TUBGCP6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TUBGCP6
Entrez Gene ID	85378
Full Name	tubulin gamma complex associated protein 6
Synonyms	GCP-6,GCP6,MCCRP,MCCRP1,MCPHCR
General protein information	Preferred Names tubulin gamma complex associated protein 6 Names gamma-tubulin complex component 6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.33
Summary	The protein encoded by this gene is part of a large multisubunit complex required for microtubule nucleation at the centrosome. [provided by RefSeq, Jul 2008].
Disorder MIM:	610053
Disorder Html:	Microcephaly and chorioretinopathy, autosomal recessive, 1, 251270 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_020461.4	NP_065194.3	gamma-tubulin complex component 6
NM_020461.3	NP_065194.2	gamma-tubulin complex component 6

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TUBGCP6	XP_002688047.1
Homo sapiens (human)	TUBGCP6	NP_065194.2
Mus musculus (house mouse)	Tubgcp6	NP_001156791.1
Gallus gallus (chicken)	TUBGCP6	XP_001232991.3
Canis lupus familiaris (dog)	TUBGCP6	XP_005625769.1
Rattus norvegicus (Norway rat)	Tubgcp6	NP_001102218.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Navarini AA, Simpson MA, Weale M, Knight J, Carlavan I, Reiniche P, Burden DA, Layton A, Bataille V, Allen M, Pleass R, Pink A, Creamer D, English J, Munn S, Walton S, , Willis C, D?ret S, Voegel JJ, Spector T, Smith CH, Trembath RC, Barker JN
Nature communications54020(2014 Jun)

Gene network analysis in a pediatric cohort identifies novel lung function genes.
Ong BA, Li J, McDonough JM, Wei Z, Kim C, Chiavacci R, Mentch F, Caboot JB, Spergel J, Allen JL, Sleiman PM, Hakonarson H
PloS one8(9)e72899(2013)

GCP6 is a substrate of Plk4 and required for centriole duplication.
Bahtz R, Seidler J, Arnold M, Haselmann-Weiss U, Antony C, Lehmann WD, Hoffmann I
Journal of cell science125(Pt 2)486-96(2012 Jan)

Genetic mapping and exome sequencing identify variants associated with five novel diseases.
Puffenberger EG, Jinks RN, Sougnez C, Cibulskis K, Willert RA, Achilly NP, Cassidy RP, Fiorentini CJ, Heiken KF, Lawrence JJ, Mahoney MH, Miller CJ, Nair DT, Politi KA, Worcester KN, Setton RA, Dipiazza R, Sherman EA, Eastman JT, Francklyn C, Robey-Bond S, Rider NL, Gabriel S, Morton DH, Strauss KA
PloS one7(1)e28936(2012)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data suggest that GCP6 is a target of Plk4 in centriole biogenesis.
Title: GCP6 is a substrate of Plk4 and required for centriole duplication.

Study identified sequence variants in the known disease-causing genes SLC6A3 and FLVCR1, and present evidence to strongly support the pathogenicity of variants identified in TUBGCP6, BRAT1, SNIP1, CRADD, and HARS.
Title: Genetic mapping and exome sequencing identify variants associated with five novel diseases.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Host determinants of HIV-1 control in African Americans.

GCP6 participates in the attachment of microtubule-organizing centers to intermediate filaments in epithelial cells and is among the factors that determine the peculiar architecture of microtubules in polarized epithelia.
Title: GCP6 binds to intermediate filaments: a novel function of keratins in the organization of microtubules in epithelial cells.

The following TUBGCP6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUBGCP6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu107319	NM_020461.4 Latest version!	Homo sapiens tubulin gamma complex associated protein 6 (TUBGCP6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu17827	NM_020461.3 Latest version!	Homo sapiens tubulin gamma complex associated protein 6 (TUBGCP6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TUBGCP6 ( NM_020461.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065194.3 Homo sapiens tubulin gamma complex associated protein 6 (TUBGCP6), mRNA.

