PPFIA3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPFIA3
Entrez Gene ID	8541
Full Name	PTPRF interacting protein alpha 3
Synonyms	LPNA3
General protein information	Preferred Names PTPRF interacting protein alpha 3 Names liprin-alpha-3 liprin protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 protein tyrosine phosphatase, receptor type, f polypeptide, alpha 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin family protein has been shown to localize phosphatase LAR to cell focal adhesions and may be involved in the molecular organization of presynaptic active zones. [provided by RefSeq, Jul 2008].
Disorder MIM:	603144

mRNA and Protein(s)

mRNA	Protein	Name
XM_017027407.1	XP_016882896.1	liprin-alpha-3 isoform X1
NM_003660.3	NP_003651.1	liprin-alpha-3
NM_003660.4	NP_003651.1	liprin-alpha-3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PPFIA3	XP_005219545.1
Pan troglodytes (chimpanzee)	PPFIA3	XP_513009.3
Homo sapiens (human)	PPFIA3	NP_003651.1
Mus musculus (house mouse)	Ppfia3	NP_084017.2
Danio rerio (zebrafish)	ppfia3	XP_005164030.1
Xenopus tropicalis (tropical clawed frog)	ppfia3	XP_002934675.2
Canis lupus familiaris (dog)	PPFIA3	XP_005616919.1
Rattus norvegicus (Norway rat)	Ppfia3	NP_001257914.1

Gene Ontology
Bibliography

Related articles in PubMed

Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer.
Li WH, Zhou ZJ, Huang TH, Guo K, Chen W, Wang Y, Zhang H, Song YC, Chang DM
Disease markers20165780538(2016)

Liprin ?3: a putative estrogen regulated acrosomal protein.
Joshi CS, Suryawanshi AR, Khan SA, Balasinor NH, Khole VV
Histochemistry and cell biology139(4)535-48(2013 Apr)

Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening.
Wu C, Ma MH, Brown KR, Geisler M, Li L, Tzeng E, Jia CY, Jurisica I, Li SS
Proteomics7(11)1775-85(2007 Jun)

The synaptic vesicle cycle.
Sudhof TC
Annual review of neuroscience27509-47(2004)

Interaction of the ERC family of RIM-binding proteins with the liprin-alpha family of multidomain proteins.
Ko J, Na M, Kim S, Lee JR, Kim E
The Journal of biological chemistry278(43)42377-85(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results found that OSR2, VAV3, and PPFIA3 were significantly hypermethylated in gastric cancer (GC) patients offering a good alternative in a simple, promising, and noninvasive detection of GC.
Title: Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer.

The following PPFIA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPFIA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03455	NM_003660.4 Latest version!	Homo sapiens PTPRF interacting protein alpha 3 (PPFIA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu52559	XM_017027407.1 Latest version!	Homo sapiens PTPRF interacting protein alpha 3 (PPFIA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu03455	NM_003660.3	Homo sapiens PTPRF interacting protein alpha 3 (PPFIA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPFIA3 ( NM_003660.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003651.1 Homo sapiens PTPRF interacting protein alpha 3 (PPFIA3), transcript variant 1, mRNA.

