PRPF38A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRPF38A
Entrez Gene ID	84950
Full Name	pre-mRNA processing factor 38A
Synonyms	PRP38A,Prp38
General protein information	Preferred Names pre-mRNA processing factor 38A Names pre-mRNA-splicing factor 38A PRP38 pre-mRNA processing factor 38 domain containing A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p32.3
Disorder MIM:	617031

mRNA and Protein(s)

mRNA	Protein	Name
XM_011542315.2	XP_011540617.1	pre-mRNA-splicing factor 38A isoform X1
NM_032864.4	NP_116253.2	pre-mRNA-splicing factor 38A
XM_011542315.3	XP_011540617.1	pre-mRNA-splicing factor 38A isoform X1
NM_032864.3	NP_116253.2	pre-mRNA-splicing factor 38A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PRPF38A	XP_532574.2
Mus musculus (house mouse)	Prpf38a	NP_766285.2
Danio rerio (zebrafish)	prpf38a	NP_001003483.1
Homo sapiens (human)	PRPF38A	NP_116253.2
Pan troglodytes (chimpanzee)	PRPF38A	XP_513409.2
	LOC713344	XP_001111695.1
Gallus gallus (chicken)	PRPF38A	NP_001264596.1
Bos taurus (cattle)	PRPF38A	NP_001068775.1
Schizosaccharomyces pombe (fission yeast)	prp38	NP_595693.1
Xenopus tropicalis (tropical clawed frog)	prpf38a	NP_001017140.1
Rattus norvegicus (Norway rat)	Prpf38a	NP_001100142.1
Magnaporthe oryzae (rice blast fungus)	MGG_03636	XP_003716253.1
Neurospora crassa	NCU00662	XP_965802.2

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, L?hrmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

Multiple protein-protein interactions converging on the Prp38 protein during activation of the human spliceosome.
Sch?tze T, Ulrich AK, Apelt L, Will CL, Bartlick N, Seeger M, Weber G, L?hrmann R, Stelzl U, Wahl MC
RNA (New York, N.Y.)22(2)265-77(2016 Feb)

A Y2H-seq approach defines the human protein methyltransferase interactome.
Weimann M, Grossmann A, Woodsmith J, ?zkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U
Nature methods10(4)339-42(2013 Apr)

The protein interaction landscape of the human CMGC kinase group.
Varjosalo M, Keskitalo S, Van Drogen A, Nurkkala H, Vichalkovski A, Aebersold R, Gstaiger M
Cell reports3(4)1306-20(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Multiple protein-protein interactions are converging on the Prp38 protein during activation of the human spliceosome.
Title: Multiple protein-protein interactions converging on the Prp38 protein during activation of the human spliceosome.

The following PRPF38A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPF38A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13528	NM_032864.4 Latest version!	Homo sapiens pre-mRNA processing factor 38A (PRPF38A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu73888	XM_011542315.3 Latest version!	Homo sapiens pre-mRNA processing factor 38A (PRPF38A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu73888	XM_011542315.2	Homo sapiens pre-mRNA processing factor 38A (PRPF38A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu13528	NM_032864.3	Homo sapiens pre-mRNA processing factor 38A (PRPF38A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRPF38A ( NM_032864.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_116253.2 Homo sapiens pre-mRNA processing factor 38A (PRPF38A), mRNA.