Price & Availability↑

CloneID	OHu107319
Clone ID Related Accession (Same CDS sequence)	NM_020461.4
Accession Version	NM_020461.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	gamma-tubulin complex component 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL022328.21. On Nov 23, 2018 this sequence version replaced NM_020461.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF272887.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000248846.10/ ENSP00000248846.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAGCA TCACGCAGCT GTTCGACGAC CTGTGTGAGG CCCTCCTGCC GGCTGCCAAG 
ACTCACCTGG GCCAGCGCAG TGTGAACCGG AAGAGGGCAA AGCGGAGCCT CAAGAAGGTG 
GCCTACAATG CTCTTTTCAC AAATCTTTTT CAAGATGAGA CTCAACAGCT GCAGCCTGAC 
ATGTCAAAAC TACCAGCGAG AAACAAGATC CTCATGTTGT CCTTTGACTT GAGAGTGGGT 
GGCCTGGGCC CCAAGGCCGA CCGTTTGGAG GAGCTTGTGG AGGAGCTGGA AGCAGCCCCT 
TGCTGTCCGC TTTTGGAGGT GGGGTCTGTT TTGGACCTCC TGGTTCAGCT GGCAGGGAGT 
GGTCCCCCTC AAGTTCTGCC GAGAAAACGA GACTACTTCC TTAACAACAA GCATGTGGGG 
AGAAACGTTC CGTACAGCGG CTATGATTGC GACGACCTGA GTGTGTTTGA GATGGACGTT 
CAGTCTCTGA TCTCCAGAGA AGAGTGTTTG TGTCACAGCA TGATCCAGGA AACACTTCAG 
GTTATGGAGG CTGCTCCAGG CACTGGCCTG CCCACCGTCG GGCTCTTCTC ATTTGGTGAC 
CCTTGTGGTG ACAGGTTCGA GAGAGACACC CGGGTCTCGC TCTTCGGGGC CCTTGTGCAC 
AGCCGCACTT ATGACATGGA CGTCCGACTG GGCCTGCCCC CCGTGCCAGA CAATGCGGAC 
CTCTCTGGGC TGGCTATTAA GGTCCCACCG AGTGTGGATC AGTGGGAAGA CGAAGGATTC 
CAGTCAGCGT CCAACTTGAC TCCTGATTCC CAGTCTGAGC CCAGCGTGAC CCCAGACGTG 
GACCTGTGGG AAGCCGCACT TACCTATGAG GCCAGCAAGC GGAGGTGCTG GGAGCGAGTT 
GGCTGCCCCC CTGGCCACAG AGAGGAGCCT TACCTGACGG AGGCGGGAAG GGACGCTTTC 
GACAAGTTCT GCAGGCTCCA CCAAGGGGAG CTCCAGCTGC TTGCTGGGGG CGTCCTACAG 
GCCCCGCAGC CCGTGCTGGT GAAGGAGTGC GAGCTGGTGA AAGACGTGCT GAACGTCTTG 
ATTGGGGTCG TGTCTGCCAC GTTTTCGCTC TGCCAGCCGG CCCAGGCCTT TGTGGTGAAG 
CGGGGCGTCC ACGTGTCAGG AGCGTCTCCC GAGAGCATCA GCAGCCTGCT CTCGGAAGTG 
GCCGAGTATG GGACCTGCTA CACGCGCCTG AGTCATTTCT CTCTGCAGCC CGTCCTGGAC 
TCTTTGTACA GCAAGGGCCT CGTGTTCCAG GCCTTCACCA GTGGCCTGAG GAGGTACCTG 
CAGTATTACC GGGCCTGCGT CCTTTCCACT CCGCCCACCC TGAGCCTCCT CACCATTGGT 
TTTCTCTTCA AGAAACTTGG CCGGCAGCTC AGGTACCTGG CCGAGCTCTG TGGCGTTGGC 
GCTGTGCTCC CGGGCACCTG TGGAGGAGGC CCCAGGGCCG CGTTTCCCAC CGGCGTGAAG 
CTGCTGTCCT ACCTCTACCA GGAGGCTCTG CACAACTGCA GCAACGAGCA CTACCCTGTA 
CTGCTGTCCC TGCTGAAGAC CAGCTGCGAG CCCTACACCC GGTTCATCCA CGACTGGGTG 
TACAGCGGGG TGTTCAGAGA CGCTTATGGC GAGTTCATGA TTCAGGTGAA CCACGAGTAC 
CTCAGCTTCC GAGATAAGTT GTACTGGACA CATGGCTACG TGCTCATCTC CAAAGAGGTG 
GAGGACTGTG TTCCCGTGTT TCTGAAGCAC ATTGCCCACG ACATATACGT CTGCGGAAAG 
ACCATTAACC TGCTGAAGCT CTGCTGCCCC CGGCATTACC TCTGTTGGTC CGACGTCCCT 
GTCCCCCGGA TCTCGGTGAT TTTCTCCCTT GAGGAGTTGA AGGAGATTGA GAAGGACTGT 
GCCGTCTACG TTGGGCGCAT GGAGAGGGTG GCCCGCCACA GCTCTGTCAG CAAGGAGGAG 
AAGGAATTAC GTATGGAAAT TGCAAAACAA GAATTAATCG CTCATGCCCG GGAAGCAGCA 
TCCAGGGTCC TGAGTGCACT GAGTGACCGG CAGATGTCAG AACGGATGGC CTTGGATGCC 
CGGAAGCGTG AGCAGTTTCA GAGGCTGAAA GAACAATTTG TGAAGGACCA GGAGCGACGC 
CAGGCGGCCA GGCAGGAGGA GCTGGATGAT GACTTCAGCT ACGCCCGTGA ACTCCGAGAC 
AGGGAGAGAA GGCTGAAGTC CCTGGAGGAG GAGCTGGAGA GGAAGGCGAG GCAGGCACTG 
GTCGACCACT ACAGCAAGCT CTCTGCAGAG GCAGCTCGTC GGGAGCAGAA GGCACTGTGG 
AGAATCCAGA GGCACCGACT GGAGAGTGCA CGGCTTCGTT TTCTCTTAGA AGATGAGAAA 
CACATTCAGG AGATGCTGAA AGCAGTGTCT GAGGCTCACC AGCCCCAGGA GCCGCCAGAC 
GTCCTCTTGA GCGTGCACCC CCAGGTCACG TCTCCGGGCC CTGAGCACCC AGAGGGAGGC 
CAAGGCTGTG ATTCTGGGTC TGCAGAGCAA CACTCGCCTG CCTGGGATGG CTGGAACAGG 
CCAGGCCTGC TGACCCCACA GCCCCTTAAG CCTCTAGCAG TGGGGGCTGG TGGCAGGGGG 
CTGCAGCAGG CGGAGGGGGC CAGACCCTTC TCTGACAGCC TCAGCATTGG AGACTTCCTA 
CCTGTGGGCC CAGGGGCTGA GCCGTCCGTG CAGACTGGCA TGGTCCCTCT CCTGGAGGTG 
GCGCTGCAGA CCATTAACTT GGACCTGCCC CCCTCAGCTC CTGGGGAGGC ACCCGCAGCA 
GCCAGCACTC AGCCCTCCAG GCCACAGGAG TACGACTTTA GTACTGTCCT GAGGCCAGCT 
GTGGCCACCT CACCTGCACC AGGGCCCCTG CAGGCTGCAG AGTGCAGCTT GGGCAGCTCA 
GGGCTACAGC TGTGGGAGGA CAGTTGTGGG AAGATGGATG CCTGTGGCTC CGCCTCGCGG 
GAGACTCTGC TCCCCTCACA CCCACCCAGG CGTGCTGCTC TGGAGGAGGG GAGCAGCCAG 
CCCACAGAGC GGCTCTTTGG GCAGGTGTCA GGGGGTGGTC TTCCCACAGG GGACTACGCT 
TCTGAAATAG CTCCCACCCG GCCACGGTGG AACACCCACG GGCACGTGTC TGACGCCAGC 
ATCAGGGTCG GGGAGAATGT GTCAGATGTG GCTCCCACCC AGCCACGGTG GAACACCCAC 
GGACACGTAT CCAATGCCAG CATCAGCTTA GGGGAGTCTG TGTCAGATGT GGCTCCCACT 
CGGCCACGGT GGAACATCCA TGGACATGTG TCCAACGCCA GCATCAGGGT CGGGGAGAAT 
