MCEE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCEE
Entrez Gene ID	84693
Full Name	methylmalonyl-CoA epimerase
Synonyms	GLOD2
General protein information	Preferred Names methylmalonyl-CoA epimerase Names methylmalonyl-CoA epimerase, mitochondrial DL-methylmalonyl-CoA racemase glyoxalase domain containing 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.3
Summary	The product of this gene catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids. odd chain-length fatty acids, and other metabolites. Mutations in this gene result in methylmalonyl-CoA epimerase deficiency, which is presented as mild to moderate methylmalonic aciduria. [provided by RefSeq, Jul 2008].
Disorder MIM:	608419
Disorder Html:	Methylmalonyl-CoA epimerase deficiency, 251120 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_032601.3	NP_115990.3	methylmalonyl-CoA epimerase, mitochondrial precursor
NM_032601.4	NP_115990.3	methylmalonyl-CoA epimerase, mitochondrial precursor
XM_005264613.2	XP_005264670.1	methylmalonyl-CoA epimerase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MCEE	NP_115990.3
Danio rerio (zebrafish)	mcee	NP_001018602.1
Pan troglodytes (chimpanzee)	MCEE	XP_515538.1
Mus musculus (house mouse)	Mcee	NP_082902.1
Gallus gallus (chicken)	MCEE	XP_413770.2
Xenopus tropicalis (tropical clawed frog)	mcee	NP_001004927.1
	MCEE	XP_001102303.1
Canis lupus familiaris (dog)	MCEE	XP_536170.4
Bos taurus (cattle)	MCEE	NP_001039460.1
Rattus norvegicus (Norway rat)	Mcee	NP_001099811.1
Caenorhabditis elegans (roundworm)	mce-1	NP_492120.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Atypical methylmalonic aciduria: frequency of mutations in the methylmalonyl CoA epimerase gene (MCEE).
Gradinger AB, B?lair C, Worgan LC, Li CD, Lavall?e J, Roquis D, Watkins D, Rosenblatt DS
Human mutation28(10)1045(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

analysis of frequency of mutations in the methylmalonyl CoA epimerase gene (MCEE) in atypical methylmalonic aciduria
Title: Atypical methylmalonic aciduria: frequency of mutations in the methylmalonyl CoA epimerase gene (MCEE).

The following MCEE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCEE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26303	NM_032601.4 Latest version!	Homo sapiens methylmalonyl-CoA epimerase (MCEE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu35637	XM_005264613.2 Latest version!	Homo sapiens methylmalonyl-CoA epimerase (MCEE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu26303	NM_032601.3	Homo sapiens methylmalonyl-CoA epimerase (MCEE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCEE ( NM_032601.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115990.3 Homo sapiens methylmalonyl-CoA epimerase (MCEE), mRNA.

Price & Availability↑

CloneID	OHu26303
Clone ID Related Accession (Same CDS sequence)	NM_032601.3 , NM_032601.4
Accession Version	NM_032601.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	methylmalonyl-CoA epimerase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM271757.1, AF364547.1, BC020825.1 and AI033764.1. This sequence is a reference standard in the RefSeqGene project. On May 14, 2008 this sequence version replaced NM_032601.2. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.120940.1, SRR1163655.692786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCGGG TGCTGAAGGC TGCAGCCGCG AATGCCGTAG GGCTTTTTTC CAGACTTCAA 
GCTCCCATTC CAACAGTAAG AGCTTCTTCC ACATCACAGC CCTTGGATCA AGTGACAGGT 
TCTGTGTGGA ACCTGGGTCG ACTCAACCAT GTAGCCATAG CAGTGCCAGA TTTGGAAAAG 
GCTGCAGCAT TTTATAAGAA TATTCTGGGG GCCCAGGTAA GTGAAGCGGT CCCTCTTCCT 
GAACATGGAG TATCTGTTGT TTTTGTCAAC CTGGGAAATA CCAAGATGGA ACTGCTTCAT 
CCATTGGGAC GTGACAGTCC AATTGCAGGT TTTCTGCAGA AAAACAAGGC TGGAGGAATG 
CATCACATCT GCATCGAGGT GGATAATATT AATGCAGCTG TGATGGATTT GAAAAAAAAG 
AAGATCCGCA GTCTAAGTGA AGAGGTCAAA ATAGGAGCAC ATGGAAAACC AGTGATTTTT 
CTCCATCCTA AAGACTGTGG TGGAGTCCTT GTGGAACTGG AGCAAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115990.3
CDS	46..576
Translation	MARVLKAAAANAVGLFSRLQAPIPTVRASSTSQPLDQVTGSVWNLGRLNHVAIAVPDLEKAAAFYKNILGAQVSEAVPLPEHGVSVVFVNLGNTKMELLHPLGRDSPIAGFLQKNKAGGMHHICIEVDNINAAVMDLKKKKIRSLSEEVKIGAHGKPVIFLHPKDCGGVLVELEQA

