneID	OHu05887
Clone ID Related Accession (Same CDS sequence)	NM_130474.2
Accession Version	NM_130474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU077339.1, AF440434.1 and U77352.1. On Oct 21, 2008 this sequence version replaced NM_130474.1. Transcript Variant: This variant (6) lacks exons 16, 21, 26, 34, and part of exon 13 but maintains the same reading frame up through exon 33 as compared to transcript variant 4. The resulting isoform (f) is missing regions of 43 aa, 20 aa, 18 aa, and 21 aa and has a different carboxyl terminus as compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF440434.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGCTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569830.2
CDS	192..4631
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_130474.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130474.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGCTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_003682.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003673.3 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 4, mRNA.

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CloneID	OHu18382
Clone ID Related Accession (Same CDS sequence)	NM_003682.3
Accession Version	NM_003682.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU077339.1, AF440100.1 and U77352.1. This sequence is a reference standard in the RefSeqGene project. On Oct 21, 2008 this sequence version replaced NM_003682.2. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (d). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF440100.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG CTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003673.3
CDS	192..5135
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_003682.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003682.3

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG CTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_130476.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_569832.2 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu30554
Clone ID Related Accession (Same CDS sequence)	NM_130476.2
Accession Version	NM_130476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA759613.1, U44953.1, AF440100.1 and U77352.1. On Oct 17, 2008 this sequence version replaced NM_130476.1. Transcript Variant: This variant (8) has a different exon 1 sequence, lacks exons 16, 21 and 26, and uses an alternate in-frame splice site in exon 23, as compared to transcript variant 4. The resulting isoform (h) is shorter than isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U44953.1, SRR1803611.170306.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG CTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569832.2
CDS	216..4979
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_130476.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130476.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGAGTA ATAGCTCTGG AGAGACCCTT GGAGCTGACA GTGACTTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGCGA GGGCAGTGTT CACCTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA CTCTGCCACA AGCACCATCT TTGGTAAAGC CCACAGCTTG 
AAGCCAAGCA TAAAGGAGAA GCTGGCAGGC AGCCCCATTC GTACTTCTGA AGATGTGAGC 
CAGCGAGTCT ATCTCTATGA GGGACTCCTA GGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG CTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_130475.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_569831.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu31353
Clone ID Related Accession (Same CDS sequence)	NM_130475.2
Accession Version	NM_130475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA759613.1, AB002356.1, AF440100.1 and U77352.1. On Oct 21, 2008 this sequence version replaced NM_130475.1. Transcript Variant: This variant (7) has a different exon 1 sequence and lacks exon 34 as compared to transcript variant 4. The resulting isoform (g) is shorter and has a different carboxyl terminus as compared to the isoform (d) encoded by transcript variant 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002356.1, SRR1660803.185427.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG CTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569831.1
CDS	216..4961
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_130475.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130475.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG CTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_001135943.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129415.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 9, mRNA.