GTGTCAGATG TGGCTCCCAC CAGGCCACGG TGGAATACCC ACGGGCACGT GTCCAACGCC 
AGCATCAGGG TTGGGGAGAA CGTGTCGGAC GTGGCTCCCA CCCGGCCACG GTGGAACACC 
CATGGACACG TGTCCGATGC CAGCATCAGC TTGGGGGAGT CTGTGTCAGA CATGGCTCCC 
GCCCGGCCAC GGTGGAACAC CCATGGACAC GTGTCTGATG CCAGCATCAG CTTGGGGGAG 
TCTGTGTCAG ACATGGCTCC CACCCGGCCA CGGTGGAACA CCCACGGACA TGTGTCTGAC 
ACCAGCATCA GGGTTGGGGA GAACGTATCG GACGTGGCTC CCATCCGGTC ACGGTGCAAC 
ACCCATGGAC ACGTGTCCGA CGCCAGCATC AGCTTGGGGG AGCCTGTGTC GGATGTGGTT 
TCCACCCGGC CACGGTGGAA CACCCATGTA CCCATCCCTC CGCCCCACAT GGTGCTGGGG 
GCTCTCTCAC CAGAAGCTGA GCCCAACACA CCCAGGCCCC AACAGAGCCC CCCTGGCCAC 
ACGTCCCAGT CAGCGCTCAG CCTGGGAGCA CAGAGCACTG TGCTGGACTG TGGGCCACGG 
CTGCCTGTAG AAGTGGGGCC ATCTCTGTCC TCCCCCAGCT CGGGCTGCGG GGAGGGGAGC 
ATCAGCGTGG GGGAGAACGT GTCAGACGTG GCTCCCACCC AACCATGGTG GCCCAACACC 
CCGGGAGACA GTGTCTCTGA AGAACTAGGC CCTGGGAGGA GCGGGGACAC TGAGGACCTC 
TCTCCAAATT GGCCTCTCAA CTCACAGGAA GACACAGCTG CCCAGAGCAG CCCAGGCCGT 
GGTGAGGAGG CGGAGGCATC GGCGGCGGAG GCTCAGGGTG GGGAGCAGGC CTACCTGGCA 
GGCCTGGCAG GGCAGTACCA CTTGGAGCGG TACCCGGACA GTTACGAGTC CATGTCCGAG 
CCGCCCATTG CTCATCTTTT GCGCCCCGTG CTTCCCCGGG CCTTCGCCTT CCCCGTGGAC 
CCCCAGGTCC AGTCTGCCGC TGATGAGACT GCTGTGCAGC TGAGCGAGTT GCTGACGCTG 
CCCGTGCTCA TGAAGCGCTC CATCACGGCA CCGCTGGCCG CCCACATCTC CTTGGTGAAC 
AAGGCCGCTG TCGACTACTT CTTCGTGGAG CTGCACCTGG AGGCGCACTA TGAGGCACTG 
CGGCACTTCC TGCTGATGGA GGACGGCGAG TTCGCCCAGT CCCTCAGCGA CCTGCTCTTT 
GAGAAGCTTG GAGCTGGGCA AACGCCCGGA GAGCTGCTCA ACCCGCTGGT GCTGAACTCT 
GTGCTGAGCA AGGCCCTGCA GTGCAGCCTG CATGGGGACA CCCCGCACGC CTCCAACCTC 
TCCCTCGCTC TCAAGTACCT GCCCGAGGTG TTTGCCCCCA ACGCCCCGGA TGTGCTGAGC 
TGCCTGGAGC TCAGGTACAA GGTGGACTGG CCTCTCAACA TTGTCATCAC CGAGGGCTGC 
GTGAGCAAGT ACAGCGGCGT CTTCTCCTTC CTGCTGCAGC TGAAGCTCAT GATGTGGGCG 
CTCAAGGACG TCTGCTTCCA CCTCAAGCGC ACAGCCCTGC TGAGCCACAT GGCCGGCTCT 
GTGCAGTTCC GTCAGCTGCA GCTGTTCAAG CACGAGATGC AGCATTTCGT GAAGGTCATC 
CAGGGCTACA TCGCCAACCA GATCCTGCAC GTCACCTGGT GCGAGTTCAG GGCCAGGTTG 
GCCACCGTGG GCGACCTGGA GGAGATCCAG CGTGCGCACG CAGAGTACCT GCACAAGGCC 
GTCTTCAGGG GCCTGCTCAC GGAGAAGGCG GCGCCCGTCA TGAACGTCAT CCACAGCATC 
TTCAGCCTCG TGCTCAAGTT CCGCAGCCAG CTCATCTCCC AGGCCTGGGG GCCCCCTGGG 
GGCCCGCGGG GTGCAGAGCA CCCCAACTTT GCACTCATGC AGCAGTCCTA CAACACCTTC 
AAGTACTACT CCCACTTTCT CTTCAAAGTG GTGACCAAGC TGGTGAACCG CGGCTACCAG 
CCCCACCTGG AGGACTTTCT GCTGCGCATC AACTTCAACA ACTACTACCA GGACGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065194.3
CDS	565..6024
Translation	MASITQLFDDLCEALLPAAKTHLGQRSVNRKRAKRSLKKVAYNALFTNLFQDETQQLQPDMSKLPARNKILMLSFDLRVGGLGPKADRLEELVEELEAAPCCPLLEVGSVLDLLVQLAGSGPPQVLPRKRDYFLNNKHVGRNVPYSGYDCDDLSVFEMDVQSLISREECLCHSMIQETLQVMEAAPGTGLPTVGLFSFGDPCGDRFERDTRVSLFGALVHSRTYDMDVRLGLPPVPDNADLSGLAIKVPPSVDQWEDEGFQSASNLTPDSQSEPSVTPDVDLWEAALTYEASKRRCWERVGCPPGHREEPYLTEAGRDAFDKFCRLHQGELQLLAGGVLQAPQPVLVKECELVKDVLNVLIGVVSATFSLCQPAQAFVVKRGVHVSGASPESISSLLSEVAEYGTCYTRLSHFSLQPVLDSLYSKGLVFQAFTSGLRRYLQYYRACVLSTPPTLSLLTIGFLFKKLGRQLRYLAELCGVGAVLPGTCGGGPRAAFPTGVKLLSYLYQEALHNCSNEHYPVLLSLLKTSCEPYTRFIHDWVYSGVFRDAYGEFMIQVNHEYLSFRDKLYWTHGYVLISKEVEDCVPVFLKHIAHDIYVCGKTINLLKLCCPRHYLCWSDVPVPRISVIFSLEELKEIEKDCAVYVGRMERVARHSSVSKEEKELRMEIAKQELIAHAREAASRVLSALSDRQMSERMALDARKREQFQRLKEQFVKDQERRQAARQEELDDDFSYARELRDRERRLKSLEEELERKARQALVDHYSKLSAEAARREQKALWRIQRHRLESARLRFLLEDEKHIQEMLKAVSEAHQPQEPPDVLLSVHPQVTSPGPEHPEGGQGCDSGSAEQHSPAWDGWNRPGLLTPQPLKPLAVGAGGRGLQQAEGARPFSDSLSIGDFLPVGPGAEPSVQTGMVPLLEVALQTINLDLPPSAPGEAPAAASTQPSRPQEYDFSTVLRPAVATSPAPGPLQAAECSLGSSGLQLWEDSCGKMDACGSASRETLLPSHPPRRAALEEGSSQPTERLFGQVSGGGLPTGDYASEIAPTRPRWNTHGHVSDASIRVGENVSDVAPTQPRWNTHGHVSNASISLGESVSDVAPTRPRWNIHGHVSNASIRVGENVSDVAPTRPRWNTHGHVSNASIRVGENVSDVAPTRPRWNTHGHVSDASISLGESVSDMAPARPRWNTHGHVSDASISLGESVSDMAPTRPRWNTHGHVSDTSIRVGENVSDVAPIRSRCNTHGHVSDASISLGEPVSDVVSTRPRWNTHVPIPPPHMVLGALSPEAEPNTPRPQQSPPGHTSQSALSLGAQSTVLDCGPRLPVEVGPSLSSPSSGCGEGSISVGENVSDVAPTQPWWPNTPGDSVSEELGPGRSGDTEDLSPNWPLNSQEDTAAQSSPGRGEEAEASAAEAQGGEQAYLAGLAGQYHLERYPDSYESMSEPPIAHLLRPVLPRAFAFPVDPQVQSAADETAVQLSELLTLPVLMKRSITAPLAAHISLVNKAAVDYFFVELHLEAHYEALRHFLLMEDGEFAQSLSDLLFEKLGAGQTPGELLNPLVLNSVLSKALQCSLHGDTPHASNLSLALKYLPEVFAPNAPDVLSCLELRYKVDWPLNIVITEGCVSKYSGVFSFLLQLKLMMWALKDVCFHLKRTALLSHMAGSVQFRQLQLFKHEMQHFVKVIQGYIANQILHVTWCEFRARLATVGDLEEIQRAHAEYLHKAVFRGLLTEKAAPVMNVIHSIFSLVLKFRSQLISQAWGPPGGPRGAEHPNFALMQQSYNTFKYYSHFLFKVVTKLVNRGYQPHLEDFLLRINFNNYYQDA

