GTPBP3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GTPBP3
Entrez Gene ID	84705
Full Name	GTP binding protein 3, mitochondrial
Synonyms	COXPD23,GTPBG3,MSS1,MTGP1,THDF1
General protein information	Preferred Names GTP binding protein 3, mitochondrial Names tRNA modification GTPase GTPBP3, mitochondrial mitochondrial GTP-binding protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.11
Summary	This locus encodes a GTP-binding protein. The encoded protein is localized to the mitochondria and may play a role in mitochondrial tRNA modification. Polymorphisms at this locus may be associated with severity of aminoglycoside-induced deafness, a disease associated with a mutation in the 12S rRNA. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Sep 2010].
Disorder MIM:	608536
Disorder Html:	Combined oxidative phosphorylation deficiency 23, 616198 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001195422.1	NP_001182351.1	tRNA modification GTPase GTPBP3, mitochondrial isoform VII
NM_032620.4	NP_116009.2	tRNA modification GTPase GTPBP3, mitochondrial isoform V
NM_133644.3	NP_598399.2	tRNA modification GTPase GTPBP3, mitochondrial isoform IV
NM_032620.3	NP_116009.2	tRNA modification GTPase GTPBP3, mitochondrial isoform V
NM_133644.4	NP_598399.2	tRNA modification GTPase GTPBP3, mitochondrial isoform IV
NM_001128855.2	NP_001122327.1	tRNA modification GTPase GTPBP3, mitochondrial isoform III
NM_001128855.3	NP_001122327.1	tRNA modification GTPase GTPBP3, mitochondrial isoform III

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MSS1	NP_013736.1
Pan troglodytes (chimpanzee)	LOC748414	XP_001161204.3
Canis lupus familiaris (dog)	GTPBP3	XP_005632781.1
Mus musculus (house mouse)	Gtpbp3	NP_115933.2
Rattus norvegicus (Norway rat)	Gtpbp3	NP_001011919.1
Gallus gallus (chicken)	LOC100857447	XP_003643536.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002133	XP_320903.3
Schizosaccharomyces pombe (fission yeast)	mss1	NP_593144.1
Bos taurus (cattle)	GTPBP3	NP_001193147.1
Danio rerio (zebrafish)	gtpbp3	NP_001018605.1
Drosophila melanogaster (fruit fly)	CG18528	NP_651251.3
Kluyveromyces lactis	KLLA0E07657g	XP_454294.1
Eremothecium gossypii	AGOS_ABR063C	NP_983009.3
Magnaporthe oryzae (rice blast fungus)	MGG_04070	XP_003719769.1
Xenopus tropicalis (tropical clawed frog)	gtpbp3	XP_004911076.1
Homo sapiens (human)	GTPBP3	NP_598399.2
Caenorhabditis elegans (roundworm)	F39B2.7	NP_493573.1
Neurospora crassa	NCU04149	XP_961305.1
Arabidopsis thaliana (thale cress)	AT1G78010	NP_177924.3

Gene Ontology
Bibliography

Related articles in PubMed

Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPAR?-UCP2-AMPK axis.
Boutoual R, Meseguer S, Villarroya M, Mart?n-Hern?ndez E, Errami M, Mart?n MA, Casado M, Armengod ME
Scientific reports8(1)1163(2018 Jan)

microRNA-mediated differential expression of TRMU, GTPBP3 and MTO1 in cell models of mitochondrial-DNA diseases.
Meseguer S, Boix O, Navarro-Gonz?lez C, Villarroya M, Boutoual R, Emperador S, Garc?a-Arum? E, Montoya J, Armengod ME
Scientific reports7(1)6209(2017 Jul)

Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier.
Mart?nez-Zamora A, Meseguer S, Esteve JM, Villarroya M, Aguado C, Enr?quez JA, Knecht E, Armengod ME
PloS one10(12)e0144273(2015)

Mutations in GTPBP3 cause a mitochondrial translation defect associated with hypertrophic cardiomyopathy, lactic acidosis, and encephalopathy.
Kopajtich R, Nicholls TJ, Rorbach J, Metodiev MD, Freisinger P, Mandel H, Vanlander A, Ghezzi D, Carrozzo R, Taylor RW, Marquard K, Murayama K, Wieland T, Schwarzmayr T, Mayr JA, Pearce SF, Powell CA, Saada A, Ohtake A, Invernizzi F, Lamantea E, Sommerville EW, Pyle A, Chinnery PF, Crushell E, Okazaki Y, Kohda M, Kishita Y, Tokuzawa Y, Assouline Z, Rio M, Feillet F, Mousson de Camaret B, Chretien D, Munnich A, Menten B, Sante T, Smet J, R?gal L, Lorber A, Khoury A, Zeviani M, Strom TM, Meitinger T, Bertini ES, Van Coster R, Klopstock T, R?tig A, Haack TB, Minczuk M, Prokisch H
American journal of human genetics95(6)708-20(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that post-transcriptional expression of GTPBP3, MTO1 and TRMU genes is down-regulated, leading to mt-tRNA hypomodification and contributing to mitochondrial dysfunction in MELAS cybrids.
Title: The ROS-sensitive microRNA-9/9* controls the expression of mitochondrial tRNA-modifying enzymes and is involved in the molecular mechanism of MELAS syndrome.

GTPBP3 defective expression is associated with an mitochondrial-tRNA hypomodification status.GTPBP3 plays a role in the regulation of UCP2 and MCP1 proteins through AMPK signaling.
Title: Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier.

Most individuals with GTPBP3 mutations developed neurological symptoms and MRI involvement of thalamus, putamen, and brainstem resembling Leigh syndrome.
Title: Mutations in GTPBP3 cause a mitochondrial translation defect associated with hypertrophic cardiomyopathy, lactic acidosis, and encephalopathy.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Meta-analysis of gene-disease association. (HuGE Navigator)
Title: Genetic association analysis of 13 nuclear-encoded mitochondrial candidate genes with type II diabetes mellitus: the DAMAGE study.

The following GTPBP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTPBP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09374	NM_032620.4 Latest version!	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant V, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu09444	NM_001128855.3 Latest version!	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant III, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu09456	NM_133644.4 Latest version!	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant IV, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu18549	NM_001195422.1 Latest version!	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant VII, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu09374	NM_032620.3	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant V, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu09456	NM_133644.3	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant IV, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu09444	NM_001128855.2	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant III, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GTPBP3 ( NM_032620.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_116009.2 Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant V, mRNA.