Price & Availability↑

CloneID	OHu13528
Clone ID Related Accession (Same CDS sequence)	NM_032864.4 , NM_032864.3
Accession Version	NM_032864.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor 38A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK092038.1, BU508261.1, BF223635.1, BE465896.1 and AL138849.12. On Nov 22, 2018 this sequence version replaced NM_032864.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.141408.1, SRR1660803.23158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000257181.10/ ENSP00000257181.8 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTAACC GTACAGTGAA GGATGCGCAC AGCATCCATG GCACCAACCC TCAATATCTG 
GTGGAGAAGA TCATTCGAAC GCGAATCTAT GAGTCCAAGT ACTGGAAAGA GGAGTGCTTT 
GGACTTACAG CTGAACTTGT AGTCGATAAA GCCATGGAGT TAAGGTTTGT GGGTGGCGTC 
TATGGTGGCA ACATAAAACC AACACCCTTT CTGTGTTTAA CCTTGAAGAT GCTTCAAATT 
CAACCCGAGA AGGATATCAT TGTAGAGTTT ATCAAAAATG AAGATTTCAA GTATGTCCGC 
ATGCTGGGGG CACTTTACAT GAGGCTGACA GGCACTGCAA TTGATTGCTA CAAGTACTTG 
GAACCTTTGT ACAATGACTA TCGAAAAATC AAGAGCCAGA ACCGAAATGG GGAGTTTGAA 
TTGATGCATG TTGATGAGTT TATTGATGAA CTATTGCACA GTGAGAGAGT CTGTGATATC 
ATTCTGCCCC GACTACAGAA ACGCTATGTA TTAGAGGAAG CTGAGCAACT GGAGCCTCGA 
GTTAGTGCTC TGGAAGAGGA CATGGATGAT GTGGAGTCCA GTGAAGAGGA AGAAGAGGAG 
GATGAGAAGT TGGAAAGAGT GCCATCACCT GATCACCGCC GGAGAAGCTA CCGAGACTTG 
GACAAGCCCC GTCGCTCTCC CACACTGCGC TACAGGAGGA GTAGGAGCCG GTCTCCCAGA 
AGGCGGAGTC GATCTCCCAA AAGGAGAAGC CCCTCCCCTC GCCGAGAAAG GCATCGGAGC 
AAGAGTCCAA GACGTCACCG CAGCAGGTCC CGAGATCGGC GGCACAGATC CCGTTCCAAG 
TCCCCAGGTC ATCACCGTAG TCACAGACAC AGGAGCCACT CAAAGTCTCC CGAAAGGTCT 
AAGAAGAGCC ACAAGAAGAG CCGGAGAGGG AATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116253.2
CDS	149..1087
Translation	MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTAELVVDKAMELRFVGGVYGGNIKPTPFLCLTLKMLQIQPEKDIIVEFIKNEDFKYVRMLGALYMRLTGTAIDCYKYLEPLYNDYRKIKSQNRNGEFELMHVDEFIDELLHSERVCDIILPRLQKRYVLEEAEQLEPRVSALEEDMDDVESSEEEEEEDEKLERVPSPDHRRRSYRDLDKPRRSPTLRYRRSRSRSPRRRSRSPKRRSPSPRRERHRSKSPRRHRSRSRDRRHRSRSKSPGHHRSHRHRSHSKSPERSKKSHKKSRRGNE

Target ORF information:

RefSeq Version	NM_032864.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pre-mRNA processing factor 38A (PRPF38A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032864.4

ORF Insert Sequence:

ATGGCTAACC GTACAGTGAA GGATGCGCAC AGCATCCATG GCACCAACCC TCAATATCTG 
GTGGAGAAGA TCATTCGAAC GCGAATCTAT GAGTCCAAGT ACTGGAAAGA GGAGTGCTTT 
GGACTTACAG CTGAACTTGT AGTCGATAAA GCCATGGAGT TAAGGTTTGT GGGTGGCGTC 
TATGGTGGCA ACATAAAACC AACACCCTTT CTGTGTTTAA CCTTGAAGAT GCTTCAAATT 
CAACCCGAGA AGGATATCAT TGTAGAGTTT ATCAAAAATG AAGATTTCAA GTATGTCCGC 
ATGCTGGGGG CACTTTACAT GAGGCTGACA GGCACTGCAA TTGATTGCTA CAAGTACTTG 
GAACCTTTGT ACAATGACTA TCGAAAAATC AAGAGCCAGA ACCGAAATGG GGAGTTTGAA 
TTGATGCATG TTGATGAGTT TATTGATGAA CTATTGCACA GTGAGAGAGT CTGTGATATC 
ATTCTGCCCC GACTACAGAA ACGCTATGTA TTAGAGGAAG CTGAGCAACT GGAGCCTCGA 
GTTAGTGCTC TGGAAGAGGA CATGGATGAT GTGGAGTCCA GTGAAGAGGA AGAAGAGGAG 
GATGAGAAGT TGGAAAGAGT GCCATCACCT GATCACCGCC GGAGAAGCTA CCGAGACTTG 
GACAAGCCCC GTCGCTCTCC CACACTGCGC TACAGGAGGA GTAGGAGCCG GTCTCCCAGA 
AGGCGGAGTC GATCTCCCAA AAGGAGAAGC CCCTCCCCTC GCCGAGAAAG GCATCGGAGC 
AAGAGTCCAA GACGTCACCG CAGCAGGTCC CGAGATCGGC GGCACAGATC CCGTTCCAAG 
TCCCCAGGTC ATCACCGTAG TCACAGACAC AGGAGCCACT CAAAGTCTCC CGAAAGGTCT 
AAGAAGAGCC ACAAGAAGAG CCGGAGAGGG AATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF38A ( NM_032864.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116253.2 Homo sapiens pre-mRNA processing factor 38A (PRPF38A), mRNA.

Price & Availability↑

CloneID	OHu13528
Clone ID Related Accession (Same CDS sequence)	NM_032864.4 , NM_032864.3
Accession Version	NM_032864.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor 38A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA055001.1, AK092038.1, BU508261.1, BF223635.1 and BE465896.1. On Jun 21, 2007 this sequence version replaced NM_032864.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.57346.1, SRR1660809.26471.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTAACC GTACAGTGAA GGATGCGCAC AGCATCCATG GCACCAACCC TCAATATCTG 
GTGGAGAAGA TCATTCGAAC GCGAATCTAT GAGTCCAAGT ACTGGAAAGA GGAGTGCTTT 
GGACTTACAG CTGAACTTGT AGTCGATAAA GCCATGGAGT TAAGGTTTGT GGGTGGCGTC 
TATGGTGGCA ACATAAAACC AACACCCTTT CTGTGTTTAA CCTTGAAGAT GCTTCAAATT 
CAACCCGAGA AGGATATCAT TGTAGAGTTT ATCAAAAATG AAGATTTCAA GTATGTCCGC 
ATGCTGGGGG CACTTTACAT GAGGCTGACA GGCACTGCAA TTGATTGCTA CAAGTACTTG 
GAACCTTTGT ACAATGACTA TCGAAAAATC AAGAGCCAGA ACCGAAATGG GGAGTTTGAA 
TTGATGCATG TTGATGAGTT TATTGATGAA CTATTGCACA GTGAGAGAGT CTGTGATATC 
ATTCTGCCCC GACTACAGAA ACGCTATGTA TTAGAGGAAG CTGAGCAACT GGAGCCTCGA 
GTTAGTGCTC TGGAAGAGGA CATGGATGAT GTGGAGTCCA GTGAAGAGGA AGAAGAGGAG 
GATGAGAAGT TGGAAAGAGT GCCATCACCT GATCACCGCC GGAGAAGCTA CCGAGACTTG 
GACAAGCCCC GTCGCTCTCC CACACTGCGC TACAGGAGGA GTAGGAGCCG GTCTCCCAGA 
AGGCGGAGTC GATCTCCCAA AAGGAGAAGC CCCTCCCCTC GCCGAGAAAG GCATCGGAGC 
AAGAGTCCAA GACGTCACCG CAGCAGGTCC CGAGATCGGC GGCACAGATC CCGTTCCAAG 
TCCCCAGGTC ATCACCGTAG TCACAGACAC AGGAGCCACT CAAAGTCTCC CGAAAGGTCT 
AAGAAGAGCC ACAAGAAGAG CCGGAGAGGG AATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116253.2
CDS	204..1142
Translation	MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTAELVVDKAMELRFVGGVYGGNIKPTPFLCLTLKMLQIQPEKDIIVEFIKNEDFKYVRMLGALYMRLTGTAIDCYKYLEPLYNDYRKIKSQNRNGEFELMHVDEFIDELLHSERVCDIILPRLQKRYVLEEAEQLEPRVSALEEDMDDVESSEEEEEEDEKLERVPSPDHRRRSYRDLDKPRRSPTLRYRRSRSRSPRRRSRSPKRRSPSPRRERHRSKSPRRHRSRSRDRRHRSRSKSPGHHRSHRHRSHSKSPERSKKSHKKSRRGNE