Target ORF information:

RefSeq Version	NM_020461.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tubulin gamma complex associated protein 6 (TUBGCP6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020461.4

ORF Insert Sequence:

ATGGCCAGCA TCACGCAGCT GTTCGACGAC CTGTGTGAGG CCCTCCTGCC GGCTGCCAAG 
ACTCACCTGG GCCAGCGCAG TGTGAACCGG AAGAGGGCAA AGCGGAGCCT CAAGAAGGTG 
GCCTACAATG CTCTTTTCAC AAATCTTTTT CAAGATGAGA CTCAACAGCT GCAGCCTGAC 
ATGTCAAAAC TACCAGCGAG AAACAAGATC CTCATGTTGT CCTTTGACTT GAGAGTGGGT 
GGCCTGGGCC CCAAGGCCGA CCGTTTGGAG GAGCTTGTGG AGGAGCTGGA AGCAGCCCCT 
TGCTGTCCGC TTTTGGAGGT GGGGTCTGTT TTGGACCTCC TGGTTCAGCT GGCAGGGAGT 
GGTCCCCCTC AAGTTCTGCC GAGAAAACGA GACTACTTCC TTAACAACAA GCATGTGGGG 
AGAAACGTTC CGTACAGCGG CTATGATTGC GACGACCTGA GTGTGTTTGA GATGGACGTT 
CAGTCTCTGA TCTCCAGAGA AGAGTGTTTG TGTCACAGCA TGATCCAGGA AACACTTCAG 
GTTATGGAGG CTGCTCCAGG CACTGGCCTG CCCACCGTCG GGCTCTTCTC ATTTGGTGAC 
CCTTGTGGTG ACAGGTTCGA GAGAGACACC CGGGTCTCGC TCTTCGGGGC CCTTGTGCAC 
AGCCGCACTT ATGACATGGA CGTCCGACTG GGCCTGCCCC CCGTGCCAGA CAATGCGGAC 
CTCTCTGGGC TGGCTATTAA GGTCCCACCG AGTGTGGATC AGTGGGAAGA CGAAGGATTC 
CAGTCAGCGT CCAACTTGAC TCCTGATTCC CAGTCTGAGC CCAGCGTGAC CCCAGACGTG 
GACCTGTGGG AAGCCGCACT TACCTATGAG GCCAGCAAGC GGAGGTGCTG GGAGCGAGTT 
GGCTGCCCCC CTGGCCACAG AGAGGAGCCT TACCTGACGG AGGCGGGAAG GGACGCTTTC 
GACAAGTTCT GCAGGCTCCA CCAAGGGGAG CTCCAGCTGC TTGCTGGGGG CGTCCTACAG 
GCCCCGCAGC CCGTGCTGGT GAAGGAGTGC GAGCTGGTGA AAGACGTGCT GAACGTCTTG 
ATTGGGGTCG TGTCTGCCAC GTTTTCGCTC TGCCAGCCGG CCCAGGCCTT TGTGGTGAAG 
CGGGGCGTCC ACGTGTCAGG AGCGTCTCCC GAGAGCATCA GCAGCCTGCT CTCGGAAGTG 
GCCGAGTATG GGACCTGCTA CACGCGCCTG AGTCATTTCT CTCTGCAGCC CGTCCTGGAC 
TCTTTGTACA GCAAGGGCCT CGTGTTCCAG GCCTTCACCA GTGGCCTGAG GAGGTACCTG 
CAGTATTACC GGGCCTGCGT CCTTTCCACT CCGCCCACCC TGAGCCTCCT CACCATTGGT 
TTTCTCTTCA AGAAACTTGG CCGGCAGCTC AGGTACCTGG CCGAGCTCTG TGGCGTTGGC 
GCTGTGCTCC CGGGCACCTG TGGAGGAGGC CCCAGGGCCG CGTTTCCCAC CGGCGTGAAG 
CTGCTGTCCT ACCTCTACCA GGAGGCTCTG CACAACTGCA GCAACGAGCA CTACCCTGTA 
CTGCTGTCCC TGCTGAAGAC CAGCTGCGAG CCCTACACCC GGTTCATCCA CGACTGGGTG 
TACAGCGGGG TGTTCAGAGA CGCTTATGGC GAGTTCATGA TTCAGGTGAA CCACGAGTAC 
CTCAGCTTCC GAGATAAGTT GTACTGGACA CATGGCTACG TGCTCATCTC CAAAGAGGTG 
GAGGACTGTG TTCCCGTGTT TCTGAAGCAC ATTGCCCACG ACATATACGT CTGCGGAAAG 
ACCATTAACC TGCTGAAGCT CTGCTGCCCC CGGCATTACC TCTGTTGGTC CGACGTCCCT 
GTCCCCCGGA TCTCGGTGAT TTTCTCCCTT GAGGAGTTGA AGGAGATTGA GAAGGACTGT 
GCCGTCTACG TTGGGCGCAT GGAGAGGGTG GCCCGCCACA GCTCTGTCAG CAAGGAGGAG 
AAGGAATTAC GTATGGAAAT TGCAAAACAA GAATTAATCG CTCATGCCCG GGAAGCAGCA 
TCCAGGGTCC TGAGTGCACT GAGTGACCGG CAGATGTCAG AACGGATGGC CTTGGATGCC 
CGGAAGCGTG AGCAGTTTCA GAGGCTGAAA GAACAATTTG TGAAGGACCA GGAGCGACGC 
CAGGCGGCCA GGCAGGAGGA GCTGGATGAT GACTTCAGCT ACGCCCGTGA ACTCCGAGAC 
AGGGAGAGAA GGCTGAAGTC CCTGGAGGAG GAGCTGGAGA GGAAGGCGAG GCAGGCACTG 
GTCGACCACT ACAGCAAGCT CTCTGCAGAG GCAGCTCGTC GGGAGCAGAA GGCACTGTGG 
AGAATCCAGA GGCACCGACT GGAGAGTGCA CGGCTTCGTT TTCTCTTAGA AGATGAGAAA 
CACATTCAGG AGATGCTGAA AGCAGTGTCT GAGGCTCACC AGCCCCAGGA GCCGCCAGAC 
GTCCTCTTGA GCGTGCACCC CCAGGTCACG TCTCCGGGCC CTGAGCACCC AGAGGGAGGC 
CAAGGCTGTG ATTCTGGGTC TGCAGAGCAA CACTCGCCTG CCTGGGATGG CTGGAACAGG 
CCAGGCCTGC TGACCCCACA GCCCCTTAAG CCTCTAGCAG TGGGGGCTGG TGGCAGGGGG 
CTGCAGCAGG CGGAGGGGGC CAGACCCTTC TCTGACAGCC TCAGCATTGG AGACTTCCTA 
CCTGTGGGCC CAGGGGCTGA GCCGTCCGTG CAGACTGGCA TGGTCCCTCT CCTGGAGGTG 
GCGCTGCAGA CCATTAACTT GGACCTGCCC CCCTCAGCTC CTGGGGAGGC ACCCGCAGCA 
GCCAGCACTC AGCCCTCCAG GCCACAGGAG TACGACTTTA GTACTGTCCT GAGGCCAGCT 
GTGGCCACCT CACCTGCACC AGGGCCCCTG CAGGCTGCAG AGTGCAGCTT GGGCAGCTCA 
GGGCTACAGC TGTGGGAGGA CAGTTGTGGG AAGATGGATG CCTGTGGCTC CGCCTCGCGG 
GAGACTCTGC TCCCCTCACA CCCACCCAGG CGTGCTGCTC TGGAGGAGGG GAGCAGCCAG 
CCCACAGAGC GGCTCTTTGG GCAGGTGTCA GGGGGTGGTC TTCCCACAGG GGACTACGCT 
TCTGAAATAG CTCCCACCCG GCCACGGTGG AACACCCACG GGCACGTGTC TGACGCCAGC 
ATCAGGGTCG GGGAGAATGT GTCAGATGTG GCTCCCACCC AGCCACGGTG GAACACCCAC 
GGACACGTAT CCAATGCCAG CATCAGCTTA GGGGAGTCTG TGTCAGATGT GGCTCCCACT 
CGGCCACGGT GGAACATCCA TGGACATGTG TCCAACGCCA GCATCAGGGT CGGGGAGAAT 
GTGTCAGATG TGGCTCCCAC CAGGCCACGG TGGAATACCC ACGGGCACGT GTCCAACGCC 
AGCATCAGGG TTGGGGAGAA CGTGTCGGAC GTGGCTCCCA CCCGGCCACG GTGGAACACC 
CATGGACACG TGTCCGATGC CAGCATCAGC TTGGGGGAGT CTGTGTCAGA CATGGCTCCC 
GCCCGGCCAC GGTGGAACAC CCATGGACAC GTGTCTGATG CCAGCATCAG CTTGGGGGAG 
TCTGTGTCAG ACATGGCTCC CACCCGGCCA CGGTGGAACA CCCACGGACA TGTGTCTGAC 
ACCAGCATCA GGGTTGGGGA GAACGTATCG GACGTGGCTC CCATCCGGTC ACGGTGCAAC 
ACCCATGGAC ACGTGTCCGA CGCCAGCATC AGCTTGGGGG AGCCTGTGTC GGATGTGGTT 
TCCACCCGGC CACGGTGGAA CACCCATGTA CCCATCCCTC CGCCCCACAT GGTGCTGGGG 
GCTCTCTCAC CAGAAGCTGA GCCCAACACA CCCAGGCCCC AACAGAGCCC CCCTGGCCAC 
ACGTCCCAGT CAGCGCTCAG CCTGGGAGCA CAGAGCACTG TGCTGGACTG TGGGCCACGG 
CTGCCTGTAG AAGTGGGGCC ATCTCTGTCC TCCCCCAGCT CGGGCTGCGG GGAGGGGAGC 
ATCAGCGTGG GGGAGAACGT GTCAGACGTG GCTCCCACCC AACCATGGTG GCCCAACACC 
CCGGGAGACA GTGTCTCTGA AGAACTAGGC CCTGGGAGGA GCGGGGACAC TGAGGACCTC 
TCTCCAAATT GGCCTCTCAA CTCACAGGAA GACACAGCTG CCCAGAGCAG CCCAGGCCGT 
GGTGAGGAGG CGGAGGCATC GGCGGCGGAG GCTCAGGGTG GGGAGCAGGC CTACCTGGCA 
GGCCTGGCAG GGCAGTACCA CTTGGAGCGG TACCCGGACA GTTACGAGTC CATGTCCGAG 
CCGCCCATTG CTCATCTTTT GCGCCCCGTG CTTCCCCGGG CCTTCGCCTT CCCCGTGGAC 
CCCCAGGTCC AGTCTGCCGC TGATGAGACT GCTGTGCAGC TGAGCGAGTT GCTGACGCTG 
CCCGTGCTCA TGAAGCGCTC CATCACGGCA CCGCTGGCCG CCCACATCTC CTTGGTGAAC 
AAGGCCGCTG TCGACTACTT CTTCGTGGAG CTGCACCTGG AGGCGCACTA TGAGGCACTG 
CGGCACTTCC TGCTGATGGA GGACGGCGAG TTCGCCCAGT CCCTCAGCGA CCTGCTCTTT 
GAGAAGCTTG GAGCTGGGCA AACGCCCGGA GAGCTGCTCA ACCCGCTGGT GCTGAACTCT 
GTGCTGAGCA AGGCCCTGCA GTGCAGCCTG CATGGGGACA CCCCGCACGC CTCCAACCTC 
TCCCTCGCTC TCAAGTACCT GCCCGAGGTG TTTGCCCCCA ACGCCCCGGA TGTGCTGAGC 
TGCCTGGAGC TCAGGTACAA GGTGGACTGG CCTCTCAACA TTGTCATCAC CGAGGGCTGC 
GTGAGCAAGT ACAGCGGCGT CTTCTCCTTC CTGCTGCAGC TGAAGCTCAT GATGTGGGCG 
CTCAAGGACG TCTGCTTCCA CCTCAAGCGC ACAGCCCTGC TGAGCCACAT GGCCGGCTCT 
GTGCAGTTCC GTCAGCTGCA GCTGTTCAAG CACGAGATGC AGCATTTCGT GAAGGTCATC 
CAGGGCTACA TCGCCAACCA GATCCTGCAC GTCACCTGGT GCGAGTTCAG GGCCAGGTTG 
GCCACCGTGG GCGACCTGGA GGAGATCCAG CGTGCGCACG CAGAGTACCT GCACAAGGCC 
GTCTTCAGGG GCCTGCTCAC GGAGAAGGCG GCGCCCGTCA TGAACGTCAT CCACAGCATC 
TTCAGCCTCG TGCTCAAGTT CCGCAGCCAG CTCATCTCCC AGGCCTGGGG GCCCCCTGGG 
GGCCCGCGGG GTGCAGAGCA CCCCAACTTT GCACTCATGC AGCAGTCCTA CAACACCTTC 
AAGTACTACT CCCACTTTCT CTTCAAAGTG GTGACCAAGC TGGTGAACCG CGGCTACCAG 
CCCCACCTGG AGGACTTTCT GCTGCGCATC AACTTCAACA ACTACTACCA GGACGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TUBGCP6 ( NM_020461.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065194.2 Homo sapiens tubulin gamma complex associated protein 6 (TUBGCP6), mRNA.

