MYLK2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYLK2
Entrez Gene ID	85366
Full Name	myosin light chain kinase 2
Synonyms	KMLC,MLCK,MLCK2,skMLCK
General protein information	Preferred Names myosin light chain kinase 2 Names myosin light chain kinase 2, skeletal/cardiac muscle myosin light chain kinase 2, skeletal muscle
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q11.21
Summary	This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle. [provided by RefSeq, Jul 2008].
Disorder MIM:	606566
Disorder Html:	Cardiomyopathy, hypertrophic, 1, digenic, 192600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_033118.4	NP_149109.1	myosin light chain kinase 2, skeletal/cardiac muscle
NM_033118.3	NP_149109.1	myosin light chain kinase 2, skeletal/cardiac muscle

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MYLK2	XP_005634973.1
Drosophila melanogaster (fruit fly)	sqa	NP_610514.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006746	XP_308996.3
Bos taurus (cattle)	MYLK2	NP_001077188.1
Rattus norvegicus (Norway rat)	Mylk2	NP_476557.1
	LOC712830	XP_002798235.1
Gallus gallus (chicken)	MYLK2	NP_990723.1
Homo sapiens (human)	MYLK2	NP_149109.1
Pan troglodytes (chimpanzee)	MYLK2	XP_003316951.1
Mus musculus (house mouse)	Mylk2	NP_001074513.2

Gene Ontology
Bibliography

Related articles in PubMed

Dual LQT1 and HCM phenotypes associated with tetrad heterozygous mutations in KCNQ1, MYH7, MYLK2, and TMEM70 genes in a three-generation Chinese family.
Wang L, Zuo L, Hu J, Shao H, Lei C, Qi W, Liu Y, Miao Y, Ma X, Huang CL, Wang B, Zhou X, Zhang Y, Liu L
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology18(4)602-9(2016 Apr)

Skeletal myosin light chain kinase regulates skeletal myogenesis by phosphorylation of MEF2C.
Al Madhoun AS, Mehta V, Li G, Figeys D, Wiper-Bergeron N, Skerjanc IS
The EMBO journal30(12)2477-89(2011 May)

Single-molecule force spectroscopy distinguishes target binding modes of calmodulin.
Junker JP, Rief M
Proceedings of the National Academy of Sciences of the United States of America106(34)14361-6(2009 Aug)

Variation in the myosin light chain kinase gene is associated with development of acute lung injury after major trauma.
Christie JD, Ma SF, Aplenc R, Li M, Lanken PN, Shah CV, Fuchs B, Albelda SM, Flores C, Garcia JG
Critical care medicine36(10)2794-800(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chinese family with dual LQT1 and HCM phenotypes associated with tetrad heterozygous mutations in KCNQ1, MYH7, MYLK2, and TMEM70 mutations.
Title: Dual LQT1 and HCM phenotypes associated with tetrad heterozygous mutations in KCNQ1, MYH7, MYLK2, and TMEM70 genes in a three-generation Chinese family.

Data show that that the prototypical CaM target sequence skMLCK, a fragment from skeletal muscle myosin light chain kinase, binds to CaM in a highly cooperative way, while only a lower degree of interdomain binding cooperativity emerges for CaMKK.
Title: Single-molecule force spectroscopy distinguishes target binding modes of calmodulin.

MYLK gene is a risk factor for the development of acute lung injuries.
Title: Variation in the myosin light chain kinase gene is associated with development of acute lung injury after major trauma.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutational analysis of the kinase domain of MYLK2 gene in common human cancers.

the P2X1-ERK2-Myosin Light Chain Kinase axis contributes to collagen-induced platelet activation by enhancing platelet degranulation
Title: P2X1-mediated ERK2 activation amplifies the collagen-induced platelet secretion by enhancing myosin light chain kinase activation.

The following MYLK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19161	NM_033118.4 Latest version!	Homo sapiens myosin light chain kinase 2 (MYLK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu19161	NM_033118.3	Homo sapiens myosin light chain kinase 2 (MYLK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYLK2 ( NM_033118.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_149109.1 Homo sapiens myosin light chain kinase 2 (MYLK2), mRNA.

