H2BC12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	H2BC12
Entrez Gene ID	85236
Full Name	histone cluster 1 H2B family member k
Synonyms	H2B/S,H2BFAiii,H2BFT,H2BK
General protein information	Preferred Names histone cluster 1 H2B family member k Names histone H2B type 1-K H2B K H2B histone family, member T HIRA-interacting protein 1 histone 1, H2bk histone cluster 1, H2bk histone family member
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p22.1
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family. The protein encoded is an antimicrobial protein with antibacterial and antifungal activity. Two transcripts that encode the same protein have been identified for this gene, which is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015].
Disorder MIM:	615045

mRNA and Protein(s)

mRNA	Protein	Name
NM_001312653.1	NP_001299582.1	histone H2B type 1-K
NM_001312653.2	NP_001299582.1	histone H2B type 1-K
NM_080593.2	NP_542160.1	histone H2B type 1-K

Pathways from BioSystems

Homologies

Homo sapiens (human)	HIST1H2BK	NP_542160.1
Mus musculus (house mouse)	Hist1h2bk	NP_783596.1
Canis lupus familiaris (dog)	LOC488303	XP_859540.1
	HIST1H2BK	XP_001092808.1
Pan troglodytes (chimpanzee)	HIST1H2BK	XP_003311159.1
Rattus norvegicus (Norway rat)	Hist1h2bk	NP_001102870.1
Bos taurus (cattle)	HIST1H2BM	XP_002697550.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y
Molecular cell23(4)607-18(2006 Aug)

Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II.
Pavri R, Zhu B, Li G, Trojer P, Mandal S, Shilatifard A, Reinberg D
Cell125(4)703-17(2006 May)

Antimicrobial polypeptides of the human colonic epithelium.
Howell SJ, Wilk D, Yadav SP, Bevins CL
Peptides24(11)1763-70(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Histone H2B antimicrobial peptides exhibit antibacterial activity and contribute to the formation of the antimicrobial barrier of the colonic epithelium
Title: Antimicrobial polypeptides of the human colonic epithelium.

The following H2BC12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H2BC12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55800	NM_080593.2 Latest version!	Homo sapiens H2B clustered histone 12 (H2BC12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu55800	NM_001312653.2 Latest version!	Homo sapiens H2B clustered histone 12 (H2BC12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu55800	NM_001312653.1	Homo sapiens histone cluster 1 H2B family member k (HIST1H2BK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H2BC12 ( NM_001312653.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001299582.1 Homo sapiens histone cluster 1 H2B family member k (HIST1H2BK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55800
Clone ID Related Accession (Same CDS sequence)	NM_001312653.1 , NM_001312653.2 , NM_080593.2
Accession Version	NM_001312653.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-25
Organism	Homo sapiens(human)
Product	histone H2B type 1-K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL021807.2. Transcript Variant: This variant (1) contains a palindromic termination sequence instead of a polyA signal and tail. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END##

ATGCCGGAAC CAGCGAAGTC CGCTCCCGCG CCCAAGAAGG GCTCGAAGAA AGCCGTGACT 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA 
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCCTC TAAGGCCATG 
GGAATCATGA ACTCCTTCGT CAACGACATC TTCGAACGCA TCGCGGGTGA GGCTTCCCGC 
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC 
CTGCTGCTGC CCGGGGAGTT GGCCAAGCAC GCCGTGTCCG AGGGCACCAA GGCCGTCACC 
AAGTACACCA GCGCTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299582.1
CDS	61..441
Translation	MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

Target ORF information:

RefSeq Version	NM_001312653.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histone cluster 1 H2B family member k (HIST1H2BK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312653.1

ORF Insert Sequence:

ATGCCGGAAC CAGCGAAGTC CGCTCCCGCG CCCAAGAAGG GCTCGAAGAA AGCCGTGACT 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA 
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCCTC TAAGGCCATG 
GGAATCATGA ACTCCTTCGT CAACGACATC TTCGAACGCA TCGCGGGTGA GGCTTCCCGC 
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC 
CTGCTGCTGC CCGGGGAGTT GGCCAAGCAC GCCGTGTCCG AGGGCACCAA GGCCGTCACC 
AAGTACACCA GCGCTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H2BC12 ( NM_001312653.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001299582.1 Homo sapiens H2B clustered histone 12 (H2BC12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55800
Clone ID Related Accession (Same CDS sequence)	NM_001312653.1 , NM_001312653.2 , NM_080593.2
Accession Version	NM_001312653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-02
Organism	Homo sapiens(human)
Product	histone H2B type 1-K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL021807.2. On Dec 3, 2019 this sequence version replaced NM_001312653.1. Transcript Variant: This variant (1) contains a palindromic termination sequence instead of a polyA signal and tail. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AK292062.1, DA964711.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END##