Target ORF information:

RefSeq Version	NM_032601.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methylmalonyl-CoA epimerase (MCEE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032601.3

ORF Insert Sequence:

ATGGCGCGGG TGCTGAAGGC TGCAGCCGCG AATGCCGTAG GGCTTTTTTC CAGACTTCAA 
GCTCCCATTC CAACAGTAAG AGCTTCTTCC ACATCACAGC CCTTGGATCA AGTGACAGGT 
TCTGTGTGGA ACCTGGGTCG ACTCAACCAT GTAGCCATAG CAGTGCCAGA TTTGGAAAAG 
GCTGCAGCAT TTTATAAGAA TATTCTGGGG GCCCAGGTAA GTGAAGCGGT CCCTCTTCCT 
GAACATGGAG TATCTGTTGT TTTTGTCAAC CTGGGAAATA CCAAGATGGA ACTGCTTCAT 
CCATTGGGAC GTGACAGTCC AATTGCAGGT TTTCTGCAGA AAAACAAGGC TGGAGGAATG 
CATCACATCT GCATCGAGGT GGATAATATT AATGCAGCTG TGATGGATTT GAAAAAAAAG 
AAGATCCGCA GTCTAAGTGA AGAGGTCAAA ATAGGAGCAC ATGGAAAACC AGTGATTTTT 
CTCCATCCTA AAGACTGTGG TGGAGTCCTT GTGGAACTGG AGCAAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCEE ( NM_032601.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115990.3 Homo sapiens methylmalonyl-CoA epimerase (MCEE), mRNA.

Price & Availability↑

CloneID	OHu26303
Clone ID Related Accession (Same CDS sequence)	NM_032601.3 , NM_032601.4
Accession Version	NM_032601.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	methylmalonyl-CoA epimerase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF364547.1, BC020825.1 and AI033764.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_032601.3. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.63614.1, SRR5189661.77625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000244217.6/ ENSP00000244217.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCGGG TGCTGAAGGC TGCAGCCGCG AATGCCGTAG GGCTTTTTTC CAGACTTCAA 
GCTCCCATTC CAACAGTAAG AGCTTCTTCC ACATCACAGC CCTTGGATCA AGTGACAGGT 
TCTGTGTGGA ACCTGGGTCG ACTCAACCAT GTAGCCATAG CAGTGCCAGA TTTGGAAAAG 
GCTGCAGCAT TTTATAAGAA TATTCTGGGG GCCCAGGTAA GTGAAGCGGT CCCTCTTCCT 
GAACATGGAG TATCTGTTGT TTTTGTCAAC CTGGGAAATA CCAAGATGGA ACTGCTTCAT 
CCATTGGGAC GTGACAGTCC AATTGCAGGT TTTCTGCAGA AAAACAAGGC TGGAGGAATG 
CATCACATCT GCATCGAGGT GGATAATATT AATGCAGCTG TGATGGATTT GAAAAAAAAG 
AAGATCCGCA GTCTAAGTGA AGAGGTCAAA ATAGGAGCAC ATGGAAAACC AGTGATTTTT 
CTCCATCCTA AAGACTGTGG TGGAGTCCTT GTGGAACTGG AGCAAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115990.3
CDS	11..541
Translation	MARVLKAAAANAVGLFSRLQAPIPTVRASSTSQPLDQVTGSVWNLGRLNHVAIAVPDLEKAAAFYKNILGAQVSEAVPLPEHGVSVVFVNLGNTKMELLHPLGRDSPIAGFLQKNKAGGMHHICIEVDNINAAVMDLKKKKIRSLSEEVKIGAHGKPVIFLHPKDCGGVLVELEQA