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CloneID	OHu17827
Clone ID Related Accession (Same CDS sequence)	NM_020461.3
Accession Version	NM_020461.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	gamma-tubulin complex component 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832617.2, CN333235.1, AF272887.1, AB051456.1, BC042165.1 and BC023573.2. This sequence is a reference standard in the RefSeqGene project. On Dec 22, 2004 this sequence version replaced NM_020461.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF272887.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGCA TCACGCAGCT GTTCGACGAC CTGTGTGAGG CCCTCCTGCC GGCTGCCAAG 
ACTCACCTGG GCCAGCGCAG TGTGAACCGG AAGAGGGCAA AGCGGAGCCT CAAGAAGGTG 
GCCTACAATG CTCTTTTCAC AAATCTTTTT CAAGATGAGA CTCAACAGCT GCAGCCTGAC 
ATGTCAAAAC TACCAGCGAG AAACAAGATC CTCATGTTGT CCTTTGACTT GAGAGTGGGT 
GGCCTGGGCC CCAAGGCCGA CCGTTTGGAG GAGCTTGTGG AGGAGCTGGA AGCAGCCCCT 
TGCTGTCCGC TTTTGGAGGT GGGGTCTGTT TTGGACCTCC TGGTTCAGCT GGCAGGGAGT 
GGTCCCCCTC AAGTTCTGCC GAGAAAACGA GACTACTTCC TTAACAACAA GCATGTGGGG 
AGAAACGTTC CGTACAGCGG CTATGATTGC GACGACCTGA GTGTGTTTGA GATGGACGTT 
CAGTCTCTGA TCTCCAGAGA AGAGTGTTTG TGTCACAGCA TGATCCAGGA AACACTTCAG 
GTTATGGAGG CTGCTCCAGG CACTGGCCTG CCCACCGTCG GGCTCTTCTC ATTTGGTGAC 
CCTTGTGGTG ACAGGTTCGA GAGAGACACC CGGGTCTCGC TCTTCGGGGC CCTTGTGCAC 
AGCCGCACTT ATGACATGGA CGTCCGACTG GGCCTGCCCC CCGTGCCAGA CAATGCGGAC 
CTCTCTGGGC TGGCTATTAA GGTCCCACCG AGTGTGGATC AGTGGGAAGA CGAAGGATTC 
CAGTCAGCGT CCAACTTGAC TCCTGATTCC CAGTCTGAGC CCAGCGTGAC CCCAGACGTG 
GACCTGTGGG AAGCCGCACT TACCTATGAG GCCAGCAAGC GGAGGTGCTG GGAGCGAGTT 
GGCTGCCCCC CTGGCCACAG AGAGGAGCCT TACCTGACGG AGGCGGGAAG GGACGCTTTC 
GACAAGTTCT GCAGGCTCCA CCAAGGGGAG CTCCAGCTGC TTGCTGGGGG CGTCCTACAG 
GCCCCGCAGC CCGTGCTGGT GAAGGAGTGC GAGCTGGTGA AAGACGTGCT GAACGTCTTG 
ATTGGGGTCG TGTCTGCCAC GTTTTCGCTC TGCCAGCCGG CCCAGGCCTT TGTGGTGAAG 
CGGGGCGTCC ACGTGTCAGG AGCGTCTCCC GAGAGCATCA GCAGCCTGCT CTCGGAAGTG 
GCCGAGTATG GGACCTGCTA CACGCGCCTG AGTCATTTCT CTCTGCAGCC CGTCCTGGAC 
TCTTTGTACA GCAAGGGCCT CGTGTTCCAG GCCTTCACCA GTGGCCTGAG GAGGTACCTG 
CAGTATTACC GGGCCTGCGT CCTTTCCACT CCGCCCACCC TGAGCCTCCT CACCATTGGT 
TTTCTCTTCA AGAAACTTGG CCGGCAGCTC AGGTACCTGG CCGAGCTCTG TGGCGTTGGC 
GCTGTGCTCC CGGGCACCTG TGGAGGAGGC CCCAGGGCCG CGTTTCCCAC CGGCGTGAAG 
CTGCTGTCCT ACCTCTACCA GGAGGCTCTG CACAACTGCA GCAACGAGCA CTACCCTGTA 
CTGCTGTCCC TGCTGAAGAC CAGCTGCGAG CCCTACACCC GGTTCATCCA CGACTGGGTG 
TACAGCGGGG TGTTCAGAGA CGCTTATGGC GAGTTCATGA TTCAGGTGAA CCACGAGTAC 
CTCAGCTTCC GAGATAAGTT GTACTGGACA CATGGCTACG TGCTCATCTC CAAAGAGGTG 
GAGGACTGTG TTCCCGTGTT TCTGAAGCAC ATTGCCCACG ACATATACGT CTGCGGAAAG 
ACCATTAACC TGCTGAAGCT CTGCTGCCCC CGGCATTACC TCTGTTGGTC CGACGTCCCT 
GTCCCCCGGA TCTCGGTGAT TTTCTCCCTT GAGGAGTTGA AGGAGATTGA GAAGGACTGT 
GCCGTCTACG TTGGGCGCAT GGAGAGGGTG GCCCGCCACA GCTCTGTCAG CAAGGAGGAG 
AAGGAATTAC GTATGGAAAT TGCAAAACAA GAATTAATCG CTCATGCCCG GGAAGCAGCA 
TCCAGGGTCC TGAGTGCACT GAGTGACCGG CAGATGTCAG AACGGATGGC CTTGGATGCC 
CGGAAGCGTG AGCAGTTTCA GAGGCTGAAA GAACAATTTG TGAAGGACCA GGAGCGACGC 
CAGGCGGCCA GGCAGGAGGA GCTGGATGAT GACTTCAGCT ACGCCCGTGA ACTCCGAGAC 
AGGGAGAGAA GGCTGAAGTC CCTGGAGGAG GAGCTGGAGA GGAAGGCGAG GCAGGCACTG 
GTCGACCACT ACAGCAAGCT CTCTGCAGAG GCAGCTCGTC GGGAGCAGAA GGCACTGTGG 
AGAATCCAGA GGCACCGACT GGAGAGTGCA CGGCTTCGTT TTCTCTTAGA AGATGAGAAA 
CACATTCAGG AGATGCTGAA AGCAGTGTCT GAGGCTCACC AGCCCCAGGA GCCGCCAGAC 
GTCCTCTTGA GCGTGCACCC CCAGGTCACG TCTCCGGGCC CTGAGCACCC AGAGGGAGGC 
CAAGGCTGTG ATTCTGGGTC TGCAGAGCAA CACTCGCCTG CCTGGGATGG CTGGAACAGG 
CCAGGCCTGC TGACCCCACA GCCCCTTAAG CCTCTAGCAG TGGGGGCTGG TGGCAGGGGG 
CTGCAGCAGG CGGAGGGGGC CAGACCCTTC TCTGACAGCC TCAGCATTGG AGACTTCCTA 
CCTGTGGGCC CAGGGGCTGA GCCGTCCGTG CAGACTGGCA TGGTCCCTCT CCTGGAGGTG 
GCGCTGCAGA CCATTAACTT GGACCTGCCC CCCTCAGCTC CTGGGGAGGC ACCCGCAGCA 
GCCAGCACTC AGCCCTCCAG GCCACAGGAG TACGACTTTA GTACTGTCCT GAGGCCAGCT 
GTGGCCACCT CACCTGCACC AGGGCCCCTG CAGGCTGCAG AGTGCAGCTT GGGCAGCTCA 
GGGCTACAGC TGTGGGAGGA CAGTTGTGGG AAGATGGATG CCTGTGGCTC CGCCTCGCGG 
GAGACTCTGC TCCCCTCACA CCCACCCAGG CGTGCTGCTC TGGAGGAGGG GAGCAGCCAG 
CCCACAGAGC GGCTCTTTGG GCAGGTGTCA GGGGGTGGTC TTCCCACAGG GGACTACGCT 
TCTGAAATAG CTCCCACCCG GCCACGGTGG AACACCCACG GGCACGTGTC TGACGCCAGC 
ATCAGGGTCG GGGAGAATGT GTCAGATGTG GCTCCCACCC AGCCACGGTG GAACACCCAC 
GGACACGTAT CCAATGCCAG CATCAGCTTA GGGGAGTCTG TGTCAGATGT GGCTCCCACT 
