ATG4D cDNA ORF clone, Homo sapiens(human)

The following ATG4D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATG4D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu09708 NM_032885.6
Latest version!
Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu09702 NM_001281504.2
Latest version!
Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu51874 XM_006722927.1
Latest version!
Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
View Old Accession Versions >>
OHu09708 NM_032885.5 Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu09702 NM_001281504.1 Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu09708
    Clone ID Related Accession (Same CDS sequence) NM_032885.5 , NM_032885.6
    Accession Version NM_032885.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-01
    Organism Homo sapiens(human)
    Product cysteine protease ATG4D isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY063028.1, BC068992.1 and BM671285.1. On Jul 31, 2013 this sequence version replaced NM_032885.4. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC068992.1, SRR1163657.334111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 22441018 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAACTCAG TGTCGCCGGC CGCCGCGCAG TACCGGAGCA GCAGCCCGGA GGACGCGCGC 
    CGCCGGCCCG AGGCCCGCAG GCCGCGGGGT CCCAGAGGCC CAGACCCCAA CGGCCTGGGG
    CCTTCCGGAG CCAGCGGCCC CGCTCTTGGC TCTCCCGGGG CTGGCCCGAG TGAGCCGGAC
    GAAGTGGACA AGTTCAAGGC CAAGTTCCTG ACAGCCTGGA ACAACGTCAA GTACGGTTGG
    GTGGTTAAAA GCCGGACCAG CTTTAGCAAG ATCTCCAGCA TCCACCTCTG TGGCCGCCGC
    TACCGTTTCG AGGGCGAGGG TGACATACAG CGTTTCCAGC GGGACTTTGT GTCCCGCCTG
    TGGCTCACAT ACCGCCGGGA CTTCCCGCCC CTTCCTGGGG GCTGCCTGAC CTCGGACTGT
    GGCTGGGGGT GCATGTTACG CAGCGGCCAG ATGATGCTGG CACAGGGCCT TCTGCTGCAT
    TTCCTGCCCA GAGACTGGAC ATGGGCCGAG GGCATGGGCC TGGGCCCCCC TGAGCTGTCA
    GGGTCAGCCT CTCCCAGCCG GTACCATGGG CCTGCCCGCT GGATGCCCCC ACGCTGGGCC
    CAGGGTGCCC CTGAGCTGGA GCAGGAACGC CGGCACCGGC AGATTGTGTC CTGGTTCGCC
    GACCACCCCC GGGCCCCCTT TGGCCTACAC CGGCTGGTGG AGCTTGGGCA GAGCTCAGGC
    AAGAAGGCAG GTGACTGGTA TGGGCCATCG CTAGTGGCAC ACATCCTCAG GAAAGCCGTG
    GAGAGCTGCT CCGACGTCAC CCGCCTGGTG GTGTACGTTT CTCAGGACTG CACAGTGTAC
    AAGGCGGATG TGGCACGCCT GGTGGCCAGG CCAGACCCCA CAGCCGAGTG GAAGTCTGTG
    GTCATCCTGG TGCCCGTGCG ACTGGGTGGC GAGACTCTCA ACCCCGTGTA TGTGCCCTGC
    GTGAAGGAAC TCCTGCGTTG CGAGCTGTGC CTGGGCATCA TGGGTGGGAA ACCGCGACAC
    TCACTGTACT TCATTGGCTA CCAAGATGAC TTCCTGCTGT ACCTGGACCC TCACTACTGC
