UBASH3B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBASH3B
Entrez Gene ID	84959
Full Name	ubiquitin associated and SH3 domain containing B
Synonyms	STS-1,STS1,TULA-2,TULA2,p70
General protein information	Preferred Names ubiquitin associated and SH3 domain containing B Names ubiquitin-associated and SH3 domain-containing protein B Cbl-interacting protein Sts-1 SH3 domain-containing 70 kDa protein, suppressor of T-cell receptor signaling 1, nm23-phosphorylated unknown substrate T-cell ubiquitin ligand 2 cbl-interacting protein p70 nm23-phosphorylated unknown substrate suppressor of T-cell receptor signaling 1 tyrosine-protein phosphatase STS1/TULA2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q24.1
Summary	This gene encodes a protein that contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. The encoded protein was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor. [provided by RefSeq, Jul 2008].
Disorder MIM:	609201

mRNA and Protein(s)

mRNA	Protein	Name
NM_032873.4	NP_116262.2	ubiquitin-associated and SH3 domain-containing protein B
NM_001363365.1	NP_001350294.1	ubiquitin-associated and SH3 domain-containing protein B isoform 2
NM_001363365.2	NP_001350294.1	ubiquitin-associated and SH3 domain-containing protein B isoform 2
NM_032873.5	NP_116262.2	ubiquitin-associated and SH3 domain-containing protein B isoform 1
XM_011543041.2	XP_011541343.1	ubiquitin-associated and SH3 domain-containing protein B isoform X2
XM_011543042.1	XP_011541344.1	ubiquitin-associated and SH3 domain-containing protein B isoform X3
XM_005271712.3	XP_005271769.1	ubiquitin-associated and SH3 domain-containing protein B isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	UBASH3B	XP_005619714.1
Xenopus tropicalis (tropical clawed frog)	ubash3b	NP_001096431.1
Pan troglodytes (chimpanzee)	UBASH3B	XP_508828.2
Gallus gallus (chicken)	UBASH3B	XP_004948071.1
Drosophila melanogaster (fruit fly)	CG13604	NP_001262894.1
Homo sapiens (human)	UBASH3B	NP_116262.2
Rattus norvegicus (Norway rat)	Ubash3b	NP_001178721.1
Bos taurus (cattle)	UBASH3B	NP_001193057.1
Danio rerio (zebrafish)	si:ch211-49j11.1	XP_001923885.2
Danio rerio (zebrafish)	zgc:195004	NP_001122227.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002463	XP_312476.5
	UBASH3B	XP_001107573.1
Mus musculus (house mouse)	Ubash3b	NP_789830.1
Caenorhabditis elegans (roundworm)	T07F12.1	NP_508832.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of STS-1 as a novel ShcA-binding protein.
van der Meulen T, Swarts S, Fischer W, van der Geer P
Biochemical and biophysical research communications490(4)1334-1339(2017 09)

UBASH3B/Sts-1-CBL axis regulates myeloid proliferation in human preleukemia induced by AML1-ETO.
Goyama S, Schibler J, Gasilina A, Shrestha M, Lin S, Link KA, Chen J, Whitman SP, Bloomfield CD, Nicolet D, Assi SA, Ptasinska A, Heidenreich O, Bonifer C, Kitamura T, Nassar NN, Mulloy JC
Leukemia30(3)728-39(2016 Mar)

Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules.
Krupina K, Kleiss C, Metzger T, Fournane S, Schmucker S, Hofmann K, Fischer B, Paul N, Porter IM, Raffelsberger W, Poch O, Swedlow JR, Brino L, Sumara I
Developmental cell36(1)63-78(2016 Jan)

Anti-miR-148a regulates platelet Fc?RIIA signaling and decreases thrombosis in vivo in mice.
Zhou Y, Abraham S, Andre P, Edelstein LC, Shaw CA, Dangelmaier CA, Tsygankov AY, Kunapuli SP, Bray PF, McKenzie SE
Blood126(26)2871-81(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results show that the interaction between STS-1 and ShcA is regulated in response to EGF receptor activation.
Title: Identification of STS-1 as a novel ShcA-binding protein.

The study reveals a role of CBL in restricting myeloid proliferation of human AML1-ETOinduced leukemia, and identifies UBASH3B as a potential target for pharmaceutical intervention.
Title: UBASH3B/Sts-1-CBL axis regulates myeloid proliferation in human preleukemia induced by AML1-ETO.

targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells
Title: Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules.

These results show that TULA-2 is a target of miR-148a-3p
Title: Anti-miR-148a regulates platelet Fc?RIIA signaling and decreases thrombosis in vivo in mice.

results demonstrate the importance of STS-1 in regulating IFN-alpha-induced autophagy in B cells
Title: STS-1 promotes IFN-? induced autophagy by activating the JAK1-STAT1 signaling pathway in B cells.

The following UBASH3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBASH3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25000	NM_032873.5 Latest version!	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu73896	NM_001363365.2 Latest version!	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu73895	XM_011543041.2 Latest version!	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu73896	XM_011543042.1 Latest version!	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu45904	XM_005271712.3 Latest version!	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu73896	NM_001363365.1	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu25000	NM_032873.4	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBASH3B ( NM_032873.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_116262.2 Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), mRNA.

Price & Availability↑

CloneID	OHu25000
Clone ID Related Accession (Same CDS sequence)	NM_032873.4 , NM_032873.5
Accession Version	NM_032873.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ubiquitin-associated and SH3 domain-containing protein B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA197468.1, AK075203.1, BC007541.1, AP002762.4 and CR741636.1. On Dec 20, 2007 this sequence version replaced NM_032873.3. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.165220.1, SRR1803612.231648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCAGT ACGGCCACCC CAGTCCGCTC GGCATGGCTG CGAGAGAGGA GCTGTACAGC 
AAAGTCACCC CCCGGAGGAA CCGCCAACAG CGCCCCGGCA CCATCAAGCA TGGATCGGCG 
CTGGACGTGC TCCTCTCCAT GGGGTTCCCC AGAGCCCGCG CACAAAAAGC CTTGGCATCC 
ACGGGAGGAA GAAGTGTTCA GGCAGCATGT GACTGGTTAT TCTCCCATGT CGGTGACCCC 
TTCCTGGATG ACCCCCTGCC CCGGGAGTAC GTCCTCTACC TCCGTCCCAC CGGCCCCTTA 
GCACAGAAGC TTTCCGACTT TTGGCAGCAG TCGAAGCAGA TCTGCGGGAA GAACAAGGCA 
CACAACATCT TCCCCCACAT CACACTCTGC CAGTTCTTTA TGTGCGAGGA CAGCAAGGTG 
GATGCCCTGG GGGAAGCCCT GCAGACCACG GTCAGTCGCT GGAAATGTAA GTTCTCGGCC 
CCGCTGCCCC TGGAGCTCTA TACGTCGTCC AACTTCATCG GCCTCTTTGT AAAGGAAGAC 
AGTGCGGAGG TCCTCAAGAA GTTTGCTGCT GACTTTGCTG CAGAGGCTGC ATCCAAAACC 
GAAGTGCATG TGGAACCTCA TAAGAAGCAG CTACATGTGA CCCTGGCTTA CCACTTCCAA 
GCCAGCCACC TACCCACCCT AGAGAAACTG GCCCAGAACA TTGACGTCAA GCTAGGGTGT 
GACTGGGTGG CTACCATATT TTCTCGGGAT ATCCGATTTG CTAACCATGA GACATTACAG 
GTCATCTACC CCTATACCCC ACAAAATGAC GATGAGCTGG AGCTGGTCCC CGGGGACTTC 
ATCTTCATGT CTCCAATGGA GCAGACCAGC ACCAGCGAGG GTTGGATCTA TGGCACGTCC 
TTAACCACCG GCTGCTCTGG ACTCCTGCCT GAGAATTACA TTACCAAGGC TGATGAATGC 
AGCACCTGGA TATTTCATGG TTCTTATTCA ATCTTAAATA CATCGTCATC CAACTCTCTC 
ACGTTTGGGG ATGGAGTATT GGAGAGGCGG CCTTATGAGG ACCAGGGGCT CGGGGAGACG 
ACTCCTCTTA CTATCATCTG CCAGCCCATG CAGCCGCTGA GGGTCAACAG CCAGCCCGGC 
CCCCAGAAGC GATGCCTTTT TGTGTGTCGG CATGGTGAGA GGATGGATGT TGTGTTTGGG 
AAGTACTGGC TGTCCCAGTG CTTCGATGCC AAAGGCCGCT ACATACGCAC CAACCTGAAC 
ATGCCTCATA GTTTACCTCA GCGGAGTGGT GGTTTCCGAG ATTACGAGAA AGATGCTCCC 
ATCACTGTGT TTGGATGCAT GCAAGCAAGA CTAGTGGGTG AAGCCTTATT AGAGAGCAAT 
ACCATTATCG ATCATGTCTA TTGCTCCCCG TCCCTTCGCT GCGTTCAGAC TGCACACAAT 
ATCTTGAAAG GTTTACAACA AGAAAATCAC TTGAAGATCC GTGTAGAGCC CGGCTTATTT 
GAGTGGACAA AATGGGTTGC TGGGAGCACA TTACCTGCAT GGATACCTCC ATCAGAGTTA 
GCTGCAGCCA ACCTGAGTGT TGATACAACC TACAGACCTC ACATTCCAAT CAGCAAATTA 
GTTGTTTCAG AATCCTATGA TACTTATATC AGTAGAAGTT TCCAAGTAAC AAAAGAAATA 
ATAAGTGAAT GTAAAAGTAA AGGAAATAAC ATCCTGATTG TGGCCCACGC ATCTTCCCTT 
GAAGCGTGTA CCTGCCAACT TCAGGGCCTG TCACCTCAGA ACTCCAAGGA CTTCGTACAA 
ATGGTCCGAA AGATCCCATA TCTGGGATTT TGTTCCTGTG AAGAATTAGG AGAAACTGGA 
ATATGGCAGC TGACAGATCC ACCAATCCTT CCTCTTACCC ATGGACCAAC TGGGGGCTTC 
AACTGGAGAG AGACCTTGCT TCAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116262.2
CDS	361..2310
Translation	MAQYGHPSPLGMAAREELYSKVTPRRNRQQRPGTIKHGSALDVLLSMGFPRARAQKALASTGGRSVQAACDWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYTPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERRPYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTIIDHVYCSPSLRCVQTAHNILKGLQQENHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPISKLVVSESYDTYISRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE

Target ORF information:

RefSeq Version	NM_032873.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032873.4

ORF Insert Sequence:

ATGGCTCAGT ACGGCCACCC CAGTCCGCTC GGCATGGCTG CGAGAGAGGA GCTGTACAGC 
AAAGTCACCC CCCGGAGGAA CCGCCAACAG CGCCCCGGCA CCATCAAGCA TGGATCGGCG 
CTGGACGTGC TCCTCTCCAT GGGGTTCCCC AGAGCCCGCG CACAAAAAGC CTTGGCATCC 
ACGGGAGGAA GAAGTGTTCA GGCAGCATGT GACTGGTTAT TCTCCCATGT CGGTGACCCC 
TTCCTGGATG ACCCCCTGCC CCGGGAGTAC GTCCTCTACC TCCGTCCCAC CGGCCCCTTA 
GCACAGAAGC TTTCCGACTT TTGGCAGCAG TCGAAGCAGA TCTGCGGGAA GAACAAGGCA 
CACAACATCT TCCCCCACAT CACACTCTGC CAGTTCTTTA TGTGCGAGGA CAGCAAGGTG 
GATGCCCTGG GGGAAGCCCT GCAGACCACG GTCAGTCGCT GGAAATGTAA GTTCTCGGCC 
CCGCTGCCCC TGGAGCTCTA TACGTCGTCC AACTTCATCG GCCTCTTTGT AAAGGAAGAC 
AGTGCGGAGG TCCTCAAGAA GTTTGCTGCT GACTTTGCTG CAGAGGCTGC ATCCAAAACC 
GAAGTGCATG TGGAACCTCA TAAGAAGCAG CTACATGTGA CCCTGGCTTA CCACTTCCAA 
GCCAGCCACC TACCCACCCT AGAGAAACTG GCCCAGAACA TTGACGTCAA GCTAGGGTGT 
GACTGGGTGG CTACCATATT TTCTCGGGAT ATCCGATTTG CTAACCATGA GACATTACAG 
GTCATCTACC CCTATACCCC ACAAAATGAC GATGAGCTGG AGCTGGTCCC CGGGGACTTC 
ATCTTCATGT CTCCAATGGA GCAGACCAGC ACCAGCGAGG GTTGGATCTA TGGCACGTCC 
TTAACCACCG GCTGCTCTGG ACTCCTGCCT GAGAATTACA TTACCAAGGC TGATGAATGC 
AGCACCTGGA TATTTCATGG TTCTTATTCA ATCTTAAATA CATCGTCATC CAACTCTCTC 
ACGTTTGGGG ATGGAGTATT GGAGAGGCGG CCTTATGAGG ACCAGGGGCT CGGGGAGACG 
ACTCCTCTTA CTATCATCTG CCAGCCCATG CAGCCGCTGA GGGTCAACAG CCAGCCCGGC 
CCCCAGAAGC GATGCCTTTT TGTGTGTCGG CATGGTGAGA GGATGGATGT TGTGTTTGGG 
AAGTACTGGC TGTCCCAGTG CTTCGATGCC AAAGGCCGCT ACATACGCAC CAACCTGAAC 
ATGCCTCATA GTTTACCTCA GCGGAGTGGT GGTTTCCGAG ATTACGAGAA AGATGCTCCC 
ATCACTGTGT TTGGATGCAT GCAAGCAAGA CTAGTGGGTG AAGCCTTATT AGAGAGCAAT 
ACCATTATCG ATCATGTCTA TTGCTCCCCG TCCCTTCGCT GCGTTCAGAC TGCACACAAT 
ATCTTGAAAG GTTTACAACA AGAAAATCAC TTGAAGATCC GTGTAGAGCC CGGCTTATTT 
GAGTGGACAA AATGGGTTGC TGGGAGCACA TTACCTGCAT GGATACCTCC ATCAGAGTTA 
GCTGCAGCCA ACCTGAGTGT TGATACAACC TACAGACCTC ACATTCCAAT CAGCAAATTA 
GTTGTTTCAG AATCCTATGA TACTTATATC AGTAGAAGTT TCCAAGTAAC AAAAGAAATA 
ATAAGTGAAT GTAAAAGTAA AGGAAATAAC ATCCTGATTG TGGCCCACGC ATCTTCCCTT 
GAAGCGTGTA CCTGCCAACT TCAGGGCCTG TCACCTCAGA ACTCCAAGGA CTTCGTACAA 
ATGGTCCGAA AGATCCCATA TCTGGGATTT TGTTCCTGTG AAGAATTAGG AGAAACTGGA 
ATATGGCAGC TGACAGATCC ACCAATCCTT CCTCTTACCC ATGGACCAAC TGGGGGCTTC 
AACTGGAGAG AGACCTTGCT TCAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBASH3B ( NM_032873.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_116262.2 Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25000
Clone ID Related Accession (Same CDS sequence)	NM_032873.4 , NM_032873.5
Accession Version	NM_032873.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	ubiquitin-associated and SH3 domain-containing protein B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK075203.1, BC007541.1, AP002762.4 and CR741636.1. On Nov 22, 2018 this sequence version replaced NM_032873.4. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK075203.1, SRR1803617.165220.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000284273.6/ ENSP00000284273.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCAGT ACGGCCACCC CAGTCCGCTC GGCATGGCTG CGAGAGAGGA GCTGTACAGC 
AAAGTCACCC CCCGGAGGAA CCGCCAACAG CGCCCCGGCA CCATCAAGCA TGGATCGGCG 
CTGGACGTGC TCCTCTCCAT GGGGTTCCCC AGAGCCCGCG CACAAAAAGC CTTGGCATCC 
ACGGGAGGAA GAAGTGTTCA GGCAGCATGT GACTGGTTAT TCTCCCATGT CGGTGACCCC 
TTCCTGGATG ACCCCCTGCC CCGGGAGTAC GTCCTCTACC TCCGTCCCAC CGGCCCCTTA 
GCACAGAAGC TTTCCGACTT TTGGCAGCAG TCGAAGCAGA TCTGCGGGAA GAACAAGGCA 
CACAACATCT TCCCCCACAT CACACTCTGC CAGTTCTTTA TGTGCGAGGA CAGCAAGGTG 
GATGCCCTGG GGGAAGCCCT GCAGACCACG GTCAGTCGCT GGAAATGTAA GTTCTCGGCC 
CCGCTGCCCC TGGAGCTCTA TACGTCGTCC AACTTCATCG GCCTCTTTGT AAAGGAAGAC 
AGTGCGGAGG TCCTCAAGAA GTTTGCTGCT GACTTTGCTG CAGAGGCTGC ATCCAAAACC 
GAAGTGCATG TGGAACCTCA TAAGAAGCAG CTACATGTGA CCCTGGCTTA CCACTTCCAA 
GCCAGCCACC TACCCACCCT AGAGAAACTG GCCCAGAACA TTGACGTCAA GCTAGGGTGT 
GACTGGGTGG CTACCATATT TTCTCGGGAT ATCCGATTTG CTAACCATGA GACATTACAG 
GTCATCTACC CCTATACCCC ACAAAATGAC GATGAGCTGG AGCTGGTCCC CGGGGACTTC 
ATCTTCATGT CTCCAATGGA GCAGACCAGC ACCAGCGAGG GTTGGATCTA TGGCACGTCC 
TTAACCACCG GCTGCTCTGG ACTCCTGCCT GAGAATTACA TTACCAAGGC TGATGAATGC 
AGCACCTGGA TATTTCATGG TTCTTATTCA ATCTTAAATA CATCGTCATC CAACTCTCTC 
ACGTTTGGGG ATGGAGTATT GGAGAGGCGG CCTTATGAGG ACCAGGGGCT CGGGGAGACG 
ACTCCTCTTA CTATCATCTG CCAGCCCATG CAGCCGCTGA GGGTCAACAG CCAGCCCGGC 
CCCCAGAAGC GATGCCTTTT TGTGTGTCGG CATGGTGAGA GGATGGATGT TGTGTTTGGG 
AAGTACTGGC TGTCCCAGTG CTTCGATGCC AAAGGCCGCT ACATACGCAC CAACCTGAAC 
ATGCCTCATA GTTTACCTCA GCGGAGTGGT GGTTTCCGAG ATTACGAGAA AGATGCTCCC 
ATCACTGTGT TTGGATGCAT GCAAGCAAGA CTAGTGGGTG AAGCCTTATT AGAGAGCAAT 
ACCATTATCG ATCATGTCTA TTGCTCCCCG TCCCTTCGCT GCGTTCAGAC TGCACACAAT 
ATCTTGAAAG GTTTACAACA AGAAAATCAC TTGAAGATCC GTGTAGAGCC CGGCTTATTT 
GAGTGGACAA AATGGGTTGC TGGGAGCACA TTACCTGCAT GGATACCTCC ATCAGAGTTA 
GCTGCAGCCA ACCTGAGTGT TGATACAACC TACAGACCTC ACATTCCAAT CAGCAAATTA 
GTTGTTTCAG AATCCTATGA TACTTATATC AGTAGAAGTT TCCAAGTAAC AAAAGAAATA 
ATAAGTGAAT GTAAAAGTAA AGGAAATAAC ATCCTGATTG TGGCCCACGC ATCTTCCCTT 
GAAGCGTGTA CCTGCCAACT TCAGGGCCTG TCACCTCAGA ACTCCAAGGA CTTCGTACAA 
ATGGTCCGAA AGATCCCATA TCTGGGATTT TGTTCCTGTG AAGAATTAGG AGAAACTGGA 
ATATGGCAGC TGACAGATCC ACCAATCCTT CCTCTTACCC ATGGACCAAC TGGGGGCTTC 
AACTGGAGAG AGACCTTGCT TCAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116262.2
CDS	329..2278
Translation	MAQYGHPSPLGMAAREELYSKVTPRRNRQQRPGTIKHGSALDVLLSMGFPRARAQKALASTGGRSVQAACDWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYTPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERRPYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTIIDHVYCSPSLRCVQTAHNILKGLQQENHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPISKLVVSESYDTYISRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE

Target ORF information:

RefSeq Version	NM_032873.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032873.5

ORF Insert Sequence:

ATGGCTCAGT ACGGCCACCC CAGTCCGCTC GGCATGGCTG CGAGAGAGGA GCTGTACAGC 
AAAGTCACCC CCCGGAGGAA CCGCCAACAG CGCCCCGGCA CCATCAAGCA TGGATCGGCG 
CTGGACGTGC TCCTCTCCAT GGGGTTCCCC AGAGCCCGCG CACAAAAAGC CTTGGCATCC 
ACGGGAGGAA GAAGTGTTCA GGCAGCATGT GACTGGTTAT TCTCCCATGT CGGTGACCCC 
TTCCTGGATG ACCCCCTGCC CCGGGAGTAC GTCCTCTACC TCCGTCCCAC CGGCCCCTTA 
GCACAGAAGC TTTCCGACTT TTGGCAGCAG TCGAAGCAGA TCTGCGGGAA GAACAAGGCA 
CACAACATCT TCCCCCACAT CACACTCTGC CAGTTCTTTA TGTGCGAGGA CAGCAAGGTG 
GATGCCCTGG GGGAAGCCCT GCAGACCACG GTCAGTCGCT GGAAATGTAA GTTCTCGGCC 
CCGCTGCCCC TGGAGCTCTA TACGTCGTCC AACTTCATCG GCCTCTTTGT AAAGGAAGAC 
AGTGCGGAGG TCCTCAAGAA GTTTGCTGCT GACTTTGCTG CAGAGGCTGC ATCCAAAACC 
GAAGTGCATG TGGAACCTCA TAAGAAGCAG CTACATGTGA CCCTGGCTTA CCACTTCCAA 
GCCAGCCACC TACCCACCCT AGAGAAACTG GCCCAGAACA TTGACGTCAA GCTAGGGTGT 
GACTGGGTGG CTACCATATT TTCTCGGGAT ATCCGATTTG CTAACCATGA GACATTACAG 
GTCATCTACC CCTATACCCC ACAAAATGAC GATGAGCTGG AGCTGGTCCC CGGGGACTTC 
ATCTTCATGT CTCCAATGGA GCAGACCAGC ACCAGCGAGG GTTGGATCTA TGGCACGTCC 
TTAACCACCG GCTGCTCTGG ACTCCTGCCT GAGAATTACA TTACCAAGGC TGATGAATGC 
AGCACCTGGA TATTTCATGG TTCTTATTCA ATCTTAAATA CATCGTCATC CAACTCTCTC 
ACGTTTGGGG ATGGAGTATT GGAGAGGCGG CCTTATGAGG ACCAGGGGCT CGGGGAGACG 
ACTCCTCTTA CTATCATCTG CCAGCCCATG CAGCCGCTGA GGGTCAACAG CCAGCCCGGC 
CCCCAGAAGC GATGCCTTTT TGTGTGTCGG CATGGTGAGA GGATGGATGT TGTGTTTGGG 
AAGTACTGGC TGTCCCAGTG CTTCGATGCC AAAGGCCGCT ACATACGCAC CAACCTGAAC 
ATGCCTCATA GTTTACCTCA GCGGAGTGGT GGTTTCCGAG ATTACGAGAA AGATGCTCCC 
ATCACTGTGT TTGGATGCAT GCAAGCAAGA CTAGTGGGTG AAGCCTTATT AGAGAGCAAT 
ACCATTATCG ATCATGTCTA TTGCTCCCCG TCCCTTCGCT GCGTTCAGAC TGCACACAAT 
ATCTTGAAAG GTTTACAACA AGAAAATCAC TTGAAGATCC GTGTAGAGCC CGGCTTATTT 
GAGTGGACAA AATGGGTTGC TGGGAGCACA TTACCTGCAT GGATACCTCC ATCAGAGTTA 
GCTGCAGCCA ACCTGAGTGT TGATACAACC TACAGACCTC ACATTCCAAT CAGCAAATTA 
GTTGTTTCAG AATCCTATGA TACTTATATC AGTAGAAGTT TCCAAGTAAC AAAAGAAATA 
ATAAGTGAAT GTAAAAGTAA AGGAAATAAC ATCCTGATTG TGGCCCACGC ATCTTCCCTT 
GAAGCGTGTA CCTGCCAACT TCAGGGCCTG TCACCTCAGA ACTCCAAGGA CTTCGTACAA 
ATGGTCCGAA AGATCCCATA TCTGGGATTT TGTTCCTGTG AAGAATTAGG AGAAACTGGA 
ATATGGCAGC TGACAGATCC ACCAATCCTT CCTCTTACCC ATGGACCAAC TGGGGGCTTC 
AACTGGAGAG AGACCTTGCT TCAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBASH3B ( NM_001363365.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350294.1 Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 2, mRNA.

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CloneID	OHu73896
Clone ID Related Accession (Same CDS sequence)	NM_001363365.1 , NM_001363365.2 , XM_011543042.1
Accession Version	NM_001363365.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-27
Organism	Homo sapiens(human)
Product	ubiquitin-associated and SH3 domain-containing protein B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP002762.4. On May 17, 2018 this sequence version replaced XM_011543042.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.102823.1, SRR1803611.157311.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGATCGGC GCTGGACGTG CTCCTCTCCA TGGGGTTCCC CAGAGCCCGC GCACAAAAAG 
CCTTGGCATC CACGGGAGGA AGAAGTGTTC AGGCAGCATG TGACTGGTTG GTTATTCTCC 
CATGTCGGTG ACCCCTTCCT GGATGACCCC CTGCCCCGGG AGTACGTCCT CTACCTCCGT 
CCCACCGGCC CCTTAGCACA GAAGCTTTCC GACTTTTGGC AGCAGTCGAA GCAGATCTGC 
GGGAAGAACA AGGCACACAA CATCTTCCCC CACATCACAC TCTGCCAGTT CTTTATGTGC 
GAGGACAGCA AGGTGGATGC CCTGGGGGAA GCCCTGCAGA CCACGGTCAG TCGCTGGAAA 
TGTAAGTTCT CGGCCCCGCT GCCCCTGGAG CTCTATACGT CGTCCAACTT CATCGGCCTC 
TTTGTAAAGG AAGACAGTGC GGAGGTCCTC AAGAAGTTTG CTGCTGACTT TGCTGCAGAG 
GCTGCATCCA AAACCGAAGT GCATGTGGAA CCTCATAAGA AGCAGCTACA TGTGACCCTG 
GCTTACCACT TCCAAGCCAG CCACCTACCC ACCCTAGAGA AACTGGCCCA GAACATTGAC 
GTCAAGCTAG GGTGTGACTG GGTGGCTACC ATATTTTCTC GGGATATCCG ATTTGCTAAC 
CATGAGACAT TACAGGTCAT CTACCCCTAT ACCCCACAAA ATGACGATGA GCTGGAGCTG 
GTCCCCGGGG ACTTCATCTT CATGTCTCCA ATGGAGCAGA CCAGCACCAG CGAGGGTTGG 
ATCTATGGCA CGTCCTTAAC CACCGGCTGC TCTGGACTCC TGCCTGAGAA TTACATTACC 
AAGGCTGATG AATGCAGCAC CTGGATATTT CATGGTTCTT ATTCAATCTT AAATACATCG 
TCATCCAACT CTCTCACGTT TGGGGATGGA GTATTGGAGA GGCGGCCTTA TGAGGACCAG 
GGGCTCGGGG AGACGACTCC TCTTACTATC ATCTGCCAGC CCATGCAGCC GCTGAGGGTC 
AACAGCCAGC CCGGCCCCCA GAAGCGATGC CTTTTTGTGT GTCGGCATGG TGAGAGGATG 
GATGTTGTGT TTGGGAAGTA CTGGCTGTCC CAGTGCTTCG ATGCCAAAGG CCGCTACATA 
CGCACCAACC TGAACATGCC TCATAGTTTA CCTCAGCGGA GTGGTGGTTT CCGAGATTAC 
GAGAAAGATG CTCCCATCAC TGTGTTTGGA TGCATGCAAG CAAGACTAGT GGGTGAAGCC 
TTATTAGAGA GCAATACCAT TATCGATCAT GTCTATTGCT CCCCGTCCCT TCGCTGCGTT 
CAGACTGCAC ACAATATCTT GAAAGGTTTA CAACAAGAAA ATCACTTGAA GATCCGTGTA 
GAGCCCGGCT TATTTGAGTG GACAAAATGG GTTGCTGGGA GCACATTACC TGCATGGATA 
CCTCCATCAG AGTTAGCTGC AGCCAACCTG AGTGTTGATA CAACCTACAG ACCTCACATT 
CCAATCAGCA AATTAGTTGT TTCAGAATCC TATGATACTT ATATCAGTAG AAGTTTCCAA 
GTAACAAAAG AAATAATAAG TGAATGTAAA AGTAAAGGAA ATAACATCCT GATTGTGGCC 
CACGCATCTT CCCTTGAAGC GTGTACCTGC CAACTTCAGG GCCTGTCACC TCAGAACTCC 
AAGGACTTCG TACAAATGGT CCGAAAGATC CCATATCTGG GATTTTGTTC CTGTGAAGAA 
TTAGGAGAAA CTGGAATATG GCAGCTGACA GATCCACCAA TCCTTCCTCT TACCCATGGA 
CCAACTGGGG GCTTCAACTG GAGAGAGACC TTGCTTCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350294.1
CDS	470..2314
Translation	MDRRWTCSSPWGSPEPAHKKPWHPREEEVFRQHVTGWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYTPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERRPYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTIIDHVYCSPSLRCVQTAHNILKGLQQENHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPISKLVVSESYDTYISRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE

Target ORF information:

RefSeq Version	NM_001363365.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363365.1

ORF Insert Sequence:

ATGGATCGGC GCTGGACGTG CTCCTCTCCA TGGGGTTCCC CAGAGCCCGC GCACAAAAAG 
CCTTGGCATC CACGGGAGGA AGAAGTGTTC AGGCAGCATG TGACTGGTTG GTTATTCTCC 
CATGTCGGTG ACCCCTTCCT GGATGACCCC CTGCCCCGGG AGTACGTCCT CTACCTCCGT 
CCCACCGGCC CCTTAGCACA GAAGCTTTCC GACTTTTGGC AGCAGTCGAA GCAGATCTGC 
GGGAAGAACA AGGCACACAA CATCTTCCCC CACATCACAC TCTGCCAGTT CTTTATGTGC 
GAGGACAGCA AGGTGGATGC CCTGGGGGAA GCCCTGCAGA CCACGGTCAG TCGCTGGAAA 
TGTAAGTTCT CGGCCCCGCT GCCCCTGGAG CTCTATACGT CGTCCAACTT CATCGGCCTC 
TTTGTAAAGG AAGACAGTGC GGAGGTCCTC AAGAAGTTTG CTGCTGACTT TGCTGCAGAG 
GCTGCATCCA AAACCGAAGT GCATGTGGAA CCTCATAAGA AGCAGCTACA TGTGACCCTG 
GCTTACCACT TCCAAGCCAG CCACCTACCC ACCCTAGAGA AACTGGCCCA GAACATTGAC 
GTCAAGCTAG GGTGTGACTG GGTGGCTACC ATATTTTCTC GGGATATCCG ATTTGCTAAC 
CATGAGACAT TACAGGTCAT CTACCCCTAT ACCCCACAAA ATGACGATGA GCTGGAGCTG 
GTCCCCGGGG ACTTCATCTT CATGTCTCCA ATGGAGCAGA CCAGCACCAG CGAGGGTTGG 
ATCTATGGCA CGTCCTTAAC CACCGGCTGC TCTGGACTCC TGCCTGAGAA TTACATTACC 
AAGGCTGATG AATGCAGCAC CTGGATATTT CATGGTTCTT ATTCAATCTT AAATACATCG 
TCATCCAACT CTCTCACGTT TGGGGATGGA GTATTGGAGA GGCGGCCTTA TGAGGACCAG 
GGGCTCGGGG AGACGACTCC TCTTACTATC ATCTGCCAGC CCATGCAGCC GCTGAGGGTC 
AACAGCCAGC CCGGCCCCCA GAAGCGATGC CTTTTTGTGT GTCGGCATGG TGAGAGGATG 
GATGTTGTGT TTGGGAAGTA CTGGCTGTCC CAGTGCTTCG ATGCCAAAGG CCGCTACATA 
CGCACCAACC TGAACATGCC TCATAGTTTA CCTCAGCGGA GTGGTGGTTT CCGAGATTAC 
GAGAAAGATG CTCCCATCAC TGTGTTTGGA TGCATGCAAG CAAGACTAGT GGGTGAAGCC 
TTATTAGAGA GCAATACCAT TATCGATCAT GTCTATTGCT CCCCGTCCCT TCGCTGCGTT 
CAGACTGCAC ACAATATCTT GAAAGGTTTA CAACAAGAAA ATCACTTGAA GATCCGTGTA 
GAGCCCGGCT TATTTGAGTG GACAAAATGG GTTGCTGGGA GCACATTACC TGCATGGATA 
CCTCCATCAG AGTTAGCTGC AGCCAACCTG AGTGTTGATA CAACCTACAG ACCTCACATT 
CCAATCAGCA AATTAGTTGT TTCAGAATCC TATGATACTT ATATCAGTAG AAGTTTCCAA 
GTAACAAAAG AAATAATAAG TGAATGTAAA AGTAAAGGAA ATAACATCCT GATTGTGGCC 
CACGCATCTT CCCTTGAAGC GTGTACCTGC CAACTTCAGG GCCTGTCACC TCAGAACTCC 
AAGGACTTCG TACAAATGGT CCGAAAGATC CCATATCTGG GATTTTGTTC CTGTGAAGAA 
TTAGGAGAAA CTGGAATATG GCAGCTGACA GATCCACCAA TCCTTCCTCT TACCCATGGA 
CCAACTGGGG GCTTCAACTG GAGAGAGACC TTGCTTCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBASH3B ( NM_001363365.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350294.1 Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 2, mRNA.

