SELENOI cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SELENOI
Entrez Gene ID	85465
Full Name	selenoprotein I
Synonyms	EPT1,SELI,SEPI
General protein information	Preferred Names selenoprotein I Names ethanolaminephosphotransferase 1 ethanolaminephosphotransferase 1 (CDP-ethanolamine-specific) hEPT1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.3
Summary	The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016].
Disorder MIM:	607915

mRNA and Protein(s)

mRNA	Protein	Name
NM_033505.2	NP_277040.1	ethanolaminephosphotransferase 1
NM_033505.4	NP_277040.1	ethanolaminephosphotransferase 1
NM_033505.3	NP_277040.1	ethanolaminephosphotransferase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EPT1	NP_001140169.1
	EPT1	NP_001129035.1
Gallus gallus (chicken)	EPT1	NP_001026699.2
Caenorhabditis elegans (roundworm)	CELE_F54D7.2	NP_491454.2
Mus musculus (house mouse)	Ept1	NP_081928.2
Rattus norvegicus (Norway rat)	Seli	NP_001128226.2
Drosophila melanogaster (fruit fly)	CG33116	NP_788074.1
Xenopus tropicalis (tropical clawed frog)	ept1	NP_001004832.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008881	XP_319628.3
Homo sapiens (human)	EPT1	NP_277040.1
Canis lupus familiaris (dog)	EPT1	NP_001157958.1
Bos taurus (cattle)	EPT1	NP_001068725.2

Gene Ontology
Bibliography

Related articles in PubMed

Cloning, intracellular localization, and expression of the mammalian selenocysteine-containing protein SELENOI (SelI) in tumor cell lines.
Varlamova EG, Goltyaev MV, Novoselov VI, Fesenko EE
Doklady. Biochemistry and biophysics476(1)320-322(2017 Sep)

A mutation of EPT1 (SELENOI) underlies a new disorder of Kennedy pathway phospholipid biosynthesis.
Ahmed MY, Al-Khayat A, Al-Murshedi F, Al-Futaisi A, Chioza BA, Pedro Fernandez-Murray J, Self JE, Salter CG, Harlalka GV, Rawlins LE, Al-Zuhaibi S, Al-Azri F, Al-Rashdi F, Cazenave-Gassiot A, Wenk MR, Al-Salmi F, Patton MA, Silver DL, Baple EL, McMaster CR, Crosby AH
Brain : a journal of neurology140(3)547-554(2017 Mar)

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Oshikawa M, Tsutsui C, Ikegami T, Fuchida Y, Matsubara M, Toyama S, Usami R, Ohtoko K, Kato S
Investigative ophthalmology & visual science52(9)6662-70(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

intracellular localization of human selenoprotein SelI and the degree of expression of its gene in different human tumor cell lines
Title: Cloning, intracellular localization, and expression of the mammalian selenocysteine-containing protein SELENOI (SelI) in tumor cell lines.

These studies define the first human disorder arising due to defective CDP-ethanolamine biosynthesis and provide new insight into the role of Kennedy pathway components in human neurological function
Title: A mutation of EPT1 (SELENOI) underlies a new disorder of Kennedy pathway phospholipid biosynthesis.

Observational study of gene-disease association. (HuGE Navigator)
Title: l-selectin gene polymorphisms and complications of sickle cell disease.

The following SELENOI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SELENOI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31317	NM_033505.4 Latest version!	Homo sapiens selenoprotein I (SELENOI), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$169.50-$237.30 $339.00
View Old Accession Versions >>
OHu31317	NM_033505.2	Homo sapiens ethanolaminephosphotransferase 1 (EPT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$169.50-$237.30 $339.00
OHu31317	NM_033505.3	Homo sapiens selenoprotein I (SELENOI), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$169.50-$237.30 $339.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SELENOI ( NM_033505.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_277040.1 Homo sapiens ethanolaminephosphotransferase 1 (EPT1), mRNA.