    CAGCCCACTG TGGATGTCAG CCAGGCCGAC TTCCCCCTGG AGTCCTTCCA CTGCACCTCG
    CCCCGCAAGA TGGCCTTTGC CAAGATGGAC CCAAGCTGTA CCGTGGGCTT CTATGCTGGA
    GACAGGAAGG AGTTTGAGAC ACTCTGCTCA GAGCTGACCA GGGTCCTCAG CTCCTCCTCA
    GCCACAGAGC GGTACCCCAT GTTCACCCTG GCCGAGGGCC ATGCTCAGGA CCACAGCCTG
    GACGACCTCT GCTCCCAGCT CGCCCAGCCC ACACTCCGGC TCCCTCGCAC AGGGCGGCTC
    CTCAGGGCCA AACGCCCCAG CTCTGAGGAC TTTGTGTTTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_116274.3
    CDS198..1622
    Translation

    Target ORF information:

    RefSeq Version NM_032885.5
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_032885.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAACTCAG TGTCGCCGGC CGCCGCGCAG TACCGGAGCA GCAGCCCGGA GGACGCGCGC 
    CGCCGGCCCG AGGCCCGCAG GCCGCGGGGT CCCAGAGGCC CAGACCCCAA CGGCCTGGGG
    CCTTCCGGAG CCAGCGGCCC CGCTCTTGGC TCTCCCGGGG CTGGCCCGAG TGAGCCGGAC
    GAAGTGGACA AGTTCAAGGC CAAGTTCCTG ACAGCCTGGA ACAACGTCAA GTACGGTTGG
    GTGGTTAAAA GCCGGACCAG CTTTAGCAAG ATCTCCAGCA TCCACCTCTG TGGCCGCCGC
    TACCGTTTCG AGGGCGAGGG TGACATACAG CGTTTCCAGC GGGACTTTGT GTCCCGCCTG
    TGGCTCACAT ACCGCCGGGA CTTCCCGCCC CTTCCTGGGG GCTGCCTGAC CTCGGACTGT
    GGCTGGGGGT GCATGTTACG CAGCGGCCAG ATGATGCTGG CACAGGGCCT TCTGCTGCAT
    TTCCTGCCCA GAGACTGGAC ATGGGCCGAG GGCATGGGCC TGGGCCCCCC TGAGCTGTCA
    GGGTCAGCCT CTCCCAGCCG GTACCATGGG CCTGCCCGCT GGATGCCCCC ACGCTGGGCC
    CAGGGTGCCC CTGAGCTGGA GCAGGAACGC CGGCACCGGC AGATTGTGTC CTGGTTCGCC
    GACCACCCCC GGGCCCCCTT TGGCCTACAC CGGCTGGTGG AGCTTGGGCA GAGCTCAGGC
    AAGAAGGCAG GTGACTGGTA TGGGCCATCG CTAGTGGCAC ACATCCTCAG GAAAGCCGTG
    GAGAGCTGCT CCGACGTCAC CCGCCTGGTG GTGTACGTTT CTCAGGACTG CACAGTGTAC
    AAGGCGGATG TGGCACGCCT GGTGGCCAGG CCAGACCCCA CAGCCGAGTG GAAGTCTGTG
    GTCATCCTGG TGCCCGTGCG ACTGGGTGGC GAGACTCTCA ACCCCGTGTA TGTGCCCTGC
    GTGAAGGAAC TCCTGCGTTG CGAGCTGTGC CTGGGCATCA TGGGTGGGAA ACCGCGACAC
    TCACTGTACT TCATTGGCTA CCAAGATGAC TTCCTGCTGT ACCTGGACCC TCACTACTGC
    CAGCCCACTG TGGATGTCAG CCAGGCCGAC TTCCCCCTGG AGTCCTTCCA CTGCACCTCG
    CCCCGCAAGA TGGCCTTTGC CAAGATGGAC CCAAGCTGTA CCGTGGGCTT CTATGCTGGA
    GACAGGAAGG AGTTTGAGAC ACTCTGCTCA GAGCTGACCA GGGTCCTCAG CTCCTCCTCA
    GCCACAGAGC GGTACCCCAT GTTCACCCTG GCCGAGGGCC ATGCTCAGGA CCACAGCCTG
    GACGACCTCT GCTCCCAGCT CGCCCAGCCC ACACTCCGGC TCCCTCGCAC AGGGCGGCTC
    CTCAGGGCCA AACGCCCCAG CTCTGAGGAC TTTGTGTTTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09708
    Clone ID Related Accession (Same CDS sequence) NM_032885.5 , NM_032885.6
