LGR5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LGR5
Entrez Gene ID	8549
Full Name	leucine rich repeat containing G protein-coupled receptor 5
Synonyms	FEX,GPR49,GPR67,GRP49,HG38
General protein information	Preferred Names leucine rich repeat containing G protein-coupled receptor 5 Names leucine-rich repeat-containing G-protein coupled receptor 5 G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 orphan G protein-coupled receptor HG38
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q21.1
Summary	The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015].
Disorder MIM:	606667

mRNA and Protein(s)

mRNA	Protein	Name
NM_001277226.1	NP_001264155.1	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2 precursor
NM_001277227.1	NP_001264156.1	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 3 precursor
NM_001277226.2	NP_001264155.1	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2 precursor
NM_003667.4	NP_003658.1	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 1 precursor
NM_003667.3	NP_003658.1	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 1 precursor
NM_001277227.2	NP_001264156.1	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LGR5	XP_003313909.1
	LGR5	XP_001117502.1
Drosophila melanogaster (fruit fly)	rk	NP_476702.1
Mus musculus (house mouse)	Lgr5	NP_034325.2
Homo sapiens (human)	LGR5	NP_003658.1
Gallus gallus (chicken)	LGR5	XP_425441.2
Rattus norvegicus (Norway rat)	Lgr5	NP_001100254.1
Bos taurus (cattle)	LGR5	NP_001179449.1
Anopheles gambiae (African malaria mosquito)	GPRRK	XP_317111.1
Xenopus tropicalis (tropical clawed frog)	lgr5	XP_002940219.2

Gene Ontology

The following LGR5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LGR5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20901	NM_001277227.2 Latest version!	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu21103	NM_003667.4 Latest version!	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu21443	NM_001277226.2 Latest version!	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu21443	NM_001277226.1	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu21103	NM_003667.3	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu20901	NM_001277227.1	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LGR5 ( NM_001277227.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264156.1 Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20901
Clone ID Related Accession (Same CDS sequence)	NM_001277227.1 , NM_001277227.2
Accession Version	NM_001277227.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC323352.1, DR003044.1, FN820440.1, AC078860.19 and CA412916.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FN820440.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTAC ATCTCCATAA CAATAGAATC CACTCCCTGG GAAAGAAATG CTTTGATGGG 
CTCCACAGCC TAGAGACTTT AGATTTAAAT TACAATAACC TTGATGAATT CCCCACTGCA 
ATTAGGACAC TCTCCAACCT TAAAGAACTA GGATTTCATA GCAACAATAT CAGGTCGATA 
CCTGAGAAAG CATTTGTAGG CAACCCTTCT CTTATTACAA TACATTTCTA TGACAATCCC 
ATCCAGTTTG TTGGGAGATC TGCTTTTCAA CATTTACCTG AACTAAGAAC ACTGACTCTG 
AATGGTGCCT CACAAATAAC TGAATTTCCT GATTTAACTG GAACTGCAAA CCTGGAGAGT 
CTGACTTTAA CTGGAGCACA GATCTCATCT CTTCCTCAAA CCGTCTGCAA TCAGTTACCT 
AATCTCCAAG TGCTAGATCT GTCTTACAAC CTATTAGAAG ATTTACCCAG TTTTTCAGTC 
TGCCAAAAGC TTCAGAAAAT TGACCTAAGA CATAATGAAA TCTACGAAAT TAAAGTTGAC 
ACTTTCCAGC AGTTGCTTAG CCTCCGATCG CTGAATTTGG CTTGGAACAA AATTGCTATT 
ATTCACCCCA ATGCATTTTC CACTTTGCCA TCCCTAATAA AGCTGGACCT ATCGTCCAAC 
CTCCTGTCGT CTTTTCCTAT AACTGGGTTA CATGGTTTAA CTCACTTAAA ATTAACAGGA 
AATCATGCCT TACAGAGCTT GATATCATCT GAAAACTTTC CAGAACTCAA GGTTATAGAA 
ATGCCTTATG CTTACCAGTG CTGTGCATTT GGAGTGTGTG AGAATGCCTA TAAGATTTCT 
AATCAATGGA ATAAAGGTGA CAACAGCAGT ATGGACGACC TTCATAAGAA AGATGCTGGA 
ATGTTTCAGG CTCAAGATGA ACGTGACCTT GAAGATTTCC TGCTTGACTT TGAGGAAGAC 
CTGAAAGCCC TTCATTCAGT GCAGTGTTCA CCTTCCCCAG GCCCCTTCAA ACCCTGTGAA 
CACCTGCTTG ATGGCTGGCT GATCAGAATT GGAGTGTGGA CCATAGCAGT TCTGGCACTT 
ACTTGTAATG CTTTGGTGAC TTCAACAGTT TTCAGATCCC CTCTGTACAT TTCCCCCATT 
AAACTGTTAA TTGGGGTCAT CGCAGCAGTG AACATGCTCA CGGGAGTCTC CAGTGCCGTG 
CTGGCTGGTG TGGATGCGTT CACTTTTGGC AGCTTTGCAC GACATGGTGC CTGGTGGGAG 
AATGGGGTTG GTTGCCATGT CATTGGTTTT TTGTCCATTT TTGCTTCAGA ATCATCTGTT 
TTCCTGCTTA CTCTGGCAGC CCTGGAGCGT GGGTTCTCTG TGAAATATTC TGCAAAATTT 
GAAACGAAAG CTCCATTTTC TAGCCTGAAA GTAATCATTT TGCTCTGTGC CCTGCTGGCC 
TTGACCATGG CCGCAGTTCC CCTGCTGGGT GGCAGCAAGT ATGGCGCCTC CCCTCTCTGC 
CTGCCTTTGC CTTTTGGGGA GCCCAGCACC ATGGGCTACA TGGTCGCTCT CATCTTGCTC 
AATTCCCTTT GCTTCCTCAT GATGACCATT GCCTACACCA AGCTCTACTG CAATTTGGAC 
AAGGGAGACC TGGAGAATAT TTGGGACTGC TCTATGGTAA AACACATTGC CCTGTTGCTC 
TTCACCAACT GCATCCTAAA CTGCCCTGTG GCTTTCTTGT CCTTCTCCTC TTTAATAAAC 
CTTACATTTA TCAGTCCTGA AGTAATTAAG TTTATCCTTC TGGTGGTAGT CCCACTTCCT 
GCATGTCTCA ATCCCCTTCT CTACATCTTG TTCAATCCTC ACTTTAAGGA GGATCTGGTG 
AGCCTGAGAA AGCAAACCTA CGTCTGGACA AGATCAAAAC ACCCAAGCTT GATGTCAATT 
AACTCTGATG ATGTCGAAAA ACAGTCCTGT GACTCAACTC AAGCCTTGGT AACCTTTACC 
AGCTCCAGCA TCACTTATGA CCTGCCTCCC AGTTCCGTGC CATCACCAGC TTATCCAGTG 
ACTGAGAGCT GCCATCTTTC CTCTGTGGCA TTTGTCCCAT GTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264156.1
CDS	312..2819
Translation	MDTSRLGVLLSLPVLLQLATGGSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIRIGVWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHGAWWENGVGCHVIGFLSIFASESSVFLLTLAALERGFSVKYSAKFETKAPFSSLKVIILLCALLALTMAAVPLLGGSKYGASPLCLPLPFGEPSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLENIWDCSMVKHIALLLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL

Target ORF information:

RefSeq Version	NM_001277227.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277227.1

ORF Insert Sequence:

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTAC ATCTCCATAA CAATAGAATC CACTCCCTGG GAAAGAAATG CTTTGATGGG 
CTCCACAGCC TAGAGACTTT AGATTTAAAT TACAATAACC TTGATGAATT CCCCACTGCA 
ATTAGGACAC TCTCCAACCT TAAAGAACTA GGATTTCATA GCAACAATAT CAGGTCGATA 
CCTGAGAAAG CATTTGTAGG CAACCCTTCT CTTATTACAA TACATTTCTA TGACAATCCC 
ATCCAGTTTG TTGGGAGATC TGCTTTTCAA CATTTACCTG AACTAAGAAC ACTGACTCTG 
AATGGTGCCT CACAAATAAC TGAATTTCCT GATTTAACTG GAACTGCAAA CCTGGAGAGT 
CTGACTTTAA CTGGAGCACA GATCTCATCT CTTCCTCAAA CCGTCTGCAA TCAGTTACCT 
AATCTCCAAG TGCTAGATCT GTCTTACAAC CTATTAGAAG ATTTACCCAG TTTTTCAGTC 
TGCCAAAAGC TTCAGAAAAT TGACCTAAGA CATAATGAAA TCTACGAAAT TAAAGTTGAC 
ACTTTCCAGC AGTTGCTTAG CCTCCGATCG CTGAATTTGG CTTGGAACAA AATTGCTATT 
ATTCACCCCA ATGCATTTTC CACTTTGCCA TCCCTAATAA AGCTGGACCT ATCGTCCAAC 
CTCCTGTCGT CTTTTCCTAT AACTGGGTTA CATGGTTTAA CTCACTTAAA ATTAACAGGA 
AATCATGCCT TACAGAGCTT GATATCATCT GAAAACTTTC CAGAACTCAA GGTTATAGAA 
ATGCCTTATG CTTACCAGTG CTGTGCATTT GGAGTGTGTG AGAATGCCTA TAAGATTTCT 
AATCAATGGA ATAAAGGTGA CAACAGCAGT ATGGACGACC TTCATAAGAA AGATGCTGGA 
ATGTTTCAGG CTCAAGATGA ACGTGACCTT GAAGATTTCC TGCTTGACTT TGAGGAAGAC 
CTGAAAGCCC TTCATTCAGT GCAGTGTTCA CCTTCCCCAG GCCCCTTCAA ACCCTGTGAA 
CACCTGCTTG ATGGCTGGCT GATCAGAATT GGAGTGTGGA CCATAGCAGT TCTGGCACTT 
ACTTGTAATG CTTTGGTGAC TTCAACAGTT TTCAGATCCC CTCTGTACAT TTCCCCCATT 
AAACTGTTAA TTGGGGTCAT CGCAGCAGTG AACATGCTCA CGGGAGTCTC CAGTGCCGTG 
CTGGCTGGTG TGGATGCGTT CACTTTTGGC AGCTTTGCAC GACATGGTGC CTGGTGGGAG 
AATGGGGTTG GTTGCCATGT CATTGGTTTT TTGTCCATTT TTGCTTCAGA ATCATCTGTT 
TTCCTGCTTA CTCTGGCAGC CCTGGAGCGT GGGTTCTCTG TGAAATATTC TGCAAAATTT 
GAAACGAAAG CTCCATTTTC TAGCCTGAAA GTAATCATTT TGCTCTGTGC CCTGCTGGCC 
TTGACCATGG CCGCAGTTCC CCTGCTGGGT GGCAGCAAGT ATGGCGCCTC CCCTCTCTGC 
CTGCCTTTGC CTTTTGGGGA GCCCAGCACC ATGGGCTACA TGGTCGCTCT CATCTTGCTC 
AATTCCCTTT GCTTCCTCAT GATGACCATT GCCTACACCA AGCTCTACTG CAATTTGGAC 
AAGGGAGACC TGGAGAATAT TTGGGACTGC TCTATGGTAA AACACATTGC CCTGTTGCTC 
TTCACCAACT GCATCCTAAA CTGCCCTGTG GCTTTCTTGT CCTTCTCCTC TTTAATAAAC 
CTTACATTTA TCAGTCCTGA AGTAATTAAG TTTATCCTTC TGGTGGTAGT CCCACTTCCT 
GCATGTCTCA ATCCCCTTCT CTACATCTTG TTCAATCCTC ACTTTAAGGA GGATCTGGTG 
AGCCTGAGAA AGCAAACCTA CGTCTGGACA AGATCAAAAC ACCCAAGCTT GATGTCAATT 
AACTCTGATG ATGTCGAAAA ACAGTCCTGT GACTCAACTC AAGCCTTGGT AACCTTTACC 
AGCTCCAGCA TCACTTATGA CCTGCCTCCC AGTTCCGTGC CATCACCAGC TTATCCAGTG 
ACTGAGAGCT GCCATCTTTC CTCTGTGGCA TTTGTCCCAT GTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR5 ( NM_001277227.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264156.1 Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20901
Clone ID Related Accession (Same CDS sequence)	NM_001277227.1 , NM_001277227.2
Accession Version	NM_001277227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR003044.1, FN820440.1, AC078860.19 and CA412916.1. On May 31, 2019 this sequence version replaced NM_001277227.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FN820440.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTAC ATCTCCATAA CAATAGAATC CACTCCCTGG GAAAGAAATG CTTTGATGGG 
CTCCACAGCC TAGAGACTTT AGATTTAAAT TACAATAACC TTGATGAATT CCCCACTGCA 
ATTAGGACAC TCTCCAACCT TAAAGAACTA GGATTTCATA GCAACAATAT CAGGTCGATA 
CCTGAGAAAG CATTTGTAGG CAACCCTTCT CTTATTACAA TACATTTCTA TGACAATCCC 
ATCCAGTTTG TTGGGAGATC TGCTTTTCAA CATTTACCTG AACTAAGAAC ACTGACTCTG 
AATGGTGCCT CACAAATAAC TGAATTTCCT GATTTAACTG GAACTGCAAA CCTGGAGAGT 
CTGACTTTAA CTGGAGCACA GATCTCATCT CTTCCTCAAA CCGTCTGCAA TCAGTTACCT 
AATCTCCAAG TGCTAGATCT GTCTTACAAC CTATTAGAAG ATTTACCCAG TTTTTCAGTC 
TGCCAAAAGC TTCAGAAAAT TGACCTAAGA CATAATGAAA TCTACGAAAT TAAAGTTGAC 
ACTTTCCAGC AGTTGCTTAG CCTCCGATCG CTGAATTTGG CTTGGAACAA AATTGCTATT 
ATTCACCCCA ATGCATTTTC CACTTTGCCA TCCCTAATAA AGCTGGACCT ATCGTCCAAC 
CTCCTGTCGT CTTTTCCTAT AACTGGGTTA CATGGTTTAA CTCACTTAAA ATTAACAGGA 
AATCATGCCT TACAGAGCTT GATATCATCT GAAAACTTTC CAGAACTCAA GGTTATAGAA 
ATGCCTTATG CTTACCAGTG CTGTGCATTT GGAGTGTGTG AGAATGCCTA TAAGATTTCT 
AATCAATGGA ATAAAGGTGA CAACAGCAGT ATGGACGACC TTCATAAGAA AGATGCTGGA 
ATGTTTCAGG CTCAAGATGA ACGTGACCTT GAAGATTTCC TGCTTGACTT TGAGGAAGAC 
CTGAAAGCCC TTCATTCAGT GCAGTGTTCA CCTTCCCCAG GCCCCTTCAA ACCCTGTGAA 
CACCTGCTTG ATGGCTGGCT GATCAGAATT GGAGTGTGGA CCATAGCAGT TCTGGCACTT 
ACTTGTAATG CTTTGGTGAC TTCAACAGTT TTCAGATCCC CTCTGTACAT TTCCCCCATT 
AAACTGTTAA TTGGGGTCAT CGCAGCAGTG AACATGCTCA CGGGAGTCTC CAGTGCCGTG 
CTGGCTGGTG TGGATGCGTT CACTTTTGGC AGCTTTGCAC GACATGGTGC CTGGTGGGAG 
AATGGGGTTG GTTGCCATGT CATTGGTTTT TTGTCCATTT TTGCTTCAGA ATCATCTGTT 
TTCCTGCTTA CTCTGGCAGC CCTGGAGCGT GGGTTCTCTG TGAAATATTC TGCAAAATTT 
GAAACGAAAG CTCCATTTTC TAGCCTGAAA GTAATCATTT TGCTCTGTGC CCTGCTGGCC 
TTGACCATGG CCGCAGTTCC CCTGCTGGGT GGCAGCAAGT ATGGCGCCTC CCCTCTCTGC 
CTGCCTTTGC CTTTTGGGGA GCCCAGCACC ATGGGCTACA TGGTCGCTCT CATCTTGCTC 
AATTCCCTTT GCTTCCTCAT GATGACCATT GCCTACACCA AGCTCTACTG CAATTTGGAC 
AAGGGAGACC TGGAGAATAT TTGGGACTGC TCTATGGTAA AACACATTGC CCTGTTGCTC 
TTCACCAACT GCATCCTAAA CTGCCCTGTG GCTTTCTTGT CCTTCTCCTC TTTAATAAAC 
CTTACATTTA TCAGTCCTGA AGTAATTAAG TTTATCCTTC TGGTGGTAGT CCCACTTCCT 
GCATGTCTCA ATCCCCTTCT CTACATCTTG TTCAATCCTC ACTTTAAGGA GGATCTGGTG 
AGCCTGAGAA AGCAAACCTA CGTCTGGACA AGATCAAAAC ACCCAAGCTT GATGTCAATT 
AACTCTGATG ATGTCGAAAA ACAGTCCTGT GACTCAACTC AAGCCTTGGT AACCTTTACC 
AGCTCCAGCA TCACTTATGA CCTGCCTCCC AGTTCCGTGC CATCACCAGC TTATCCAGTG 
ACTGAGAGCT GCCATCTTTC CTCTGTGGCA TTTGTCCCAT GTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264156.1
CDS	284..2791
Translation	MDTSRLGVLLSLPVLLQLATGGSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIRIGVWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHGAWWENGVGCHVIGFLSIFASESSVFLLTLAALERGFSVKYSAKFETKAPFSSLKVIILLCALLALTMAAVPLLGGSKYGASPLCLPLPFGEPSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLENIWDCSMVKHIALLLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL

Target ORF information:

RefSeq Version	NM_001277227.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277227.2

ORF Insert Sequence:

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTAC ATCTCCATAA CAATAGAATC CACTCCCTGG GAAAGAAATG CTTTGATGGG 
CTCCACAGCC TAGAGACTTT AGATTTAAAT TACAATAACC TTGATGAATT CCCCACTGCA 
ATTAGGACAC TCTCCAACCT TAAAGAACTA GGATTTCATA GCAACAATAT CAGGTCGATA 
CCTGAGAAAG CATTTGTAGG CAACCCTTCT CTTATTACAA TACATTTCTA TGACAATCCC 
ATCCAGTTTG TTGGGAGATC TGCTTTTCAA CATTTACCTG AACTAAGAAC ACTGACTCTG 
AATGGTGCCT CACAAATAAC TGAATTTCCT GATTTAACTG GAACTGCAAA CCTGGAGAGT 
CTGACTTTAA CTGGAGCACA GATCTCATCT CTTCCTCAAA CCGTCTGCAA TCAGTTACCT 
AATCTCCAAG TGCTAGATCT GTCTTACAAC CTATTAGAAG ATTTACCCAG TTTTTCAGTC 
TGCCAAAAGC TTCAGAAAAT TGACCTAAGA CATAATGAAA TCTACGAAAT TAAAGTTGAC 
ACTTTCCAGC AGTTGCTTAG CCTCCGATCG CTGAATTTGG CTTGGAACAA AATTGCTATT 
ATTCACCCCA ATGCATTTTC CACTTTGCCA TCCCTAATAA AGCTGGACCT ATCGTCCAAC 
CTCCTGTCGT CTTTTCCTAT AACTGGGTTA CATGGTTTAA CTCACTTAAA ATTAACAGGA 
AATCATGCCT TACAGAGCTT GATATCATCT GAAAACTTTC CAGAACTCAA GGTTATAGAA 
ATGCCTTATG CTTACCAGTG CTGTGCATTT GGAGTGTGTG AGAATGCCTA TAAGATTTCT 
AATCAATGGA ATAAAGGTGA CAACAGCAGT ATGGACGACC TTCATAAGAA AGATGCTGGA 
ATGTTTCAGG CTCAAGATGA ACGTGACCTT GAAGATTTCC TGCTTGACTT TGAGGAAGAC 
CTGAAAGCCC TTCATTCAGT GCAGTGTTCA CCTTCCCCAG GCCCCTTCAA ACCCTGTGAA 
CACCTGCTTG ATGGCTGGCT GATCAGAATT GGAGTGTGGA CCATAGCAGT TCTGGCACTT 
ACTTGTAATG CTTTGGTGAC TTCAACAGTT TTCAGATCCC CTCTGTACAT TTCCCCCATT 
AAACTGTTAA TTGGGGTCAT CGCAGCAGTG AACATGCTCA CGGGAGTCTC CAGTGCCGTG 
CTGGCTGGTG TGGATGCGTT CACTTTTGGC AGCTTTGCAC GACATGGTGC CTGGTGGGAG 
AATGGGGTTG GTTGCCATGT CATTGGTTTT TTGTCCATTT TTGCTTCAGA ATCATCTGTT 
TTCCTGCTTA CTCTGGCAGC CCTGGAGCGT GGGTTCTCTG TGAAATATTC TGCAAAATTT 
GAAACGAAAG CTCCATTTTC TAGCCTGAAA GTAATCATTT TGCTCTGTGC CCTGCTGGCC 
TTGACCATGG CCGCAGTTCC CCTGCTGGGT GGCAGCAAGT ATGGCGCCTC CCCTCTCTGC 
CTGCCTTTGC CTTTTGGGGA GCCCAGCACC ATGGGCTACA TGGTCGCTCT CATCTTGCTC 
AATTCCCTTT GCTTCCTCAT GATGACCATT GCCTACACCA AGCTCTACTG CAATTTGGAC 
AAGGGAGACC TGGAGAATAT TTGGGACTGC TCTATGGTAA AACACATTGC CCTGTTGCTC 
TTCACCAACT GCATCCTAAA CTGCCCTGTG GCTTTCTTGT CCTTCTCCTC TTTAATAAAC 
CTTACATTTA TCAGTCCTGA AGTAATTAAG TTTATCCTTC TGGTGGTAGT CCCACTTCCT 
GCATGTCTCA ATCCCCTTCT CTACATCTTG TTCAATCCTC ACTTTAAGGA GGATCTGGTG 
AGCCTGAGAA AGCAAACCTA CGTCTGGACA AGATCAAAAC ACCCAAGCTT GATGTCAATT 
AACTCTGATG ATGTCGAAAA ACAGTCCTGT GACTCAACTC AAGCCTTGGT AACCTTTACC 
AGCTCCAGCA TCACTTATGA CCTGCCTCCC AGTTCCGTGC CATCACCAGC TTATCCAGTG 
ACTGAGAGCT GCCATCTTTC CTCTGTGGCA TTTGTCCCAT GTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR5 ( NM_003667.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003658.1 Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21103
Clone ID Related Accession (Same CDS sequence)	NM_003667.4 , NM_003667.3
Accession Version	NM_003667.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR003044.1, AF062006.1, AC078860.19 and CA412916.1. On Nov 23, 2018 this sequence version replaced NM_003667.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.112310.1, AB105190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000266674.10/ ENSP00000266674.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTGC GTCTGGATGC TAACCACATC AGCTATGTGC CCCCAAGCTG TTTCAGTGGC 
CTGCATTCCC TGAGGCACCT GTGGCTGGAT GACAATGCGT TAACAGAAAT CCCCGTCCAG 
GCTTTTAGAA GTTTATCGGC ATTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATACCAGACT ATGCCTTTGG AAACCTCTCC AGCTTGGTAG TTCTACATCT CCATAACAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGGCTCC ACAGCCTAGA GACTTTAGAT 
TTAAATTACA ATAACCTTGA TGAATTCCCC ACTGCAATTA GGACACTCTC CAACCTTAAA 
GAACTAGGAT TTCATAGCAA CAATATCAGG TCGATACCTG AGAAAGCATT TGTAGGCAAC 
CCTTCTCTTA TTACAATACA TTTCTATGAC AATCCCATCC AGTTTGTTGG GAGATCTGCT 
TTTCAACATT TACCTGAACT AAGAACACTG ACTCTGAATG GTGCCTCACA AATAACTGAA 
TTTCCTGATT TAACTGGAAC TGCAAACCTG GAGAGTCTGA CTTTAACTGG AGCACAGATC 
TCATCTCTTC CTCAAACCGT CTGCAATCAG TTACCTAATC TCCAAGTGCT AGATCTGTCT 
TACAACCTAT TAGAAGATTT ACCCAGTTTT TCAGTCTGCC AAAAGCTTCA GAAAATTGAC 
CTAAGACATA ATGAAATCTA CGAAATTAAA GTTGACACTT TCCAGCAGTT GCTTAGCCTC 
CGATCGCTGA ATTTGGCTTG GAACAAAATT GCTATTATTC ACCCCAATGC ATTTTCCACT 
TTGCCATCCC TAATAAAGCT GGACCTATCG TCCAACCTCC TGTCGTCTTT TCCTATAACT 
GGGTTACATG GTTTAACTCA CTTAAAATTA ACAGGAAATC ATGCCTTACA GAGCTTGATA 
TCATCTGAAA ACTTTCCAGA ACTCAAGGTT ATAGAAATGC CTTATGCTTA CCAGTGCTGT 
GCATTTGGAG TGTGTGAGAA TGCCTATAAG ATTTCTAATC AATGGAATAA AGGTGACAAC 
AGCAGTATGG ACGACCTTCA TAAGAAAGAT GCTGGAATGT TTCAGGCTCA AGATGAACGT 
GACCTTGAAG ATTTCCTGCT TGACTTTGAG GAAGACCTGA AAGCCCTTCA TTCAGTGCAG 
TGTTCACCTT CCCCAGGCCC CTTCAAACCC TGTGAACACC TGCTTGATGG CTGGCTGATC 
AGAATTGGAG TGTGGACCAT AGCAGTTCTG GCACTTACTT GTAATGCTTT GGTGACTTCA 
ACAGTTTTCA GATCCCCTCT GTACATTTCC CCCATTAAAC TGTTAATTGG GGTCATCGCA 
GCAGTGAACA TGCTCACGGG AGTCTCCAGT GCCGTGCTGG CTGGTGTGGA TGCGTTCACT 
TTTGGCAGCT TTGCACGACA TGGTGCCTGG TGGGAGAATG GGGTTGGTTG CCATGTCATT 
GGTTTTTTGT CCATTTTTGC TTCAGAATCA TCTGTTTTCC TGCTTACTCT GGCAGCCCTG 
GAGCGTGGGT TCTCTGTGAA ATATTCTGCA AAATTTGAAA CGAAAGCTCC ATTTTCTAGC 
CTGAAAGTAA TCATTTTGCT CTGTGCCCTG CTGGCCTTGA CCATGGCCGC AGTTCCCCTG 
CTGGGTGGCA GCAAGTATGG CGCCTCCCCT CTCTGCCTGC CTTTGCCTTT TGGGGAGCCC 
AGCACCATGG GCTACATGGT CGCTCTCATC TTGCTCAATT CCCTTTGCTT CCTCATGATG 
ACCATTGCCT ACACCAAGCT CTACTGCAAT TTGGACAAGG GAGACCTGGA GAATATTTGG 
GACTGCTCTA TGGTAAAACA CATTGCCCTG TTGCTCTTCA CCAACTGCAT CCTAAACTGC 
CCTGTGGCTT TCTTGTCCTT CTCCTCTTTA ATAAACCTTA CATTTATCAG TCCTGAAGTA 
ATTAAGTTTA TCCTTCTGGT GGTAGTCCCA CTTCCTGCAT GTCTCAATCC CCTTCTCTAC 
ATCTTGTTCA ATCCTCACTT TAAGGAGGAT CTGGTGAGCC TGAGAAAGCA AACCTACGTC 
TGGACAAGAT CAAAACACCC AAGCTTGATG TCAATTAACT CTGATGATGT CGAAAAACAG 
TCCTGTGACT CAACTCAAGC CTTGGTAACC TTTACCAGCT CCAGCATCAC TTATGACCTG 
CCTCCCAGTT CCGTGCCATC ACCAGCTTAT CCAGTGACTG AGAGCTGCCA TCTTTCCTCT 
GTGGCATTTG TCCCATGTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003658.1
CDS	284..3007
Translation	MDTSRLGVLLSLPVLLQLATGGSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIRIGVWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHGAWWENGVGCHVIGFLSIFASESSVFLLTLAALERGFSVKYSAKFETKAPFSSLKVIILLCALLALTMAAVPLLGGSKYGASPLCLPLPFGEPSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLENIWDCSMVKHIALLLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL

Target ORF information:

RefSeq Version	NM_003667.4
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003667.4

ORF Insert Sequence:

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTGC GTCTGGATGC TAACCACATC AGCTATGTGC CCCCAAGCTG TTTCAGTGGC 
CTGCATTCCC TGAGGCACCT GTGGCTGGAT GACAATGCGT TAACAGAAAT CCCCGTCCAG 
GCTTTTAGAA GTTTATCGGC ATTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATACCAGACT ATGCCTTTGG AAACCTCTCC AGCTTGGTAG TTCTACATCT CCATAACAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGGCTCC ACAGCCTAGA GACTTTAGAT 
TTAAATTACA ATAACCTTGA TGAATTCCCC ACTGCAATTA GGACACTCTC CAACCTTAAA 
GAACTAGGAT TTCATAGCAA CAATATCAGG TCGATACCTG AGAAAGCATT TGTAGGCAAC 
CCTTCTCTTA TTACAATACA TTTCTATGAC AATCCCATCC AGTTTGTTGG GAGATCTGCT 
TTTCAACATT TACCTGAACT AAGAACACTG ACTCTGAATG GTGCCTCACA AATAACTGAA 
TTTCCTGATT TAACTGGAAC TGCAAACCTG GAGAGTCTGA CTTTAACTGG AGCACAGATC 
TCATCTCTTC CTCAAACCGT CTGCAATCAG TTACCTAATC TCCAAGTGCT AGATCTGTCT 
TACAACCTAT TAGAAGATTT ACCCAGTTTT TCAGTCTGCC AAAAGCTTCA GAAAATTGAC 
CTAAGACATA ATGAAATCTA CGAAATTAAA GTTGACACTT TCCAGCAGTT GCTTAGCCTC 
CGATCGCTGA ATTTGGCTTG GAACAAAATT GCTATTATTC ACCCCAATGC ATTTTCCACT 
TTGCCATCCC TAATAAAGCT GGACCTATCG TCCAACCTCC TGTCGTCTTT TCCTATAACT 
GGGTTACATG GTTTAACTCA CTTAAAATTA ACAGGAAATC ATGCCTTACA GAGCTTGATA 
TCATCTGAAA ACTTTCCAGA ACTCAAGGTT ATAGAAATGC CTTATGCTTA CCAGTGCTGT 
GCATTTGGAG TGTGTGAGAA TGCCTATAAG ATTTCTAATC AATGGAATAA AGGTGACAAC 
AGCAGTATGG ACGACCTTCA TAAGAAAGAT GCTGGAATGT TTCAGGCTCA AGATGAACGT 
GACCTTGAAG ATTTCCTGCT TGACTTTGAG GAAGACCTGA AAGCCCTTCA TTCAGTGCAG 
TGTTCACCTT CCCCAGGCCC CTTCAAACCC TGTGAACACC TGCTTGATGG CTGGCTGATC 
AGAATTGGAG TGTGGACCAT AGCAGTTCTG GCACTTACTT GTAATGCTTT GGTGACTTCA 
ACAGTTTTCA GATCCCCTCT GTACATTTCC CCCATTAAAC TGTTAATTGG GGTCATCGCA 
GCAGTGAACA TGCTCACGGG AGTCTCCAGT GCCGTGCTGG CTGGTGTGGA TGCGTTCACT 
TTTGGCAGCT TTGCACGACA TGGTGCCTGG TGGGAGAATG GGGTTGGTTG CCATGTCATT 
GGTTTTTTGT CCATTTTTGC TTCAGAATCA TCTGTTTTCC TGCTTACTCT GGCAGCCCTG 
GAGCGTGGGT TCTCTGTGAA ATATTCTGCA AAATTTGAAA CGAAAGCTCC ATTTTCTAGC 
CTGAAAGTAA TCATTTTGCT CTGTGCCCTG CTGGCCTTGA CCATGGCCGC AGTTCCCCTG 
CTGGGTGGCA GCAAGTATGG CGCCTCCCCT CTCTGCCTGC CTTTGCCTTT TGGGGAGCCC 
AGCACCATGG GCTACATGGT CGCTCTCATC TTGCTCAATT CCCTTTGCTT CCTCATGATG 
ACCATTGCCT ACACCAAGCT CTACTGCAAT TTGGACAAGG GAGACCTGGA GAATATTTGG 
GACTGCTCTA TGGTAAAACA CATTGCCCTG TTGCTCTTCA CCAACTGCAT CCTAAACTGC 
CCTGTGGCTT TCTTGTCCTT CTCCTCTTTA ATAAACCTTA CATTTATCAG TCCTGAAGTA 
ATTAAGTTTA TCCTTCTGGT GGTAGTCCCA CTTCCTGCAT GTCTCAATCC CCTTCTCTAC 
ATCTTGTTCA ATCCTCACTT TAAGGAGGAT CTGGTGAGCC TGAGAAAGCA AACCTACGTC 
TGGACAAGAT CAAAACACCC AAGCTTGATG TCAATTAACT CTGATGATGT CGAAAAACAG 
TCCTGTGACT CAACTCAAGC CTTGGTAACC TTTACCAGCT CCAGCATCAC TTATGACCTG 
CCTCCCAGTT CCGTGCCATC ACCAGCTTAT CCAGTGACTG AGAGCTGCCA TCTTTCCTCT 
GTGGCATTTG TCCCATGTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR5 ( NM_003667.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003658.1 Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21103
Clone ID Related Accession (Same CDS sequence)	NM_003667.4 , NM_003667.3
Accession Version	NM_003667.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC323352.1, DR003044.1, AF062006.1, AC078860.19 and CA412916.1. On Mar 21, 2013 this sequence version replaced NM_003667.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB105190.1, SRR1660803.112310.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTGC GTCTGGATGC TAACCACATC AGCTATGTGC CCCCAAGCTG TTTCAGTGGC 