Price & Availability↑

CloneID	OHu19161
Clone ID Related Accession (Same CDS sequence)	NM_033118.4 , NM_033118.3
Accession Version	NM_033118.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	myosin light chain kinase 2, skeletal/cardiac muscle
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160175.5 and BC092413.1. On Aug 31, 2019 this sequence version replaced NM_033118.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092413.1, AJ272502.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACAG AAAATGGAGC AGTTGAGCTG GGAATTCAGA ACCCATCAAC AGACAAGGCA 
CCTAAAGGTC CCACAGGTGA AAGACCCCTG GCTGCAGGGA AAGACCCTGG CCCCCCAGAC 
CCAAAGAAAG CTCCGGATCC ACCCACCCTG AAGAAAGATG CCAAAGCCCC TGCCTCAGAG 
AAAGGGGATG GTACCCTGGC CCAACCCTCA ACTAGCAGCC AAGGCCCCAA AGGAGAGGGT 
GACAGGGGCG GGGGGCCCGC GGAGGGCAGT GCTGGGCCCC CGGCAGCCCT GCCCCAGCAG 
ACTGCGACAC CTGAGACCAG CGTCAAGAAG CCCAAGGCTG AGCAGGGAGC CTCAGGCAGC 
CAGGATCCTG GAAAGCCCAG GGTGGGCAAG AAGGCAGCAG AGGGCCAAGC AGCAGCCAGG 
AGGGGCTCAC CTGCCTTTCT GCATAGCCCC AGCTGTCCTG CCATCATCTC CAGTTCTGAG 
AAGCTGCTGG CCAAGAAGCC CCCAAGCGAG GCATCAGAGC TCACCTTTGA AGGGGTGCCC 
ATGACCCACA GCCCCACGGA TCCCAGGCCA GCCAAGGCAG AAGAAGGAAA GAACATCCTG 
GCAGAGAGCC AGAAGGAAGT GGGAGAGAAA ACCCCAGGCC AGGCTGGCCA GGCTAAGATG 
CAAGGGGACA CCTCGAGGGG GATTGAGTTC CAGGCTGTTC CCTCAGAGAA ATCCGAGGTG 
GGGCAGGCCC TCTGTCTCAC AGCCAGGGAG GAGGACTGCT TCCAGATTTT GGATGATTGC 
CCGCCACCTC CGGCCCCCTT CCCTCACCGC ATGGTGGAGC TGAGGACCGG GAATGTCAGC 
AGTGAATTCA GTATGAACTC CAAGGAGGCG CTCGGAGGTG GCAAGTTTGG GGCAGTCTGT 
ACCTGCATGG AGAAAGCCAC AGGCCTCAAG CTGGCAGCCA AGGTCATCAA GAAACAGACT 
CCCAAAGACA AGGAAATGGT GTTGCTGGAG ATTGAGGTCA TGAACCAGCT GAACCACCGC 
AATCTGATCC AGCTGTATGC AGCCATCGAG ACTCCGCATG AGATCGTCCT GTTCATGGAG 
TACATCGAGG GCGGAGAGCT CTTCGAGAGG ATTGTGGATG AGGACTACCA TCTGACCGAG 
GTGGACACCA TGGTGTTTGT CAGGCAGATC TGTGACGGGA TCCTCTTCAT GCACAAGATG 
AGGGTTTTGC ACCTGGACCT CAAGCCAGAG AACATCCTGT GTGTCAACAC CACCGGGCAT 
TTGGTGAAGA TCATTGACTT TGGCCTGGCA CGGAGGTATA ACCCCAACGA GAAGCTGAAG 
GTGAACTTTG GGACCCCAGA GTTCCTGTCA CCTGAGGTGG TGAATTATGA CCAAATCTCC 
GATAAGACAG ACATGTGGAG TATGGGGGTG ATCACCTACA TGCTGCTGAG CGGCCTCTCC 
CCCTTCCTGG GAGATGATGA CACAGAGACC CTAAACAACG TTCTATCTGG CAACTGGTAC 
TTTGATGAAG AGACCTTTGA GGCCGTATCA GACGAGGCCA AAGACTTTGT CTCCAACCTC 
ATCGTCAAGG ACCAGAGGGC CCGGATGAAC GCTGCCCAGT GTCTCGCCCA TCCCTGGCTC 
AACAACCTGG CGGAGAAAGC CAAACGCTGT AACCGACGCC TTAAGTCCCA GATCTTGCTT 
AAGAAATACC TCATGAAGAG GCGCTGGAAG AAAAACTTCA TTGCTGTCAG CGCTGCCAAC 
CGCTTCAAGA AGATCAGCAG CTCGGGGGCA CTGATGGCTC TGGGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149109.1
CDS	122..1912
Translation	MATENGAVELGIQNPSTDKAPKGPTGERPLAAGKDPGPPDPKKAPDPPTLKKDAKAPASEKGDGTLAQPSTSSQGPKGEGDRGGGPAEGSAGPPAALPQQTATPETSVKKPKAEQGASGSQDPGKPRVGKKAAEGQAAARRGSPAFLHSPSCPAIISSSEKLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDCPPPPAPFPHRMVELRTGNVSSEFSMNSKEALGGGKFGAVCTCMEKATGLKLAAKVIKKQTPKDKEMVLLEIEVMNQLNHRNLIQLYAAIETPHEIVLFMEYIEGGELFERIVDEDYHLTEVDTMVFVRQICDGILFMHKMRVLHLDLKPENILCVNTTGHLVKIIDFGLARRYNPNEKLKVNFGTPEFLSPEVVNYDQISDKTDMWSMGVITYMLLSGLSPFLGDDDTETLNNVLSGNWYFDEETFEAVSDEAKDFVSNLIVKDQRARMNAAQCLAHPWLNNLAEKAKRCNRRLKSQILLKKYLMKRRWKKNFIAVSAANRFKKISSSGALMALGV

