DENR cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DENR
Entrez Gene ID	8562
Full Name	density regulated re-initiation and release factor
Synonyms	DRP,DRP1,SMAP-3
General protein information	Preferred Names density regulated re-initiation and release factor Names density-regulated protein smooth muscle cell associated protein-3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Summary	This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants. [provided by RefSeq, Jul 2008].
Disorder MIM:	604550

mRNA and Protein(s)

mRNA	Protein	Name
NM_003677.5	NP_003668.2	density-regulated protein
NM_003677.4	NP_003668.2	density-regulated protein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DENR	NP_001072973.1
Homo sapiens (human)	DENR	NP_003668.2
Caenorhabditis elegans (roundworm)	Y47D3A.21	NP_499450.1
Saccharomyces cerevisiae (baker's yeast)	TMA22	NP_012548.1
Magnaporthe oryzae (rice blast fungus)	MGG_04504	XP_003710951.1
Pan troglodytes (chimpanzee)	DENR	XP_003952390.1
Mus musculus (house mouse)	Denr	NP_080879.1
Kluyveromyces lactis	KLLA0F21934g	XP_456059.1
Neurospora crassa	NCU02984	XP_965370.1
Arabidopsis thaliana (thale cress)	AT5G11900	NP_196751.2
Rattus norvegicus (Norway rat)	LOC687565	NP_001108518.1
Drosophila melanogaster (fruit fly)	CG9099	NP_001259630.1
Schizosaccharomyces pombe (fission yeast)	SPBC16C6.05	NP_596803.1
Oryza sativa (rice)	Os01g0195500	NP_001042291.2
Xenopus tropicalis (tropical clawed frog)	denr	NP_001006814.1
Canis lupus familiaris (dog)	DENR	XP_853345.3
Bos taurus (cattle)	DENR	NP_001039993.1
Danio rerio (zebrafish)	denr	NP_001002697.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006125	XP_316185.3
Eremothecium gossypii	AGOS_ACL112C	NP_983292.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural and Functional Insights into Human Re-initiation Complexes.
Weisser M, Sch?fer T, Leibundgut M, B?hringer D, Aylett CHS, Ban N
Molecular cell67(3)447-456.e7(2017 Aug)

Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1.
Lomakin IB, Stolboushkina EA, Vaidya AT, Zhao C, Garber MB, Dmitriev SE, Steitz TA
Cell reports20(3)521-528(2017 Jul)

De Novo Mutations in DENR Disrupt Neuronal Development and Link Congenital Neurological Disorders to Faulty mRNA Translation Re-initiation.
Haas MA, Ngo L, Li SS, Schleich S, Qu Z, Vanyai HK, Cullen HD, Cardona-Alberich A, Gladwyn-Ng IE, Pagnamenta AT, Taylor JC, Stewart H, Kini U, Duncan KE, Teleman AA, Keays DA, Heng JI
Cell reports15(10)2251-2265(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Reinitiation complexes involving initiation factors eIF2D, MCT-1, and DENR controls the translation of a large fraction of mammalian cellular mRNAs.
Title: Structural and Functional Insights into Human Re-initiation Complexes.

DENR binds to the P-site of the 40S ribosomal subunit and together with MCTS1 forms a tRNA binding surface and interfere with eIF1/eIF2/eIF3 binding, thus operating in ribosome recycling and translation re-initiation
Title: Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1.

both HuR and AUF1 bind to discrete regions of DENR/DRP mRNA and that AUF1 silencing increases DENR/DRP protein levels.
Title: Post-transcriptional control of the MCT-1-associated protein DENR/DRP by RNA-binding protein AUF1.

The following DENR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03472	NM_003677.5 Latest version!	Homo sapiens density regulated re-initiation and release factor (DENR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu03472	NM_003677.4	Homo sapiens density regulated re-initiation and release factor (DENR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DENR ( NM_003677.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003668.2 Homo sapiens density regulated re-initiation and release factor (DENR), mRNA.