Target ORF information:

RefSeq Version	NM_032864.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pre-mRNA processing factor 38A (PRPF38A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032864.3

ORF Insert Sequence:

ATGGCTAACC GTACAGTGAA GGATGCGCAC AGCATCCATG GCACCAACCC TCAATATCTG 
GTGGAGAAGA TCATTCGAAC GCGAATCTAT GAGTCCAAGT ACTGGAAAGA GGAGTGCTTT 
GGACTTACAG CTGAACTTGT AGTCGATAAA GCCATGGAGT TAAGGTTTGT GGGTGGCGTC 
TATGGTGGCA ACATAAAACC AACACCCTTT CTGTGTTTAA CCTTGAAGAT GCTTCAAATT 
CAACCCGAGA AGGATATCAT TGTAGAGTTT ATCAAAAATG AAGATTTCAA GTATGTCCGC 
ATGCTGGGGG CACTTTACAT GAGGCTGACA GGCACTGCAA TTGATTGCTA CAAGTACTTG 
GAACCTTTGT ACAATGACTA TCGAAAAATC AAGAGCCAGA ACCGAAATGG GGAGTTTGAA 
TTGATGCATG TTGATGAGTT TATTGATGAA CTATTGCACA GTGAGAGAGT CTGTGATATC 
ATTCTGCCCC GACTACAGAA ACGCTATGTA TTAGAGGAAG CTGAGCAACT GGAGCCTCGA 
GTTAGTGCTC TGGAAGAGGA CATGGATGAT GTGGAGTCCA GTGAAGAGGA AGAAGAGGAG 
GATGAGAAGT TGGAAAGAGT GCCATCACCT GATCACCGCC GGAGAAGCTA CCGAGACTTG 
GACAAGCCCC GTCGCTCTCC CACACTGCGC TACAGGAGGA GTAGGAGCCG GTCTCCCAGA 
AGGCGGAGTC GATCTCCCAA AAGGAGAAGC CCCTCCCCTC GCCGAGAAAG GCATCGGAGC 
AAGAGTCCAA GACGTCACCG CAGCAGGTCC CGAGATCGGC GGCACAGATC CCGTTCCAAG 
TCCCCAGGTC ATCACCGTAG TCACAGACAC AGGAGCCACT CAAAGTCTCC CGAAAGGTCT 
AAGAAGAGCC ACAAGAAGAG CCGGAGAGGG AATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF38A ( XM_011542315.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540617.1 Homo sapiens pre-mRNA processing factor 38A (PRPF38A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu73888
Clone ID Related Accession (Same CDS sequence)	XM_011542315.2 , XM_011542315.3
Accession Version	XM_011542315.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor 38A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542315.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACC GTACAGTGAA GGATGCGCAC AGCATCCATG GCACCAACCC TCAATATCTG 
GTGGAGAAGA TCATTCGAAC GCGAATCTAT GAGTCCAAGT ACTGGAAAGA GGAGTGCTTT 
GGACTTACAG CTGAACTTGT AGTCGATAAA GCCATGGAGT TAAGGTTTGT GGGTGGCGTC 
TATGGTGGCA ACATAAAACC AACACCCTTT CTGTGTTTAA CCTTGAAGAT GCTTCAAATT 
CAACCCGAGA AGGATATCAT TGTAGAGTTT ATCAAAAATG AAGATTTCAA GTATGTCCGC 
ATGCTGGGGG CACTTTACAT GAGGCTGACA GGCACTGCAA TTGATTGCTA CAAGTACTTG 
GAACCTTTGT ACAATGACTA TCGAAAAATC AAGAGCCAGA ACCGAAATGG GGAGTTTGAA 
TTGATGCATG TTGATGAGTT TATTGATGAA CTATTGCACA GTGAGAGAGT CTGTGATATC 
ATTCTGCCCC GACTACAGAA ACGCTATGTA TTAGAGGAAG CTGAGCAACT GGAGCCTCGA 
GTTAGTGCTC TGGAAGAGGA CATGGATGAT GTGGAGTCCA GTGAAGAGGA AGAAGAGGAG 
GATGAGAAGT TGGAAAGAGT GCCATCACCT GATCACCGCC GGAGAAGCTA CCGAGACTTG 
GACAAGCCCC GTCGCTCTCC CACACTGCGC TACAGGAGGA GTAGGAGCCG GTCTCCCAGA 
AGGCGGAGTC GATCTCCCAA AAGGAGAAGT CTGACCAGTC TTTTCTACAG CCCCTCCCCT 
CGCCGAGAAA GGCATCGGAG CAAGAGTCCA AGACGTCACC GCAGCAGGTC CCGAGATCGG 
CGGCACAGAT CCCGTTCCAA GTCCCCAGGT CATCACCGTA GTCACAGACA CAGGAGCCAC 
TCAAAGTCTC CCGAAAGGTC TAAGAAGAGC CACAAGAAGA GCCGGAGAGG GAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540617.1
CDS	204..1163
Translation	MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTAELVVDKAMELRFVGGVYGGNIKPTPFLCLTLKMLQIQPEKDIIVEFIKNEDFKYVRMLGALYMRLTGTAIDCYKYLEPLYNDYRKIKSQNRNGEFELMHVDEFIDELLHSERVCDIILPRLQKRYVLEEAEQLEPRVSALEEDMDDVESSEEEEEEDEKLERVPSPDHRRRSYRDLDKPRRSPTLRYRRSRSRSPRRRSRSPKRRSLTSLFYSPSPRRERHRSKSPRRHRSRSRDRRHRSRSKSPGHHRSHRHRSHSKSPERSKKSHKKSRRGNE

