EAF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EAF1
Entrez Gene ID	85403
Full Name	ELL associated factor 1
General protein information	Preferred Names ELL associated factor 1 Names ELL-associated factor 1 ELL (eleven nineteen lysine-rich leukemia gene)-associated factor 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p25.1
Disorder MIM:	608315

mRNA and Protein(s)

mRNA	Protein	Name
XM_005265518.1	XP_005265575.1	ELL-associated factor 1 isoform X1
NM_033083.7	NP_149074.3	ELL-associated factor 1
NM_033083.6	NP_149074.3	ELL-associated factor 1
XM_005265518.2	XP_005265575.1	ELL-associated factor 1 isoform X1
XM_011534165.1	XP_011532467.1	ELL-associated factor 1 isoform X1
XM_011534166.1	XP_011532468.1	ELL-associated factor 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EAF1	NP_149074.3
Rattus norvegicus (Norway rat)	Eaf1	NP_001100763.1
Pan troglodytes (chimpanzee)	EAF1	XP_001159232.1
	EAF1	XP_001082128.2
Mus musculus (house mouse)	Eaf1	NP_083208.1
Danio rerio (zebrafish)	eaf1	NP_001002115.1
Xenopus tropicalis (tropical clawed frog)	eaf1	NP_001072894.1
Gallus gallus (chicken)	EAF1	XP_001235897.3
Canis lupus familiaris (dog)	EAF1	XP_542776.4
Bos taurus (cattle)	EAF1	NP_001179082.1

Gene Ontology
Bibliography

Related articles in PubMed

Solution Structure of the N-Terminal Domain of Mediator Subunit MED26 and Molecular Characterization of Its Interaction with EAF1 and TAF7.
Lens Z, Cantrelle FX, Peruzzini R, Hanoulle X, Dewitte F, Ferreira E, Baert JL, Mont? D, Aumercier M, Villeret V, Verger A, Landrieu I
Journal of molecular biology429(20)3043-3055(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

The little elongation complex regulates small nuclear RNA transcription.
Smith ER, Lin C, Garrett AS, Thornton J, Mohaghegh N, Hu D, Jackson J, Saraf A, Swanson SK, Seidel C, Florens L, Washburn MP, Eissenberg JC, Shilatifard A
Molecular cell44(6)954-65(2011 Dec)

Human mediator subunit MED26 functions as a docking site for transcription elongation factors.
Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE, Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
Cell146(1)92-104(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EAF1 (239-268) and TAF7 (205-235) peptide interactions were both mapped to the same groove formed by H3 and H4 helices of MED26 N-terminal domain.
Title: Solution Structure of the N-Terminal Domain of Mediator Subunit MED26 and Molecular Characterization of Its Interaction with EAF1 and TAF7.

Findings provide the first convincing line of evidence that EAF and Wnt4 form an auto-regulatory negative feedback loop in vivo.
Title: Negative feedback regulation of Wnt4 signaling by EAF1 and EAF2/U19.

ELL may be an important factor required for U19/Eaf2 function because U19/Eaf2 nuclear localization and transactivation activity are essential for its function as a transcription factor.
Title: ELL binding regulates U19/Eaf2 intracellular localization, stability, and transactivation.

ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL.
Title: ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL.

The following EAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05302	NM_033083.7 Latest version!	Homo sapiens ELL associated factor 1 (EAF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu37532	XM_011534166.1 Latest version!	Homo sapiens ELL associated factor 1 (EAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu37532	XM_005265518.2 Latest version!	Homo sapiens ELL associated factor 1 (EAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu37532	XM_011534165.1 Latest version!	Homo sapiens ELL associated factor 1 (EAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu05302	NM_033083.6	Homo sapiens ELL associated factor 1 (EAF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu37532	XM_005265518.1	Homo sapiens ELL associated factor 1 (EAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EAF1 ( NM_033083.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_149074.3 Homo sapiens ELL associated factor 1 (EAF1), mRNA.