Price & Availability↑

CloneID	OHu09374
Clone ID Related Accession (Same CDS sequence)	NM_032620.4 , NM_032620.3
Accession Version	NM_032620.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform V
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA044241.1, BC019261.1 and AY078988.1. On Nov 23, 2018 this sequence version replaced NM_032620.3. Transcript Variant: This variant (V) lacks an in-frame segment in the CDS, compared to variant IV. The resulting protein (isoform V) is shorter than isoform IV. This variant is also referred to as isoform I in PubMed IDs 12370316 and 18852288. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC019261.1, AK027606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000324894.13/ ENSP00000313818.7 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGCCGACA TCGAAGTACG GGCACTGCAG GTGGCCCTGG GTGCACATCT ACGAGATGCC 
AGGCGCGGGC AGAGGCTCCG CTCAGGGGTG CACGTAGTGG TCACTGGACC CCCCAATGCG 
GGCAAGAGCA GCCTAGTGAA CCTGCTCAGT CGGAAGCCTG TGTCCATCGT GTCCCCGGAG 
CCAGGGACCA CCCGTGACGT GCTGGAGACC CCAGTCGACC TGGCCGGATT TCCTGTGCTG 
CTGAGCGACA CGGCTGGGTT GCGGGAGGGC GTGGGGCCCG TGGAGCAGGA GGGCGTGCGG 
CGCGCCCGGG AGAGGCTAGA GCAGGCTGAC CTCATTCTGG CCATGCTGGA TGCTTCTGAC 
CTGGCCTCTC CCTCCAGTTG CAACTTCCTG GCCACCGTCG TAGCCTCTGT GGGAGCCCAG 
AGCCCCAGTG ACAGCAGCCA GCGCCTCCTC CTGGTGCTGA ACAAGTCGGA CCTGCTGTCC 
CCGGAGGGCC CAGGTCCCGG TCCTGACCTG CCCCCGCACC TGCTGCTGTC CTGTCTGACG 
GGAGAGGGGC TGGACGGCCT CCTGGAGGCG CTGAGGAAGG AGCTAGCTGC AGTGTGTGGG 
GACCCGTCCA CAGATCCCCC GCTGCTGACC CGAGCAAGGC ACCAGCACCA CCTCCAGGGT 
TGCCTGGATG CCCTCGGCCA CTACAAGCAG TCAAAAGACC TGGCCCTGGC GGCAGAGGCG 
CTGCGGGTGG CCCGGGGTCA CCTGACCCGG CTCACAGGTG GAGGGGGTAC CGAGGAGATC 
CTGGACATCA TCTTCCAGGA CTTCTGTGTG GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116009.2
CDS	60..1538
Translation	MWRGLWTLAAQAARGPRRLCTRRSSGAPAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALRILTAPRDLPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFANGKLNLTEVEGLADLIHAETEAQRRQALRQLDGELGHLCRGWAETLTKALAHVEAYIDFGEDDNLEEGVLEQADIEVRALQVALGAHLRDARRGQRLRSGVHVVVTGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPVLLSDTAGLREGVGPVEQEGVRRARERLEQADLILAMLDASDLASPSSCNFLATVVASVGAQSPSDSSQRLLLVLNKSDLLSPEGPGPGPDLPPHLLLSCLTGEGLDGLLEALRKELAAVCGDPSTDPPLLTRARHQHHLQGCLDALGHYKQSKDLALAAEALRVARGHLTRLTGGGGTEEILDIIFQDFCVGK

Target ORF information:

RefSeq Version	NM_032620.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant V, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032620.4

ORF Insert Sequence:

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGCCGACA TCGAAGTACG GGCACTGCAG GTGGCCCTGG GTGCACATCT ACGAGATGCC 
AGGCGCGGGC AGAGGCTCCG CTCAGGGGTG CACGTAGTGG TCACTGGACC CCCCAATGCG 
GGCAAGAGCA GCCTAGTGAA CCTGCTCAGT CGGAAGCCTG TGTCCATCGT GTCCCCGGAG 
CCAGGGACCA CCCGTGACGT GCTGGAGACC CCAGTCGACC TGGCCGGATT TCCTGTGCTG 
CTGAGCGACA CGGCTGGGTT GCGGGAGGGC GTGGGGCCCG TGGAGCAGGA GGGCGTGCGG 
CGCGCCCGGG AGAGGCTAGA GCAGGCTGAC CTCATTCTGG CCATGCTGGA TGCTTCTGAC 
CTGGCCTCTC CCTCCAGTTG CAACTTCCTG GCCACCGTCG TAGCCTCTGT GGGAGCCCAG 
AGCCCCAGTG ACAGCAGCCA GCGCCTCCTC CTGGTGCTGA ACAAGTCGGA CCTGCTGTCC 
CCGGAGGGCC CAGGTCCCGG TCCTGACCTG CCCCCGCACC TGCTGCTGTC CTGTCTGACG 
GGAGAGGGGC TGGACGGCCT CCTGGAGGCG CTGAGGAAGG AGCTAGCTGC AGTGTGTGGG 
GACCCGTCCA CAGATCCCCC GCTGCTGACC CGAGCAAGGC ACCAGCACCA CCTCCAGGGT 
TGCCTGGATG CCCTCGGCCA CTACAAGCAG TCAAAAGACC TGGCCCTGGC GGCAGAGGCG 
CTGCGGGTGG CCCGGGGTCA CCTGACCCGG CTCACAGGTG GAGGGGGTAC CGAGGAGATC 
CTGGACATCA TCTTCCAGGA CTTCTGTGTG GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( NM_032620.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116009.2 Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant V, mRNA.