CGGCCACGGT GGAACATCCA TGGACATGTG TCCAACGCCA GCATCAGGGT CGGGGAGAAT 
GTGTCAGATG TGGCTCCCAC CAGGCCACGG TGGAATACCC ACGGGCACGT GTCCAACGCC 
AGCATCAGGG TTGGGGAGAA CGTGTCGGAC GTGGCTCCCA CCCGGCCACG GTGGAACACC 
CATGGACACG TGTCCGATGC CAGCATCAGC TTGGGGGAGT CTGTGTCAGA CATGGCTCCC 
GCCCGGCCAC GGTGGAACAC CCATGGACAC GTGTCTGATG CCAGCATCAG CTTGGGGGAG 
TCTGTGTCAG ACATGGCTCC CACCCGGCCA CGGTGGAACA CCCACGGACA TGTGTCTGAC 
ACCAGCATCA GGGTTGGGGA GAACGTATCG GACGTGGCTC CCATCCGGTC ACGGTGCAAC 
ACCCATGGAC ACGTGTCCGA CGCCAGCATC AGCTTGGGGG AGCCTGTGTC GGATGTGGTT 
TCCACCCGGC CACGGTGGAA CACCCATGTA CCCATCCCTC CGCCCCACAT GGTGCTGGGG 
GCTCTCTCAC CAGAAGCTGA GCCCAACACA CCCAGGCCCC AACAGAGCCC CCCTGGCCAC 
ACGTCCCAGT CAGCGCTCAG CCTGGGAGCA CAGAGCACTG TGCTGGACTG TGGGCCACGG 
CTGCCTGTAG AAGTGGGGCC ATCTCTGTCC TCCCCCAGCT CGGGCTGCGG GGAGGGGAGC 
ATCAGCGTGG GGGAGAACGT GTCAGACGTG GCTCCCACCC AACCATGGTG GCCCAACACC 
CCGGGAGACA GTGTCTCTGA AGAACTAGGC CCTGGGAGGA GCGGGGACAC TGAGGACCTC 
TCTCCAAATT GGCCTCTCAA CTCACAGGAA GACACAGCTG CCCAGAGCAG CCCAGGCCGT 
GGTGAGGAGG CGGAGGCATC GGCGGCGGAG GCTCAGGGTG GGGAGCAGGC CTACCTGGCA 
GGCCTGGCAG GGCAGTACCA CTTGGAGCGG TACCCGGACA GTTACGAGTC CATGTCCGAG 
CCGCCCATTG CTCATCTTTT GCGCCCCGTG CTTCCCCGGG CCTTCGCCTT CCCCGTGGAC 
CCCCAGGTCC AGTCTGCCGC TGATGAGACT GCTGTGCAGC TGAGCGAGTT GCTGACGCTG 
CCCGTGCTCA TGAAGCGCTC CATCACGGCA CCGCTGGCCG CCCACATCTC CTTGGTGAAC 
AAGGCCGCTG TCGACTACTT CTTCGTGGAG CTGCACCTGG AGGCGCACTA TGAGGCACTG 
CGGCACTTCC TGCTGATGGA GGACGGCGAG TTCGCCCAGT CCCTCAGCGA CCTGCTCTTT 
GAGAAGCTTG GAGCTGGGCA AACGCCCGGA GAGCTGCTCA ACCCGCTGGT GCTGAACTCT 
GTGCTGAGCA AGGCCCTGCA GTGCAGCCTG CATGGGGACA CCCCGCACGC CTCCAACCTC 
TCCCTCGCTC TCAAGTACCT GCCCGAGGTG TTTGCCCCCA ACGCCCCGGA TGTGCTGAGC 
TGCCTGGAGC TCAGGTACAA GGTGGACTGG CCTCTCAACA TTGTCATCAC CGAGGGCTGC 
CTGAGCAAGT ACAGCGGCGT CTTCTCCTTC CTGCTGCAGC TGAAGCTCAT GATGTGGGCG 
CTCAAGGACG TCTGCTTCCA CCTCAAGCGC ACAGCCCTGC TGAGCCACAT GGCCGGCTCT 
GTGCAGTTCC GTCAGCTGCA GCTGTTCAAG CACGAGATGC AGCATTTCGT GAAGGTCATC 
CAGGGCTACA TCGCCAACCA GATCCTGCAC GTCACCTGGT GCGAGTTCAG GGCCAGGTTG 
GCCACCGTGG GCGACCTGGA GGAGATCCAG CGTGCGCACG CAGAGTACCT GCACAAGGCC 
GTCTTCAGGG GCCTGCTCAC GGAGAAGGCG GCGCCCGTCA TGAACGTCAT CCACAGCATC 
TTCAGCCTCG TGCTCAAGTT CCGCAGCCAG CTCATCTCCC AGGCCTGGGG GCCCCCTGGG 
GGCCCGCGGG GTGCAGAGCA CCCCAACTTT GCACTCATGC AGCAGTCCTA CAACACCTTC 
AAGTACTACT CCCACTTTCT CTTCAAAGTG GTGACCAAGC TGGTGAACCG CGGCTACCAG 
CCCCACCTGG AGGACTTTCT GCTGCGCATC AACTTCAACA ACTACTACCA GGACGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065194.2
CDS	513..5972
Translation	MASITQLFDDLCEALLPAAKTHLGQRSVNRKRAKRSLKKVAYNALFTNLFQDETQQLQPDMSKLPARNKILMLSFDLRVGGLGPKADRLEELVEELEAAPCCPLLEVGSVLDLLVQLAGSGPPQVLPRKRDYFLNNKHVGRNVPYSGYDCDDLSVFEMDVQSLISREECLCHSMIQETLQVMEAAPGTGLPTVGLFSFGDPCGDRFERDTRVSLFGALVHSRTYDMDVRLGLPPVPDNADLSGLAIKVPPSVDQWEDEGFQSASNLTPDSQSEPSVTPDVDLWEAALTYEASKRRCWERVGCPPGHREEPYLTEAGRDAFDKFCRLHQGELQLLAGGVLQAPQPVLVKECELVKDVLNVLIGVVSATFSLCQPAQAFVVKRGVHVSGASPESISSLLSEVAEYGTCYTRLSHFSLQPVLDSLYSKGLVFQAFTSGLRRYLQYYRACVLSTPPTLSLLTIGFLFKKLGRQLRYLAELCGVGAVLPGTCGGGPRAAFPTGVKLLSYLYQEALHNCSNEHYPVLLSLLKTSCEPYTRFIHDWVYSGVFRDAYGEFMIQVNHEYLSFRDKLYWTHGYVLISKEVEDCVPVFLKHIAHDIYVCGKTINLLKLCCPRHYLCWSDVPVPRISVIFSLEELKEIEKDCAVYVGRMERVARHSSVSKEEKELRMEIAKQELIAHAREAASRVLSALSDRQMSERMALDARKREQFQRLKEQFVKDQERRQAARQEELDDDFSYARELRDRERRLKSLEEELERKARQALVDHYSKLSAEAARREQKALWRIQRHRLESARLRFLLEDEKHIQEMLKAVSEAHQPQEPPDVLLSVHPQVTSPGPEHPEGGQGCDSGSAEQHSPAWDGWNRPGLLTPQPLKPLAVGAGGRGLQQAEGARPFSDSLSIGDFLPVGPGAEPSVQTGMVPLLEVALQTINLDLPPSAPGEAPAAASTQPSRPQEYDFSTVLRPAVATSPAPGPLQAAECSLGSSGLQLWEDSCGKMDACGSASRETLLPSHPPRRAALEEGSSQPTERLFGQVSGGGLPTGDYASEIAPTRPRWNTHGHVSDASIRVGENVSDVAPTQPRWNTHGHVSNASISLGESVSDVAPTRPRWNIHGHVSNASIRVGENVSDVAPTRPRWNTHGHVSNASIRVGENVSDVAPTRPRWNTHGHVSDASISLGESVSDMAPARPRWNTHGHVSDASISLGESVSDMAPTRPRWNTHGHVSDTSIRVGENVSDVAPIRSRCNTHGHVSDASISLGEPVSDVVSTRPRWNTHVPIPPPHMVLGALSPEAEPNTPRPQQSPPGHTSQSALSLGAQSTVLDCGPRLPVEVGPSLSSPSSGCGEGSISVGENVSDVAPTQPWWPNTPGDSVSEELGPGRSGDTEDLSPNWPLNSQEDTAAQSSPGRGEEAEASAAEAQGGEQAYLAGLAGQYHLERYPDSYESMSEPPIAHLLRPVLPRAFAFPVDPQVQSAADETAVQLSELLTLPVLMKRSITAPLAAHISLVNKAAVDYFFVELHLEAHYEALRHFLLMEDGEFAQSLSDLLFEKLGAGQTPGELLNPLVLNSVLSKALQCSLHGDTPHASNLSLALKYLPEVFAPNAPDVLSCLELRYKVDWPLNIVITEGCLSKYSGVFSFLLQLKLMMWALKDVCFHLKRTALLSHMAGSVQFRQLQLFKHEMQHFVKVIQGYIANQILHVTWCEFRARLATVGDLEEIQRAHAEYLHKAVFRGLLTEKAAPVMNVIHSIFSLVLKFRSQLISQAWGPPGGPRGAEHPNFALMQQSYNTFKYYSHFLFKVVTKLVNRGYQPHLEDFLLRINFNNYYQDA