    Accession Version NM_032885.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-01
    Organism Homo sapiens(human)
    Product cysteine protease ATG4D isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY063028.1 and BC068992.1. On Nov 22, 2018 this sequence version replaced NM_032885.5. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR3476690.908948.1, AK056210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 22441018 MANE Ensembl match :: ENST00000309469.9/ ENSP00000311318.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAACTCAG TGTCGCCGGC CGCCGCGCAG TACCGGAGCA GCAGCCCGGA GGACGCGCGC 
    CGCCGGCCCG AGGCCCGCAG GCCGCGGGGT CCCAGAGGCC CAGACCCCAA CGGCCTGGGG
    CCTTCCGGAG CCAGCGGCCC CGCTCTTGGC TCTCCCGGGG CTGGCCCGAG TGAGCCGGAC
    GAAGTGGACA AGTTCAAGGC CAAGTTCCTG ACAGCCTGGA ACAACGTCAA GTACGGTTGG
    GTGGTTAAAA GCCGGACCAG CTTTAGCAAG ATCTCCAGCA TCCACCTCTG TGGCCGCCGC
    TACCGTTTCG AGGGCGAGGG TGACATACAG CGTTTCCAGC GGGACTTTGT GTCCCGCCTG
    TGGCTCACAT ACCGCCGGGA CTTCCCGCCC CTTCCTGGGG GCTGCCTGAC CTCGGACTGT
    GGCTGGGGGT GCATGTTACG CAGCGGCCAG ATGATGCTGG CACAGGGCCT TCTGCTGCAT
    TTCCTGCCCA GAGACTGGAC ATGGGCCGAG GGCATGGGCC TGGGCCCCCC TGAGCTGTCA
    GGGTCAGCCT CTCCCAGCCG GTACCATGGG CCTGCCCGCT GGATGCCCCC ACGCTGGGCC
    CAGGGTGCCC CTGAGCTGGA GCAGGAACGC CGGCACCGGC AGATTGTGTC CTGGTTCGCC
    GACCACCCCC GGGCCCCCTT TGGCCTACAC CGGCTGGTGG AGCTTGGGCA GAGCTCAGGC
    AAGAAGGCAG GTGACTGGTA TGGGCCATCG CTAGTGGCAC ACATCCTCAG GAAAGCCGTG
    GAGAGCTGCT CCGACGTCAC CCGCCTGGTG GTGTACGTTT CTCAGGACTG CACAGTGTAC
    AAGGCGGATG TGGCACGCCT GGTGGCCAGG CCAGACCCCA CAGCCGAGTG GAAGTCTGTG
    GTCATCCTGG TGCCCGTGCG ACTGGGTGGC GAGACTCTCA ACCCCGTGTA TGTGCCCTGC
    GTGAAGGAAC TCCTGCGTTG CGAGCTGTGC CTGGGCATCA TGGGTGGGAA ACCGCGACAC
    TCACTGTACT TCATTGGCTA CCAAGATGAC TTCCTGCTGT ACCTGGACCC TCACTACTGC
    CAGCCCACTG TGGATGTCAG CCAGGCCGAC TTCCCCCTGG AGTCCTTCCA CTGCACCTCG
    CCCCGCAAGA TGGCCTTTGC CAAGATGGAC CCAAGCTGTA CCGTGGGCTT CTATGCTGGA
    GACAGGAAGG AGTTTGAGAC ACTCTGCTCA GAGCTGACCA GGGTCCTCAG CTCCTCCTCA
    GCCACAGAGC GGTACCCCAT GTTCACCCTG GCCGAGGGCC ATGCTCAGGA CCACAGCCTG
    GACGACCTCT GCTCCCAGCT CGCCCAGCCC ACACTCCGGC TCCCTCGCAC AGGGCGGCTC
    CTCAGGGCCA AACGCCCCAG CTCTGAGGAC TTTGTGTTTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_116274.3
    CDS188..1612
    Translation

    Target ORF information:

    RefSeq Version NM_032885.6
