GPT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPT2
Entrez Gene ID	84706
Full Name	glutamic--pyruvic transaminase 2
Synonyms	ALT2,GPT 2,MRT49
General protein information	Preferred Names glutamic--pyruvic transaminase 2 Names alanine aminotransferase 2 glutamate pyruvate transaminase 2 glutamic pyruvate transaminase (alanine aminotransferase) 2 glutamic pyruvate transaminase 2 glutamic--alanine transaminase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q11.2
Summary	This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015].
Disorder MIM:	138210
Disorder Html:	Mental retardation, autosomal recessive 49, 616281 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017023790.1	XP_016879279.1	alanine aminotransferase 2 isoform X1
NM_001142466.3	NP_001135938.1	alanine aminotransferase 2 isoform 2
NM_001142466.2	NP_001135938.1	alanine aminotransferase 2 isoform 2
NM_133443.4	NP_597700.1	alanine aminotransferase 2 isoform 1
NM_133443.3	NP_597700.1	alanine aminotransferase 2 isoform 1

Pathways from BioSystems

Homologies

	LOC716305	XP_001113757.2
Drosophila melanogaster (fruit fly)	CG1640	NP_572879.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000901	XP_316880.5
Neurospora crassa	NCU03973	XP_957612.1
Arabidopsis thaliana (thale cress)	AlaAT1	NP_173173.3
Oryza sativa (rice)	Os10g0390500	NP_001064504.1
Bos taurus (cattle)	GPT2	NP_001180116.1
Magnaporthe oryzae (rice blast fungus)	MGG_06503	XP_003717056.1
Mus musculus (house mouse)	Gpt2	NP_776291.1
Caenorhabditis elegans (roundworm)	CELE_C32F10.8	NP_001021021.1
Saccharomyces cerevisiae (baker's yeast)	ALT1	NP_013190.1
Schizosaccharomyces pombe (fission yeast)	SPBC582.08	NP_595176.1
Xenopus tropicalis (tropical clawed frog)	LOC100494152	XP_002931716.1
Homo sapiens (human)	GPT2	NP_597700.1
Rattus norvegicus (Norway rat)	Gpt2	NP_001012057.1
Arabidopsis thaliana (thale cress)	ALAAT2	NP_001185380.1
Pan troglodytes (chimpanzee)	GPT2	XP_003952890.1
Gallus gallus (chicken)	GPT2	XP_414111.3
Danio rerio (zebrafish)	gpt2	NP_001092227.2
Kluyveromyces lactis	KLLA0F19162g	XP_455940.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel compound heterozygous mutations in GPT2 linked to microcephaly, and intellectual developmental disability with or without spastic paraplegia.
Kaymakcalan H, Yarman Y, Goc N, Toy F, Meral C, Ercan-Sencicek AG, Gunel M
American journal of medical genetics. Part A176(2)421-425(2018 Feb)

Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.
Cao Y, Lin SH, Wang Y, Chin YE, Kang L, Mi J
Theranostics7(12)3021-3033(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Recessively inherited loss of function GPT2 mutations are a novel cause of intellectual disability.
Title: Loss of function mutation in glutamic pyruvate transaminase 2 (GPT2) causes developmental encephalopathy.

ATF4 silencing prevented the activating effect of histidinol and tunicamycin on ATF4 and ALT2 expression. Our findings point to ALT2 as an enzyme involved in the metabolic adaptation of the cell to stress
Title: Activating transcription factor 4 mediates up-regulation of alanine aminotransferase 2 gene expression under metabolic stress.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

A clinical method for selective measurement of ALT1 and 2 in human plasma is described.
Title: Detection of the mitochondrial and catalytically active alanine aminotransferase in human tissues and plasma.

Elevation of liver enzymes and hepatic insulin resistance as reflected by fasting insulin occur in the early stages of insulin resistance and highlight the central role of the liver in insulin resistance in the general population.
Title: A comparison of associations of alanine aminotransferase and gamma-glutamyltransferase with fasting glucose, fasting insulin, and glycated hemoglobin in women with and without diabetes.