Price & Availability↑

CloneID	OHu05302
Clone ID Related Accession (Same CDS sequence)	NM_033083.7 , NM_033083.6
Accession Version	NM_033083.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	ELL-associated factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL698790.1, AF272973.1, BC041329.2, AC027125.5 and BC035167.2. On Nov 22, 2018 this sequence version replaced NM_033083.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.138232.1, SRR1660805.75989.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000396842.7/ ENSP00000380054.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAATGGGA CCGCAAACCC GCTGCTGGAC CGCGAGGAAC ATTGCCTGAG GCTCGGGGAG 
AGCTTCGAGA AGCGGCCGCG GGCCTCCTTC CACACTATTC GTTATGATTT TAAACCAGCA 
TCTATAGACA CTTCCTGTGA AGGAGAGCTT CAAGTTGGCA AAGGAGATGA AGTCACAATT 
ACACTGCCAC ATATCCCTGG ATCCACACCA CCCATGACTG TGTTCAAGGG GAACAAACGG 
CCTTACCAGA AAGACTGTGT GCTTATTATT AATCATGACA CTGGTGAATA TGTGCTGGAA 
AAACTCAGTA GCAGCATTCA GGTGAAGAAA ACAAGAGCTG AGGGCAGCAG TAAAATCCAG 
GCCCGAATGG AACAGCAGCC CACTCGTCCT CCACAGACGT CACAGCCACC ACCACCTCCA 
CCACCTATGC CATTCAGAGC TCCAACGAAG CCTCCAGTTG GACCCAAAAC TTCTCCCTTG 
AAAGATAACC CCTCACCTGA ACCTCAGTTG GATGACATCA AAAGAGAGCT GAGGGCTGAA 
GTTGACATTA TTGAACAAAT GAGCAGCAGC AGTGGGAGCA GCTCTTCAGA CTCTGAGAGC 
TCTTCGGGAA GTGATGACGA TAGCTCCAGC AGTGGAGGCG AGGACAATGG CCCAGCCTCT 
CCTCCGCAGC CTTCACACCA GCAGCCCTAC AACAGTAGGC CTGCCGTTGC CAATGGAACC 
AGCCGGCCAC AAGGAAGCAA CCAGCTCATG AACACCCTCA GAAATGACTT GCAGTTGAGT 
GAGTCTGGCA GTGACAGTGA TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149074.3
CDS	183..989
Translation	MNGTANPLLDREEHCLRLGESFEKRPRASFHTIRYDFKPASIDTSCEGELQVGKGDEVTITLPHIPGSTPPMTVFKGNKRPYQKDCVLIINHDTGEYVLEKLSSSIQVKKTRAEGSSKIQARMEQQPTRPPQTSQPPPPPPPMPFRAPTKPPVGPKTSPLKDNPSPEPQLDDIKRELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSSSGGEDNGPASPPQPSHQQPYNSRPAVANGTSRPQGSNQLMNTLRNDLQLSESGSDSDD

Target ORF information:

RefSeq Version	NM_033083.7
Organism	Homo sapiens(human)
Definition	Homo sapiens ELL associated factor 1 (EAF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033083.7

ORF Insert Sequence:

ATGAATGGGA CCGCAAACCC GCTGCTGGAC CGCGAGGAAC ATTGCCTGAG GCTCGGGGAG 
AGCTTCGAGA AGCGGCCGCG GGCCTCCTTC CACACTATTC GTTATGATTT TAAACCAGCA 
TCTATAGACA CTTCCTGTGA AGGAGAGCTT CAAGTTGGCA AAGGAGATGA AGTCACAATT 
ACACTGCCAC ATATCCCTGG ATCCACACCA CCCATGACTG TGTTCAAGGG GAACAAACGG 
CCTTACCAGA AAGACTGTGT GCTTATTATT AATCATGACA CTGGTGAATA TGTGCTGGAA 
AAACTCAGTA GCAGCATTCA GGTGAAGAAA ACAAGAGCTG AGGGCAGCAG TAAAATCCAG 
GCCCGAATGG AACAGCAGCC CACTCGTCCT CCACAGACGT CACAGCCACC ACCACCTCCA 
CCACCTATGC CATTCAGAGC TCCAACGAAG CCTCCAGTTG GACCCAAAAC TTCTCCCTTG 
AAAGATAACC CCTCACCTGA ACCTCAGTTG GATGACATCA AAAGAGAGCT GAGGGCTGAA 
GTTGACATTA TTGAACAAAT GAGCAGCAGC AGTGGGAGCA GCTCTTCAGA CTCTGAGAGC 
TCTTCGGGAA GTGATGACGA TAGCTCCAGC AGTGGAGGCG AGGACAATGG CCCAGCCTCT 
CCTCCGCAGC CTTCACACCA GCAGCCCTAC AACAGTAGGC CTGCCGTTGC CAATGGAACC 
AGCCGGCCAC AAGGAAGCAA CCAGCTCATG AACACCCTCA GAAATGACTT GCAGTTGAGT 
GAGTCTGGCA GTGACAGTGA TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EAF1 ( NM_033083.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_149074.3 Homo sapiens ELL associated factor 1 (EAF1), mRNA.

Price & Availability↑

CloneID	OHu05302
Clone ID Related Accession (Same CDS sequence)	NM_033083.7 , NM_033083.6
Accession Version	NM_033083.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Homo sapiens(human)
Product	ELL-associated factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL698790.1, AF272973.1, BC041329.2, AC027125.5 and BC035167.2. On Jun 16, 2006 this sequence version replaced NM_033083.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.235711.1, SRR1660803.138232.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATGGGA CCGCAAACCC GCTGCTGGAC CGCGAGGAAC ATTGCCTGAG GCTCGGGGAG 
AGCTTCGAGA AGCGGCCGCG GGCCTCCTTC CACACTATTC GTTATGATTT TAAACCAGCA 
TCTATAGACA CTTCCTGTGA AGGAGAGCTT CAAGTTGGCA AAGGAGATGA AGTCACAATT 
ACACTGCCAC ATATCCCTGG ATCCACACCA CCCATGACTG TGTTCAAGGG GAACAAACGG 
CCTTACCAGA AAGACTGTGT GCTTATTATT AATCATGACA CTGGTGAATA TGTGCTGGAA 
AAACTCAGTA GCAGCATTCA GGTGAAGAAA ACAAGAGCTG AGGGCAGCAG TAAAATCCAG 
GCCCGAATGG AACAGCAGCC CACTCGTCCT CCACAGACGT CACAGCCACC ACCACCTCCA 
CCACCTATGC CATTCAGAGC TCCAACGAAG CCTCCAGTTG GACCCAAAAC TTCTCCCTTG 
AAAGATAACC CCTCACCTGA ACCTCAGTTG GATGACATCA AAAGAGAGCT GAGGGCTGAA 
GTTGACATTA TTGAACAAAT GAGCAGCAGC AGTGGGAGCA GCTCTTCAGA CTCTGAGAGC 
TCTTCGGGAA GTGATGACGA TAGCTCCAGC AGTGGAGGCG AGGACAATGG CCCAGCCTCT 
CCTCCGCAGC CTTCACACCA GCAGCCCTAC AACAGTAGGC CTGCCGTTGC CAATGGAACC 
AGCCGGCCAC AAGGAAGCAA CCAGCTCATG AACACCCTCA GAAATGACTT GCAGTTGAGT 
GAGTCTGGCA GTGACAGTGA TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149074.3
CDS	224..1030
Translation	MNGTANPLLDREEHCLRLGESFEKRPRASFHTIRYDFKPASIDTSCEGELQVGKGDEVTITLPHIPGSTPPMTVFKGNKRPYQKDCVLIINHDTGEYVLEKLSSSIQVKKTRAEGSSKIQARMEQQPTRPPQTSQPPPPPPPMPFRAPTKPPVGPKTSPLKDNPSPEPQLDDIKRELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSSSGGEDNGPASPPQPSHQQPYNSRPAVANGTSRPQGSNQLMNTLRNDLQLSESGSDSDD

Target ORF information:

RefSeq Version	NM_033083.6
Organism	Homo sapiens(human)
Definition	Homo sapiens ELL associated factor 1 (EAF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033083.6

ORF Insert Sequence:

ATGAATGGGA CCGCAAACCC GCTGCTGGAC CGCGAGGAAC ATTGCCTGAG GCTCGGGGAG 
AGCTTCGAGA AGCGGCCGCG GGCCTCCTTC CACACTATTC GTTATGATTT TAAACCAGCA 
TCTATAGACA CTTCCTGTGA AGGAGAGCTT CAAGTTGGCA AAGGAGATGA AGTCACAATT 
ACACTGCCAC ATATCCCTGG ATCCACACCA CCCATGACTG TGTTCAAGGG GAACAAACGG 
CCTTACCAGA AAGACTGTGT GCTTATTATT AATCATGACA CTGGTGAATA TGTGCTGGAA 
AAACTCAGTA GCAGCATTCA GGTGAAGAAA ACAAGAGCTG AGGGCAGCAG TAAAATCCAG 
GCCCGAATGG AACAGCAGCC CACTCGTCCT CCACAGACGT CACAGCCACC ACCACCTCCA 
CCACCTATGC CATTCAGAGC TCCAACGAAG CCTCCAGTTG GACCCAAAAC TTCTCCCTTG 
AAAGATAACC CCTCACCTGA ACCTCAGTTG GATGACATCA AAAGAGAGCT GAGGGCTGAA 
GTTGACATTA TTGAACAAAT GAGCAGCAGC AGTGGGAGCA GCTCTTCAGA CTCTGAGAGC 
TCTTCGGGAA GTGATGACGA TAGCTCCAGC AGTGGAGGCG AGGACAATGG CCCAGCCTCT 
CCTCCGCAGC CTTCACACCA GCAGCCCTAC AACAGTAGGC CTGCCGTTGC CAATGGAACC 
AGCCGGCCAC AAGGAAGCAA CCAGCTCATG AACACCCTCA GAAATGACTT GCAGTTGAGT 
GAGTCTGGCA GTGACAGTGA TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EAF1 ( XM_005265518.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005265575.1 Homo sapiens ELL associated factor 1 (EAF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu37532
Clone ID Related Accession (Same CDS sequence)	XM_005265518.1 , XM_005265518.2 , XM_011534165.1 , XM_011534166.1
Accession Version	XM_005265518.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ELL-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAC AATTACACTG CCACATATCC CTAGCTGAGG GCAGCAGTAA AATCCAGGCC 
CGAATGGAAC AGCAGCCCAC TCGTCCTCCA CAGACGTCAC AGCCACCACC ACCTCCACCA 
CCTATGCCAT TCAGAGCTCC AACGAAGCCT CCAGTTGGAC CCAAAACTTC TCCCTTGAAA 
GATAACCCCT CACCTGAACC TCAGTTGGAT GACATCAAAA GAGAGCTGAG GGCTGAAGTT 
GACATTATTG AACAAATGAG CAGCAGCAGT GGGAGCAGCT CTTCAGACTC TGAGAGCTCT 
TCGGGAAGTG ATGACGATAG CTCCAGCAGT GGAGGCGAGG ACAATGGCCC AGCCTCTCCT 
CCGCAGCCTT CACACCAGCA GCCCTACAAC AGTAGGCCTG CCGTTGCCAA TGGAACCAGC 
CGGCCACAAG GAAGCAACCA GCTCATGAAC ACCCTCAGAA ATGACTTGCA GTTGAGTGAG 
TCTGGCAGTG ACAGTGATGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005265575.1
CDS	390..893
Translation	MKSQLHCHISLAEGSSKIQARMEQQPTRPPQTSQPPPPPPPMPFRAPTKPPVGPKTSPLKDNPSPEPQLDDIKRELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSSSGGEDNGPASPPQPSHQQPYNSRPAVANGTSRPQGSNQLMNTLRNDLQLSESGSDSDD

Target ORF information:

RefSeq Version	XM_005265518.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ELL associated factor 1 (EAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005265518.1