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_032885.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAACTCAG TGTCGCCGGC CGCCGCGCAG TACCGGAGCA GCAGCCCGGA GGACGCGCGC 
    CGCCGGCCCG AGGCCCGCAG GCCGCGGGGT CCCAGAGGCC CAGACCCCAA CGGCCTGGGG
    CCTTCCGGAG CCAGCGGCCC CGCTCTTGGC TCTCCCGGGG CTGGCCCGAG TGAGCCGGAC
    GAAGTGGACA AGTTCAAGGC CAAGTTCCTG ACAGCCTGGA ACAACGTCAA GTACGGTTGG
    GTGGTTAAAA GCCGGACCAG CTTTAGCAAG ATCTCCAGCA TCCACCTCTG TGGCCGCCGC
    TACCGTTTCG AGGGCGAGGG TGACATACAG CGTTTCCAGC GGGACTTTGT GTCCCGCCTG
    TGGCTCACAT ACCGCCGGGA CTTCCCGCCC CTTCCTGGGG GCTGCCTGAC CTCGGACTGT
    GGCTGGGGGT GCATGTTACG CAGCGGCCAG ATGATGCTGG CACAGGGCCT TCTGCTGCAT
    TTCCTGCCCA GAGACTGGAC ATGGGCCGAG GGCATGGGCC TGGGCCCCCC TGAGCTGTCA
    GGGTCAGCCT CTCCCAGCCG GTACCATGGG CCTGCCCGCT GGATGCCCCC ACGCTGGGCC
    CAGGGTGCCC CTGAGCTGGA GCAGGAACGC CGGCACCGGC AGATTGTGTC CTGGTTCGCC
    GACCACCCCC GGGCCCCCTT TGGCCTACAC CGGCTGGTGG AGCTTGGGCA GAGCTCAGGC
    AAGAAGGCAG GTGACTGGTA TGGGCCATCG CTAGTGGCAC ACATCCTCAG GAAAGCCGTG
    GAGAGCTGCT CCGACGTCAC CCGCCTGGTG GTGTACGTTT CTCAGGACTG CACAGTGTAC
    AAGGCGGATG TGGCACGCCT GGTGGCCAGG CCAGACCCCA CAGCCGAGTG GAAGTCTGTG
    GTCATCCTGG TGCCCGTGCG ACTGGGTGGC GAGACTCTCA ACCCCGTGTA TGTGCCCTGC
    GTGAAGGAAC TCCTGCGTTG CGAGCTGTGC CTGGGCATCA TGGGTGGGAA ACCGCGACAC
    TCACTGTACT TCATTGGCTA CCAAGATGAC TTCCTGCTGT ACCTGGACCC TCACTACTGC
    CAGCCCACTG TGGATGTCAG CCAGGCCGAC TTCCCCCTGG AGTCCTTCCA CTGCACCTCG
    CCCCGCAAGA TGGCCTTTGC CAAGATGGAC CCAAGCTGTA CCGTGGGCTT CTATGCTGGA
    GACAGGAAGG AGTTTGAGAC ACTCTGCTCA GAGCTGACCA GGGTCCTCAG CTCCTCCTCA
    GCCACAGAGC GGTACCCCAT GTTCACCCTG GCCGAGGGCC ATGCTCAGGA CCACAGCCTG
    GACGACCTCT GCTCCCAGCT CGCCCAGCCC ACACTCCGGC TCCCTCGCAC AGGGCGGCTC
    CTCAGGGCCA AACGCCCCAG CTCTGAGGAC TTTGTGTTTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09702
    Clone ID Related Accession (Same CDS sequence) NM_001281504.1 , NM_001281504.2
    Accession Version NM_001281504.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product cysteine protease ATG4D isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY063028.1, AK294676.1, BC068992.1 and BM671285.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.156928.1, SRR1163655.511590.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 22441018 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCAAGTGC TTCATCTCGC TGGGCGCTGC CCCTACGTCT CCCCAGGTTG GGTGGTTAAA 
    AGCCGGACCA GCTTTAGCAA GATCTCCAGC ATCCACCTCT GTGGCCGCCG CTACCGTTTC
    GAGGGCGAGG GTGACATACA GCGTTTCCAG CGGGACTTTG TGTCCCGCCT GTGGCTCACA
