COL27A1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017015239.1	XP_016870728.1	collagen alpha-1(XXVII) chain isoform X7
XM_011519142.2	XP_011517444.1	collagen alpha-1(XXVII) chain isoform X4
XM_006717310.3	XP_006717373.1	collagen alpha-1(XXVII) chain isoform X8
NM_032888.3	NP_116277.2	collagen alpha-1(XXVII) chain preproprotein
NM_032888.4	NP_116277.2	collagen alpha-1(XXVII) chain preproprotein
XM_011519138.2	XP_011517440.1	collagen alpha-1(XXVII) chain isoform X1
XM_011519140.1	XP_011517442.1	collagen alpha-1(XXVII) chain isoform X2
XM_011519142.3	XP_011517444.1	collagen alpha-1(XXVII) chain isoform X4
XM_011519143.2	XP_011517445.1	collagen alpha-1(XXVII) chain isoform X5
XM_011519144.2	XP_011517446.1	collagen alpha-1(XXVII) chain isoform X6
XM_011519145.3	XP_011517447.1	collagen alpha-1(XXVII) chain isoform X9
XM_011519145.2	XP_011517447.1	collagen alpha-1(XXVII) chain isoform X9
XM_006717308.2	XP_006717371.1	collagen alpha-1(XXVII) chain isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13132	NM_032888.4 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu43564	XM_006717308.2 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu43566	XM_006717310.3 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu73932	XM_011519138.2 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu73934	XM_011519140.1 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu73936	XM_011519142.3 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu73937	XM_011519143.2 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu73938	XM_011519144.2 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu73939	XM_011519145.3 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu98967	XM_017015239.1 Latest version!	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu13132	NM_032888.3	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu73936	XM_011519142.2	Homo sapiens collagen type XXVII alpha 1 (COL27A1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu73939	XM_011519145.2	Homo sapiens collagen type XXVII alpha 1 (COL27A1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
Hide Old Accession Versions >>

COL27A1 ( NM_032888.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116277.2 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), mRNA.

Price & Availability↑

CloneID	OHu13132
Clone ID Related Accession (Same CDS sequence)	NM_032888.3 , NM_032888.4
Accession Version	NM_032888.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU076480.1, BC035728.1, AY149237.1, AL356796.16 and AI808820.1. This sequence is a reference standard in the RefSeqGene project. On Sep 17, 2014 this sequence version replaced NM_032888.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY149237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG TGAGATGGGC GTCCCTGGAG ACCCTGGACC CCCTGGCACT 
CCAGGCCCTA AAGGGTCCCG GGGCAGCCTG GGACCAACGG GTGCTCCGGG ACGCATGGGG 
GCCCAAGGAG AACCGGGACT GGCTGGTTAT GATGGACACA AAGGCATTGT GGGACCCCTT 
GGACCTCCTG GACCAAAAGG CGAAAAGGGG GAGCAGGGCG AGGACGGCAA GGCTGAGGGG 
CCCCCTGGGC CACCTGGAGA TCGGGGCCCT GTGGGTGATC GAGGAGACCG CGGGGAACCG 
GGAGACCCTG GGTACCCTGG ACAGGAGGGT GTGCAAGGCC TCCGTGGAAA GCCAGGCCAG 
CAGGGCCAAC CCGGGCATCC GGGACCCCGG GGGTGGCCGG GACCCAAAGG ATCGAAAGGC 
GCAGAGGGAC CAAAGGGAAA GCAAGGCAAG GCAGGGGCCC CAGGCCGGAG GGGGGTCCAG 
GGCCTGCAGG GGCTGCCAGG GCCCCGGGGC GTGGTGGGGA GACAGGGCCT CGAGGGCATC 
GCTGGACCAG ATGGGCTTCC TGGCAGGGAC GGGCAAGCAG GACAGCAGGG GGAGCAGGGA 
GACGATGGGG ACCCTGGCCC CATGGGCCCT GCTGGGAAGA GAGGAAATCC AGGTGTGGCC 
GGCTTACCTG GAGCACAGGG ACCCCCAGGA TTCAAGGGTG AGAGTGGGTT ACCCGGACAG 
CTGGGTCCCC CTGGCAAGCG AGGAACAGAG GGCAGAACGG GGCTCCCTGG AAACCAGGGG 
GAGCCTGGGT CCAAAGGCCA GCCGGGCGAC TCTGGCGAGA TGGGCTTCCC AGGAATGGCA 
GGTCTCTTCG GACCCAAGGG CCCGCCTGGA GACATTGGCT TCAAAGGCAT CCAGGGCCCT 
CGGGGGCCAC CTGGCTTGAT GGGAAAGGAA GGCATCGTCG GGCCCCTCGG AATCCTGGGA 
CCTTCGGGAC TCCCGGGTCC GAAGGGTGAC AAAGGCAGCC GTGGGGACTG GGGATTGCAA 
GGTCCGAGGG GTCCTCCCGG CCCCAGAGGG CGGCCCGGCC CCCCGGGTCC TCCAGGGGGT 
CCTATCCAAT TGCAACAAGA TGATCTTGGG GCAGCTTTCC AGACGTGGAT GGACACCAGT 
GGAGCACTCA GGCCAGAGAG TTACAGCTAT CCAGACCGGC TGGTGCTGGA CCAGGGAGGA 
GAGATCTTTA AAACCTTACA CTACCTCAGC AACCTCATCC AGAGCATTAA GACGCCCCTG 
GGCACCAAAG AGAACCCCGC CCGGGTCTGC AGGGACCTCA TGGACTGTGA GCAGAAGATG 
GTGGATGGTA CCTACTGGGT GGATCCAAAC CTTGGCTGCT CCTCTGACAC CATCGAGGTC 
TCCTGCAACT TCACTCATGG TGGACAGACG TGTCTCAAGC CCATCACGGC CTCCAAGGTC 
GAGTTTGCCA TCAGCCGGGT CCAGATGAAT TTCCTGCACC TGCTAAGCTC CGAGGTGACC 
CAGCACATCA CCATCCACTG CCTTAACATG ACCGTGTGGC AGGAGGGCAC TGGGCAGACC 
CCAGCCAAGC AGGCCGTACG CTTCCGGGCC TGGAATGGAC AGATTTTTGA AGCTGGGGGT 
CAGTTCCGGC CCGAGGTGTC CATGGATGGC TGCAAGGTCC AAGATGGCCG CTGGCATCAG 
ACACTCTTCA CCTTCCGGAC CCAAGACCCC CAACAGCTGC CCATCATCAG TGTGGACAAC 
CTCCCTCCTG CCTCATCAGG GAAGCAGTAC CGCCTGGAAG TTGGACCTGC GTGCTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116277.2
CDS	407..5989
Translation	MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	NM_032888.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032888.3

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG TGAGATGGGC GTCCCTGGAG ACCCTGGACC CCCTGGCACT 
CCAGGCCCTA AAGGGTCCCG GGGCAGCCTG GGACCAACGG GTGCTCCGGG ACGCATGGGG 
GCCCAAGGAG AACCGGGACT GGCTGGTTAT GATGGACACA AAGGCATTGT GGGACCCCTT 
GGACCTCCTG GACCAAAAGG CGAAAAGGGG GAGCAGGGCG AGGACGGCAA GGCTGAGGGG 
CCCCCTGGGC CACCTGGAGA TCGGGGCCCT GTGGGTGATC GAGGAGACCG CGGGGAACCG 
GGAGACCCTG GGTACCCTGG ACAGGAGGGT GTGCAAGGCC TCCGTGGAAA GCCAGGCCAG 
CAGGGCCAAC CCGGGCATCC GGGACCCCGG GGGTGGCCGG GACCCAAAGG ATCGAAAGGC 
GCAGAGGGAC CAAAGGGAAA GCAAGGCAAG GCAGGGGCCC CAGGCCGGAG GGGGGTCCAG 
GGCCTGCAGG GGCTGCCAGG GCCCCGGGGC GTGGTGGGGA GACAGGGCCT CGAGGGCATC 
GCTGGACCAG ATGGGCTTCC TGGCAGGGAC GGGCAAGCAG GACAGCAGGG GGAGCAGGGA 
GACGATGGGG ACCCTGGCCC CATGGGCCCT GCTGGGAAGA GAGGAAATCC AGGTGTGGCC 
GGCTTACCTG GAGCACAGGG ACCCCCAGGA TTCAAGGGTG AGAGTGGGTT ACCCGGACAG 
CTGGGTCCCC CTGGCAAGCG AGGAACAGAG GGCAGAACGG GGCTCCCTGG AAACCAGGGG 
GAGCCTGGGT CCAAAGGCCA GCCGGGCGAC TCTGGCGAGA TGGGCTTCCC AGGAATGGCA 
GGTCTCTTCG GACCCAAGGG CCCGCCTGGA GACATTGGCT TCAAAGGCAT CCAGGGCCCT 
CGGGGGCCAC CTGGCTTGAT GGGAAAGGAA GGCATCGTCG GGCCCCTCGG AATCCTGGGA 
CCTTCGGGAC TCCCGGGTCC GAAGGGTGAC AAAGGCAGCC GTGGGGACTG GGGATTGCAA 
GGTCCGAGGG GTCCTCCCGG CCCCAGAGGG CGGCCCGGCC CCCCGGGTCC TCCAGGGGGT 
CCTATCCAAT TGCAACAAGA TGATCTTGGG GCAGCTTTCC AGACGTGGAT GGACACCAGT 
GGAGCACTCA GGCCAGAGAG TTACAGCTAT CCAGACCGGC TGGTGCTGGA CCAGGGAGGA 
GAGATCTTTA AAACCTTACA CTACCTCAGC AACCTCATCC AGAGCATTAA GACGCCCCTG 
GGCACCAAAG AGAACCCCGC CCGGGTCTGC AGGGACCTCA TGGACTGTGA GCAGAAGATG 
GTGGATGGTA CCTACTGGGT GGATCCAAAC CTTGGCTGCT CCTCTGACAC CATCGAGGTC 
TCCTGCAACT TCACTCATGG TGGACAGACG TGTCTCAAGC CCATCACGGC CTCCAAGGTC 
GAGTTTGCCA TCAGCCGGGT CCAGATGAAT TTCCTGCACC TGCTAAGCTC CGAGGTGACC 
CAGCACATCA CCATCCACTG CCTTAACATG ACCGTGTGGC AGGAGGGCAC TGGGCAGACC 
CCAGCCAAGC AGGCCGTACG CTTCCGGGCC TGGAATGGAC AGATTTTTGA AGCTGGGGGT 
CAGTTCCGGC CCGAGGTGTC CATGGATGGC TGCAAGGTCC AAGATGGCCG CTGGCATCAG 
ACACTCTTCA CCTTCCGGAC CCAAGACCCC CAACAGCTGC CCATCATCAG TGTGGACAAC 
CTCCCTCCTG CCTCATCAGG GAAGCAGTAC CGCCTGGAAG TTGGACCTGC GTGCTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( NM_032888.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_116277.2 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), mRNA.

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CloneID	OHu13132
Clone ID Related Accession (Same CDS sequence)	NM_032888.3 , NM_032888.4
Accession Version	NM_032888.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL445543.20, BU076480.1, BC035728.1, AY149237.1, AL356796.16 and AI808820.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_032888.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY149237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000356083.8/ ENSP00000348385.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG TGAGATGGGC GTCCCTGGAG ACCCTGGACC CCCTGGCACT 
CCAGGCCCTA AAGGGTCCCG GGGCAGCCTG GGACCAACGG GTGCTCCGGG ACGCATGGGG 
GCCCAAGGAG AACCGGGACT GGCTGGTTAT GATGGACACA AAGGCATTGT GGGACCCCTT 
GGACCTCCTG GACCAAAAGG CGAAAAGGGG GAGCAGGGCG AGGACGGCAA GGCTGAGGGG 
CCCCCTGGGC CACCTGGAGA TCGGGGCCCT GTGGGTGATC GAGGAGACCG CGGGGAACCG 
GGAGACCCTG GGTACCCTGG ACAGGAGGGT GTGCAAGGCC TCCGTGGAAA GCCAGGCCAG 
CAGGGCCAAC CCGGGCATCC GGGACCCCGG GGGTGGCCGG GACCCAAAGG ATCGAAAGGC 
GCAGAGGGAC CAAAGGGAAA GCAAGGCAAG GCAGGGGCCC CAGGCCGGAG GGGGGTCCAG 
GGCCTGCAGG GGCTGCCAGG GCCCCGGGGC GTGGTGGGGA GACAGGGCCT CGAGGGCATC 
GCTGGACCAG ATGGGCTTCC TGGCAGGGAC GGGCAAGCAG GACAGCAGGG GGAGCAGGGA 
GACGATGGGG ACCCTGGCCC CATGGGCCCT GCTGGGAAGA GAGGAAATCC AGGTGTGGCC 
GGCTTACCTG GAGCACAGGG ACCCCCAGGA TTCAAGGGTG AGAGTGGGTT ACCCGGACAG 
CTGGGTCCCC CTGGCAAGCG AGGAACAGAG GGCAGAACGG GGCTCCCTGG AAACCAGGGG 
GAGCCTGGGT CCAAAGGCCA GCCGGGCGAC TCTGGCGAGA TGGGCTTCCC AGGAATGGCA 
GGTCTCTTCG GACCCAAGGG CCCGCCTGGA GACATTGGCT TCAAAGGCAT CCAGGGCCCT 
CGGGGGCCAC CTGGCTTGAT GGGAAAGGAA GGCATCGTCG GGCCCCTCGG AATCCTGGGA 
CCTTCGGGAC TCCCGGGTCC GAAGGGTGAC AAAGGCAGCC GTGGGGACTG GGGATTGCAA 
GGTCCGAGGG GTCCTCCCGG CCCCAGAGGG CGGCCCGGCC CCCCGGGTCC TCCAGGGGGT 
CCTATCCAAT TGCAACAAGA TGATCTTGGG GCAGCTTTCC AGACGTGGAT GGACACCAGT 
GGAGCACTCA GGCCAGAGAG TTACAGCTAT CCAGACCGGC TGGTGCTGGA CCAGGGAGGA 
GAGATCTTTA AAACCTTACA CTACCTCAGC AACCTCATCC AGAGCATTAA GACGCCCCTG 
GGCACCAAAG AGAACCCCGC CCGGGTCTGC AGGGACCTCA TGGACTGTGA GCAGAAGATG 
GTGGATGGTA CCTACTGGGT GGATCCAAAC CTTGGCTGCT CCTCTGACAC CATCGAGGTC 
TCCTGCAACT TCACTCATGG TGGACAGACG TGTCTCAAGC CCATCACGGC CTCCAAGGTC 
GAGTTTGCCA TCAGCCGGGT CCAGATGAAT TTCCTGCACC TGCTAAGCTC CGAGGTGACC 
CAGCACATCA CCATCCACTG CCTTAACATG ACCGTGTGGC AGGAGGGCAC TGGGCAGACC 
CCAGCCAAGC AGGCCGTACG CTTCCGGGCC TGGAATGGAC AGATTTTTGA AGCTGGGGGT 
CAGTTCCGGC CCGAGGTGTC CATGGATGGC TGCAAGGTCC AAGATGGCCG CTGGCATCAG 
ACACTCTTCA CCTTCCGGAC CCAAGACCCC CAACAGCTGC CCATCATCAG TGTGGACAAC 
CTCCCTCCTG CCTCATCAGG GAAGCAGTAC CGCCTGGAAG TTGGACCTGC GTGCTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116277.2
CDS	415..5997
Translation	MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	NM_032888.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032888.4

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG TGAGATGGGC GTCCCTGGAG ACCCTGGACC CCCTGGCACT 
CCAGGCCCTA AAGGGTCCCG GGGCAGCCTG GGACCAACGG GTGCTCCGGG ACGCATGGGG 
GCCCAAGGAG AACCGGGACT GGCTGGTTAT GATGGACACA AAGGCATTGT GGGACCCCTT 
GGACCTCCTG GACCAAAAGG CGAAAAGGGG GAGCAGGGCG AGGACGGCAA GGCTGAGGGG 
CCCCCTGGGC CACCTGGAGA TCGGGGCCCT GTGGGTGATC GAGGAGACCG CGGGGAACCG 
GGAGACCCTG GGTACCCTGG ACAGGAGGGT GTGCAAGGCC TCCGTGGAAA GCCAGGCCAG 
CAGGGCCAAC CCGGGCATCC GGGACCCCGG GGGTGGCCGG GACCCAAAGG ATCGAAAGGC 
GCAGAGGGAC CAAAGGGAAA GCAAGGCAAG GCAGGGGCCC CAGGCCGGAG GGGGGTCCAG 
GGCCTGCAGG GGCTGCCAGG GCCCCGGGGC GTGGTGGGGA GACAGGGCCT CGAGGGCATC 
GCTGGACCAG ATGGGCTTCC TGGCAGGGAC GGGCAAGCAG GACAGCAGGG GGAGCAGGGA 
GACGATGGGG ACCCTGGCCC CATGGGCCCT GCTGGGAAGA GAGGAAATCC AGGTGTGGCC 
GGCTTACCTG GAGCACAGGG ACCCCCAGGA TTCAAGGGTG AGAGTGGGTT ACCCGGACAG 
CTGGGTCCCC CTGGCAAGCG AGGAACAGAG GGCAGAACGG GGCTCCCTGG AAACCAGGGG 
GAGCCTGGGT CCAAAGGCCA GCCGGGCGAC TCTGGCGAGA TGGGCTTCCC AGGAATGGCA 
GGTCTCTTCG GACCCAAGGG CCCGCCTGGA GACATTGGCT TCAAAGGCAT CCAGGGCCCT 
CGGGGGCCAC CTGGCTTGAT GGGAAAGGAA GGCATCGTCG GGCCCCTCGG AATCCTGGGA 
CCTTCGGGAC TCCCGGGTCC GAAGGGTGAC AAAGGCAGCC GTGGGGACTG GGGATTGCAA 
GGTCCGAGGG GTCCTCCCGG CCCCAGAGGG CGGCCCGGCC CCCCGGGTCC TCCAGGGGGT 
CCTATCCAAT TGCAACAAGA TGATCTTGGG GCAGCTTTCC AGACGTGGAT GGACACCAGT 
GGAGCACTCA GGCCAGAGAG TTACAGCTAT CCAGACCGGC TGGTGCTGGA CCAGGGAGGA 
GAGATCTTTA AAACCTTACA CTACCTCAGC AACCTCATCC AGAGCATTAA GACGCCCCTG 
GGCACCAAAG AGAACCCCGC CCGGGTCTGC AGGGACCTCA TGGACTGTGA GCAGAAGATG 
GTGGATGGTA CCTACTGGGT GGATCCAAAC CTTGGCTGCT CCTCTGACAC CATCGAGGTC 
TCCTGCAACT TCACTCATGG TGGACAGACG TGTCTCAAGC CCATCACGGC CTCCAAGGTC 
GAGTTTGCCA TCAGCCGGGT CCAGATGAAT TTCCTGCACC TGCTAAGCTC CGAGGTGACC 
CAGCACATCA CCATCCACTG CCTTAACATG ACCGTGTGGC AGGAGGGCAC TGGGCAGACC 
CCAGCCAAGC AGGCCGTACG CTTCCGGGCC TGGAATGGAC AGATTTTTGA AGCTGGGGGT 
CAGTTCCGGC CCGAGGTGTC CATGGATGGC TGCAAGGTCC AAGATGGCCG CTGGCATCAG 
ACACTCTTCA CCTTCCGGAC CCAAGACCCC CAACAGCTGC CCATCATCAG TGTGGACAAC 
CTCCCTCCTG CCTCATCAGG GAAGCAGTAC CGCCTGGAAG TTGGACCTGC GTGCTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_006717308.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717371.1 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.