Price & Availability↑

CloneID	OHu09374
Clone ID Related Accession (Same CDS sequence)	NM_032620.4 , NM_032620.3
Accession Version	NM_032620.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform V
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA044241.1, BC019261.1 and AY078988.1. On Sep 10, 2010 this sequence version replaced NM_032620.2. Transcript Variant: This variant (V) lacks an in-frame segment in the CDS, compared to variant IV. The resulting protein (isoform V) is shorter than isoform IV. This variant is also referred to as isoform I in PubMed IDs 12370316 and 18852288. ##Evidence-Data-START## Transcript exon combination :: BC019261.1, AK027606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGCCGACA TCGAAGTACG GGCACTGCAG GTGGCCCTGG GTGCACATCT ACGAGATGCC 
AGGCGCGGGC AGAGGCTCCG CTCAGGGGTG CACGTAGTGG TCACTGGACC CCCCAATGCG 
GGCAAGAGCA GCCTAGTGAA CCTGCTCAGT CGGAAGCCTG TGTCCATCGT GTCCCCGGAG 
CCAGGGACCA CCCGTGACGT GCTGGAGACC CCAGTCGACC TGGCCGGATT TCCTGTGCTG 
CTGAGCGACA CGGCTGGGTT GCGGGAGGGC GTGGGGCCCG TGGAGCAGGA GGGCGTGCGG 
CGCGCCCGGG AGAGGCTAGA GCAGGCTGAC CTCATTCTGG CCATGCTGGA TGCTTCTGAC 
CTGGCCTCTC CCTCCAGTTG CAACTTCCTG GCCACCGTCG TAGCCTCTGT GGGAGCCCAG 
AGCCCCAGTG ACAGCAGCCA GCGCCTCCTC CTGGTGCTGA ACAAGTCGGA CCTGCTGTCC 
CCGGAGGGCC CAGGTCCCGG TCCTGACCTG CCCCCGCACC TGCTGCTGTC CTGTCTGACG 
GGAGAGGGGC TGGACGGCCT CCTGGAGGCG CTGAGGAAGG AGCTAGCTGC AGTGTGTGGG 
GACCCGTCCA CAGATCCCCC GCTGCTGACC CGAGCAAGGC ACCAGCACCA CCTCCAGGGT 
TGCCTGGATG CCCTCGGCCA CTACAAGCAG TCAAAAGACC TGGCCCTGGC GGCAGAGGCG 
CTGCGGGTGG CCCGGGGTCA CCTGACCCGG CTCACAGGTG GAGGGGGTAC CGAGGAGATC 
CTGGACATCA TCTTCCAGGA CTTCTGTGTG GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116009.2
CDS	96..1574
Translation	MWRGLWTLAAQAARGPRRLCTRRSSGAPAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALRILTAPRDLPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFANGKLNLTEVEGLADLIHAETEAQRRQALRQLDGELGHLCRGWAETLTKALAHVEAYIDFGEDDNLEEGVLEQADIEVRALQVALGAHLRDARRGQRLRSGVHVVVTGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPVLLSDTAGLREGVGPVEQEGVRRARERLEQADLILAMLDASDLASPSSCNFLATVVASVGAQSPSDSSQRLLLVLNKSDLLSPEGPGPGPDLPPHLLLSCLTGEGLDGLLEALRKELAAVCGDPSTDPPLLTRARHQHHLQGCLDALGHYKQSKDLALAAEALRVARGHLTRLTGGGGTEEILDIIFQDFCVGK

Target ORF information:

RefSeq Version	NM_032620.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant V, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032620.3

ORF Insert Sequence:

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGCCGACA TCGAAGTACG GGCACTGCAG GTGGCCCTGG GTGCACATCT ACGAGATGCC 
AGGCGCGGGC AGAGGCTCCG CTCAGGGGTG CACGTAGTGG TCACTGGACC CCCCAATGCG 
GGCAAGAGCA GCCTAGTGAA CCTGCTCAGT CGGAAGCCTG TGTCCATCGT GTCCCCGGAG 
CCAGGGACCA CCCGTGACGT GCTGGAGACC CCAGTCGACC TGGCCGGATT TCCTGTGCTG 
CTGAGCGACA CGGCTGGGTT GCGGGAGGGC GTGGGGCCCG TGGAGCAGGA GGGCGTGCGG 
CGCGCCCGGG AGAGGCTAGA GCAGGCTGAC CTCATTCTGG CCATGCTGGA TGCTTCTGAC 
CTGGCCTCTC CCTCCAGTTG CAACTTCCTG GCCACCGTCG TAGCCTCTGT GGGAGCCCAG 
AGCCCCAGTG ACAGCAGCCA GCGCCTCCTC CTGGTGCTGA ACAAGTCGGA CCTGCTGTCC 
CCGGAGGGCC CAGGTCCCGG TCCTGACCTG CCCCCGCACC TGCTGCTGTC CTGTCTGACG 
GGAGAGGGGC TGGACGGCCT CCTGGAGGCG CTGAGGAAGG AGCTAGCTGC AGTGTGTGGG 
GACCCGTCCA CAGATCCCCC GCTGCTGACC CGAGCAAGGC ACCAGCACCA CCTCCAGGGT 
TGCCTGGATG CCCTCGGCCA CTACAAGCAG TCAAAAGACC TGGCCCTGGC GGCAGAGGCG 
CTGCGGGTGG CCCGGGGTCA CCTGACCCGG CTCACAGGTG GAGGGGGTAC CGAGGAGATC 
CTGGACATCA TCTTCCAGGA CTTCTGTGTG GGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( NM_001128855.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001122327.1 Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant III, mRNA.

Price & Availability↑

CloneID	OHu09444
Clone ID Related Accession (Same CDS sequence)	NM_001128855.2 , NM_001128855.3
Accession Version	NM_001128855.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform III
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA044241.1, AF360743.1, AK298485.1 and BC019261.1. On Sep 10, 2010 this sequence version replaced NM_001128855.1. Transcript Variant: This variant (III) lacks an in-frame segment and uses an alternate, in-frame splice site in the CDS, compared to variant IV. The resulting protein (isoform III) is shorter than isoform IV. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF360743.1, AF360744.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGCCGACA TCGAAGTACG GGCACTGCAG GTGGCCCTGG GTGCACATCT ACGAGATGCC 
AGGCGCGGGC AGAGGCTCCG CTCAGGGGTG CACGTAGTGG TCACTGGACC CCCCAATGCG 
GGCAAGAGCA GCCTAGTGAA CCTGCTCAGT CGGAAGCCTG TGTCCATCGT GTCCCCGGAG 
CCAGGGACCA CCCGTGACGT GCTGGAGACC CCAGTCGACC TGGCCGGATT TCCTGTGCTG 
CTGAGCGACA CGGCTGGGTT GCGGGAGGGC GTGGGGCCCG TGGAGCAGGA GGGCGTGCGG 
CGCGCCCGGG AGAGTTGCAA CTTCCTGGCC ACCGTCGTAG CCTCTGTGGG AGCCCAGAGC 
CCCAGTGACA GCAGCCAGCG CCTCCTCCTG GTGCTGAACA AGTCGGACCT GCTGTCCCCG 
GAGGGCCCAG GTCCCGGTCC TGACCTGCCC CCGCACCTGC TGCTGTCCTG TCTGACGGGA 
GAGGGGCTGG ACGGCCTCCT GGAGGCGCTG AGGAAGGAGC TAGCTGCAGT GTGTGGGGAC 
CCGTCCACAG ATCCCCCGCT GCTGACCCGA GCAAGGCACC AGCACCACCT CCAGGGTTGC 
CTGGATGCCC TCGGCCACTA CAAGCAGTCA AAAGACCTGG CCCTGGCGGC AGAGGCGCTG 
CGGGTGGCCC GGGGTCACCT GACCCGGCTC ACAGGTGGAG GGGGTACCGA GGAGATCCTG 
GACATCATCT TCCAGGACTT CTGTGTGGGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122327.1
CDS	96..1511
Translation	MWRGLWTLAAQAARGPRRLCTRRSSGAPAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALRILTAPRDLPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFANGKLNLTEVEGLADLIHAETEAQRRQALRQLDGELGHLCRGWAETLTKALAHVEAYIDFGEDDNLEEGVLEQADIEVRALQVALGAHLRDARRGQRLRSGVHVVVTGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPVLLSDTAGLREGVGPVEQEGVRRARESCNFLATVVASVGAQSPSDSSQRLLLVLNKSDLLSPEGPGPGPDLPPHLLLSCLTGEGLDGLLEALRKELAAVCGDPSTDPPLLTRARHQHHLQGCLDALGHYKQSKDLALAAEALRVARGHLTRLTGGGGTEEILDIIFQDFCVGK