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CloneID	OHu32000
Clone ID Related Accession (Same CDS sequence)	NM_001135943.1
Accession Version	NM_001135943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA759613.1, BC040484.1, AF440100.1 and U77352.1. Transcript Variant: This variant (9) differs in the 5' UTR, and uses two alternate in-frame splice sites and lacks three alternate in-frame exons in the mid coding region, compared to variant 4. The resulting isoform (i) is shorter than isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040484.1, SRR1803613.77631.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GCTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129415.1
CDS	216..4850
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_001135943.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135943.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGAGT 
AATAGCTCTG GAGAGACCCT TGGAGCTGAC AGTGACTTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGCG AGGGCAGTGT TCACCTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ACTCTGCCAC AAGCACCATC TTTGGTAAAG CCCACAGCTT GAAGCCAAGC 
ATAAAGGAGA AGCTGGCAGG CAGCCCCATT CGTACTTCTG AAGATGTGAG CCAGCGAGTC 
TATCTCTATG AGGGACTCCT AGGCAAAGAG CGTTCTACTT TATGGGACCA AATGCAATTC 
TGGGAAGATG CCTTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTTGGAG AACATGACCG GAAGCGCCTG 
GAAGATGATG AAGATCGCTT GCTGGCCACA CTTCTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGC AAGAAGGTGA GGCGCCTAAT GGGAAAGTCG 
CACATTGGGC TTGTGTACAG CCAGCAAATC AATGAGGTGC TTGATCAGCT GGCGAACCTG 
AATGGACGCG ATCTCTCTAT CTGGTCCAGT GGCAGCCGGC ACATGAAGAA GCAGACATTT 
GTGGTACATG CAGGGACAGA TACAAACGGA GATATCTTTT TCATGGAGGT GTGCGATGAC 
TGTGTGGTGT TGCGTAGTAA CATCGGAACA GTGTATGAGC GCTGGTGGTA CGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACGAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACCCAGC TCAACAAGTT CTATACTAAA AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGCGCTGC CGCCCGACAG CAAAGCATCA AACCCGGACC TGAATTGGGT 
GGCGAGTTCC CTGTGCAGGA CCTGAAGACT GGTGAGGGTG GCCTGCTGCA GGTGACCCTG 
GAAGGGATCA ACCTCAAATT CATGCACAAT CAGGTTTTCA TAGAGCTGAA TCACATTAAA 
AAGTGCAATA CAGTTCGAGG CGTCTTTGTC CTGGAGGAAT TTGTTCCTGA AATTAAAGAA 
GTGGTGAGCC ACAAGTACAA GACACCAATG GCCCACGAAA TCTGCTACTC CGTATTATGT 
CTCTTCTCGT ACGTGGCTGC AGTTCATAGC AGTGAGGAAG ATCTCAGAAC CCCGCCCCGG 
CCTGTCTCTA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_003682.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003673.3 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 4, mRNA.

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CloneID	OHu45057
Clone ID Related Accession (Same CDS sequence)	NM_003682.4
Accession Version	NM_003682.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Jun 2, 2019 this sequence version replaced NM_003682.3. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (d). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF440100.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003673.3
CDS	177..5120
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_003682.4
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003682.4

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GAATTGTGGA ACAAGCACCA GGAAGTGAAA AAGCAAAAAG CTTTGGAAAA ACAGAGGCCT 
GAAGTAATCA AACCTGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACGTCTAGT TCCCAGAGGA CTGATCAAGA CTCTGTCATC 
GGCGTGAGTC CAGCTGTTAT GATCCGCAGC TCAAGTCAGG ATTCTGAAGT TAGCACCGTG 
GTGAGTAATA GCTCTGGAGA GACCCTTGGA GCTGACAGTG ACTTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGCGAGGG CAGTGTTCAC CTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAACTC TGCCACAAGC ACCATCTTTG GTAAAGCCCA CAGCTTGAAG 
CCAAGCATAA AGGAGAAGCT GGCAGGCAGC CCCATTCGTA CTTCTGAAGA TGTGAGCCAG 
CGAGTCTATC TCTATGAGGG ACTCCTAGGA AGGGACAAAG GATCCATGTG GGACCAGTTA 
GAGGATGCAG CTATGGAGAC CTTTTCTATA AGCAAAGAGC GTTCTACTTT ATGGGACCAA 
ATGCAATTCT GGGAAGATGC CTTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTTGGAGA ACATGACCGG 
AAGCGCCTGG AAGATGATGA AGATCGCTTG TTGGCCACAC TTCTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGCA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCGC ACATTGGGCT TGTGTACAGC CAGCAAATCA ATGAGGTGCT TGATCAGCTG 
GCGAACCTGA ATGGACGCGA TCTCTCTATC TGGTCCAGTG GCAGCCGGCA CATGAAGAAG 
CAGACATTTG TGGTACATGC AGGGACAGAT ACAAACGGAG ATATCTTTTT CATGGAGGTG 
TGCGATGACT GTGTGGTGTT GCGTAGTAAC ATCGGAACAG TGTATGAGCG CTGGTGGTAC 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACGAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACCCAGCT CAACAAGTTC TATACTAAAA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGCGCTGCC GCCCGACAGC AAAGCATCAA ACCCGGACCT 
GAATTGGGTG GCGAGTTCCC TGTGCAGGAC CTGAAGACTG GTGAGGGTGG CCTGCTGCAG 
GTGACCCTGG AAGGGATCAA CCTCAAATTC ATGCACAATC AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTTCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCACGAAAT CTGCTACTCC 
GTATTATGTC TCTTCTCGTA CGTGGCTGCA GTTCATAGCA GTGAGGAAGA TCTCAGAACC 
CCGCCCCGGC CTGTCTCTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_130473.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_569829.2 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 5, mRNA.