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CloneID	OHu03455
Clone ID Related Accession (Same CDS sequence)	NM_003660.3 , NM_003660.4
Accession Version	NM_003660.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	liprin-alpha-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA236900.1, AB014554.2, AI346749.1 and BX111585.1. On Jul 12, 2013 this sequence version replaced NM_003660.2. Transcript Variant: This variant (1) encodes a functional protein. ##Evidence-Data-START## Transcript exon combination :: AB014554.2, BC101518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTGCG AGGTGATGCC CACCATCAGC GAGGATGGCC GGCGGGGCTC GGCGCTGGGC 
CCGGACGAGG CGGGCGGGGA GCTGGAGCGC CTCATGGTCA CGATGCTCAC GGAGCGCGAG 
CGCCTGCTGG AGACGCTGCG CGAGGCACAG GACGGGTTGG CTACAGCGCA GCTGCGGCTG 
CGCGAGCTCG GCCACGAGAA GGACTCGCTG CAGCGCCAGC TCAGCATCGC GCTGCCCCAG 
GAGTTTGCAG CTCTGACGAA GGAGCTGAAC TTATGTCGGG AGCAGCTGCT GGAGAGGGAG 
GAAGAGATTG CAGAGCTGAA GGCGGAACGG AACAACACGC GGCTGCTCCT GGAACACCTG 
GAGTGCCTGG TGTCCAGGCA CGAGAGGTCA CTGCGCATGA CCGTGGTGAA GCGCCAGGCC 
CAGTCCCCGG GTGGGGTCTC CTCGGAGGTA GAAGTGCTCA AAGCTCTAAA GTCTCTCTTC 
GAGCACCACA AGGCCCTGGA TGAGAAGGTC CGGGAGCGGC TGCGGATGGC GCTGGAGCGC 
GTGGCAGTGC TCGAGGAGGA GCTGGAACTG AGCAATCAGG AGACTCTGAA CCTTCGAGAA 
CAGCTGTCTA GGCGGCGGTC AGGGCTGGAA GAGCCGGGCA AGGATGGGGA TGGGCAGACT 
CTTGCCAATG GCCTGGGTCC TGGCGGGGAT TCCAACCGGC GCACAGCAGA GCTGGAGGAG 
GCCCTGGAGC GGCAGCGCGC CGAGGTGTGC CAGCTGCGGG AGCGCCTGGC GGTGCTGTGC 
CGTCAGATGA GCCAGCTGGA GGAGGAGTTG GGCACCGCGC ACCGTGAGCT GGGCAAGGCA 
GAGGAAGCCA ACTCCAAGCT GCAGCGCGAC CTCAAGGAGG CGCTGGCGCA GCGGGAAGAT 
ATGGAGGAGC GGATTACAAC ACTGGAGAAG CGCTACCTGA GCGCCCAGCG GGAGGCCACG 
TCTCTGCACG ACGCCAACGA CAAACTGGAG AACGAGTTAG CTAGCAAGGA GTCGTTGTAT 
CGGCAGAGTG AAGAGAAGAG CCGTCAGCTG GCCGAGTGGT TGGACGACGC CAAGCAGAAG 
CTGCAGCAGA CGCTGCAGAA AGCGGAGACC TTGCCCGAGA TAGAGGCGCA GCTGGCGCAG 
CGCGTGGCGG CGCTCAACAA GGCCGAGGAA CGTCATGGGA ATTTTGAGGA GCGGCTTCGG 
CAGCTGGAGG CCCAGCTGGA AGAGAAGAAT CAAGAGCTGC AGCGGGCCCG GCAGCGGGAG 
AAGATGAACG ATGACCACAA TAAGCGGCTG TCCGAGACGG TGGACAAGCT GCTGAGCGAG 
TCCAACGAGC GCTTACAGCT TCACCTCAAG GAGCGCATGG GGGCGCTGGA GGAGAAGAAC 
TCCCTGAGCG AGGAGATAGC CAACATGAAG AAGCTTCAGG ATGAGTTGCT GCTAAACAAG 
GAGCAGCTCT TGGCCGAAAT GGAGCGGATG CAGATGGAGA TCGACCAGCT GCGGGGGAGG 
CCACCATCCT CCTACTCCAG GTCTCTCCCT GGCAGTGCCC TGGAGCTCCG TTACTCTCAG 
GCACCCACTT TACCTTCTGG TGCCCACCTG GATCCCTATG TGGCTGGCAG TGGTCGGGCA 
GGCAAGAGGG GCCGCTGGTC AGGGGTCAAG GAGGAGCCCT CCAAGGATTG GGAGCGGTCT 
GCCCCTGCGG GCTCCATACC ACCCCCATTC CCTGGGGAAC TGGACGGCTC CGATGAGGAG 
GAGGCAGAGG GGATGTTTGG GGCCGAGCTG CTGTCCCCCA GTGGGCAGGC TGACGTGCAG 
ACGCTGGCCA TCATGCTTCA GGAGCAGCTG GAGGCCATCA ACAAGGAGAT CAAGCTGATC 
CAAGAGGAGA AGGAGACAAC AGAACAGAGG GCAGAGGAGC TGGAGAGTCG GGTGTCCAGC 
TCTGGCTTGG ACTCGTTGGG CCGCTACCGC AGCAGCTGCT CCCTGCCCCC CTCCCTCACC 
ACCTCTACCC TTGCCAGCCC CTCCCCTCCC AGCTCTGGCC ACTCAACACC CCGCCTGGCA 
CCCCCTAGCC CTGCCCGTGA GGGCACCGAC AAGGCTAATC ATGTCCCTAA GGAGGAAGCT 
GGAGCTCCAC GAGGGGAGGG GCCGGCCATC CCAGGAGACA CCCCACCACC CACTCCCCGC 
TCTGCCCGTC TTGAGAGAAT GACCCAGGCC TTGGCACTGC AGGCGGGGTC CCTGGAAGAT 
GGGGGACCCC CACGGGGAAG TGAGGGCACC CCAGATTCTC TGCACAAAGC CCCCAAGAAG 
AAGAGCATCA AGTCATCCAT AGGCCGTCTC TTTGGCAAGA AAGAGAAGGG ACGAATGGGA 
CCCCCAGGCC GGGACAGCTC TTCTCTGGCT GGAACACCCT CAGATGAGAC ACTGGCCACT 
GACCCTCTGG GGCTAGCCAA GCTGACAGGC CCAGGAGACA AGGACCGAAG GAACAAGAGG 
AAGCATGAAC TCCTGGAGGA GGCCTGCCGC CAGGGCCTAC CTTTTGCTGC CTGGGACGGG 
CCCACCGTGG TGTCCTGGCT GGAGCTGTGG GTGGGCATGC CTGCCTGGTA TGTGGCCGCC 
TGCCGGGCCA ATGTCAAGAG CGGTGCCATC ATGGCCAACC TGTCAGACAC GGAGATCCAG 
CGCGAGATCG GCATCAGCAA CCCGCTGCAC CGACTCAAGC TACGCCTCGC CATCCAGGAG 
ATGGTCTCGC TCACCTCGCC CTCAGCCCCC GCCTCCTCCC GCACTTCCAC AGGAAACGTG 
TGGATGACAC ACGAGGAGAT GGAGTCCCTT ACGGCCACGA CCAAGCCCGA GACCAAGGAG 
ATCAGCTGGG AGCAGATCCT GGCATATGGC GACATGAACC ACGAGTGGGT GGGGAACGAC 
TGGCTGCCCA GCCTGGGGCT GCCCCAATAC CGCAGCTACT TCATGGAGTC GCTGGTGGAC 
GCTCGAATGT TAGATCACCT TAACAAGAAG GAGCTCCGGG GCCAACTCAA GATGGTGGAC 
AGCTTTCACA GGGTGAGTCT ACATTATGGG ATTATGTGCC TGAAACGGCT CAACTATGAC 
CGGAAGGACC TGGAGCGGAG GCGGGAAGAA AGTCAGACCC AGATCCGAGA CGTGATGGTG 
TGGTCCAATG AGCGGGTCAT GGGTTGGGTG TCCGGGCTGG GCCTGAAGGA ATTTGCCACG 
AACCTCACGG AGAGCGGGGT ACACGGGGCA CTGCTCGCCC TGGACGAGAC CTTCGACTAC 
TCCGACCTGG CCTTGCTCCT GCAGATCCCC ACGCAGAATG CACAGGCCCG GCAGCTTCTG 
GAGAAGGAAT TCAGCAACCT TATCTCCTTA GGCACAGACA GGCGGCTGGA CGAGGACAGC 
GCCAAGTCTT TCAGCCGCTC CCCATCCTGG CGGAAGATGT TCCGGGAGAA GGACCTCCGA 
GGCGTAACTC CCGACTCAGC TGAGATGTTG CCCCCCAACT TTCGTTCGGC TGCAGCGGGA 
GCCCTGGGCT CTCCGGGGCT CCCTCTCCGC AAGCTGCAGC CAGAAGGCCA GACTTCTGGG 
AGTTCCCGGG CAGACGGCGT TTCGGTCCGG ACCTATTCCT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003651.1
CDS	350..3934
Translation	MMCEVMPTISEDGRRGSALGPDEAGGELERLMVTMLTERERLLETLREAQDGLATAQLRLRELGHEKDSLQRQLSIALPQEFAALTKELNLCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPGGVSSEVEVLKALKSLFEHHKALDEKVRERLRMALERVAVLEEELELSNQETLNLREQLSRRRSGLEEPGKDGDGQTLANGLGPGGDSNRRTAELEEALERQRAEVCQLRERLAVLCRQMSQLEEELGTAHRELGKAEEANSKLQRDLKEALAQREDMEERITTLEKRYLSAQREATSLHDANDKLENELASKESLYRQSEEKSRQLAEWLDDAKQKLQQTLQKAETLPEIEAQLAQRVAALNKAEERHGNFEERLRQLEAQLEEKNQELQRARQREKMNDDHNKRLSETVDKLLSESNERLQLHLKERMGALEEKNSLSEEIANMKKLQDELLLNKEQLLAEMERMQMEIDQLRGRPPSSYSRSLPGSALELRYSQAPTLPSGAHLDPYVAGSGRAGKRGRWSGVKEEPSKDWERSAPAGSIPPPFPGELDGSDEEEAEGMFGAELLSPSGQADVQTLAIMLQEQLEAINKEIKLIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKANHVPKEEAGAPRGEGPAIPGDTPPPTPRSARLERMTQALALQAGSLEDGGPPRGSEGTPDSLHKAPKKKSIKSSIGRLFGKKEKGRMGPPGRDSSSLAGTPSDETLATDPLGLAKLTGPGDKDRRNKRKHELLEEACRQGLPFAAWDGPTVVSWLELWVGMPAWYVAACRANVKSGAIMANLSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPASSRTSTGNVWMTHEEMESLTATTKPETKEISWEQILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLNKKELRGQLKMVDSFHRVSLHYGIMCLKRLNYDRKDLERRREESQTQIRDVMVWSNERVMGWVSGLGLKEFATNLTESGVHGALLALDETFDYSDLALLLQIPTQNAQARQLLEKEFSNLISLGTDRRLDEDSAKSFSRSPSWRKMFREKDLRGVTPDSAEMLPPNFRSAAAGALGSPGLPLRKLQPEGQTSGSSRADGVSVRTYSC

Target ORF information:

RefSeq Version	NM_003660.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 3 (PPFIA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003660.3