Target ORF information:

RefSeq Version	NM_020461.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tubulin gamma complex associated protein 6 (TUBGCP6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020461.3

ORF Insert Sequence:

ATGGCCAGCA TCACGCAGCT GTTCGACGAC CTGTGTGAGG CCCTCCTGCC GGCTGCCAAG 
ACTCACCTGG GCCAGCGCAG TGTGAACCGG AAGAGGGCAA AGCGGAGCCT CAAGAAGGTG 
GCCTACAATG CTCTTTTCAC AAATCTTTTT CAAGATGAGA CTCAACAGCT GCAGCCTGAC 
ATGTCAAAAC TACCAGCGAG AAACAAGATC CTCATGTTGT CCTTTGACTT GAGAGTGGGT 
GGCCTGGGCC CCAAGGCCGA CCGTTTGGAG GAGCTTGTGG AGGAGCTGGA AGCAGCCCCT 
TGCTGTCCGC TTTTGGAGGT GGGGTCTGTT TTGGACCTCC TGGTTCAGCT GGCAGGGAGT 
GGTCCCCCTC AAGTTCTGCC GAGAAAACGA GACTACTTCC TTAACAACAA GCATGTGGGG 
AGAAACGTTC CGTACAGCGG CTATGATTGC GACGACCTGA GTGTGTTTGA GATGGACGTT 
CAGTCTCTGA TCTCCAGAGA AGAGTGTTTG TGTCACAGCA TGATCCAGGA AACACTTCAG 
GTTATGGAGG CTGCTCCAGG CACTGGCCTG CCCACCGTCG GGCTCTTCTC ATTTGGTGAC 
CCTTGTGGTG ACAGGTTCGA GAGAGACACC CGGGTCTCGC TCTTCGGGGC CCTTGTGCAC 
AGCCGCACTT ATGACATGGA CGTCCGACTG GGCCTGCCCC CCGTGCCAGA CAATGCGGAC 
CTCTCTGGGC TGGCTATTAA GGTCCCACCG AGTGTGGATC AGTGGGAAGA CGAAGGATTC 
CAGTCAGCGT CCAACTTGAC TCCTGATTCC CAGTCTGAGC CCAGCGTGAC CCCAGACGTG 
GACCTGTGGG AAGCCGCACT TACCTATGAG GCCAGCAAGC GGAGGTGCTG GGAGCGAGTT 
GGCTGCCCCC CTGGCCACAG AGAGGAGCCT TACCTGACGG AGGCGGGAAG GGACGCTTTC 
GACAAGTTCT GCAGGCTCCA CCAAGGGGAG CTCCAGCTGC TTGCTGGGGG CGTCCTACAG 
GCCCCGCAGC CCGTGCTGGT GAAGGAGTGC GAGCTGGTGA AAGACGTGCT GAACGTCTTG 
ATTGGGGTCG TGTCTGCCAC GTTTTCGCTC TGCCAGCCGG CCCAGGCCTT TGTGGTGAAG 
CGGGGCGTCC ACGTGTCAGG AGCGTCTCCC GAGAGCATCA GCAGCCTGCT CTCGGAAGTG 
GCCGAGTATG GGACCTGCTA CACGCGCCTG AGTCATTTCT CTCTGCAGCC CGTCCTGGAC 
TCTTTGTACA GCAAGGGCCT CGTGTTCCAG GCCTTCACCA GTGGCCTGAG GAGGTACCTG 
CAGTATTACC GGGCCTGCGT CCTTTCCACT CCGCCCACCC TGAGCCTCCT CACCATTGGT 
TTTCTCTTCA AGAAACTTGG CCGGCAGCTC AGGTACCTGG CCGAGCTCTG TGGCGTTGGC 
GCTGTGCTCC CGGGCACCTG TGGAGGAGGC CCCAGGGCCG CGTTTCCCAC CGGCGTGAAG 
CTGCTGTCCT ACCTCTACCA GGAGGCTCTG CACAACTGCA GCAACGAGCA CTACCCTGTA 
CTGCTGTCCC TGCTGAAGAC CAGCTGCGAG CCCTACACCC GGTTCATCCA CGACTGGGTG 
TACAGCGGGG TGTTCAGAGA CGCTTATGGC GAGTTCATGA TTCAGGTGAA CCACGAGTAC 
CTCAGCTTCC GAGATAAGTT GTACTGGACA CATGGCTACG TGCTCATCTC CAAAGAGGTG 
GAGGACTGTG TTCCCGTGTT TCTGAAGCAC ATTGCCCACG ACATATACGT CTGCGGAAAG 
ACCATTAACC TGCTGAAGCT CTGCTGCCCC CGGCATTACC TCTGTTGGTC CGACGTCCCT 
GTCCCCCGGA TCTCGGTGAT TTTCTCCCTT GAGGAGTTGA AGGAGATTGA GAAGGACTGT 
GCCGTCTACG TTGGGCGCAT GGAGAGGGTG GCCCGCCACA GCTCTGTCAG CAAGGAGGAG 
AAGGAATTAC GTATGGAAAT TGCAAAACAA GAATTAATCG CTCATGCCCG GGAAGCAGCA 
TCCAGGGTCC TGAGTGCACT GAGTGACCGG CAGATGTCAG AACGGATGGC CTTGGATGCC 
CGGAAGCGTG AGCAGTTTCA GAGGCTGAAA GAACAATTTG TGAAGGACCA GGAGCGACGC 
CAGGCGGCCA GGCAGGAGGA GCTGGATGAT GACTTCAGCT ACGCCCGTGA ACTCCGAGAC 
AGGGAGAGAA GGCTGAAGTC CCTGGAGGAG GAGCTGGAGA GGAAGGCGAG GCAGGCACTG 
GTCGACCACT ACAGCAAGCT CTCTGCAGAG GCAGCTCGTC GGGAGCAGAA GGCACTGTGG 
AGAATCCAGA GGCACCGACT GGAGAGTGCA CGGCTTCGTT TTCTCTTAGA AGATGAGAAA 
CACATTCAGG AGATGCTGAA AGCAGTGTCT GAGGCTCACC AGCCCCAGGA GCCGCCAGAC 
GTCCTCTTGA GCGTGCACCC CCAGGTCACG TCTCCGGGCC CTGAGCACCC AGAGGGAGGC 
CAAGGCTGTG ATTCTGGGTC TGCAGAGCAA CACTCGCCTG CCTGGGATGG CTGGAACAGG 
CCAGGCCTGC TGACCCCACA GCCCCTTAAG CCTCTAGCAG TGGGGGCTGG TGGCAGGGGG 
CTGCAGCAGG CGGAGGGGGC CAGACCCTTC TCTGACAGCC TCAGCATTGG AGACTTCCTA 
CCTGTGGGCC CAGGGGCTGA GCCGTCCGTG CAGACTGGCA TGGTCCCTCT CCTGGAGGTG 
GCGCTGCAGA CCATTAACTT GGACCTGCCC CCCTCAGCTC CTGGGGAGGC ACCCGCAGCA 
GCCAGCACTC AGCCCTCCAG GCCACAGGAG TACGACTTTA GTACTGTCCT GAGGCCAGCT 
GTGGCCACCT CACCTGCACC AGGGCCCCTG CAGGCTGCAG AGTGCAGCTT GGGCAGCTCA 
GGGCTACAGC TGTGGGAGGA CAGTTGTGGG AAGATGGATG CCTGTGGCTC CGCCTCGCGG 
GAGACTCTGC TCCCCTCACA CCCACCCAGG CGTGCTGCTC TGGAGGAGGG GAGCAGCCAG 
CCCACAGAGC GGCTCTTTGG GCAGGTGTCA GGGGGTGGTC TTCCCACAGG GGACTACGCT 
TCTGAAATAG CTCCCACCCG GCCACGGTGG AACACCCACG GGCACGTGTC TGACGCCAGC 
ATCAGGGTCG GGGAGAATGT GTCAGATGTG GCTCCCACCC AGCCACGGTG GAACACCCAC 
