GNPAT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNPAT
Entrez Gene ID	8443
Full Name	glyceronephosphate O-acyltransferase
Synonyms	DAP-AT,DAPAT,DHAPAT,RCDP2
General protein information	Preferred Names glyceronephosphate O-acyltransferase Names dihydroxyacetone phosphate acyltransferase DHAP-AT acyl-CoA:dihydroxyacetonephosphateacyltransferase glycerone-phosphate O-acyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.2
Summary	This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015].
Disorder MIM:	602744
Disorder Html:	Rhizomelic chondrodysplasia punctata, type 2, 222765 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011544303.2	XP_011542605.1	dihydroxyacetone phosphate acyltransferase isoform X2
NM_001316350.1	NP_001303279.1	dihydroxyacetone phosphate acyltransferase isoform 2
NM_014236.3	NP_055051.1	dihydroxyacetone phosphate acyltransferase isoform 1
XM_005273313.4	XP_005273370.1	dihydroxyacetone phosphate acyltransferase isoform X1
XM_011544303.3	XP_011542605.1	dihydroxyacetone phosphate acyltransferase isoform X2
NM_001316350.2	NP_001303279.1	dihydroxyacetone phosphate acyltransferase isoform 2
XM_011544304.2	XP_011542606.1	dihydroxyacetone phosphate acyltransferase isoform X2
NM_014236.4	NP_055051.1	dihydroxyacetone phosphate acyltransferase isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GNPAT	XP_536346.3
Drosophila melanogaster (fruit fly)	Dhap-at	NP_001262957.1
Bos taurus (cattle)	GNPAT	NP_001096756.1
Rattus norvegicus (Norway rat)	Gnpat	NP_445862.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001908	XP_321157.5
Magnaporthe oryzae (rice blast fungus)	MGG_01894	XP_003714915.1
Xenopus tropicalis (tropical clawed frog)	gnpat	NP_001073039.1
Homo sapiens (human)	GNPAT	NP_055051.1
Pan troglodytes (chimpanzee)	GNPAT	XP_001151001.1
Neurospora crassa	NCU11282	XP_001728326.1
Xenopus tropicalis (tropical clawed frog)	LOC100494829	XP_002941187.1
	GNPAT	XP_001106100.2
Mus musculus (house mouse)	Gnpat	NP_034452.3
Gallus gallus (chicken)	GNPAT	XP_419589.4
Danio rerio (zebrafish)	gnpat	XP_002665694.2
Caenorhabditis elegans (roundworm)	acl-7	NP_496725.2

Gene Ontology
Bibliography

Related articles in PubMed

GNPAT rs11558492 is not a Major Modifier of Iron Status: Study of Italian Hemochromatosis Patients and Blood Donors.
Greni F, Valenti L, Mariani R, Pelloni I, Rametta R, Busti F, Ravasi G, Girelli D, Fargion S, Galimberti S, Piperno A, Pelucchi S
Annals of hepatology16(3)451-456(2017 May - Jun)

Reduction of Ether-Type Glycerophospholipids, Plasmalogens, by NF-?B Signal Leading to Microglial Activation.
Hossain MS, Abe Y, Ali F, Youssef M, Honsho M, Fujiki Y, Katafuchi T
The Journal of neuroscience : the official journal of the Society for Neuroscience37(15)4074-4092(2017 Apr)

GNPAT p.D519G is independently associated with markedly increased iron stores in HFE p.C282Y homozygotes.
Barton JC, Chen WP, Emond MJ, Phatak PD, Subramaniam VN, Adams PC, Gurrin LC, Anderson GJ, Ramm GA, Powell LW, Allen KJ, Phillips JD, Parker CJ, McLaren GD, McLaren CE
Blood cells, molecules & diseases6315-20(2017 Mar)

Recombinant human dihydroxyacetonephosphate acyl-transferase characterization as an integral monotopic membrane protein.
Piano V, Nenci S, Magnani F, Aliverti A, Mattevi A
Biochemical and biophysical research communications481(1-2)51-58(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GNPAT rs11558492 is not a major modifier of iron status and is not associated with liver fibrosis in HFE-related Hemochromatosis patients.
Title: GNPAT rs11558492 is not a Major Modifier of Iron Status: Study of Italian Hemochromatosis Patients and Blood Donors.

C282Y homozygotes referred for HFE testing commonly have a GNPAT variant. This GNPAT variant does not appear be a co-modifying gene affecting expression of HFE related hemochromatosis in this population. The GNPAT variant does not predict the severity of iron overload.
Title: ?GNPAT variant (D519G) is not associated with an elevated serum ferritin or iron removed by phlebotomy in patients referred for C282Y-linked hemochromatosis.

Reduction of GNPAT activated NF-kappaB in glial cell lines and microglia in cortex.
Title: Reduction of Ether-Type Glycerophospholipids, Plasmalogens, by NF-?B Signal Leading to Microglial Activation.

GNPAT p.D519G is strongly associated with markedly increased iron stores in p.C282Y homozygotes after correction for age, iron-related variables, and alcohol consumption.
Title: GNPAT p.D519G is independently associated with markedly increased iron stores in HFE p.C282Y homozygotes.