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC CACCATCAGC GAGGATGGCC GGCGGGGCTC GGCGCTGGGC 
CCGGACGAGG CGGGCGGGGA GCTGGAGCGC CTCATGGTCA CGATGCTCAC GGAGCGCGAG 
CGCCTGCTGG AGACGCTGCG CGAGGCACAG GACGGGTTGG CTACAGCGCA GCTGCGGCTG 
CGCGAGCTCG GCCACGAGAA GGACTCGCTG CAGCGCCAGC TCAGCATCGC GCTGCCCCAG 
GAGTTTGCAG CTCTGACGAA GGAGCTGAAC TTATGTCGGG AGCAGCTGCT GGAGAGGGAG 
GAAGAGATTG CAGAGCTGAA GGCGGAACGG AACAACACGC GGCTGCTCCT GGAACACCTG 
GAGTGCCTGG TGTCCAGGCA CGAGAGGTCA CTGCGCATGA CCGTGGTGAA GCGCCAGGCC 
CAGTCCCCGG GTGGGGTCTC CTCGGAGGTA GAAGTGCTCA AAGCTCTAAA GTCTCTCTTC 
GAGCACCACA AGGCCCTGGA TGAGAAGGTC CGGGAGCGGC TGCGGATGGC GCTGGAGCGC 
GTGGCAGTGC TCGAGGAGGA GCTGGAACTG AGCAATCAGG AGACTCTGAA CCTTCGAGAA 
CAGCTGTCTA GGCGGCGGTC AGGGCTGGAA GAGCCGGGCA AGGATGGGGA TGGGCAGACT 
CTTGCCAATG GCCTGGGTCC TGGCGGGGAT TCCAACCGGC GCACAGCAGA GCTGGAGGAG 
GCCCTGGAGC GGCAGCGCGC CGAGGTGTGC CAGCTGCGGG AGCGCCTGGC GGTGCTGTGC 
CGTCAGATGA GCCAGCTGGA GGAGGAGTTG GGCACCGCGC ACCGTGAGCT GGGCAAGGCA 
GAGGAAGCCA ACTCCAAGCT GCAGCGCGAC CTCAAGGAGG CGCTGGCGCA GCGGGAAGAT 
ATGGAGGAGC GGATTACAAC ACTGGAGAAG CGCTACCTGA GCGCCCAGCG GGAGGCCACG 
TCTCTGCACG ACGCCAACGA CAAACTGGAG AACGAGTTAG CTAGCAAGGA GTCGTTGTAT 
CGGCAGAGTG AAGAGAAGAG CCGTCAGCTG GCCGAGTGGT TGGACGACGC CAAGCAGAAG 
CTGCAGCAGA CGCTGCAGAA AGCGGAGACC TTGCCCGAGA TAGAGGCGCA GCTGGCGCAG 
CGCGTGGCGG CGCTCAACAA GGCCGAGGAA CGTCATGGGA ATTTTGAGGA GCGGCTTCGG 
CAGCTGGAGG CCCAGCTGGA AGAGAAGAAT CAAGAGCTGC AGCGGGCCCG GCAGCGGGAG 
AAGATGAACG ATGACCACAA TAAGCGGCTG TCCGAGACGG TGGACAAGCT GCTGAGCGAG 
TCCAACGAGC GCTTACAGCT TCACCTCAAG GAGCGCATGG GGGCGCTGGA GGAGAAGAAC 
TCCCTGAGCG AGGAGATAGC CAACATGAAG AAGCTTCAGG ATGAGTTGCT GCTAAACAAG 
GAGCAGCTCT TGGCCGAAAT GGAGCGGATG CAGATGGAGA TCGACCAGCT GCGGGGGAGG 
CCACCATCCT CCTACTCCAG GTCTCTCCCT GGCAGTGCCC TGGAGCTCCG TTACTCTCAG 
GCACCCACTT TACCTTCTGG TGCCCACCTG GATCCCTATG TGGCTGGCAG TGGTCGGGCA 
GGCAAGAGGG GCCGCTGGTC AGGGGTCAAG GAGGAGCCCT CCAAGGATTG GGAGCGGTCT 
GCCCCTGCGG GCTCCATACC ACCCCCATTC CCTGGGGAAC TGGACGGCTC CGATGAGGAG 
GAGGCAGAGG GGATGTTTGG GGCCGAGCTG CTGTCCCCCA GTGGGCAGGC TGACGTGCAG 
ACGCTGGCCA TCATGCTTCA GGAGCAGCTG GAGGCCATCA ACAAGGAGAT CAAGCTGATC 
CAAGAGGAGA AGGAGACAAC AGAACAGAGG GCAGAGGAGC TGGAGAGTCG GGTGTCCAGC 
TCTGGCTTGG ACTCGTTGGG CCGCTACCGC AGCAGCTGCT CCCTGCCCCC CTCCCTCACC 
ACCTCTACCC TTGCCAGCCC CTCCCCTCCC AGCTCTGGCC ACTCAACACC CCGCCTGGCA 
CCCCCTAGCC CTGCCCGTGA GGGCACCGAC AAGGCTAATC ATGTCCCTAA GGAGGAAGCT 
GGAGCTCCAC GAGGGGAGGG GCCGGCCATC CCAGGAGACA CCCCACCACC CACTCCCCGC 
TCTGCCCGTC TTGAGAGAAT GACCCAGGCC TTGGCACTGC AGGCGGGGTC CCTGGAAGAT 
GGGGGACCCC CACGGGGAAG TGAGGGCACC CCAGATTCTC TGCACAAAGC CCCCAAGAAG 
AAGAGCATCA AGTCATCCAT AGGCCGTCTC TTTGGCAAGA AAGAGAAGGG ACGAATGGGA 
CCCCCAGGCC GGGACAGCTC TTCTCTGGCT GGAACACCCT CAGATGAGAC ACTGGCCACT 
GACCCTCTGG GGCTAGCCAA GCTGACAGGC CCAGGAGACA AGGACCGAAG GAACAAGAGG 
AAGCATGAAC TCCTGGAGGA GGCCTGCCGC CAGGGCCTAC CTTTTGCTGC CTGGGACGGG 
CCCACCGTGG TGTCCTGGCT GGAGCTGTGG GTGGGCATGC CTGCCTGGTA TGTGGCCGCC 
TGCCGGGCCA ATGTCAAGAG CGGTGCCATC ATGGCCAACC TGTCAGACAC GGAGATCCAG 
CGCGAGATCG GCATCAGCAA CCCGCTGCAC CGACTCAAGC TACGCCTCGC CATCCAGGAG 
ATGGTCTCGC TCACCTCGCC CTCAGCCCCC GCCTCCTCCC GCACTTCCAC AGGAAACGTG 
TGGATGACAC ACGAGGAGAT GGAGTCCCTT ACGGCCACGA CCAAGCCCGA GACCAAGGAG 
ATCAGCTGGG AGCAGATCCT GGCATATGGC GACATGAACC ACGAGTGGGT GGGGAACGAC 
TGGCTGCCCA GCCTGGGGCT GCCCCAATAC CGCAGCTACT TCATGGAGTC GCTGGTGGAC 
GCTCGAATGT TAGATCACCT TAACAAGAAG GAGCTCCGGG GCCAACTCAA GATGGTGGAC 