Price & Availability↑

CloneID	OHu03472
Clone ID Related Accession (Same CDS sequence)	NM_003677.5 , NM_003677.4
Accession Version	NM_003677.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	density-regulated protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP872988.1, AB014731.1, CB043904.1, AF103800.1, CN300034.1 and AI093455.1. On Nov 23, 2018 this sequence version replaced NM_003677.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.261324.1, SRR1660807.117039.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000280557.11/ ENSP00000280557.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCTG ACATTTCTGA ATCCAGCGGG GCTGACTGCA AAGGAGACCC AAGGAACAGT 
GCCAAGTTAG ATGCCGATTA CCCACTTCGA GTCCTTTATT GTGGAGTCTG TTCATTACCA 
ACAGAGTACT GTGAATATAT GCCTGATGTT GCTAAATGTA GACAATGGTT AGAGAAGAAT 
TTTCCAAATG AATTTGCAAA ACTTACTGTA GAAAATTCAC CCAAACAAGA AGCTGGAATT 
AGTGAGGGTC AAGGAACAGC AGGGGAAGAA GAGGAGAAGA AAAAACAGAA GAGAGGTGGA 
AGGGGTCAAA TAAAACAAAA AAAGAAGACC GTACCACAAA AGGTTACTAT AGCCAAAATT 
CCCAGAGCAA AGAAGAAATA TGTGACAAGA GTATGTGGCC TTGCAACTTT TGAAATTGAT 
CTTAAAGAAG CACAAAGATT TTTTGCTCAA AAATTCTCCT GTGGTGCCTC AGTAACAGGG 
GAGGATGAAA TTATCATTCA GGGAGATTTT ACAGATGACA TAATTGATGT CATTCAGGAA 
AAATGGCCAG AGGTAGATGA TGACAGCATC GAAGATCTTG GAGAAGTAAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003668.2
CDS	137..733
Translation	MAADISESSGADCKGDPRNSAKLDADYPLRVLYCGVCSLPTEYCEYMPDVAKCRQWLEKNFPNEFAKLTVENSPKQEAGISEGQGTAGEEEEKKKQKRGGRGQIKQKKKTVPQKVTIAKIPRAKKKYVTRVCGLATFEIDLKEAQRFFAQKFSCGASVTGEDEIIIQGDFTDDIIDVIQEKWPEVDDDSIEDLGEVKK

Target ORF information:

RefSeq Version	NM_003677.5
Organism	Homo sapiens(human)
Definition	Homo sapiens density regulated re-initiation and release factor (DENR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003677.5

ORF Insert Sequence:

ATGGCTGCTG ACATTTCTGA ATCCAGCGGG GCTGACTGCA AAGGAGACCC AAGGAACAGT 
GCCAAGTTAG ATGCCGATTA CCCACTTCGA GTCCTTTATT GTGGAGTCTG TTCATTACCA 
ACAGAGTACT GTGAATATAT GCCTGATGTT GCTAAATGTA GACAATGGTT AGAGAAGAAT 
TTTCCAAATG AATTTGCAAA ACTTACTGTA GAAAATTCAC CCAAACAAGA AGCTGGAATT 
AGTGAGGGTC AAGGAACAGC AGGGGAAGAA GAGGAGAAGA AAAAACAGAA GAGAGGTGGA 
AGGGGTCAAA TAAAACAAAA AAAGAAGACC GTACCACAAA AGGTTACTAT AGCCAAAATT 
CCCAGAGCAA AGAAGAAATA TGTGACAAGA GTATGTGGCC TTGCAACTTT TGAAATTGAT 
CTTAAAGAAG CACAAAGATT TTTTGCTCAA AAATTCTCCT GTGGTGCCTC AGTAACAGGG 
GAGGATGAAA TTATCATTCA GGGAGATTTT ACAGATGACA TAATTGATGT CATTCAGGAA 
AAATGGCCAG AGGTAGATGA TGACAGCATC GAAGATCTTG GAGAAGTAAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENR ( NM_003677.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003668.2 Homo sapiens density regulated re-initiation and release factor (DENR), mRNA.