Target ORF information:

RefSeq Version	NM_033118.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain kinase 2 (MYLK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033118.4

ORF Insert Sequence:

ATGGCGACAG AAAATGGAGC AGTTGAGCTG GGAATTCAGA ACCCATCAAC AGACAAGGCA 
CCTAAAGGTC CCACAGGTGA AAGACCCCTG GCTGCAGGGA AAGACCCTGG CCCCCCAGAC 
CCAAAGAAAG CTCCGGATCC ACCCACCCTG AAGAAAGATG CCAAAGCCCC TGCCTCAGAG 
AAAGGGGATG GTACCCTGGC CCAACCCTCA ACTAGCAGCC AAGGCCCCAA AGGAGAGGGT 
GACAGGGGCG GGGGGCCCGC GGAGGGCAGT GCTGGGCCCC CGGCAGCCCT GCCCCAGCAG 
ACTGCGACAC CTGAGACCAG CGTCAAGAAG CCCAAGGCTG AGCAGGGAGC CTCAGGCAGC 
CAGGATCCTG GAAAGCCCAG GGTGGGCAAG AAGGCAGCAG AGGGCCAAGC AGCAGCCAGG 
AGGGGCTCAC CTGCCTTTCT GCATAGCCCC AGCTGTCCTG CCATCATCTC CAGTTCTGAG 
AAGCTGCTGG CCAAGAAGCC CCCAAGCGAG GCATCAGAGC TCACCTTTGA AGGGGTGCCC 
ATGACCCACA GCCCCACGGA TCCCAGGCCA GCCAAGGCAG AAGAAGGAAA GAACATCCTG 
GCAGAGAGCC AGAAGGAAGT GGGAGAGAAA ACCCCAGGCC AGGCTGGCCA GGCTAAGATG 
CAAGGGGACA CCTCGAGGGG GATTGAGTTC CAGGCTGTTC CCTCAGAGAA ATCCGAGGTG 
GGGCAGGCCC TCTGTCTCAC AGCCAGGGAG GAGGACTGCT TCCAGATTTT GGATGATTGC 
CCGCCACCTC CGGCCCCCTT CCCTCACCGC ATGGTGGAGC TGAGGACCGG GAATGTCAGC 
AGTGAATTCA GTATGAACTC CAAGGAGGCG CTCGGAGGTG GCAAGTTTGG GGCAGTCTGT 
ACCTGCATGG AGAAAGCCAC AGGCCTCAAG CTGGCAGCCA AGGTCATCAA GAAACAGACT 
CCCAAAGACA AGGAAATGGT GTTGCTGGAG ATTGAGGTCA TGAACCAGCT GAACCACCGC 
AATCTGATCC AGCTGTATGC AGCCATCGAG ACTCCGCATG AGATCGTCCT GTTCATGGAG 
TACATCGAGG GCGGAGAGCT CTTCGAGAGG ATTGTGGATG AGGACTACCA TCTGACCGAG 
GTGGACACCA TGGTGTTTGT CAGGCAGATC TGTGACGGGA TCCTCTTCAT GCACAAGATG 
AGGGTTTTGC ACCTGGACCT CAAGCCAGAG AACATCCTGT GTGTCAACAC CACCGGGCAT 
TTGGTGAAGA TCATTGACTT TGGCCTGGCA CGGAGGTATA ACCCCAACGA GAAGCTGAAG 
GTGAACTTTG GGACCCCAGA GTTCCTGTCA CCTGAGGTGG TGAATTATGA CCAAATCTCC 
GATAAGACAG ACATGTGGAG TATGGGGGTG ATCACCTACA TGCTGCTGAG CGGCCTCTCC 
CCCTTCCTGG GAGATGATGA CACAGAGACC CTAAACAACG TTCTATCTGG CAACTGGTAC 
TTTGATGAAG AGACCTTTGA GGCCGTATCA GACGAGGCCA AAGACTTTGT CTCCAACCTC 
ATCGTCAAGG ACCAGAGGGC CCGGATGAAC GCTGCCCAGT GTCTCGCCCA TCCCTGGCTC 
AACAACCTGG CGGAGAAAGC CAAACGCTGT AACCGACGCC TTAAGTCCCA GATCTTGCTT 
AAGAAATACC TCATGAAGAG GCGCTGGAAG AAAAACTTCA TTGCTGTCAG CGCTGCCAAC 
CGCTTCAAGA AGATCAGCAG CTCGGGGGCA CTGATGGCTC TGGGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYLK2 ( NM_033118.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_149109.1 Homo sapiens myosin light chain kinase 2 (MYLK2), mRNA.