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CloneID	OHu43564
Clone ID Related Accession (Same CDS sequence)	XM_006717308.2
Accession Version	XM_006717308.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006717308.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGTG ACATGGGCTT GCCTGGGCTC 
TCCGGGAATC CAGGACCTCC GGGACGAAAG GGACACAAGG GCTATCCTGG ACCGGCAGGG 
CACCCCGGAG AACAGGGGCA GCCAGGACCT GAGGGCAGCC CAGGGGCCAA AGGTTACCCT 
GGCAGGCAGG GGTTACCTGG ACCGGTAGGA GATCCCGGCC CCAAAGGCAG CAGGGGCTAC 
ATTGGGCTCC CAGGGCTCTT CGGCCTGCCA GGGTCTGATG GAGAACGAGG CCTGCCTGGC 
GTTCCTGGCA AGAGGGGCAA GATGGGTATG CCGGGGTTTC CTGGAGTCTT TGGGGAAAGA 
GGCCCTCCTG GACTGGATGG AAATCCTGGA GAACTGGGCC TGCCAGGCCC CCCTGGAGTC 
CCCGGCCTCA TTGGTGACTT AGGAGTGTTG GGTCCGATTG GCTACCCGGG ACCCAAGGGC 
ATGAAGGGAC TGATGGGCAG CGTGGGGGAG CCCGGACTGA AAGGTGATAA GGGTGAACAA 
GGGGTTCCAG GTGTGTCAGG AGATCCCGGA TTCCAAGGAG ACAAGGGGAG CCAGGGGTTG 
CCAGGGTTCC CCGGTGCACG GGGGAAGCCA GGGCCTCTGG GCAAAGTCGG AGACAAAGGA 
TCCATTGGGT TTCCCGGGCC CCCTGGACCC GAGGGATTCC CAGGAGACAT CGGCCCCCCT 
GGCGACAATG GCCCAGAAGG CATGAAGGGT AAGCCTGGAG CCCGAGGCCT GCCGGGACCC 
CGTGGGCAGC TGGGGCCCGA GGGAGATGAG GGACCCATGG GGCCGCCAGG GGCCCCTGGC 
TTGGAGGGTC AGCCTGGCAG GAAGGGGTTT CCTGGGAGGC CCGGCCTGGA TGGCGTGAAG 
GGGGAACCAG GGGATCCTGG TCGGCCGGGG CCTGTGGGAG AGCAGGGATT TATGGGATTC 
ATTGGTCTGG TCGGGGAGCC AGGAATCGTG GGAGAAAAGG GTGATCGTGG CATGATGGGA 
CCCCCAGGCG TGCCTGGACC CAAGGGGTCG ATGGGTCATC CTGGAATGCC AGGTGGTATG 
GGGACCCCTG GAGAGCCTGG ACCCCAGGGT CCTCCAGGAT CTCGAGGCCC ACCAGGCATG 
AGGGGAGCAA AGGGACGTCG GGGCCCCCGA GGACCGGACG GACCAGCTGG GGAGCAAGGG 
TCCAGGGGCC TGAAGGGCCC TCCAGGACCC CAGGGCAGAC CGGGCCGGCC TGGACAGCAG 
GGTGTGGCTG GTGAGCGAGG CCACTTGGGC TCGAGAGGCT TTCCTGGCAT CCCGGGTCCC 
TCAGGCCCCC CAGGCACCAA GGGCCTCCCA GGAGAACCGG GCCCTCAGGG ACCCCAGGGG 
CCAATTGGGC CTCCAGGAGA GATGGGACCC AAGGGGCCGC CTGGTGCAGT GGGAGAACCG 
GGCCTTCCTG GGGAAGCCGG GATGAAGGGT GACCTTGGAC CCCTGGGCAC TCCTGGGGAG 
CAGGGCCTCA TTGGGCAACG GGGAGAGCCA GGCCTTGAGG GTGACAGTGG CCCCATGGGA 
CCTGATGGGC TGAAGGGGGA CAGGGGAGAC CCAGGGCCTG ATGGAGAACA TGGCGAGAAA 
GGCCAGGAAG GGCTGATGGG TGAGGACGGG CCCCCCGGCC CCCCTGGCGT CACTGGTGTC 
CGGGGTCCTG AAGGAAAATC AGGGAAGCAA GGCGAGAAGG GCCGCACTGG AGCCAAGGGT 
GCCAAGGGCT ATCAAGGACA GCTGGGTGAG ATGGGCGTCC CTGGAGACCC TGGACCCCCT 
GGCACTCCAG GCCCTAAAGG GTCCCGGGGC AGCCTGGGAC CAACGGGTGC TCCGGGACGC 
ATGGGGGCCC AAGGAGAACC GGGACTGGCT GGTTATGATG GACACAAAGG CATTGTGGGA 
CCCCTTGGAC CTCCTGGACC AAAAGGCGAA AAGGGGGAGC AGGGCGAGGA CGGCAAGGCT 
GAGGGGCCCC CTGGGCCACC TGGAGATCGG GGCCCTGTGG GTGATCGAGG AGACCGCGGG 
GAACCGGGAG ACCCTGGGTA CCCTGGACAG GAGGGTGTGC AAGGCCTCCG TGGAAAGCCA 
GGCCAGCAGG GCCAACCCGG GCATCCGGGA CCCCGGGGGT GGCCGGGACC CAAAGGATCG 
AAAGGCGCAG AGGGACCAAA GGGAAAGCAA GGCAAGGCAG GGGCCCCAGG CCGGAGGGGG 
GTCCAGGGCC TGCAGGGGCT GCCAGGGCCC CGGGGCGTGG TGGGGAGACA GGGCCTCGAG 
GGCATCGCTG GACCAGATGG GCTTCCTGGC AGGGACGGGC AAGCAGGACA GCAGGGGGAG 
CAGGGAGACG ATGGGGACCC TGGCCCCATG GGCCCTGCTG GGAAGAGAGG AAATCCAGGT 
GTGGCCGGCT TACCTGGAGC ACAGGGACCC CCAGGATTCA AGGGTGAGAG TGGGTTACCC 
GGACAGCTGG GTCCCCCTGG CAAGCGAGGA ACAGAGGGCA GAACGGGGCT CCCTGGAAAC 
CAGGGGGAGC CTGGGTCCAA AGGCCAGCCG GGCGACTCTG GCGAGATGGG CTTCCCAGGA 
ATGGCAGGTC TCTTCGGACC CAAGGGCCCG CCTGGAGACA TTGGCTTCAA AGGCATCCAG 
GGCCCTCGGG GGCCACCTGG CTTGATGGGA AAGGAAGGCA TCGTCGGGCC CCTCGGAATC 
CTGGGACCTT CGGGACTCCC GGGTCCGAAG GGTGACAAAG GCAGCCGTGG GGACTGGGGA 
TTGCAAGGTC CGAGGGGTCC TCCCGGCCCC AGAGGGCGGC CCGGCCCCCC GGGTCCTCCA 
GGGGGTCCTA TCCAATTGCA ACAAGATGAT CTTGGGGCAG CTTTCCAGAC GTGGATGGAC 
ACCAGTGGAG CACTCAGGCC AGAGAGTTAC AGCTATCCAG ACCGGCTGGT GCTGGACCAG 
GGAGGAGAGA TCTTTAAAAC CTTACACTAC CTCAGCAACC TCATCCAGAG CATTAAGACG 
CCCCTGGGCA CCAAAGAGAA CCCCGCCCGG GTCTGCAGGG ACCTCATGGA CTGTGAGCAG 
AAGATGGTGG ATGGTACCTA CTGGGTGGAT CCAAACCTTG GCTGCTCCTC TGACACCATC 
GAGGTCTCCT GCAACTTCAC TCATGGTGGA CAGACGTGTC TCAAGCCCAT CACGGCCTCC 
AAGGTCGAGT TTGCCATCAG CCGGGTCCAG ATGAATTTCC TGCACCTGCT AAGCTCCGAG 
GTGACCCAGC ACATCACCAT CCACTGCCTT AACATGACCG TGTGGCAGGA GGGCACTGGG 
CAGACCCCAG CCAAGCAGGC CGTACGCTTC CGGGCCTGGA ATGGACAGAT TTTTGAAGCT 
GGGGGTCAGT TCCGGCCCGA GGTGTCCATG GATGGCTGCA AGGTCCAAGA TGGCCGCTGG 
CATCAGACAC TCTTCACCTT CCGGACCCAA GACCCCCAAC AGCTGCCCAT CATCAGTGTG 
GACAACCTCC CTCCTGCCTC ATCAGGGAAG CAGTACCGCC TGGAAGTTGG ACCTGCGTGC 
TTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717371.1
CDS	475..6003
Translation	MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_006717308.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717308.2

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGTG ACATGGGCTT GCCTGGGCTC 
TCCGGGAATC CAGGACCTCC GGGACGAAAG GGACACAAGG GCTATCCTGG ACCGGCAGGG 
CACCCCGGAG AACAGGGGCA GCCAGGACCT GAGGGCAGCC CAGGGGCCAA AGGTTACCCT 
GGCAGGCAGG GGTTACCTGG ACCGGTAGGA GATCCCGGCC CCAAAGGCAG CAGGGGCTAC 
ATTGGGCTCC CAGGGCTCTT CGGCCTGCCA GGGTCTGATG GAGAACGAGG CCTGCCTGGC 
GTTCCTGGCA AGAGGGGCAA GATGGGTATG CCGGGGTTTC CTGGAGTCTT TGGGGAAAGA 
GGCCCTCCTG GACTGGATGG AAATCCTGGA GAACTGGGCC TGCCAGGCCC CCCTGGAGTC 
CCCGGCCTCA TTGGTGACTT AGGAGTGTTG GGTCCGATTG GCTACCCGGG ACCCAAGGGC 
ATGAAGGGAC TGATGGGCAG CGTGGGGGAG CCCGGACTGA AAGGTGATAA GGGTGAACAA 
GGGGTTCCAG GTGTGTCAGG AGATCCCGGA TTCCAAGGAG ACAAGGGGAG CCAGGGGTTG 
CCAGGGTTCC CCGGTGCACG GGGGAAGCCA GGGCCTCTGG GCAAAGTCGG AGACAAAGGA 
TCCATTGGGT TTCCCGGGCC CCCTGGACCC GAGGGATTCC CAGGAGACAT CGGCCCCCCT 
GGCGACAATG GCCCAGAAGG CATGAAGGGT AAGCCTGGAG CCCGAGGCCT GCCGGGACCC 
CGTGGGCAGC TGGGGCCCGA GGGAGATGAG GGACCCATGG GGCCGCCAGG GGCCCCTGGC 
TTGGAGGGTC AGCCTGGCAG GAAGGGGTTT CCTGGGAGGC CCGGCCTGGA TGGCGTGAAG 
GGGGAACCAG GGGATCCTGG TCGGCCGGGG CCTGTGGGAG AGCAGGGATT TATGGGATTC 
ATTGGTCTGG TCGGGGAGCC AGGAATCGTG GGAGAAAAGG GTGATCGTGG CATGATGGGA 
CCCCCAGGCG TGCCTGGACC CAAGGGGTCG ATGGGTCATC CTGGAATGCC AGGTGGTATG 
GGGACCCCTG GAGAGCCTGG ACCCCAGGGT CCTCCAGGAT CTCGAGGCCC ACCAGGCATG 
AGGGGAGCAA AGGGACGTCG GGGCCCCCGA GGACCGGACG GACCAGCTGG GGAGCAAGGG 
TCCAGGGGCC TGAAGGGCCC TCCAGGACCC CAGGGCAGAC CGGGCCGGCC TGGACAGCAG 
GGTGTGGCTG GTGAGCGAGG CCACTTGGGC TCGAGAGGCT TTCCTGGCAT CCCGGGTCCC 
TCAGGCCCCC CAGGCACCAA GGGCCTCCCA GGAGAACCGG GCCCTCAGGG ACCCCAGGGG 
CCAATTGGGC CTCCAGGAGA GATGGGACCC AAGGGGCCGC CTGGTGCAGT GGGAGAACCG 
GGCCTTCCTG GGGAAGCCGG GATGAAGGGT GACCTTGGAC CCCTGGGCAC TCCTGGGGAG 
CAGGGCCTCA TTGGGCAACG GGGAGAGCCA GGCCTTGAGG GTGACAGTGG CCCCATGGGA 
CCTGATGGGC TGAAGGGGGA CAGGGGAGAC CCAGGGCCTG ATGGAGAACA TGGCGAGAAA 
GGCCAGGAAG GGCTGATGGG TGAGGACGGG CCCCCCGGCC CCCCTGGCGT CACTGGTGTC 
CGGGGTCCTG AAGGAAAATC AGGGAAGCAA GGCGAGAAGG GCCGCACTGG AGCCAAGGGT 
GCCAAGGGCT ATCAAGGACA GCTGGGTGAG ATGGGCGTCC CTGGAGACCC TGGACCCCCT 
GGCACTCCAG GCCCTAAAGG GTCCCGGGGC AGCCTGGGAC CAACGGGTGC TCCGGGACGC 
ATGGGGGCCC AAGGAGAACC GGGACTGGCT GGTTATGATG GACACAAAGG CATTGTGGGA 
CCCCTTGGAC CTCCTGGACC AAAAGGCGAA AAGGGGGAGC AGGGCGAGGA CGGCAAGGCT 
GAGGGGCCCC CTGGGCCACC TGGAGATCGG GGCCCTGTGG GTGATCGAGG AGACCGCGGG 
GAACCGGGAG ACCCTGGGTA CCCTGGACAG GAGGGTGTGC AAGGCCTCCG TGGAAAGCCA 
GGCCAGCAGG GCCAACCCGG GCATCCGGGA CCCCGGGGGT GGCCGGGACC CAAAGGATCG 
AAAGGCGCAG AGGGACCAAA GGGAAAGCAA GGCAAGGCAG GGGCCCCAGG CCGGAGGGGG 
GTCCAGGGCC TGCAGGGGCT GCCAGGGCCC CGGGGCGTGG TGGGGAGACA GGGCCTCGAG 
GGCATCGCTG GACCAGATGG GCTTCCTGGC AGGGACGGGC AAGCAGGACA GCAGGGGGAG 
CAGGGAGACG ATGGGGACCC TGGCCCCATG GGCCCTGCTG GGAAGAGAGG AAATCCAGGT 
GTGGCCGGCT TACCTGGAGC ACAGGGACCC CCAGGATTCA AGGGTGAGAG TGGGTTACCC 
GGACAGCTGG GTCCCCCTGG CAAGCGAGGA ACAGAGGGCA GAACGGGGCT CCCTGGAAAC 
CAGGGGGAGC CTGGGTCCAA AGGCCAGCCG GGCGACTCTG GCGAGATGGG CTTCCCAGGA 
ATGGCAGGTC TCTTCGGACC CAAGGGCCCG CCTGGAGACA TTGGCTTCAA AGGCATCCAG 
GGCCCTCGGG GGCCACCTGG CTTGATGGGA AAGGAAGGCA TCGTCGGGCC CCTCGGAATC 
CTGGGACCTT CGGGACTCCC GGGTCCGAAG GGTGACAAAG GCAGCCGTGG GGACTGGGGA 
TTGCAAGGTC CGAGGGGTCC TCCCGGCCCC AGAGGGCGGC CCGGCCCCCC GGGTCCTCCA 
GGGGGTCCTA TCCAATTGCA ACAAGATGAT CTTGGGGCAG CTTTCCAGAC GTGGATGGAC 
ACCAGTGGAG CACTCAGGCC AGAGAGTTAC AGCTATCCAG ACCGGCTGGT GCTGGACCAG 
GGAGGAGAGA TCTTTAAAAC CTTACACTAC CTCAGCAACC TCATCCAGAG CATTAAGACG 
CCCCTGGGCA CCAAAGAGAA CCCCGCCCGG GTCTGCAGGG ACCTCATGGA CTGTGAGCAG 
AAGATGGTGG ATGGTACCTA CTGGGTGGAT CCAAACCTTG GCTGCTCCTC TGACACCATC 
GAGGTCTCCT GCAACTTCAC TCATGGTGGA CAGACGTGTC TCAAGCCCAT CACGGCCTCC 
AAGGTCGAGT TTGCCATCAG CCGGGTCCAG ATGAATTTCC TGCACCTGCT AAGCTCCGAG 
GTGACCCAGC ACATCACCAT CCACTGCCTT AACATGACCG TGTGGCAGGA GGGCACTGGG 
CAGACCCCAG CCAAGCAGGC CGTACGCTTC CGGGCCTGGA ATGGACAGAT TTTTGAAGCT 
GGGGGTCAGT TCCGGCCCGA GGTGTCCATG GATGGCTGCA AGGTCCAAGA TGGCCGCTGG 
CATCAGACAC TCTTCACCTT CCGGACCCAA GACCCCCAAC AGCTGCCCAT CATCAGTGTG 
GACAACCTCC CTCCTGCCTC ATCAGGGAAG CAGTACCGCC TGGAAGTTGG ACCTGCGTGC 
TTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_006717310.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717373.1 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X12, mRNA.