Target ORF information:

RefSeq Version	XM_011542315.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pre-mRNA processing factor 38A (PRPF38A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542315.2

ORF Insert Sequence:

ATGGCTAACC GTACAGTGAA GGATGCGCAC AGCATCCATG GCACCAACCC TCAATATCTG 
GTGGAGAAGA TCATTCGAAC GCGAATCTAT GAGTCCAAGT ACTGGAAAGA GGAGTGCTTT 
GGACTTACAG CTGAACTTGT AGTCGATAAA GCCATGGAGT TAAGGTTTGT GGGTGGCGTC 
TATGGTGGCA ACATAAAACC AACACCCTTT CTGTGTTTAA CCTTGAAGAT GCTTCAAATT 
CAACCCGAGA AGGATATCAT TGTAGAGTTT ATCAAAAATG AAGATTTCAA GTATGTCCGC 
ATGCTGGGGG CACTTTACAT GAGGCTGACA GGCACTGCAA TTGATTGCTA CAAGTACTTG 
GAACCTTTGT ACAATGACTA TCGAAAAATC AAGAGCCAGA ACCGAAATGG GGAGTTTGAA 
TTGATGCATG TTGATGAGTT TATTGATGAA CTATTGCACA GTGAGAGAGT CTGTGATATC 
ATTCTGCCCC GACTACAGAA ACGCTATGTA TTAGAGGAAG CTGAGCAACT GGAGCCTCGA 
GTTAGTGCTC TGGAAGAGGA CATGGATGAT GTGGAGTCCA GTGAAGAGGA AGAAGAGGAG 
GATGAGAAGT TGGAAAGAGT GCCATCACCT GATCACCGCC GGAGAAGCTA CCGAGACTTG 
GACAAGCCCC GTCGCTCTCC CACACTGCGC TACAGGAGGA GTAGGAGCCG GTCTCCCAGA 
AGGCGGAGTC GATCTCCCAA AAGGAGAAGT CTGACCAGTC TTTTCTACAG CCCCTCCCCT 
CGCCGAGAAA GGCATCGGAG CAAGAGTCCA AGACGTCACC GCAGCAGGTC CCGAGATCGG 
CGGCACAGAT CCCGTTCCAA GTCCCCAGGT CATCACCGTA GTCACAGACA CAGGAGCCAC 
TCAAAGTCTC CCGAAAGGTC TAAGAAGAGC CACAAGAAGA GCCGGAGAGG GAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF38A ( XM_011542315.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540617.1 Homo sapiens pre-mRNA processing factor 38A (PRPF38A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu73888
Clone ID Related Accession (Same CDS sequence)	XM_011542315.2 , XM_011542315.3