Here, we report the characterization of the recombinant human DHAP acyl-transferase, which performs the first step in alkyl-DHAP synthesis.
Title: Recombinant human dihydroxyacetonephosphate acyl-transferase characterization as an integral monotopic membrane protein.

The following GNPAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNPAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27084	NM_014236.4 Latest version!	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu35136	NM_001316350.2 Latest version!	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu61773	XM_011544303.3 Latest version!	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu61773	XM_011544304.2 Latest version!	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu35135	XM_005273313.4 Latest version!	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu61773	XM_011544303.2	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu35136	NM_001316350.1	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu27084	NM_014236.3	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNPAT ( NM_014236.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055051.1 Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27084
Clone ID Related Accession (Same CDS sequence)	NM_014236.3 , NM_014236.4
Accession Version	NM_014236.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	dihydroxyacetone phosphate acyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137801.11, AJ002190.1 and BE222957.1. This sequence is a reference standard in the RefSeqGene project. On Mar 21, 2008 this sequence version replaced NM_014236.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.18115.1, SRR1803611.156149.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGAA GGAGCTCAAA AAGTGGGATG AGTTTGAAGA TATTTTAGAA 
GAGAGGAGGC ATGTCAGTGA CTTGAAATTT GCAATGAAAT GCTACACACC TCTTGTCTAT 
AAGGGAATTA CTCCATGTAA ACCAATTGAT ATTAAATGTA GTGTTCTCAA TTCTGAGGAG 
ATTCATTATG TCATTAAACA GCTTTCCAAG GAATCCCTTC AATCTGTGGA TGTCCTCCGA 
GAGGAAGTGA GTGAGATCTT AGATGAAATG AGTCACAAAC TGCGTCTTGG AGCCATTCGG 
TTTTGTGCCT TCACCCTGAG CAAAGTATTT AAACAAATTT TCTCGAAGGT GTGTGTAAAT 
GAAGAAGGTA TTCAGAAACT ACAAAGAGCC ATCCAGGAGC ATCCTGTTGT TCTGCTGCCT 
AGTCATCGAA GTTACATTGA CTTCCTCATG TTGTCTTTTC TTCTATACAA TTATGATTTG 
CCTGTGCCAG TTATAGCAGC AGGAATGGAC TTCCTGGGAA TGAAAATGGT TGGTGAGCTG 
CTACGAATGT CGGGTGCCTT TTTCATGCGG CGTACCTTTG GTGGCAATAA ACTCTACTGG 
GCTGTATTCT CTGAATATGT AAAAACTATG TTACGGAATG GTTATGCTCC TGTTGAATTT 
TTCCTCGAAG GGACAAGAAG CCGCTCTGCC AAGACATTGA CTCCTAAATT TGGTCTTCTG 
AATATTGTGA TGGAGCCATT TTTTAAAAGA GAAGTTTTTG ATACCTACCT TGTCCCAATT 
AGTATCAGTT ATGATAAGAT CTTGGAAGAA ACTCTTTATG TGTATGAGCT TCTAGGGGTT 
CCTAAACCAA AAGAGTCTAC AACTGGGTTG CTGAAAGCCA GAAAGATTCT CTCTGAAAAT 
TTTGGAAGCA TCCATGTGTA CTTTGGAGAT CCTGTGTCAC TTCGATCTTT GGCAGCTGGG 
AGGATGAGTC GGAGCTCATA TAACTTGGTT CCAAGATACA TTCCTCAGAA ACAGTCTGAG 
GACATGCATG CCTTTGTCAC TGAAGTTGCC TACAAAATGG AGCTTCTGCA AATTGAAAAC 
ATGGTTTTGA GCCCCTGGAC CCTAATAGTT GCTGTTCTGC TTCAGAACCG GCCATCCATG 
GACTTTGATG CTCTGGTGGA AAAGACTTTA TGGCTAAAAG GCTTAACCCA GGCATTTGGA 
GGGTTTCTCA TTTGGCCTGA TAATAAACCT GCTGAAGAAG TTGTCCCGGC CAGCATTCTT 
CTGCATTCCA ACATTGCCAG CCTTGTCAAA GACCAGGTGA TTCTGAAAGT GGACTCCGGA 
GACTCGGAAG TGGTCGATGG GCTTATGCTC CAGCACATCA CTCTCCTCAT GTGCTCAGCT 
TATAGGAACC AGCTGCTCAA CATTTTTGTG CGCCCATCCT TAGTAGCAGT AGCATTGCAG 
ATGACACCAG GGTTCAGGAA AGAGGATGTC TACAGTTGCT TTCGCTTCCT ACGTGATGTT 
TTTGCAGATG AGTTCATCTT CCTTCCAGGA AACACACTAA AGGACTTTGA AGAAGGCTGT 
TACCTGCTTT GTAAAAGTGA AGCCATACAA GTGACTACGA AAGACATCCT AGTTACAGAG 
AAAGGAAATA CTGTGTTAGA ATTTTTAGTA GGACTCTTTA AACCTTTTGT GGAAAGCTAT 
CAGATAATTT GCAAGTACCT TTTGAGTGAA GAAGAGGACC ACTTCAGTGA GGAACAGTAC 
TTGGCTGCAG TCAGAAAATT CACAAGTCAG CTTCTCGATC AAGGTACCTC TCAATGTTAT 
GATGTATTAT CTTCTGATGT GCAGAAAAAC GCCTTAGCAG CCTGTGTGAG GCTCGGAGTA 
GTGGAGAAGA AGAAGATAAA TAATAACTGT ATATTTAATG TGAATGAACC TGCCACAACC 
AAATTAGAAG AAATGCTTGG TTGTAAGACA CCAATAGGAA AACCAGCCAC TGCAAAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055051.1
CDS	207..2249
Translation	MESSSSSNSYFSVGPTSPSAVVLLYSKELKKWDEFEDILEERRHVSDLKFAMKCYTPLVYKGITPCKPIDIKCSVLNSEEIHYVIKQLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