    TACCGCCGGG ACTTCCCGCC CCTTCCTGGG GGCTGCCTGA CCTCGGACTG TGGCTGGGGG
    TGCATGTTAC GCAGCGGCCA GATGATGCTG GCACAGGGCC TTCTGCTGCA TTTCCTGCCC
    AGAGACTGGA CATGGGCCGA GGGCATGGGC CTGGGCCCCC CTGAGCTGTC AGGGTCAGCC
    TCTCCCAGCC GGTACCATGG GCCTGCCCGC TGGATGCCCC CACGCTGGGC CCAGGGTGCC
    CCTGAGCTGG AGCAGGAACG CCGGCACCGG CAGATTGTGT CCTGGTTCGC CGACCACCCC
    CGGGCCCCCT TTGGCCTACA CCGGCTGGTG GAGCTTGGGC AGAGCTCAGG CAAGAAGGCA
    GGTGACTGGT ATGGGCCATC GCTAGTGGCA CACATCCTCA GGAAAGCCGT GGAGAGCTGC
    TCCGACGTCA CCCGCCTGGT GGTGTACGTT TCTCAGGACT GCACAGTGTA CAAGGCGGAT
    GTGGCACGCC TGGTGGCCAG GCCAGACCCC ACAGCCGAGT GGAAGTCTGT GGTCATCCTG
    GTGCCCGTGC GACTGGGTGG CGAGACTCTC AACCCCGTGT ATGTGCCCTG CGTGAAGGAA
    CTCCTGCGTT GCGAGCTGTG CCTGGGCATC ATGGGTGGGA AACCGCGACA CTCACTGTAC
    TTCATTGGCT ACCAAGATGA CTTCCTGCTG TACCTGGACC CTCACTACTG CCAGCCCACT
    GTGGATGTCA GCCAGGCCGA CTTCCCCCTG GAGTCCTTCC ACTGCACCTC GCCCCGCAAG
    ATGGCCTTTG CCAAGATGGA CCCAAGCTGT ACCGTGGGCT TCTATGCTGG AGACAGGAAG
    GAGTTTGAGA CACTCTGCTC AGAGCTGACC AGGGTCCTCA GCTCCTCCTC AGCCACAGAG
    CGGTACCCCA TGTTCACCCT GGCCGAGGGC CATGCTCAGG ACCACAGCCT GGACGACCTC
    TGCTCCCAGC TCGCCCAGCC CACACTCCGG CTCCCTCGCA CAGGGCGGCT CCTCAGGGCC
    AAACGCCCCA GCTCTGAGGA CTTTGTGTTT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001268433.1
    CDS461..1696
    Translation

    Target ORF information:

    RefSeq Version NM_001281504.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001281504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCAAGTGC TTCATCTCGC TGGGCGCTGC CCCTACGTCT CCCCAGGTTG GGTGGTTAAA 
    AGCCGGACCA GCTTTAGCAA GATCTCCAGC ATCCACCTCT GTGGCCGCCG CTACCGTTTC
    GAGGGCGAGG GTGACATACA GCGTTTCCAG CGGGACTTTG TGTCCCGCCT GTGGCTCACA
    TACCGCCGGG ACTTCCCGCC CCTTCCTGGG GGCTGCCTGA CCTCGGACTG TGGCTGGGGG
    TGCATGTTAC GCAGCGGCCA GATGATGCTG GCACAGGGCC TTCTGCTGCA TTTCCTGCCC
    AGAGACTGGA CATGGGCCGA GGGCATGGGC CTGGGCCCCC CTGAGCTGTC AGGGTCAGCC
    TCTCCCAGCC GGTACCATGG GCCTGCCCGC TGGATGCCCC CACGCTGGGC CCAGGGTGCC
    CCTGAGCTGG AGCAGGAACG CCGGCACCGG CAGATTGTGT CCTGGTTCGC CGACCACCCC
    CGGGCCCCCT TTGGCCTACA CCGGCTGGTG GAGCTTGGGC AGAGCTCAGG CAAGAAGGCA
    GGTGACTGGT ATGGGCCATC GCTAGTGGCA CACATCCTCA GGAAAGCCGT GGAGAGCTGC
    TCCGACGTCA CCCGCCTGGT GGTGTACGTT TCTCAGGACT GCACAGTGTA CAAGGCGGAT
    GTGGCACGCC TGGTGGCCAG GCCAGACCCC ACAGCCGAGT GGAAGTCTGT GGTCATCCTG
    GTGCCCGTGC GACTGGGTGG CGAGACTCTC AACCCCGTGT ATGTGCCCTG CGTGAAGGAA
    CTCCTGCGTT GCGAGCTGTG CCTGGGCATC ATGGGTGGGA AACCGCGACA CTCACTGTAC