AGCTTTCACA GGGTGAGTCT ACATTATGGG ATTATGTGCC TGAAACGGCT CAACTATGAC 
CGGAAGGACC TGGAGCGGAG GCGGGAAGAA AGTCAGACCC AGATCCGAGA CGTGATGGTG 
TGGTCCAATG AGCGGGTCAT GGGTTGGGTG TCCGGGCTGG GCCTGAAGGA ATTTGCCACG 
AACCTCACGG AGAGCGGGGT ACACGGGGCA CTGCTCGCCC TGGACGAGAC CTTCGACTAC 
TCCGACCTGG CCTTGCTCCT GCAGATCCCC ACGCAGAATG CACAGGCCCG GCAGCTTCTG 
GAGAAGGAAT TCAGCAACCT TATCTCCTTA GGCACAGACA GGCGGCTGGA CGAGGACAGC 
GCCAAGTCTT TCAGCCGCTC CCCATCCTGG CGGAAGATGT TCCGGGAGAA GGACCTCCGA 
GGCGTAACTC CCGACTCAGC TGAGATGTTG CCCCCCAACT TTCGTTCGGC TGCAGCGGGA 
GCCCTGGGCT CTCCGGGGCT CCCTCTCCGC AAGCTGCAGC CAGAAGGCCA GACTTCTGGG 
AGTTCCCGGG CAGACGGCGT TTCGGTCCGG ACCTATTCCT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA3 ( NM_003660.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003651.1 Homo sapiens PTPRF interacting protein alpha 3 (PPFIA3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03455
Clone ID Related Accession (Same CDS sequence)	NM_003660.3 , NM_003660.4
Accession Version	NM_003660.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	liprin-alpha-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA236900.1, AB014554.2, AI346749.1 and BX111585.1. On Nov 23, 2018 this sequence version replaced NM_003660.3. Transcript Variant: This variant (1) encodes a functional protein. ##Evidence-Data-START## Transcript exon combination :: AB014554.2, BC101518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000334186.9/ ENSP00000335614.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGTGCG AGGTGATGCC CACCATCAGC GAGGATGGCC GGCGGGGCTC GGCGCTGGGC 
CCGGACGAGG CGGGCGGGGA GCTGGAGCGC CTCATGGTCA CGATGCTCAC GGAGCGCGAG 
CGCCTGCTGG AGACGCTGCG CGAGGCACAG GACGGGTTGG CTACAGCGCA GCTGCGGCTG 
CGCGAGCTCG GCCACGAGAA GGACTCGCTG CAGCGCCAGC TCAGCATCGC GCTGCCCCAG 
GAGTTTGCAG CTCTGACGAA GGAGCTGAAC TTATGTCGGG AGCAGCTGCT GGAGAGGGAG 
GAAGAGATTG CAGAGCTGAA GGCGGAACGG AACAACACGC GGCTGCTCCT GGAACACCTG 
GAGTGCCTGG TGTCCAGGCA CGAGAGGTCA CTGCGCATGA CCGTGGTGAA GCGCCAGGCC 
CAGTCCCCGG GTGGGGTCTC CTCGGAGGTA GAAGTGCTCA AAGCTCTAAA GTCTCTCTTC 
GAGCACCACA AGGCCCTGGA TGAGAAGGTC CGGGAGCGGC TGCGGATGGC GCTGGAGCGC 
GTGGCAGTGC TCGAGGAGGA GCTGGAACTG AGCAATCAGG AGACTCTGAA CCTTCGAGAA 
CAGCTGTCTA GGCGGCGGTC AGGGCTGGAA GAGCCGGGCA AGGATGGGGA TGGGCAGACT 
CTTGCCAATG GCCTGGGTCC TGGCGGGGAT TCCAACCGGC GCACAGCAGA GCTGGAGGAG 
GCCCTGGAGC GGCAGCGCGC CGAGGTGTGC CAGCTGCGGG AGCGCCTGGC GGTGCTGTGC 
CGTCAGATGA GCCAGCTGGA GGAGGAGTTG GGCACCGCGC ACCGTGAGCT GGGCAAGGCA 
GAGGAAGCCA ACTCCAAGCT GCAGCGCGAC CTCAAGGAGG CGCTGGCGCA GCGGGAAGAT 
ATGGAGGAGC GGATTACAAC ACTGGAGAAG CGCTACCTGA GCGCCCAGCG GGAGGCCACG 
TCTCTGCACG ACGCCAACGA CAAACTGGAG AACGAGTTAG CTAGCAAGGA GTCGTTGTAT 
CGGCAGAGTG AAGAGAAGAG CCGTCAGCTG GCCGAGTGGT TGGACGACGC CAAGCAGAAG 
CTGCAGCAGA CGCTGCAGAA AGCGGAGACC TTGCCCGAGA TAGAGGCGCA GCTGGCGCAG 
CGCGTGGCGG CGCTCAACAA GGCCGAGGAA CGTCATGGGA ATTTTGAGGA GCGGCTTCGG 
CAGCTGGAGG CCCAGCTGGA AGAGAAGAAT CAAGAGCTGC AGCGGGCCCG GCAGCGGGAG 
AAGATGAACG ATGACCACAA TAAGCGGCTG TCCGAGACGG TGGACAAGCT GCTGAGCGAG 
TCCAACGAGC GCTTACAGCT TCACCTCAAG GAGCGCATGG GGGCGCTGGA GGAGAAGAAC 
TCCCTGAGCG AGGAGATAGC CAACATGAAG AAGCTTCAGG ATGAGTTGCT GCTAAACAAG 
GAGCAGCTCT TGGCCGAAAT GGAGCGGATG CAGATGGAGA TCGACCAGCT GCGGGGGAGG 
CCACCATCCT CCTACTCCAG GTCTCTCCCT GGCAGTGCCC TGGAGCTCCG TTACTCTCAG 
GCACCCACTT TACCTTCTGG TGCCCACCTG GATCCCTATG TGGCTGGCAG TGGTCGGGCA 
GGCAAGAGGG GCCGCTGGTC AGGGGTCAAG GAGGAGCCCT CCAAGGATTG GGAGCGGTCT 