Target ORF information:

RefSeq Version	NM_001128855.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant III, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128855.2

ORF Insert Sequence:

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGCCGACA TCGAAGTACG GGCACTGCAG GTGGCCCTGG GTGCACATCT ACGAGATGCC 
AGGCGCGGGC AGAGGCTCCG CTCAGGGGTG CACGTAGTGG TCACTGGACC CCCCAATGCG 
GGCAAGAGCA GCCTAGTGAA CCTGCTCAGT CGGAAGCCTG TGTCCATCGT GTCCCCGGAG 
CCAGGGACCA CCCGTGACGT GCTGGAGACC CCAGTCGACC TGGCCGGATT TCCTGTGCTG 
CTGAGCGACA CGGCTGGGTT GCGGGAGGGC GTGGGGCCCG TGGAGCAGGA GGGCGTGCGG 
CGCGCCCGGG AGAGTTGCAA CTTCCTGGCC ACCGTCGTAG CCTCTGTGGG AGCCCAGAGC 
CCCAGTGACA GCAGCCAGCG CCTCCTCCTG GTGCTGAACA AGTCGGACCT GCTGTCCCCG 
GAGGGCCCAG GTCCCGGTCC TGACCTGCCC CCGCACCTGC TGCTGTCCTG TCTGACGGGA 
GAGGGGCTGG ACGGCCTCCT GGAGGCGCTG AGGAAGGAGC TAGCTGCAGT GTGTGGGGAC 
CCGTCCACAG ATCCCCCGCT GCTGACCCGA GCAAGGCACC AGCACCACCT CCAGGGTTGC 
CTGGATGCCC TCGGCCACTA CAAGCAGTCA AAAGACCTGG CCCTGGCGGC AGAGGCGCTG 
CGGGTGGCCC GGGGTCACCT GACCCGGCTC ACAGGTGGAG GGGGTACCGA GGAGATCCTG 
GACATCATCT TCCAGGACTT CTGTGTGGGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( NM_001128855.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001122327.1 Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant III, mRNA.

Price & Availability↑

CloneID	OHu09444
Clone ID Related Accession (Same CDS sequence)	NM_001128855.2 , NM_001128855.3
Accession Version	NM_001128855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform III
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA044241.1, AF360743.1, AK298485.1 and BC019261.1. On Jun 1, 2019 this sequence version replaced NM_001128855.2. Transcript Variant: This variant (III) lacks an in-frame segment and uses an alternate, in-frame splice site in the CDS, compared to variant IV. The resulting protein (isoform III) is shorter than isoform IV. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.889655.1, AF360743.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGCCGACA TCGAAGTACG GGCACTGCAG GTGGCCCTGG GTGCACATCT ACGAGATGCC 
AGGCGCGGGC AGAGGCTCCG CTCAGGGGTG CACGTAGTGG TCACTGGACC CCCCAATGCG 
GGCAAGAGCA GCCTAGTGAA CCTGCTCAGT CGGAAGCCTG TGTCCATCGT GTCCCCGGAG 
CCAGGGACCA CCCGTGACGT GCTGGAGACC CCAGTCGACC TGGCCGGATT TCCTGTGCTG 
CTGAGCGACA CGGCTGGGTT GCGGGAGGGC GTGGGGCCCG TGGAGCAGGA GGGCGTGCGG 
CGCGCCCGGG AGAGTTGCAA CTTCCTGGCC ACCGTCGTAG CCTCTGTGGG AGCCCAGAGC 
CCCAGTGACA GCAGCCAGCG CCTCCTCCTG GTGCTGAACA AGTCGGACCT GCTGTCCCCG 
GAGGGCCCAG GTCCCGGTCC TGACCTGCCC CCGCACCTGC TGCTGTCCTG TCTGACGGGA 
GAGGGGCTGG ACGGCCTCCT GGAGGCGCTG AGGAAGGAGC TAGCTGCAGT GTGTGGGGAC 
CCGTCCACAG ATCCCCCGCT GCTGACCCGA GCAAGGCACC AGCACCACCT CCAGGGTTGC 
CTGGATGCCC TCGGCCACTA CAAGCAGTCA AAAGACCTGG CCCTGGCGGC AGAGGCGCTG 
CGGGTGGCCC GGGGTCACCT GACCCGGCTC ACAGGTGGAG GGGGTACCGA GGAGATCCTG 
GACATCATCT TCCAGGACTT CTGTGTGGGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122327.1
CDS	60..1475
Translation	MWRGLWTLAAQAARGPRRLCTRRSSGAPAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALRILTAPRDLPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFANGKLNLTEVEGLADLIHAETEAQRRQALRQLDGELGHLCRGWAETLTKALAHVEAYIDFGEDDNLEEGVLEQADIEVRALQVALGAHLRDARRGQRLRSGVHVVVTGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPVLLSDTAGLREGVGPVEQEGVRRARESCNFLATVVASVGAQSPSDSSQRLLLVLNKSDLLSPEGPGPGPDLPPHLLLSCLTGEGLDGLLEALRKELAAVCGDPSTDPPLLTRARHQHHLQGCLDALGHYKQSKDLALAAEALRVARGHLTRLTGGGGTEEILDIIFQDFCVGK

Target ORF information:

RefSeq Version	NM_001128855.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant III, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128855.3

ORF Insert Sequence:

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGCCGACA TCGAAGTACG GGCACTGCAG GTGGCCCTGG GTGCACATCT ACGAGATGCC 
AGGCGCGGGC AGAGGCTCCG CTCAGGGGTG CACGTAGTGG TCACTGGACC CCCCAATGCG 
GGCAAGAGCA GCCTAGTGAA CCTGCTCAGT CGGAAGCCTG TGTCCATCGT GTCCCCGGAG 
CCAGGGACCA CCCGTGACGT GCTGGAGACC CCAGTCGACC TGGCCGGATT TCCTGTGCTG 
CTGAGCGACA CGGCTGGGTT GCGGGAGGGC GTGGGGCCCG TGGAGCAGGA GGGCGTGCGG 
CGCGCCCGGG AGAGTTGCAA CTTCCTGGCC ACCGTCGTAG CCTCTGTGGG AGCCCAGAGC 
CCCAGTGACA GCAGCCAGCG CCTCCTCCTG GTGCTGAACA AGTCGGACCT GCTGTCCCCG 
GAGGGCCCAG GTCCCGGTCC TGACCTGCCC CCGCACCTGC TGCTGTCCTG TCTGACGGGA 
GAGGGGCTGG ACGGCCTCCT GGAGGCGCTG AGGAAGGAGC TAGCTGCAGT GTGTGGGGAC 
CCGTCCACAG ATCCCCCGCT GCTGACCCGA GCAAGGCACC AGCACCACCT CCAGGGTTGC 
CTGGATGCCC TCGGCCACTA CAAGCAGTCA AAAGACCTGG CCCTGGCGGC AGAGGCGCTG 
CGGGTGGCCC GGGGTCACCT GACCCGGCTC ACAGGTGGAG GGGGTACCGA GGAGATCCTG 
GACATCATCT TCCAGGACTT CTGTGTGGGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( NM_133644.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_598399.2 Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant IV, mRNA.

Price & Availability↑

CloneID	OHu09456
Clone ID Related Accession (Same CDS sequence)	NM_133644.3 , NM_133644.4
Accession Version	NM_133644.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform IV
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA044241.1, AK298485.1, AY078987.1 and BC019261.1. On Sep 10, 2010 this sequence version replaced NM_133644.2. Transcript Variant: This variant (IV) represents the longest transcript and it encodes the longest protein (isoform IV). This variant is also referred to as isoform V in PubMed IDs 12370316 and 18852288. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY078987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGGTGGGT CTACCTGGTG GTGGGGGAGG AAGACACCTC ATATCAGCCC TCAAAGGCTC 
CCCTCACTGT CTCTCTCTGC CTGCCTTCTC TCACCCACAG CCGACATCGA AGTACGGGCA 
CTGCAGGTGG CCCTGGGTGC ACATCTACGA GATGCCAGGC GCGGGCAGAG GCTCCGCTCA 
GGGGTGCACG TAGTGGTCAC TGGACCCCCC AATGCGGGCA AGAGCAGCCT AGTGAACCTG 
CTCAGTCGGA AGCCTGTGTC CATCGTGTCC CCGGAGCCAG GGACCACCCG TGACGTGCTG 
GAGACCCCAG TCGACCTGGC CGGATTTCCT GTGCTGCTGA GCGACACGGC TGGGTTGCGG 
GAGGGCGTGG GGCCCGTGGA GCAGGAGGGC GTGCGGCGCG CCCGGGAGAG GCTAGAGCAG 
GCTGACCTCA TTCTGGCCAT GCTGGATGCT TCTGACCTGG CCTCTCCCTC CAGTTGCAAC 
TTCCTGGCCA CCGTCGTAGC CTCTGTGGGA GCCCAGAGCC CCAGTGACAG CAGCCAGCGC 
CTCCTCCTGG TGCTGAACAA GTCGGACCTG CTGTCCCCGG AGGGCCCAGG TCCCGGTCCT 
GACCTGCCCC CGCACCTGCT GCTGTCCTGT CTGACGGGAG AGGGGCTGGA CGGCCTCCTG 
GAGGCGCTGA GGAAGGAGCT AGCTGCAGTG TGTGGGGACC CGTCCACAGA TCCCCCGCTG 
CTGACCCGAG CAAGGCACCA GCACCACCTC CAGGGTTGCC TGGATGCCCT CGGCCACTAC 
AAGCAGTCAA AAGACCTGGC CCTGGCGGCA GAGGCGCTGC GGGTGGCCCG GGGTCACCTG 
ACCCGGCTCA CAGGTGGAGG GGGTACCGAG GAGATCCTGG ACATCATCTT CCAGGACTTC 
TGTGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598399.2
CDS	96..1670
Translation	MWRGLWTLAAQAARGPRRLCTRRSSGAPAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALRILTAPRDLPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFANGKLNLTEVEGLADLIHAETEAQRRQALRQLDGELGHLCRGWAETLTKALAHVEAYIDFGEDDNLEEGVLEQGGSTWWWGRKTPHISPQRLPSLSLSACLLSPTADIEVRALQVALGAHLRDARRGQRLRSGVHVVVTGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPVLLSDTAGLREGVGPVEQEGVRRARERLEQADLILAMLDASDLASPSSCNFLATVVASVGAQSPSDSSQRLLLVLNKSDLLSPEGPGPGPDLPPHLLLSCLTGEGLDGLLEALRKELAAVCGDPSTDPPLLTRARHQHHLQGCLDALGHYKQSKDLALAAEALRVARGHLTRLTGGGGTEEILDIIFQDFCVGK

Target ORF information:

RefSeq Version	NM_133644.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant IV, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133644.3

ORF Insert Sequence:

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGGTGGGT CTACCTGGTG GTGGGGGAGG AAGACACCTC ATATCAGCCC TCAAAGGCTC 
CCCTCACTGT CTCTCTCTGC CTGCCTTCTC TCACCCACAG CCGACATCGA AGTACGGGCA 
CTGCAGGTGG CCCTGGGTGC ACATCTACGA GATGCCAGGC GCGGGCAGAG GCTCCGCTCA 
GGGGTGCACG TAGTGGTCAC TGGACCCCCC AATGCGGGCA AGAGCAGCCT AGTGAACCTG 
CTCAGTCGGA AGCCTGTGTC CATCGTGTCC CCGGAGCCAG GGACCACCCG TGACGTGCTG 
GAGACCCCAG TCGACCTGGC CGGATTTCCT GTGCTGCTGA GCGACACGGC TGGGTTGCGG 
GAGGGCGTGG GGCCCGTGGA GCAGGAGGGC GTGCGGCGCG CCCGGGAGAG GCTAGAGCAG 
GCTGACCTCA TTCTGGCCAT GCTGGATGCT TCTGACCTGG CCTCTCCCTC CAGTTGCAAC 
TTCCTGGCCA CCGTCGTAGC CTCTGTGGGA GCCCAGAGCC CCAGTGACAG CAGCCAGCGC 
CTCCTCCTGG TGCTGAACAA GTCGGACCTG CTGTCCCCGG AGGGCCCAGG TCCCGGTCCT 
GACCTGCCCC CGCACCTGCT GCTGTCCTGT CTGACGGGAG AGGGGCTGGA CGGCCTCCTG 
GAGGCGCTGA GGAAGGAGCT AGCTGCAGTG TGTGGGGACC CGTCCACAGA TCCCCCGCTG 
CTGACCCGAG CAAGGCACCA GCACCACCTC CAGGGTTGCC TGGATGCCCT CGGCCACTAC 
AAGCAGTCAA AAGACCTGGC CCTGGCGGCA GAGGCGCTGC GGGTGGCCCG GGGTCACCTG 
ACCCGGCTCA CAGGTGGAGG GGGTACCGAG GAGATCCTGG ACATCATCTT CCAGGACTTC 
TGTGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( NM_133644.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_598399.2 Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant IV, mRNA.

Price & Availability↑

CloneID	OHu09456
Clone ID Related Accession (Same CDS sequence)	NM_133644.3 , NM_133644.4
Accession Version	NM_133644.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform IV
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA044241.1, AK298485.1, AY078987.1 and BC019261.1. On May 31, 2019 this sequence version replaced NM_133644.3. Transcript Variant: This variant (IV) represents the longest transcript and it encodes the longest protein (isoform IV). This variant is also referred to as isoform V in PubMed IDs 12370316 and 18852288. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1348910.1, AY078987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGGTGGGT CTACCTGGTG GTGGGGGAGG AAGACACCTC ATATCAGCCC TCAAAGGCTC 
CCCTCACTGT CTCTCTCTGC CTGCCTTCTC TCACCCACAG CCGACATCGA AGTACGGGCA 
CTGCAGGTGG CCCTGGGTGC ACATCTACGA GATGCCAGGC GCGGGCAGAG GCTCCGCTCA 
GGGGTGCACG TAGTGGTCAC TGGACCCCCC AATGCGGGCA AGAGCAGCCT AGTGAACCTG 
CTCAGTCGGA AGCCTGTGTC CATCGTGTCC CCGGAGCCAG GGACCACCCG TGACGTGCTG 
GAGACCCCAG TCGACCTGGC CGGATTTCCT GTGCTGCTGA GCGACACGGC TGGGTTGCGG 
GAGGGCGTGG GGCCCGTGGA GCAGGAGGGC GTGCGGCGCG CCCGGGAGAG GCTAGAGCAG 
GCTGACCTCA TTCTGGCCAT GCTGGATGCT TCTGACCTGG CCTCTCCCTC CAGTTGCAAC 
TTCCTGGCCA CCGTCGTAGC CTCTGTGGGA GCCCAGAGCC CCAGTGACAG CAGCCAGCGC 
CTCCTCCTGG TGCTGAACAA GTCGGACCTG CTGTCCCCGG AGGGCCCAGG TCCCGGTCCT 
GACCTGCCCC CGCACCTGCT GCTGTCCTGT CTGACGGGAG AGGGGCTGGA CGGCCTCCTG 
GAGGCGCTGA GGAAGGAGCT AGCTGCAGTG TGTGGGGACC CGTCCACAGA TCCCCCGCTG 
CTGACCCGAG CAAGGCACCA GCACCACCTC CAGGGTTGCC TGGATGCCCT CGGCCACTAC 
AAGCAGTCAA AAGACCTGGC CCTGGCGGCA GAGGCGCTGC GGGTGGCCCG GGGTCACCTG 
ACCCGGCTCA CAGGTGGAGG GGGTACCGAG GAGATCCTGG ACATCATCTT CCAGGACTTC 
TGTGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598399.2
CDS	60..1634
Translation	MWRGLWTLAAQAARGPRRLCTRRSSGAPAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALRILTAPRDLPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFANGKLNLTEVEGLADLIHAETEAQRRQALRQLDGELGHLCRGWAETLTKALAHVEAYIDFGEDDNLEEGVLEQGGSTWWWGRKTPHISPQRLPSLSLSACLLSPTADIEVRALQVALGAHLRDARRGQRLRSGVHVVVTGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPVLLSDTAGLREGVGPVEQEGVRRARERLEQADLILAMLDASDLASPSSCNFLATVVASVGAQSPSDSSQRLLLVLNKSDLLSPEGPGPGPDLPPHLLLSCLTGEGLDGLLEALRKELAAVCGDPSTDPPLLTRARHQHHLQGCLDALGHYKQSKDLALAAEALRVARGHLTRLTGGGGTEEILDIIFQDFCVGK

Target ORF information:

RefSeq Version	NM_133644.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant IV, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133644.4

ORF Insert Sequence:

ATGTGGCGGG GGCTTTGGAC CCTGGCGGCC CAAGCGGCAC GTGGGCCTCG CAGATTGTGC 
ACGCGCCGGA GCAGCGGCGC ACCAGCCCCC GGCTCCGGCG CCACCATCTT CGCGCTAAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCAGTG ATCCGGACCA GCGGCCCCGC CAGCGGCCAC 
GCCCTCCGAA TTCTCACAGC ACCCCGAGAC CTGCCCCTTG CTCGCCACGC CAGCCTGCGC 
CTGCTCAGCG ATCCCCGCTC CGGGGAGCCT CTGGACCGCG CACTGGTGCT CTGGTTCCCA 
GGTCCCCAGA GTTTCACCGG TGAGGACTGC GTGGAGTTCC ACGTGCATGG AGGCCCGGCA 
GTGGTGAGCG GCGTCCTGCA GGCCTTGGGC AGCGTGCCAG GGCTTCGACC GGCGGAGGCA 
GGCGAGTTCA CCAGACGGGC GTTCGCCAAT GGGAAGCTGA ACCTGACCGA AGTGGAGGGG 
CTGGCGGACC TTATCCACGC GGAAACAGAG GCGCAGCGGC GGCAGGCCCT CAGGCAGCTG 
GACGGAGAGC TGGGCCACCT CTGCCGTGGC TGGGCCGAGA CCCTCACCAA AGCTCTGGCC 
CACGTGGAGG CCTATATCGA TTTCGGCGAG GATGACAACC TGGAGGAGGG GGTCCTGGAG 
CAAGGTGGGT CTACCTGGTG GTGGGGGAGG AAGACACCTC ATATCAGCCC TCAAAGGCTC 
CCCTCACTGT CTCTCTCTGC CTGCCTTCTC TCACCCACAG CCGACATCGA AGTACGGGCA 
CTGCAGGTGG CCCTGGGTGC ACATCTACGA GATGCCAGGC GCGGGCAGAG GCTCCGCTCA 
GGGGTGCACG TAGTGGTCAC TGGACCCCCC AATGCGGGCA AGAGCAGCCT AGTGAACCTG 
CTCAGTCGGA AGCCTGTGTC CATCGTGTCC CCGGAGCCAG GGACCACCCG TGACGTGCTG 
GAGACCCCAG TCGACCTGGC CGGATTTCCT GTGCTGCTGA GCGACACGGC TGGGTTGCGG 
GAGGGCGTGG GGCCCGTGGA GCAGGAGGGC GTGCGGCGCG CCCGGGAGAG GCTAGAGCAG 
GCTGACCTCA TTCTGGCCAT GCTGGATGCT TCTGACCTGG CCTCTCCCTC CAGTTGCAAC 
TTCCTGGCCA CCGTCGTAGC CTCTGTGGGA GCCCAGAGCC CCAGTGACAG CAGCCAGCGC 
CTCCTCCTGG TGCTGAACAA GTCGGACCTG CTGTCCCCGG AGGGCCCAGG TCCCGGTCCT 
GACCTGCCCC CGCACCTGCT GCTGTCCTGT CTGACGGGAG AGGGGCTGGA CGGCCTCCTG 
GAGGCGCTGA GGAAGGAGCT AGCTGCAGTG TGTGGGGACC CGTCCACAGA TCCCCCGCTG 
CTGACCCGAG CAAGGCACCA GCACCACCTC CAGGGTTGCC TGGATGCCCT CGGCCACTAC 
AAGCAGTCAA AAGACCTGGC CCTGGCGGCA GAGGCGCTGC GGGTGGCCCG GGGTCACCTG 
ACCCGGCTCA CAGGTGGAGG GGGTACCGAG GAGATCCTGG ACATCATCTT CCAGGACTTC 
TGTGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( NM_001195422.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182351.1 Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant VII, mRNA.

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CloneID	OHu18549
Clone ID Related Accession (Same CDS sequence)	NM_001195422.1
Accession Version	NM_001195422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform VII
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC339966.1, AK297953.1, AK298485.1 and BC019261.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (VII) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant IV. The encoded isoform (VII) has a distinct N-terminus and is shorter than isoform IV. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297953.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153980, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGCATT CTCCAACTTG TCCCCACCCA TGCTTTCTTC TGGTCCCCGC CTCGGAGCCT 
CAGTTTCCCC ATCTGCAAAC TCCGGACCCT GGAGACGCTG TTTGGAATGT GAGATGGGCA 
TTGTGCACGC GCCGGAGCAG CGGCGCACCA GCCCCCGGCT CCGGCGCCAC CATCTTCGCG 
CTAAGCTCTG GCCAAGGCCG CTGCGGCATC GCAGTGATCC GGACCAGCGG CCCCGCCAGC 
GGCCACGCCC TCCGAATTCT CACAGCACCC CGAGACCTGC CCCTTGCTCG CCACGCCAGC 
CTGCGCCTGC TCAGCGATCC CCGCTCCGGG GAGCCTCTGG ACCGCGCACT GGTGCTCTGG 
TTCCCAGGTC CCCAGAGTTT CACCGGTGAG GACTGCGTGG AGTTCCACGT GCATGGAGGC 
CCGGCAGTGG TGAGCGGCGT CCTGCAGGCC TTGGGCAGCG TGCCAGGGCT TCGACCGGCG 
GAGGCAGGCG AGTTCACCAG ACGGGCGTTC GCCAATGGGA AGCTGAACCT GACCGAAGTG 
GAGGGGCTGG CGGACCTTAT CCACGCGGAA ACAGAGGCGC AGCGGCGGCA GGCCCTCAGG 
CAGCTGGACG GAGAGCTGGG CCACCTCTGC CGTGGCTGGG CCGAGACCCT CACCAAAGCT 
CTGGCCCACG TGGAGGCCTA TATCGATTTC GGCGAGGATG ACAACCTGGA GGAGGGGGTC 
CTGGAGCAAG CCGACATCGA AGTACGGGCA CTGCAGGTGG CCCTGGGTGC ACATCTACGA 
GATGCCAGGC GCGGGCAGAG GCTCCGCTCA GGGGTGCACG TAGTGGTCAC TGGACCCCCC 
AATGCGGGCA AGAGCAGCCT AGTGAACCTG CTCAGTCGGA AGCCTGTGTC CATCGTGTCC 
CCGGAGCCAG GGACCACCCG TGACGTGCTG GAGACCCCAG TCGACCTGGC CGGATTTCCT 
GTGCTGCTGA GCGACACGGC TGGGTTGCGG GAGGGCGTGG GGCCCGTGGA GCAGGAGGGC 
GTGCGGCGCG CCCGGGAGAG GCTAGAGCAG GCTGACCTCA TTCTGGCCAT GCTGGATGCT 
TCTGACCTGG CCTCTCCCTC CAGTTGCAAC TTCCTGGCCA CCGTCGTAGC CTCTGTGGGA 
GCCCAGAGCC CCAGTGACAG CAGCCAGCGC CTCCTCCTGG TGCTGAACAA GTCGGACCTG 
CTGTCCCCGG AGGGCCCAGG TCCCGGTCCT GACCTGCCCC CGCACCTGCT GCTGTCCTGT 
CTGACGGGAG AGGGGCTGGA CGGCCTCCTG GAGGCGCTGA GGAAGGAGCT AGCTGCAGTG 
TGTGGGGACC CGTCCACAGA TCCCCCGCTG CTGACCCGAG CAAGGCACCA GCACCACCTC 
CAGGGTTGCC TGGATGCCCT CGGCCACTAC AAGCAGTCAA AAGACCTGGC CCTGGCGGCA 
GAGGCGCTGC GGGTGGCCCG GGGTCACCTG ACCCGGCTCA CAGGTGGAGG GGGTACCGAG 
GAGATCCTGG ACATCATCTT CCAGGACTTC TGTGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182351.1
CDS	23..1567
Translation	MVHSPTCPHPCFLLVPASEPQFPHLQTPDPGDAVWNVRWALCTRRSSGAPAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALRILTAPRDLPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFANGKLNLTEVEGLADLIHAETEAQRRQALRQLDGELGHLCRGWAETLTKALAHVEAYIDFGEDDNLEEGVLEQADIEVRALQVALGAHLRDARRGQRLRSGVHVVVTGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPVLLSDTAGLREGVGPVEQEGVRRARERLEQADLILAMLDASDLASPSSCNFLATVVASVGAQSPSDSSQRLLLVLNKSDLLSPEGPGPGPDLPPHLLLSCLTGEGLDGLLEALRKELAAVCGDPSTDPPLLTRARHQHHLQGCLDALGHYKQSKDLALAAEALRVARGHLTRLTGGGGTEEILDIIFQDFCVGK