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CloneID	OHu45066
Clone ID Related Accession (Same CDS sequence)	XM_005253196.3 , NM_130473.3 , XM_005253196.2
Accession Version	NM_130473.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Jun 2, 2019 this sequence version replaced NM_130473.2. Transcript Variant: This variant (5) lacks exons 21 and 26 as compared to transcript variant 4. The resulting isoform (e) is missing regions of 18 aa and 21 aa but has identical N- and C-termini as those of isoform (d) encoded by transcript variant 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF440101.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569829.2
CDS	177..5003
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_130473.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130473.3

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_130470.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_569826.2 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu45070
Clone ID Related Accession (Same CDS sequence)	XM_005253199.3 , XM_005253199.2 , NM_130470.3
Accession Version	NM_130470.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Jun 2, 2019 this sequence version replaced NM_130470.2. Transcript Variant: This variant (1) has a different exon 1 sequence and lacks exons 16, 21 and 26, as compared to transcript variant 4. The resulting isoform (a) is shorter than isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U77352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569826.2
CDS	359..5125
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_130470.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130470.3

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_130471.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_569827.2 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu45072
Clone ID Related Accession (Same CDS sequence)	XM_005253201.2 , NM_130471.3
Accession Version	NM_130471.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Jun 2, 2019 this sequence version replaced NM_130471.2. Transcript Variant: This variant (2) lacks exons 21, 26, and a portion of exon 13 as compared to transcript variant 4. The resulting isoform (b) is missing regions of 43 aa, 18 aa, and 21 aa but has identical N- and C-termini as those of isoform (d) encoded by transcript variant 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF440102.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569827.2
CDS	177..4874
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	NM_130471.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130471.3

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( NM_130474.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_569830.2 Homo sapiens MAP kinase activating death domain (MADD), transcript variant 6, mRNA.

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CloneID	OHu45077
Clone ID Related Accession (Same CDS sequence)	NM_130474.3 , XM_005253205.1 , XM_005253205.2
Accession Version	NM_130474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018410.24 and AC090582.9. On Jun 2, 2019 this sequence version replaced NM_130474.2. Transcript Variant: This variant (6) lacks exons 16, 21, 26, 34, and part of exon 13 but maintains the same reading frame up through exon 33 as compared to transcript variant 4. The resulting isoform (f) is missing regions of 43 aa, 20 aa, 18 aa, and 21 aa and has a different carboxyl terminus as compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF440434.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569830.2
CDS	177..4616
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	NM_130474.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130474.3

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253196.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253253.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OHu45066
Clone ID Related Accession (Same CDS sequence)	XM_005253196.3 , NM_130473.3 , XM_005253196.2
Accession Version	XM_005253196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253196.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253253.1
CDS	216..5042
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005253196.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253196.3

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253196.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253253.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

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CloneID	OHu45066
Clone ID Related Accession (Same CDS sequence)	XM_005253196.3 , NM_130473.3 , XM_005253196.2
Accession Version	XM_005253196.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253253.1
CDS	216..5042
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005253196.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253196.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGTATTTGG GCTAAATACT CTAATGGAGA TTGTTACTGA AGCCGGCCCC 
GGGAGTGGTG AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253199.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253256.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu45070
Clone ID Related Accession (Same CDS sequence)	XM_005253199.3 , XM_005253199.2 , NM_130470.3
Accession Version	XM_005253199.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253199.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253256.1
CDS	194..4960
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005253199.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253199.3