GCCCCTGCGG GCTCCATACC ACCCCCATTC CCTGGGGAAC TGGACGGCTC CGATGAGGAG 
GAGGCAGAGG GGATGTTTGG GGCCGAGCTG CTGTCCCCCA GTGGGCAGGC TGACGTGCAG 
ACGCTGGCCA TCATGCTTCA GGAGCAGCTG GAGGCCATCA ACAAGGAGAT CAAGCTGATC 
CAAGAGGAGA AGGAGACAAC AGAACAGAGG GCAGAGGAGC TGGAGAGTCG GGTGTCCAGC 
TCTGGCTTGG ACTCGTTGGG CCGCTACCGC AGCAGCTGCT CCCTGCCCCC CTCCCTCACC 
ACCTCTACCC TTGCCAGCCC CTCCCCTCCC AGCTCTGGCC ACTCAACACC CCGCCTGGCA 
CCCCCTAGCC CTGCCCGTGA GGGCACCGAC AAGGCTAATC ATGTCCCTAA GGAGGAAGCT 
GGAGCTCCAC GAGGGGAGGG GCCGGCCATC CCAGGAGACA CCCCACCACC CACTCCCCGC 
TCTGCCCGTC TTGAGAGAAT GACCCAGGCC TTGGCACTGC AGGCGGGGTC CCTGGAAGAT 
GGGGGACCCC CACGGGGAAG TGAGGGCACC CCAGATTCTC TGCACAAAGC CCCCAAGAAG 
AAGAGCATCA AGTCATCCAT AGGCCGTCTC TTTGGCAAGA AAGAGAAGGG ACGAATGGGA 
CCCCCAGGCC GGGACAGCTC TTCTCTGGCT GGAACACCCT CAGATGAGAC ACTGGCCACT 
GACCCTCTGG GGCTAGCCAA GCTGACAGGC CCAGGAGACA AGGACCGAAG GAACAAGAGG 
AAGCATGAAC TCCTGGAGGA GGCCTGCCGC CAGGGCCTAC CTTTTGCTGC CTGGGACGGG 
CCCACCGTGG TGTCCTGGCT GGAGCTGTGG GTGGGCATGC CTGCCTGGTA TGTGGCCGCC 
TGCCGGGCCA ATGTCAAGAG CGGTGCCATC ATGGCCAACC TGTCAGACAC GGAGATCCAG 
CGCGAGATCG GCATCAGCAA CCCGCTGCAC CGACTCAAGC TACGCCTCGC CATCCAGGAG 
ATGGTCTCGC TCACCTCGCC CTCAGCCCCC GCCTCCTCCC GCACTTCCAC AGGAAACGTG 
TGGATGACAC ACGAGGAGAT GGAGTCCCTT ACGGCCACGA CCAAGCCCGA GACCAAGGAG 
ATCAGCTGGG AGCAGATCCT GGCATATGGC GACATGAACC ACGAGTGGGT GGGGAACGAC 
TGGCTGCCCA GCCTGGGGCT GCCCCAATAC CGCAGCTACT TCATGGAGTC GCTGGTGGAC 
GCTCGAATGT TAGATCACCT TAACAAGAAG GAGCTCCGGG GCCAACTCAA GATGGTGGAC 
AGCTTTCACA GGGTGAGTCT ACATTATGGG ATTATGTGCC TGAAACGGCT CAACTATGAC 
CGGAAGGACC TGGAGCGGAG GCGGGAAGAA AGTCAGACCC AGATCCGAGA CGTGATGGTG 
TGGTCCAATG AGCGGGTCAT GGGTTGGGTG TCCGGGCTGG GCCTGAAGGA ATTTGCCACG 
AACCTCACGG AGAGCGGGGT ACACGGGGCA CTGCTCGCCC TGGACGAGAC CTTCGACTAC 
TCCGACCTGG CCTTGCTCCT GCAGATCCCC ACGCAGAATG CACAGGCCCG GCAGCTTCTG 
GAGAAGGAAT TCAGCAACCT TATCTCCTTA GGCACAGACA GGCGGCTGGA CGAGGACAGC 
GCCAAGTCTT TCAGCCGCTC CCCATCCTGG CGGAAGATGT TCCGGGAGAA GGACCTCCGA 
GGCGTAACTC CCGACTCAGC TGAGATGTTG CCCCCCAACT TTCGTTCGGC TGCAGCGGGA 
GCCCTGGGCT CTCCGGGGCT CCCTCTCCGC AAGCTGCAGC CAGAAGGCCA GACTTCTGGG 
AGTTCCCGGG CAGACGGCGT TTCGGTCCGG ACCTATTCCT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003651.1
CDS	195..3779
Translation	MMCEVMPTISEDGRRGSALGPDEAGGELERLMVTMLTERERLLETLREAQDGLATAQLRLRELGHEKDSLQRQLSIALPQEFAALTKELNLCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPGGVSSEVEVLKALKSLFEHHKALDEKVRERLRMALERVAVLEEELELSNQETLNLREQLSRRRSGLEEPGKDGDGQTLANGLGPGGDSNRRTAELEEALERQRAEVCQLRERLAVLCRQMSQLEEELGTAHRELGKAEEANSKLQRDLKEALAQREDMEERITTLEKRYLSAQREATSLHDANDKLENELASKESLYRQSEEKSRQLAEWLDDAKQKLQQTLQKAETLPEIEAQLAQRVAALNKAEERHGNFEERLRQLEAQLEEKNQELQRARQREKMNDDHNKRLSETVDKLLSESNERLQLHLKERMGALEEKNSLSEEIANMKKLQDELLLNKEQLLAEMERMQMEIDQLRGRPPSSYSRSLPGSALELRYSQAPTLPSGAHLDPYVAGSGRAGKRGRWSGVKEEPSKDWERSAPAGSIPPPFPGELDGSDEEEAEGMFGAELLSPSGQADVQTLAIMLQEQLEAINKEIKLIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKANHVPKEEAGAPRGEGPAIPGDTPPPTPRSARLERMTQALALQAGSLEDGGPPRGSEGTPDSLHKAPKKKSIKSSIGRLFGKKEKGRMGPPGRDSSSLAGTPSDETLATDPLGLAKLTGPGDKDRRNKRKHELLEEACRQGLPFAAWDGPTVVSWLELWVGMPAWYVAACRANVKSGAIMANLSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPASSRTSTGNVWMTHEEMESLTATTKPETKEISWEQILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLNKKELRGQLKMVDSFHRVSLHYGIMCLKRLNYDRKDLERRREESQTQIRDVMVWSNERVMGWVSGLGLKEFATNLTESGVHGALLALDETFDYSDLALLLQIPTQNAQARQLLEKEFSNLISLGTDRRLDEDSAKSFSRSPSWRKMFREKDLRGVTPDSAEMLPPNFRSAAAGALGSPGLPLRKLQPEGQTSGSSRADGVSVRTYSC