The following GPT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31853	NM_133443.4 Latest version!	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu32089	NM_001142466.3 Latest version!	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu86326	XM_017023790.1 Latest version!	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu32089	NM_001142466.2	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu31853	NM_133443.3	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPT2 ( NM_133443.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_597700.1 Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31853
Clone ID Related Accession (Same CDS sequence)	NM_133443.4 , NM_133443.3
Accession Version	NM_133443.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	alanine aminotransferase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007225.2, HY117879.1, AY029173.2, AC018845.7 and BE466380.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_133443.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.105996.1, SRR1803611.20444.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 19360321; reported by MitoCarta MANE Ensembl match :: ENST00000340124.9/ ENSP00000345282.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGCGGG CGGCGGCGCT GGTCCGGCGG GGCTGTGGTC CCCGGACCCC CAGCTCCTGG 
GGCCGCAGCC AGAGCAGCGC GGCCGCCGAG GCCTCGGCGG TGCTCAAGGT GCGGCCCGAG 
CGCAGCCGGC GCGAGCGCAT CCTCACGCTG GAGTCCATGA ACCCGCAGGT GAAGGCGGTG 
GAGTACGCCG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGCT CGAGCTGCAG 
CGGGGTATCA AAAAGCCATT CACAGAGGTC ATCCGAGCCA ACATCGGGGA CGCCCAGGCT 
ATGGGGCAGC AGCCAATCAC CTTCCTCCGG CAGGTGATGG CACTATGCAC CTACCCAAAC 
CTGCTGGACA GCCCCAGCTT CCCAGAAGAT GCTAAGAAAC GTGCCCGGCG GATCCTGCAG 
GCTTGTGGCG GGAACAGCCT GGGGTCCTAC AGTGCTAGCC AGGGTGTCAA CTGCATCCGT 
GAAGATGTGG CTGCCTACAT CACCAGGAGG GATGGCGGTG TGCCTGCGGA CCCCGACAAC 
ATCTACCTGA CCACGGGAGC TAGTGACGGC ATTTCTACGA TCCTGAAGAT CCTCGTCTCC 
GGGGGCGGCA AGTCACGGAC AGGTGTGATG ATCCCCATCC CACAATATCC CCTCTATTCA 
GCTGTCATCT CTGAGCTCGA CGCCATCCAG GTGAATTACT ACCTGGACGA GGAGAACTGC 
TGGGCGCTGA ATGTGAATGA GCTCCGGCGG GCGGTGCAGG AGGCCAAAGA CCACTGTGAT 
CCTAAGGTGC TCTGCATAAT CAACCCTGGG AACCCCACAG GCCAGGTACA AAGCAGAAAG 
TGCATAGAAG ATGTGATCCA CTTTGCCTGG GAAGAGAAGC TCTTTCTCCT GGCTGATGAG 
GTGTACCAGG ACAACGTGTA CTCTCCAGAT TGCAGATTCC ACTCCTTCAA GAAGGTGCTG 
TACGAGATGG GGCCCGAGTA CTCCAGCAAC GTGGAGCTCG CCTCCTTCCA CTCCACCTCC 
AAGGGCTACA TGGGCGAGTG TGGTTACAGA GGAGGCTACA TGGAGGTGAT CAACCTGCAC 
CCTGAGATCA AGGGCCAGCT GGTGAAGCTG CTGTCGGTGC GCCTGTGCCC CCCAGTGTCT 
GGGCAGGCCG CCATGGACAT TGTCGTGAAC CCCCCGGTGG CAGGAGAGGA GTCCTTTGAG 
CAATTCAGCC GAGAGAAGGA GTCGGTCCTG GGTAATCTGG CCAAAAAAGC AAAGCTGACG 
GAAGACCTGT TTAACCAAGT CCCAGGAATT CACTGCAACC CCTTGCAGGG GGCCATGTAC 
GCCTTCCCTC GGATCTTCAT TCCTGCCAAA GCTGTGGAGG CTGCTCAGGC CCATCAAATG 
GCTCCAGACA TGTTCTACTG CATGAAGCTC CTGGAGGAGA CTGGCATCTG TGTCGTGCCC 
GGCAGTGGCT TTGGGCAGAG GGAAGGCACT TACCACTTCA GGATGACTAT CCTCCCTCCA 
GTGGAGAAGC TGAAAACGGT GCTGCAGAAG GTGAAAGACT TCCACATCAA CTTCCTGGAG 
AAGTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_597700.1
CDS	129..1700
Translation	MQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVLKAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA

Target ORF information:

RefSeq Version	NM_133443.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133443.4

ORF Insert Sequence:

ATGCAGCGGG CGGCGGCGCT GGTCCGGCGG GGCTGTGGTC CCCGGACCCC CAGCTCCTGG 
GGCCGCAGCC AGAGCAGCGC GGCCGCCGAG GCCTCGGCGG TGCTCAAGGT GCGGCCCGAG 
CGCAGCCGGC GCGAGCGCAT CCTCACGCTG GAGTCCATGA ACCCGCAGGT GAAGGCGGTG 
GAGTACGCCG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGCT CGAGCTGCAG 
CGGGGTATCA AAAAGCCATT CACAGAGGTC ATCCGAGCCA ACATCGGGGA CGCCCAGGCT 
ATGGGGCAGC AGCCAATCAC CTTCCTCCGG CAGGTGATGG CACTATGCAC CTACCCAAAC 
CTGCTGGACA GCCCCAGCTT CCCAGAAGAT GCTAAGAAAC GTGCCCGGCG GATCCTGCAG 
GCTTGTGGCG GGAACAGCCT GGGGTCCTAC AGTGCTAGCC AGGGTGTCAA CTGCATCCGT 
GAAGATGTGG CTGCCTACAT CACCAGGAGG GATGGCGGTG TGCCTGCGGA CCCCGACAAC 
ATCTACCTGA CCACGGGAGC TAGTGACGGC ATTTCTACGA TCCTGAAGAT CCTCGTCTCC 
GGGGGCGGCA AGTCACGGAC AGGTGTGATG ATCCCCATCC CACAATATCC CCTCTATTCA 
GCTGTCATCT CTGAGCTCGA CGCCATCCAG GTGAATTACT ACCTGGACGA GGAGAACTGC 
TGGGCGCTGA ATGTGAATGA GCTCCGGCGG GCGGTGCAGG AGGCCAAAGA CCACTGTGAT 
CCTAAGGTGC TCTGCATAAT CAACCCTGGG AACCCCACAG GCCAGGTACA AAGCAGAAAG 
TGCATAGAAG ATGTGATCCA CTTTGCCTGG GAAGAGAAGC TCTTTCTCCT GGCTGATGAG 
GTGTACCAGG ACAACGTGTA CTCTCCAGAT TGCAGATTCC ACTCCTTCAA GAAGGTGCTG 
TACGAGATGG GGCCCGAGTA CTCCAGCAAC GTGGAGCTCG CCTCCTTCCA CTCCACCTCC 
AAGGGCTACA TGGGCGAGTG TGGTTACAGA GGAGGCTACA TGGAGGTGAT CAACCTGCAC 
CCTGAGATCA AGGGCCAGCT GGTGAAGCTG CTGTCGGTGC GCCTGTGCCC CCCAGTGTCT 
GGGCAGGCCG CCATGGACAT TGTCGTGAAC CCCCCGGTGG CAGGAGAGGA GTCCTTTGAG 
CAATTCAGCC GAGAGAAGGA GTCGGTCCTG GGTAATCTGG CCAAAAAAGC AAAGCTGACG 
GAAGACCTGT TTAACCAAGT CCCAGGAATT CACTGCAACC CCTTGCAGGG GGCCATGTAC 
GCCTTCCCTC GGATCTTCAT TCCTGCCAAA GCTGTGGAGG CTGCTCAGGC CCATCAAATG 
GCTCCAGACA TGTTCTACTG CATGAAGCTC CTGGAGGAGA CTGGCATCTG TGTCGTGCCC 
GGCAGTGGCT TTGGGCAGAG GGAAGGCACT TACCACTTCA GGATGACTAT CCTCCCTCCA 
GTGGAGAAGC TGAAAACGGT GCTGCAGAAG GTGAAAGACT TCCACATCAA CTTCCTGGAG 
AAGTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT2 ( NM_133443.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_597700.1 Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31853
Clone ID Related Accession (Same CDS sequence)	NM_133443.4 , NM_133443.3