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CloneID	OHu43566
Clone ID Related Accession (Same CDS sequence)	XM_006717310.3
Accession Version	XM_006717310.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717310.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG AGGCCTGCCT GGCGTTCCTG GCAAGAGGGG CAAGATGGGG GTTTCCTGGA 
GTCTTTGGGG AAAGAGGCCC TCCTGGACTG GATGGAAATC CTGGAGAACT GGGCCTGCCA 
GGCCCCCCTG GAGTCCCCGG CCTCATTGGT GACTTAGGAG TGTTGGGTCC GATTGGCTAC 
CCGGGACCCA AGGGCATGAA GGGACTGATG GGCAGCGTGG GGGAGCCCGG ACTGAAAGGT 
GATAAGGGTG AACAAGGGGT TCCAGGTGTG TCAGGAGATC CCGGATTCCA AGGAGACAAG 
GGGAGCCAGG GGTTGCCAGG GTTCCCCGGT GCACGGGGGA AGCCAGGGCC TCTGGGCAAA 
GTCGGAGACA AAGGATCCAT TGGGTTTCCC GGGCCCCCTG GACCCGAGGG ATTCCCAGGA 
GACATCGGCC CCCCTGGCGA CAATGGCCCA GAAGGCATGA AGGGTAAGCC TGGAGCCCGA 
GGCCTGCCGG GACCCCGTGG GCAGCTGGGG CCCGAGGGAG ATGAGGGACC CATGGGGCCG 
CCAGGGGCCC CTGGCTTGGA GGGTCAGCCT GGCAGGAAGG GGTTTCCTGG GAGGCCCGGC 
CTGGATGGCG TGAAGGGGGA ACCAGGGGAT CCTGGTCGGC CGGGGCCTGT GGGAGAGCAG 
GGATTTATGG GATTCATTGG TCTGGTCGGG GAGCCAGGAA TCGTGGGAGA AAAGGGTGAT 
CGTGGCATGA TGGGACCCCC AGGCGTGCCT GGACCCAAGG GGTCGATGGG TCATCCTGGA 
ATGCCAGGTG GTATGGGGAC CCCTGGAGAG CCTGGACCCC AGGGTCCTCC AGGATCTCGA 
GGCCCACCAG GCATGAGGGG AGCAAAGGGA CGTCGGGGCC CCCGAGGACC GGACGGACCA 
GCTGGGGAGC AAGGGTCCAG GGGCCTGAAG GGCCCTCCAG GACCCCAGGG CAGACCGGGC 
CGGCCTGGAC AGCAGGGTGT GGCTGGTGAG CGAGGCCACT TGGGCTCGAG AGGCTTTCCT 
GGCATCCCGG GTCCCTCAGG CCCCCCAGGC ACCAAGGGCC TCCCAGGAGA ACCGGGCCCT 
CAGGGACCCC AGGGGCCAAT TGGGCCTCCA GGAGAGATGG GACCCAAGGG GCCGCCTGGT 
GCAGTGGGAG AACCGGGCCT TCCTGGGGAA GCCGGGATGA AGGGTGACCT TGGACCCCTG 
GGCACTCCTG GGGAGCAGGG CCTCATTGGG CAACGGGGAG AGCCAGGCCT TGAGGGTGAC 
AGTGGCCCCA TGGGACCTGA TGGGCTGAAG GGGGACAGGG GAGACCCAGG GCCTGATGGA 
GAACATGGCG AGAAAGGCCA GGAAGGGCTG ATGGGTGAGG ACGGGCCCCC CGGCCCCCCT 
GGCGTCACTG GTGTCCGGGG TCCTGAAGGA AAATCAGGGA AGCAAGGCGA GAAGGGCCGC 
ACTGGAGCCA AGGGTGCCAA GGGCTATCAA GGACAGCTGG GTGAGATGGG CGTCCCTGGA 
GACCCTGGAC CCCCTGGCAC TCCAGGCCCT AAAGGGTCCC GGGGCAGCCT GGGACCAACG 
GGTGCTCCGG GACGCATGGG GGCCCAAGGA GAACCGGGAC TGGCTGGTTA TGATGGACAC 
AAAGGCATTG TGGGACCCCT TGGACCTCCT GGACCAAAAG GCGAAAAGGG GGAGCAGGGC 
GAGGACGGCA AGGCTGAGGG GCCCCCTGGG CCACCTGGAG ATCGGGGCCC TGTGGGTGAT 
CGAGGAGACC GCGGGGAACC GGGAGACCCT GGGTACCCTG GACAGGAGGG TGTGCAAGGC 
CTCCGTGGAA AGCCAGGCCA GCAGGGCCAA CCCGGGCATC CGGGACCCCG GGGGTGGCCG 
GGACCCAAAG GATCGAAAGG CGCAGAGGGA CCAAAGGGAA AGCAAGGCAA GGCAGGGGCC 
CCAGGCCGGA GGGGGGTCCA GGGCCTGCAG GGGCTGCCAG GGCCCCGGGG CGTGGTGGGG 
AGACAGGGCC TCGAGGGCAT CGCTGGACCA GATGGGCTTC CTGGCAGGGA CGGGCAAGCA 
GGACAGCAGG GGGAGCAGGG AGACGATGGG GACCCTGGCC CCATGGGCCC TGCTGGGAAG 
AGAGGAAATC CAGGTGTGGC CGGCTTACCT GGAGCACAGG GACCCCCAGG ATTCAAGGGT 
GAGAGTGGGT TACCCGGACA GCTGGGTCCC CCTGGCAAGC GAGGAACAGA GGGCAGAACG 
GGGCTCCCTG GAAACCAGGG GGAGCCTGGG TCCAAAGGCC AGCCGGGCGA CTCTGGCGAG 
ATGGGCTTCC CAGGAATGGC AGGTCTCTTC GGACCCAAGG GCCCGCCTGG AGACATTGGC 
TTCAAAGGCA TCCAGGGCCC TCGGGGGCCA CCTGGCTTGA TGGGAAAGGA AGGCATCGTC 
GGGCCCCTCG GAATCCTGGG ACCTTCGGGA CTCCCGGGTC CGAAGGGTGA CAAAGGCAGC 
CGTGGGGACT GGGGATTGCA AGGTCCGAGG GGTCCTCCCG GCCCCAGAGG GCGGCCCGGC 
CCCCCGGGTC CTCCAGGGGG TCCTATCCAA TTGCAACAAG ATGATCTTGG GGCAGCTTTC 
CAGACGTGGA TGGACACCAG TGGAGCACTC AGGCCAGAGA GTTACAGCTA TCCAGACCGG 
CTGGTGCTGG ACCAGGGAGG AGAGATCTTT AAAACCTTAC ACTACCTCAG CAACCTCATC 
CAGAGCATTA AGACGCCCCT GGGCACCAAA GAGAACCCCG CCCGGGTCTG CAGGGACCTC 
ATGGACTGTG AGCAGAAGAT GGTGGATGGT ACCTACTGGG TGGATCCAAA CCTTGGCTGC 
TCCTCTGACA CCATCGAGGT CTCCTGCAAC TTCACTCATG GTGGACAGAC GTGTCTCAAG 
CCCATCACGG CCTCCAAGGT CGAGTTTGCC ATCAGCCGGG TCCAGATGAA TTTCCTGCAC 
CTGCTAAGCT CCGAGGTGAC CCAGCACATC ACCATCCACT GCCTTAACAT GACCGTGTGG 
CAGGAGGGCA CTGGGCAGAC CCCAGCCAAG CAGGCCGTAC GCTTCCGGGC CTGGAATGGA 
CAGATTTTTG AAGCTGGGGG TCAGTTCCGG CCCGAGGTGT CCATGGATGG CTGCAAGGTC 
CAAGATGGCC GCTGGCATCA GACACTCTTC ACCTTCCGGA CCCAAGACCC CCAACAGCTG 
CCCATCATCA GTGTGGACAA CCTCCCTCCT GCCTCATCAG GGAAGCAGTA CCGCCTGGAA 
GTTGGACCTG CGTGCTTCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717373.1
CDS	539..3802
Translation	MENEACLAFLARGARWGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_006717310.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717310.3

ORF Insert Sequence:

ATGGAGAACG AGGCCTGCCT GGCGTTCCTG GCAAGAGGGG CAAGATGGGG GTTTCCTGGA 
GTCTTTGGGG AAAGAGGCCC TCCTGGACTG GATGGAAATC CTGGAGAACT GGGCCTGCCA 
GGCCCCCCTG GAGTCCCCGG CCTCATTGGT GACTTAGGAG TGTTGGGTCC GATTGGCTAC 
CCGGGACCCA AGGGCATGAA GGGACTGATG GGCAGCGTGG GGGAGCCCGG ACTGAAAGGT 
GATAAGGGTG AACAAGGGGT TCCAGGTGTG TCAGGAGATC CCGGATTCCA AGGAGACAAG 
GGGAGCCAGG GGTTGCCAGG GTTCCCCGGT GCACGGGGGA AGCCAGGGCC TCTGGGCAAA 
GTCGGAGACA AAGGATCCAT TGGGTTTCCC GGGCCCCCTG GACCCGAGGG ATTCCCAGGA 
GACATCGGCC CCCCTGGCGA CAATGGCCCA GAAGGCATGA AGGGTAAGCC TGGAGCCCGA 
GGCCTGCCGG GACCCCGTGG GCAGCTGGGG CCCGAGGGAG ATGAGGGACC CATGGGGCCG 
CCAGGGGCCC CTGGCTTGGA GGGTCAGCCT GGCAGGAAGG GGTTTCCTGG GAGGCCCGGC 
CTGGATGGCG TGAAGGGGGA ACCAGGGGAT CCTGGTCGGC CGGGGCCTGT GGGAGAGCAG 
GGATTTATGG GATTCATTGG TCTGGTCGGG GAGCCAGGAA TCGTGGGAGA AAAGGGTGAT 
CGTGGCATGA TGGGACCCCC AGGCGTGCCT GGACCCAAGG GGTCGATGGG TCATCCTGGA 
ATGCCAGGTG GTATGGGGAC CCCTGGAGAG CCTGGACCCC AGGGTCCTCC AGGATCTCGA 
GGCCCACCAG GCATGAGGGG AGCAAAGGGA CGTCGGGGCC CCCGAGGACC GGACGGACCA 
GCTGGGGAGC AAGGGTCCAG GGGCCTGAAG GGCCCTCCAG GACCCCAGGG CAGACCGGGC 
CGGCCTGGAC AGCAGGGTGT GGCTGGTGAG CGAGGCCACT TGGGCTCGAG AGGCTTTCCT 
GGCATCCCGG GTCCCTCAGG CCCCCCAGGC ACCAAGGGCC TCCCAGGAGA ACCGGGCCCT 
CAGGGACCCC AGGGGCCAAT TGGGCCTCCA GGAGAGATGG GACCCAAGGG GCCGCCTGGT 
GCAGTGGGAG AACCGGGCCT TCCTGGGGAA GCCGGGATGA AGGGTGACCT TGGACCCCTG 
GGCACTCCTG GGGAGCAGGG CCTCATTGGG CAACGGGGAG AGCCAGGCCT TGAGGGTGAC 
AGTGGCCCCA TGGGACCTGA TGGGCTGAAG GGGGACAGGG GAGACCCAGG GCCTGATGGA 
GAACATGGCG AGAAAGGCCA GGAAGGGCTG ATGGGTGAGG ACGGGCCCCC CGGCCCCCCT 
GGCGTCACTG GTGTCCGGGG TCCTGAAGGA AAATCAGGGA AGCAAGGCGA GAAGGGCCGC 
ACTGGAGCCA AGGGTGCCAA GGGCTATCAA GGACAGCTGG GTGAGATGGG CGTCCCTGGA 
GACCCTGGAC CCCCTGGCAC TCCAGGCCCT AAAGGGTCCC GGGGCAGCCT GGGACCAACG 
GGTGCTCCGG GACGCATGGG GGCCCAAGGA GAACCGGGAC TGGCTGGTTA TGATGGACAC 
AAAGGCATTG TGGGACCCCT TGGACCTCCT GGACCAAAAG GCGAAAAGGG GGAGCAGGGC 
GAGGACGGCA AGGCTGAGGG GCCCCCTGGG CCACCTGGAG ATCGGGGCCC TGTGGGTGAT 
CGAGGAGACC GCGGGGAACC GGGAGACCCT GGGTACCCTG GACAGGAGGG TGTGCAAGGC 
CTCCGTGGAA AGCCAGGCCA GCAGGGCCAA CCCGGGCATC CGGGACCCCG GGGGTGGCCG 
GGACCCAAAG GATCGAAAGG CGCAGAGGGA CCAAAGGGAA AGCAAGGCAA GGCAGGGGCC 
CCAGGCCGGA GGGGGGTCCA GGGCCTGCAG GGGCTGCCAG GGCCCCGGGG CGTGGTGGGG 
AGACAGGGCC TCGAGGGCAT CGCTGGACCA GATGGGCTTC CTGGCAGGGA CGGGCAAGCA 
GGACAGCAGG GGGAGCAGGG AGACGATGGG GACCCTGGCC CCATGGGCCC TGCTGGGAAG 
AGAGGAAATC CAGGTGTGGC CGGCTTACCT GGAGCACAGG GACCCCCAGG ATTCAAGGGT 
GAGAGTGGGT TACCCGGACA GCTGGGTCCC CCTGGCAAGC GAGGAACAGA GGGCAGAACG 
GGGCTCCCTG GAAACCAGGG GGAGCCTGGG TCCAAAGGCC AGCCGGGCGA CTCTGGCGAG 
ATGGGCTTCC CAGGAATGGC AGGTCTCTTC GGACCCAAGG GCCCGCCTGG AGACATTGGC 
TTCAAAGGCA TCCAGGGCCC TCGGGGGCCA CCTGGCTTGA TGGGAAAGGA AGGCATCGTC 
GGGCCCCTCG GAATCCTGGG ACCTTCGGGA CTCCCGGGTC CGAAGGGTGA CAAAGGCAGC 
CGTGGGGACT GGGGATTGCA AGGTCCGAGG GGTCCTCCCG GCCCCAGAGG GCGGCCCGGC 
CCCCCGGGTC CTCCAGGGGG TCCTATCCAA TTGCAACAAG ATGATCTTGG GGCAGCTTTC 
CAGACGTGGA TGGACACCAG TGGAGCACTC AGGCCAGAGA GTTACAGCTA TCCAGACCGG 
CTGGTGCTGG ACCAGGGAGG AGAGATCTTT AAAACCTTAC ACTACCTCAG CAACCTCATC 
CAGAGCATTA AGACGCCCCT GGGCACCAAA GAGAACCCCG CCCGGGTCTG CAGGGACCTC 
ATGGACTGTG AGCAGAAGAT GGTGGATGGT ACCTACTGGG TGGATCCAAA CCTTGGCTGC 
TCCTCTGACA CCATCGAGGT CTCCTGCAAC TTCACTCATG GTGGACAGAC GTGTCTCAAG 
CCCATCACGG CCTCCAAGGT CGAGTTTGCC ATCAGCCGGG TCCAGATGAA TTTCCTGCAC 
CTGCTAAGCT CCGAGGTGAC CCAGCACATC ACCATCCACT GCCTTAACAT GACCGTGTGG 
CAGGAGGGCA CTGGGCAGAC CCCAGCCAAG CAGGCCGTAC GCTTCCGGGC CTGGAATGGA 
CAGATTTTTG AAGCTGGGGG TCAGTTCCGG CCCGAGGTGT CCATGGATGG CTGCAAGGTC 
CAAGATGGCC GCTGGCATCA GACACTCTTC ACCTTCCGGA CCCAAGACCC CCAACAGCTG 
CCCATCATCA GTGTGGACAA CCTCCCTCCT GCCTCATCAG GGAAGCAGTA CCGCCTGGAA 
GTTGGACCTG CGTGCTTCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_011519138.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517440.1 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