ATGCCGGAAC CAGCGAAGTC CGCTCCCGCG CCCAAGAAGG GCTCGAAGAA AGCCGTGACT 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA 
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCCTC TAAGGCCATG 
GGAATCATGA ACTCCTTCGT CAACGACATC TTCGAACGCA TCGCGGGTGA GGCTTCCCGC 
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC 
CTGCTGCTGC CCGGGGAGTT GGCCAAGCAC GCCGTGTCCG AGGGCACCAA GGCCGTCACC 
AAGTACACCA GCGCTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299582.1
CDS	58..438
Translation	MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

Target ORF information:

RefSeq Version	NM_001312653.2
Organism	Homo sapiens(human)
Definition	Homo sapiens H2B clustered histone 12 (H2BC12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312653.2

ORF Insert Sequence:

ATGCCGGAAC CAGCGAAGTC CGCTCCCGCG CCCAAGAAGG GCTCGAAGAA AGCCGTGACT 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA 
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCCTC TAAGGCCATG 
GGAATCATGA ACTCCTTCGT CAACGACATC TTCGAACGCA TCGCGGGTGA GGCTTCCCGC 
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC 
CTGCTGCTGC CCGGGGAGTT GGCCAAGCAC GCCGTGTCCG AGGGCACCAA GGCCGTCACC 
AAGTACACCA GCGCTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H2BC12 ( NM_080593.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_542160.1 Homo sapiens H2B clustered histone 12 (H2BC12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55800
Clone ID Related Accession (Same CDS sequence)	NM_001312653.1 , NM_001312653.2 , NM_080593.2
Accession Version	NM_080593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	histone H2B type 1-K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA873786.1 and BC000893.1. On Jul 29, 2010 this sequence version replaced NM_080593.1. Transcript Variant: This variant (2) differs from variant 1 in its 3' UTR which contains a polyA signal and site. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000893.1, SRR1163658.92313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 15019208 replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGAAC CAGCGAAGTC CGCTCCCGCG CCCAAGAAGG GCTCGAAGAA AGCCGTGACT 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA 
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCCTC TAAGGCCATG 
GGAATCATGA ACTCCTTCGT CAACGACATC TTCGAACGCA TCGCGGGTGA GGCTTCCCGC 
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC 
CTGCTGCTGC CCGGGGAGTT GGCCAAGCAC GCCGTGTCCG AGGGCACCAA GGCCGTCACC 
AAGTACACCA GCGCTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542160.1
CDS	61..441
Translation	MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

Target ORF information:

RefSeq Version	NM_080593.2
Organism	Homo sapiens(human)
Definition	Homo sapiens H2B clustered histone 12 (H2BC12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080593.2

ORF Insert Sequence:

ATGCCGGAAC CAGCGAAGTC CGCTCCCGCG CCCAAGAAGG GCTCGAAGAA AGCCGTGACT 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA 
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCCTC TAAGGCCATG 
GGAATCATGA ACTCCTTCGT CAACGACATC TTCGAACGCA TCGCGGGTGA GGCTTCCCGC 
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC 
CTGCTGCTGC CCGGGGAGTT GGCCAAGCAC GCCGTGTCCG AGGGCACCAA GGCCGTCACC 
AAGTACACCA GCGCTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Molecular cell23(4)607-18(2006 Aug)
Kim SC,Sprung R,Chen Y,Xu Y,Ball H,Pei J,Cheng T,Kho Y,Xiao H,Xiao L,Grishin NV,White M,Yang XJ,Zhao Y

Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II.
Cell125(4)703-17(2006 May)
Pavri R,Zhu B,Li G,Trojer P,Mandal S,Shilatifard A,Reinberg D

Antimicrobial polypeptides of the human colonic epithelium.
Peptides24(11)1763-70(2003 Nov)
Howell SJ,Wilk D,Yadav SP,Bevins CL

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H2BC12 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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