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CloneID	OHu73896
Clone ID Related Accession (Same CDS sequence)	NM_001363365.1 , NM_001363365.2 , XM_011543042.1
Accession Version	NM_001363365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	ubiquitin-associated and SH3 domain-containing protein B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP002762.4. On Jun 1, 2019 this sequence version replaced NM_001363365.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.157311.1, SRR1803613.102823.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGATCGGC GCTGGACGTG CTCCTCTCCA TGGGGTTCCC CAGAGCCCGC GCACAAAAAG 
CCTTGGCATC CACGGGAGGA AGAAGTGTTC AGGCAGCATG TGACTGGTTG GTTATTCTCC 
CATGTCGGTG ACCCCTTCCT GGATGACCCC CTGCCCCGGG AGTACGTCCT CTACCTCCGT 
CCCACCGGCC CCTTAGCACA GAAGCTTTCC GACTTTTGGC AGCAGTCGAA GCAGATCTGC 
GGGAAGAACA AGGCACACAA CATCTTCCCC CACATCACAC TCTGCCAGTT CTTTATGTGC 
GAGGACAGCA AGGTGGATGC CCTGGGGGAA GCCCTGCAGA CCACGGTCAG TCGCTGGAAA 
TGTAAGTTCT CGGCCCCGCT GCCCCTGGAG CTCTATACGT CGTCCAACTT CATCGGCCTC 
TTTGTAAAGG AAGACAGTGC GGAGGTCCTC AAGAAGTTTG CTGCTGACTT TGCTGCAGAG 
GCTGCATCCA AAACCGAAGT GCATGTGGAA CCTCATAAGA AGCAGCTACA TGTGACCCTG 
GCTTACCACT TCCAAGCCAG CCACCTACCC ACCCTAGAGA AACTGGCCCA GAACATTGAC 
GTCAAGCTAG GGTGTGACTG GGTGGCTACC ATATTTTCTC GGGATATCCG ATTTGCTAAC 
CATGAGACAT TACAGGTCAT CTACCCCTAT ACCCCACAAA ATGACGATGA GCTGGAGCTG 
GTCCCCGGGG ACTTCATCTT CATGTCTCCA ATGGAGCAGA CCAGCACCAG CGAGGGTTGG 
ATCTATGGCA CGTCCTTAAC CACCGGCTGC TCTGGACTCC TGCCTGAGAA TTACATTACC 
AAGGCTGATG AATGCAGCAC CTGGATATTT CATGGTTCTT ATTCAATCTT AAATACATCG 
TCATCCAACT CTCTCACGTT TGGGGATGGA GTATTGGAGA GGCGGCCTTA TGAGGACCAG 
GGGCTCGGGG AGACGACTCC TCTTACTATC ATCTGCCAGC CCATGCAGCC GCTGAGGGTC 
AACAGCCAGC CCGGCCCCCA GAAGCGATGC CTTTTTGTGT GTCGGCATGG TGAGAGGATG 
GATGTTGTGT TTGGGAAGTA CTGGCTGTCC CAGTGCTTCG ATGCCAAAGG CCGCTACATA 
CGCACCAACC TGAACATGCC TCATAGTTTA CCTCAGCGGA GTGGTGGTTT CCGAGATTAC 
GAGAAAGATG CTCCCATCAC TGTGTTTGGA TGCATGCAAG CAAGACTAGT GGGTGAAGCC 
TTATTAGAGA GCAATACCAT TATCGATCAT GTCTATTGCT CCCCGTCCCT TCGCTGCGTT 
CAGACTGCAC ACAATATCTT GAAAGGTTTA CAACAAGAAA ATCACTTGAA GATCCGTGTA 
GAGCCCGGCT TATTTGAGTG GACAAAATGG GTTGCTGGGA GCACATTACC TGCATGGATA 
CCTCCATCAG AGTTAGCTGC AGCCAACCTG AGTGTTGATA CAACCTACAG ACCTCACATT 
CCAATCAGCA AATTAGTTGT TTCAGAATCC TATGATACTT ATATCAGTAG AAGTTTCCAA 
GTAACAAAAG AAATAATAAG TGAATGTAAA AGTAAAGGAA ATAACATCCT GATTGTGGCC 
CACGCATCTT CCCTTGAAGC GTGTACCTGC CAACTTCAGG GCCTGTCACC TCAGAACTCC 
AAGGACTTCG TACAAATGGT CCGAAAGATC CCATATCTGG GATTTTGTTC CTGTGAAGAA 
TTAGGAGAAA CTGGAATATG GCAGCTGACA GATCCACCAA TCCTTCCTCT TACCCATGGA 
CCAACTGGGG GCTTCAACTG GAGAGAGACC TTGCTTCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350294.1
CDS	438..2282
Translation	MDRRWTCSSPWGSPEPAHKKPWHPREEEVFRQHVTGWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYTPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERRPYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTIIDHVYCSPSLRCVQTAHNILKGLQQENHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPISKLVVSESYDTYISRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE

Target ORF information:

RefSeq Version	NM_001363365.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363365.2

ORF Insert Sequence:

ATGGATCGGC GCTGGACGTG CTCCTCTCCA TGGGGTTCCC CAGAGCCCGC GCACAAAAAG 
CCTTGGCATC CACGGGAGGA AGAAGTGTTC AGGCAGCATG TGACTGGTTG GTTATTCTCC 
CATGTCGGTG ACCCCTTCCT GGATGACCCC CTGCCCCGGG AGTACGTCCT CTACCTCCGT 
CCCACCGGCC CCTTAGCACA GAAGCTTTCC GACTTTTGGC AGCAGTCGAA GCAGATCTGC 
GGGAAGAACA AGGCACACAA CATCTTCCCC CACATCACAC TCTGCCAGTT CTTTATGTGC 
GAGGACAGCA AGGTGGATGC CCTGGGGGAA GCCCTGCAGA CCACGGTCAG TCGCTGGAAA 
TGTAAGTTCT CGGCCCCGCT GCCCCTGGAG CTCTATACGT CGTCCAACTT CATCGGCCTC 
TTTGTAAAGG AAGACAGTGC GGAGGTCCTC AAGAAGTTTG CTGCTGACTT TGCTGCAGAG 
GCTGCATCCA AAACCGAAGT GCATGTGGAA CCTCATAAGA AGCAGCTACA TGTGACCCTG 
GCTTACCACT TCCAAGCCAG CCACCTACCC ACCCTAGAGA AACTGGCCCA GAACATTGAC 
GTCAAGCTAG GGTGTGACTG GGTGGCTACC ATATTTTCTC GGGATATCCG ATTTGCTAAC 
CATGAGACAT TACAGGTCAT CTACCCCTAT ACCCCACAAA ATGACGATGA GCTGGAGCTG 
GTCCCCGGGG ACTTCATCTT CATGTCTCCA ATGGAGCAGA CCAGCACCAG CGAGGGTTGG 
ATCTATGGCA CGTCCTTAAC CACCGGCTGC TCTGGACTCC TGCCTGAGAA TTACATTACC 
AAGGCTGATG AATGCAGCAC CTGGATATTT CATGGTTCTT ATTCAATCTT AAATACATCG 
TCATCCAACT CTCTCACGTT TGGGGATGGA GTATTGGAGA GGCGGCCTTA TGAGGACCAG 
GGGCTCGGGG AGACGACTCC TCTTACTATC ATCTGCCAGC CCATGCAGCC GCTGAGGGTC 
AACAGCCAGC CCGGCCCCCA GAAGCGATGC CTTTTTGTGT GTCGGCATGG TGAGAGGATG 
GATGTTGTGT TTGGGAAGTA CTGGCTGTCC CAGTGCTTCG ATGCCAAAGG CCGCTACATA 
CGCACCAACC TGAACATGCC TCATAGTTTA CCTCAGCGGA GTGGTGGTTT CCGAGATTAC 
GAGAAAGATG CTCCCATCAC TGTGTTTGGA TGCATGCAAG CAAGACTAGT GGGTGAAGCC 
TTATTAGAGA GCAATACCAT TATCGATCAT GTCTATTGCT CCCCGTCCCT TCGCTGCGTT 
CAGACTGCAC ACAATATCTT GAAAGGTTTA CAACAAGAAA ATCACTTGAA GATCCGTGTA 
GAGCCCGGCT TATTTGAGTG GACAAAATGG GTTGCTGGGA GCACATTACC TGCATGGATA 
CCTCCATCAG AGTTAGCTGC AGCCAACCTG AGTGTTGATA CAACCTACAG ACCTCACATT 
CCAATCAGCA AATTAGTTGT TTCAGAATCC TATGATACTT ATATCAGTAG AAGTTTCCAA 
GTAACAAAAG AAATAATAAG TGAATGTAAA AGTAAAGGAA ATAACATCCT GATTGTGGCC 
CACGCATCTT CCCTTGAAGC GTGTACCTGC CAACTTCAGG GCCTGTCACC TCAGAACTCC 
AAGGACTTCG TACAAATGGT CCGAAAGATC CCATATCTGG GATTTTGTTC CTGTGAAGAA 
TTAGGAGAAA CTGGAATATG GCAGCTGACA GATCCACCAA TCCTTCCTCT TACCCATGGA 
CCAACTGGGG GCTTCAACTG GAGAGAGACC TTGCTTCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBASH3B ( XM_011543041.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541343.1 Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu73895
Clone ID Related Accession (Same CDS sequence)	XM_011543041.2
Accession Version	XM_011543041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-associated and SH3 domain-containing protein B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGT GTGGGTCTCT GTGCTTCCAG CCTTTTCTGC ACCAGCTGTT CCAAGCGGAG 
CAGAGGGGTC TGTCTTGCTT CTCCGTAACT TCATCAACCT ACAAAAAGCC TTGGCATCCA 
CGGGAGGAAG AAGTGTTCAG GCAGCATGTG ACTGGTTGGT TATTCTCCCA TGTCGGTGAC 
CCCTTCCTGG ATGACCCCCT GCCCCGGGAG TACGTCCTCT ACCTCCGTCC CACCGGCCCC 
TTAGCACAGA AGCTTTCCGA CTTTTGGCAG CAGTCGAAGC AGATCTGCGG GAAGAACAAG 
GCACACAACA TCTTCCCCCA CATCACACTC TGCCAGTTCT TTATGTGCGA GGACAGCAAG 
GTGGATGCCC TGGGGGAAGC CCTGCAGACC ACGGTCAGTC GCTGGAAATG TAAGTTCTCG 
GCCCCGCTGC CCCTGGAGCT CTATACGTCG TCCAACTTCA TCGGCCTCTT TGTAAAGGAA 
GACAGTGCGG AGGTCCTCAA GAAGTTTGCT GCTGACTTTG CTGCAGAGGC TGCATCCAAA 
ACCGAAGTGC ATGTGGAACC TCATAAGAAG CAGCTACATG TGACCCTGGC TTACCACTTC 
CAAGCCAGCC ACCTACCCAC CCTAGAGAAA CTGGCCCAGA ACATTGACGT CAAGCTAGGG 
TGTGACTGGG TGGCTACCAT ATTTTCTCGG GATATCCGAT TTGCTAACCA TGAGACATTA 
CAGGTCATCT ACCCCTATAC CCCACAAAAT GACGATGAGC TGGAGCTGGT CCCCGGGGAC 
TTCATCTTCA TGTCTCCAAT GGAGCAGACC AGCACCAGCG AGGGTTGGAT CTATGGCACG 
TCCTTAACCA CCGGCTGCTC TGGACTCCTG CCTGAGAATT ACATTACCAA GGCTGATGAA 
TGCAGCACCT GGATATTTCA TGGTTCTTAT TCAATCTTAA ATACATCGTC ATCCAACTCT 
CTCACGTTTG GGGATGGAGT ATTGGAGAGG CGGCCTTATG AGGACCAGGG GCTCGGGGAG 
ACGACTCCTC TTACTATCAT CTGCCAGCCC ATGCAGCCGC TGAGGGTCAA CAGCCAGCCC 
GGCCCCCAGA AGCGATGCCT TTTTGTGTGT CGGCATGGTG AGAGGATGGA TGTTGTGTTT 
GGGAAGTACT GGCTGTCCCA GTGCTTCGAT GCCAAAGGCC GCTACATACG CACCAACCTG 
AACATGCCTC ATAGTTTACC TCAGCGGAGT GGTGGTTTCC GAGATTACGA GAAAGATGCT 
CCCATCACTG TGTTTGGATG CATGCAAGCA AGACTAGTGG GTGAAGCCTT ATTAGAGAGC 
AATACCATTA TCGATCATGT CTATTGCTCC CCGTCCCTTC GCTGCGTTCA GACTGCACAC 
AATATCTTGA AAGGTTTACA ACAAGAAAAT CACTTGAAGA TCCGTGTAGA GCCCGGCTTA 
TTTGAGTGGA CAAAATGGGT TGCTGGGAGC ACATTACCTG CATGGATACC TCCATCAGAG 
TTAGCTGCAG CCAACCTGAG TGTTGATACA ACCTACAGAC CTCACATTCC AATCAGCAAA 
TTAGTTGTTT CAGAATCCTA TGATACTTAT ATCAGTAGAA GTTTCCAAGT AACAAAAGAA 
ATAATAAGTG AATGTAAAAG TAAAGGAAAT AACATCCTGA TTGTGGCCCA CGCATCTTCC 
CTTGAAGCGT GTACCTGCCA ACTTCAGGGC CTGTCACCTC AGAACTCCAA GGACTTCGTA 
CAAATGGTCC GAAAGATCCC ATATCTGGGA TTTTGTTCCT GTGAAGAATT AGGAGAAACT 
GGAATATGGC AGCTGACAGA TCCACCAATC CTTCCTCTTA CCCATGGACC AACTGGGGGC 
TTCAACTGGA GAGAGACCTT GCTTCAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541343.1
CDS	56..1948
Translation	MSVCGSLCFQPFLHQLFQAEQRGLSCFSVTSSTYKKPWHPREEEVFRQHVTGWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYTPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERRPYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTIIDHVYCSPSLRCVQTAHNILKGLQQENHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPISKLVVSESYDTYISRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE

Target ORF information:

RefSeq Version	XM_011543041.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543041.2

ORF Insert Sequence:

ATGAGCGTGT GTGGGTCTCT GTGCTTCCAG CCTTTTCTGC ACCAGCTGTT CCAAGCGGAG 
CAGAGGGGTC TGTCTTGCTT CTCCGTAACT TCATCAACCT ACAAAAAGCC TTGGCATCCA 
CGGGAGGAAG AAGTGTTCAG GCAGCATGTG ACTGGTTGGT TATTCTCCCA TGTCGGTGAC 
CCCTTCCTGG ATGACCCCCT GCCCCGGGAG TACGTCCTCT ACCTCCGTCC CACCGGCCCC 
TTAGCACAGA AGCTTTCCGA CTTTTGGCAG CAGTCGAAGC AGATCTGCGG GAAGAACAAG 
GCACACAACA TCTTCCCCCA CATCACACTC TGCCAGTTCT TTATGTGCGA GGACAGCAAG 
GTGGATGCCC TGGGGGAAGC CCTGCAGACC ACGGTCAGTC GCTGGAAATG TAAGTTCTCG 
GCCCCGCTGC CCCTGGAGCT CTATACGTCG TCCAACTTCA TCGGCCTCTT TGTAAAGGAA 
GACAGTGCGG AGGTCCTCAA GAAGTTTGCT GCTGACTTTG CTGCAGAGGC TGCATCCAAA 
ACCGAAGTGC ATGTGGAACC TCATAAGAAG CAGCTACATG TGACCCTGGC TTACCACTTC 
CAAGCCAGCC ACCTACCCAC CCTAGAGAAA CTGGCCCAGA ACATTGACGT CAAGCTAGGG 
TGTGACTGGG TGGCTACCAT ATTTTCTCGG GATATCCGAT TTGCTAACCA TGAGACATTA 
CAGGTCATCT ACCCCTATAC CCCACAAAAT GACGATGAGC TGGAGCTGGT CCCCGGGGAC 
TTCATCTTCA TGTCTCCAAT GGAGCAGACC AGCACCAGCG AGGGTTGGAT CTATGGCACG 
TCCTTAACCA CCGGCTGCTC TGGACTCCTG CCTGAGAATT ACATTACCAA GGCTGATGAA 
TGCAGCACCT GGATATTTCA TGGTTCTTAT TCAATCTTAA ATACATCGTC ATCCAACTCT 
CTCACGTTTG GGGATGGAGT ATTGGAGAGG CGGCCTTATG AGGACCAGGG GCTCGGGGAG 
ACGACTCCTC TTACTATCAT CTGCCAGCCC ATGCAGCCGC TGAGGGTCAA CAGCCAGCCC 
GGCCCCCAGA AGCGATGCCT TTTTGTGTGT CGGCATGGTG AGAGGATGGA TGTTGTGTTT 
GGGAAGTACT GGCTGTCCCA GTGCTTCGAT GCCAAAGGCC GCTACATACG CACCAACCTG 
AACATGCCTC ATAGTTTACC TCAGCGGAGT GGTGGTTTCC GAGATTACGA GAAAGATGCT 
CCCATCACTG TGTTTGGATG CATGCAAGCA AGACTAGTGG GTGAAGCCTT ATTAGAGAGC 
AATACCATTA TCGATCATGT CTATTGCTCC CCGTCCCTTC GCTGCGTTCA GACTGCACAC 
AATATCTTGA AAGGTTTACA ACAAGAAAAT CACTTGAAGA TCCGTGTAGA GCCCGGCTTA 
TTTGAGTGGA CAAAATGGGT TGCTGGGAGC ACATTACCTG CATGGATACC TCCATCAGAG 
TTAGCTGCAG CCAACCTGAG TGTTGATACA ACCTACAGAC CTCACATTCC AATCAGCAAA 
TTAGTTGTTT CAGAATCCTA TGATACTTAT ATCAGTAGAA GTTTCCAAGT AACAAAAGAA 
ATAATAAGTG AATGTAAAAG TAAAGGAAAT AACATCCTGA TTGTGGCCCA CGCATCTTCC 
CTTGAAGCGT GTACCTGCCA ACTTCAGGGC CTGTCACCTC AGAACTCCAA GGACTTCGTA 
CAAATGGTCC GAAAGATCCC ATATCTGGGA TTTTGTTCCT GTGAAGAATT AGGAGAAACT 
GGAATATGGC AGCTGACAGA TCCACCAATC CTTCCTCTTA CCCATGGACC AACTGGGGGC 
TTCAACTGGA GAGAGACCTT GCTTCAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBASH3B ( XM_011543042.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541344.1 Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant X3, mRNA.