Price & Availability↑

CloneID	OHu03472
Clone ID Related Accession (Same CDS sequence)	NM_003677.5 , NM_003677.4
Accession Version	NM_003677.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-30
Organism	Homo sapiens(human)
Product	density-regulated protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP872988.1, AB014731.1, CB043904.1, AF103800.1, CN300034.1 and AI093455.1. On Feb 10, 2015 this sequence version replaced NM_003677.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.235372.1, SRR1660807.117039.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCTG ACATTTCTGA ATCCAGCGGG GCTGACTGCA AAGGAGACCC AAGGAACAGT 
GCCAAGTTAG ATGCCGATTA CCCACTTCGA GTCCTTTATT GTGGAGTCTG TTCATTACCA 
ACAGAGTACT GTGAATATAT GCCTGATGTT GCTAAATGTA GACAATGGTT AGAGAAGAAT 
TTTCCAAATG AATTTGCAAA ACTTACTGTA GAAAATTCAC CCAAACAAGA AGCTGGAATT 
AGTGAGGGTC AAGGAACAGC AGGGGAAGAA GAGGAGAAGA AAAAACAGAA GAGAGGTGGA 
AGGGGTCAAA TAAAACAAAA AAAGAAGACC GTACCACAAA AGGTTACTAT AGCCAAAATT 
CCCAGAGCAA AGAAGAAATA TGTGACAAGA GTATGTGGCC TTGCAACTTT TGAAATTGAT 
CTTAAAGAAG CACAAAGATT TTTTGCTCAA AAATTCTCCT GTGGTGCCTC AGTAACAGGG 
GAGGATGAAA TTATCATTCA GGGAGATTTT ACAGATGACA TAATTGATGT CATTCAGGAA 
AAATGGCCAG AGGTAGATGA TGACAGCATC GAAGATCTTG GAGAAGTAAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003668.2
CDS	140..736
Translation	MAADISESSGADCKGDPRNSAKLDADYPLRVLYCGVCSLPTEYCEYMPDVAKCRQWLEKNFPNEFAKLTVENSPKQEAGISEGQGTAGEEEEKKKQKRGGRGQIKQKKKTVPQKVTIAKIPRAKKKYVTRVCGLATFEIDLKEAQRFFAQKFSCGASVTGEDEIIIQGDFTDDIIDVIQEKWPEVDDDSIEDLGEVKK

Target ORF information:

RefSeq Version	NM_003677.4
Organism	Homo sapiens(human)
Definition	Homo sapiens density regulated re-initiation and release factor (DENR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003677.4

ORF Insert Sequence:

ATGGCTGCTG ACATTTCTGA ATCCAGCGGG GCTGACTGCA AAGGAGACCC AAGGAACAGT 
GCCAAGTTAG ATGCCGATTA CCCACTTCGA GTCCTTTATT GTGGAGTCTG TTCATTACCA 
ACAGAGTACT GTGAATATAT GCCTGATGTT GCTAAATGTA GACAATGGTT AGAGAAGAAT 
TTTCCAAATG AATTTGCAAA ACTTACTGTA GAAAATTCAC CCAAACAAGA AGCTGGAATT 
AGTGAGGGTC AAGGAACAGC AGGGGAAGAA GAGGAGAAGA AAAAACAGAA GAGAGGTGGA 
AGGGGTCAAA TAAAACAAAA AAAGAAGACC GTACCACAAA AGGTTACTAT AGCCAAAATT 
CCCAGAGCAA AGAAGAAATA TGTGACAAGA GTATGTGGCC TTGCAACTTT TGAAATTGAT 
CTTAAAGAAG CACAAAGATT TTTTGCTCAA AAATTCTCCT GTGGTGCCTC AGTAACAGGG 
GAGGATGAAA TTATCATTCA GGGAGATTTT ACAGATGACA TAATTGATGT CATTCAGGAA 
AAATGGCCAG AGGTAGATGA TGACAGCATC GAAGATCTTG GAGAAGTAAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural and Functional Insights into Human Re-initiation Complexes.
Molecular cell67(3)447-456.e7(2017 Aug)
Weisser M,Sch?fer T,Leibundgut M,B?hringer D,Aylett CHS,Ban N

Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1.
Cell reports20(3)521-528(2017 Jul)
Lomakin IB,Stolboushkina EA,Vaidya AT,Zhao C,Garber MB,Dmitriev SE,Steitz TA

De Novo Mutations in DENR Disrupt Neuronal Development and Link Congenital Neurological Disorders to Faulty mRNA Translation Re-initiation.
Cell reports15(10)2251-2265(2016 06)
Haas MA,Ngo L,Li SS,Schleich S,Qu Z,Vanyai HK,Cullen HD,Cardona-Alberich A,Gladwyn-Ng IE,Pagnamenta AT,Taylor JC,Stewart H,Kini U,Duncan KE,Teleman AA,Keays DA,Heng JI

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DENR cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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