Target ORF information:

RefSeq Version	NM_003660.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 3 (PPFIA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003660.4

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC CACCATCAGC GAGGATGGCC GGCGGGGCTC GGCGCTGGGC 
CCGGACGAGG CGGGCGGGGA GCTGGAGCGC CTCATGGTCA CGATGCTCAC GGAGCGCGAG 
CGCCTGCTGG AGACGCTGCG CGAGGCACAG GACGGGTTGG CTACAGCGCA GCTGCGGCTG 
CGCGAGCTCG GCCACGAGAA GGACTCGCTG CAGCGCCAGC TCAGCATCGC GCTGCCCCAG 
GAGTTTGCAG CTCTGACGAA GGAGCTGAAC TTATGTCGGG AGCAGCTGCT GGAGAGGGAG 
GAAGAGATTG CAGAGCTGAA GGCGGAACGG AACAACACGC GGCTGCTCCT GGAACACCTG 
GAGTGCCTGG TGTCCAGGCA CGAGAGGTCA CTGCGCATGA CCGTGGTGAA GCGCCAGGCC 
CAGTCCCCGG GTGGGGTCTC CTCGGAGGTA GAAGTGCTCA AAGCTCTAAA GTCTCTCTTC 
GAGCACCACA AGGCCCTGGA TGAGAAGGTC CGGGAGCGGC TGCGGATGGC GCTGGAGCGC 
GTGGCAGTGC TCGAGGAGGA GCTGGAACTG AGCAATCAGG AGACTCTGAA CCTTCGAGAA 
CAGCTGTCTA GGCGGCGGTC AGGGCTGGAA GAGCCGGGCA AGGATGGGGA TGGGCAGACT 
CTTGCCAATG GCCTGGGTCC TGGCGGGGAT TCCAACCGGC GCACAGCAGA GCTGGAGGAG 
GCCCTGGAGC GGCAGCGCGC CGAGGTGTGC CAGCTGCGGG AGCGCCTGGC GGTGCTGTGC 
CGTCAGATGA GCCAGCTGGA GGAGGAGTTG GGCACCGCGC ACCGTGAGCT GGGCAAGGCA 
GAGGAAGCCA ACTCCAAGCT GCAGCGCGAC CTCAAGGAGG CGCTGGCGCA GCGGGAAGAT 
ATGGAGGAGC GGATTACAAC ACTGGAGAAG CGCTACCTGA GCGCCCAGCG GGAGGCCACG 
TCTCTGCACG ACGCCAACGA CAAACTGGAG AACGAGTTAG CTAGCAAGGA GTCGTTGTAT 
CGGCAGAGTG AAGAGAAGAG CCGTCAGCTG GCCGAGTGGT TGGACGACGC CAAGCAGAAG 
CTGCAGCAGA CGCTGCAGAA AGCGGAGACC TTGCCCGAGA TAGAGGCGCA GCTGGCGCAG 
CGCGTGGCGG CGCTCAACAA GGCCGAGGAA CGTCATGGGA ATTTTGAGGA GCGGCTTCGG 
CAGCTGGAGG CCCAGCTGGA AGAGAAGAAT CAAGAGCTGC AGCGGGCCCG GCAGCGGGAG 
AAGATGAACG ATGACCACAA TAAGCGGCTG TCCGAGACGG TGGACAAGCT GCTGAGCGAG 
TCCAACGAGC GCTTACAGCT TCACCTCAAG GAGCGCATGG GGGCGCTGGA GGAGAAGAAC 
TCCCTGAGCG AGGAGATAGC CAACATGAAG AAGCTTCAGG ATGAGTTGCT GCTAAACAAG 
GAGCAGCTCT TGGCCGAAAT GGAGCGGATG CAGATGGAGA TCGACCAGCT GCGGGGGAGG 
CCACCATCCT CCTACTCCAG GTCTCTCCCT GGCAGTGCCC TGGAGCTCCG TTACTCTCAG 
GCACCCACTT TACCTTCTGG TGCCCACCTG GATCCCTATG TGGCTGGCAG TGGTCGGGCA 
GGCAAGAGGG GCCGCTGGTC AGGGGTCAAG GAGGAGCCCT CCAAGGATTG GGAGCGGTCT 
GCCCCTGCGG GCTCCATACC ACCCCCATTC CCTGGGGAAC TGGACGGCTC CGATGAGGAG 
GAGGCAGAGG GGATGTTTGG GGCCGAGCTG CTGTCCCCCA GTGGGCAGGC TGACGTGCAG 
ACGCTGGCCA TCATGCTTCA GGAGCAGCTG GAGGCCATCA ACAAGGAGAT CAAGCTGATC 
CAAGAGGAGA AGGAGACAAC AGAACAGAGG GCAGAGGAGC TGGAGAGTCG GGTGTCCAGC 
TCTGGCTTGG ACTCGTTGGG CCGCTACCGC AGCAGCTGCT CCCTGCCCCC CTCCCTCACC 
ACCTCTACCC TTGCCAGCCC CTCCCCTCCC AGCTCTGGCC ACTCAACACC CCGCCTGGCA 
CCCCCTAGCC CTGCCCGTGA GGGCACCGAC AAGGCTAATC ATGTCCCTAA GGAGGAAGCT 
GGAGCTCCAC GAGGGGAGGG GCCGGCCATC CCAGGAGACA CCCCACCACC CACTCCCCGC 
TCTGCCCGTC TTGAGAGAAT GACCCAGGCC TTGGCACTGC AGGCGGGGTC CCTGGAAGAT 
GGGGGACCCC CACGGGGAAG TGAGGGCACC CCAGATTCTC TGCACAAAGC CCCCAAGAAG 
AAGAGCATCA AGTCATCCAT AGGCCGTCTC TTTGGCAAGA AAGAGAAGGG ACGAATGGGA 
CCCCCAGGCC GGGACAGCTC TTCTCTGGCT GGAACACCCT CAGATGAGAC ACTGGCCACT 
GACCCTCTGG GGCTAGCCAA GCTGACAGGC CCAGGAGACA AGGACCGAAG GAACAAGAGG 
AAGCATGAAC TCCTGGAGGA GGCCTGCCGC CAGGGCCTAC CTTTTGCTGC CTGGGACGGG 
CCCACCGTGG TGTCCTGGCT GGAGCTGTGG GTGGGCATGC CTGCCTGGTA TGTGGCCGCC 
TGCCGGGCCA ATGTCAAGAG CGGTGCCATC ATGGCCAACC TGTCAGACAC GGAGATCCAG 
CGCGAGATCG GCATCAGCAA CCCGCTGCAC CGACTCAAGC TACGCCTCGC CATCCAGGAG 
ATGGTCTCGC TCACCTCGCC CTCAGCCCCC GCCTCCTCCC GCACTTCCAC AGGAAACGTG 
TGGATGACAC ACGAGGAGAT GGAGTCCCTT ACGGCCACGA CCAAGCCCGA GACCAAGGAG 
ATCAGCTGGG AGCAGATCCT GGCATATGGC GACATGAACC ACGAGTGGGT GGGGAACGAC 
TGGCTGCCCA GCCTGGGGCT GCCCCAATAC CGCAGCTACT TCATGGAGTC GCTGGTGGAC 
GCTCGAATGT TAGATCACCT TAACAAGAAG GAGCTCCGGG GCCAACTCAA GATGGTGGAC 
AGCTTTCACA GGGTGAGTCT ACATTATGGG ATTATGTGCC TGAAACGGCT CAACTATGAC 
CGGAAGGACC TGGAGCGGAG GCGGGAAGAA AGTCAGACCC AGATCCGAGA CGTGATGGTG 
TGGTCCAATG AGCGGGTCAT GGGTTGGGTG TCCGGGCTGG GCCTGAAGGA ATTTGCCACG 
AACCTCACGG AGAGCGGGGT ACACGGGGCA CTGCTCGCCC TGGACGAGAC CTTCGACTAC 
TCCGACCTGG CCTTGCTCCT GCAGATCCCC ACGCAGAATG CACAGGCCCG GCAGCTTCTG 
GAGAAGGAAT TCAGCAACCT TATCTCCTTA GGCACAGACA GGCGGCTGGA CGAGGACAGC 
GCCAAGTCTT TCAGCCGCTC CCCATCCTGG CGGAAGATGT TCCGGGAGAA GGACCTCCGA 
GGCGTAACTC CCGACTCAGC TGAGATGTTG CCCCCCAACT TTCGTTCGGC TGCAGCGGGA 
GCCCTGGGCT CTCCGGGGCT CCCTCTCCGC AAGCTGCAGC CAGAAGGCCA GACTTCTGGG 
AGTTCCCGGG CAGACGGCGT TTCGGTCCGG ACCTATTCCT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA3 ( XM_017027407.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882896.1 Homo sapiens PTPRF interacting protein alpha 3 (PPFIA3), transcript variant X1, mRNA.