Target ORF information:

RefSeq Version	NM_032601.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methylmalonyl-CoA epimerase (MCEE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032601.4

ORF Insert Sequence:

ATGGCGCGGG TGCTGAAGGC TGCAGCCGCG AATGCCGTAG GGCTTTTTTC CAGACTTCAA 
GCTCCCATTC CAACAGTAAG AGCTTCTTCC ACATCACAGC CCTTGGATCA AGTGACAGGT 
TCTGTGTGGA ACCTGGGTCG ACTCAACCAT GTAGCCATAG CAGTGCCAGA TTTGGAAAAG 
GCTGCAGCAT TTTATAAGAA TATTCTGGGG GCCCAGGTAA GTGAAGCGGT CCCTCTTCCT 
GAACATGGAG TATCTGTTGT TTTTGTCAAC CTGGGAAATA CCAAGATGGA ACTGCTTCAT 
CCATTGGGAC GTGACAGTCC AATTGCAGGT TTTCTGCAGA AAAACAAGGC TGGAGGAATG 
CATCACATCT GCATCGAGGT GGATAATATT AATGCAGCTG TGATGGATTT GAAAAAAAAG 
AAGATCCGCA GTCTAAGTGA AGAGGTCAAA ATAGGAGCAC ATGGAAAACC AGTGATTTTT 
CTCCATCCTA AAGACTGTGG TGGAGTCCTT GTGGAACTGG AGCAAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCEE ( XM_005264613.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264670.1 Homo sapiens methylmalonyl-CoA epimerase (MCEE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu35637
Clone ID Related Accession (Same CDS sequence)	XM_005264613.2
Accession Version	XM_005264613.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methylmalonyl-CoA epimerase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG TGCTGAAGGC TGCAGCCGCG AATGCCGTAG GGCTTTTTTC CAGACTTCAA 
GCTCCCATTC CAACAGTAAG AGCTTCTTCC ACATCACAGC CCTTGGATCA AGTGACAGGT 
TCTGTGTGGA ACCTGGGTCG ACTCAACCAT GTAGCCATAG CAGTGCCAGA TTTGGAAAAG 
GCTGCAGCAT TTTATAAGAA TATTCTGGGG GCCCAGGTGG ATAATATTAA TGCAGCTGTG 
ATGGATTTGA AAAAAAAGAA GATCCGCAGT CTAAGTGAAG AGGTCAAAAT AGGAGCACAT 
GGAAAACCAG TGATTTTTCT CCATCCTAAA GACTGTGGTG GAGTCCTTGT GGAACTGGAG 
CAAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264670.1
CDS	70..438
Translation	MARVLKAAAANAVGLFSRLQAPIPTVRASSTSQPLDQVTGSVWNLGRLNHVAIAVPDLEKAAAFYKNILGAQVDNINAAVMDLKKKKIRSLSEEVKIGAHGKPVIFLHPKDCGGVLVELEQA

Target ORF information:

RefSeq Version	XM_005264613.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methylmalonyl-CoA epimerase (MCEE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264613.2

ORF Insert Sequence:

ATGGCGCGGG TGCTGAAGGC TGCAGCCGCG AATGCCGTAG GGCTTTTTTC CAGACTTCAA 
GCTCCCATTC CAACAGTAAG AGCTTCTTCC ACATCACAGC CCTTGGATCA AGTGACAGGT 
TCTGTGTGGA ACCTGGGTCG ACTCAACCAT GTAGCCATAG CAGTGCCAGA TTTGGAAAAG 
GCTGCAGCAT TTTATAAGAA TATTCTGGGG GCCCAGGTGG ATAATATTAA TGCAGCTGTG 
ATGGATTTGA AAAAAAAGAA GATCCGCAGT CTAAGTGAAG AGGTCAAAAT AGGAGCACAT 
GGAAAACCAG TGATTTTTCT CCATCCTAAA GACTGTGGTG GAGTCCTTGT GGAACTGGAG 
CAAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Atypical methylmalonic aciduria: frequency of mutations in the methylmalonyl CoA epimerase gene (MCEE).
Human mutation28(10)1045(2007 Oct)
Gradinger AB,B?lair C,Worgan LC,Li CD,Lavall?e J,Roquis D,Watkins D,Rosenblatt DS

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MCEE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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