    TTCATTGGCT ACCAAGATGA CTTCCTGCTG TACCTGGACC CTCACTACTG CCAGCCCACT
    GTGGATGTCA GCCAGGCCGA CTTCCCCCTG GAGTCCTTCC ACTGCACCTC GCCCCGCAAG
    ATGGCCTTTG CCAAGATGGA CCCAAGCTGT ACCGTGGGCT TCTATGCTGG AGACAGGAAG
    GAGTTTGAGA CACTCTGCTC AGAGCTGACC AGGGTCCTCA GCTCCTCCTC AGCCACAGAG
    CGGTACCCCA TGTTCACCCT GGCCGAGGGC CATGCTCAGG ACCACAGCCT GGACGACCTC
    TGCTCCCAGC TCGCCCAGCC CACACTCCGG CTCCCTCGCA CAGGGCGGCT CCTCAGGGCC
    AAACGCCCCA GCTCTGAGGA CTTTGTGTTT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09702
    Clone ID Related Accession (Same CDS sequence) NM_001281504.1 , NM_001281504.2
    Accession Version NM_001281504.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product cysteine protease ATG4D isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK294676.1 and BC068992.1. On May 31, 2019 this sequence version replaced NM_001281504.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.156928.1, SRR3476690.660180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 22441018 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCAAGTGC TTCATCTCGC TGGGCGCTGC CCCTACGTCT CCCCAGGTTG GGTGGTTAAA 
    AGCCGGACCA GCTTTAGCAA GATCTCCAGC ATCCACCTCT GTGGCCGCCG CTACCGTTTC
    GAGGGCGAGG GTGACATACA GCGTTTCCAG CGGGACTTTG TGTCCCGCCT GTGGCTCACA
    TACCGCCGGG ACTTCCCGCC CCTTCCTGGG GGCTGCCTGA CCTCGGACTG TGGCTGGGGG
    TGCATGTTAC GCAGCGGCCA GATGATGCTG GCACAGGGCC TTCTGCTGCA TTTCCTGCCC
    AGAGACTGGA CATGGGCCGA GGGCATGGGC CTGGGCCCCC CTGAGCTGTC AGGGTCAGCC
    TCTCCCAGCC GGTACCATGG GCCTGCCCGC TGGATGCCCC CACGCTGGGC CCAGGGTGCC
    CCTGAGCTGG AGCAGGAACG CCGGCACCGG CAGATTGTGT CCTGGTTCGC CGACCACCCC
    CGGGCCCCCT TTGGCCTACA CCGGCTGGTG GAGCTTGGGC AGAGCTCAGG CAAGAAGGCA
    GGTGACTGGT ATGGGCCATC GCTAGTGGCA CACATCCTCA GGAAAGCCGT GGAGAGCTGC
    TCCGACGTCA CCCGCCTGGT GGTGTACGTT TCTCAGGACT GCACAGTGTA CAAGGCGGAT
    GTGGCACGCC TGGTGGCCAG GCCAGACCCC ACAGCCGAGT GGAAGTCTGT GGTCATCCTG
    GTGCCCGTGC GACTGGGTGG CGAGACTCTC AACCCCGTGT ATGTGCCCTG CGTGAAGGAA
    CTCCTGCGTT GCGAGCTGTG CCTGGGCATC ATGGGTGGGA AACCGCGACA CTCACTGTAC
    TTCATTGGCT ACCAAGATGA CTTCCTGCTG TACCTGGACC CTCACTACTG CCAGCCCACT
    GTGGATGTCA GCCAGGCCGA CTTCCCCCTG GAGTCCTTCC ACTGCACCTC GCCCCGCAAG
    ATGGCCTTTG CCAAGATGGA CCCAAGCTGT ACCGTGGGCT TCTATGCTGG AGACAGGAAG
    GAGTTTGAGA CACTCTGCTC AGAGCTGACC AGGGTCCTCA GCTCCTCCTC AGCCACAGAG
    CGGTACCCCA TGTTCACCCT GGCCGAGGGC CATGCTCAGG ACCACAGCCT GGACGACCTC
    TGCTCCCAGC TCGCCCAGCC CACACTCCGG CTCCCTCGCA CAGGGCGGCT CCTCAGGGCC