Accession Version	XM_011542315.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor 38A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011542315.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACC GTACAGTGAA GGATGCGCAC AGCATCCATG GCACCAACCC TCAATATCTG 
GTGGAGAAGA TCATTCGAAC GCGAATCTAT GAGTCCAAGT ACTGGAAAGA GGAGTGCTTT 
GGACTTACAG CTGAACTTGT AGTCGATAAA GCCATGGAGT TAAGGTTTGT GGGTGGCGTC 
TATGGTGGCA ACATAAAACC AACACCCTTT CTGTGTTTAA CCTTGAAGAT GCTTCAAATT 
CAACCCGAGA AGGATATCAT TGTAGAGTTT ATCAAAAATG AAGATTTCAA GTATGTCCGC 
ATGCTGGGGG CACTTTACAT GAGGCTGACA GGCACTGCAA TTGATTGCTA CAAGTACTTG 
GAACCTTTGT ACAATGACTA TCGAAAAATC AAGAGCCAGA ACCGAAATGG GGAGTTTGAA 
TTGATGCATG TTGATGAGTT TATTGATGAA CTATTGCACA GTGAGAGAGT CTGTGATATC 
ATTCTGCCCC GACTACAGAA ACGCTATGTA TTAGAGGAAG CTGAGCAACT GGAGCCTCGA 
GTTAGTGCTC TGGAAGAGGA CATGGATGAT GTGGAGTCCA GTGAAGAGGA AGAAGAGGAG 
GATGAGAAGT TGGAAAGAGT GCCATCACCT GATCACCGCC GGAGAAGCTA CCGAGACTTG 
GACAAGCCCC GTCGCTCTCC CACACTGCGC TACAGGAGGA GTAGGAGCCG GTCTCCCAGA 
AGGCGGAGTC GATCTCCCAA AAGGAGAAGT CTGACCAGTC TTTTCTACAG CCCCTCCCCT 
CGCCGAGAAA GGCATCGGAG CAAGAGTCCA AGACGTCACC GCAGCAGGTC CCGAGATCGG 
CGGCACAGAT CCCGTTCCAA GTCCCCAGGT CATCACCGTA GTCACAGACA CAGGAGCCAC 
TCAAAGTCTC CCGAAAGGTC TAAGAAGAGC CACAAGAAGA GCCGGAGAGG GAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540617.1
CDS	187..1146
Translation	MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTAELVVDKAMELRFVGGVYGGNIKPTPFLCLTLKMLQIQPEKDIIVEFIKNEDFKYVRMLGALYMRLTGTAIDCYKYLEPLYNDYRKIKSQNRNGEFELMHVDEFIDELLHSERVCDIILPRLQKRYVLEEAEQLEPRVSALEEDMDDVESSEEEEEEDEKLERVPSPDHRRRSYRDLDKPRRSPTLRYRRSRSRSPRRRSRSPKRRSLTSLFYSPSPRRERHRSKSPRRHRSRSRDRRHRSRSKSPGHHRSHRHRSHSKSPERSKKSHKKSRRGNE