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CloneID	OHu73932
Clone ID Related Accession (Same CDS sequence)	XM_011519138.2
Accession Version	XM_011519138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTAG GCGCCGGAGG AAGGACCGAC CGGCCAGCCT CGCACGTAGG GACCCAGGGG 
TTTCTCTTCT CCTGGATCTT AGTCTCGTTT GCCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGACATCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGAGCCCT 
GCACCCCCGG GAGTCATTCC TTTCCAGTCG GGCTTCATCT TTACGCAGCG GGCCCGGCTC 
CAGGCTCCCA CGGGCACCGT CATTCCTGCC GCCTTGGGCA CAGAGCTGGC ACTGGTGCTG 
AGCCTCTGCT CCCACCGGGT GAACCATGCC TTCCTCTTCG CTGTCCGCAG CCAGAAACGC 
AAGCTGCAGC TGGGCCTGCA GTTCCTCCCC GGCAAGACGG TCGTCCACCT CGGGTCCCGG 
CGCTCAGTGG CCTTCGACCT CGACATGCAC GACGGGCGCT GGCACCACCT GGCCCTCGAG 
CTCCGAGGCC GCACAGTCAC TCTGGTGACT GCCTGCGGGC AGCGCCGGGT GCCTGTCCTG 
CTGCCTTTCC ACAGGGACCC TGCACTCGAC CCTGGGGGCT CCTTCCTCTT TGGGAAGATG 
AACCCGCATG CAGTCCAGTT TGAAGGTGCT CTCTGCCAGT TCAGTATCTA CCCTGTGACG 
CAGGTCGCTC ACAATTACTG TACCCACCTG AGGAAGCAGT GTGGACAGGC TGACACGTAC 
CAGTCCCCAC TGGGACCTCT CTTCTCCCAA GACTCTGGCA GACCTTTTAC CTTCCAGTCC 
GACCTCGCCC TGCTAGGCCT GGAGAACTTG ACCACTGCCA CACCAGCCCT GGGGTCACTG 
CCAGCAGGCA GGGGACCCAG GGGGACTGTG GCACCCGCCA CGCCCACCAA GCCCCAAAGG 
ACTAGCCCCA CAAACCCTCA CCAGCATATG GCGGTGGGAG GCCCAGCCCA AACCCCGCTG 
CTACCTGCCA AGCTGTCAGC CAGTAACGCA CTTGATCCCA TGCTCCCAGC CTCTGTTGGC 
GGCTCTACCA GAACGCCTCG CCCTGCGGCC GCTCAACCAT CACAGAAGAT CACAGCCACC 
AAAATCCCCA AAAGCCTCCC TACCAAGCCT TCGGCCCCTT CTACTTCAAT TGTGCCCATC 
AAAAGCCCCC ATCCTACCCA GAAAACAGCT CCATCTTCAT TTACAAAGTC AGCCCTACCC 
ACTCAGAAGC AAGTGCCACC TACTTCCCGT CCAGTTCCTG CCAGAGTCTC CCGTCCCGCA 
GAGAAGCCCA TCCAGAGGAA CCCGGGAATG CCCAGGCCCC CACCGCCCAG CACCCGGCCC 
CTACCTCCTA CCACCAGCTC CTCTAAAAAA CCCATTCCCA CACTAGCTCG GACTGAGGCC 
AAGATAACCA GCCATGCCAG TAAGCCGGCC TCTGCCCGCA CCAGCACCCA CAAACCTCCC 
CCATTTACTG CTTTATCCTC ATCTCCTGCC CCTACTCCTG GTTCTACCAG GAGTACTCGG 
CCACCAGCCA CGATGGTACC TCCAACTTCG GGCACCAGCA CTCCCAGAAC AGCACCTGCC 
GTCCCCACTC CTGGCTCAGC TCCCACTGGA AGCAAGAAGC CCATTGGATC GGAAGCCTCA 
AAGAAAGCCG GACCCAAGAG CAGCCCCCGG AAGCCTGTCC CCCTCAGACC TGGGAAGGCA 
GCCAGGGATG TCCCCTTGAG CGATCTGACA ACCAGGCCTA GCCCCAGACA GCCCCAGCCC 
AGTCAGCAGA CCACCCCGGC CCTGGTATTG GCCCCGGCGC AATTCCTGTC CTCCAGCCCC 
CGGCCCACGA GCAGTGGCTA TTCGATCTTC CACCTGGCAG GATCTACGCC TTTCCCTCTG 
CTGATGGGGC CTCCGGGACC CAAGGGAGAC TGTGGCTTGC CGGGTCCCCC TGGGCTACCT 
GGGCTACCTG GAATCCCTGG TGCACGTGGG CCTCGGGGTC CTCCTGGGCC TTATGGAAAT 
CCAGGTCTCC CCGGCCCTCC TGGAGCCAAA GGACAGAAAG GGGACCCAGG GCTCTCACCA 
GGAAAGGCCC ACGATGGGGC AAAGGGTGAC ATGGGCTTGC CTGGGCTCTC CGGGAATCCA 
GGACCTCCGG GACGAAAGGG ACACAAGGGC TATCCTGGAC CGGCAGGGCA CCCCGGAGAA 
CAGGGGCAGC CAGGACCTGA GGGCAGCCCA GGGGCCAAAG GTTACCCTGG CAGGCAGGGG 
TTACCTGGAC CGGTAGGAGA TCCCGGCCCC AAAGGCAGCA GGGGCTACAT TGGGCTCCCA 
GGGCTCTTCG GCCTGCCAGG GTCTGATGGA GAACGAGGCC TGCCTGGCGT TCCTGGCAAG 
AGGGGCAAGA TGGGTATGCC GGGGTTTCCT GGAGTCTTTG GGGAAAGAGG CCCTCCTGGA 
CTGGATGGAA ATCCTGGAGA ACTGGGCCTG CCAGGCCCCC CTGGAGTCCC CGGCCTCATT 
GGTGACTTAG GAGTGTTGGG TCCGATTGGC TACCCGGGAC CCAAGGGCAT GAAGGGACTG 
ATGGGCAGCG TGGGGGAGCC CGGACTGAAA GGTGATAAGG GTGAACAAGG GGTTCCAGGT 
GTGTCAGGAG ATCCCGGATT CCAAGGAGAC AAGGGGAGCC AGGGGTTGCC AGGGTTCCCC 
GGTGCACGGG GGAAGCCAGG GCCTCTGGGC AAAGTCGGAG ACAAAGGATC CATTGGGTTT 
CCCGGGCCCC CTGGACCCGA GGGATTCCCA GGAGACATCG GCCCCCCTGG CGACAATGGC 
CCAGAAGGCA TGAAGGGTAA GCCTGGAGCC CGAGGCCTGC CGGGACCCCG TGGGCAGCTG 
GGGCCCGAGG GAGATGAGGG ACCCATGGGG CCGCCAGGGG CCCCTGGCTT GGAGGGTCAG 
CCTGGCAGGA AGGGGTTTCC TGGGAGGCCC GGCCTGGATG GCGTGAAGGG GGAACCAGGG 
GATCCTGGTC GGCCGGGGCC TGTGGGAGAG CAGGGATTTA TGGGATTCAT TGGTCTGGTC 
GGGGAGCCAG GAATCGTGGG AGAAAAGGGT GATCGTGGCA TGATGGGACC CCCAGGCGTG 
CCTGGACCCA AGGGGTCGAT GGGTCATCCT GGAATGCCAG GTGGTATGGG GACCCCTGGA 
GAGCCTGGAC CCCAGGGTCC TCCAGGATCT CGAGGCCCAC CAGGCATGAG GGGAGCAAAG 
GGACGTCGGG GCCCCCGAGG ACCGGACGGA CCAGCTGGGG AGCAAGGGTC CAGGGGCCTG 
AAGGGCCCTC CAGGACCCCA GGGCAGACCG GGCCGGCCTG GACAGCAGGG TGTGGCTGGT 
GAGCGAGGCC ACTTGGGCTC GAGAGGCTTT CCTGGCATCC CGGGTCCCTC AGGCCCCCCA 
GGCACCAAGG GCCTCCCAGG AGAACCGGGC CCTCAGGGAC CCCAGGGGCC AATTGGGCCT 
CCAGGAGAGA TGGGACCCAA GGGGCCGCCT GGTGCAGTGG GAGAACCGGG CCTTCCTGGG 
GAAGCCGGGA TGAAGGGTGA CCTTGGACCC CTGGGCACTC CTGGGGAGCA GGGCCTCATT 
GGGCAACGGG GAGAGCCAGG CCTTGAGGGT GACAGTGGCC CCATGGGACC TGATGGGCTG 
AAGGGGGACA GGGGAGACCC AGGGCCTGAT GGAGAACATG GCGAGAAAGG CCAGGAAGGG 
CTGATGGGTG AGGACGGGCC CCCCGGCCCC CCTGGCGTCA CTGGTGTCCG GGGTCCTGAA 
GGAAAATCAG GGAAGCAAGG CGAGAAGGGC CGCACTGGAG CCAAGGGTGC CAAGGGCTAT 
CAAGGACAGC TGGGTGAGAT GGGCGTCCCT GGAGACCCTG GACCCCCTGG CACTCCAGGC 
CCTAAAGGGT CCCGGGGCAG CCTGGGACCA ACGGGTGCTC CGGGACGCAT GGGGGCCCAA 
GGAGAACCGG GACTGGCTGG TTATGATGGA CACAAAGGCA TTGTGGGACC CCTTGGACCT 
CCTGGACCAA AAGGCGAAAA GGGGGAGCAG GGCGAGGACG GCAAGGCTGA GGGGCCCCCT 
GGGCCACCTG GAGATCGGGG CCCTGTGGGT GATCGAGGAG ACCGCGGGGA ACCGGGAGAC 
CCTGGGTACC CTGGACAGGA GGGTGTGCAA GGCCTCCGTG GAAAGCCAGG CCAGCAGGGC 
CAACCCGGGC ATCCGGGACC CCGGGGGTGG CCGGGACCCA AAGGATCGAA AGGCGCAGAG 
GGACCAAAGG GAAAGCAAGG CAAGGCAGGG GCCCCAGGCC GGAGGGGGGT CCAGGGCCTG 
CAGGGGCTGC CAGGGCCCCG GGGCGTGGTG GGGAGACAGG GCCTCGAGGG CATCGCTGGA 
CCAGATGGGC TTCCTGGCAG GGACGGGCAA GCAGGACAGC AGGGGGAGCA GGGAGACGAT 
GGGGACCCTG GCCCCATGGG CCCTGCTGGG AAGAGAGGAA ATCCAGGTGT GGCCGGCTTA 
CCTGGAGCAC AGGGACCCCC AGGATTCAAG GGTGAGAGTG GGTTACCCGG ACAGCTGGGT 
CCCCCTGGCA AGCGAGGAAC AGAGGGCAGA ACGGGGCTCC CTGGAAACCA GGGGGAGCCT 
GGGTCCAAAG GCCAGCCGGG CGACTCTGGC GAGATGGGCT TCCCAGGAAT GGCAGGTCTC 
TTCGGACCCA AGGGCCCGCC TGGAGACATT GGCTTCAAAG GCATCCAGGG CCCTCGGGGG 
CCACCTGGCT TGATGGGAAA GGAAGGCATC GTCGGGCCCC TCGGAATCCT GGGACCTTCG 
GGACTCCCGG GTCCGAAGGG TGACAAAGGC AGCCGTGGGG ACTGGGGATT GCAAGGTCCG 
AGGGGTCCTC CCGGCCCCAG AGGGCGGCCC GGCCCCCCGG GTCCTCCAGG GGGTCCTATC 
CAATTGCAAC AAGATGATCT TGGGGCAGCT TTCCAGACGT GGATGGACAC CAGTGGAGCA 
CTCAGGCCAG AGAGTTACAG CTATCCAGAC CGGCTGGTGC TGGACCAGGG AGGAGAGATC 
TTTAAAACCT TACACTACCT CAGCAACCTC ATCCAGAGCA TTAAGACGCC CCTGGGCACC 
AAAGAGAACC CCGCCCGGGT CTGCAGGGAC CTCATGGACT GTGAGCAGAA GATGGTGGAT 
GGTACCTACT GGGTGGATCC AAACCTTGGC TGCTCCTCTG ACACCATCGA GGTCTCCTGC 
AACTTCACTC ATGGTGGACA GACGTGTCTC AAGCCCATCA CGGCCTCCAA GGTCGAGTTT 
GCCATCAGCC GGGTCCAGAT GAATTTCCTG CACCTGCTAA GCTCCGAGGT GACCCAGCAC 
ATCACCATCC ACTGCCTTAA CATGACCGTG TGGCAGGAGG GCACTGGGCA GACCCCAGCC 
AAGCAGGCCG TACGCTTCCG GGCCTGGAAT GGACAGATTT TTGAAGCTGG GGGTCAGTTC 
CGGCCCGAGG TGTCCATGGA TGGCTGCAAG GTCCAAGATG GCCGCTGGCA TCAGACACTC 
TTCACCTTCC GGACCCAAGA CCCCCAACAG CTGCCCATCA TCAGTGTGGA CAACCTCCCT 
CCTGCCTCAT CAGGGAAGCA GTACCGCCTG GAAGTTGGAC CTGCGTGCTT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517440.1
CDS	45..5621
Translation	MPLGAGGRTDRPASHVGTQGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_011519138.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519138.2

ORF Insert Sequence:

ATGCCGTTAG GCGCCGGAGG AAGGACCGAC CGGCCAGCCT CGCACGTAGG GACCCAGGGG 
TTTCTCTTCT CCTGGATCTT AGTCTCGTTT GCCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGACATCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGAGCCCT 
GCACCCCCGG GAGTCATTCC TTTCCAGTCG GGCTTCATCT TTACGCAGCG GGCCCGGCTC 
CAGGCTCCCA CGGGCACCGT CATTCCTGCC GCCTTGGGCA CAGAGCTGGC ACTGGTGCTG 
AGCCTCTGCT CCCACCGGGT GAACCATGCC TTCCTCTTCG CTGTCCGCAG CCAGAAACGC 
AAGCTGCAGC TGGGCCTGCA GTTCCTCCCC GGCAAGACGG TCGTCCACCT CGGGTCCCGG 
CGCTCAGTGG CCTTCGACCT CGACATGCAC GACGGGCGCT GGCACCACCT GGCCCTCGAG 
CTCCGAGGCC GCACAGTCAC TCTGGTGACT GCCTGCGGGC AGCGCCGGGT GCCTGTCCTG 
CTGCCTTTCC ACAGGGACCC TGCACTCGAC CCTGGGGGCT CCTTCCTCTT TGGGAAGATG 
AACCCGCATG CAGTCCAGTT TGAAGGTGCT CTCTGCCAGT TCAGTATCTA CCCTGTGACG 
CAGGTCGCTC ACAATTACTG TACCCACCTG AGGAAGCAGT GTGGACAGGC TGACACGTAC 
CAGTCCCCAC TGGGACCTCT CTTCTCCCAA GACTCTGGCA GACCTTTTAC CTTCCAGTCC 
GACCTCGCCC TGCTAGGCCT GGAGAACTTG ACCACTGCCA CACCAGCCCT GGGGTCACTG 
CCAGCAGGCA GGGGACCCAG GGGGACTGTG GCACCCGCCA CGCCCACCAA GCCCCAAAGG 
ACTAGCCCCA CAAACCCTCA CCAGCATATG GCGGTGGGAG GCCCAGCCCA AACCCCGCTG 
CTACCTGCCA AGCTGTCAGC CAGTAACGCA CTTGATCCCA TGCTCCCAGC CTCTGTTGGC 
GGCTCTACCA GAACGCCTCG CCCTGCGGCC GCTCAACCAT CACAGAAGAT CACAGCCACC 
AAAATCCCCA AAAGCCTCCC TACCAAGCCT TCGGCCCCTT CTACTTCAAT TGTGCCCATC 
AAAAGCCCCC ATCCTACCCA GAAAACAGCT CCATCTTCAT TTACAAAGTC AGCCCTACCC 
ACTCAGAAGC AAGTGCCACC TACTTCCCGT CCAGTTCCTG CCAGAGTCTC CCGTCCCGCA 
GAGAAGCCCA TCCAGAGGAA CCCGGGAATG CCCAGGCCCC CACCGCCCAG CACCCGGCCC 
CTACCTCCTA CCACCAGCTC CTCTAAAAAA CCCATTCCCA CACTAGCTCG GACTGAGGCC 
AAGATAACCA GCCATGCCAG TAAGCCGGCC TCTGCCCGCA CCAGCACCCA CAAACCTCCC 
CCATTTACTG CTTTATCCTC ATCTCCTGCC CCTACTCCTG GTTCTACCAG GAGTACTCGG 
CCACCAGCCA CGATGGTACC TCCAACTTCG GGCACCAGCA CTCCCAGAAC AGCACCTGCC 
GTCCCCACTC CTGGCTCAGC TCCCACTGGA AGCAAGAAGC CCATTGGATC GGAAGCCTCA 
AAGAAAGCCG GACCCAAGAG CAGCCCCCGG AAGCCTGTCC CCCTCAGACC TGGGAAGGCA 
GCCAGGGATG TCCCCTTGAG CGATCTGACA ACCAGGCCTA GCCCCAGACA GCCCCAGCCC 
AGTCAGCAGA CCACCCCGGC CCTGGTATTG GCCCCGGCGC AATTCCTGTC CTCCAGCCCC 
CGGCCCACGA GCAGTGGCTA TTCGATCTTC CACCTGGCAG GATCTACGCC TTTCCCTCTG 
CTGATGGGGC CTCCGGGACC CAAGGGAGAC TGTGGCTTGC CGGGTCCCCC TGGGCTACCT 
GGGCTACCTG GAATCCCTGG TGCACGTGGG CCTCGGGGTC CTCCTGGGCC TTATGGAAAT 
CCAGGTCTCC CCGGCCCTCC TGGAGCCAAA GGACAGAAAG GGGACCCAGG GCTCTCACCA 
GGAAAGGCCC ACGATGGGGC AAAGGGTGAC ATGGGCTTGC CTGGGCTCTC CGGGAATCCA 
GGACCTCCGG GACGAAAGGG ACACAAGGGC TATCCTGGAC CGGCAGGGCA CCCCGGAGAA 
CAGGGGCAGC CAGGACCTGA GGGCAGCCCA GGGGCCAAAG GTTACCCTGG CAGGCAGGGG 
TTACCTGGAC CGGTAGGAGA TCCCGGCCCC AAAGGCAGCA GGGGCTACAT TGGGCTCCCA 
GGGCTCTTCG GCCTGCCAGG GTCTGATGGA GAACGAGGCC TGCCTGGCGT TCCTGGCAAG 
AGGGGCAAGA TGGGTATGCC GGGGTTTCCT GGAGTCTTTG GGGAAAGAGG CCCTCCTGGA 
CTGGATGGAA ATCCTGGAGA ACTGGGCCTG CCAGGCCCCC CTGGAGTCCC CGGCCTCATT 
GGTGACTTAG GAGTGTTGGG TCCGATTGGC TACCCGGGAC CCAAGGGCAT GAAGGGACTG 
ATGGGCAGCG TGGGGGAGCC CGGACTGAAA GGTGATAAGG GTGAACAAGG GGTTCCAGGT 
GTGTCAGGAG ATCCCGGATT CCAAGGAGAC AAGGGGAGCC AGGGGTTGCC AGGGTTCCCC 
GGTGCACGGG GGAAGCCAGG GCCTCTGGGC AAAGTCGGAG ACAAAGGATC CATTGGGTTT 
CCCGGGCCCC CTGGACCCGA GGGATTCCCA GGAGACATCG GCCCCCCTGG CGACAATGGC 
CCAGAAGGCA TGAAGGGTAA GCCTGGAGCC CGAGGCCTGC CGGGACCCCG TGGGCAGCTG 
GGGCCCGAGG GAGATGAGGG ACCCATGGGG CCGCCAGGGG CCCCTGGCTT GGAGGGTCAG 
CCTGGCAGGA AGGGGTTTCC TGGGAGGCCC GGCCTGGATG GCGTGAAGGG GGAACCAGGG 
GATCCTGGTC GGCCGGGGCC TGTGGGAGAG CAGGGATTTA TGGGATTCAT TGGTCTGGTC 
GGGGAGCCAG GAATCGTGGG AGAAAAGGGT GATCGTGGCA TGATGGGACC CCCAGGCGTG 
CCTGGACCCA AGGGGTCGAT GGGTCATCCT GGAATGCCAG GTGGTATGGG GACCCCTGGA 
GAGCCTGGAC CCCAGGGTCC TCCAGGATCT CGAGGCCCAC CAGGCATGAG GGGAGCAAAG 
GGACGTCGGG GCCCCCGAGG ACCGGACGGA CCAGCTGGGG AGCAAGGGTC CAGGGGCCTG 
AAGGGCCCTC CAGGACCCCA GGGCAGACCG GGCCGGCCTG GACAGCAGGG TGTGGCTGGT 
GAGCGAGGCC ACTTGGGCTC GAGAGGCTTT CCTGGCATCC CGGGTCCCTC AGGCCCCCCA 
GGCACCAAGG GCCTCCCAGG AGAACCGGGC CCTCAGGGAC CCCAGGGGCC AATTGGGCCT 
CCAGGAGAGA TGGGACCCAA GGGGCCGCCT GGTGCAGTGG GAGAACCGGG CCTTCCTGGG 
GAAGCCGGGA TGAAGGGTGA CCTTGGACCC CTGGGCACTC CTGGGGAGCA GGGCCTCATT 
GGGCAACGGG GAGAGCCAGG CCTTGAGGGT GACAGTGGCC CCATGGGACC TGATGGGCTG 
AAGGGGGACA GGGGAGACCC AGGGCCTGAT GGAGAACATG GCGAGAAAGG CCAGGAAGGG 
CTGATGGGTG AGGACGGGCC CCCCGGCCCC CCTGGCGTCA CTGGTGTCCG GGGTCCTGAA 
GGAAAATCAG GGAAGCAAGG CGAGAAGGGC CGCACTGGAG CCAAGGGTGC CAAGGGCTAT 
CAAGGACAGC TGGGTGAGAT GGGCGTCCCT GGAGACCCTG GACCCCCTGG CACTCCAGGC 
CCTAAAGGGT CCCGGGGCAG CCTGGGACCA ACGGGTGCTC CGGGACGCAT GGGGGCCCAA 
GGAGAACCGG GACTGGCTGG TTATGATGGA CACAAAGGCA TTGTGGGACC CCTTGGACCT 
CCTGGACCAA AAGGCGAAAA GGGGGAGCAG GGCGAGGACG GCAAGGCTGA GGGGCCCCCT 
GGGCCACCTG GAGATCGGGG CCCTGTGGGT GATCGAGGAG ACCGCGGGGA ACCGGGAGAC 
CCTGGGTACC CTGGACAGGA GGGTGTGCAA GGCCTCCGTG GAAAGCCAGG CCAGCAGGGC 
CAACCCGGGC ATCCGGGACC CCGGGGGTGG CCGGGACCCA AAGGATCGAA AGGCGCAGAG 
GGACCAAAGG GAAAGCAAGG CAAGGCAGGG GCCCCAGGCC GGAGGGGGGT CCAGGGCCTG 
CAGGGGCTGC CAGGGCCCCG GGGCGTGGTG GGGAGACAGG GCCTCGAGGG CATCGCTGGA 
CCAGATGGGC TTCCTGGCAG GGACGGGCAA GCAGGACAGC AGGGGGAGCA GGGAGACGAT 
GGGGACCCTG GCCCCATGGG CCCTGCTGGG AAGAGAGGAA ATCCAGGTGT GGCCGGCTTA 
CCTGGAGCAC AGGGACCCCC AGGATTCAAG GGTGAGAGTG GGTTACCCGG ACAGCTGGGT 
CCCCCTGGCA AGCGAGGAAC AGAGGGCAGA ACGGGGCTCC CTGGAAACCA GGGGGAGCCT 
GGGTCCAAAG GCCAGCCGGG CGACTCTGGC GAGATGGGCT TCCCAGGAAT GGCAGGTCTC 
TTCGGACCCA AGGGCCCGCC TGGAGACATT GGCTTCAAAG GCATCCAGGG CCCTCGGGGG 
CCACCTGGCT TGATGGGAAA GGAAGGCATC GTCGGGCCCC TCGGAATCCT GGGACCTTCG 
GGACTCCCGG GTCCGAAGGG TGACAAAGGC AGCCGTGGGG ACTGGGGATT GCAAGGTCCG 
AGGGGTCCTC CCGGCCCCAG AGGGCGGCCC GGCCCCCCGG GTCCTCCAGG GGGTCCTATC 
CAATTGCAAC AAGATGATCT TGGGGCAGCT TTCCAGACGT GGATGGACAC CAGTGGAGCA 
CTCAGGCCAG AGAGTTACAG CTATCCAGAC CGGCTGGTGC TGGACCAGGG AGGAGAGATC 
TTTAAAACCT TACACTACCT CAGCAACCTC ATCCAGAGCA TTAAGACGCC CCTGGGCACC 
AAAGAGAACC CCGCCCGGGT CTGCAGGGAC CTCATGGACT GTGAGCAGAA GATGGTGGAT 
GGTACCTACT GGGTGGATCC AAACCTTGGC TGCTCCTCTG ACACCATCGA GGTCTCCTGC 
AACTTCACTC ATGGTGGACA GACGTGTCTC AAGCCCATCA CGGCCTCCAA GGTCGAGTTT 
GCCATCAGCC GGGTCCAGAT GAATTTCCTG CACCTGCTAA GCTCCGAGGT GACCCAGCAC 
ATCACCATCC ACTGCCTTAA CATGACCGTG TGGCAGGAGG GCACTGGGCA GACCCCAGCC 
AAGCAGGCCG TACGCTTCCG GGCCTGGAAT GGACAGATTT TTGAAGCTGG GGGTCAGTTC 
CGGCCCGAGG TGTCCATGGA TGGCTGCAAG GTCCAAGATG GCCGCTGGCA TCAGACACTC 
TTCACCTTCC GGACCCAAGA CCCCCAACAG CTGCCCATCA TCAGTGTGGA CAACCTCCCT 
CCTGCCTCAT CAGGGAAGCA GTACCGCCTG GAAGTTGGAC CTGCGTGCTT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_011519140.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517442.1 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

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CloneID	OHu73934
Clone ID Related Accession (Same CDS sequence)	XM_011519140.1
Accession Version	XM_011519140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GTGATCGTGG CATGATGGGA 
CCCCCAGGCG TGCCTGGACC CAAGGGGTCG ATGGGTCATC CTGGAATGCC AGGTGGTATG 
GGGACCCCTG GAGAGCCTGG ACCCCAGGGT CCTCCAGGAT CTCGAGGCCC ACCAGGCATG 
AGGGGAGCAA AGGGACGTCG GGGCCCCCGA GGACCGGACG GACCAGCTGG GGAGCAAGGG 
TCCAGGGGCC TGAAGGGCCC TCCAGGACCC CAGGGCAGAC CGGGCCGGCC TGGACAGCAG 
GGTGTGGCTG GTGAGCGAGG CCACTTGGGC TCGAGAGGCT TTCCTGGCAT CCCGGGTCCC 
TCAGGCCCCC CAGGCACCAA GGGCCTCCCA GGAGAACCGG GCCCTCAGGG ACCCCAGGGG 
CCAATTGGGC CTCCAGGAGA GATGGGACCC AAGGGGCCGC CTGGTGCAGT GGGAGAACCG 
GGCCTTCCTG GGGAAGCCGG GATGAAGGGT GACCTTGGAC CCCTGGGCAC TCCTGGGGAG 
CAGGGCCTCA TTGGGCAACG GGGAGAGCCA GGCCTTGAGG GTGACAGTGG CCCCATGGGA 
CCTGATGGGC TGAAGGGGGA CAGGGGAGAC CCAGGGCCTG ATGGAGAACA TGGCGAGAAA 
GGCCAGGAAG GGCTGATGGG TGAGGACGGG CCCCCCGGCC CCCCTGGCGT CACTGGTGTC 
CGGGGTCCTG AAGGAAAATC AGGGAAGCAA GGCGAGAAGG GCCGCACTGG AGCCAAGGGT 
GCCAAGGGCT ATCAAGGACA GCTGGGTGAG ATGGGCGTCC CTGGAGACCC TGGACCCCCT 
GGCACTCCAG GCCCTAAAGG GTCCCGGGGC AGCCTGGGAC CAACGGGTGC TCCGGGACGC 
ATGGGGGCCC AAGGAGAACC GGGACTGGCT GGTTATGATG GACACAAAGG CATTGTGGGA 
CCCCTTGGAC CTCCTGGACC AAAAGGCGAA AAGGGGGAGC AGGGCGAGGA CGGCAAGGCT 
GAGGGGCCCC CTGGGCCACC TGGAGATCGG GGCCCTGTGG GTGATCGAGG AGACCGCGGG 
GAACCGGGAG ACCCTGGGTA CCCTGGACAG GAGGGTGTGC AAGGCCTCCG TGGAAAGCCA 
GGCCAGCAGG GCCAACCCGG GCATCCGGGA CCCCGGGGGT GGCCGGGACC CAAAGGATCG 
AAAGGCGCAG AGGGACCAAA GGGAAAGCAA GGCAAGGCAG GGGCCCCAGG CCGGAGGGGG 
GTCCAGGGCC TGCAGGGGCT GCCAGGGCCC CGGGGCGTGG TGGGGAGACA GGGCCTCGAG 
GGCATCGCTG GACCAGATGG GCTTCCTGGC AGGGACGGGC AAGCAGGACA GCAGGGGGAG 
CAGGGAGACG ATGGGGACCC TGGCCCCATG GGCCCTGCTG GGAAGAGAGG AAATCCAGGT 
GTGGCCGGCT TACCTGGAGC ACAGGGACCC CCAGGATTCA AGGGTGAGAG TGGGTTACCC 
GGACAGCTGG GTCCCCCTGG CAAGCGAGGA ACAGAGGGCA GAACGGGGCT CCCTGGAAAC 
CAGGGGGAGC CTGGGTCCAA AGGCCAGCCG GGCGACTCTG GCGAGATGGG CTTCCCAGGA 
ATGGCAGGTC TCTTCGGACC CAAGGGCCCG CCTGGAGACA TTGGCTTCAA AGGCATCCAG 
GGCCCTCGGG GGCCACCTGG CTTGATGGGA AAGGAAGGCA TCGTCGGGCC CCTCGGAATC 
CTGGGACCTT CGGGACTCCC GGGTCCGAAG GGTGACAAAG GCAGCCGTGG GGACTGGGGA 
TTGCAAGGTC CGAGGGGTCC TCCCGGCCCC AGAGGGCGGC CCGGCCCCCC GGGTCCTCCA 
GGGGGTCCTA TCCAATTGCA ACAAGATGAT CTTGGGGCAG CTTTCCAGAC GTGGATGGAC 
ACCAGTGGAG CACTCAGGCC AGAGAGTTAC AGCTATCCAG ACCGGCTGGT GCTGGACCAG 
GGAGGAGAGA TCTTTAAAAC CTTACACTAC CTCAGCAACC TCATCCAGAG CATTAAGACG 
CCCCTGGGCA CCAAAGAGAA CCCCGCCCGG GTCTGCAGGG ACCTCATGGA CTGTGAGCAG 
AAGATGGTGG ATGGTACCTA CTGGGTGGAT CCAAACCTTG GCTGCTCCTC TGACACCATC 
GAGGTCTCCT GCAACTTCAC TCATGGTGGA CAGACGTGTC TCAAGCCCAT CACGGCCTCC 
AAGGTCGAGT TTGCCATCAG CCGGGTCCAG ATGAATTTCC TGCACCTGCT AAGCTCCGAG 
GTGACCCAGC ACATCACCAT CCACTGCCTT AACATGACCG TGTGGCAGGA GGGCACTGGG 
CAGACCCCAG CCAAGCAGGC CGTACGCTTC CGGGCCTGGA ATGGACAGAT TTTTGAAGCT 
GGGGGTCAGT TCCGGCCCGA GGTGTCCATG GATGGCTGCA AGGTCCAAGA TGGCCGCTGG 
CATCAGACAC TCTTCACCTT CCGGACCCAA GACCCCCAAC AGCTGCCCAT CATCAGTGTG 
GACAACCTCC CTCCTGCCTC ATCAGGGAAG CAGTACCGCC TGGAAGTTGG ACCTGCGTGC 
TTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517442.1
CDS	475..6003
Translation	MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_011519140.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519140.1