ORF Insert Sequence:

ATGAAGTCAC AATTACACTG CCACATATCC CTAGCTGAGG GCAGCAGTAA AATCCAGGCC 
CGAATGGAAC AGCAGCCCAC TCGTCCTCCA CAGACGTCAC AGCCACCACC ACCTCCACCA 
CCTATGCCAT TCAGAGCTCC AACGAAGCCT CCAGTTGGAC CCAAAACTTC TCCCTTGAAA 
GATAACCCCT CACCTGAACC TCAGTTGGAT GACATCAAAA GAGAGCTGAG GGCTGAAGTT 
GACATTATTG AACAAATGAG CAGCAGCAGT GGGAGCAGCT CTTCAGACTC TGAGAGCTCT 
TCGGGAAGTG ATGACGATAG CTCCAGCAGT GGAGGCGAGG ACAATGGCCC AGCCTCTCCT 
CCGCAGCCTT CACACCAGCA GCCCTACAAC AGTAGGCCTG CCGTTGCCAA TGGAACCAGC 
CGGCCACAAG GAAGCAACCA GCTCATGAAC ACCCTCAGAA ATGACTTGCA GTTGAGTGAG 
TCTGGCAGTG ACAGTGATGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EAF1 ( XM_005265518.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005265575.1 Homo sapiens ELL associated factor 1 (EAF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu37532
Clone ID Related Accession (Same CDS sequence)	XM_005265518.1 , XM_005265518.2 , XM_011534165.1 , XM_011534166.1
Accession Version	XM_005265518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ELL-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005265518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAC AATTACACTG CCACATATCC CTAGCTGAGG GCAGCAGTAA AATCCAGGCC 
CGAATGGAAC AGCAGCCCAC TCGTCCTCCA CAGACGTCAC AGCCACCACC ACCTCCACCA 
CCTATGCCAT TCAGAGCTCC AACGAAGCCT CCAGTTGGAC CCAAAACTTC TCCCTTGAAA 
GATAACCCCT CACCTGAACC TCAGTTGGAT GACATCAAAA GAGAGCTGAG GGCTGAAGTT 
GACATTATTG AACAAATGAG CAGCAGCAGT GGGAGCAGCT CTTCAGACTC TGAGAGCTCT 
TCGGGAAGTG ATGACGATAG CTCCAGCAGT GGAGGCGAGG ACAATGGCCC AGCCTCTCCT 
CCGCAGCCTT CACACCAGCA GCCCTACAAC AGTAGGCCTG CCGTTGCCAA TGGAACCAGC 
CGGCCACAAG GAAGCAACCA GCTCATGAAC ACCCTCAGAA ATGACTTGCA GTTGAGTGAG 
TCTGGCAGTG ACAGTGATGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005265575.1
CDS	332..835
Translation	MKSQLHCHISLAEGSSKIQARMEQQPTRPPQTSQPPPPPPPMPFRAPTKPPVGPKTSPLKDNPSPEPQLDDIKRELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSSSGGEDNGPASPPQPSHQQPYNSRPAVANGTSRPQGSNQLMNTLRNDLQLSESGSDSDD

Target ORF information:

RefSeq Version	XM_005265518.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ELL associated factor 1 (EAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005265518.2

ORF Insert Sequence:

ATGAAGTCAC AATTACACTG CCACATATCC CTAGCTGAGG GCAGCAGTAA AATCCAGGCC 
CGAATGGAAC AGCAGCCCAC TCGTCCTCCA CAGACGTCAC AGCCACCACC ACCTCCACCA 
CCTATGCCAT TCAGAGCTCC AACGAAGCCT CCAGTTGGAC CCAAAACTTC TCCCTTGAAA 
GATAACCCCT CACCTGAACC TCAGTTGGAT GACATCAAAA GAGAGCTGAG GGCTGAAGTT 
GACATTATTG AACAAATGAG CAGCAGCAGT GGGAGCAGCT CTTCAGACTC TGAGAGCTCT 
TCGGGAAGTG ATGACGATAG CTCCAGCAGT GGAGGCGAGG ACAATGGCCC AGCCTCTCCT 
CCGCAGCCTT CACACCAGCA GCCCTACAAC AGTAGGCCTG CCGTTGCCAA TGGAACCAGC 
CGGCCACAAG GAAGCAACCA GCTCATGAAC ACCCTCAGAA ATGACTTGCA GTTGAGTGAG 
TCTGGCAGTG ACAGTGATGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EAF1 ( XM_011534165.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532467.1 Homo sapiens ELL associated factor 1 (EAF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu37532
Clone ID Related Accession (Same CDS sequence)	XM_005265518.1 , XM_005265518.2 , XM_011534165.1 , XM_011534166.1
Accession Version	XM_011534165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ELL-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAC AATTACACTG CCACATATCC CTAGCTGAGG GCAGCAGTAA AATCCAGGCC 
CGAATGGAAC AGCAGCCCAC TCGTCCTCCA CAGACGTCAC AGCCACCACC ACCTCCACCA 
CCTATGCCAT TCAGAGCTCC AACGAAGCCT CCAGTTGGAC CCAAAACTTC TCCCTTGAAA 
GATAACCCCT CACCTGAACC TCAGTTGGAT GACATCAAAA GAGAGCTGAG GGCTGAAGTT 
GACATTATTG AACAAATGAG CAGCAGCAGT GGGAGCAGCT CTTCAGACTC TGAGAGCTCT 
TCGGGAAGTG ATGACGATAG CTCCAGCAGT GGAGGCGAGG ACAATGGCCC AGCCTCTCCT 
CCGCAGCCTT CACACCAGCA GCCCTACAAC AGTAGGCCTG CCGTTGCCAA TGGAACCAGC 
CGGCCACAAG GAAGCAACCA GCTCATGAAC ACCCTCAGAA ATGACTTGCA GTTGAGTGAG 
TCTGGCAGTG ACAGTGATGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532467.1
CDS	539..1042
Translation	MKSQLHCHISLAEGSSKIQARMEQQPTRPPQTSQPPPPPPPMPFRAPTKPPVGPKTSPLKDNPSPEPQLDDIKRELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSSSGGEDNGPASPPQPSHQQPYNSRPAVANGTSRPQGSNQLMNTLRNDLQLSESGSDSDD

Target ORF information:

RefSeq Version	XM_011534165.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ELL associated factor 1 (EAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534165.1

ORF Insert Sequence:

ATGAAGTCAC AATTACACTG CCACATATCC CTAGCTGAGG GCAGCAGTAA AATCCAGGCC 
CGAATGGAAC AGCAGCCCAC TCGTCCTCCA CAGACGTCAC AGCCACCACC ACCTCCACCA 
CCTATGCCAT TCAGAGCTCC AACGAAGCCT CCAGTTGGAC CCAAAACTTC TCCCTTGAAA 
GATAACCCCT CACCTGAACC TCAGTTGGAT GACATCAAAA GAGAGCTGAG GGCTGAAGTT 
GACATTATTG AACAAATGAG CAGCAGCAGT GGGAGCAGCT CTTCAGACTC TGAGAGCTCT 
TCGGGAAGTG ATGACGATAG CTCCAGCAGT GGAGGCGAGG ACAATGGCCC AGCCTCTCCT 
CCGCAGCCTT CACACCAGCA GCCCTACAAC AGTAGGCCTG CCGTTGCCAA TGGAACCAGC 
CGGCCACAAG GAAGCAACCA GCTCATGAAC ACCCTCAGAA ATGACTTGCA GTTGAGTGAG 
TCTGGCAGTG ACAGTGATGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EAF1 ( XM_011534166.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532468.1 Homo sapiens ELL associated factor 1 (EAF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu37532
Clone ID Related Accession (Same CDS sequence)	XM_005265518.1 , XM_005265518.2 , XM_011534165.1 , XM_011534166.1
Accession Version	XM_011534166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ELL-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAC AATTACACTG CCACATATCC CTAGCTGAGG GCAGCAGTAA AATCCAGGCC 
CGAATGGAAC AGCAGCCCAC TCGTCCTCCA CAGACGTCAC AGCCACCACC ACCTCCACCA 
CCTATGCCAT TCAGAGCTCC AACGAAGCCT CCAGTTGGAC CCAAAACTTC TCCCTTGAAA 
GATAACCCCT CACCTGAACC TCAGTTGGAT GACATCAAAA GAGAGCTGAG GGCTGAAGTT 
GACATTATTG AACAAATGAG CAGCAGCAGT GGGAGCAGCT CTTCAGACTC TGAGAGCTCT 
TCGGGAAGTG ATGACGATAG CTCCAGCAGT GGAGGCGAGG ACAATGGCCC AGCCTCTCCT 
CCGCAGCCTT CACACCAGCA GCCCTACAAC AGTAGGCCTG CCGTTGCCAA TGGAACCAGC 
CGGCCACAAG GAAGCAACCA GCTCATGAAC ACCCTCAGAA ATGACTTGCA GTTGAGTGAG 
TCTGGCAGTG ACAGTGATGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532468.1
CDS	534..1037
Translation	MKSQLHCHISLAEGSSKIQARMEQQPTRPPQTSQPPPPPPPMPFRAPTKPPVGPKTSPLKDNPSPEPQLDDIKRELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSSSGGEDNGPASPPQPSHQQPYNSRPAVANGTSRPQGSNQLMNTLRNDLQLSESGSDSDD

