PHF5A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHF5A
Entrez Gene ID	84844
Full Name	PHD finger protein 5A
Synonyms	INI,Rds3,SAP14b,SF3B7,SF3b14b,bK223H9.2
General protein information	Preferred Names PHD finger protein 5A Names PHD finger-like domain-containing protein 5A PHD finger-like domain protein 5A PHD-finger 5a splicing factor 3B associated 14 kDa protein splicing factor 3b, subunit 7
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.2
Summary	This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_032758.3	NP_116147.1	PHD finger-like domain-containing protein 5A
NM_032758.4	NP_116147.1	PHD finger-like domain-containing protein 5A

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RDS3	NP_015419.1
Arabidopsis thaliana (thale cress)	AT1G07170	NP_001077473.1
Oryza sativa (rice)	Os05g0367000	NP_001055334.1
Xenopus tropicalis (tropical clawed frog)	phf5a	NP_001123712.1
Pan troglodytes (chimpanzee)	PHF5A	XP_003317310.1
Drosophila melanogaster (fruit fly)	CG9548	NP_609038.1
Bos taurus (cattle)	PHF5A	NP_001076851.1
Gallus gallus (chicken)	PHF5A	NP_001264305.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009905	XP_319026.1
Caenorhabditis elegans (roundworm)	phf-5	NP_001022909.1
Kluyveromyces lactis	KLLA0C15675g	XP_452900.1
Magnaporthe oryzae (rice blast fungus)	MGG_05173	XP_003712711.1
Arabidopsis thaliana (thale cress)	AT2G30000	NP_565691.1
Oryza sativa (rice)	Os04g0663300	NP_001054163.1
Mus musculus (house mouse)	Phf5a	NP_081013.1
Danio rerio (zebrafish)	phf5a	NP_775373.1
Schizosaccharomyces pombe (fission yeast)	ini1	NP_593792.1
Neurospora crassa	NCU00348	XP_957819.2
Homo sapiens (human)	PHF5A	NP_116147.1
	LOC711683	XP_002798444.1
Canis lupus familiaris (dog)	PHF5A	XP_531718.1
Rattus norvegicus (Norway rat)	Phf5a	NP_620243.1
Eremothecium gossypii	AGOS_ABR103C	NP_983050.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of the human activated spliceosome in three conformational states.
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell research(2018 Jan)

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.
Teng T, Tsai JH, Puyang X, Seiler M, Peng S, Prajapati S, Aird D, Buonamici S, Caleb B, Chan B, Corson L, Feala J, Fekkes P, Gerard B, Karr C, Korpal M, Liu X, T Lowe J, Mizui Y, Palacino J, Park E, Smith PG, Subramanian V, Wu ZJ, Zou J, Yu L, Chicas A, Warmuth M, Larsen N, Zhu P
Nature communications815522(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found PHF5A, a component of the U2 snRNP mRNA splicing factor, blocks expression from recombinant Adeno-associated virus vectors
Title: An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses.

PHF5A facilitates recognition of exons with unusual C-rich 3' splice sites in thousands of essential genes
Title: Genome-wide RNAi screens in human brain tumor isolates reveal a novel viability requirement for PHF5A.

The molecular and genetic research in this paper is performed on mouse but the paper identifies and discusses homologues found in human genomic sequence databases.
Title: Identification and characterization of a novel murine multigene family containing a PHD-finger-like motif.

The following PHF5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHF5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11415	NM_032758.4 Latest version!	Homo sapiens PHD finger protein 5A (PHF5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu11415	NM_032758.3	Homo sapiens PHD finger protein 5A (PHF5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHF5A ( NM_032758.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116147.1 Homo sapiens PHD finger protein 5A (PHF5A), mRNA.

Price & Availability↑

CloneID	OHu11415
Clone ID Related Accession (Same CDS sequence)	NM_032758.3 , NM_032758.4
Accession Version	NM_032758.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Homo sapiens(human)
Product	PHD finger-like domain-containing protein 5A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU584712.1, AL008582.11 and BC007321.2. On Nov 22, 2004 this sequence version replaced NM_032758.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007321.2, SRR1163658.224193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTAAAC ATCATCCTGA TTTGATCTTT TGCCGCAAGC AGGCTGGTGT TGCCATCGGA 
AGACTGTGTG AAAAATGTGA TGGCAAGTGT GTGATTTGTG ACTCCTATGT GCGTCCCTGC 
ACTCTGGTGC GCATATGTGA TGAGTGTAAC TATGGATCTT ACCAGGGGCG CTGTGTGATC 
TGTGGAGGAC CTGGGGTCTC TGATGCCTAT TATTGTAAGG AGTGCACCAT CCAGGAGAAG 
GACAGAGATG GCTGCCCAAA GATTGTCAAT CTGGGGAGCT CTAAGACAGA CCTCTTCTAT 
GAACGCAAAA AATACGGCTT CAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116147.1
CDS	52..384
Translation	MAKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYERKKYGFKKR

Target ORF information:

RefSeq Version	NM_032758.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD finger protein 5A (PHF5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032758.3

ORF Insert Sequence:

ATGGCTAAAC ATCATCCTGA TTTGATCTTT TGCCGCAAGC AGGCTGGTGT TGCCATCGGA 
AGACTGTGTG AAAAATGTGA TGGCAAGTGT GTGATTTGTG ACTCCTATGT GCGTCCCTGC 
ACTCTGGTGC GCATATGTGA TGAGTGTAAC TATGGATCTT ACCAGGGGCG CTGTGTGATC 
TGTGGAGGAC CTGGGGTCTC TGATGCCTAT TATTGTAAGG AGTGCACCAT CCAGGAGAAG 
GACAGAGATG GCTGCCCAAA GATTGTCAAT CTGGGGAGCT CTAAGACAGA CCTCTTCTAT 
GAACGCAAAA AATACGGCTT CAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHF5A ( NM_032758.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_116147.1 Homo sapiens PHD finger protein 5A (PHF5A), mRNA.

Price & Availability↑

CloneID	OHu11415
Clone ID Related Accession (Same CDS sequence)	NM_032758.3 , NM_032758.4
Accession Version	NM_032758.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	PHD finger-like domain-containing protein 5A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU584712.1, AL008582.11 and BC007321.2. On Nov 23, 2018 this sequence version replaced NM_032758.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189658.132999.1, SRR1163658.184099.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216252.4/ ENSP00000216252.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTAAAC ATCATCCTGA TTTGATCTTT TGCCGCAAGC AGGCTGGTGT TGCCATCGGA 
AGACTGTGTG AAAAATGTGA TGGCAAGTGT GTGATTTGTG ACTCCTATGT GCGTCCCTGC 
ACTCTGGTGC GCATATGTGA TGAGTGTAAC TATGGATCTT ACCAGGGGCG CTGTGTGATC 
TGTGGAGGAC CTGGGGTCTC TGATGCCTAT TATTGTAAGG AGTGCACCAT CCAGGAGAAG 
GACAGAGATG GCTGCCCAAA GATTGTCAAT CTGGGGAGCT CTAAGACAGA CCTCTTCTAT 
GAACGCAAAA AATACGGCTT CAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116147.1
CDS	40..372
Translation	MAKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYERKKYGFKKR

Target ORF information:

RefSeq Version	NM_032758.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD finger protein 5A (PHF5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032758.4

ORF Insert Sequence:

ATGGCTAAAC ATCATCCTGA TTTGATCTTT TGCCGCAAGC AGGCTGGTGT TGCCATCGGA 
AGACTGTGTG AAAAATGTGA TGGCAAGTGT GTGATTTGTG ACTCCTATGT GCGTCCCTGC 
ACTCTGGTGC GCATATGTGA TGAGTGTAAC TATGGATCTT ACCAGGGGCG CTGTGTGATC 
TGTGGAGGAC CTGGGGTCTC TGATGCCTAT TATTGTAAGG AGTGCACCAT CCAGGAGAAG 
GACAGAGATG GCTGCCCAAA GATTGTCAAT CTGGGGAGCT CTAAGACAGA CCTCTTCTAT 
GAACGCAAAA AATACGGCTT CAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of the human activated spliceosome in three conformational states.
Cell research(2018 Jan)
Zhang X,Yan C,Zhan X,Li L,Lei J,Shi Y

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.
Nature communications815522(2017 May)
Teng T,Tsai JH,Puyang X,Seiler M,Peng S,Prajapati S,Aird D,Buonamici S,Caleb B,Chan B,Corson L,Feala J,Fekkes P,Gerard B,Karr C,Korpal M,Liu X,T Lowe J,Mizui Y,Palacino J,Park E,Smith PG,Subramanian V,Wu ZJ,Zou J,Yu L,Chicas A,Warmuth M,Larsen N,Zhu P

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PHF5A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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