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CloneID	OHu52559
Clone ID Related Accession (Same CDS sequence)	XM_017027407.1
Accession Version	XM_017027407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	liprin-alpha-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC CACCATCAGC GAGGATGGCC GGCGGGGCTC GGCGCTGGGC 
CCGGACGAGG CGGGCGGGGA GCTGGAGCGC CTCATGGTCA CGATGCTCAC GGAGCGCGAG 
CGCCTGCTGG AGACGCTGCG CGAGGCACAG GACGGGTTGG CTACAGCGCA GCTGCGGCTG 
CGCGAGCTCG GCCACGAGAA GGACTCGCTG CAGCGCCAGC TCAGCATCGC GCTGCCCCAG 
GAGTTTGCAG CTCTGACGAA GGAGCTGAAC TTATGTCGGG AGCAGCTGCT GGAGAGGGAG 
GAAGAGATTG CAGAGCTGAA GGCGGAACGG AACAACACGC GGCTGCTCCT GGAACACCTG 
GAGTGCCTGG TGTCCAGGCA CGAGAGGTCA CTGCGCATGA CCGTGGTGAA GCGCCAGGCC 
CAGTCCCCGG GTGGGGTCTC CTCGGAGGTA GAAGTGCTCA AAGCTCTAAA GTCTCTCTTC 
GAGCACCACA AGGCCCTGGA TGAGAAGGTC CGGGAGCGGC TGCGGATGGC GCTGGAGCGC 
GTGGCAGTGC TCGAGGAGGA GCTGGAACTG AGCAATCAGG AGACTCTGAA CCTTCGAGAA 
CAGCTGTCTA GGCGGCGGTC AGGGCTGGAA GAGCCGGGCA AGGATGGGGA TGGGCAGACT 
CTTGCCAATG GCCTGGGTCC TGGCGGGGAT TCCAACCGGC GCACAGCAGA GCTGGAGGAG 
GCCCTGGAGC GGCAGCGCGC CGAGGTGTGC CAGCTGCGGG AGCGCCTGGC GGTGCTGTGC 
CGTCAGATGA GCCAGCTGGA GGAGGAGTTG GGCACCGCGC ACCGTGAGCT GGGCAAGGCA 
GAGGAAGCCA ACTCCAAGCT GCAGCGCGAC CTCAAGGAGG CGCTGGCGCA GCGGGAAGAT 
ATGGAGGAGC GGATTACAAC ACTGGAGAAG CGCTACCTGA GCGCCCAGCG GGAGGCCACG 
TCTCTGCACG ACGCCAACGA CAAACTGGAG AACGAGTTAG CTAGCAAGGA GTCGTTGTAT 
CGGCAGAGTG AAGAGAAGAG CCGTCAGCTG GCCGAGTGGT TGGACGACGC CAAGCAGAAG 
CTGCAGCAGA CGCTGCAGAA AGCGGAGACC TTGCCCGAGA TAGAGGCGCA GCTGGCGCAG 
CGCGTGGCGG CGCTCAACAA GGCCGAGGAA CGTCATGGGA ATTTTGAGGA GCGGCTTCGG 
CAGCTGGAGG CCCAGCTGGA AGAGAAGAAT CAAGAGCTGC AGCGGGCCCG GCAGCGGGAG 
AAGATGAACG ATGACCACAA TAAGCGGCTG TCCGAGACGG TGGACAAGCT GCTGAGCGAG 
TCCAACGAGC GCTTACAGCT TCACCTCAAG GAGCGCATGG GGGCGCTGGA GGAGAAGAAC 
TCCCTGAGCG AGGAGATAGC CAACATGAAG AAGCTTCAGG ATGAGTTGCT GCTAAACAAG 
GAGCAGCTCT TGGCCGAAAT GGAGCGGATG CAGATGGAGA TCGACCAGCT GCGGGGGAGG 
CCACCATCCT CCTACTCCAG GTCTCTCCCT GGCAGTGCCC TGGAGCTCCG TTACTCTCAG 
GCACCCACTT TACCTTCTGG TGCCCACCTG GATCCCTATG TGGCTGGCAG TGGTCGGGCA 
GGCAAGAGGG GCCGCTGGTC AGGGGTCAAG GAGGAGCCCT CCAAGGATTG GGAGCGGTCT 
GCCCCTGCGG GCTCCATACC ACCCCCATTC CCTGGGGAAC TGGACGGCTC CGATGAGGAG 
GAGGCAGAGG GGATGTTTGG GGCCGAGCTG CTGTCCCCCA GTGGGCAGGC TGACGTGCAG 
ACGCTGGCCA TCATGCTTCA GGAGCAGCTG GAGGCCATCA ACAAGGAGAT CAAGCTGATC 
CAAGAGGAGA AGGAGACAAC AGAACAGAGG GCAGAGGAGC TGGAGAGTCG GGTGTCCAGC 
TCTGGCTTGG ACTCGTTGGG CCGCTACCGC AGCAGCTGCT CCCTGCCCCC CTCCCTCACC 
ACCTCTACCC TTGCCAGCCC CTCCCCTCCC AGCTCTGGCC ACTCAACACC CCGCCTGGCA 
CCCCCTAGCC CTGCCCGTGA GGGCACCGAC AAGGCTAATC ATGTCCCTAA GGAGGAAGCT 
GGAGCTCCAC GAGGGGAGGG GCCGGCCATC CCAGGAGACA CCCCACCACC CACTCCCCGC 
TCTGCCCGTC TTGAGAGAAT GACCCAGGCC TTGGCACTGC AGGCGGGGTC CCTGGAAGAT 
GGGGGACCCC CACGGGGAAG TGAGGGCACC CCAGATTCTC TGCACAAAGC CCCCAAGAAG 
AAGAGCATCA AGTCATCCAT AGGCCGTCTC TTTGGCAAGA AAGAGAAGGG ACGAATGGGA 
CCCCCAGGCC GGGACAGCTC TTCTCTGGCT GGAACACCCT CAGATGAGAC ACTGGCCACT 
GACCCTCTGG GGCTAGCCAA GCTGACAGGC CCAGGAGACA AGGACCGAAG GAACAAGAGG 
AAGCATGAAC TCCTGGAGGA GGCCTGCCGC CAGGGCCTAC CTTTTGCTGC CTGGGACGGG 
CCCACCGTGG TGTCCTGGCT GGAGCTGTGG GTGGGCATGC CTGCCTGGTA TGTGGCCGCC 
TGCCGGGCCA ATGTCAAGAG CGGTGCCATC ATGGCCAACC TGTCAGACAC GGAGATCCAG 
CGCGAGATCG GCATCAGCAA CCCGCTGCAC CGACTCAAGC TACGCCTCGC CATCCAGGAG 
ATGGTCTCGC TCACCTCGCC CTCAGCCCCC GCCTCCTCCC GCACTTCCAC AGGAAACGTG 
TGGATGACAC ACGAGGAGAT GGAGTCCCTT ACGGCCACGA CCAAGCCCAT CCTGGCATAT 
GGCGACATGA ACCACGAGTG GGTGGGGAAC GACTGGCTGC CCAGCCTGGG GCTGCCCCAA 
TACCGCAGCT ACTTCATGGA GTCGCTGGTG GACGCTCGAA TGTTAGATCA CCTTAACAAG 
AAGGAGCTCC GGGGCCAACT CAAGATGGTG GACAGCTTTC ACAGGGTGAG TCTACATTAT 
GGGATTATGT GCCTGAAACG GCTCAACTAT GACCGGAAGG ACCTGGAGCG GAGGCGGGAA 
GAAAGTCAGA CCCAGATCCG AGACGTGATG GTGTGGTCCA ATGAGCGGGT CATGGGTTGG 
GTGTCCGGGC TGGGCCTGAA GGAATTTGCC ACGAACCTCA CGGAGAGCGG GGTACACGGG 
GCACTGCTCG CCCTGGACGA GACCTTCGAC TACTCCGACC TGGCCTTGCT CCTGCAGATC 
CCCACGCAGA ATGCACAGGC CCGGCAGCTT CTGGAGAAGG AATTCAGCAA CCTTATCTCC 
TTAGGCACAG ACAGGCGGCT GGACGAGGAC AGCGCCAAGT CTTTCAGCCG CTCCCCATCC 
TGGCGGAAGA TGTTCCGGGA GAAGGACCTC CGAGGCGTAA CTCCCGACTC AGCTGAGATG 
TTGCCCCCCA ACTTTCGTTC GGCTGCAGCG GGAGCCCTGG GCTCTCCGGG GCTCCCTCTC 
CGCAAGCTGC AGCCAGAAGG CCAGACTTCT GGGAGTTCCC GGGCAGACGG CGTTTCGGTC 
CGGACCTATT CCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882896.1
CDS	350..3907
Translation	MMCEVMPTISEDGRRGSALGPDEAGGELERLMVTMLTERERLLETLREAQDGLATAQLRLRELGHEKDSLQRQLSIALPQEFAALTKELNLCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPGGVSSEVEVLKALKSLFEHHKALDEKVRERLRMALERVAVLEEELELSNQETLNLREQLSRRRSGLEEPGKDGDGQTLANGLGPGGDSNRRTAELEEALERQRAEVCQLRERLAVLCRQMSQLEEELGTAHRELGKAEEANSKLQRDLKEALAQREDMEERITTLEKRYLSAQREATSLHDANDKLENELASKESLYRQSEEKSRQLAEWLDDAKQKLQQTLQKAETLPEIEAQLAQRVAALNKAEERHGNFEERLRQLEAQLEEKNQELQRARQREKMNDDHNKRLSETVDKLLSESNERLQLHLKERMGALEEKNSLSEEIANMKKLQDELLLNKEQLLAEMERMQMEIDQLRGRPPSSYSRSLPGSALELRYSQAPTLPSGAHLDPYVAGSGRAGKRGRWSGVKEEPSKDWERSAPAGSIPPPFPGELDGSDEEEAEGMFGAELLSPSGQADVQTLAIMLQEQLEAINKEIKLIQEEKETTEQRAEELESRVSSSGLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKANHVPKEEAGAPRGEGPAIPGDTPPPTPRSARLERMTQALALQAGSLEDGGPPRGSEGTPDSLHKAPKKKSIKSSIGRLFGKKEKGRMGPPGRDSSSLAGTPSDETLATDPLGLAKLTGPGDKDRRNKRKHELLEEACRQGLPFAAWDGPTVVSWLELWVGMPAWYVAACRANVKSGAIMANLSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPASSRTSTGNVWMTHEEMESLTATTKPILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLNKKELRGQLKMVDSFHRVSLHYGIMCLKRLNYDRKDLERRREESQTQIRDVMVWSNERVMGWVSGLGLKEFATNLTESGVHGALLALDETFDYSDLALLLQIPTQNAQARQLLEKEFSNLISLGTDRRLDEDSAKSFSRSPSWRKMFREKDLRGVTPDSAEMLPPNFRSAAAGALGSPGLPLRKLQPEGQTSGSSRADGVSVRTYSC