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CloneID	OHu73896
Clone ID Related Accession (Same CDS sequence)	NM_001363365.1 , NM_001363365.2 , XM_011543042.1
Accession Version	XM_011543042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin-associated and SH3 domain-containing protein B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGGC GCTGGACGTG CTCCTCTCCA TGGGGTTCCC CAGAGCCCGC GCACAAAAAG 
CCTTGGCATC CACGGGAGGA AGAAGTGTTC AGGCAGCATG TGACTGGTTG GTTATTCTCC 
CATGTCGGTG ACCCCTTCCT GGATGACCCC CTGCCCCGGG AGTACGTCCT CTACCTCCGT 
CCCACCGGCC CCTTAGCACA GAAGCTTTCC GACTTTTGGC AGCAGTCGAA GCAGATCTGC 
GGGAAGAACA AGGCACACAA CATCTTCCCC CACATCACAC TCTGCCAGTT CTTTATGTGC 
GAGGACAGCA AGGTGGATGC CCTGGGGGAA GCCCTGCAGA CCACGGTCAG TCGCTGGAAA 
TGTAAGTTCT CGGCCCCGCT GCCCCTGGAG CTCTATACGT CGTCCAACTT CATCGGCCTC 
TTTGTAAAGG AAGACAGTGC GGAGGTCCTC AAGAAGTTTG CTGCTGACTT TGCTGCAGAG 
GCTGCATCCA AAACCGAAGT GCATGTGGAA CCTCATAAGA AGCAGCTACA TGTGACCCTG 
GCTTACCACT TCCAAGCCAG CCACCTACCC ACCCTAGAGA AACTGGCCCA GAACATTGAC 
GTCAAGCTAG GGTGTGACTG GGTGGCTACC ATATTTTCTC GGGATATCCG ATTTGCTAAC 
CATGAGACAT TACAGGTCAT CTACCCCTAT ACCCCACAAA ATGACGATGA GCTGGAGCTG 
GTCCCCGGGG ACTTCATCTT CATGTCTCCA ATGGAGCAGA CCAGCACCAG CGAGGGTTGG 
ATCTATGGCA CGTCCTTAAC CACCGGCTGC TCTGGACTCC TGCCTGAGAA TTACATTACC 
AAGGCTGATG AATGCAGCAC CTGGATATTT CATGGTTCTT ATTCAATCTT AAATACATCG 
TCATCCAACT CTCTCACGTT TGGGGATGGA GTATTGGAGA GGCGGCCTTA TGAGGACCAG 
GGGCTCGGGG AGACGACTCC TCTTACTATC ATCTGCCAGC CCATGCAGCC GCTGAGGGTC 
AACAGCCAGC CCGGCCCCCA GAAGCGATGC CTTTTTGTGT GTCGGCATGG TGAGAGGATG 
GATGTTGTGT TTGGGAAGTA CTGGCTGTCC CAGTGCTTCG ATGCCAAAGG CCGCTACATA 
CGCACCAACC TGAACATGCC TCATAGTTTA CCTCAGCGGA GTGGTGGTTT CCGAGATTAC 
GAGAAAGATG CTCCCATCAC TGTGTTTGGA TGCATGCAAG CAAGACTAGT GGGTGAAGCC 
TTATTAGAGA GCAATACCAT TATCGATCAT GTCTATTGCT CCCCGTCCCT TCGCTGCGTT 
CAGACTGCAC ACAATATCTT GAAAGGTTTA CAACAAGAAA ATCACTTGAA GATCCGTGTA 
GAGCCCGGCT TATTTGAGTG GACAAAATGG GTTGCTGGGA GCACATTACC TGCATGGATA 
CCTCCATCAG AGTTAGCTGC AGCCAACCTG AGTGTTGATA CAACCTACAG ACCTCACATT 
CCAATCAGCA AATTAGTTGT TTCAGAATCC TATGATACTT ATATCAGTAG AAGTTTCCAA 
GTAACAAAAG AAATAATAAG TGAATGTAAA AGTAAAGGAA ATAACATCCT GATTGTGGCC 
CACGCATCTT CCCTTGAAGC GTGTACCTGC CAACTTCAGG GCCTGTCACC TCAGAACTCC 
AAGGACTTCG TACAAATGGT CCGAAAGATC CCATATCTGG GATTTTGTTC CTGTGAAGAA 
TTAGGAGAAA CTGGAATATG GCAGCTGACA GATCCACCAA TCCTTCCTCT TACCCATGGA 
CCAACTGGGG GCTTCAACTG GAGAGAGACC TTGCTTCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541344.1
CDS	470..2314
Translation	MDRRWTCSSPWGSPEPAHKKPWHPREEEVFRQHVTGWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYTPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERRPYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTIIDHVYCSPSLRCVQTAHNILKGLQQENHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPISKLVVSESYDTYISRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE

Target ORF information:

RefSeq Version	XM_011543042.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543042.1

ORF Insert Sequence:

ATGGATCGGC GCTGGACGTG CTCCTCTCCA TGGGGTTCCC CAGAGCCCGC GCACAAAAAG 
CCTTGGCATC CACGGGAGGA AGAAGTGTTC AGGCAGCATG TGACTGGTTG GTTATTCTCC 
CATGTCGGTG ACCCCTTCCT GGATGACCCC CTGCCCCGGG AGTACGTCCT CTACCTCCGT 
CCCACCGGCC CCTTAGCACA GAAGCTTTCC GACTTTTGGC AGCAGTCGAA GCAGATCTGC 
GGGAAGAACA AGGCACACAA CATCTTCCCC CACATCACAC TCTGCCAGTT CTTTATGTGC 
GAGGACAGCA AGGTGGATGC CCTGGGGGAA GCCCTGCAGA CCACGGTCAG TCGCTGGAAA 
TGTAAGTTCT CGGCCCCGCT GCCCCTGGAG CTCTATACGT CGTCCAACTT CATCGGCCTC 
TTTGTAAAGG AAGACAGTGC GGAGGTCCTC AAGAAGTTTG CTGCTGACTT TGCTGCAGAG 
GCTGCATCCA AAACCGAAGT GCATGTGGAA CCTCATAAGA AGCAGCTACA TGTGACCCTG 
GCTTACCACT TCCAAGCCAG CCACCTACCC ACCCTAGAGA AACTGGCCCA GAACATTGAC 
GTCAAGCTAG GGTGTGACTG GGTGGCTACC ATATTTTCTC GGGATATCCG ATTTGCTAAC 
CATGAGACAT TACAGGTCAT CTACCCCTAT ACCCCACAAA ATGACGATGA GCTGGAGCTG 
GTCCCCGGGG ACTTCATCTT CATGTCTCCA ATGGAGCAGA CCAGCACCAG CGAGGGTTGG 
ATCTATGGCA CGTCCTTAAC CACCGGCTGC TCTGGACTCC TGCCTGAGAA TTACATTACC 
AAGGCTGATG AATGCAGCAC CTGGATATTT CATGGTTCTT ATTCAATCTT AAATACATCG 
TCATCCAACT CTCTCACGTT TGGGGATGGA GTATTGGAGA GGCGGCCTTA TGAGGACCAG 
GGGCTCGGGG AGACGACTCC TCTTACTATC ATCTGCCAGC CCATGCAGCC GCTGAGGGTC 
AACAGCCAGC CCGGCCCCCA GAAGCGATGC CTTTTTGTGT GTCGGCATGG TGAGAGGATG 
GATGTTGTGT TTGGGAAGTA CTGGCTGTCC CAGTGCTTCG ATGCCAAAGG CCGCTACATA 
CGCACCAACC TGAACATGCC TCATAGTTTA CCTCAGCGGA GTGGTGGTTT CCGAGATTAC 
GAGAAAGATG CTCCCATCAC TGTGTTTGGA TGCATGCAAG CAAGACTAGT GGGTGAAGCC 
TTATTAGAGA GCAATACCAT TATCGATCAT GTCTATTGCT CCCCGTCCCT TCGCTGCGTT 
CAGACTGCAC ACAATATCTT GAAAGGTTTA CAACAAGAAA ATCACTTGAA GATCCGTGTA 
GAGCCCGGCT TATTTGAGTG GACAAAATGG GTTGCTGGGA GCACATTACC TGCATGGATA 
CCTCCATCAG AGTTAGCTGC AGCCAACCTG AGTGTTGATA CAACCTACAG ACCTCACATT 
CCAATCAGCA AATTAGTTGT TTCAGAATCC TATGATACTT ATATCAGTAG AAGTTTCCAA 
GTAACAAAAG AAATAATAAG TGAATGTAAA AGTAAAGGAA ATAACATCCT GATTGTGGCC 
CACGCATCTT CCCTTGAAGC GTGTACCTGC CAACTTCAGG GCCTGTCACC TCAGAACTCC 
AAGGACTTCG TACAAATGGT CCGAAAGATC CCATATCTGG GATTTTGTTC CTGTGAAGAA 
TTAGGAGAAA CTGGAATATG GCAGCTGACA GATCCACCAA TCCTTCCTCT TACCCATGGA 
CCAACTGGGG GCTTCAACTG GAGAGAGACC TTGCTTCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBASH3B ( XM_005271712.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271769.1 Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant X1, mRNA.