GGACACGTAT CCAATGCCAG CATCAGCTTA GGGGAGTCTG TGTCAGATGT GGCTCCCACT 
CGGCCACGGT GGAACATCCA TGGACATGTG TCCAACGCCA GCATCAGGGT CGGGGAGAAT 
GTGTCAGATG TGGCTCCCAC CAGGCCACGG TGGAATACCC ACGGGCACGT GTCCAACGCC 
AGCATCAGGG TTGGGGAGAA CGTGTCGGAC GTGGCTCCCA CCCGGCCACG GTGGAACACC 
CATGGACACG TGTCCGATGC CAGCATCAGC TTGGGGGAGT CTGTGTCAGA CATGGCTCCC 
GCCCGGCCAC GGTGGAACAC CCATGGACAC GTGTCTGATG CCAGCATCAG CTTGGGGGAG 
TCTGTGTCAG ACATGGCTCC CACCCGGCCA CGGTGGAACA CCCACGGACA TGTGTCTGAC 
ACCAGCATCA GGGTTGGGGA GAACGTATCG GACGTGGCTC CCATCCGGTC ACGGTGCAAC 
ACCCATGGAC ACGTGTCCGA CGCCAGCATC AGCTTGGGGG AGCCTGTGTC GGATGTGGTT 
TCCACCCGGC CACGGTGGAA CACCCATGTA CCCATCCCTC CGCCCCACAT GGTGCTGGGG 
GCTCTCTCAC CAGAAGCTGA GCCCAACACA CCCAGGCCCC AACAGAGCCC CCCTGGCCAC 
ACGTCCCAGT CAGCGCTCAG CCTGGGAGCA CAGAGCACTG TGCTGGACTG TGGGCCACGG 
CTGCCTGTAG AAGTGGGGCC ATCTCTGTCC TCCCCCAGCT CGGGCTGCGG GGAGGGGAGC 
ATCAGCGTGG GGGAGAACGT GTCAGACGTG GCTCCCACCC AACCATGGTG GCCCAACACC 
CCGGGAGACA GTGTCTCTGA AGAACTAGGC CCTGGGAGGA GCGGGGACAC TGAGGACCTC 
TCTCCAAATT GGCCTCTCAA CTCACAGGAA GACACAGCTG CCCAGAGCAG CCCAGGCCGT 
GGTGAGGAGG CGGAGGCATC GGCGGCGGAG GCTCAGGGTG GGGAGCAGGC CTACCTGGCA 
GGCCTGGCAG GGCAGTACCA CTTGGAGCGG TACCCGGACA GTTACGAGTC CATGTCCGAG 
CCGCCCATTG CTCATCTTTT GCGCCCCGTG CTTCCCCGGG CCTTCGCCTT CCCCGTGGAC 
CCCCAGGTCC AGTCTGCCGC TGATGAGACT GCTGTGCAGC TGAGCGAGTT GCTGACGCTG 
CCCGTGCTCA TGAAGCGCTC CATCACGGCA CCGCTGGCCG CCCACATCTC CTTGGTGAAC 
AAGGCCGCTG TCGACTACTT CTTCGTGGAG CTGCACCTGG AGGCGCACTA TGAGGCACTG 
CGGCACTTCC TGCTGATGGA GGACGGCGAG TTCGCCCAGT CCCTCAGCGA CCTGCTCTTT 
GAGAAGCTTG GAGCTGGGCA AACGCCCGGA GAGCTGCTCA ACCCGCTGGT GCTGAACTCT 
GTGCTGAGCA AGGCCCTGCA GTGCAGCCTG CATGGGGACA CCCCGCACGC CTCCAACCTC 
TCCCTCGCTC TCAAGTACCT GCCCGAGGTG TTTGCCCCCA ACGCCCCGGA TGTGCTGAGC 
TGCCTGGAGC TCAGGTACAA GGTGGACTGG CCTCTCAACA TTGTCATCAC CGAGGGCTGC 
CTGAGCAAGT ACAGCGGCGT CTTCTCCTTC CTGCTGCAGC TGAAGCTCAT GATGTGGGCG 
CTCAAGGACG TCTGCTTCCA CCTCAAGCGC ACAGCCCTGC TGAGCCACAT GGCCGGCTCT 
GTGCAGTTCC GTCAGCTGCA GCTGTTCAAG CACGAGATGC AGCATTTCGT GAAGGTCATC 
CAGGGCTACA TCGCCAACCA GATCCTGCAC GTCACCTGGT GCGAGTTCAG GGCCAGGTTG 
GCCACCGTGG GCGACCTGGA GGAGATCCAG CGTGCGCACG CAGAGTACCT GCACAAGGCC 
GTCTTCAGGG GCCTGCTCAC GGAGAAGGCG GCGCCCGTCA TGAACGTCAT CCACAGCATC 
TTCAGCCTCG TGCTCAAGTT CCGCAGCCAG CTCATCTCCC AGGCCTGGGG GCCCCCTGGG 
GGCCCGCGGG GTGCAGAGCA CCCCAACTTT GCACTCATGC AGCAGTCCTA CAACACCTTC 
AAGTACTACT CCCACTTTCT CTTCAAAGTG GTGACCAAGC TGGTGAACCG CGGCTACCAG 
CCCCACCTGG AGGACTTTCT GCTGCGCATC AACTTCAACA ACTACTACCA GGACGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Nature communications54020(2014 Jun)
Navarini AA,Simpson MA,Weale M,Knight J,Carlavan I,Reiniche P,Burden DA,Layton A,Bataille V,Allen M,Pleass R,Pink A,Creamer D,English J,Munn S,Walton S,,Willis C,D?ret S,Voegel JJ,Spector T,Smith CH,Trembath RC,Barker JN

Gene network analysis in a pediatric cohort identifies novel lung function genes.
PloS one8(9)e72899(2013)
Ong BA,Li J,McDonough JM,Wei Z,Kim C,Chiavacci R,Mentch F,Caboot JB,Spergel J,Allen JL,Sleiman PM,Hakonarson H

GCP6 is a substrate of Plk4 and required for centriole duplication.
Journal of cell science125(Pt 2)486-96(2012 Jan)
Bahtz R,Seidler J,Arnold M,Haselmann-Weiss U,Antony C,Lehmann WD,Hoffmann I

Genetic mapping and exome sequencing identify variants associated with five novel diseases.
PloS one7(1)e28936(2012)
Puffenberger EG,Jinks RN,Sougnez C,Cibulskis K,Willert RA,Achilly NP,Cassidy RP,Fiorentini CJ,Heiken KF,Lawrence JJ,Mahoney MH,Miller CJ,Nair DT,Politi KA,Worcester KN,Setton RA,Dipiazza R,Sherman EA,Eastman JT,Francklyn C,Robey-Bond S,Rider NL,Gabriel S,Morton DH,Strauss KA

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF

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TUBGCP6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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