Target ORF information:

RefSeq Version	NM_014236.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014236.3

ORF Insert Sequence:

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGAA GGAGCTCAAA AAGTGGGATG AGTTTGAAGA TATTTTAGAA 
GAGAGGAGGC ATGTCAGTGA CTTGAAATTT GCAATGAAAT GCTACACACC TCTTGTCTAT 
AAGGGAATTA CTCCATGTAA ACCAATTGAT ATTAAATGTA GTGTTCTCAA TTCTGAGGAG 
ATTCATTATG TCATTAAACA GCTTTCCAAG GAATCCCTTC AATCTGTGGA TGTCCTCCGA 
GAGGAAGTGA GTGAGATCTT AGATGAAATG AGTCACAAAC TGCGTCTTGG AGCCATTCGG 
TTTTGTGCCT TCACCCTGAG CAAAGTATTT AAACAAATTT TCTCGAAGGT GTGTGTAAAT 
GAAGAAGGTA TTCAGAAACT ACAAAGAGCC ATCCAGGAGC ATCCTGTTGT TCTGCTGCCT 
AGTCATCGAA GTTACATTGA CTTCCTCATG TTGTCTTTTC TTCTATACAA TTATGATTTG 
CCTGTGCCAG TTATAGCAGC AGGAATGGAC TTCCTGGGAA TGAAAATGGT TGGTGAGCTG 
CTACGAATGT CGGGTGCCTT TTTCATGCGG CGTACCTTTG GTGGCAATAA ACTCTACTGG 
GCTGTATTCT CTGAATATGT AAAAACTATG TTACGGAATG GTTATGCTCC TGTTGAATTT 
TTCCTCGAAG GGACAAGAAG CCGCTCTGCC AAGACATTGA CTCCTAAATT TGGTCTTCTG 
AATATTGTGA TGGAGCCATT TTTTAAAAGA GAAGTTTTTG ATACCTACCT TGTCCCAATT 
AGTATCAGTT ATGATAAGAT CTTGGAAGAA ACTCTTTATG TGTATGAGCT TCTAGGGGTT 
CCTAAACCAA AAGAGTCTAC AACTGGGTTG CTGAAAGCCA GAAAGATTCT CTCTGAAAAT 
TTTGGAAGCA TCCATGTGTA CTTTGGAGAT CCTGTGTCAC TTCGATCTTT GGCAGCTGGG 
AGGATGAGTC GGAGCTCATA TAACTTGGTT CCAAGATACA TTCCTCAGAA ACAGTCTGAG 
GACATGCATG CCTTTGTCAC TGAAGTTGCC TACAAAATGG AGCTTCTGCA AATTGAAAAC 
ATGGTTTTGA GCCCCTGGAC CCTAATAGTT GCTGTTCTGC TTCAGAACCG GCCATCCATG 
GACTTTGATG CTCTGGTGGA AAAGACTTTA TGGCTAAAAG GCTTAACCCA GGCATTTGGA 
GGGTTTCTCA TTTGGCCTGA TAATAAACCT GCTGAAGAAG TTGTCCCGGC CAGCATTCTT 
CTGCATTCCA ACATTGCCAG CCTTGTCAAA GACCAGGTGA TTCTGAAAGT GGACTCCGGA 
GACTCGGAAG TGGTCGATGG GCTTATGCTC CAGCACATCA CTCTCCTCAT GTGCTCAGCT 
TATAGGAACC AGCTGCTCAA CATTTTTGTG CGCCCATCCT TAGTAGCAGT AGCATTGCAG 
ATGACACCAG GGTTCAGGAA AGAGGATGTC TACAGTTGCT TTCGCTTCCT ACGTGATGTT 
TTTGCAGATG AGTTCATCTT CCTTCCAGGA AACACACTAA AGGACTTTGA AGAAGGCTGT 
TACCTGCTTT GTAAAAGTGA AGCCATACAA GTGACTACGA AAGACATCCT AGTTACAGAG 
AAAGGAAATA CTGTGTTAGA ATTTTTAGTA GGACTCTTTA AACCTTTTGT GGAAAGCTAT 
CAGATAATTT GCAAGTACCT TTTGAGTGAA GAAGAGGACC ACTTCAGTGA GGAACAGTAC 
TTGGCTGCAG TCAGAAAATT CACAAGTCAG CTTCTCGATC AAGGTACCTC TCAATGTTAT 
GATGTATTAT CTTCTGATGT GCAGAAAAAC GCCTTAGCAG CCTGTGTGAG GCTCGGAGTA 
GTGGAGAAGA AGAAGATAAA TAATAACTGT ATATTTAATG TGAATGAACC TGCCACAACC 
AAATTAGAAG AAATGCTTGG TTGTAAGACA CCAATAGGAA AACCAGCCAC TGCAAAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNPAT ( NM_014236.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055051.1 Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27084
Clone ID Related Accession (Same CDS sequence)	NM_014236.3 , NM_014236.4