Target ORF information:

RefSeq Version	XM_017027407.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PTPRF interacting protein alpha 3 (PPFIA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027407.1

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC CACCATCAGC GAGGATGGCC GGCGGGGCTC GGCGCTGGGC 
CCGGACGAGG CGGGCGGGGA GCTGGAGCGC CTCATGGTCA CGATGCTCAC GGAGCGCGAG 
CGCCTGCTGG AGACGCTGCG CGAGGCACAG GACGGGTTGG CTACAGCGCA GCTGCGGCTG 
CGCGAGCTCG GCCACGAGAA GGACTCGCTG CAGCGCCAGC TCAGCATCGC GCTGCCCCAG 
GAGTTTGCAG CTCTGACGAA GGAGCTGAAC TTATGTCGGG AGCAGCTGCT GGAGAGGGAG 
GAAGAGATTG CAGAGCTGAA GGCGGAACGG AACAACACGC GGCTGCTCCT GGAACACCTG 
GAGTGCCTGG TGTCCAGGCA CGAGAGGTCA CTGCGCATGA CCGTGGTGAA GCGCCAGGCC 
CAGTCCCCGG GTGGGGTCTC CTCGGAGGTA GAAGTGCTCA AAGCTCTAAA GTCTCTCTTC 
GAGCACCACA AGGCCCTGGA TGAGAAGGTC CGGGAGCGGC TGCGGATGGC GCTGGAGCGC 
GTGGCAGTGC TCGAGGAGGA GCTGGAACTG AGCAATCAGG AGACTCTGAA CCTTCGAGAA 
CAGCTGTCTA GGCGGCGGTC AGGGCTGGAA GAGCCGGGCA AGGATGGGGA TGGGCAGACT 
CTTGCCAATG GCCTGGGTCC TGGCGGGGAT TCCAACCGGC GCACAGCAGA GCTGGAGGAG 
GCCCTGGAGC GGCAGCGCGC CGAGGTGTGC CAGCTGCGGG AGCGCCTGGC GGTGCTGTGC 
CGTCAGATGA GCCAGCTGGA GGAGGAGTTG GGCACCGCGC ACCGTGAGCT GGGCAAGGCA 
GAGGAAGCCA ACTCCAAGCT GCAGCGCGAC CTCAAGGAGG CGCTGGCGCA GCGGGAAGAT 
ATGGAGGAGC GGATTACAAC ACTGGAGAAG CGCTACCTGA GCGCCCAGCG GGAGGCCACG 
TCTCTGCACG ACGCCAACGA CAAACTGGAG AACGAGTTAG CTAGCAAGGA GTCGTTGTAT 
CGGCAGAGTG AAGAGAAGAG CCGTCAGCTG GCCGAGTGGT TGGACGACGC CAAGCAGAAG 
CTGCAGCAGA CGCTGCAGAA AGCGGAGACC TTGCCCGAGA TAGAGGCGCA GCTGGCGCAG 
CGCGTGGCGG CGCTCAACAA GGCCGAGGAA CGTCATGGGA ATTTTGAGGA GCGGCTTCGG 
CAGCTGGAGG CCCAGCTGGA AGAGAAGAAT CAAGAGCTGC AGCGGGCCCG GCAGCGGGAG 
AAGATGAACG ATGACCACAA TAAGCGGCTG TCCGAGACGG TGGACAAGCT GCTGAGCGAG 
TCCAACGAGC GCTTACAGCT TCACCTCAAG GAGCGCATGG GGGCGCTGGA GGAGAAGAAC 
TCCCTGAGCG AGGAGATAGC CAACATGAAG AAGCTTCAGG ATGAGTTGCT GCTAAACAAG 
GAGCAGCTCT TGGCCGAAAT GGAGCGGATG CAGATGGAGA TCGACCAGCT GCGGGGGAGG 
CCACCATCCT CCTACTCCAG GTCTCTCCCT GGCAGTGCCC TGGAGCTCCG TTACTCTCAG 
GCACCCACTT TACCTTCTGG TGCCCACCTG GATCCCTATG TGGCTGGCAG TGGTCGGGCA 
GGCAAGAGGG GCCGCTGGTC AGGGGTCAAG GAGGAGCCCT CCAAGGATTG GGAGCGGTCT 
GCCCCTGCGG GCTCCATACC ACCCCCATTC CCTGGGGAAC TGGACGGCTC CGATGAGGAG 
GAGGCAGAGG GGATGTTTGG GGCCGAGCTG CTGTCCCCCA GTGGGCAGGC TGACGTGCAG 
ACGCTGGCCA TCATGCTTCA GGAGCAGCTG GAGGCCATCA ACAAGGAGAT CAAGCTGATC 
CAAGAGGAGA AGGAGACAAC AGAACAGAGG GCAGAGGAGC TGGAGAGTCG GGTGTCCAGC 
TCTGGCTTGG ACTCGTTGGG CCGCTACCGC AGCAGCTGCT CCCTGCCCCC CTCCCTCACC 
ACCTCTACCC TTGCCAGCCC CTCCCCTCCC AGCTCTGGCC ACTCAACACC CCGCCTGGCA 
CCCCCTAGCC CTGCCCGTGA GGGCACCGAC AAGGCTAATC ATGTCCCTAA GGAGGAAGCT 
GGAGCTCCAC GAGGGGAGGG GCCGGCCATC CCAGGAGACA CCCCACCACC CACTCCCCGC 
TCTGCCCGTC TTGAGAGAAT GACCCAGGCC TTGGCACTGC AGGCGGGGTC CCTGGAAGAT 
GGGGGACCCC CACGGGGAAG TGAGGGCACC CCAGATTCTC TGCACAAAGC CCCCAAGAAG 
AAGAGCATCA AGTCATCCAT AGGCCGTCTC TTTGGCAAGA AAGAGAAGGG ACGAATGGGA 
CCCCCAGGCC GGGACAGCTC TTCTCTGGCT GGAACACCCT CAGATGAGAC ACTGGCCACT 
GACCCTCTGG GGCTAGCCAA GCTGACAGGC CCAGGAGACA AGGACCGAAG GAACAAGAGG 
AAGCATGAAC TCCTGGAGGA GGCCTGCCGC CAGGGCCTAC CTTTTGCTGC CTGGGACGGG 
CCCACCGTGG TGTCCTGGCT GGAGCTGTGG GTGGGCATGC CTGCCTGGTA TGTGGCCGCC 
TGCCGGGCCA ATGTCAAGAG CGGTGCCATC ATGGCCAACC TGTCAGACAC GGAGATCCAG 
CGCGAGATCG GCATCAGCAA CCCGCTGCAC CGACTCAAGC TACGCCTCGC CATCCAGGAG 
ATGGTCTCGC TCACCTCGCC CTCAGCCCCC GCCTCCTCCC GCACTTCCAC AGGAAACGTG 
TGGATGACAC ACGAGGAGAT GGAGTCCCTT ACGGCCACGA CCAAGCCCAT CCTGGCATAT 
GGCGACATGA ACCACGAGTG GGTGGGGAAC GACTGGCTGC CCAGCCTGGG GCTGCCCCAA 
TACCGCAGCT ACTTCATGGA GTCGCTGGTG GACGCTCGAA TGTTAGATCA CCTTAACAAG 
AAGGAGCTCC GGGGCCAACT CAAGATGGTG GACAGCTTTC ACAGGGTGAG TCTACATTAT 
GGGATTATGT GCCTGAAACG GCTCAACTAT GACCGGAAGG ACCTGGAGCG GAGGCGGGAA 
GAAAGTCAGA CCCAGATCCG AGACGTGATG GTGTGGTCCA ATGAGCGGGT CATGGGTTGG 
GTGTCCGGGC TGGGCCTGAA GGAATTTGCC ACGAACCTCA CGGAGAGCGG GGTACACGGG 
GCACTGCTCG CCCTGGACGA GACCTTCGAC TACTCCGACC TGGCCTTGCT CCTGCAGATC 
CCCACGCAGA ATGCACAGGC CCGGCAGCTT CTGGAGAAGG AATTCAGCAA CCTTATCTCC 
TTAGGCACAG ACAGGCGGCT GGACGAGGAC AGCGCCAAGT CTTTCAGCCG CTCCCCATCC 
TGGCGGAAGA TGTTCCGGGA GAAGGACCTC CGAGGCGTAA CTCCCGACTC AGCTGAGATG 
TTGCCCCCCA ACTTTCGTTC GGCTGCAGCG GGAGCCCTGG GCTCTCCGGG GCTCCCTCTC 
CGCAAGCTGC AGCCAGAAGG CCAGACTTCT GGGAGTTCCC GGGCAGACGG CGTTTCGGTC 
CGGACCTATT CCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer.
Disease markers20165780538(2016)
Li WH,Zhou ZJ,Huang TH,Guo K,Chen W,Wang Y,Zhang H,Song YC,Chang DM

Liprin ?3: a putative estrogen regulated acrosomal protein.
Histochemistry and cell biology139(4)535-48(2013 Apr)
Joshi CS,Suryawanshi AR,Khan SA,Balasinor NH,Khole VV

Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening.
Proteomics7(11)1775-85(2007 Jun)
Wu C,Ma MH,Brown KR,Geisler M,Li L,Tzeng E,Jia CY,Jurisica I,Li SS

The synaptic vesicle cycle.
Annual review of neuroscience27509-47(2004)
Sudhof TC

Interaction of the ERC family of RIM-binding proteins with the liprin-alpha family of multidomain proteins.
The Journal of biological chemistry278(43)42377-85(2003 Oct)
Ko J,Na M,Kim S,Lee JR,Kim E

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PPFIA3 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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