Target ORF information:

RefSeq Version	XM_011542315.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pre-mRNA processing factor 38A (PRPF38A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542315.3

ORF Insert Sequence:

ATGGCTAACC GTACAGTGAA GGATGCGCAC AGCATCCATG GCACCAACCC TCAATATCTG 
GTGGAGAAGA TCATTCGAAC GCGAATCTAT GAGTCCAAGT ACTGGAAAGA GGAGTGCTTT 
GGACTTACAG CTGAACTTGT AGTCGATAAA GCCATGGAGT TAAGGTTTGT GGGTGGCGTC 
TATGGTGGCA ACATAAAACC AACACCCTTT CTGTGTTTAA CCTTGAAGAT GCTTCAAATT 
CAACCCGAGA AGGATATCAT TGTAGAGTTT ATCAAAAATG AAGATTTCAA GTATGTCCGC 
ATGCTGGGGG CACTTTACAT GAGGCTGACA GGCACTGCAA TTGATTGCTA CAAGTACTTG 
GAACCTTTGT ACAATGACTA TCGAAAAATC AAGAGCCAGA ACCGAAATGG GGAGTTTGAA 
TTGATGCATG TTGATGAGTT TATTGATGAA CTATTGCACA GTGAGAGAGT CTGTGATATC 
ATTCTGCCCC GACTACAGAA ACGCTATGTA TTAGAGGAAG CTGAGCAACT GGAGCCTCGA 
GTTAGTGCTC TGGAAGAGGA CATGGATGAT GTGGAGTCCA GTGAAGAGGA AGAAGAGGAG 
GATGAGAAGT TGGAAAGAGT GCCATCACCT GATCACCGCC GGAGAAGCTA CCGAGACTTG 
GACAAGCCCC GTCGCTCTCC CACACTGCGC TACAGGAGGA GTAGGAGCCG GTCTCCCAGA 
AGGCGGAGTC GATCTCCCAA AAGGAGAAGT CTGACCAGTC TTTTCTACAG CCCCTCCCCT 
CGCCGAGAAA GGCATCGGAG CAAGAGTCCA AGACGTCACC GCAGCAGGTC CCGAGATCGG 
CGGCACAGAT CCCGTTCCAA GTCCCCAGGT CATCACCGTA GTCACAGACA CAGGAGCCAC 
TCAAAGTCTC CCGAAAGGTC TAAGAAGAGC CACAAGAAGA GCCGGAGAGG GAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,L?hrmann R,Stark H

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

Multiple protein-protein interactions converging on the Prp38 protein during activation of the human spliceosome.
RNA (New York, N.Y.)22(2)265-77(2016 Feb)
Sch?tze T,Ulrich AK,Apelt L,Will CL,Bartlick N,Seeger M,Weber G,L?hrmann R,Stelzl U,Wahl MC

A Y2H-seq approach defines the human protein methyltransferase interactome.
Nature methods10(4)339-42(2013 Apr)
Weimann M,Grossmann A,Woodsmith J,?zkan Z,Birth P,Meierhofer D,Benlasfer N,Valovka T,Timmermann B,Wanker EE,Sauer S,Stelzl U

The protein interaction landscape of the human CMGC kinase group.
Cell reports3(4)1306-20(2013 Apr)
Varjosalo M,Keskitalo S,Van Drogen A,Nurkkala H,Vichalkovski A,Aebersold R,Gstaiger M

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PRPF38A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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