CTGCATTCCC TGAGGCACCT GTGGCTGGAT GACAATGCGT TAACAGAAAT CCCCGTCCAG 
GCTTTTAGAA GTTTATCGGC ATTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATACCAGACT ATGCCTTTGG AAACCTCTCC AGCTTGGTAG TTCTACATCT CCATAACAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGGCTCC ACAGCCTAGA GACTTTAGAT 
TTAAATTACA ATAACCTTGA TGAATTCCCC ACTGCAATTA GGACACTCTC CAACCTTAAA 
GAACTAGGAT TTCATAGCAA CAATATCAGG TCGATACCTG AGAAAGCATT TGTAGGCAAC 
CCTTCTCTTA TTACAATACA TTTCTATGAC AATCCCATCC AGTTTGTTGG GAGATCTGCT 
TTTCAACATT TACCTGAACT AAGAACACTG ACTCTGAATG GTGCCTCACA AATAACTGAA 
TTTCCTGATT TAACTGGAAC TGCAAACCTG GAGAGTCTGA CTTTAACTGG AGCACAGATC 
TCATCTCTTC CTCAAACCGT CTGCAATCAG TTACCTAATC TCCAAGTGCT AGATCTGTCT 
TACAACCTAT TAGAAGATTT ACCCAGTTTT TCAGTCTGCC AAAAGCTTCA GAAAATTGAC 
CTAAGACATA ATGAAATCTA CGAAATTAAA GTTGACACTT TCCAGCAGTT GCTTAGCCTC 
CGATCGCTGA ATTTGGCTTG GAACAAAATT GCTATTATTC ACCCCAATGC ATTTTCCACT 
TTGCCATCCC TAATAAAGCT GGACCTATCG TCCAACCTCC TGTCGTCTTT TCCTATAACT 
GGGTTACATG GTTTAACTCA CTTAAAATTA ACAGGAAATC ATGCCTTACA GAGCTTGATA 
TCATCTGAAA ACTTTCCAGA ACTCAAGGTT ATAGAAATGC CTTATGCTTA CCAGTGCTGT 
GCATTTGGAG TGTGTGAGAA TGCCTATAAG ATTTCTAATC AATGGAATAA AGGTGACAAC 
AGCAGTATGG ACGACCTTCA TAAGAAAGAT GCTGGAATGT TTCAGGCTCA AGATGAACGT 
GACCTTGAAG ATTTCCTGCT TGACTTTGAG GAAGACCTGA AAGCCCTTCA TTCAGTGCAG 
TGTTCACCTT CCCCAGGCCC CTTCAAACCC TGTGAACACC TGCTTGATGG CTGGCTGATC 
AGAATTGGAG TGTGGACCAT AGCAGTTCTG GCACTTACTT GTAATGCTTT GGTGACTTCA 
ACAGTTTTCA GATCCCCTCT GTACATTTCC CCCATTAAAC TGTTAATTGG GGTCATCGCA 
GCAGTGAACA TGCTCACGGG AGTCTCCAGT GCCGTGCTGG CTGGTGTGGA TGCGTTCACT 
TTTGGCAGCT TTGCACGACA TGGTGCCTGG TGGGAGAATG GGGTTGGTTG CCATGTCATT 
GGTTTTTTGT CCATTTTTGC TTCAGAATCA TCTGTTTTCC TGCTTACTCT GGCAGCCCTG 
GAGCGTGGGT TCTCTGTGAA ATATTCTGCA AAATTTGAAA CGAAAGCTCC ATTTTCTAGC 
CTGAAAGTAA TCATTTTGCT CTGTGCCCTG CTGGCCTTGA CCATGGCCGC AGTTCCCCTG 
CTGGGTGGCA GCAAGTATGG CGCCTCCCCT CTCTGCCTGC CTTTGCCTTT TGGGGAGCCC 
AGCACCATGG GCTACATGGT CGCTCTCATC TTGCTCAATT CCCTTTGCTT CCTCATGATG 
ACCATTGCCT ACACCAAGCT CTACTGCAAT TTGGACAAGG GAGACCTGGA GAATATTTGG 
GACTGCTCTA TGGTAAAACA CATTGCCCTG TTGCTCTTCA CCAACTGCAT CCTAAACTGC 
CCTGTGGCTT TCTTGTCCTT CTCCTCTTTA ATAAACCTTA CATTTATCAG TCCTGAAGTA 
ATTAAGTTTA TCCTTCTGGT GGTAGTCCCA CTTCCTGCAT GTCTCAATCC CCTTCTCTAC 
ATCTTGTTCA ATCCTCACTT TAAGGAGGAT CTGGTGAGCC TGAGAAAGCA AACCTACGTC 
TGGACAAGAT CAAAACACCC AAGCTTGATG TCAATTAACT CTGATGATGT CGAAAAACAG 
TCCTGTGACT CAACTCAAGC CTTGGTAACC TTTACCAGCT CCAGCATCAC TTATGACCTG 
CCTCCCAGTT CCGTGCCATC ACCAGCTTAT CCAGTGACTG AGAGCTGCCA TCTTTCCTCT 
GTGGCATTTG TCCCATGTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003658.1
CDS	312..3035
Translation	MDTSRLGVLLSLPVLLQLATGGSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIRIGVWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHGAWWENGVGCHVIGFLSIFASESSVFLLTLAALERGFSVKYSAKFETKAPFSSLKVIILLCALLALTMAAVPLLGGSKYGASPLCLPLPFGEPSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLENIWDCSMVKHIALLLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL

Target ORF information:

RefSeq Version	NM_003667.3
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003667.3

ORF Insert Sequence:

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTGC GTCTGGATGC TAACCACATC AGCTATGTGC CCCCAAGCTG TTTCAGTGGC 
CTGCATTCCC TGAGGCACCT GTGGCTGGAT GACAATGCGT TAACAGAAAT CCCCGTCCAG 
GCTTTTAGAA GTTTATCGGC ATTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATACCAGACT ATGCCTTTGG AAACCTCTCC AGCTTGGTAG TTCTACATCT CCATAACAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGGCTCC ACAGCCTAGA GACTTTAGAT 
TTAAATTACA ATAACCTTGA TGAATTCCCC ACTGCAATTA GGACACTCTC CAACCTTAAA 
GAACTAGGAT TTCATAGCAA CAATATCAGG TCGATACCTG AGAAAGCATT TGTAGGCAAC 
CCTTCTCTTA TTACAATACA TTTCTATGAC AATCCCATCC AGTTTGTTGG GAGATCTGCT 
TTTCAACATT TACCTGAACT AAGAACACTG ACTCTGAATG GTGCCTCACA AATAACTGAA 
TTTCCTGATT TAACTGGAAC TGCAAACCTG GAGAGTCTGA CTTTAACTGG AGCACAGATC 
TCATCTCTTC CTCAAACCGT CTGCAATCAG TTACCTAATC TCCAAGTGCT AGATCTGTCT 
TACAACCTAT TAGAAGATTT ACCCAGTTTT TCAGTCTGCC AAAAGCTTCA GAAAATTGAC 
CTAAGACATA ATGAAATCTA CGAAATTAAA GTTGACACTT TCCAGCAGTT GCTTAGCCTC 
CGATCGCTGA ATTTGGCTTG GAACAAAATT GCTATTATTC ACCCCAATGC ATTTTCCACT 
TTGCCATCCC TAATAAAGCT GGACCTATCG TCCAACCTCC TGTCGTCTTT TCCTATAACT 
GGGTTACATG GTTTAACTCA CTTAAAATTA ACAGGAAATC ATGCCTTACA GAGCTTGATA 
TCATCTGAAA ACTTTCCAGA ACTCAAGGTT ATAGAAATGC CTTATGCTTA CCAGTGCTGT 
GCATTTGGAG TGTGTGAGAA TGCCTATAAG ATTTCTAATC AATGGAATAA AGGTGACAAC 
AGCAGTATGG ACGACCTTCA TAAGAAAGAT GCTGGAATGT TTCAGGCTCA AGATGAACGT 
GACCTTGAAG ATTTCCTGCT TGACTTTGAG GAAGACCTGA AAGCCCTTCA TTCAGTGCAG 
TGTTCACCTT CCCCAGGCCC CTTCAAACCC TGTGAACACC TGCTTGATGG CTGGCTGATC 
AGAATTGGAG TGTGGACCAT AGCAGTTCTG GCACTTACTT GTAATGCTTT GGTGACTTCA 
ACAGTTTTCA GATCCCCTCT GTACATTTCC CCCATTAAAC TGTTAATTGG GGTCATCGCA 
GCAGTGAACA TGCTCACGGG AGTCTCCAGT GCCGTGCTGG CTGGTGTGGA TGCGTTCACT 
TTTGGCAGCT TTGCACGACA TGGTGCCTGG TGGGAGAATG GGGTTGGTTG CCATGTCATT 
GGTTTTTTGT CCATTTTTGC TTCAGAATCA TCTGTTTTCC TGCTTACTCT GGCAGCCCTG 
GAGCGTGGGT TCTCTGTGAA ATATTCTGCA AAATTTGAAA CGAAAGCTCC ATTTTCTAGC 
CTGAAAGTAA TCATTTTGCT CTGTGCCCTG CTGGCCTTGA CCATGGCCGC AGTTCCCCTG 
CTGGGTGGCA GCAAGTATGG CGCCTCCCCT CTCTGCCTGC CTTTGCCTTT TGGGGAGCCC 
AGCACCATGG GCTACATGGT CGCTCTCATC TTGCTCAATT CCCTTTGCTT CCTCATGATG 
ACCATTGCCT ACACCAAGCT CTACTGCAAT TTGGACAAGG GAGACCTGGA GAATATTTGG 
GACTGCTCTA TGGTAAAACA CATTGCCCTG TTGCTCTTCA CCAACTGCAT CCTAAACTGC 
CCTGTGGCTT TCTTGTCCTT CTCCTCTTTA ATAAACCTTA CATTTATCAG TCCTGAAGTA 
ATTAAGTTTA TCCTTCTGGT GGTAGTCCCA CTTCCTGCAT GTCTCAATCC CCTTCTCTAC 
ATCTTGTTCA ATCCTCACTT TAAGGAGGAT CTGGTGAGCC TGAGAAAGCA AACCTACGTC 
TGGACAAGAT CAAAACACCC AAGCTTGATG TCAATTAACT CTGATGATGT CGAAAAACAG 
TCCTGTGACT CAACTCAAGC CTTGGTAACC TTTACCAGCT CCAGCATCAC TTATGACCTG 
CCTCCCAGTT CCGTGCCATC ACCAGCTTAT CCAGTGACTG AGAGCTGCCA TCTTTCCTCT 
GTGGCATTTG TCCCATGTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR5 ( NM_001277226.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264155.1 Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21443
Clone ID Related Accession (Same CDS sequence)	NM_001277226.1 , NM_001277226.2
Accession Version	NM_001277226.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC323352.1, DR003044.1, BC096325.1, AC078860.19 and CA412916.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.232375.1, SRR1803612.206295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTGC GTCTGGATGC TAACCACATC AGCTATGTGC CCCCAAGCTG TTTCAGTGGC 
CTGCATTCCC TGAGGCACCT GTGGCTGGAT GACAATGCGT TAACAGAAAT CCCCGTCCAG 
GCTTTTAGAA GTTTATCGGC ATTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATACCAGACT ATGCCTTTGG AAACCTCTCC AGCTTGGTAG TTCTACATCT CCATAACAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGGCTCC ACAGCCTAGA GACTTTAGAT 
TTAAATTACA ATAACCTTGA TGAATTCCCC ACTGCAATTA GGACACTCTC CAACCTTAAA 
GAACTACATT TCTATGACAA TCCCATCCAG TTTGTTGGGA GATCTGCTTT TCAACATTTA 
CCTGAACTAA GAACACTGAC TCTGAATGGT GCCTCACAAA TAACTGAATT TCCTGATTTA 
ACTGGAACTG CAAACCTGGA GAGTCTGACT TTAACTGGAG CACAGATCTC ATCTCTTCCT 
CAAACCGTCT GCAATCAGTT ACCTAATCTC CAAGTGCTAG ATCTGTCTTA CAACCTATTA 
GAAGATTTAC CCAGTTTTTC AGTCTGCCAA AAGCTTCAGA AAATTGACCT AAGACATAAT 
GAAATCTACG AAATTAAAGT TGACACTTTC CAGCAGTTGC TTAGCCTCCG ATCGCTGAAT 
TTGGCTTGGA ACAAAATTGC TATTATTCAC CCCAATGCAT TTTCCACTTT GCCATCCCTA 
ATAAAGCTGG ACCTATCGTC CAACCTCCTG TCGTCTTTTC CTATAACTGG GTTACATGGT 
TTAACTCACT TAAAATTAAC AGGAAATCAT GCCTTACAGA GCTTGATATC ATCTGAAAAC 
TTTCCAGAAC TCAAGGTTAT AGAAATGCCT TATGCTTACC AGTGCTGTGC ATTTGGAGTG 
TGTGAGAATG CCTATAAGAT TTCTAATCAA TGGAATAAAG GTGACAACAG CAGTATGGAC 
GACCTTCATA AGAAAGATGC TGGAATGTTT CAGGCTCAAG ATGAACGTGA CCTTGAAGAT 
TTCCTGCTTG ACTTTGAGGA AGACCTGAAA GCCCTTCATT CAGTGCAGTG TTCACCTTCC 
CCAGGCCCCT TCAAACCCTG TGAACACCTG CTTGATGGCT GGCTGATCAG AATTGGAGTG 
TGGACCATAG CAGTTCTGGC ACTTACTTGT AATGCTTTGG TGACTTCAAC AGTTTTCAGA 
TCCCCTCTGT ACATTTCCCC CATTAAACTG TTAATTGGGG TCATCGCAGC AGTGAACATG 
CTCACGGGAG TCTCCAGTGC CGTGCTGGCT GGTGTGGATG CGTTCACTTT TGGCAGCTTT 
GCACGACATG GTGCCTGGTG GGAGAATGGG GTTGGTTGCC ATGTCATTGG TTTTTTGTCC 
ATTTTTGCTT CAGAATCATC TGTTTTCCTG CTTACTCTGG CAGCCCTGGA GCGTGGGTTC 
TCTGTGAAAT ATTCTGCAAA ATTTGAAACG AAAGCTCCAT TTTCTAGCCT GAAAGTAATC 
ATTTTGCTCT GTGCCCTGCT GGCCTTGACC ATGGCCGCAG TTCCCCTGCT GGGTGGCAGC 
AAGTATGGCG CCTCCCCTCT CTGCCTGCCT TTGCCTTTTG GGGAGCCCAG CACCATGGGC 
TACATGGTCG CTCTCATCTT GCTCAATTCC CTTTGCTTCC TCATGATGAC CATTGCCTAC 
ACCAAGCTCT ACTGCAATTT GGACAAGGGA GACCTGGAGA ATATTTGGGA CTGCTCTATG 
GTAAAACACA TTGCCCTGTT GCTCTTCACC AACTGCATCC TAAACTGCCC TGTGGCTTTC 
TTGTCCTTCT CCTCTTTAAT AAACCTTACA TTTATCAGTC CTGAAGTAAT TAAGTTTATC 
CTTCTGGTGG TAGTCCCACT TCCTGCATGT CTCAATCCCC TTCTCTACAT CTTGTTCAAT 
CCTCACTTTA AGGAGGATCT GGTGAGCCTG AGAAAGCAAA CCTACGTCTG GACAAGATCA 
AAACACCCAA GCTTGATGTC AATTAACTCT GATGATGTCG AAAAACAGTC CTGTGACTCA 
ACTCAAGCCT TGGTAACCTT TACCAGCTCC AGCATCACTT ATGACCTGCC TCCCAGTTCC 
GTGCCATCAC CAGCTTATCC AGTGACTGAG AGCTGCCATC TTTCCTCTGT GGCATTTGTC 
CCATGTCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264155.1
CDS	312..2963
Translation	MDTSRLGVLLSLPVLLQLATGGSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIRIGVWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHGAWWENGVGCHVIGFLSIFASESSVFLLTLAALERGFSVKYSAKFETKAPFSSLKVIILLCALLALTMAAVPLLGGSKYGASPLCLPLPFGEPSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLENIWDCSMVKHIALLLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL

Target ORF information:

RefSeq Version	NM_001277226.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277226.1

ORF Insert Sequence:

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTGC GTCTGGATGC TAACCACATC AGCTATGTGC CCCCAAGCTG TTTCAGTGGC 
CTGCATTCCC TGAGGCACCT GTGGCTGGAT GACAATGCGT TAACAGAAAT CCCCGTCCAG 
GCTTTTAGAA GTTTATCGGC ATTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATACCAGACT ATGCCTTTGG AAACCTCTCC AGCTTGGTAG TTCTACATCT CCATAACAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGGCTCC ACAGCCTAGA GACTTTAGAT 
TTAAATTACA ATAACCTTGA TGAATTCCCC ACTGCAATTA GGACACTCTC CAACCTTAAA 
GAACTACATT TCTATGACAA TCCCATCCAG TTTGTTGGGA GATCTGCTTT TCAACATTTA 
CCTGAACTAA GAACACTGAC TCTGAATGGT GCCTCACAAA TAACTGAATT TCCTGATTTA 
ACTGGAACTG CAAACCTGGA GAGTCTGACT TTAACTGGAG CACAGATCTC ATCTCTTCCT 
CAAACCGTCT GCAATCAGTT ACCTAATCTC CAAGTGCTAG ATCTGTCTTA CAACCTATTA 
GAAGATTTAC CCAGTTTTTC AGTCTGCCAA AAGCTTCAGA AAATTGACCT AAGACATAAT 
GAAATCTACG AAATTAAAGT TGACACTTTC CAGCAGTTGC TTAGCCTCCG ATCGCTGAAT 
TTGGCTTGGA ACAAAATTGC TATTATTCAC CCCAATGCAT TTTCCACTTT GCCATCCCTA 
ATAAAGCTGG ACCTATCGTC CAACCTCCTG TCGTCTTTTC CTATAACTGG GTTACATGGT 
TTAACTCACT TAAAATTAAC AGGAAATCAT GCCTTACAGA GCTTGATATC ATCTGAAAAC 
TTTCCAGAAC TCAAGGTTAT AGAAATGCCT TATGCTTACC AGTGCTGTGC ATTTGGAGTG 
TGTGAGAATG CCTATAAGAT TTCTAATCAA TGGAATAAAG GTGACAACAG CAGTATGGAC 
GACCTTCATA AGAAAGATGC TGGAATGTTT CAGGCTCAAG ATGAACGTGA CCTTGAAGAT 
TTCCTGCTTG ACTTTGAGGA AGACCTGAAA GCCCTTCATT CAGTGCAGTG TTCACCTTCC 
CCAGGCCCCT TCAAACCCTG TGAACACCTG CTTGATGGCT GGCTGATCAG AATTGGAGTG 
TGGACCATAG CAGTTCTGGC ACTTACTTGT AATGCTTTGG TGACTTCAAC AGTTTTCAGA 
TCCCCTCTGT ACATTTCCCC CATTAAACTG TTAATTGGGG TCATCGCAGC AGTGAACATG 
CTCACGGGAG TCTCCAGTGC CGTGCTGGCT GGTGTGGATG CGTTCACTTT TGGCAGCTTT 
GCACGACATG GTGCCTGGTG GGAGAATGGG GTTGGTTGCC ATGTCATTGG TTTTTTGTCC 
ATTTTTGCTT CAGAATCATC TGTTTTCCTG CTTACTCTGG CAGCCCTGGA GCGTGGGTTC 
TCTGTGAAAT ATTCTGCAAA ATTTGAAACG AAAGCTCCAT TTTCTAGCCT GAAAGTAATC 
ATTTTGCTCT GTGCCCTGCT GGCCTTGACC ATGGCCGCAG TTCCCCTGCT GGGTGGCAGC 
AAGTATGGCG CCTCCCCTCT CTGCCTGCCT TTGCCTTTTG GGGAGCCCAG CACCATGGGC 
TACATGGTCG CTCTCATCTT GCTCAATTCC CTTTGCTTCC TCATGATGAC CATTGCCTAC 
ACCAAGCTCT ACTGCAATTT GGACAAGGGA GACCTGGAGA ATATTTGGGA CTGCTCTATG 
GTAAAACACA TTGCCCTGTT GCTCTTCACC AACTGCATCC TAAACTGCCC TGTGGCTTTC 
TTGTCCTTCT CCTCTTTAAT AAACCTTACA TTTATCAGTC CTGAAGTAAT TAAGTTTATC 
CTTCTGGTGG TAGTCCCACT TCCTGCATGT CTCAATCCCC TTCTCTACAT CTTGTTCAAT 
CCTCACTTTA AGGAGGATCT GGTGAGCCTG AGAAAGCAAA CCTACGTCTG GACAAGATCA 
AAACACCCAA GCTTGATGTC AATTAACTCT GATGATGTCG AAAAACAGTC CTGTGACTCA 
ACTCAAGCCT TGGTAACCTT TACCAGCTCC AGCATCACTT ATGACCTGCC TCCCAGTTCC 
GTGCCATCAC CAGCTTATCC AGTGACTGAG AGCTGCCATC TTTCCTCTGT GGCATTTGTC 
CCATGTCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR5 ( NM_001277226.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264155.1 Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21443
Clone ID Related Accession (Same CDS sequence)	NM_001277226.1 , NM_001277226.2
Accession Version	NM_001277226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR003044.1, BC096325.1, AC078860.19 and CA412916.1. On May 31, 2019 this sequence version replaced NM_001277226.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.281631.1, SRR1803613.232375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTGC GTCTGGATGC TAACCACATC AGCTATGTGC CCCCAAGCTG TTTCAGTGGC 
CTGCATTCCC TGAGGCACCT GTGGCTGGAT GACAATGCGT TAACAGAAAT CCCCGTCCAG 
GCTTTTAGAA GTTTATCGGC ATTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATACCAGACT ATGCCTTTGG AAACCTCTCC AGCTTGGTAG TTCTACATCT CCATAACAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGGCTCC ACAGCCTAGA GACTTTAGAT 
TTAAATTACA ATAACCTTGA TGAATTCCCC ACTGCAATTA GGACACTCTC CAACCTTAAA 
GAACTACATT TCTATGACAA TCCCATCCAG TTTGTTGGGA GATCTGCTTT TCAACATTTA 
CCTGAACTAA GAACACTGAC TCTGAATGGT GCCTCACAAA TAACTGAATT TCCTGATTTA 
ACTGGAACTG CAAACCTGGA GAGTCTGACT TTAACTGGAG CACAGATCTC ATCTCTTCCT 
CAAACCGTCT GCAATCAGTT ACCTAATCTC CAAGTGCTAG ATCTGTCTTA CAACCTATTA 
GAAGATTTAC CCAGTTTTTC AGTCTGCCAA AAGCTTCAGA AAATTGACCT AAGACATAAT 
GAAATCTACG AAATTAAAGT TGACACTTTC CAGCAGTTGC TTAGCCTCCG ATCGCTGAAT 
TTGGCTTGGA ACAAAATTGC TATTATTCAC CCCAATGCAT TTTCCACTTT GCCATCCCTA 
ATAAAGCTGG ACCTATCGTC CAACCTCCTG TCGTCTTTTC CTATAACTGG GTTACATGGT 
TTAACTCACT TAAAATTAAC AGGAAATCAT GCCTTACAGA GCTTGATATC ATCTGAAAAC 
TTTCCAGAAC TCAAGGTTAT AGAAATGCCT TATGCTTACC AGTGCTGTGC ATTTGGAGTG 
TGTGAGAATG CCTATAAGAT TTCTAATCAA TGGAATAAAG GTGACAACAG CAGTATGGAC 
GACCTTCATA AGAAAGATGC TGGAATGTTT CAGGCTCAAG ATGAACGTGA CCTTGAAGAT 
TTCCTGCTTG ACTTTGAGGA AGACCTGAAA GCCCTTCATT CAGTGCAGTG TTCACCTTCC 
CCAGGCCCCT TCAAACCCTG TGAACACCTG CTTGATGGCT GGCTGATCAG AATTGGAGTG 
TGGACCATAG CAGTTCTGGC ACTTACTTGT AATGCTTTGG TGACTTCAAC AGTTTTCAGA 
TCCCCTCTGT ACATTTCCCC CATTAAACTG TTAATTGGGG TCATCGCAGC AGTGAACATG 
CTCACGGGAG TCTCCAGTGC CGTGCTGGCT GGTGTGGATG CGTTCACTTT TGGCAGCTTT 
GCACGACATG GTGCCTGGTG GGAGAATGGG GTTGGTTGCC ATGTCATTGG TTTTTTGTCC 
ATTTTTGCTT CAGAATCATC TGTTTTCCTG CTTACTCTGG CAGCCCTGGA GCGTGGGTTC 
TCTGTGAAAT ATTCTGCAAA ATTTGAAACG AAAGCTCCAT TTTCTAGCCT GAAAGTAATC 
ATTTTGCTCT GTGCCCTGCT GGCCTTGACC ATGGCCGCAG TTCCCCTGCT GGGTGGCAGC 
AAGTATGGCG CCTCCCCTCT CTGCCTGCCT TTGCCTTTTG GGGAGCCCAG CACCATGGGC 
TACATGGTCG CTCTCATCTT GCTCAATTCC CTTTGCTTCC TCATGATGAC CATTGCCTAC 
ACCAAGCTCT ACTGCAATTT GGACAAGGGA GACCTGGAGA ATATTTGGGA CTGCTCTATG 
GTAAAACACA TTGCCCTGTT GCTCTTCACC AACTGCATCC TAAACTGCCC TGTGGCTTTC 
TTGTCCTTCT CCTCTTTAAT AAACCTTACA TTTATCAGTC CTGAAGTAAT TAAGTTTATC 
CTTCTGGTGG TAGTCCCACT TCCTGCATGT CTCAATCCCC TTCTCTACAT CTTGTTCAAT 
CCTCACTTTA AGGAGGATCT GGTGAGCCTG AGAAAGCAAA CCTACGTCTG GACAAGATCA 
AAACACCCAA GCTTGATGTC AATTAACTCT GATGATGTCG AAAAACAGTC CTGTGACTCA 
ACTCAAGCCT TGGTAACCTT TACCAGCTCC AGCATCACTT ATGACCTGCC TCCCAGTTCC 
GTGCCATCAC CAGCTTATCC AGTGACTGAG AGCTGCCATC TTTCCTCTGT GGCATTTGTC 
CCATGTCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264155.1
CDS	284..2935
Translation	MDTSRLGVLLSLPVLLQLATGGSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIRIGVWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHGAWWENGVGCHVIGFLSIFASESSVFLLTLAALERGFSVKYSAKFETKAPFSSLKVIILLCALLALTMAAVPLLGGSKYGASPLCLPLPFGEPSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLENIWDCSMVKHIALLLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL

Target ORF information:

RefSeq Version	NM_001277226.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277226.2

ORF Insert Sequence:

ATGGACACCT CCCGGCTCGG TGTGCTCCTG TCCTTGCCTG TGCTGCTGCA GCTGGCGACC 
GGGGGCAGCT CTCCCAGGTC TGGTGTGTTG CTGAGGGGCT GCCCCACACA CTGTCATTGC 
GAGCCCGACG GCAGGATGTT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCTTCCAACC TCAGCGTCTT CACCTCCTAC CTAGACCTCA GTATGAACAA CATCAGTCAG 
CTGCTCCCGA ATCCCCTGCC CAGTCTCCGC TTCCTGGAGG AGTTACGTCT TGCGGGAAAC 
GCTCTGACAT ACATTCCCAA GGGAGCATTC ACTGGCCTTT ACAGTCTTAA AGTTCTTATG 
CTGCAGAATA ATCAGCTAAG ACACGTACCC ACAGAAGCTC TGCAGAATTT GCGAAGCCTT 
CAATCCCTGC GTCTGGATGC TAACCACATC AGCTATGTGC CCCCAAGCTG TTTCAGTGGC 
CTGCATTCCC TGAGGCACCT GTGGCTGGAT GACAATGCGT TAACAGAAAT CCCCGTCCAG 
GCTTTTAGAA GTTTATCGGC ATTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATACCAGACT ATGCCTTTGG AAACCTCTCC AGCTTGGTAG TTCTACATCT CCATAACAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGGCTCC ACAGCCTAGA GACTTTAGAT 
TTAAATTACA ATAACCTTGA TGAATTCCCC ACTGCAATTA GGACACTCTC CAACCTTAAA 
GAACTACATT TCTATGACAA TCCCATCCAG TTTGTTGGGA GATCTGCTTT TCAACATTTA 
CCTGAACTAA GAACACTGAC TCTGAATGGT GCCTCACAAA TAACTGAATT TCCTGATTTA 
ACTGGAACTG CAAACCTGGA GAGTCTGACT TTAACTGGAG CACAGATCTC ATCTCTTCCT 
CAAACCGTCT GCAATCAGTT ACCTAATCTC CAAGTGCTAG ATCTGTCTTA CAACCTATTA 
GAAGATTTAC CCAGTTTTTC AGTCTGCCAA AAGCTTCAGA AAATTGACCT AAGACATAAT 
GAAATCTACG AAATTAAAGT TGACACTTTC CAGCAGTTGC TTAGCCTCCG ATCGCTGAAT 
TTGGCTTGGA ACAAAATTGC TATTATTCAC CCCAATGCAT TTTCCACTTT GCCATCCCTA 
ATAAAGCTGG ACCTATCGTC CAACCTCCTG TCGTCTTTTC CTATAACTGG GTTACATGGT 
TTAACTCACT TAAAATTAAC AGGAAATCAT GCCTTACAGA GCTTGATATC ATCTGAAAAC 
TTTCCAGAAC TCAAGGTTAT AGAAATGCCT TATGCTTACC AGTGCTGTGC ATTTGGAGTG 
TGTGAGAATG CCTATAAGAT TTCTAATCAA TGGAATAAAG GTGACAACAG CAGTATGGAC 
GACCTTCATA AGAAAGATGC TGGAATGTTT CAGGCTCAAG ATGAACGTGA CCTTGAAGAT 
TTCCTGCTTG ACTTTGAGGA AGACCTGAAA GCCCTTCATT CAGTGCAGTG TTCACCTTCC 
CCAGGCCCCT TCAAACCCTG TGAACACCTG CTTGATGGCT GGCTGATCAG AATTGGAGTG 
TGGACCATAG CAGTTCTGGC ACTTACTTGT AATGCTTTGG TGACTTCAAC AGTTTTCAGA 
TCCCCTCTGT ACATTTCCCC CATTAAACTG TTAATTGGGG TCATCGCAGC AGTGAACATG 
CTCACGGGAG TCTCCAGTGC CGTGCTGGCT GGTGTGGATG CGTTCACTTT TGGCAGCTTT 
GCACGACATG GTGCCTGGTG GGAGAATGGG GTTGGTTGCC ATGTCATTGG TTTTTTGTCC 
ATTTTTGCTT CAGAATCATC TGTTTTCCTG CTTACTCTGG CAGCCCTGGA GCGTGGGTTC 
TCTGTGAAAT ATTCTGCAAA ATTTGAAACG AAAGCTCCAT TTTCTAGCCT GAAAGTAATC 
ATTTTGCTCT GTGCCCTGCT GGCCTTGACC ATGGCCGCAG TTCCCCTGCT GGGTGGCAGC 
AAGTATGGCG CCTCCCCTCT CTGCCTGCCT TTGCCTTTTG GGGAGCCCAG CACCATGGGC 
TACATGGTCG CTCTCATCTT GCTCAATTCC CTTTGCTTCC TCATGATGAC CATTGCCTAC 
ACCAAGCTCT ACTGCAATTT GGACAAGGGA GACCTGGAGA ATATTTGGGA CTGCTCTATG 
GTAAAACACA TTGCCCTGTT GCTCTTCACC AACTGCATCC TAAACTGCCC TGTGGCTTTC 
TTGTCCTTCT CCTCTTTAAT AAACCTTACA TTTATCAGTC CTGAAGTAAT TAAGTTTATC 
CTTCTGGTGG TAGTCCCACT TCCTGCATGT CTCAATCCCC TTCTCTACAT CTTGTTCAAT 
CCTCACTTTA AGGAGGATCT GGTGAGCCTG AGAAAGCAAA CCTACGTCTG GACAAGATCA 
AAACACCCAA GCTTGATGTC AATTAACTCT GATGATGTCG AAAAACAGTC CTGTGACTCA 
ACTCAAGCCT TGGTAACCTT TACCAGCTCC AGCATCACTT ATGACCTGCC TCCCAGTTCC 
GTGCCATCAC CAGCTTATCC AGTGACTGAG AGCTGCCATC TTTCCTCTGT GGCATTTGTC 
CCATGTCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LGR5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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