    AAACGCCCCA GCTCTGAGGA CTTTGTGTTT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001268433.1
    CDS451..1686
    Translation

    Target ORF information:

    RefSeq Version NM_001281504.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001281504.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCAAGTGC TTCATCTCGC TGGGCGCTGC CCCTACGTCT CCCCAGGTTG GGTGGTTAAA 
    AGCCGGACCA GCTTTAGCAA GATCTCCAGC ATCCACCTCT GTGGCCGCCG CTACCGTTTC
    GAGGGCGAGG GTGACATACA GCGTTTCCAG CGGGACTTTG TGTCCCGCCT GTGGCTCACA
    TACCGCCGGG ACTTCCCGCC CCTTCCTGGG GGCTGCCTGA CCTCGGACTG TGGCTGGGGG
    TGCATGTTAC GCAGCGGCCA GATGATGCTG GCACAGGGCC TTCTGCTGCA TTTCCTGCCC
    AGAGACTGGA CATGGGCCGA GGGCATGGGC CTGGGCCCCC CTGAGCTGTC AGGGTCAGCC
    TCTCCCAGCC GGTACCATGG GCCTGCCCGC TGGATGCCCC CACGCTGGGC CCAGGGTGCC
    CCTGAGCTGG AGCAGGAACG CCGGCACCGG CAGATTGTGT CCTGGTTCGC CGACCACCCC
    CGGGCCCCCT TTGGCCTACA CCGGCTGGTG GAGCTTGGGC AGAGCTCAGG CAAGAAGGCA
    GGTGACTGGT ATGGGCCATC GCTAGTGGCA CACATCCTCA GGAAAGCCGT GGAGAGCTGC
    TCCGACGTCA CCCGCCTGGT GGTGTACGTT TCTCAGGACT GCACAGTGTA CAAGGCGGAT
    GTGGCACGCC TGGTGGCCAG GCCAGACCCC ACAGCCGAGT GGAAGTCTGT GGTCATCCTG
    GTGCCCGTGC GACTGGGTGG CGAGACTCTC AACCCCGTGT ATGTGCCCTG CGTGAAGGAA
    CTCCTGCGTT GCGAGCTGTG CCTGGGCATC ATGGGTGGGA AACCGCGACA CTCACTGTAC
    TTCATTGGCT ACCAAGATGA CTTCCTGCTG TACCTGGACC CTCACTACTG CCAGCCCACT
    GTGGATGTCA GCCAGGCCGA CTTCCCCCTG GAGTCCTTCC ACTGCACCTC GCCCCGCAAG
    ATGGCCTTTG CCAAGATGGA CCCAAGCTGT ACCGTGGGCT TCTATGCTGG AGACAGGAAG
    GAGTTTGAGA CACTCTGCTC AGAGCTGACC AGGGTCCTCA GCTCCTCCTC AGCCACAGAG
    CGGTACCCCA TGTTCACCCT GGCCGAGGGC CATGCTCAGG ACCACAGCCT GGACGACCTC
    TGCTCCCAGC TCGCCCAGCC CACACTCCGG CTCCCTCGCA CAGGGCGGCT CCTCAGGGCC
    AAACGCCCCA GCTCTGAGGA CTTTGTGTTT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51874
    Clone ID Related Accession (Same CDS sequence) XM_006722927.1
    Accession Version XM_006722927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine protease ATG4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAACTCAG TGTCGCCGGC CGCCGCGCAG TACCGGAGCA GCAGCCCGGA GGACGCGCGC 
    CGCCGGCCCG AGGCCCGCAG GCCGCGGGGT CCCAGAGGCC CAGACCCCAA CGGCCTGGGG
    CCTTCCGGAG CCAGCGGCCC CGCTCTTGGC TCTCCCGGGG CTGGCCCGAG TGAGCCGGAC
    GAAGTGGACA AGTTCAAGGC CAAGTTCCTG ACAGCCTGGA ACAACGTCAA GTACGGTTGG
    GTGGTTAAAA GCCGGACCAG CTTTAGCAAG ATCTCCAGCA TCCACCTCTG TGGCCGCCGC
    TACCGTTTCG AGGGCGAGGG TGACATACAG CGTTTCCAGC GGGACTTTGT GTCCCGCCTG