Target ORF information:

RefSeq Version	NM_001195422.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP binding protein 3, mitochondrial (GTPBP3), transcript variant VII, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195422.1

ORF Insert Sequence:

ATGGTGCATT CTCCAACTTG TCCCCACCCA TGCTTTCTTC TGGTCCCCGC CTCGGAGCCT 
CAGTTTCCCC ATCTGCAAAC TCCGGACCCT GGAGACGCTG TTTGGAATGT GAGATGGGCA 
TTGTGCACGC GCCGGAGCAG CGGCGCACCA GCCCCCGGCT CCGGCGCCAC CATCTTCGCG 
CTAAGCTCTG GCCAAGGCCG CTGCGGCATC GCAGTGATCC GGACCAGCGG CCCCGCCAGC 
GGCCACGCCC TCCGAATTCT CACAGCACCC CGAGACCTGC CCCTTGCTCG CCACGCCAGC 
CTGCGCCTGC TCAGCGATCC CCGCTCCGGG GAGCCTCTGG ACCGCGCACT GGTGCTCTGG 
TTCCCAGGTC CCCAGAGTTT CACCGGTGAG GACTGCGTGG AGTTCCACGT GCATGGAGGC 
CCGGCAGTGG TGAGCGGCGT CCTGCAGGCC TTGGGCAGCG TGCCAGGGCT TCGACCGGCG 
GAGGCAGGCG AGTTCACCAG ACGGGCGTTC GCCAATGGGA AGCTGAACCT GACCGAAGTG 
GAGGGGCTGG CGGACCTTAT CCACGCGGAA ACAGAGGCGC AGCGGCGGCA GGCCCTCAGG 
CAGCTGGACG GAGAGCTGGG CCACCTCTGC CGTGGCTGGG CCGAGACCCT CACCAAAGCT 
CTGGCCCACG TGGAGGCCTA TATCGATTTC GGCGAGGATG ACAACCTGGA GGAGGGGGTC 
CTGGAGCAAG CCGACATCGA AGTACGGGCA CTGCAGGTGG CCCTGGGTGC ACATCTACGA 
GATGCCAGGC GCGGGCAGAG GCTCCGCTCA GGGGTGCACG TAGTGGTCAC TGGACCCCCC 
AATGCGGGCA AGAGCAGCCT AGTGAACCTG CTCAGTCGGA AGCCTGTGTC CATCGTGTCC 
CCGGAGCCAG GGACCACCCG TGACGTGCTG GAGACCCCAG TCGACCTGGC CGGATTTCCT 
GTGCTGCTGA GCGACACGGC TGGGTTGCGG GAGGGCGTGG GGCCCGTGGA GCAGGAGGGC 
GTGCGGCGCG CCCGGGAGAG GCTAGAGCAG GCTGACCTCA TTCTGGCCAT GCTGGATGCT 
TCTGACCTGG CCTCTCCCTC CAGTTGCAAC TTCCTGGCCA CCGTCGTAGC CTCTGTGGGA 
GCCCAGAGCC CCAGTGACAG CAGCCAGCGC CTCCTCCTGG TGCTGAACAA GTCGGACCTG 
CTGTCCCCGG AGGGCCCAGG TCCCGGTCCT GACCTGCCCC CGCACCTGCT GCTGTCCTGT 
CTGACGGGAG AGGGGCTGGA CGGCCTCCTG GAGGCGCTGA GGAAGGAGCT AGCTGCAGTG 
TGTGGGGACC CGTCCACAGA TCCCCCGCTG CTGACCCGAG CAAGGCACCA GCACCACCTC 
CAGGGTTGCC TGGATGCCCT CGGCCACTAC AAGCAGTCAA AAGACCTGGC CCTGGCGGCA 
GAGGCGCTGC GGGTGGCCCG GGGTCACCTG ACCCGGCTCA CAGGTGGAGG GGGTACCGAG 
GAGATCCTGG ACATCATCTT CCAGGACTTC TGTGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPAR?-UCP2-AMPK axis.
Scientific reports8(1)1163(2018 Jan)
Boutoual R,Meseguer S,Villarroya M,Mart?n-Hern?ndez E,Errami M,Mart?n MA,Casado M,Armengod ME

microRNA-mediated differential expression of TRMU, GTPBP3 and MTO1 in cell models of mitochondrial-DNA diseases.
Scientific reports7(1)6209(2017 Jul)
Meseguer S,Boix O,Navarro-Gonz?lez C,Villarroya M,Boutoual R,Emperador S,Garc?a-Arum? E,Montoya J,Armengod ME

Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier.
PloS one10(12)e0144273(2015)
Mart?nez-Zamora A,Meseguer S,Esteve JM,Villarroya M,Aguado C,Enr?quez JA,Knecht E,Armengod ME

Mutations in GTPBP3 cause a mitochondrial translation defect associated with hypertrophic cardiomyopathy, lactic acidosis, and encephalopathy.
American journal of human genetics95(6)708-20(2014 Dec)
Kopajtich R,Nicholls TJ,Rorbach J,Metodiev MD,Freisinger P,Mandel H,Vanlander A,Ghezzi D,Carrozzo R,Taylor RW,Marquard K,Murayama K,Wieland T,Schwarzmayr T,Mayr JA,Pearce SF,Powell CA,Saada A,Ohtake A,Invernizzi F,Lamantea E,Sommerville EW,Pyle A,Chinnery PF,Crushell E,Okazaki Y,Kohda M,Kishita Y,Tokuzawa Y,Assouline Z,Rio M,Feillet F,Mousson de Camaret B,Chretien D,Munnich A,Menten B,Sante T,Smet J,R?gal L,Lorber A,Khoury A,Zeviani M,Strom TM,Meitinger T,Bertini ES,Van Coster R,Klopstock T,R?tig A,Haack TB,Minczuk M,Prokisch H

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GTPBP3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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