Price & Availability↑

CloneID	OHu31317
Clone ID Related Accession (Same CDS sequence)	NM_033505.2 , NM_033505.4 , NM_033505.3
Accession Version	NM_033505.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-09
Organism	Homo sapiens(Human)
Product	ethanolaminephosphotransferase 1
Comment

ATGGCTGGCT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTTTCTCTG TATGTCATGC ATCCATTCTG GAACACTATA 
GTAAAGGTAT TTCCTACTTG GCTGGCGCCC AATCTGATAA CTTTTTCTGG CTTTCTGCTG 
GTCGTATTCA ATTTTCTGCT AATGGCATAC TTTGATCCTG ACTTTTATGC CTCAGCACCA 
GGTCACAAGC ACGTGCCTGA CTGGGTTTGG ATTGTAGTGG GCATCCTCAA CTTCGTAGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCTCGCAGAA CCAATTCTAG CACTCCCTTA 
GGGGAGCTTT TTGATCATGG CCTGGATAGT TGGTCATGTG TTTACTTTGT TGTGACTGTT 
TATTCCATCT TTGGAAGAGG ATCAACTGGT GTCAGTGTTT TTGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCC CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCAGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATTAT TGGTTGTGCA TTATGTGTGA CTCTTCCAAT GAGTTTATTA 
AACTTTTTCA GAAGCTATAA AAATAACACC TTGAAACTCA ATTCAGTCTA TGAAGCTATG 
GTTCCCTTAT TTTCTCCATG CTTGCTGTTC ATTTTGTCTA CAGCGTGGAT CCTTTGGTCA 
CCTTCAGATA TTTTAGAGCT ACATCCTAGA GTATTCTACT TTATGGTTGG AACAGCTTTT 
GCCAACAGTA CATGTCAGCT GATTGTTTGC CAAATGAGTA GTACCCGGTG TCCAACTTTG 
AATTGGTTGC TGGTTCCTCT CTTCTTGGTT GTCTTAGTGG TAAACCTAGG AGTAGCCTCT 
TACGTTGAGA GCATTCTCCT GTATACATTA ACAACTGCTT TTACTCTGGC CCACATCCAT 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AGCCATTTTC AGATTTACCC CTTCTCATTG 
AGGAAACCAA ACTCAGATTG ACTAGGAATG GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277040.1
Translation	MAGYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSTGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAFANSTCQLIVCQMSSTRCPTLNWLLVPLFLVVLVVNLGVASYVESILLYTLTTAFTLAHIHYGVRVVKQLSSHFQIYPFSLRKPNSD*LGMEEKNIGL

Target ORF information:

RefSeq Version	NM_033505.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens ethanolaminephosphotransferase 1 (EPT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033505.2

ORF Insert Sequence:

ATGGCTGGCT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTTTCTCTG TATGTCATGC ATCCATTCTG GAACACTATA 
GTAAAGGTAT TTCCTACTTG GCTGGCGCCC AATCTGATAA CTTTTTCTGG CTTTCTGCTG 
GTCGTATTCA ATTTTCTGCT AATGGCATAC TTTGATCCTG ACTTTTATGC CTCAGCACCA 
GGTCACAAGC ACGTGCCTGA CTGGGTTTGG ATTGTAGTGG GCATCCTCAA CTTCGTAGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCTCGCAGAA CCAATTCTAG CACTCCCTTA 
GGGGAGCTTT TTGATCATGG CCTGGATAGT TGGTCATGTG TTTACTTTGT TGTGACTGTT 
TATTCCATCT TTGGAAGAGG ATCAACTGGT GTCAGTGTTT TTGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCC CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCAGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATTAT TGGTTGTGCA TTATGTGTGA CTCTTCCAAT GAGTTTATTA 
AACTTTTTCA GAAGCTATAA AAATAACACC TTGAAACTCA ATTCAGTCTA TGAAGCTATG 
GTTCCCTTAT TTTCTCCATG CTTGCTGTTC ATTTTGTCTA CAGCGTGGAT CCTTTGGTCA 
CCTTCAGATA TTTTAGAGCT ACATCCTAGA GTATTCTACT TTATGGTTGG AACAGCTTTT 
GCCAACAGTA CATGTCAGCT GATTGTTTGC CAAATGAGTA GTACCCGGTG TCCAACTTTG 
AATTGGTTGC TGGTTCCTCT CTTCTTGGTT GTCTTAGTGG TAAACCTAGG AGTAGCCTCT 
TACGTTGAGA GCATTCTCCT GTATACATTA ACAACTGCTT TTACTCTGGC CCACATCCAT 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AGCCATTTTC AGATTTACCC CTTCTCATTG 
AGGAAACCAA ACTCAGATTG ACTAGGAATG GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SELENOI ( NM_033505.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_277040.1 Homo sapiens selenoprotein I (SELENOI), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31317
Clone ID Related Accession (Same CDS sequence)	NM_033505.2 , NM_033505.4 , NM_033505.3
Accession Version	NM_033505.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Homo sapiens(human)
Product	ethanolaminephosphotransferase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010896.15. On Nov 23, 2018 this sequence version replaced NM_033505.3. Transcript Variant: This variant (1) represents the selenoprotein-encoding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BK001426.1, AB051511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260585.12/ ENSP00000260585.7 protein contains selenocysteine :: PMID: 12775843 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGGCT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTTTCTCTG TATGTCATGC ATCCATTCTG GAACACTATA 
GTAAAGGTAT TTCCTACTTG GCTGGCGCCC AATCTGATAA CTTTTTCTGG CTTTCTGCTG 
GTCGTATTCA ATTTTCTGCT AATGGCATAC TTTGATCCTG ACTTTTATGC CTCAGCACCA 
GGTCACAAGC ACGTGCCTGA CTGGGTTTGG ATTGTAGTGG GCATCCTCAA CTTCGTAGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCTCGCAGAA CCAATTCTAG CACTCCCTTA 
GGGGAGCTTT TTGATCATGG CCTGGATAGT TGGTCATGTG TTTACTTTGT TGTGACTGTT 
TATTCCATCT TTGGAAGAGG ATCAACTGGT GTCAGTGTTT TTGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCC CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCAGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATTAT TGGTTGTGCA TTATGTGTGA CTCTTCCAAT GAGTTTATTA 
AACTTTTTCA GAAGCTATAA AAATAACACC TTGAAACTCA ATTCAGTCTA TGAAGCTATG 
GTTCCCTTAT TTTCTCCATG CTTGCTGTTC ATTTTGTCTA CAGCGTGGAT CCTTTGGTCA 
CCTTCAGATA TTTTAGAGCT ACATCCTAGA GTATTCTACT TTATGGTTGG AACAGCTTTT 
GCCAACAGTA CATGTCAGCT GATTGTTTGC CAAATGAGTA GTACCCGGTG TCCAACTTTG 
AATTGGTTGC TGGTTCCTCT CTTCTTGGTT GTCTTAGTGG TAAACCTAGG AGTAGCCTCT 
TACGTTGAGA GCATTCTCCT GTATACATTA ACAACTGCTT TTACTCTGGC CCACATCCAT 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AGCCATTTTC AGATTTACCC CTTCTCATTG 
AGGAAACCAA ACTCAGATTG ACTAGGAATG GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277040.1
CDS	91..1284
Translation	MAGYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSTGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAFANSTCQLIVCQMSSTRCPTLNWLLVPLFLVVLVVNLGVASYVESILLYTLTTAFTLAHIHYGVRVVKQLSSHFQIYPFSLRKPNSD*LGMEEKNIGL

Target ORF information:

RefSeq Version	NM_033505.4
Organism	Homo sapiens(human)
Definition	Homo sapiens selenoprotein I (SELENOI), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033505.4

ORF Insert Sequence:

ATGGCTGGCT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTTTCTCTG TATGTCATGC ATCCATTCTG GAACACTATA 
GTAAAGGTAT TTCCTACTTG GCTGGCGCCC AATCTGATAA CTTTTTCTGG CTTTCTGCTG 
GTCGTATTCA ATTTTCTGCT AATGGCATAC TTTGATCCTG ACTTTTATGC CTCAGCACCA 
GGTCACAAGC ACGTGCCTGA CTGGGTTTGG ATTGTAGTGG GCATCCTCAA CTTCGTAGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCTCGCAGAA CCAATTCTAG CACTCCCTTA 
GGGGAGCTTT TTGATCATGG CCTGGATAGT TGGTCATGTG TTTACTTTGT TGTGACTGTT 
TATTCCATCT TTGGAAGAGG ATCAACTGGT GTCAGTGTTT TTGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCC CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCAGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATTAT TGGTTGTGCA TTATGTGTGA CTCTTCCAAT GAGTTTATTA 
AACTTTTTCA GAAGCTATAA AAATAACACC TTGAAACTCA ATTCAGTCTA TGAAGCTATG 
GTTCCCTTAT TTTCTCCATG CTTGCTGTTC ATTTTGTCTA CAGCGTGGAT CCTTTGGTCA 
CCTTCAGATA TTTTAGAGCT ACATCCTAGA GTATTCTACT TTATGGTTGG AACAGCTTTT 
GCCAACAGTA CATGTCAGCT GATTGTTTGC CAAATGAGTA GTACCCGGTG TCCAACTTTG 
AATTGGTTGC TGGTTCCTCT CTTCTTGGTT GTCTTAGTGG TAAACCTAGG AGTAGCCTCT 
TACGTTGAGA GCATTCTCCT GTATACATTA ACAACTGCTT TTACTCTGGC CCACATCCAT 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AGCCATTTTC AGATTTACCC CTTCTCATTG 
AGGAAACCAA ACTCAGATTG ACTAGGAATG GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SELENOI ( NM_033505.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_277040.1 Homo sapiens selenoprotein I (SELENOI), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31317
Clone ID Related Accession (Same CDS sequence)	NM_033505.2 , NM_033505.4 , NM_033505.3
Accession Version	NM_033505.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-19
Organism	Homo sapiens(human)
Product	ethanolaminephosphotransferase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA619246.1, AK095620.1, AC010896.15, AB051511.1, EH350337.1, AK092710.1, BU608897.1, DA235509.1, CF121730.1, CX758229.1, EH307484.1 and BE963795.2. On Jul 5, 2016 this sequence version replaced NM_033505.2. Transcript Variant: This variant (1) represents the protein-coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BK001426.1, AB051511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 12775843 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGCT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTTTCTCTG TATGTCATGC ATCCATTCTG GAACACTATA 
GTAAAGGTAT TTCCTACTTG GCTGGCGCCC AATCTGATAA CTTTTTCTGG CTTTCTGCTG 
GTCGTATTCA ATTTTCTGCT AATGGCATAC TTTGATCCTG ACTTTTATGC CTCAGCACCA 
GGTCACAAGC ACGTGCCTGA CTGGGTTTGG ATTGTAGTGG GCATCCTCAA CTTCGTAGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCTCGCAGAA CCAATTCTAG CACTCCCTTA 
GGGGAGCTTT TTGATCATGG CCTGGATAGT TGGTCATGTG TTTACTTTGT TGTGACTGTT 
TATTCCATCT TTGGAAGAGG ATCAACTGGT GTCAGTGTTT TTGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCC CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCAGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATTAT TGGTTGTGCA TTATGTGTGA CTCTTCCAAT GAGTTTATTA 
AACTTTTTCA GAAGCTATAA AAATAACACC TTGAAACTCA ATTCAGTCTA TGAAGCTATG 
GTTCCCTTAT TTTCTCCATG CTTGCTGTTC ATTTTGTCTA CAGCGTGGAT CCTTTGGTCA 
CCTTCAGATA TTTTAGAGCT ACATCCTAGA GTATTCTACT TTATGGTTGG AACAGCTTTT 
GCCAACAGTA CATGTCAGCT GATTGTTTGC CAAATGAGTA GTACCCGGTG TCCAACTTTG 
AATTGGTTGC TGGTTCCTCT CTTCTTGGTT GTCTTAGTGG TAAACCTAGG AGTAGCCTCT 
TACGTTGAGA GCATTCTCCT GTATACATTA ACAACTGCTT TTACTCTGGC CCACATCCAT 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AGCCATTTTC AGATTTACCC CTTCTCATTG 
AGGAAACCAA ACTCAGATTG ACTAGGAATG GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277040.1
CDS	148..1341
Translation	MAGYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSTGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAFANSTCQLIVCQMSSTRCPTLNWLLVPLFLVVLVVNLGVASYVESILLYTLTTAFTLAHIHYGVRVVKQLSSHFQIYPFSLRKPNSD*LGMEEKNIGL