    TGGCTCACAT ACCGCCGGGA CTTCCCGCCC CTTCCTGGGG GCTGCCTGAC CTCGGACTGT
    GGCTGGGGGT GCATGTTACG CAGCGGCCAG ATGATGCTGG CACAGGGCCT TCTGCTGCAT
    TTCCTGCCCA GAGTGTACAA GGCGGATGTG GCACGCCTGG TGGCCAGGCC AGACCCCACA
    GCCGAGTGGA AGTCTGTGGT CATCCTGGTG CCCGTGCGAC TGGGTGGCGA GACTCTCAAC
    CCCGTGTATG TGCCCTGCGT GAAGGAACTC CTGCGTTGCG AGCTGTGCCT GGGCATCATG
    GGTGGGAAAC CGCGACACTC ACTGTACTTC ATTGGCTACC AAGATGACTT CCTGCTGTAC
    CTGGACCCTC ACTACTGCCA GCCCACTGTG GATGTCAGCC AGGCCGACTT CCCCCTGGAG
    TCCTTCCACT GCACCTCGCC CCGCAAGATG GCCTTTGCCA AGATGGACCC AAGCTGTACC
    GTGGGCTTCT ATGCTGGAGA CAGGAAGGAG TTTGAGACAC TCTGCTCAGA GCTGACCAGG
    GTCCTCAGCT CCTCCTCAGC CACAGAGCGG TACCCCATGT TCACCCTGGC CGAGGGCCAT
    GCTCAGGACC ACAGCCTGGA CGACCTCTGC TCCCAGCTCG CCCAGCCCAC ACTCCGGCTC
    CCTCGCACAG GGCGGCTCCT CAGGGCCAAA CGCCCCAGCT CTGAGGACTT TGTGTTTTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722990.1
    CDS195..1277
    Translation

    Target ORF information:

    RefSeq Version XM_006722927.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 4D cysteine peptidase (ATG4D), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAACTCAG TGTCGCCGGC CGCCGCGCAG TACCGGAGCA GCAGCCCGGA GGACGCGCGC 
    CGCCGGCCCG AGGCCCGCAG GCCGCGGGGT CCCAGAGGCC CAGACCCCAA CGGCCTGGGG
    CCTTCCGGAG CCAGCGGCCC CGCTCTTGGC TCTCCCGGGG CTGGCCCGAG TGAGCCGGAC
    GAAGTGGACA AGTTCAAGGC CAAGTTCCTG ACAGCCTGGA ACAACGTCAA GTACGGTTGG
    GTGGTTAAAA GCCGGACCAG CTTTAGCAAG ATCTCCAGCA TCCACCTCTG TGGCCGCCGC
    TACCGTTTCG AGGGCGAGGG TGACATACAG CGTTTCCAGC GGGACTTTGT GTCCCGCCTG
    TGGCTCACAT ACCGCCGGGA CTTCCCGCCC CTTCCTGGGG GCTGCCTGAC CTCGGACTGT
    GGCTGGGGGT GCATGTTACG CAGCGGCCAG ATGATGCTGG CACAGGGCCT TCTGCTGCAT
    TTCCTGCCCA GAGTGTACAA GGCGGATGTG GCACGCCTGG TGGCCAGGCC AGACCCCACA
    GCCGAGTGGA AGTCTGTGGT CATCCTGGTG CCCGTGCGAC TGGGTGGCGA GACTCTCAAC
    CCCGTGTATG TGCCCTGCGT GAAGGAACTC CTGCGTTGCG AGCTGTGCCT GGGCATCATG
    GGTGGGAAAC CGCGACACTC ACTGTACTTC ATTGGCTACC AAGATGACTT CCTGCTGTAC
    CTGGACCCTC ACTACTGCCA GCCCACTGTG GATGTCAGCC AGGCCGACTT CCCCCTGGAG
    TCCTTCCACT GCACCTCGCC CCGCAAGATG GCCTTTGCCA AGATGGACCC AAGCTGTACC
    GTGGGCTTCT ATGCTGGAGA CAGGAAGGAG TTTGAGACAC TCTGCTCAGA GCTGACCAGG
    GTCCTCAGCT CCTCCTCAGC CACAGAGCGG TACCCCATGT TCACCCTGGC CGAGGGCCAT
    GCTCAGGACC ACAGCCTGGA CGACCTCTGC TCCCAGCTCG CCCAGCCCAC ACTCCGGCTC
    CCTCGCACAG GGCGGCTCCT CAGGGCCAAA CGCCCCAGCT CTGAGGACTT TGTGTTTTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.