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253199.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253256.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu45070
Clone ID Related Accession (Same CDS sequence)	XM_005253199.3 , XM_005253199.2 , NM_130470.3
Accession Version	XM_005253199.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253256.1
CDS	216..4982
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGNRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005253199.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253199.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGTCAGTGC GCCGGCGAAT CTATGACAAT CCATACTTCG AGCCCCAATA TGGCTTTCCC 
CCTGAGGAAG ATGAGGATGA GCAGGGGGAA AGTTACACTC CCCGATTCAG CCAACATGTC 
AGTGGCAATC GGGCTCAAAA GCTGCTGCGG CCCAACAGCT TGAGACTGGC AAGTGACTCA 
GATGCAGAGT CAGACTCTCG GGCAAGCTCT CCCAACTCCA CCGTCTCCAA CACCAGCACC 
GAGGGCTTCG GGGGCATCAT GTCTTTTGCC AGCAGCCTCT ATCGGAACCA CAGTACCAGC 
TTCAGTCTTT CAAACCTCAC ACTGCCCACC AAAGGTGCCC GAGAGAAGGC CACGCCCTTC 
CCCAGTCTGA AAGGAAACAG GAGGGCGTTA GTGGATCAGA AGTCATCTGT CATTAAACAC 
AGCCCAACAG TGAAAAGAGA ACCTCCATCA CCCCAGGGTC GATCCAGCAA TTCTAGTGAG 
AACCAGCAGT TCCTGAAGGA GGTGGTGCAC AGCGTGCTGG ACGGCCAGGG AGTTGGCTGG 
CTCAACATGA AAAAGGTGCG CCGGCTGCTG GAGAGCGAGC AGCTGCGAGT CTTTGTCCTG 
AGCAAGCTGA ACCGCATGGT GCAGTCAGAG GACGATGCCC GGCAGGACAT CATCCCGGAT 
GTGGAGATCA GTCGGAAGGT GTACAAGGGA ATGTTAGACC TCCTCAAGTG TACAGTCCTC 
AGCTTGGAGC AGTCCTATGC CCACGCGGGT CTGGGTGGCA TGGCCAGCAT CTTTGGGCTT 
TTGGAGATTG CCCAGACCCA CTACTATAGT AAAGAACCAG ACAAGCGGAA GAGAAGTCCA 
ACAGAAAGTG TAAATACCCC AGTTGGCAAG GATCCTGGCC TAGCTGGGCG GGGGGACCCA 
AAGGCTATGG CACAACTGAG AGTTCCACAA CTGGGACCTC GGGCACCAAG TGCCACAGGA 
AAGGGTCCTA AGGAACTGGA CACCAGAAGT TTAAAGGAAG AAAATTTTAT AGCATCTATT 
GGGCCTGAAG TAATCAAACC TGTCTTTGAC CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG 
ATCAGCGCAG ACAGTGGTGT GAGCCTGACG TCTAGTTCCC AGAGGACTGA TCAAGACTCT 
GTCATCGGCG TGAGTCCAGC TGTTATGATC CGCAGCTCAA GTCAGGATTC TGAAGTTAGC 
ACCGTGGTGA GTAATAGCTC TGGAGAGACC CTTGGAGCTG ACAGTGACTT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG CGAGGGCAGT GTTCACCTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAACTCTGCC ACAAGCACCA TCTTTGGTAA AGCCCACAGC 
TTGAAGCCAA GCATAAAGGA GAAGCTGGCA GGCAGCCCCA TTCGTACTTC TGAAGATGTG 
AGCCAGCGAG TCTATCTCTA TGAGGGACTC CTAGGCAAAG AGCGTTCTAC TTTATGGGAC 
CAAATGCAAT TCTGGGAAGA TGCCTTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTTGG AGAACATGAC 
CGGAAGCGCC TGGAAGATGA TGAAGATCGC TTGTTGGCCA CACTTCTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GCAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CGCACATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAGGT GCTTGATCAG 
CTGGCGAACC TGAATGGACG CGATCTCTCT ATCTGGTCCA GTGGCAGCCG GCACATGAAG 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GATACAAACG GAGATATCTT TTTCATGGAG 
GTGTGCGATG ACTGTGTGGT GTTGCGTAGT AACATCGGAA CAGTGTATGA GCGCTGGTGG 
TACGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACGA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACCCA GCTCAACAAG TTCTATACTA AAAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGCGCT GCCGCCCGAC AGCAAAGCAT CAAACCCGGA 
CCTGAATTGG GTGGCGAGTT CCCTGTGCAG GACCTGAAGA CTGGTGAGGG TGGCCTGCTG 
CAGGTGACCC TGGAAGGGAT CAACCTCAAA TTCATGCACA ATCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTTCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCACGA AATCTGCTAC 
TCCGTATTAT GTCTCTTCTC GTACGTGGCT GCAGTTCATA GCAGTGAGGA AGATCTCAGA 
ACCCCGCCCC GGCCTGTCTC TAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253201.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253258.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