Accession Version	NM_014236.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	dihydroxyacetone phosphate acyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137801.11, AJ002190.1 and BE222957.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_014236.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.18115.1, SRR1660803.105724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366647.9/ ENSP00000355607.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGAA GGAGCTCAAA AAGTGGGATG AGTTTGAAGA TATTTTAGAA 
GAGAGGAGGC ATGTCAGTGA CTTGAAATTT GCAATGAAAT GCTACACACC TCTTGTCTAT 
AAGGGAATTA CTCCATGTAA ACCAATTGAT ATTAAATGTA GTGTTCTCAA TTCTGAGGAG 
ATTCATTATG TCATTAAACA GCTTTCCAAG GAATCCCTTC AATCTGTGGA TGTCCTCCGA 
GAGGAAGTGA GTGAGATCTT AGATGAAATG AGTCACAAAC TGCGTCTTGG AGCCATTCGG 
TTTTGTGCCT TCACCCTGAG CAAAGTATTT AAACAAATTT TCTCGAAGGT GTGTGTAAAT 
GAAGAAGGTA TTCAGAAACT ACAAAGAGCC ATCCAGGAGC ATCCTGTTGT TCTGCTGCCT 
AGTCATCGAA GTTACATTGA CTTCCTCATG TTGTCTTTTC TTCTATACAA TTATGATTTG 
CCTGTGCCAG TTATAGCAGC AGGAATGGAC TTCCTGGGAA TGAAAATGGT TGGTGAGCTG 
CTACGAATGT CGGGTGCCTT TTTCATGCGG CGTACCTTTG GTGGCAATAA ACTCTACTGG 
GCTGTATTCT CTGAATATGT AAAAACTATG TTACGGAATG GTTATGCTCC TGTTGAATTT 
TTCCTCGAAG GGACAAGAAG CCGCTCTGCC AAGACATTGA CTCCTAAATT TGGTCTTCTG 
AATATTGTGA TGGAGCCATT TTTTAAAAGA GAAGTTTTTG ATACCTACCT TGTCCCAATT 
AGTATCAGTT ATGATAAGAT CTTGGAAGAA ACTCTTTATG TGTATGAGCT TCTAGGGGTT 
CCTAAACCAA AAGAGTCTAC AACTGGGTTG CTGAAAGCCA GAAAGATTCT CTCTGAAAAT 
TTTGGAAGCA TCCATGTGTA CTTTGGAGAT CCTGTGTCAC TTCGATCTTT GGCAGCTGGG 
AGGATGAGTC GGAGCTCATA TAACTTGGTT CCAAGATACA TTCCTCAGAA ACAGTCTGAG 
GACATGCATG CCTTTGTCAC TGAAGTTGCC TACAAAATGG AGCTTCTGCA AATTGAAAAC 
ATGGTTTTGA GCCCCTGGAC CCTAATAGTT GCTGTTCTGC TTCAGAACCG GCCATCCATG 
GACTTTGATG CTCTGGTGGA AAAGACTTTA TGGCTAAAAG GCTTAACCCA GGCATTTGGA 
GGGTTTCTCA TTTGGCCTGA TAATAAACCT GCTGAAGAAG TTGTCCCGGC CAGCATTCTT 
CTGCATTCCA ACATTGCCAG CCTTGTCAAA GACCAGGTGA TTCTGAAAGT GGACTCCGGA 
GACTCGGAAG TGGTCGATGG GCTTATGCTC CAGCACATCA CTCTCCTCAT GTGCTCAGCT 
TATAGGAACC AGCTGCTCAA CATTTTTGTG CGCCCATCCT TAGTAGCAGT AGCATTGCAG 
ATGACACCAG GGTTCAGGAA AGAGGATGTC TACAGTTGCT TTCGCTTCCT ACGTGATGTT 
TTTGCAGATG AGTTCATCTT CCTTCCAGGA AACACACTAA AGGACTTTGA AGAAGGCTGT 
TACCTGCTTT GTAAAAGTGA AGCCATACAA GTGACTACGA AAGACATCCT AGTTACAGAG 
AAAGGAAATA CTGTGTTAGA ATTTTTAGTA GGACTCTTTA AACCTTTTGT GGAAAGCTAT 
CAGATAATTT GCAAGTACCT TTTGAGTGAA GAAGAGGACC ACTTCAGTGA GGAACAGTAC 
TTGGCTGCAG TCAGAAAATT CACAAGTCAG CTTCTCGATC AAGGTACCTC TCAATGTTAT 
GATGTATTAT CTTCTGATGT GCAGAAAAAC GCCTTAGCAG CCTGTGTGAG GCTCGGAGTA 
GTGGAGAAGA AGAAGATAAA TAATAACTGT ATATTTAATG TGAATGAACC TGCCACAACC 
AAATTAGAAG AAATGCTTGG TTGTAAGACA CCAATAGGAA AACCAGCCAC TGCAAAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055051.1
CDS	168..2210
Translation	MESSSSSNSYFSVGPTSPSAVVLLYSKELKKWDEFEDILEERRHVSDLKFAMKCYTPLVYKGITPCKPIDIKCSVLNSEEIHYVIKQLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