Accession Version	NM_133443.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	alanine aminotransferase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY117879.1, AY029173.2, AC018845.7 and BE466380.1. This sequence is a reference standard in the RefSeqGene project. On Apr 9, 2015 this sequence version replaced NM_133443.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803615.113149.1, SRR1660809.105996.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 19360321; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGCGGG CGGCGGCGCT GGTCCGGCGG GGCTGTGGTC CCCGGACCCC CAGCTCCTGG 
GGCCGCAGCC AGAGCAGCGC GGCCGCCGAG GCCTCGGCGG TGCTCAAGGT GCGGCCCGAG 
CGCAGCCGGC GCGAGCGCAT CCTCACGCTG GAGTCCATGA ACCCGCAGGT GAAGGCGGTG 
GAGTACGCCG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGCT CGAGCTGCAG 
CGGGGTATCA AAAAGCCATT CACAGAGGTC ATCCGAGCCA ACATCGGGGA CGCCCAGGCT 
ATGGGGCAGC AGCCAATCAC CTTCCTCCGG CAGGTGATGG CACTATGCAC CTACCCAAAC 
CTGCTGGACA GCCCCAGCTT CCCAGAAGAT GCTAAGAAAC GTGCCCGGCG GATCCTGCAG 
GCTTGTGGCG GGAACAGCCT GGGGTCCTAC AGTGCTAGCC AGGGTGTCAA CTGCATCCGT 
GAAGATGTGG CTGCCTACAT CACCAGGAGG GATGGCGGTG TGCCTGCGGA CCCCGACAAC 
ATCTACCTGA CCACGGGAGC TAGTGACGGC ATTTCTACGA TCCTGAAGAT CCTCGTCTCC 
GGGGGCGGCA AGTCACGGAC AGGTGTGATG ATCCCCATCC CACAATATCC CCTCTATTCA 
GCTGTCATCT CTGAGCTCGA CGCCATCCAG GTGAATTACT ACCTGGACGA GGAGAACTGC 
TGGGCGCTGA ATGTGAATGA GCTCCGGCGG GCGGTGCAGG AGGCCAAAGA CCACTGTGAT 
CCTAAGGTGC TCTGCATAAT CAACCCTGGG AACCCCACAG GCCAGGTACA AAGCAGAAAG 
TGCATAGAAG ATGTGATCCA CTTTGCCTGG GAAGAGAAGC TCTTTCTCCT GGCTGATGAG 
GTGTACCAGG ACAACGTGTA CTCTCCAGAT TGCAGATTCC ACTCCTTCAA GAAGGTGCTG 
TACGAGATGG GGCCCGAGTA CTCCAGCAAC GTGGAGCTCG CCTCCTTCCA CTCCACCTCC 
AAGGGCTACA TGGGCGAGTG TGGTTACAGA GGAGGCTACA TGGAGGTGAT CAACCTGCAC 
CCTGAGATCA AGGGCCAGCT GGTGAAGCTG CTGTCGGTGC GCCTGTGCCC CCCAGTGTCT 
GGGCAGGCCG CCATGGACAT TGTCGTGAAC CCCCCGGTGG CAGGAGAGGA GTCCTTTGAG 
CAATTCAGCC GAGAGAAGGA GTCGGTCCTG GGTAATCTGG CCAAAAAAGC AAAGCTGACG 
GAAGACCTGT TTAACCAAGT CCCAGGAATT CACTGCAACC CCTTGCAGGG GGCCATGTAC 
GCCTTCCCTC GGATCTTCAT TCCTGCCAAA GCTGTGGAGG CTGCTCAGGC CCATCAAATG 
GCTCCAGACA TGTTCTACTG CATGAAGCTC CTGGAGGAGA CTGGCATCTG TGTCGTGCCC 
GGCAGTGGCT TTGGGCAGAG GGAAGGCACT TACCACTTCA GGATGACTAT CCTCCCTCCA 
GTGGAGAAGC TGAAAACGGT GCTGCAGAAG GTGAAAGACT TCCACATCAA CTTCCTGGAG 
AAGTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_597700.1
CDS	111..1682
Translation	MQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVLKAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA

Target ORF information:

RefSeq Version	NM_133443.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133443.3

ORF Insert Sequence:

ATGCAGCGGG CGGCGGCGCT GGTCCGGCGG GGCTGTGGTC CCCGGACCCC CAGCTCCTGG 
GGCCGCAGCC AGAGCAGCGC GGCCGCCGAG GCCTCGGCGG TGCTCAAGGT GCGGCCCGAG 
CGCAGCCGGC GCGAGCGCAT CCTCACGCTG GAGTCCATGA ACCCGCAGGT GAAGGCGGTG 
GAGTACGCCG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGCT CGAGCTGCAG 
CGGGGTATCA AAAAGCCATT CACAGAGGTC ATCCGAGCCA ACATCGGGGA CGCCCAGGCT 
ATGGGGCAGC AGCCAATCAC CTTCCTCCGG CAGGTGATGG CACTATGCAC CTACCCAAAC 
CTGCTGGACA GCCCCAGCTT CCCAGAAGAT GCTAAGAAAC GTGCCCGGCG GATCCTGCAG 
GCTTGTGGCG GGAACAGCCT GGGGTCCTAC AGTGCTAGCC AGGGTGTCAA CTGCATCCGT 
GAAGATGTGG CTGCCTACAT CACCAGGAGG GATGGCGGTG TGCCTGCGGA CCCCGACAAC 
ATCTACCTGA CCACGGGAGC TAGTGACGGC ATTTCTACGA TCCTGAAGAT CCTCGTCTCC 
GGGGGCGGCA AGTCACGGAC AGGTGTGATG ATCCCCATCC CACAATATCC CCTCTATTCA 
GCTGTCATCT CTGAGCTCGA CGCCATCCAG GTGAATTACT ACCTGGACGA GGAGAACTGC 
TGGGCGCTGA ATGTGAATGA GCTCCGGCGG GCGGTGCAGG AGGCCAAAGA CCACTGTGAT 
CCTAAGGTGC TCTGCATAAT CAACCCTGGG AACCCCACAG GCCAGGTACA AAGCAGAAAG 
TGCATAGAAG ATGTGATCCA CTTTGCCTGG GAAGAGAAGC TCTTTCTCCT GGCTGATGAG 
GTGTACCAGG ACAACGTGTA CTCTCCAGAT TGCAGATTCC ACTCCTTCAA GAAGGTGCTG 
TACGAGATGG GGCCCGAGTA CTCCAGCAAC GTGGAGCTCG CCTCCTTCCA CTCCACCTCC 
AAGGGCTACA TGGGCGAGTG TGGTTACAGA GGAGGCTACA TGGAGGTGAT CAACCTGCAC 
CCTGAGATCA AGGGCCAGCT GGTGAAGCTG CTGTCGGTGC GCCTGTGCCC CCCAGTGTCT 
GGGCAGGCCG CCATGGACAT TGTCGTGAAC CCCCCGGTGG CAGGAGAGGA GTCCTTTGAG 
CAATTCAGCC GAGAGAAGGA GTCGGTCCTG GGTAATCTGG CCAAAAAAGC AAAGCTGACG 
GAAGACCTGT TTAACCAAGT CCCAGGAATT CACTGCAACC CCTTGCAGGG GGCCATGTAC 
GCCTTCCCTC GGATCTTCAT TCCTGCCAAA GCTGTGGAGG CTGCTCAGGC CCATCAAATG 
GCTCCAGACA TGTTCTACTG CATGAAGCTC CTGGAGGAGA CTGGCATCTG TGTCGTGCCC 
GGCAGTGGCT TTGGGCAGAG GGAAGGCACT TACCACTTCA GGATGACTAT CCTCCCTCCA 
GTGGAGAAGC TGAAAACGGT GCTGCAGAAG GTGAAAGACT TCCACATCAA CTTCCTGGAG 
AAGTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT2 ( NM_001142466.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135938.1 Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu32089
Clone ID Related Accession (Same CDS sequence)	NM_001142466.3 , NM_001142466.2
Accession Version	NM_001142466.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	alanine aminotransferase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007225.2, DA400677.1, AY029173.2, AC018845.7 and BE466380.1. On May 31, 2019 this sequence version replaced NM_001142466.2. Transcript Variant: This variant (2) uses alternate exons in the 5' UTR and 5' coding sequence, resulting in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.80918.1, SRR1660805.81407.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 19360321; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC AGCCAATCAC CTTCCTCCGG CAGGTGATGG CACTATGCAC CTACCCAAAC 
CTGCTGGACA GCCCCAGCTT CCCAGAAGAT GCTAAGAAAC GTGCCCGGCG GATCCTGCAG 
GCTTGTGGCG GGAACAGCCT GGGGTCCTAC AGTGCTAGCC AGGGTGTCAA CTGCATCCGT 
GAAGATGTGG CTGCCTACAT CACCAGGAGG GATGGCGGTG TGCCTGCGGA CCCCGACAAC 
ATCTACCTGA CCACGGGAGC TAGTGACGGC ATTTCTACGA TCCTGAAGAT CCTCGTCTCC 
GGGGGCGGCA AGTCACGGAC AGGTGTGATG ATCCCCATCC CACAATATCC CCTCTATTCA 
GCTGTCATCT CTGAGCTCGA CGCCATCCAG GTGAATTACT ACCTGGACGA GGAGAACTGC 
TGGGCGCTGA ATGTGAATGA GCTCCGGCGG GCGGTGCAGG AGGCCAAAGA CCACTGTGAT 
CCTAAGGTGC TCTGCATAAT CAACCCTGGG AACCCCACAG GCCAGGTACA AAGCAGAAAG 
TGCATAGAAG ATGTGATCCA CTTTGCCTGG GAAGAGAAGC TCTTTCTCCT GGCTGATGAG 
GTGTACCAGG ACAACGTGTA CTCTCCAGAT TGCAGATTCC ACTCCTTCAA GAAGGTGCTG 
TACGAGATGG GGCCCGAGTA CTCCAGCAAC GTGGAGCTCG CCTCCTTCCA CTCCACCTCC 
AAGGGCTACA TGGGCGAGTG TGGTTACAGA GGAGGCTACA TGGAGGTGAT CAACCTGCAC 
CCTGAGATCA AGGGCCAGCT GGTGAAGCTG CTGTCGGTGC GCCTGTGCCC CCCAGTGTCT 
GGGCAGGCCG CCATGGACAT TGTCGTGAAC CCCCCGGTGG CAGGAGAGGA GTCCTTTGAG 
CAATTCAGCC GAGAGAAGGA GTCGGTCCTG GGTAATCTGG CCAAAAAAGC AAAGCTGACG 
GAAGACCTGT TTAACCAAGT CCCAGGAATT CACTGCAACC CCTTGCAGGG GGCCATGTAC 
GCCTTCCCTC GGATCTTCAT TCCTGCCAAA GCTGTGGAGG CTGCTCAGGC CCATCAAATG 
GCTCCAGACA TGTTCTACTG CATGAAGCTC CTGGAGGAGA CTGGCATCTG TGTCGTGCCC 
GGCAGTGGCT TTGGGCAGAG GGAAGGCACT TACCACTTCA GGATGACTAT CCTCCCTCCA 
GTGGAGAAGC TGAAAACGGT GCTGCAGAAG GTGAAAGACT TCCACATCAA CTTCCTGGAG 
AAGTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135938.1
CDS	822..2093
Translation	MGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA

Target ORF information:

RefSeq Version	NM_001142466.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142466.3

ORF Insert Sequence:

ATGGGGCAGC AGCCAATCAC CTTCCTCCGG CAGGTGATGG CACTATGCAC CTACCCAAAC 
CTGCTGGACA GCCCCAGCTT CCCAGAAGAT GCTAAGAAAC GTGCCCGGCG GATCCTGCAG 
GCTTGTGGCG GGAACAGCCT GGGGTCCTAC AGTGCTAGCC AGGGTGTCAA CTGCATCCGT 
GAAGATGTGG CTGCCTACAT CACCAGGAGG GATGGCGGTG TGCCTGCGGA CCCCGACAAC 
ATCTACCTGA CCACGGGAGC TAGTGACGGC ATTTCTACGA TCCTGAAGAT CCTCGTCTCC 
GGGGGCGGCA AGTCACGGAC AGGTGTGATG ATCCCCATCC CACAATATCC CCTCTATTCA 
GCTGTCATCT CTGAGCTCGA CGCCATCCAG GTGAATTACT ACCTGGACGA GGAGAACTGC 
TGGGCGCTGA ATGTGAATGA GCTCCGGCGG GCGGTGCAGG AGGCCAAAGA CCACTGTGAT 
CCTAAGGTGC TCTGCATAAT CAACCCTGGG AACCCCACAG GCCAGGTACA AAGCAGAAAG 
TGCATAGAAG ATGTGATCCA CTTTGCCTGG GAAGAGAAGC TCTTTCTCCT GGCTGATGAG 
GTGTACCAGG ACAACGTGTA CTCTCCAGAT TGCAGATTCC ACTCCTTCAA GAAGGTGCTG 
TACGAGATGG GGCCCGAGTA CTCCAGCAAC GTGGAGCTCG CCTCCTTCCA CTCCACCTCC 
AAGGGCTACA TGGGCGAGTG TGGTTACAGA GGAGGCTACA TGGAGGTGAT CAACCTGCAC 
CCTGAGATCA AGGGCCAGCT GGTGAAGCTG CTGTCGGTGC GCCTGTGCCC CCCAGTGTCT 
GGGCAGGCCG CCATGGACAT TGTCGTGAAC CCCCCGGTGG CAGGAGAGGA GTCCTTTGAG 
CAATTCAGCC GAGAGAAGGA GTCGGTCCTG GGTAATCTGG CCAAAAAAGC AAAGCTGACG 
GAAGACCTGT TTAACCAAGT CCCAGGAATT CACTGCAACC CCTTGCAGGG GGCCATGTAC 
GCCTTCCCTC GGATCTTCAT TCCTGCCAAA GCTGTGGAGG CTGCTCAGGC CCATCAAATG 
GCTCCAGACA TGTTCTACTG CATGAAGCTC CTGGAGGAGA CTGGCATCTG TGTCGTGCCC 
GGCAGTGGCT TTGGGCAGAG GGAAGGCACT TACCACTTCA GGATGACTAT CCTCCCTCCA 
GTGGAGAAGC TGAAAACGGT GCTGCAGAAG GTGAAAGACT TCCACATCAA CTTCCTGGAG 
AAGTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT2 ( NM_001142466.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135938.1 Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 2, mRNA.

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CloneID	OHu32089
Clone ID Related Accession (Same CDS sequence)	NM_001142466.3 , NM_001142466.2
Accession Version	NM_001142466.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-12
Organism	Homo sapiens(human)
Product	alanine aminotransferase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA400677.1, AY029173.2, AC018845.7 and BE466380.1. On Apr 9, 2015 this sequence version replaced NM_001142466.1. Transcript Variant: This variant (2) uses alternate exons in the 5' UTR and 5' coding sequence, resulting in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK094971.1, SRR1803614.80918.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 19360321; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC AGCCAATCAC CTTCCTCCGG CAGGTGATGG CACTATGCAC CTACCCAAAC 
CTGCTGGACA GCCCCAGCTT CCCAGAAGAT GCTAAGAAAC GTGCCCGGCG GATCCTGCAG 
GCTTGTGGCG GGAACAGCCT GGGGTCCTAC AGTGCTAGCC AGGGTGTCAA CTGCATCCGT 
GAAGATGTGG CTGCCTACAT CACCAGGAGG GATGGCGGTG TGCCTGCGGA CCCCGACAAC 
ATCTACCTGA CCACGGGAGC TAGTGACGGC ATTTCTACGA TCCTGAAGAT CCTCGTCTCC 
GGGGGCGGCA AGTCACGGAC AGGTGTGATG ATCCCCATCC CACAATATCC CCTCTATTCA 
GCTGTCATCT CTGAGCTCGA CGCCATCCAG GTGAATTACT ACCTGGACGA GGAGAACTGC 
TGGGCGCTGA ATGTGAATGA GCTCCGGCGG GCGGTGCAGG AGGCCAAAGA CCACTGTGAT 
CCTAAGGTGC TCTGCATAAT CAACCCTGGG AACCCCACAG GCCAGGTACA AAGCAGAAAG 
TGCATAGAAG ATGTGATCCA CTTTGCCTGG GAAGAGAAGC TCTTTCTCCT GGCTGATGAG 
GTGTACCAGG ACAACGTGTA CTCTCCAGAT TGCAGATTCC ACTCCTTCAA GAAGGTGCTG 
TACGAGATGG GGCCCGAGTA CTCCAGCAAC GTGGAGCTCG CCTCCTTCCA CTCCACCTCC 
AAGGGCTACA TGGGCGAGTG TGGTTACAGA GGAGGCTACA TGGAGGTGAT CAACCTGCAC 
CCTGAGATCA AGGGCCAGCT GGTGAAGCTG CTGTCGGTGC GCCTGTGCCC CCCAGTGTCT 
GGGCAGGCCG CCATGGACAT TGTCGTGAAC CCCCCGGTGG CAGGAGAGGA GTCCTTTGAG 
CAATTCAGCC GAGAGAAGGA GTCGGTCCTG GGTAATCTGG CCAAAAAAGC AAAGCTGACG 
GAAGACCTGT TTAACCAAGT CCCAGGAATT CACTGCAACC CCTTGCAGGG GGCCATGTAC 
GCCTTCCCTC GGATCTTCAT TCCTGCCAAA GCTGTGGAGG CTGCTCAGGC CCATCAAATG 
GCTCCAGACA TGTTCTACTG CATGAAGCTC CTGGAGGAGA CTGGCATCTG TGTCGTGCCC 
GGCAGTGGCT TTGGGCAGAG GGAAGGCACT TACCACTTCA GGATGACTAT CCTCCCTCCA 
GTGGAGAAGC TGAAAACGGT GCTGCAGAAG GTGAAAGACT TCCACATCAA CTTCCTGGAG 
AAGTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135938.1
CDS	387..1658
Translation	MGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA

Target ORF information:

RefSeq Version	NM_001142466.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142466.2

ORF Insert Sequence:

ATGGGGCAGC AGCCAATCAC CTTCCTCCGG CAGGTGATGG CACTATGCAC CTACCCAAAC 
CTGCTGGACA GCCCCAGCTT CCCAGAAGAT GCTAAGAAAC GTGCCCGGCG GATCCTGCAG 
GCTTGTGGCG GGAACAGCCT GGGGTCCTAC AGTGCTAGCC AGGGTGTCAA CTGCATCCGT 
GAAGATGTGG CTGCCTACAT CACCAGGAGG GATGGCGGTG TGCCTGCGGA CCCCGACAAC 
ATCTACCTGA CCACGGGAGC TAGTGACGGC ATTTCTACGA TCCTGAAGAT CCTCGTCTCC 
GGGGGCGGCA AGTCACGGAC AGGTGTGATG ATCCCCATCC CACAATATCC CCTCTATTCA 
GCTGTCATCT CTGAGCTCGA CGCCATCCAG GTGAATTACT ACCTGGACGA GGAGAACTGC 
TGGGCGCTGA ATGTGAATGA GCTCCGGCGG GCGGTGCAGG AGGCCAAAGA CCACTGTGAT 
CCTAAGGTGC TCTGCATAAT CAACCCTGGG AACCCCACAG GCCAGGTACA AAGCAGAAAG 
TGCATAGAAG ATGTGATCCA CTTTGCCTGG GAAGAGAAGC TCTTTCTCCT GGCTGATGAG 
GTGTACCAGG ACAACGTGTA CTCTCCAGAT TGCAGATTCC ACTCCTTCAA GAAGGTGCTG 
TACGAGATGG GGCCCGAGTA CTCCAGCAAC GTGGAGCTCG CCTCCTTCCA CTCCACCTCC 
AAGGGCTACA TGGGCGAGTG TGGTTACAGA GGAGGCTACA TGGAGGTGAT CAACCTGCAC 
CCTGAGATCA AGGGCCAGCT GGTGAAGCTG CTGTCGGTGC GCCTGTGCCC CCCAGTGTCT 
GGGCAGGCCG CCATGGACAT TGTCGTGAAC CCCCCGGTGG CAGGAGAGGA GTCCTTTGAG 
CAATTCAGCC GAGAGAAGGA GTCGGTCCTG GGTAATCTGG CCAAAAAAGC AAAGCTGACG 
GAAGACCTGT TTAACCAAGT CCCAGGAATT CACTGCAACC CCTTGCAGGG GGCCATGTAC 
GCCTTCCCTC GGATCTTCAT TCCTGCCAAA GCTGTGGAGG CTGCTCAGGC CCATCAAATG 
GCTCCAGACA TGTTCTACTG CATGAAGCTC CTGGAGGAGA CTGGCATCTG TGTCGTGCCC 
GGCAGTGGCT TTGGGCAGAG GGAAGGCACT TACCACTTCA GGATGACTAT CCTCCCTCCA 
GTGGAGAAGC TGAAAACGGT GCTGCAGAAG GTGAAAGACT TCCACATCAA CTTCCTGGAG 
AAGTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT2 ( XM_017023790.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879279.1 Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant X1, mRNA.