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CloneID	OHu45072
Clone ID Related Accession (Same CDS sequence)	XM_005253201.2 , NM_130471.3
Accession Version	XM_005253201.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253258.1
CDS	216..4913
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005253201.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253201.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGTATTTG GGCTAAATAC TCTAATGGAG ATTGTTACTG AAGCCGGCCC CGGGAGTGGT 
GAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253203.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253260.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

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CloneID	OHu45075
Clone ID Related Accession (Same CDS sequence)	NM_001376616.1 , XM_005253203.3
Accession Version	XM_005253203.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253203.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253260.1
CDS	198..4835
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005253203.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253203.3

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTTCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCACG AAATCTGCTA CTCCGTATTA 
TGTCTCTTCT CGTACGTGGC TGCAGTTCAT AGCAGTGAGG AAGATCTCAG AACCCCGCCC 
CGGCCTGTCT CTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253204.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253261.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X31, mRNA.

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CloneID	OHu45076
Clone ID Related Accession (Same CDS sequence)	NM_001376621.1 , NM_001376619.1 , XM_005253204.3 , XM_005253204.2
Accession Version	XM_005253204.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253204.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253261.1
CDS	191..4816
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005253204.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253204.3

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253204.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253261.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OHu45076
Clone ID Related Accession (Same CDS sequence)	NM_001376621.1 , NM_001376619.1 , XM_005253204.3 , XM_005253204.2
Accession Version	XM_005253204.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253261.1
CDS	216..4841
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005253204.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253204.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTGAG TAATAGCTCT 
GGAGAGACCC TTGGAGCTGA CAGTGACTTG AGCAGCAATG CAGGTGATGG ACCAGGTGGC 
GAGGGCAGTG TTCACCTGGC AAGCTCTCGG GGCACTTTGT CTGATAGTGA AATTGAGACC 
AACTCTGCCA CAAGCACCAT CTTTGGTAAA GCCCACAGCT TGAAGCCAAG CATAAAGGAG 
AAGCTGGCAG GCAGCCCCAT TCGTACTTCT GAAGATGTGA GCCAGCGAGT CTATCTCTAT 
GAGGGACTCC TAGGCAAAGA GCGTTCTACT TTATGGGACC AAATGCAATT CTGGGAAGAT 
GCCTTCTTAG ATGCTGTGAT GTTGGAGAGA GAAGGGATGG GTATGGACCA GGGTCCCCAG 
GAAATGATCG ACAGGTACCT GTCCCTTGGA GAACATGACC GGAAGCGCCT GGAAGATGAT 
GAAGATCGCT TGTTGGCCAC ACTTCTGCAC AACCTCATCT CCTACATGCT GCTGATGAAG 
GTAAATAAGA ATGACATCCG CAAGAAGGTG AGGCGCCTAA TGGGAAAGTC GCACATTGGG 
CTTGTGTACA GCCAGCAAAT CAATGAGGTG CTTGATCAGC TGGCGAACCT GAATGGACGC 
GATCTCTCTA TCTGGTCCAG TGGCAGCCGG CACATGAAGA AGCAGACATT TGTGGTACAT 
GCAGGGACAG ATACAAACGG AGATATCTTT TTCATGGAGG TGTGCGATGA CTGTGTGGTG 
TTGCGTAGTA ACATCGGAAC AGTGTATGAG CGCTGGTGGT ACGAGAAGCT CATCAACATG 
ACCTACTGTC CCAAGACGAA GGTGTTGTGC TTGTGGCGTA GAAATGGCTC TGAGACCCAG 
CTCAACAAGT TCTATACTAA AAAGTGTCGG GAGCTGTACT ACTGTGTGAA GGACAGCATG 
GAGCGCGCTG CCGCCCGACA GCAAAGCATC AAACCCGGAC CTGAATTGGG TGGCGAGTTC 
CCTGTGCAGG ACCTGAAGAC TGGTGAGGGT GGCCTGCTGC AGGTGACCCT GGAAGGGATC 
AACCTCAAAT TCATGCACAA TCAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT 
ACAGTTCGAG GCGTCTTTGT CCTGGAGGAA TTTGTTCCTG AAATTAAAGA AGTGGTGAGC 
CACAAGTACA AGACACCAAT GGCCCACGAA ATCTGCTACT CCGTATTATG TCTCTTCTCG 
TACGTGGCTG CAGTTCATAG CAGTGAGGAA GATCTCAGAA CCCCGCCCCG GCCTGTCTCT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253205.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253262.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X41, mRNA.