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GTGATCGTGG CATGATGGGA 
CCCCCAGGCG TGCCTGGACC CAAGGGGTCG ATGGGTCATC CTGGAATGCC AGGTGGTATG 
GGGACCCCTG GAGAGCCTGG ACCCCAGGGT CCTCCAGGAT CTCGAGGCCC ACCAGGCATG 
AGGGGAGCAA AGGGACGTCG GGGCCCCCGA GGACCGGACG GACCAGCTGG GGAGCAAGGG 
TCCAGGGGCC TGAAGGGCCC TCCAGGACCC CAGGGCAGAC CGGGCCGGCC TGGACAGCAG 
GGTGTGGCTG GTGAGCGAGG CCACTTGGGC TCGAGAGGCT TTCCTGGCAT CCCGGGTCCC 
TCAGGCCCCC CAGGCACCAA GGGCCTCCCA GGAGAACCGG GCCCTCAGGG ACCCCAGGGG 
CCAATTGGGC CTCCAGGAGA GATGGGACCC AAGGGGCCGC CTGGTGCAGT GGGAGAACCG 
GGCCTTCCTG GGGAAGCCGG GATGAAGGGT GACCTTGGAC CCCTGGGCAC TCCTGGGGAG 
CAGGGCCTCA TTGGGCAACG GGGAGAGCCA GGCCTTGAGG GTGACAGTGG CCCCATGGGA 
CCTGATGGGC TGAAGGGGGA CAGGGGAGAC CCAGGGCCTG ATGGAGAACA TGGCGAGAAA 
GGCCAGGAAG GGCTGATGGG TGAGGACGGG CCCCCCGGCC CCCCTGGCGT CACTGGTGTC 
CGGGGTCCTG AAGGAAAATC AGGGAAGCAA GGCGAGAAGG GCCGCACTGG AGCCAAGGGT 
GCCAAGGGCT ATCAAGGACA GCTGGGTGAG ATGGGCGTCC CTGGAGACCC TGGACCCCCT 
GGCACTCCAG GCCCTAAAGG GTCCCGGGGC AGCCTGGGAC CAACGGGTGC TCCGGGACGC 
ATGGGGGCCC AAGGAGAACC GGGACTGGCT GGTTATGATG GACACAAAGG CATTGTGGGA 
CCCCTTGGAC CTCCTGGACC AAAAGGCGAA AAGGGGGAGC AGGGCGAGGA CGGCAAGGCT 
GAGGGGCCCC CTGGGCCACC TGGAGATCGG GGCCCTGTGG GTGATCGAGG AGACCGCGGG 
GAACCGGGAG ACCCTGGGTA CCCTGGACAG GAGGGTGTGC AAGGCCTCCG TGGAAAGCCA 
GGCCAGCAGG GCCAACCCGG GCATCCGGGA CCCCGGGGGT GGCCGGGACC CAAAGGATCG 
AAAGGCGCAG AGGGACCAAA GGGAAAGCAA GGCAAGGCAG GGGCCCCAGG CCGGAGGGGG 
GTCCAGGGCC TGCAGGGGCT GCCAGGGCCC CGGGGCGTGG TGGGGAGACA GGGCCTCGAG 
GGCATCGCTG GACCAGATGG GCTTCCTGGC AGGGACGGGC AAGCAGGACA GCAGGGGGAG 
CAGGGAGACG ATGGGGACCC TGGCCCCATG GGCCCTGCTG GGAAGAGAGG AAATCCAGGT 
GTGGCCGGCT TACCTGGAGC ACAGGGACCC CCAGGATTCA AGGGTGAGAG TGGGTTACCC 
GGACAGCTGG GTCCCCCTGG CAAGCGAGGA ACAGAGGGCA GAACGGGGCT CCCTGGAAAC 
CAGGGGGAGC CTGGGTCCAA AGGCCAGCCG GGCGACTCTG GCGAGATGGG CTTCCCAGGA 
ATGGCAGGTC TCTTCGGACC CAAGGGCCCG CCTGGAGACA TTGGCTTCAA AGGCATCCAG 
GGCCCTCGGG GGCCACCTGG CTTGATGGGA AAGGAAGGCA TCGTCGGGCC CCTCGGAATC 
CTGGGACCTT CGGGACTCCC GGGTCCGAAG GGTGACAAAG GCAGCCGTGG GGACTGGGGA 
TTGCAAGGTC CGAGGGGTCC TCCCGGCCCC AGAGGGCGGC CCGGCCCCCC GGGTCCTCCA 
GGGGGTCCTA TCCAATTGCA ACAAGATGAT CTTGGGGCAG CTTTCCAGAC GTGGATGGAC 
ACCAGTGGAG CACTCAGGCC AGAGAGTTAC AGCTATCCAG ACCGGCTGGT GCTGGACCAG 
GGAGGAGAGA TCTTTAAAAC CTTACACTAC CTCAGCAACC TCATCCAGAG CATTAAGACG 
CCCCTGGGCA CCAAAGAGAA CCCCGCCCGG GTCTGCAGGG ACCTCATGGA CTGTGAGCAG 
AAGATGGTGG ATGGTACCTA CTGGGTGGAT CCAAACCTTG GCTGCTCCTC TGACACCATC 
GAGGTCTCCT GCAACTTCAC TCATGGTGGA CAGACGTGTC TCAAGCCCAT CACGGCCTCC 
AAGGTCGAGT TTGCCATCAG CCGGGTCCAG ATGAATTTCC TGCACCTGCT AAGCTCCGAG 
GTGACCCAGC ACATCACCAT CCACTGCCTT AACATGACCG TGTGGCAGGA GGGCACTGGG 
CAGACCCCAG CCAAGCAGGC CGTACGCTTC CGGGCCTGGA ATGGACAGAT TTTTGAAGCT 
GGGGGTCAGT TCCGGCCCGA GGTGTCCATG GATGGCTGCA AGGTCCAAGA TGGCCGCTGG 
CATCAGACAC TCTTCACCTT CCGGACCCAA GACCCCCAAC AGCTGCCCAT CATCAGTGTG 
GACAACCTCC CTCCTGCCTC ATCAGGGAAG CAGTACCGCC TGGAAGTTGG ACCTGCGTGC 
TTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_011519142.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517444.1 Homo sapiens collagen type XXVII alpha 1 (COL27A1), transcript variant X7, mRNA.

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CloneID	OHu73936
Clone ID Related Accession (Same CDS sequence)	XM_011519142.2 , XM_011519142.3
Accession Version	XM_011519142.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG CCTGGAAACA CAGGCCCATG CCAGCCTCTG CCCCACCACC 
TCCCAGCTGA AGGCTGACCC CTGGAATACT GAAGATGTGG GGTCTCACCT GCCCCAGGGA 
GCGCTGGGCC CAGAGCCGTG GTTGGGGTGG AGGATCATGG ATGGGACAGG TGTGGTTGGG 
GCTGCCAGAG CGAAATGTGC CACCCCAAGC CTTCTTGGGT ATTTCCTGCC CAGGGTGGGC 
GCTCTGCATG CCTTGTCTGC TGTCTGGGGA AGGGACCATT TCTGCATCCA GGTTGGCACC 
TTCTTAGACA TGGCTGAGCC AAACCCATGT TACACATCTG GAGAAACCAG GGCTCAGAGA 
GATATGGTGC CTACTCAGAA GGGGAAACCA GGGCTCAGGA GGATAAAGAG CATGCACGAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517444.1
CDS	481..4686
Translation	MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGLETQAHASLCPTTSQLKADPWNTEDVGSHLPQGALGPEPWLGWRIMDGTGVVGAARAKCATPSLLGYFLPRVGALHALSAVWGRDHFCIQVGTFLDMAEPNPCYTSGETRAQRDMVPTQKGKPGLRRIKSMHEA

Target ORF information:

RefSeq Version	XM_011519142.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 (COL27A1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519142.2

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG CCTGGAAACA CAGGCCCATG CCAGCCTCTG CCCCACCACC 
TCCCAGCTGA AGGCTGACCC CTGGAATACT GAAGATGTGG GGTCTCACCT GCCCCAGGGA 
GCGCTGGGCC CAGAGCCGTG GTTGGGGTGG AGGATCATGG ATGGGACAGG TGTGGTTGGG 
GCTGCCAGAG CGAAATGTGC CACCCCAAGC CTTCTTGGGT ATTTCCTGCC CAGGGTGGGC 
GCTCTGCATG CCTTGTCTGC TGTCTGGGGA AGGGACCATT TCTGCATCCA GGTTGGCACC 
TTCTTAGACA TGGCTGAGCC AAACCCATGT TACACATCTG GAGAAACCAG GGCTCAGAGA 
GATATGGTGC CTACTCAGAA GGGGAAACCA GGGCTCAGGA GGATAAAGAG CATGCACGAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_011519142.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517444.1 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu73936
Clone ID Related Accession (Same CDS sequence)	XM_011519142.2 , XM_011519142.3
Accession Version	XM_011519142.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011519142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG CCTGGAAACA CAGGCCCATG CCAGCCTCTG CCCCACCACC 
TCCCAGCTGA AGGCTGACCC CTGGAATACT GAAGATGTGG GGTCTCACCT GCCCCAGGGA 
GCGCTGGGCC CAGAGCCGTG GTTGGGGTGG AGGATCATGG ATGGGACAGG TGTGGTTGGG 
GCTGCCAGAG CGAAATGTGC CACCCCAAGC CTTCTTGGGT ATTTCCTGCC CAGGGTGGGC 
GCTCTGCATG CCTTGTCTGC TGTCTGGGGA AGGGACCATT TCTGCATCCA GGTTGGCACC 
TTCTTAGACA TGGCTGAGCC AAACCCATGT TACACATCTG GAGAAACCAG GGCTCAGAGA 
GATATGGTGC CTACTCAGAA GGGGAAACCA GGGCTCAGGA GGATAAAGAG CATGCACGAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517444.1
CDS	482..4687
Translation	MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGLETQAHASLCPTTSQLKADPWNTEDVGSHLPQGALGPEPWLGWRIMDGTGVVGAARAKCATPSLLGYFLPRVGALHALSAVWGRDHFCIQVGTFLDMAEPNPCYTSGETRAQRDMVPTQKGKPGLRRIKSMHEA

Target ORF information:

RefSeq Version	XM_011519142.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519142.3

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG CCTGGAAACA CAGGCCCATG CCAGCCTCTG CCCCACCACC 
TCCCAGCTGA AGGCTGACCC CTGGAATACT GAAGATGTGG GGTCTCACCT GCCCCAGGGA 
GCGCTGGGCC CAGAGCCGTG GTTGGGGTGG AGGATCATGG ATGGGACAGG TGTGGTTGGG 
GCTGCCAGAG CGAAATGTGC CACCCCAAGC CTTCTTGGGT ATTTCCTGCC CAGGGTGGGC 
GCTCTGCATG CCTTGTCTGC TGTCTGGGGA AGGGACCATT TCTGCATCCA GGTTGGCACC 
TTCTTAGACA TGGCTGAGCC AAACCCATGT TACACATCTG GAGAAACCAG GGCTCAGAGA 
GATATGGTGC CTACTCAGAA GGGGAAACCA GGGCTCAGGA GGATAAAGAG CATGCACGAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_011519143.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517445.1 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu73937
Clone ID Related Accession (Same CDS sequence)	XM_011519143.2
Accession Version	XM_011519143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG TGAGATGGGC GTCCCTGGAG ACCCTGGACC CCCTGGCACT 
CCAGGCCCTA AAGGGTCCCG GGGCAGCCTG GGACCAACGG GTGCTCCGGG ACGCATGGGG 
GCCCAAGGAG AACCGGGACT GGCTGGTTAT GATGGACACA AAGGCATTGT GGGACCCCTT 
GGACCTCCTG GACCAAAAGG CGAAAAGCAG AGGAGAGAGC TCCGAGACAG GAAGGGAAGT 
GTTGCAGGCC ACACGGCTGG TCCTTTGCAA GCCGGGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517445.1
CDS	479..4540
Translation	MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKQRRELRDRKGSVAGHTAGPLQAGM

Target ORF information:

RefSeq Version	XM_011519143.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519143.2

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG TGAGATGGGC GTCCCTGGAG ACCCTGGACC CCCTGGCACT 
CCAGGCCCTA AAGGGTCCCG GGGCAGCCTG GGACCAACGG GTGCTCCGGG ACGCATGGGG 
GCCCAAGGAG AACCGGGACT GGCTGGTTAT GATGGACACA AAGGCATTGT GGGACCCCTT 
GGACCTCCTG GACCAAAAGG CGAAAAGCAG AGGAGAGAGC TCCGAGACAG GAAGGGAAGT 
GTTGCAGGCC ACACGGCTGG TCCTTTGCAA GCCGGGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_011519144.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517446.1 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X9, mRNA.

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CloneID	OHu73938
Clone ID Related Accession (Same CDS sequence)	XM_011519144.2
Accession Version	XM_011519144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG TGAGATGGGC GTCCCTGGAG ACCCTGGACC CCCTGGCACT 
CCAGGCCCTA AAGGGTCCCG GGGCAGCCTG GGACCAACGG GTGCTCCGGG ACGCATGGGG 
GCCCAAGGAG AACCGGGACT GGCTGGTTAT GATGGACACA AAGGCATTGT GGGACCCCTT 
GGACCTCCTG GACCAAAAGG CGAAAAGAGG AGAGAGCTCC GAGACAGGAA GGGAAGTGTT 
GCAGGCCACA CGGCTGGTCC TTTGCAAGCC GGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517446.1
CDS	479..4537
Translation	MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKRRELRDRKGSVAGHTAGPLQAGM

Target ORF information:

RefSeq Version	XM_011519144.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519144.2

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GGGTGACCTT GGACCCCTGG GCACTCCTGG GGAGCAGGGC 
CTCATTGGGC AACGGGGAGA GCCAGGCCTT GAGGGTGACA GTGGCCCCAT GGGACCTGAT 
GGGCTGAAGG GGGACAGGGG AGACCCAGGG CCTGATGGAG AACATGGCGA GAAAGGCCAG 
GAAGGGCTGA TGGGTGAGGA CGGGCCCCCC GGCCCCCCTG GCGTCACTGG TGTCCGGGGT 
CCTGAAGGAA AATCAGGGAA GCAAGGCGAG AAGGGCCGCA CTGGAGCCAA GGGTGCCAAG 
GGCTATCAAG GACAGCTGGG TGAGATGGGC GTCCCTGGAG ACCCTGGACC CCCTGGCACT 
CCAGGCCCTA AAGGGTCCCG GGGCAGCCTG GGACCAACGG GTGCTCCGGG ACGCATGGGG 
GCCCAAGGAG AACCGGGACT GGCTGGTTAT GATGGACACA AAGGCATTGT GGGACCCCTT 
GGACCTCCTG GACCAAAAGG CGAAAAGAGG AGAGAGCTCC GAGACAGGAA GGGAAGTGTT 
GCAGGCCACA CGGCTGGTCC TTTGCAAGCC GGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_011519145.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517447.1 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu73939
Clone ID Related Accession (Same CDS sequence)	XM_011519145.3 , XM_011519145.2
Accession Version	XM_011519145.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011519145.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTC CTTGGCACAG GATCTGGCAC CCAGGTGACT TAGGAGTGTT GGGTCCGATT 
GGCTACCCGG GACCCAAGGG CATGAAGGGA CTGATGGGCA GCGTGGGGGA GCCCGGACTG 
AAAGGTGATA AGGGTGAACA AGGGGTTCCA GGTGTGTCAG GAGATCCCGG ATTCCAAGGA 
GACAAGGGGA GCCAGGGGTT GCCAGGGTTC CCCGGTGCAC GGGGGAAGCC AGGGCCTCTG 
GGCAAAGTCG GAGACAAAGG ATCCATTGGG TTTCCCGGGC CCCCTGGACC CGAGGGATTC 
CCAGGAGACA TCGGCCCCCC TGGCGACAAT GGCCCAGAAG GCATGAAGGG TAAGCCTGGA 
GCCCGAGGCC TGCCGGGACC CCGTGGGCAG CTGGGGCCCG AGGGAGATGA GGGACCCATG 
GGGCCGCCAG GGGCCCCTGG CTTGGAGGGT CAGCCTGGCA GGAAGGGGTT TCCTGGGAGG 
CCCGGCCTGG ATGGCGTGAA GGGGGAACCA GGGGATCCTG GTCGGCCGGG GCCTGTGGGA 
GAGCAGGGAT TTATGGGATT CATTGGTCTG GTCGGGGAGC CAGGAATCGT GGGAGAAAAG 
GGTGATCGTG GCATGATGGG ACCCCCAGGC GTGCCTGGAC CCAAGGGGTC GATGGGTCAT 
CCTGGAATGC CAGGTGGTAT GGGGACCCCT GGAGAGCCTG GACCCCAGGG TCCTCCAGGA 
TCTCGAGGCC CACCAGGCAT GAGGGGAGCA AAGGGACGTC GGGGCCCCCG AGGACCGGAC 
GGACCAGCTG GGGAGCAAGG GTCCAGGGGC CTGAAGGGCC CTCCAGGACC CCAGGGCAGA 
CCGGGCCGGC CTGGACAGCA GGGTGTGGCT GGTGAGCGAG GCCACTTGGG CTCGAGAGGC 
TTTCCTGGCA TCCCGGGTCC CTCAGGCCCC CCAGGCACCA AGGGCCTCCC AGGAGAACCG 
GGCCCTCAGG GACCCCAGGG GCCAATTGGG CCTCCAGGAG AGATGGGACC CAAGGGGCCG 
CCTGGTGCAG TGGGAGAACC GGGCCTTCCT GGGGAAGCCG GGATGAAGGG TGACCTTGGA 
CCCCTGGGCA CTCCTGGGGA GCAGGGCCTC ATTGGGCAAC GGGGAGAGCC AGGCCTTGAG 
GGTGACAGTG GCCCCATGGG ACCTGATGGG CTGAAGGGGG ACAGGGGAGA CCCAGGGCCT 
GATGGAGAAC ATGGCGAGAA AGGCCAGGAA GGGCTGATGG GTGAGGACGG GCCCCCCGGC 
CCCCCTGGCG TCACTGGTGT CCGGGGTCCT GAAGGAAAAT CAGGGAAGCA AGGCGAGAAG 
GGCCGCACTG GAGCCAAGGG TGCCAAGGGC TATCAAGGAC AGCTGGGTGA GATGGGCGTC 
CCTGGAGACC CTGGACCCCC TGGCACTCCA GGCCCTAAAG GGTCCCGGGG CAGCCTGGGA 
CCAACGGGTG CTCCGGGACG CATGGGGGCC CAAGGAGAAC CGGGACTGGC TGGTTATGAT 
GGACACAAAG GCATTGTGGG ACCCCTTGGA CCTCCTGGAC CAAAAGGCGA AAAGGGGGAG 
CAGGGCGAGG ACGGCAAGGC TGAGGGGCCC CCTGGGCCAC CTGGAGATCG GGGCCCTGTG 
GGTGATCGAG GAGACCGCGG GGAACCGGGA GACCCTGGGT ACCCTGGACA GGAGGGTGTG 
CAAGGCCTCC GTGGAAAGCC AGGCCAGCAG GGCCAACCCG GGCATCCGGG ACCCCGGGGG 
TGGCCGGGAC CCAAAGGATC GAAAGGCGCA GAGGGACCAA AGGGAAAGCA AGGCAAGGCA 
GGGGCCCCAG GCCGGAGGGG GGTCCAGGGC CTGCAGGGGC TGCCAGGGCC CCGGGGCGTG 
GTGGGGAGAC AGGGCCTCGA GGGCATCGCT GGACCAGATG GGCTTCCTGG CAGGGACGGG 
CAAGCAGGAC AGCAGGGGGA GCAGGGAGAC GATGGGGACC CTGGCCCCAT GGGCCCTGCT 
GGGAAGAGAG GAAATCCAGG TGTGGCCGGC TTACCTGGAG CACAGGGACC CCCAGGATTC 
AAGGGTGAGA GTGGGTTACC CGGACAGCTG GGTCCCCCTG GCAAGCGAGG AACAGAGGGC 
AGAACGGGGC TCCCTGGAAA CCAGGGGGAG CCTGGGTCCA AAGGCCAGCC GGGCGACTCT 
GGCGAGATGG GCTTCCCAGG AATGGCAGGT CTCTTCGGAC CCAAGGGCCC GCCTGGAGAC 
ATTGGCTTCA AAGGCATCCA GGGCCCTCGG GGGCCACCTG GCTTGATGGG AAAGGAAGGC 
ATCGTCGGGC CCCTCGGAAT CCTGGGACCT TCGGGACTCC CGGGTCCGAA GGGTGACAAA 
GGCAGCCGTG GGGACTGGGG ATTGCAAGGT CCGAGGGGTC CTCCCGGCCC CAGAGGGCGG 
CCCGGCCCCC CGGGTCCTCC AGGGGGTCCT ATCCAATTGC AACAAGATGA TCTTGGGGCA 
GCTTTCCAGA CGTGGATGGA CACCAGTGGA GCACTCAGGC CAGAGAGTTA CAGCTATCCA 
GACCGGCTGG TGCTGGACCA GGGAGGAGAG ATCTTTAAAA CCTTACACTA CCTCAGCAAC 
CTCATCCAGA GCATTAAGAC GCCCCTGGGC ACCAAAGAGA ACCCCGCCCG GGTCTGCAGG 
GACCTCATGG ACTGTGAGCA GAAGATGGTG GATGGTACCT ACTGGGTGGA TCCAAACCTT 
GGCTGCTCCT CTGACACCAT CGAGGTCTCC TGCAACTTCA CTCATGGTGG ACAGACGTGT 
CTCAAGCCCA TCACGGCCTC CAAGGTCGAG TTTGCCATCA GCCGGGTCCA GATGAATTTC 
CTGCACCTGC TAAGCTCCGA GGTGACCCAG CACATCACCA TCCACTGCCT TAACATGACC 
GTGTGGCAGG AGGGCACTGG GCAGACCCCA GCCAAGCAGG CCGTACGCTT CCGGGCCTGG 
AATGGACAGA TTTTTGAAGC TGGGGGTCAG TTCCGGCCCG AGGTGTCCAT GGATGGCTGC 
AAGGTCCAAG ATGGCCGCTG GCATCAGACA CTCTTCACCT TCCGGACCCA AGACCCCCAA 
CAGCTGCCCA TCATCAGTGT GGACAACCTC CCTCCTGCCT CATCAGGGAA GCAGTACCGC 
CTGGAAGTTG GACCTGCGTG CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517447.1
CDS	83..3232
Translation	MCSPWHRIWHPGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_011519145.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519145.3