Target ORF information:

RefSeq Version	NM_033505.3
Organism	Homo sapiens(human)
Definition	Homo sapiens selenoprotein I (SELENOI), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033505.3

ORF Insert Sequence:

ATGGCTGGCT ACGAATACGT GAGCCCGGAG CAGCTGGCTG GCTTTGATAA GTACAAGTAC 
AGTGCTGTGG ATACCAATCC ACTTTCTCTG TATGTCATGC ATCCATTCTG GAACACTATA 
GTAAAGGTAT TTCCTACTTG GCTGGCGCCC AATCTGATAA CTTTTTCTGG CTTTCTGCTG 
GTCGTATTCA ATTTTCTGCT AATGGCATAC TTTGATCCTG ACTTTTATGC CTCAGCACCA 
GGTCACAAGC ACGTGCCTGA CTGGGTTTGG ATTGTAGTGG GCATCCTCAA CTTCGTAGCC 
TACACTCTAG ATGGTGTGGA CGGAAAGCAA GCTCGCAGAA CCAATTCTAG CACTCCCTTA 
GGGGAGCTTT TTGATCATGG CCTGGATAGT TGGTCATGTG TTTACTTTGT TGTGACTGTT 
TATTCCATCT TTGGAAGAGG ATCAACTGGT GTCAGTGTTT TTGTTCTTTA TCTCCTGCTA 
TGGGTAGTTT TGTTTTCTTT CATCCTGTCC CACTGGGAAA AGTATAACAC AGGGATTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAG GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCAGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATTAT TGGTTGTGCA TTATGTGTGA CTCTTCCAAT GAGTTTATTA 
AACTTTTTCA GAAGCTATAA AAATAACACC TTGAAACTCA ATTCAGTCTA TGAAGCTATG 
GTTCCCTTAT TTTCTCCATG CTTGCTGTTC ATTTTGTCTA CAGCGTGGAT CCTTTGGTCA 
CCTTCAGATA TTTTAGAGCT ACATCCTAGA GTATTCTACT TTATGGTTGG AACAGCTTTT 
GCCAACAGTA CATGTCAGCT GATTGTTTGC CAAATGAGTA GTACCCGGTG TCCAACTTTG 
AATTGGTTGC TGGTTCCTCT CTTCTTGGTT GTCTTAGTGG TAAACCTAGG AGTAGCCTCT 
TACGTTGAGA GCATTCTCCT GTATACATTA ACAACTGCTT TTACTCTGGC CCACATCCAT 
TATGGAGTAC GAGTGGTAAA GCAGCTGAGC AGCCATTTTC AGATTTACCC CTTCTCATTG 
AGGAAACCAA ACTCAGATTG ACTAGGAATG GAAGAAAAGA ATATTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cloning, intracellular localization, and expression of the mammalian selenocysteine-containing protein SELENOI (SelI) in tumor cell lines.
Doklady. Biochemistry and biophysics476(1)320-322(2017 Sep)
Varlamova EG,Goltyaev MV,Novoselov VI,Fesenko EE

A mutation of EPT1 (SELENOI) underlies a new disorder of Kennedy pathway phospholipid biosynthesis.
Brain : a journal of neurology140(3)547-554(2017 Mar)
Ahmed MY,Al-Khayat A,Al-Murshedi F,Al-Futaisi A,Chioza BA,Pedro Fernandez-Murray J,Self JE,Salter CG,Harlalka GV,Rawlins LE,Al-Zuhaibi S,Al-Azri F,Al-Rashdi F,Cazenave-Gassiot A,Wenk MR,Al-Salmi F,Patton MA,Silver DL,Baple EL,McMaster CR,Crosby AH

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Investigative ophthalmology & visual science52(9)6662-70(2011 Aug)
Oshikawa M,Tsutsui C,Ikegami T,Fuchida Y,Matsubara M,Toyama S,Usami R,Ohtoko K,Kato S

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SELENOI cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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