Price & Availability↑

CloneID	OHu45077
Clone ID Related Accession (Same CDS sequence)	NM_130474.3 , XM_005253205.1 , XM_005253205.2
Accession Version	XM_005253205.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253262.1
CDS	216..4655
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_005253205.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253205.1

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005253205.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253262.1 Homo sapiens MAP kinase activating death domain (MADD), transcript variant X41, mRNA.

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CloneID	OHu45077
Clone ID Related Accession (Same CDS sequence)	NM_130474.3 , XM_005253205.1 , XM_005253205.2
Accession Version	XM_005253205.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	MAP kinase-activating death domain protein isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253262.1
CDS	216..4655
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRSSSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_005253205.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAP kinase activating death domain (MADD), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253205.2

ORF Insert Sequence:

ATGGTGCAAA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTAGTGAT CGTAGGGGCC 
AGGCACCCGA GCAGTGATAG CGTGGCCCAG ACTCCTGAAT TGCTACGGCG ATACCCCTTG 
GAGGATCACA CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA GCCCGAGGGC 
TGCCTGAGCG TGCGGCAGCG GCGCATGAGC CTTCGGGATG ATACCTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACGCGA TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAAAAGC GAATCTCTAA GGAGAAGGGG GAAGGTGGGG CAGGGTCCCG TGGGAAGGAA 
GGAACCCATG CCACCTGTGC CTCAGAAGAG GGTGGCACTG AGAGCTCAGA GAGTGGCTCA 
TCCCTGCAGC CTCTCAGTGC TGACTCTACC CCTGATGTGA ACCAGTCTCC TCGGGGCAAA 
CGCCGGGCCA AGGCGGGGAG CCGCTCCCGC AACAGTACTC TCACGTCCCT GTGCGTGCTC 
AGCCACTACC CTTTCTTCTC CACCTTCCGA GAGTGTTTGT ATACTCTCAA GCGCCTGGTG 
GACTGCTGTA GTGAGCGCCT TCTGGGCAAG AAACTGGGCA TCCCTCGAGG CGTACAAAGG 
GACACCATGT GGCGGATCTT TACTGGATCG CTGCTGGTAG AGGAGAAGTC AAGTGCCCTT 
CTGCATGACC TTCGAGAGAT TGAGGCCTGG ATCTATCGAT TGCTGCGCTC CCCAGTACCC 
GTCTCTGGGC AGAAGCGAGT AGACATCGAG GTCCTACCCC AAGAGCTCCA GCCAGCTCTG 
ACCTTTGCTC TTCCAGACCC ATCTCGATTC ACCCTAGTGG ATTTCCCACT GCACCTTCCC 
TTGGAACTTC TAGGTGTGGA CGCCTGTCTC CAGGTGCTAA CCTGCATTCT GTTAGAGCAC 
AAGGTGGTGC TACAGTCCCG AGACTACAAT GCACTCTCCA TGTCTGTGAT GGCATTCGTG 
GCAATGATCT ACCCACTGGA GTATATGTTT CCTGTCATCC CGCTGCTACC CACCTGCATG 