ORF Insert Sequence:

ATGTGTAGTC CTTGGCACAG GATCTGGCAC CCAGGTGACT TAGGAGTGTT GGGTCCGATT 
GGCTACCCGG GACCCAAGGG CATGAAGGGA CTGATGGGCA GCGTGGGGGA GCCCGGACTG 
AAAGGTGATA AGGGTGAACA AGGGGTTCCA GGTGTGTCAG GAGATCCCGG ATTCCAAGGA 
GACAAGGGGA GCCAGGGGTT GCCAGGGTTC CCCGGTGCAC GGGGGAAGCC AGGGCCTCTG 
GGCAAAGTCG GAGACAAAGG ATCCATTGGG TTTCCCGGGC CCCCTGGACC CGAGGGATTC 
CCAGGAGACA TCGGCCCCCC TGGCGACAAT GGCCCAGAAG GCATGAAGGG TAAGCCTGGA 
GCCCGAGGCC TGCCGGGACC CCGTGGGCAG CTGGGGCCCG AGGGAGATGA GGGACCCATG 
GGGCCGCCAG GGGCCCCTGG CTTGGAGGGT CAGCCTGGCA GGAAGGGGTT TCCTGGGAGG 
CCCGGCCTGG ATGGCGTGAA GGGGGAACCA GGGGATCCTG GTCGGCCGGG GCCTGTGGGA 
GAGCAGGGAT TTATGGGATT CATTGGTCTG GTCGGGGAGC CAGGAATCGT GGGAGAAAAG 
GGTGATCGTG GCATGATGGG ACCCCCAGGC GTGCCTGGAC CCAAGGGGTC GATGGGTCAT 
CCTGGAATGC CAGGTGGTAT GGGGACCCCT GGAGAGCCTG GACCCCAGGG TCCTCCAGGA 
TCTCGAGGCC CACCAGGCAT GAGGGGAGCA AAGGGACGTC GGGGCCCCCG AGGACCGGAC 
GGACCAGCTG GGGAGCAAGG GTCCAGGGGC CTGAAGGGCC CTCCAGGACC CCAGGGCAGA 
CCGGGCCGGC CTGGACAGCA GGGTGTGGCT GGTGAGCGAG GCCACTTGGG CTCGAGAGGC 
TTTCCTGGCA TCCCGGGTCC CTCAGGCCCC CCAGGCACCA AGGGCCTCCC AGGAGAACCG 
GGCCCTCAGG GACCCCAGGG GCCAATTGGG CCTCCAGGAG AGATGGGACC CAAGGGGCCG 
CCTGGTGCAG TGGGAGAACC GGGCCTTCCT GGGGAAGCCG GGATGAAGGG TGACCTTGGA 
CCCCTGGGCA CTCCTGGGGA GCAGGGCCTC ATTGGGCAAC GGGGAGAGCC AGGCCTTGAG 
GGTGACAGTG GCCCCATGGG ACCTGATGGG CTGAAGGGGG ACAGGGGAGA CCCAGGGCCT 
GATGGAGAAC ATGGCGAGAA AGGCCAGGAA GGGCTGATGG GTGAGGACGG GCCCCCCGGC 
CCCCCTGGCG TCACTGGTGT CCGGGGTCCT GAAGGAAAAT CAGGGAAGCA AGGCGAGAAG 
GGCCGCACTG GAGCCAAGGG TGCCAAGGGC TATCAAGGAC AGCTGGGTGA GATGGGCGTC 
CCTGGAGACC CTGGACCCCC TGGCACTCCA GGCCCTAAAG GGTCCCGGGG CAGCCTGGGA 
CCAACGGGTG CTCCGGGACG CATGGGGGCC CAAGGAGAAC CGGGACTGGC TGGTTATGAT 
GGACACAAAG GCATTGTGGG ACCCCTTGGA CCTCCTGGAC CAAAAGGCGA AAAGGGGGAG 
CAGGGCGAGG ACGGCAAGGC TGAGGGGCCC CCTGGGCCAC CTGGAGATCG GGGCCCTGTG 
GGTGATCGAG GAGACCGCGG GGAACCGGGA GACCCTGGGT ACCCTGGACA GGAGGGTGTG 
CAAGGCCTCC GTGGAAAGCC AGGCCAGCAG GGCCAACCCG GGCATCCGGG ACCCCGGGGG 
TGGCCGGGAC CCAAAGGATC GAAAGGCGCA GAGGGACCAA AGGGAAAGCA AGGCAAGGCA 
GGGGCCCCAG GCCGGAGGGG GGTCCAGGGC CTGCAGGGGC TGCCAGGGCC CCGGGGCGTG 
GTGGGGAGAC AGGGCCTCGA GGGCATCGCT GGACCAGATG GGCTTCCTGG CAGGGACGGG 
CAAGCAGGAC AGCAGGGGGA GCAGGGAGAC GATGGGGACC CTGGCCCCAT GGGCCCTGCT 
GGGAAGAGAG GAAATCCAGG TGTGGCCGGC TTACCTGGAG CACAGGGACC CCCAGGATTC 
AAGGGTGAGA GTGGGTTACC CGGACAGCTG GGTCCCCCTG GCAAGCGAGG AACAGAGGGC 
AGAACGGGGC TCCCTGGAAA CCAGGGGGAG CCTGGGTCCA AAGGCCAGCC GGGCGACTCT 
GGCGAGATGG GCTTCCCAGG AATGGCAGGT CTCTTCGGAC CCAAGGGCCC GCCTGGAGAC 
ATTGGCTTCA AAGGCATCCA GGGCCCTCGG GGGCCACCTG GCTTGATGGG AAAGGAAGGC 
ATCGTCGGGC CCCTCGGAAT CCTGGGACCT TCGGGACTCC CGGGTCCGAA GGGTGACAAA 
GGCAGCCGTG GGGACTGGGG ATTGCAAGGT CCGAGGGGTC CTCCCGGCCC CAGAGGGCGG 
CCCGGCCCCC CGGGTCCTCC AGGGGGTCCT ATCCAATTGC AACAAGATGA TCTTGGGGCA 
GCTTTCCAGA CGTGGATGGA CACCAGTGGA GCACTCAGGC CAGAGAGTTA CAGCTATCCA 
GACCGGCTGG TGCTGGACCA GGGAGGAGAG ATCTTTAAAA CCTTACACTA CCTCAGCAAC 
CTCATCCAGA GCATTAAGAC GCCCCTGGGC ACCAAAGAGA ACCCCGCCCG GGTCTGCAGG 
GACCTCATGG ACTGTGAGCA GAAGATGGTG GATGGTACCT ACTGGGTGGA TCCAAACCTT 
GGCTGCTCCT CTGACACCAT CGAGGTCTCC TGCAACTTCA CTCATGGTGG ACAGACGTGT 
CTCAAGCCCA TCACGGCCTC CAAGGTCGAG TTTGCCATCA GCCGGGTCCA GATGAATTTC 
CTGCACCTGC TAAGCTCCGA GGTGACCCAG CACATCACCA TCCACTGCCT TAACATGACC 
GTGTGGCAGG AGGGCACTGG GCAGACCCCA GCCAAGCAGG CCGTACGCTT CCGGGCCTGG 
AATGGACAGA TTTTTGAAGC TGGGGGTCAG TTCCGGCCCG AGGTGTCCAT GGATGGCTGC 
AAGGTCCAAG ATGGCCGCTG GCATCAGACA CTCTTCACCT TCCGGACCCA AGACCCCCAA 
CAGCTGCCCA TCATCAGTGT GGACAACCTC CCTCCTGCCT CATCAGGGAA GCAGTACCGC 
CTGGAAGTTG GACCTGCGTG CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_011519145.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517447.1 Homo sapiens collagen type XXVII alpha 1 (COL27A1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu73939
Clone ID Related Accession (Same CDS sequence)	XM_011519145.3 , XM_011519145.2
Accession Version	XM_011519145.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTC CTTGGCACAG GATCTGGCAC CCAGGTGACT TAGGAGTGTT GGGTCCGATT 
GGCTACCCGG GACCCAAGGG CATGAAGGGA CTGATGGGCA GCGTGGGGGA GCCCGGACTG 
AAAGGTGATA AGGGTGAACA AGGGGTTCCA GGTGTGTCAG GAGATCCCGG ATTCCAAGGA 
GACAAGGGGA GCCAGGGGTT GCCAGGGTTC CCCGGTGCAC GGGGGAAGCC AGGGCCTCTG 
GGCAAAGTCG GAGACAAAGG ATCCATTGGG TTTCCCGGGC CCCCTGGACC CGAGGGATTC 
CCAGGAGACA TCGGCCCCCC TGGCGACAAT GGCCCAGAAG GCATGAAGGG TAAGCCTGGA 
GCCCGAGGCC TGCCGGGACC CCGTGGGCAG CTGGGGCCCG AGGGAGATGA GGGACCCATG 
GGGCCGCCAG GGGCCCCTGG CTTGGAGGGT CAGCCTGGCA GGAAGGGGTT TCCTGGGAGG 
CCCGGCCTGG ATGGCGTGAA GGGGGAACCA GGGGATCCTG GTCGGCCGGG GCCTGTGGGA 
GAGCAGGGAT TTATGGGATT CATTGGTCTG GTCGGGGAGC CAGGAATCGT GGGAGAAAAG 
GGTGATCGTG GCATGATGGG ACCCCCAGGC GTGCCTGGAC CCAAGGGGTC GATGGGTCAT 
CCTGGAATGC CAGGTGGTAT GGGGACCCCT GGAGAGCCTG GACCCCAGGG TCCTCCAGGA 
TCTCGAGGCC CACCAGGCAT GAGGGGAGCA AAGGGACGTC GGGGCCCCCG AGGACCGGAC 
GGACCAGCTG GGGAGCAAGG GTCCAGGGGC CTGAAGGGCC CTCCAGGACC CCAGGGCAGA 
CCGGGCCGGC CTGGACAGCA GGGTGTGGCT GGTGAGCGAG GCCACTTGGG CTCGAGAGGC 
TTTCCTGGCA TCCCGGGTCC CTCAGGCCCC CCAGGCACCA AGGGCCTCCC AGGAGAACCG 
GGCCCTCAGG GACCCCAGGG GCCAATTGGG CCTCCAGGAG AGATGGGACC CAAGGGGCCG 
CCTGGTGCAG TGGGAGAACC GGGCCTTCCT GGGGAAGCCG GGATGAAGGG TGACCTTGGA 
CCCCTGGGCA CTCCTGGGGA GCAGGGCCTC ATTGGGCAAC GGGGAGAGCC AGGCCTTGAG 
GGTGACAGTG GCCCCATGGG ACCTGATGGG CTGAAGGGGG ACAGGGGAGA CCCAGGGCCT 
GATGGAGAAC ATGGCGAGAA AGGCCAGGAA GGGCTGATGG GTGAGGACGG GCCCCCCGGC 
CCCCCTGGCG TCACTGGTGT CCGGGGTCCT GAAGGAAAAT CAGGGAAGCA AGGCGAGAAG 
GGCCGCACTG GAGCCAAGGG TGCCAAGGGC TATCAAGGAC AGCTGGGTGA GATGGGCGTC 
CCTGGAGACC CTGGACCCCC TGGCACTCCA GGCCCTAAAG GGTCCCGGGG CAGCCTGGGA 
CCAACGGGTG CTCCGGGACG CATGGGGGCC CAAGGAGAAC CGGGACTGGC TGGTTATGAT 
GGACACAAAG GCATTGTGGG ACCCCTTGGA CCTCCTGGAC CAAAAGGCGA AAAGGGGGAG 
CAGGGCGAGG ACGGCAAGGC TGAGGGGCCC CCTGGGCCAC CTGGAGATCG GGGCCCTGTG 
GGTGATCGAG GAGACCGCGG GGAACCGGGA GACCCTGGGT ACCCTGGACA GGAGGGTGTG 
CAAGGCCTCC GTGGAAAGCC AGGCCAGCAG GGCCAACCCG GGCATCCGGG ACCCCGGGGG 
TGGCCGGGAC CCAAAGGATC GAAAGGCGCA GAGGGACCAA AGGGAAAGCA AGGCAAGGCA 
GGGGCCCCAG GCCGGAGGGG GGTCCAGGGC CTGCAGGGGC TGCCAGGGCC CCGGGGCGTG 
GTGGGGAGAC AGGGCCTCGA GGGCATCGCT GGACCAGATG GGCTTCCTGG CAGGGACGGG 
CAAGCAGGAC AGCAGGGGGA GCAGGGAGAC GATGGGGACC CTGGCCCCAT GGGCCCTGCT 
GGGAAGAGAG GAAATCCAGG TGTGGCCGGC TTACCTGGAG CACAGGGACC CCCAGGATTC 
AAGGGTGAGA GTGGGTTACC CGGACAGCTG GGTCCCCCTG GCAAGCGAGG AACAGAGGGC 
AGAACGGGGC TCCCTGGAAA CCAGGGGGAG CCTGGGTCCA AAGGCCAGCC GGGCGACTCT 
GGCGAGATGG GCTTCCCAGG AATGGCAGGT CTCTTCGGAC CCAAGGGCCC GCCTGGAGAC 
ATTGGCTTCA AAGGCATCCA GGGCCCTCGG GGGCCACCTG GCTTGATGGG AAAGGAAGGC 
ATCGTCGGGC CCCTCGGAAT CCTGGGACCT TCGGGACTCC CGGGTCCGAA GGGTGACAAA 
GGCAGCCGTG GGGACTGGGG ATTGCAAGGT CCGAGGGGTC CTCCCGGCCC CAGAGGGCGG 
CCCGGCCCCC CGGGTCCTCC AGGGGGTCCT ATCCAATTGC AACAAGATGA TCTTGGGGCA 
GCTTTCCAGA CGTGGATGGA CACCAGTGGA GCACTCAGGC CAGAGAGTTA CAGCTATCCA 
GACCGGCTGG TGCTGGACCA GGGAGGAGAG ATCTTTAAAA CCTTACACTA CCTCAGCAAC 
CTCATCCAGA GCATTAAGAC GCCCCTGGGC ACCAAAGAGA ACCCCGCCCG GGTCTGCAGG 
GACCTCATGG ACTGTGAGCA GAAGATGGTG GATGGTACCT ACTGGGTGGA TCCAAACCTT 
GGCTGCTCCT CTGACACCAT CGAGGTCTCC TGCAACTTCA CTCATGGTGG ACAGACGTGT 
CTCAAGCCCA TCACGGCCTC CAAGGTCGAG TTTGCCATCA GCCGGGTCCA GATGAATTTC 
CTGCACCTGC TAAGCTCCGA GGTGACCCAG CACATCACCA TCCACTGCCT TAACATGACC 
GTGTGGCAGG AGGGCACTGG GCAGACCCCA GCCAAGCAGG CCGTACGCTT CCGGGCCTGG 
AATGGACAGA TTTTTGAAGC TGGGGGTCAG TTCCGGCCCG AGGTGTCCAT GGATGGCTGC 
AAGGTCCAAG ATGGCCGCTG GCATCAGACA CTCTTCACCT TCCGGACCCA AGACCCCCAA 
CAGCTGCCCA TCATCAGTGT GGACAACCTC CCTCCTGCCT CATCAGGGAA GCAGTACCGC 
CTGGAAGTTG GACCTGCGTG CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517447.1
CDS	82..3231
Translation	MCSPWHRIWHPGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_011519145.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 (COL27A1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519145.2