Target ORF information:

RefSeq Version	NM_014236.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014236.4

ORF Insert Sequence:

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGAA GGAGCTCAAA AAGTGGGATG AGTTTGAAGA TATTTTAGAA 
GAGAGGAGGC ATGTCAGTGA CTTGAAATTT GCAATGAAAT GCTACACACC TCTTGTCTAT 
AAGGGAATTA CTCCATGTAA ACCAATTGAT ATTAAATGTA GTGTTCTCAA TTCTGAGGAG 
ATTCATTATG TCATTAAACA GCTTTCCAAG GAATCCCTTC AATCTGTGGA TGTCCTCCGA 
GAGGAAGTGA GTGAGATCTT AGATGAAATG AGTCACAAAC TGCGTCTTGG AGCCATTCGG 
TTTTGTGCCT TCACCCTGAG CAAAGTATTT AAACAAATTT TCTCGAAGGT GTGTGTAAAT 
GAAGAAGGTA TTCAGAAACT ACAAAGAGCC ATCCAGGAGC ATCCTGTTGT TCTGCTGCCT 
AGTCATCGAA GTTACATTGA CTTCCTCATG TTGTCTTTTC TTCTATACAA TTATGATTTG 
CCTGTGCCAG TTATAGCAGC AGGAATGGAC TTCCTGGGAA TGAAAATGGT TGGTGAGCTG 
CTACGAATGT CGGGTGCCTT TTTCATGCGG CGTACCTTTG GTGGCAATAA ACTCTACTGG 
GCTGTATTCT CTGAATATGT AAAAACTATG TTACGGAATG GTTATGCTCC TGTTGAATTT 
TTCCTCGAAG GGACAAGAAG CCGCTCTGCC AAGACATTGA CTCCTAAATT TGGTCTTCTG 
AATATTGTGA TGGAGCCATT TTTTAAAAGA GAAGTTTTTG ATACCTACCT TGTCCCAATT 
AGTATCAGTT ATGATAAGAT CTTGGAAGAA ACTCTTTATG TGTATGAGCT TCTAGGGGTT 
CCTAAACCAA AAGAGTCTAC AACTGGGTTG CTGAAAGCCA GAAAGATTCT CTCTGAAAAT 
TTTGGAAGCA TCCATGTGTA CTTTGGAGAT CCTGTGTCAC TTCGATCTTT GGCAGCTGGG 
AGGATGAGTC GGAGCTCATA TAACTTGGTT CCAAGATACA TTCCTCAGAA ACAGTCTGAG 
GACATGCATG CCTTTGTCAC TGAAGTTGCC TACAAAATGG AGCTTCTGCA AATTGAAAAC 
ATGGTTTTGA GCCCCTGGAC CCTAATAGTT GCTGTTCTGC TTCAGAACCG GCCATCCATG 
GACTTTGATG CTCTGGTGGA AAAGACTTTA TGGCTAAAAG GCTTAACCCA GGCATTTGGA 
GGGTTTCTCA TTTGGCCTGA TAATAAACCT GCTGAAGAAG TTGTCCCGGC CAGCATTCTT 
CTGCATTCCA ACATTGCCAG CCTTGTCAAA GACCAGGTGA TTCTGAAAGT GGACTCCGGA 
GACTCGGAAG TGGTCGATGG GCTTATGCTC CAGCACATCA CTCTCCTCAT GTGCTCAGCT 
TATAGGAACC AGCTGCTCAA CATTTTTGTG CGCCCATCCT TAGTAGCAGT AGCATTGCAG 
ATGACACCAG GGTTCAGGAA AGAGGATGTC TACAGTTGCT TTCGCTTCCT ACGTGATGTT 
TTTGCAGATG AGTTCATCTT CCTTCCAGGA AACACACTAA AGGACTTTGA AGAAGGCTGT 
TACCTGCTTT GTAAAAGTGA AGCCATACAA GTGACTACGA AAGACATCCT AGTTACAGAG 
AAAGGAAATA CTGTGTTAGA ATTTTTAGTA GGACTCTTTA AACCTTTTGT GGAAAGCTAT 
CAGATAATTT GCAAGTACCT TTTGAGTGAA GAAGAGGACC ACTTCAGTGA GGAACAGTAC 
TTGGCTGCAG TCAGAAAATT CACAAGTCAG CTTCTCGATC AAGGTACCTC TCAATGTTAT 
GATGTATTAT CTTCTGATGT GCAGAAAAAC GCCTTAGCAG CCTGTGTGAG GCTCGGAGTA 
GTGGAGAAGA AGAAGATAAA TAATAACTGT ATATTTAATG TGAATGAACC TGCCACAACC 
AAATTAGAAG AAATGCTTGG TTGTAAGACA CCAATAGGAA AACCAGCCAC TGCAAAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNPAT ( NM_001316350.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303279.1 Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 2, mRNA.