GCATCAGCAG AGCAGCTGCT GTTGGCTCCA ACCCCGTACA TCATTGGGGT TCCTGCCAGC 
TTCTTCCTCT ACAAACTGGA CTTCAAAATG CCTGATGATG TATGGCTAGT GGATCTGGAC 
AGCAATAGGG TGATTGCCCC CACCAATGCA GAAGTGCTGC CTATCCTGCC AGAACCAGAA 
TCACTAGAGC TGAAAAAGCA TTTAAAGCAG GCCTTGGCCA GCATGAGTCT CAACACCCAG 
CCCATCCTCA ATCTGGAGAA ATTTCATGAG GGCCAGGAGA TCCCCCTTCT CTTGGGAAGG 
CCTTCTAATG ACCTGCAGTC CACACCGTCC ACTGAATTCA ACCCACTCAT CTATGGCAAT 
GATGTGGATT CTGTGGATGT TGCAACCAGG GTTGCCATGG TACGGTTCTT CAATTCCGCC 
AACGTGCTGC AGGGATTTCA GATGCACACG CGTACCCTGC GCCTCTTTCC TCGGCCTGTG 
GTAGCTTTTC AAGCTGGCTC CTTTCTAGCC TCACGTCCCC GGCAGACTCC TTTTGCCGAG 
AAATTGGCCA GGACTCAGGC TGTGGAGTAC TTTGGGGAAT GGATCCTTAA CCCCACCAAC 
TATGCCTTTC AGCGAATTCA CAACAATATG TTTGATCCAG CCCTGATTGG TGACAAGCCA 
AAGTGGTATG CTCATCAGCT GCAGCCTATC CACTATCGCG TCTATGACAG CAATTCCCAG 
CTGGCTGAGG CCCTGAGTGT ACCACCAGAG CGGGACTCTG ACTCCGAACC TACTGATGAT 
AGTGGCAGTG ATAGTATGGA TTATGACGAT TCAAGCTCTT CTTACTCCTC CCTTGGTGAC 
TTTGTCAGTG AAATGATGAA ATGTGACATT AATGGTGATA CTCCCAATGT GGACCCTCTG 
ACACATGCAG CACTGGGGGA TGCCAGCGAG GTGGAGATTG ACGAGCTGCA GAATCAGAAG 
GAAGCAGAAG AGCCTGGCCC AGACAGTGAG AACTCTCAGG AAAACCCCCC ACTGCGCTCC 
AGCTCTAGCA CCACAGCCAG CAGCAGCCCC AGCACTGTCA TCCACGGAGC CAACTCTGAA 
CCTGCTGACT CTACGGAGAT GGATGATAAG GCAGCAGTAG GCGTCTCCAA GCCCCTCCCT 
TCCGTGCCTC CCAGCATTGG CAAATCGAAC GTGGACAGAC GTCAGGCAGA AATTGGAGAG 
GGGGCTCAAA AGCTGCTGCG GCCCAACAGC TTGAGACTGG CAAGTGACTC AGATGCAGAG 
TCAGACTCTC GGGCAAGCTC TCCCAACTCC ACCGTCTCCA ACACCAGCAC CGAGGGCTTC 
GGGGGCATCA TGTCTTTTGC CAGCAGCCTC TATCGGAACC ACAGTACCAG CTTCAGTCTT 
TCAAACCTCA CACTGCCCAC CAAAGGTGCC CGAGAGAAGG CCACGCCCTT CCCCAGTCTG 
AAAGGAAACA GGAGGGCGTT AGTGGATCAG AAGTCATCTG TCATTAAACA CAGCCCAACA 
GTGAAAAGAG AACCTCCATC ACCCCAGGGT CGATCCAGCA ATTCTAGTGA GAACCAGCAG 
TTCCTGAAGG AGGTGGTGCA CAGCGTGCTG GACGGCCAGG GAGTTGGCTG GCTCAACATG 
AAAAAGGTGC GCCGGCTGCT GGAGAGCGAG CAGCTGCGAG TCTTTGTCCT GAGCAAGCTG 
AACCGCATGG TGCAGTCAGA GGACGATGCC CGGCAGGACA TCATCCCGGA TGTGGAGATC 
AGTCGGAAGG TGTACAAGGG AATGTTAGAC CTCCTCAAGT GTACAGTCCT CAGCTTGGAG 
CAGTCCTATG CCCACGCGGG TCTGGGTGGC ATGGCCAGCA TCTTTGGGCT TTTGGAGATT 
GCCCAGACCC ACTACTATAG TAAAGAACCA GACAAGCGGA AGAGAAGTCC AACAGAAAGT 
GTAAATACCC CAGTTGGCAA GGATCCTGGC CTAGCTGGGC GGGGGGACCC AAAGGCTATG 
GCACAACTGA GAGTTCCACA ACTGGGACCT CGGGCACCAA GTGCCACAGG AAAGGGTCCT 
AAGGAACTGG ACACCAGAAG TTTAAAGGAA GAAAATTTTA TAGCATCTAT TGGGCCTGAA 
GTAATCAAAC CTGTCTTTGA CCTTGGTGAG ACAGAGGAGA AAAAGTCCCA GATCAGCGCA 
GACAGTGGTG TGAGCCTGAC GTCTAGTTCC CAGAGGACTG ATCAAGACTC TGTCATCGGC 
GTGAGTCCAG CTGTTATGAT CCGCAGCTCA AGTCAGGATT CTGAAGTTAG CACCGTGGTG 