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CloneID	OHu45904
Clone ID Related Accession (Same CDS sequence)	XM_005271712.3
Accession Version	XM_005271712.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-associated and SH3 domain-containing protein B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005271712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTGTG TTGTTTTGTG CATACACGGC TTCTGTACTG AAAAAAAAAA AGCGAGATGT 
GTGGCCTCTG ATTGTCTAGT AAGTCGTGAG GAAACAGACA AGGCTGCAAC CCAGCAGCAG 
TTGAGCACCT TGTCAAAGTG TGGGAATGAG CGTGTGTGGG TCTCTGTGCT TCCAGCCTTT 
TCTGCACCAG CTGTTCCAAG CGGAGCAGAG GGGTCTGTCT TGCTTCTCCG TAACTTCATC 
AACCTACAAA AAGCCTTGGC ATCCACGGGA GGAAGAAGTG TTCAGGCAGC ATGTGACTGG 
TTATTCTCCC ATGTCGGTGA CCCCTTCCTG GATGACCCCC TGCCCCGGGA GTACGTCCTC 
TACCTCCGTC CCACCGGCCC CTTAGCACAG AAGCTTTCCG ACTTTTGGCA GCAGTCGAAG 
CAGATCTGCG GGAAGAACAA GGCACACAAC ATCTTCCCCC ACATCACACT CTGCCAGTTC 
TTTATGTGCG AGGACAGCAA GGTGGATGCC CTGGGGGAAG CCCTGCAGAC CACGGTCAGT 
CGCTGGAAAT GTAAGTTCTC GGCCCCGCTG CCCCTGGAGC TCTATACGTC GTCCAACTTC 
ATCGGCCTCT TTGTAAAGGA AGACAGTGCG GAGGTCCTCA AGAAGTTTGC TGCTGACTTT 
GCTGCAGAGG CTGCATCCAA AACCGAAGTG CATGTGGAAC CTCATAAGAA GCAGCTACAT 
GTGACCCTGG CTTACCACTT CCAAGCCAGC CACCTACCCA CCCTAGAGAA ACTGGCCCAG 
AACATTGACG TCAAGCTAGG GTGTGACTGG GTGGCTACCA TATTTTCTCG GGATATCCGA 
TTTGCTAACC ATGAGACATT ACAGGTCATC TACCCCTATA CCCCACAAAA TGACGATGAG 
CTGGAGCTGG TCCCCGGGGA CTTCATCTTC ATGTCTCCAA TGGAGCAGAC CAGCACCAGC 
GAGGGTTGGA TCTATGGCAC GTCCTTAACC ACCGGCTGCT CTGGACTCCT GCCTGAGAAT 
TACATTACCA AGGCTGATGA ATGCAGCACC TGGATATTTC ATGGTTCTTA TTCAATCTTA 
AATACATCGT CATCCAACTC TCTCACGTTT GGGGATGGAG TATTGGAGAG GCGGCCTTAT 
GAGGACCAGG GGCTCGGGGA GACGACTCCT CTTACTATCA TCTGCCAGCC CATGCAGCCG 
CTGAGGGTCA ACAGCCAGCC CGGCCCCCAG AAGCGATGCC TTTTTGTGTG TCGGCATGGT 
GAGAGGATGG ATGTTGTGTT TGGGAAGTAC TGGCTGTCCC AGTGCTTCGA TGCCAAAGGC 
CGCTACATAC GCACCAACCT GAACATGCCT CATAGTTTAC CTCAGCGGAG TGGTGGTTTC 
CGAGATTACG AGAAAGATGC TCCCATCACT GTGTTTGGAT GCATGCAAGC AAGACTAGTG 
GGTGAAGCCT TATTAGAGAG CAATACCATT ATCGATCATG TCTATTGCTC CCCGTCCCTT 
CGCTGCGTTC AGACTGCACA CAATATCTTG AAAGGTTTAC AACAAGAAAA TCACTTGAAG 
ATCCGTGTAG AGCCCGGCTT ATTTGAGTGG ACAAAATGGG TTGCTGGGAG CACATTACCT 
GCATGGATAC CTCCATCAGA GTTAGCTGCA GCCAACCTGA GTGTTGATAC AACCTACAGA 
CCTCACATTC CAATCAGCAA ATTAGTTGTT TCAGAATCCT ATGATACTTA TATCAGTAGA 
AGTTTCCAAG TAACAAAAGA AATAATAAGT GAATGTAAAA GTAAAGGAAA TAACATCCTG 
ATTGTGGCCC ACGCATCTTC CCTTGAAGCG TGTACCTGCC AACTTCAGGG CCTGTCACCT 
CAGAACTCCA AGGACTTCGT ACAAATGGTC CGAAAGATCC CATATCTGGG ATTTTGTTCC 
TGTGAAGAAT TAGGAGAAAC TGGAATATGG CAGCTGACAG ATCCACCAAT CCTTCCTCTT 
ACCCATGGAC CAACTGGGGG CTTCAACTGG AGAGAGACCT TGCTTCAAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271769.1
CDS	2523..4556
Translation	MICVVLCIHGFCTEKKKARCVASDCLVSREETDKAATQQQLSTLSKCGNERVWVSVLPAFSAPAVPSGAEGSVLLLRNFINLQKALASTGGRSVQAACDWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYTPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERRPYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTIIDHVYCSPSLRCVQTAHNILKGLQQENHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPISKLVVSESYDTYISRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE

Target ORF information:

RefSeq Version	XM_005271712.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005271712.3

ORF Insert Sequence:

ATGATTTGTG TTGTTTTGTG CATACACGGC TTCTGTACTG AAAAAAAAAA AGCGAGATGT 
GTGGCCTCTG ATTGTCTAGT AAGTCGTGAG GAAACAGACA AGGCTGCAAC CCAGCAGCAG 
TTGAGCACCT TGTCAAAGTG TGGGAATGAG CGTGTGTGGG TCTCTGTGCT TCCAGCCTTT 
TCTGCACCAG CTGTTCCAAG CGGAGCAGAG GGGTCTGTCT TGCTTCTCCG TAACTTCATC 
AACCTACAAA AAGCCTTGGC ATCCACGGGA GGAAGAAGTG TTCAGGCAGC ATGTGACTGG 
TTATTCTCCC ATGTCGGTGA CCCCTTCCTG GATGACCCCC TGCCCCGGGA GTACGTCCTC 
TACCTCCGTC CCACCGGCCC CTTAGCACAG AAGCTTTCCG ACTTTTGGCA GCAGTCGAAG 
CAGATCTGCG GGAAGAACAA GGCACACAAC ATCTTCCCCC ACATCACACT CTGCCAGTTC 
TTTATGTGCG AGGACAGCAA GGTGGATGCC CTGGGGGAAG CCCTGCAGAC CACGGTCAGT 
CGCTGGAAAT GTAAGTTCTC GGCCCCGCTG CCCCTGGAGC TCTATACGTC GTCCAACTTC 
ATCGGCCTCT TTGTAAAGGA AGACAGTGCG GAGGTCCTCA AGAAGTTTGC TGCTGACTTT 
GCTGCAGAGG CTGCATCCAA AACCGAAGTG CATGTGGAAC CTCATAAGAA GCAGCTACAT 
GTGACCCTGG CTTACCACTT CCAAGCCAGC CACCTACCCA CCCTAGAGAA ACTGGCCCAG 
AACATTGACG TCAAGCTAGG GTGTGACTGG GTGGCTACCA TATTTTCTCG GGATATCCGA 
TTTGCTAACC ATGAGACATT ACAGGTCATC TACCCCTATA CCCCACAAAA TGACGATGAG 
CTGGAGCTGG TCCCCGGGGA CTTCATCTTC ATGTCTCCAA TGGAGCAGAC CAGCACCAGC 
GAGGGTTGGA TCTATGGCAC GTCCTTAACC ACCGGCTGCT CTGGACTCCT GCCTGAGAAT 
TACATTACCA AGGCTGATGA ATGCAGCACC TGGATATTTC ATGGTTCTTA TTCAATCTTA 
AATACATCGT CATCCAACTC TCTCACGTTT GGGGATGGAG TATTGGAGAG GCGGCCTTAT 
GAGGACCAGG GGCTCGGGGA GACGACTCCT CTTACTATCA TCTGCCAGCC CATGCAGCCG 
CTGAGGGTCA ACAGCCAGCC CGGCCCCCAG AAGCGATGCC TTTTTGTGTG TCGGCATGGT 
GAGAGGATGG ATGTTGTGTT TGGGAAGTAC TGGCTGTCCC AGTGCTTCGA TGCCAAAGGC 
CGCTACATAC GCACCAACCT GAACATGCCT CATAGTTTAC CTCAGCGGAG TGGTGGTTTC 
CGAGATTACG AGAAAGATGC TCCCATCACT GTGTTTGGAT GCATGCAAGC AAGACTAGTG 
GGTGAAGCCT TATTAGAGAG CAATACCATT ATCGATCATG TCTATTGCTC CCCGTCCCTT 
CGCTGCGTTC AGACTGCACA CAATATCTTG AAAGGTTTAC AACAAGAAAA TCACTTGAAG 
ATCCGTGTAG AGCCCGGCTT ATTTGAGTGG ACAAAATGGG TTGCTGGGAG CACATTACCT 
GCATGGATAC CTCCATCAGA GTTAGCTGCA GCCAACCTGA GTGTTGATAC AACCTACAGA 
CCTCACATTC CAATCAGCAA ATTAGTTGTT TCAGAATCCT ATGATACTTA TATCAGTAGA 
AGTTTCCAAG TAACAAAAGA AATAATAAGT GAATGTAAAA GTAAAGGAAA TAACATCCTG 
ATTGTGGCCC ACGCATCTTC CCTTGAAGCG TGTACCTGCC AACTTCAGGG CCTGTCACCT 
CAGAACTCCA AGGACTTCGT ACAAATGGTC CGAAAGATCC CATATCTGGG ATTTTGTTCC 
TGTGAAGAAT TAGGAGAAAC TGGAATATGG CAGCTGACAG ATCCACCAAT CCTTCCTCTT 
ACCCATGGAC CAACTGGGGG CTTCAACTGG AGAGAGACCT TGCTTCAAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of STS-1 as a novel ShcA-binding protein.
Biochemical and biophysical research communications490(4)1334-1339(2017 09)
van der Meulen T,Swarts S,Fischer W,van der Geer P

UBASH3B/Sts-1-CBL axis regulates myeloid proliferation in human preleukemia induced by AML1-ETO.
Leukemia30(3)728-39(2016 Mar)
Goyama S,Schibler J,Gasilina A,Shrestha M,Lin S,Link KA,Chen J,Whitman SP,Bloomfield CD,Nicolet D,Assi SA,Ptasinska A,Heidenreich O,Bonifer C,Kitamura T,Nassar NN,Mulloy JC

Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules.
Developmental cell36(1)63-78(2016 Jan)
Krupina K,Kleiss C,Metzger T,Fournane S,Schmucker S,Hofmann K,Fischer B,Paul N,Porter IM,Raffelsberger W,Poch O,Swedlow JR,Brino L,Sumara I

Anti-miR-148a regulates platelet Fc?RIIA signaling and decreases thrombosis in vivo in mice.
Blood126(26)2871-81(2015 Dec)
Zhou Y,Abraham S,Andre P,Edelstein LC,Shaw CA,Dangelmaier CA,Tsygankov AY,Kunapuli SP,Bray PF,McKenzie SE

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UBASH3B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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