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CloneID	OHu35136
Clone ID Related Accession (Same CDS sequence)	NM_001316350.1 , NM_001316350.2
Accession Version	NM_001316350.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	dihydroxyacetone phosphate acyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA552987.1, DC366380.1, AK297982.1, AL137801.11 and BE222957.1. On Oct 16, 2015 this sequence version replaced XM_005273314.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.114486.1, SRR1803616.278739.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGCT TTCCAAGGAA TCCCTTCAAT CTGTGGATGT CCTCCGAGAG 
GAAGTGAGTG AGATCTTAGA TGAAATGAGT CACAAACTGC GTCTTGGAGC CATTCGGTTT 
TGTGCCTTCA CCCTGAGCAA AGTATTTAAA CAAATTTTCT CGAAGGTGTG TGTAAATGAA 
GAAGGTATTC AGAAACTACA AAGAGCCATC CAGGAGCATC CTGTTGTTCT GCTGCCTAGT 
CATCGAAGTT ACATTGACTT CCTCATGTTG TCTTTTCTTC TATACAATTA TGATTTGCCT 
GTGCCAGTTA TAGCAGCAGG AATGGACTTC CTGGGAATGA AAATGGTTGG TGAGCTGCTA 
CGAATGTCGG GTGCCTTTTT CATGCGGCGT ACCTTTGGTG GCAATAAACT CTACTGGGCT 
GTATTCTCTG AATATGTAAA AACTATGTTA CGGAATGGTT ATGCTCCTGT TGAATTTTTC 
CTCGAAGGGA CAAGAAGCCG CTCTGCCAAG ACATTGACTC CTAAATTTGG TCTTCTGAAT 
ATTGTGATGG AGCCATTTTT TAAAAGAGAA GTTTTTGATA CCTACCTTGT CCCAATTAGT 
ATCAGTTATG ATAAGATCTT GGAAGAAACT CTTTATGTGT ATGAGCTTCT AGGGGTTCCT 
AAACCAAAAG AGTCTACAAC TGGGTTGCTG AAAGCCAGAA AGATTCTCTC TGAAAATTTT 
GGAAGCATCC ATGTGTACTT TGGAGATCCT GTGTCACTTC GATCTTTGGC AGCTGGGAGG 
ATGAGTCGGA GCTCATATAA CTTGGTTCCA AGATACATTC CTCAGAAACA GTCTGAGGAC 
ATGCATGCCT TTGTCACTGA AGTTGCCTAC AAAATGGAGC TTCTGCAAAT TGAAAACATG 
GTTTTGAGCC CCTGGACCCT AATAGTTGCT GTTCTGCTTC AGAACCGGCC ATCCATGGAC 
TTTGATGCTC TGGTGGAAAA GACTTTATGG CTAAAAGGCT TAACCCAGGC ATTTGGAGGG 
TTTCTCATTT GGCCTGATAA TAAACCTGCT GAAGAAGTTG TCCCGGCCAG CATTCTTCTG 
CATTCCAACA TTGCCAGCCT TGTCAAAGAC CAGGTGATTC TGAAAGTGGA CTCCGGAGAC 
TCGGAAGTGG TCGATGGGCT TATGCTCCAG CACATCACTC TCCTCATGTG CTCAGCTTAT 
AGGAACCAGC TGCTCAACAT TTTTGTGCGC CCATCCTTAG TAGCAGTAGC ATTGCAGATG 
ACACCAGGGT TCAGGAAAGA GGATGTCTAC AGTTGCTTTC GCTTCCTACG TGATGTTTTT 
GCAGATGAGT TCATCTTCCT TCCAGGAAAC ACACTAAAGG ACTTTGAAGA AGGCTGTTAC 
CTGCTTTGTA AAAGTGAAGC CATACAAGTG ACTACGAAAG ACATCCTAGT TACAGAGAAA 
GGAAATACTG TGTTAGAATT TTTAGTAGGA CTCTTTAAAC CTTTTGTGGA AAGCTATCAG 
ATAATTTGCA AGTACCTTTT GAGTGAAGAA GAGGACCACT TCAGTGAGGA ACAGTACTTG 
GCTGCAGTCA GAAAATTCAC AAGTCAGCTT CTCGATCAAG GTACCTCTCA ATGTTATGAT 
GTATTATCTT CTGATGTGCA GAAAAACGCC TTAGCAGCCT GTGTGAGGCT CGGAGTAGTG 
GAGAAGAAGA AGATAAATAA TAACTGTATA TTTAATGTGA ATGAACCTGC CACAACCAAA 
TTAGAAGAAA TGCTTGGTTG TAAGACACCA ATAGGAAAAC CAGCCACTGC AAAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303279.1
CDS	207..2066
Translation	MESSSSSNSYFSVGPTSPSAVVLLYSLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

Target ORF information:

RefSeq Version	NM_001316350.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316350.1

ORF Insert Sequence:

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGCT TTCCAAGGAA TCCCTTCAAT CTGTGGATGT CCTCCGAGAG 
GAAGTGAGTG AGATCTTAGA TGAAATGAGT CACAAACTGC GTCTTGGAGC CATTCGGTTT 
TGTGCCTTCA CCCTGAGCAA AGTATTTAAA CAAATTTTCT CGAAGGTGTG TGTAAATGAA 
GAAGGTATTC AGAAACTACA AAGAGCCATC CAGGAGCATC CTGTTGTTCT GCTGCCTAGT 
CATCGAAGTT ACATTGACTT CCTCATGTTG TCTTTTCTTC TATACAATTA TGATTTGCCT 
GTGCCAGTTA TAGCAGCAGG AATGGACTTC CTGGGAATGA AAATGGTTGG TGAGCTGCTA 
CGAATGTCGG GTGCCTTTTT CATGCGGCGT ACCTTTGGTG GCAATAAACT CTACTGGGCT 
GTATTCTCTG AATATGTAAA AACTATGTTA CGGAATGGTT ATGCTCCTGT TGAATTTTTC 
CTCGAAGGGA CAAGAAGCCG CTCTGCCAAG ACATTGACTC CTAAATTTGG TCTTCTGAAT 
ATTGTGATGG AGCCATTTTT TAAAAGAGAA GTTTTTGATA CCTACCTTGT CCCAATTAGT 
ATCAGTTATG ATAAGATCTT GGAAGAAACT CTTTATGTGT ATGAGCTTCT AGGGGTTCCT 
AAACCAAAAG AGTCTACAAC TGGGTTGCTG AAAGCCAGAA AGATTCTCTC TGAAAATTTT 
GGAAGCATCC ATGTGTACTT TGGAGATCCT GTGTCACTTC GATCTTTGGC AGCTGGGAGG 
ATGAGTCGGA GCTCATATAA CTTGGTTCCA AGATACATTC CTCAGAAACA GTCTGAGGAC 
ATGCATGCCT TTGTCACTGA AGTTGCCTAC AAAATGGAGC TTCTGCAAAT TGAAAACATG 
GTTTTGAGCC CCTGGACCCT AATAGTTGCT GTTCTGCTTC AGAACCGGCC ATCCATGGAC 
TTTGATGCTC TGGTGGAAAA GACTTTATGG CTAAAAGGCT TAACCCAGGC ATTTGGAGGG 
TTTCTCATTT GGCCTGATAA TAAACCTGCT GAAGAAGTTG TCCCGGCCAG CATTCTTCTG 
CATTCCAACA TTGCCAGCCT TGTCAAAGAC CAGGTGATTC TGAAAGTGGA CTCCGGAGAC 
TCGGAAGTGG TCGATGGGCT TATGCTCCAG CACATCACTC TCCTCATGTG CTCAGCTTAT 
AGGAACCAGC TGCTCAACAT TTTTGTGCGC CCATCCTTAG TAGCAGTAGC ATTGCAGATG 
ACACCAGGGT TCAGGAAAGA GGATGTCTAC AGTTGCTTTC GCTTCCTACG TGATGTTTTT 
GCAGATGAGT TCATCTTCCT TCCAGGAAAC ACACTAAAGG ACTTTGAAGA AGGCTGTTAC 
CTGCTTTGTA AAAGTGAAGC CATACAAGTG ACTACGAAAG ACATCCTAGT TACAGAGAAA 
GGAAATACTG TGTTAGAATT TTTAGTAGGA CTCTTTAAAC CTTTTGTGGA AAGCTATCAG 
ATAATTTGCA AGTACCTTTT GAGTGAAGAA GAGGACCACT TCAGTGAGGA ACAGTACTTG 
GCTGCAGTCA GAAAATTCAC AAGTCAGCTT CTCGATCAAG GTACCTCTCA ATGTTATGAT 
GTATTATCTT CTGATGTGCA GAAAAACGCC TTAGCAGCCT GTGTGAGGCT CGGAGTAGTG 
GAGAAGAAGA AGATAAATAA TAACTGTATA TTTAATGTGA ATGAACCTGC CACAACCAAA 
TTAGAAGAAA TGCTTGGTTG TAAGACACCA ATAGGAAAAC CAGCCACTGC AAAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNPAT ( NM_001316350.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303279.1 Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 2, mRNA.