AGTAATAGCT CTGGAGAGAC CCTTGGAGCT GACAGTGACT TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GCGAGGGCAG TGTTCACCTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAACTCTGC CACAAGCACC ATCTTTGGTA AAGCCCACAG CTTGAAGCCA 
AGCATAAAGG AGAAGCTGGC AGGCAGCCCC ATTCGTACTT CTGAAGATGT GAGCCAGCGA 
GTCTATCTCT ATGAGGGACT CCTAGGCAAA GAGCGTTCTA CTTTATGGGA CCAAATGCAA 
TTCTGGGAAG ATGCCTTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTTG GAGAACATGA CCGGAAGCGC 
CTGGAAGATG ATGAAGATCG CTTGTTGGCC ACACTTCTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGCAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCGCACATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAGG TGCTTGATCA GCTGGCGAAC 
CTGAATGGAC GCGATCTCTC TATCTGGTCC AGTGGCAGCC GGCACATGAA GAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGATACAAAC GGAGATATCT TTTTCATGGA GGTGTGCGAT 
GACTGTGTGG TGTTGCGTAG TAACATCGGA ACAGTGTATG AGCGCTGGTG GTACGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACG AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACCC AGCTCAACAA GTTCTATACT AAAAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGCGC TGCCGCCCGA CAGCAAAGCA TCAAACCCGG ACCTGAATTG 
GGTGGCGAGT TCCCTGTGCA GGACCTGAAG ACTGGTGAGG GTGGCCTGCT GCAGGTGACC 
CTGGAAGGGA TCAACCTCAA ATTCATGCAC AATCAGTTCC TGAAATTAAA GAAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MADD-FOLH1 Polymorphisms and Their Haplotypes with Serum Lipid Levels and the Risk of Coronary Heart Disease and Ischemic Stroke in a Chinese Han Population.
Nutrients8(4)208(2016 Apr)
Wu DF,Yin RX,Cao XL,Huang F,Wu JZ,Chen WX

Epigenetic silencing of tumor suppressor miR-3151 contributes to Chinese chronic lymphocytic leukemia by constitutive activation of MADD/ERK and PIK3R2/AKT signaling pathways.
Oncotarget6(42)44422-36(2015 Dec)
Wang LQ,Wong KY,Ros?n A,Chim CS

MADD is a downstream target of PTEN in triggering apoptosis.
Journal of cellular biochemistry115(2)261-70(2014 Feb)
Jayarama S,Li LC,Ganesh L,Mardi D,Kanteti P,Hay N,Li P,Prabhakar BS

MADD promotes the survival of human lung adenocarcinoma cells by inhibiting apoptosis.
Oncology reports29(4)1533-9(2013 Apr)
Bi W,Wei Y,Wu J,Sun G,Guo Y,Zhang Q,Dong L

MADD knock-down enhances doxorubicin and TRAIL induced apoptosis in breast cancer cells.
PloS one8(2)e56817(2013)
Turner A,Li LC,Pilli T,Qian L,Wiley EL,Setty S,Christov K,Ganesh L,Maker AV,Li P,Kanteti P,Das Gupta TK,Prabhakar BS

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MADD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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