ORF Insert Sequence:

ATGTGTAGTC CTTGGCACAG GATCTGGCAC CCAGGTGACT TAGGAGTGTT GGGTCCGATT 
GGCTACCCGG GACCCAAGGG CATGAAGGGA CTGATGGGCA GCGTGGGGGA GCCCGGACTG 
AAAGGTGATA AGGGTGAACA AGGGGTTCCA GGTGTGTCAG GAGATCCCGG ATTCCAAGGA 
GACAAGGGGA GCCAGGGGTT GCCAGGGTTC CCCGGTGCAC GGGGGAAGCC AGGGCCTCTG 
GGCAAAGTCG GAGACAAAGG ATCCATTGGG TTTCCCGGGC CCCCTGGACC CGAGGGATTC 
CCAGGAGACA TCGGCCCCCC TGGCGACAAT GGCCCAGAAG GCATGAAGGG TAAGCCTGGA 
GCCCGAGGCC TGCCGGGACC CCGTGGGCAG CTGGGGCCCG AGGGAGATGA GGGACCCATG 
GGGCCGCCAG GGGCCCCTGG CTTGGAGGGT CAGCCTGGCA GGAAGGGGTT TCCTGGGAGG 
CCCGGCCTGG ATGGCGTGAA GGGGGAACCA GGGGATCCTG GTCGGCCGGG GCCTGTGGGA 
GAGCAGGGAT TTATGGGATT CATTGGTCTG GTCGGGGAGC CAGGAATCGT GGGAGAAAAG 
GGTGATCGTG GCATGATGGG ACCCCCAGGC GTGCCTGGAC CCAAGGGGTC GATGGGTCAT 
CCTGGAATGC CAGGTGGTAT GGGGACCCCT GGAGAGCCTG GACCCCAGGG TCCTCCAGGA 
TCTCGAGGCC CACCAGGCAT GAGGGGAGCA AAGGGACGTC GGGGCCCCCG AGGACCGGAC 
GGACCAGCTG GGGAGCAAGG GTCCAGGGGC CTGAAGGGCC CTCCAGGACC CCAGGGCAGA 
CCGGGCCGGC CTGGACAGCA GGGTGTGGCT GGTGAGCGAG GCCACTTGGG CTCGAGAGGC 
TTTCCTGGCA TCCCGGGTCC CTCAGGCCCC CCAGGCACCA AGGGCCTCCC AGGAGAACCG 
GGCCCTCAGG GACCCCAGGG GCCAATTGGG CCTCCAGGAG AGATGGGACC CAAGGGGCCG 
CCTGGTGCAG TGGGAGAACC GGGCCTTCCT GGGGAAGCCG GGATGAAGGG TGACCTTGGA 
CCCCTGGGCA CTCCTGGGGA GCAGGGCCTC ATTGGGCAAC GGGGAGAGCC AGGCCTTGAG 
GGTGACAGTG GCCCCATGGG ACCTGATGGG CTGAAGGGGG ACAGGGGAGA CCCAGGGCCT 
GATGGAGAAC ATGGCGAGAA AGGCCAGGAA GGGCTGATGG GTGAGGACGG GCCCCCCGGC 
CCCCCTGGCG TCACTGGTGT CCGGGGTCCT GAAGGAAAAT CAGGGAAGCA AGGCGAGAAG 
GGCCGCACTG GAGCCAAGGG TGCCAAGGGC TATCAAGGAC AGCTGGGTGA GATGGGCGTC 
CCTGGAGACC CTGGACCCCC TGGCACTCCA GGCCCTAAAG GGTCCCGGGG CAGCCTGGGA 
CCAACGGGTG CTCCGGGACG CATGGGGGCC CAAGGAGAAC CGGGACTGGC TGGTTATGAT 
GGACACAAAG GCATTGTGGG ACCCCTTGGA CCTCCTGGAC CAAAAGGCGA AAAGGGGGAG 
CAGGGCGAGG ACGGCAAGGC TGAGGGGCCC CCTGGGCCAC CTGGAGATCG GGGCCCTGTG 
GGTGATCGAG GAGACCGCGG GGAACCGGGA GACCCTGGGT ACCCTGGACA GGAGGGTGTG 
CAAGGCCTCC GTGGAAAGCC AGGCCAGCAG GGCCAACCCG GGCATCCGGG ACCCCGGGGG 
TGGCCGGGAC CCAAAGGATC GAAAGGCGCA GAGGGACCAA AGGGAAAGCA AGGCAAGGCA 
GGGGCCCCAG GCCGGAGGGG GGTCCAGGGC CTGCAGGGGC TGCCAGGGCC CCGGGGCGTG 
GTGGGGAGAC AGGGCCTCGA GGGCATCGCT GGACCAGATG GGCTTCCTGG CAGGGACGGG 
CAAGCAGGAC AGCAGGGGGA GCAGGGAGAC GATGGGGACC CTGGCCCCAT GGGCCCTGCT 
GGGAAGAGAG GAAATCCAGG TGTGGCCGGC TTACCTGGAG CACAGGGACC CCCAGGATTC 
AAGGGTGAGA GTGGGTTACC CGGACAGCTG GGTCCCCCTG GCAAGCGAGG AACAGAGGGC 
AGAACGGGGC TCCCTGGAAA CCAGGGGGAG CCTGGGTCCA AAGGCCAGCC GGGCGACTCT 
GGCGAGATGG GCTTCCCAGG AATGGCAGGT CTCTTCGGAC CCAAGGGCCC GCCTGGAGAC 
ATTGGCTTCA AAGGCATCCA GGGCCCTCGG GGGCCACCTG GCTTGATGGG AAAGGAAGGC 
ATCGTCGGGC CCCTCGGAAT CCTGGGACCT TCGGGACTCC CGGGTCCGAA GGGTGACAAA 
GGCAGCCGTG GGGACTGGGG ATTGCAAGGT CCGAGGGGTC CTCCCGGCCC CAGAGGGCGG 
CCCGGCCCCC CGGGTCCTCC AGGGGGTCCT ATCCAATTGC AACAAGATGA TCTTGGGGCA 
GCTTTCCAGA CGTGGATGGA CACCAGTGGA GCACTCAGGC CAGAGAGTTA CAGCTATCCA 
GACCGGCTGG TGCTGGACCA GGGAGGAGAG ATCTTTAAAA CCTTACACTA CCTCAGCAAC 
CTCATCCAGA GCATTAAGAC GCCCCTGGGC ACCAAAGAGA ACCCCGCCCG GGTCTGCAGG 
GACCTCATGG ACTGTGAGCA GAAGATGGTG GATGGTACCT ACTGGGTGGA TCCAAACCTT 
GGCTGCTCCT CTGACACCAT CGAGGTCTCC TGCAACTTCA CTCATGGTGG ACAGACGTGT 
CTCAAGCCCA TCACGGCCTC CAAGGTCGAG TTTGCCATCA GCCGGGTCCA GATGAATTTC 
CTGCACCTGC TAAGCTCCGA GGTGACCCAG CACATCACCA TCCACTGCCT TAACATGACC 
GTGTGGCAGG AGGGCACTGG GCAGACCCCA GCCAAGCAGG CCGTACGCTT CCGGGCCTGG 
AATGGACAGA TTTTTGAAGC TGGGGGTCAG TTCCGGCCCG AGGTGTCCAT GGATGGCTGC 
AAGGTCCAAG ATGGCCGCTG GCATCAGACA CTCTTCACCT TCCGGACCCA AGACCCCCAA 
CAGCTGCCCA TCATCAGTGT GGACAACCTC CCTCCTGCCT CATCAGGGAA GCAGTACCGC 
CTGGAAGTTG GACCTGCGTG CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_017015239.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870728.1 Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X10, mRNA.

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CloneID	OHu98967
Clone ID Related Accession (Same CDS sequence)	XM_017015239.1
Accession Version	XM_017015239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXVII) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GAAGTCCGTG GACAAGGCTT ACTTGGAACA GGAGCTCTGG 
GTGACCTTGG ACCCCTGGGC ACTCCTGGGG AGCAGGGCCT CATTGGGCAA CGGGGAGAGC 
CAGGCCTTGA GGGTGACAGT GGCCCCATGG GACCTGATGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870728.1
CDS	480..4124
Translation	MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSRPAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHKPPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSEASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSSSPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAGMKKSVDKAYLEQELWVTLDPWALLGSRASLGNGESQALRVTVAPWDLMG

Target ORF information:

RefSeq Version	XM_017015239.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015239.1

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGG CGGCGGCGGC GGCGCGCGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CATCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCGGGCT TCATCTTTAC GCAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CACCGTCATT CCTGCCGCCT TGGGCACAGA GCTGGCACTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACGCAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTCGGG 
TCCCGGCGCT CAGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACTGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCTG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTCT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG ACCTCTCTTC TCCCAAGACT CTGGCAGACC TTTTACCTTC 
CAGTCCGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC AGCCCTGGGG 
TCACTGCCAG CAGGCAGGGG ACCCAGGGGG ACTGTGGCAC CCGCCACGCC CACCAAGCCC 
CAAAGGACTA GCCCCACAAA CCCTCACCAG CATATGGCGG TGGGAGGCCC AGCCCAAACC 
CCGCTGCTAC CTGCCAAGCT GTCAGCCAGT AACGCACTTG ATCCCATGCT CCCAGCCTCT 
GTTGGCGGCT CTACCAGAAC GCCTCGCCCT GCGGCCGCTC AACCATCACA GAAGATCACA 
GCCACCAAAA TCCCCAAAAG CCTCCCTACC AAGCCTTCGG CCCCTTCTAC TTCAATTGTG 
CCCATCAAAA GCCCCCATCC TACCCAGAAA ACAGCTCCAT CTTCATTTAC AAAGTCAGCC 
CTACCCACTC AGAAGCAAGT GCCACCTACT TCCCGTCCAG TTCCTGCCAG AGTCTCCCGT 
CCCGCAGAGA AGCCCATCCA GAGGAACCCG GGAATGCCCA GGCCCCCACC GCCCAGCACC 
CGGCCCCTAC CTCCTACCAC CAGCTCCTCT AAAAAACCCA TTCCCACACT AGCTCGGACT 
GAGGCCAAGA TAACCAGCCA TGCCAGTAAG CCGGCCTCTG CCCGCACCAG CACCCACAAA 
CCTCCCCCAT TTACTGCTTT ATCCTCATCT CCTGCCCCTA CTCCTGGTTC TACCAGGAGT 
ACTCGGCCAC CAGCCACGAT GGTACCTCCA ACTTCGGGCA CCAGCACTCC CAGAACAGCA 
CCTGCCGTCC CCACTCCTGG CTCAGCTCCC ACTGGAAGCA AGAAGCCCAT TGGATCGGAA 
GCCTCAAAGA AAGCCGGACC CAAGAGCAGC CCCCGGAAGC CTGTCCCCCT CAGACCTGGG 
AAGGCAGCCA GGGATGTCCC CTTGAGCGAT CTGACAACCA GGCCTAGCCC CAGACAGCCC 
CAGCCCAGTC AGCAGACCAC CCCGGCCCTG GTATTGGCCC CGGCGCAATT CCTGTCCTCC 
AGCCCCCGGC CCACGAGCAG TGGCTATTCG ATCTTCCACC TGGCAGGATC TACGCCTTTC 
CCTCTGCTGA TGGGGCCTCC GGGACCCAAG GGAGACTGTG GCTTGCCGGG TCCCCCTGGG 
CTACCTGGGC TACCTGGAAT CCCTGGTGCA CGTGGGCCTC GGGGTCCTCC TGGGCCTTAT 
GGAAATCCAG GTCTCCCCGG CCCTCCTGGA GCCAAAGGAC AGAAAGGGGA CCCAGGGCTC 
TCACCAGGAA AGGCCCACGA TGGGGCAAAG GGTGACATGG GCTTGCCTGG GCTCTCCGGG 
AATCCAGGAC CTCCGGGACG AAAGGGACAC AAGGGCTATC CTGGACCGGC AGGGCACCCC 
GGAGAACAGG GGCAGCCAGG ACCTGAGGGC AGCCCAGGGG CCAAAGGTTA CCCTGGCAGG 
CAGGGGTTAC CTGGACCGGT AGGAGATCCC GGCCCCAAAG GCAGCAGGGG CTACATTGGG 
CTCCCAGGGC TCTTCGGCCT GCCAGGGTCT GATGGAGAAC GAGGCCTGCC TGGCGTTCCT 
GGCAAGAGGG GCAAGATGGG TATGCCGGGG TTTCCTGGAG TCTTTGGGGA AAGAGGCCCT 
CCTGGACTGG ATGGAAATCC TGGAGAACTG GGCCTGCCAG GCCCCCCTGG AGTCCCCGGC 
CTCATTGGTG ACTTAGGAGT GTTGGGTCCG ATTGGCTACC CGGGACCCAA GGGCATGAAG 
GGACTGATGG GCAGCGTGGG GGAGCCCGGA CTGAAAGGTG ATAAGGGTGA ACAAGGGGTT 
CCAGGTGTGT CAGGAGATCC CGGATTCCAA GGAGACAAGG GGAGCCAGGG GTTGCCAGGG 
TTCCCCGGTG CACGGGGGAA GCCAGGGCCT CTGGGCAAAG TCGGAGACAA AGGATCCATT 
GGGTTTCCCG GGCCCCCTGG ACCCGAGGGA TTCCCAGGAG ACATCGGCCC CCCTGGCGAC 
AATGGCCCAG AAGGCATGAA GGGTAAGCCT GGAGCCCGAG GCCTGCCGGG ACCCCGTGGG 
CAGCTGGGGC CCGAGGGAGA TGAGGGACCC ATGGGGCCGC CAGGGGCCCC TGGCTTGGAG 
GGTCAGCCTG GCAGGAAGGG GTTTCCTGGG AGGCCCGGCC TGGATGGCGT GAAGGGGGAA 
CCAGGGGATC CTGGTCGGCC GGGGCCTGTG GGAGAGCAGG GATTTATGGG ATTCATTGGT 
CTGGTCGGGG AGCCAGGAAT CGTGGGAGAA AAGGGTGATC GTGGCATGAT GGGACCCCCA 
GGCGTGCCTG GACCCAAGGG GTCGATGGGT CATCCTGGAA TGCCAGGTGG TATGGGGACC 
CCTGGAGAGC CTGGACCCCA GGGTCCTCCA GGATCTCGAG GCCCACCAGG CATGAGGGGA 
GCAAAGGGAC GTCGGGGCCC CCGAGGACCG GACGGACCAG CTGGGGAGCA AGGGTCCAGG 
GGCCTGAAGG GCCCTCCAGG ACCCCAGGGC AGACCGGGCC GGCCTGGACA GCAGGGTGTG 
GCTGGTGAGC GAGGCCACTT GGGCTCGAGA GGCTTTCCTG GCATCCCGGG TCCCTCAGGC 
CCCCCAGGCA CCAAGGGCCT CCCAGGAGAA CCGGGCCCTC AGGGACCCCA GGGGCCAATT 
GGGCCTCCAG GAGAGATGGG ACCCAAGGGG CCGCCTGGTG CAGTGGGAGA ACCGGGCCTT 
CCTGGGGAAG CCGGGATGAA GAAGTCCGTG GACAAGGCTT ACTTGGAACA GGAGCTCTGG 
GTGACCTTGG ACCCCTGGGC ACTCCTGGGG AGCAGGGCCT CATTGGGCAA CGGGGAGAGC 
CAGGCCTTGA GGGTGACAGT GGCCCCATGG GACCTGATGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL27A1
Entrez Gene ID	85301
Full Name	collagen type XXVII alpha 1 chain
Synonyms	STLS
General protein information	Preferred Names collagen type XXVII alpha 1 chain Names collagen alpha-1(XXVII) chain collagen, type XXVII, alpha 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q32
Summary	This gene encodes a member of the fibrillar collagen family, and plays a role during the calcification of cartilage and the transition of cartilage to bone. The encoded protein product is a preproprotein. It includes an N-terminal signal peptide, which is followed by an N-terminal propetide, mature peptide and a C-terminal propeptide. The N-terminal propeptide contains thrombospondin N-terminal-like and laminin G-like domains. The mature peptide is a major triple-helical region. The C-terminal propeptide, also known as COLFI domain, plays crucial roles in tissue growth and repair. Mutations in this gene cause Steel syndrome. Alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Sep 2014].
Disorder MIM:	608461
Disorder Html:	?Steel syndrome, 615155 (3)

Bos taurus (cattle)	COL27A1	NP_001193609.1
Rattus norvegicus (Norway rat)	Col27a1	NP_942042.1
Homo sapiens (human)	COL27A1	NP_116277.2
Canis lupus familiaris (dog)	COL27A1	XP_005627059.1
Pan troglodytes (chimpanzee)	COL27A1	XP_520208.3
Gallus gallus (chicken)	COL27A1	XP_415514.3
Mus musculus (house mouse)	Col27a1	NP_079961.3
Xenopus tropicalis (tropical clawed frog)	LOC100490770	XP_004916755.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL27A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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