Target ORF information:

RefSeq Version	XM_011534166.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ELL associated factor 1 (EAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534166.1

ORF Insert Sequence:

ATGAAGTCAC AATTACACTG CCACATATCC CTAGCTGAGG GCAGCAGTAA AATCCAGGCC 
CGAATGGAAC AGCAGCCCAC TCGTCCTCCA CAGACGTCAC AGCCACCACC ACCTCCACCA 
CCTATGCCAT TCAGAGCTCC AACGAAGCCT CCAGTTGGAC CCAAAACTTC TCCCTTGAAA 
GATAACCCCT CACCTGAACC TCAGTTGGAT GACATCAAAA GAGAGCTGAG GGCTGAAGTT 
GACATTATTG AACAAATGAG CAGCAGCAGT GGGAGCAGCT CTTCAGACTC TGAGAGCTCT 
TCGGGAAGTG ATGACGATAG CTCCAGCAGT GGAGGCGAGG ACAATGGCCC AGCCTCTCCT 
CCGCAGCCTT CACACCAGCA GCCCTACAAC AGTAGGCCTG CCGTTGCCAA TGGAACCAGC 
CGGCCACAAG GAAGCAACCA GCTCATGAAC ACCCTCAGAA ATGACTTGCA GTTGAGTGAG 
TCTGGCAGTG ACAGTGATGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Solution Structure of the N-Terminal Domain of Mediator Subunit MED26 and Molecular Characterization of Its Interaction with EAF1 and TAF7.
Journal of molecular biology429(20)3043-3055(2017 Oct)
Lens Z,Cantrelle FX,Peruzzini R,Hanoulle X,Dewitte F,Ferreira E,Baert JL,Mont? D,Aumercier M,Villeret V,Verger A,Landrieu I

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

The little elongation complex regulates small nuclear RNA transcription.
Molecular cell44(6)954-65(2011 Dec)
Smith ER,Lin C,Garrett AS,Thornton J,Mohaghegh N,Hu D,Jackson J,Saraf A,Swanson SK,Seidel C,Florens L,Washburn MP,Eissenberg JC,Shilatifard A

Human mediator subunit MED26 functions as a docking site for transcription elongation factors.
Cell146(1)92-104(2011 Jul)
Takahashi H,Parmely TJ,Sato S,Tomomori-Sato C,Banks CA,Kong SE,Szutorisz H,Swanson SK,Martin-Brown S,Washburn MP,Florens L,Seidel CW,Lin C,Smith ER,Shilatifard A,Conaway RC,Conaway JW

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EAF1 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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