Price & Availability↑

CloneID	OHu19161
Clone ID Related Accession (Same CDS sequence)	NM_033118.4 , NM_033118.3
Accession Version	NM_033118.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	myosin light chain kinase 2, skeletal/cardiac muscle
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC092413.1 and F22840.1. This sequence is a reference standard in the RefSeqGene project. On Jan 27, 2008 this sequence version replaced NM_033118.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092413.1, AJ272502.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153347, SAMEA2158800 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACAG AAAATGGAGC AGTTGAGCTG GGAATTCAGA ACCCATCAAC AGACAAGGCA 
CCTAAAGGTC CCACAGGTGA AAGACCCCTG GCTGCAGGGA AAGACCCTGG CCCCCCAGAC 
CCAAAGAAAG CTCCGGATCC ACCCACCCTG AAGAAAGATG CCAAAGCCCC TGCCTCAGAG 
AAAGGGGATG GTACCCTGGC CCAACCCTCA ACTAGCAGCC AAGGCCCCAA AGGAGAGGGT 
GACAGGGGCG GGGGGCCCGC GGAGGGCAGT GCTGGGCCCC CGGCAGCCCT GCCCCAGCAG 
ACTGCGACAC CTGAGACCAG CGTCAAGAAG CCCAAGGCTG AGCAGGGAGC CTCAGGCAGC 
CAGGATCCTG GAAAGCCCAG GGTGGGCAAG AAGGCAGCAG AGGGCCAAGC AGCAGCCAGG 
AGGGGCTCAC CTGCCTTTCT GCATAGCCCC AGCTGTCCTG CCATCATCTC CAGTTCTGAG 
AAGCTGCTGG CCAAGAAGCC CCCAAGCGAG GCATCAGAGC TCACCTTTGA AGGGGTGCCC 
ATGACCCACA GCCCCACGGA TCCCAGGCCA GCCAAGGCAG AAGAAGGAAA GAACATCCTG 
GCAGAGAGCC AGAAGGAAGT GGGAGAGAAA ACCCCAGGCC AGGCTGGCCA GGCTAAGATG 
CAAGGGGACA CCTCGAGGGG GATTGAGTTC CAGGCTGTTC CCTCAGAGAA ATCCGAGGTG 
GGGCAGGCCC TCTGTCTCAC AGCCAGGGAG GAGGACTGCT TCCAGATTTT GGATGATTGC 
CCGCCACCTC CGGCCCCCTT CCCTCACCGC ATGGTGGAGC TGAGGACCGG GAATGTCAGC 
AGTGAATTCA GTATGAACTC CAAGGAGGCG CTCGGAGGTG GCAAGTTTGG GGCAGTCTGT 
ACCTGCATGG AGAAAGCCAC AGGCCTCAAG CTGGCAGCCA AGGTCATCAA GAAACAGACT 