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CloneID	OHu86326
Clone ID Related Accession (Same CDS sequence)	XM_017023790.1
Accession Version	XM_017023790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alanine aminotransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAATGG GGTCCTACAG TGCTAGCCAG GGTGTCAACT GCATCCGTGA AGATGTGGCT 
GCCTACATCA CCAGGAGGGA TGGCGGTGTG CCTGCGGACC CCGACAACAT CTACCTGACC 
ACGGGAGCTA GTGACGGCAT TTCTACGATC CTGAAGATCC TCGTCTCCGG GGGCGGCAAG 
TCACGGACAG GTGTGATGAT CCCCATCCCA CAATATCCCC TCTATTCAGC TGTCATCTCT 
GAGCTCGACG CCATCCAGGT GAATTACTAC CTGGACGAGG AGAACTGCTG GGCGCTGAAT 
GTGAATGAGC TCCGGCGGGC GGTGCAGGAG GCCAAAGACC ACTGTGATCC TAAGGTGCTC 
TGCATAATCA ACCCTGGGAA CCCCACAGGC CAGGTACAAA GCAGAAAGTG CATAGAAGAT 
GTGATCCACT TTGCCTGGGA AGAGAAGCTC TTTCTCCTGG CTGATGAGGT GTACCAGGAC 
AACGTGTACT CTCCAGATTG CAGATTCCAC TCCTTCAAGA AGGTGCTGTA CGAGATGGGG 
CCCGAGTACT CCAGCAACGT GGAGCTCGCC TCCTTCCACT CCACCTCCAA GGGCTACATG 
GGCGAGTGTG GTTACAGAGG AGGCTACATG GAGGTGATCA ACCTGCACCC TGAGATCAAG 
GGCCAGCTGG TGAAGCTGCT GTCGGTGCGC CTGTGCCCCC CAGTGTCTGG GCAGGCCGCC 
ATGGACATTG TCGTGAACCC CCCGGTGGCA GGAGAGGAGT CCTTTGAGCA ATTCAGCCGA 
GAGAAGGAGT CGGTCCTGGG TAATCTGGCC AAAAAAGCAA AGCTGACGGA AGACCTGTTT 
AACCAAGTCC CAGGAATTCA CTGCAACCCC TTGCAGGGGG CCATGTACGC CTTCCCTCGG 
ATCTTCATTC CTGCCAAAGC TGTGGAGGCT GCTCAGGCCC ATCAAATGGC TCCAGACATG 
TTCTACTGCA TGAAGCTCCT GGAGGAGACT GGCATCTGTG TCGTGCCCGG CAGTGGCTTT 
GGGCAGAGGG AAGGCACTTA CCACTTCAGG ATGACTATCC TCCCTCCAGT GGAGAAGCTG 
AAAACGGTGC TGCAGAAGGT GAAAGACTTC CACATCAACT TCCTGGAGAA GTACGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879279.1
CDS	16..1155
Translation	MRMGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA

Target ORF information:

RefSeq Version	XM_017023790.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamic--pyruvic transaminase 2 (GPT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023790.1

ORF Insert Sequence:

ATGCGAATGG GGTCCTACAG TGCTAGCCAG GGTGTCAACT GCATCCGTGA AGATGTGGCT 
GCCTACATCA CCAGGAGGGA TGGCGGTGTG CCTGCGGACC CCGACAACAT CTACCTGACC 
ACGGGAGCTA GTGACGGCAT TTCTACGATC CTGAAGATCC TCGTCTCCGG GGGCGGCAAG 
TCACGGACAG GTGTGATGAT CCCCATCCCA CAATATCCCC TCTATTCAGC TGTCATCTCT 
GAGCTCGACG CCATCCAGGT GAATTACTAC CTGGACGAGG AGAACTGCTG GGCGCTGAAT 
GTGAATGAGC TCCGGCGGGC GGTGCAGGAG GCCAAAGACC ACTGTGATCC TAAGGTGCTC 
TGCATAATCA ACCCTGGGAA CCCCACAGGC CAGGTACAAA GCAGAAAGTG CATAGAAGAT 
GTGATCCACT TTGCCTGGGA AGAGAAGCTC TTTCTCCTGG CTGATGAGGT GTACCAGGAC 
AACGTGTACT CTCCAGATTG CAGATTCCAC TCCTTCAAGA AGGTGCTGTA CGAGATGGGG 
CCCGAGTACT CCAGCAACGT GGAGCTCGCC TCCTTCCACT CCACCTCCAA GGGCTACATG 
GGCGAGTGTG GTTACAGAGG AGGCTACATG GAGGTGATCA ACCTGCACCC TGAGATCAAG 
GGCCAGCTGG TGAAGCTGCT GTCGGTGCGC CTGTGCCCCC CAGTGTCTGG GCAGGCCGCC 
ATGGACATTG TCGTGAACCC CCCGGTGGCA GGAGAGGAGT CCTTTGAGCA ATTCAGCCGA 
GAGAAGGAGT CGGTCCTGGG TAATCTGGCC AAAAAAGCAA AGCTGACGGA AGACCTGTTT 
AACCAAGTCC CAGGAATTCA CTGCAACCCC TTGCAGGGGG CCATGTACGC CTTCCCTCGG 
ATCTTCATTC CTGCCAAAGC TGTGGAGGCT GCTCAGGCCC ATCAAATGGC TCCAGACATG 
TTCTACTGCA TGAAGCTCCT GGAGGAGACT GGCATCTGTG TCGTGCCCGG CAGTGGCTTT 
GGGCAGAGGG AAGGCACTTA CCACTTCAGG ATGACTATCC TCCCTCCAGT GGAGAAGCTG 
AAAACGGTGC TGCAGAAGGT GAAAGACTTC CACATCAACT TCCTGGAGAA GTACGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel compound heterozygous mutations in GPT2 linked to microcephaly, and intellectual developmental disability with or without spastic paraplegia.
American journal of medical genetics. Part A176(2)421-425(2018 Feb)
Kaymakcalan H,Yarman Y,Goc N,Toy F,Meral C,Ercan-Sencicek AG,Gunel M

Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.
Theranostics7(12)3021-3033(2017)
Cao Y,Lin SH,Wang Y,Chin YE,Kang L,Mi J

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GPT2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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