CCCAAAGACA AGGAAATGGT GTTGCTGGAG ATTGAGGTCA TGAACCAGCT GAACCACCGC 
AATCTGATCC AGCTGTATGC AGCCATCGAG ACTCCGCATG AGATCGTCCT GTTCATGGAG 
TACATCGAGG GCGGAGAGCT CTTCGAGAGG ATTGTGGATG AGGACTACCA TCTGACCGAG 
GTGGACACCA TGGTGTTTGT CAGGCAGATC TGTGACGGGA TCCTCTTCAT GCACAAGATG 
AGGGTTTTGC ACCTGGACCT CAAGCCAGAG AACATCCTGT GTGTCAACAC CACCGGGCAT 
TTGGTGAAGA TCATTGACTT TGGCCTGGCA CGGAGGTATA ACCCCAACGA GAAGCTGAAG 
GTGAACTTTG GGACCCCAGA GTTCCTGTCA CCTGAGGTGG TGAATTATGA CCAAATCTCC 
GATAAGACAG ACATGTGGAG TATGGGGGTG ATCACCTACA TGCTGCTGAG CGGCCTCTCC 
CCCTTCCTGG GAGATGATGA CACAGAGACC CTAAACAACG TTCTATCTGG CAACTGGTAC 
TTTGATGAAG AGACCTTTGA GGCCGTATCA GACGAGGCCA AAGACTTTGT CTCCAACCTC 
ATCGTCAAGG ACCAGAGGGC CCGGATGAAC GCTGCCCAGT GTCTCGCCCA TCCCTGGCTC 
AACAACCTGG CGGAGAAAGC CAAACGCTGT AACCGACGCC TTAAGTCCCA GATCTTGCTT 
AAGAAATACC TCATGAAGAG GCGCTGGAAG AAAAACTTCA TTGCTGTCAG CGCTGCCAAC 
CGCTTCAAGA AGATCAGCAG CTCGGGGGCA CTGATGGCTC TGGGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149109.1
CDS	103..1893
Translation	MATENGAVELGIQNPSTDKAPKGPTGERPLAAGKDPGPPDPKKAPDPPTLKKDAKAPASEKGDGTLAQPSTSSQGPKGEGDRGGGPAEGSAGPPAALPQQTATPETSVKKPKAEQGASGSQDPGKPRVGKKAAEGQAAARRGSPAFLHSPSCPAIISSSEKLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDCPPPPAPFPHRMVELRTGNVSSEFSMNSKEALGGGKFGAVCTCMEKATGLKLAAKVIKKQTPKDKEMVLLEIEVMNQLNHRNLIQLYAAIETPHEIVLFMEYIEGGELFERIVDEDYHLTEVDTMVFVRQICDGILFMHKMRVLHLDLKPENILCVNTTGHLVKIIDFGLARRYNPNEKLKVNFGTPEFLSPEVVNYDQISDKTDMWSMGVITYMLLSGLSPFLGDDDTETLNNVLSGNWYFDEETFEAVSDEAKDFVSNLIVKDQRARMNAAQCLAHPWLNNLAEKAKRCNRRLKSQILLKKYLMKRRWKKNFIAVSAANRFKKISSSGALMALGV

Target ORF information:

RefSeq Version	NM_033118.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain kinase 2 (MYLK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033118.3

ORF Insert Sequence:

ATGGCGACAG AAAATGGAGC AGTTGAGCTG GGAATTCAGA ACCCATCAAC AGACAAGGCA 
CCTAAAGGTC CCACAGGTGA AAGACCCCTG GCTGCAGGGA AAGACCCTGG CCCCCCAGAC 
CCAAAGAAAG CTCCGGATCC ACCCACCCTG AAGAAAGATG CCAAAGCCCC TGCCTCAGAG 
AAAGGGGATG GTACCCTGGC CCAACCCTCA ACTAGCAGCC AAGGCCCCAA AGGAGAGGGT 
GACAGGGGCG GGGGGCCCGC GGAGGGCAGT GCTGGGCCCC CGGCAGCCCT GCCCCAGCAG 
ACTGCGACAC CTGAGACCAG CGTCAAGAAG CCCAAGGCTG AGCAGGGAGC CTCAGGCAGC 
CAGGATCCTG GAAAGCCCAG GGTGGGCAAG AAGGCAGCAG AGGGCCAAGC AGCAGCCAGG 
AGGGGCTCAC CTGCCTTTCT GCATAGCCCC AGCTGTCCTG CCATCATCTC CAGTTCTGAG 
AAGCTGCTGG CCAAGAAGCC CCCAAGCGAG GCATCAGAGC TCACCTTTGA AGGGGTGCCC 
ATGACCCACA GCCCCACGGA TCCCAGGCCA GCCAAGGCAG AAGAAGGAAA GAACATCCTG 
GCAGAGAGCC AGAAGGAAGT GGGAGAGAAA ACCCCAGGCC AGGCTGGCCA GGCTAAGATG 
CAAGGGGACA CCTCGAGGGG GATTGAGTTC CAGGCTGTTC CCTCAGAGAA ATCCGAGGTG 
GGGCAGGCCC TCTGTCTCAC AGCCAGGGAG GAGGACTGCT TCCAGATTTT GGATGATTGC 
CCGCCACCTC CGGCCCCCTT CCCTCACCGC ATGGTGGAGC TGAGGACCGG GAATGTCAGC 
AGTGAATTCA GTATGAACTC CAAGGAGGCG CTCGGAGGTG GCAAGTTTGG GGCAGTCTGT 
ACCTGCATGG AGAAAGCCAC AGGCCTCAAG CTGGCAGCCA AGGTCATCAA GAAACAGACT 
CCCAAAGACA AGGAAATGGT GTTGCTGGAG ATTGAGGTCA TGAACCAGCT GAACCACCGC 
AATCTGATCC AGCTGTATGC AGCCATCGAG ACTCCGCATG AGATCGTCCT GTTCATGGAG 
TACATCGAGG GCGGAGAGCT CTTCGAGAGG ATTGTGGATG AGGACTACCA TCTGACCGAG 
GTGGACACCA TGGTGTTTGT CAGGCAGATC TGTGACGGGA TCCTCTTCAT GCACAAGATG 
AGGGTTTTGC ACCTGGACCT CAAGCCAGAG AACATCCTGT GTGTCAACAC CACCGGGCAT 
TTGGTGAAGA TCATTGACTT TGGCCTGGCA CGGAGGTATA ACCCCAACGA GAAGCTGAAG 
GTGAACTTTG GGACCCCAGA GTTCCTGTCA CCTGAGGTGG TGAATTATGA CCAAATCTCC 
GATAAGACAG ACATGTGGAG TATGGGGGTG ATCACCTACA TGCTGCTGAG CGGCCTCTCC 
CCCTTCCTGG GAGATGATGA CACAGAGACC CTAAACAACG TTCTATCTGG CAACTGGTAC 
TTTGATGAAG AGACCTTTGA GGCCGTATCA GACGAGGCCA AAGACTTTGT CTCCAACCTC 
ATCGTCAAGG ACCAGAGGGC CCGGATGAAC GCTGCCCAGT GTCTCGCCCA TCCCTGGCTC 
AACAACCTGG CGGAGAAAGC CAAACGCTGT AACCGACGCC TTAAGTCCCA GATCTTGCTT 
AAGAAATACC TCATGAAGAG GCGCTGGAAG AAAAACTTCA TTGCTGTCAG CGCTGCCAAC 
CGCTTCAAGA AGATCAGCAG CTCGGGGGCA CTGATGGCTC TGGGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dual LQT1 and HCM phenotypes associated with tetrad heterozygous mutations in KCNQ1, MYH7, MYLK2, and TMEM70 genes in a three-generation Chinese family.
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology18(4)602-9(2016 Apr)
Wang L,Zuo L,Hu J,Shao H,Lei C,Qi W,Liu Y,Miao Y,Ma X,Huang CL,Wang B,Zhou X,Zhang Y,Liu L

Skeletal myosin light chain kinase regulates skeletal myogenesis by phosphorylation of MEF2C.
The EMBO journal30(12)2477-89(2011 May)
Al Madhoun AS,Mehta V,Li G,Figeys D,Wiper-Bergeron N,Skerjanc IS

Single-molecule force spectroscopy distinguishes target binding modes of calmodulin.
Proceedings of the National Academy of Sciences of the United States of America106(34)14361-6(2009 Aug)
Junker JP,Rief M

Variation in the myosin light chain kinase gene is associated with development of acute lung injury after major trauma.
Critical care medicine36(10)2794-800(2008 Oct)
Christie JD,Ma SF,Aplenc R,Li M,Lanken PN,Shah CV,Fuchs B,Albelda SM,Flores C,Garcia JG

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MYLK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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