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CloneID	OHu35136
Clone ID Related Accession (Same CDS sequence)	NM_001316350.1 , NM_001316350.2
Accession Version	NM_001316350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	dihydroxyacetone phosphate acyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC366380.1, AK297982.1, AL137801.11 and BE222957.1. On May 31, 2019 this sequence version replaced NM_001316350.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297982.1, SRR1660805.114486.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGCT TTCCAAGGAA TCCCTTCAAT CTGTGGATGT CCTCCGAGAG 
GAAGTGAGTG AGATCTTAGA TGAAATGAGT CACAAACTGC GTCTTGGAGC CATTCGGTTT 
TGTGCCTTCA CCCTGAGCAA AGTATTTAAA CAAATTTTCT CGAAGGTGTG TGTAAATGAA 
GAAGGTATTC AGAAACTACA AAGAGCCATC CAGGAGCATC CTGTTGTTCT GCTGCCTAGT 
CATCGAAGTT ACATTGACTT CCTCATGTTG TCTTTTCTTC TATACAATTA TGATTTGCCT 
GTGCCAGTTA TAGCAGCAGG AATGGACTTC CTGGGAATGA AAATGGTTGG TGAGCTGCTA 
CGAATGTCGG GTGCCTTTTT CATGCGGCGT ACCTTTGGTG GCAATAAACT CTACTGGGCT 
GTATTCTCTG AATATGTAAA AACTATGTTA CGGAATGGTT ATGCTCCTGT TGAATTTTTC 
CTCGAAGGGA CAAGAAGCCG CTCTGCCAAG ACATTGACTC CTAAATTTGG TCTTCTGAAT 
ATTGTGATGG AGCCATTTTT TAAAAGAGAA GTTTTTGATA CCTACCTTGT CCCAATTAGT 
ATCAGTTATG ATAAGATCTT GGAAGAAACT CTTTATGTGT ATGAGCTTCT AGGGGTTCCT 
AAACCAAAAG AGTCTACAAC TGGGTTGCTG AAAGCCAGAA AGATTCTCTC TGAAAATTTT 
GGAAGCATCC ATGTGTACTT TGGAGATCCT GTGTCACTTC GATCTTTGGC AGCTGGGAGG 
ATGAGTCGGA GCTCATATAA CTTGGTTCCA AGATACATTC CTCAGAAACA GTCTGAGGAC 
ATGCATGCCT TTGTCACTGA AGTTGCCTAC AAAATGGAGC TTCTGCAAAT TGAAAACATG 
GTTTTGAGCC CCTGGACCCT AATAGTTGCT GTTCTGCTTC AGAACCGGCC ATCCATGGAC 
TTTGATGCTC TGGTGGAAAA GACTTTATGG CTAAAAGGCT TAACCCAGGC ATTTGGAGGG 
TTTCTCATTT GGCCTGATAA TAAACCTGCT GAAGAAGTTG TCCCGGCCAG CATTCTTCTG 
CATTCCAACA TTGCCAGCCT TGTCAAAGAC CAGGTGATTC TGAAAGTGGA CTCCGGAGAC 
TCGGAAGTGG TCGATGGGCT TATGCTCCAG CACATCACTC TCCTCATGTG CTCAGCTTAT 
AGGAACCAGC TGCTCAACAT TTTTGTGCGC CCATCCTTAG TAGCAGTAGC ATTGCAGATG 
ACACCAGGGT TCAGGAAAGA GGATGTCTAC AGTTGCTTTC GCTTCCTACG TGATGTTTTT 
GCAGATGAGT TCATCTTCCT TCCAGGAAAC ACACTAAAGG ACTTTGAAGA AGGCTGTTAC 
CTGCTTTGTA AAAGTGAAGC CATACAAGTG ACTACGAAAG ACATCCTAGT TACAGAGAAA 
GGAAATACTG TGTTAGAATT TTTAGTAGGA CTCTTTAAAC CTTTTGTGGA AAGCTATCAG 
ATAATTTGCA AGTACCTTTT GAGTGAAGAA GAGGACCACT TCAGTGAGGA ACAGTACTTG 
GCTGCAGTCA GAAAATTCAC AAGTCAGCTT CTCGATCAAG GTACCTCTCA ATGTTATGAT 
GTATTATCTT CTGATGTGCA GAAAAACGCC TTAGCAGCCT GTGTGAGGCT CGGAGTAGTG 
GAGAAGAAGA AGATAAATAA TAACTGTATA TTTAATGTGA ATGAACCTGC CACAACCAAA 
TTAGAAGAAA TGCTTGGTTG TAAGACACCA ATAGGAAAAC CAGCCACTGC AAAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303279.1
CDS	168..2027
Translation	MESSSSSNSYFSVGPTSPSAVVLLYSLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

Target ORF information:

RefSeq Version	NM_001316350.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316350.2

ORF Insert Sequence:

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGCT TTCCAAGGAA TCCCTTCAAT CTGTGGATGT CCTCCGAGAG 
GAAGTGAGTG AGATCTTAGA TGAAATGAGT CACAAACTGC GTCTTGGAGC CATTCGGTTT 
TGTGCCTTCA CCCTGAGCAA AGTATTTAAA CAAATTTTCT CGAAGGTGTG TGTAAATGAA 
GAAGGTATTC AGAAACTACA AAGAGCCATC CAGGAGCATC CTGTTGTTCT GCTGCCTAGT 
CATCGAAGTT ACATTGACTT CCTCATGTTG TCTTTTCTTC TATACAATTA TGATTTGCCT 
GTGCCAGTTA TAGCAGCAGG AATGGACTTC CTGGGAATGA AAATGGTTGG TGAGCTGCTA 
CGAATGTCGG GTGCCTTTTT CATGCGGCGT ACCTTTGGTG GCAATAAACT CTACTGGGCT 
GTATTCTCTG AATATGTAAA AACTATGTTA CGGAATGGTT ATGCTCCTGT TGAATTTTTC 
CTCGAAGGGA CAAGAAGCCG CTCTGCCAAG ACATTGACTC CTAAATTTGG TCTTCTGAAT 
ATTGTGATGG AGCCATTTTT TAAAAGAGAA GTTTTTGATA CCTACCTTGT CCCAATTAGT 
ATCAGTTATG ATAAGATCTT GGAAGAAACT CTTTATGTGT ATGAGCTTCT AGGGGTTCCT 
AAACCAAAAG AGTCTACAAC TGGGTTGCTG AAAGCCAGAA AGATTCTCTC TGAAAATTTT 
GGAAGCATCC ATGTGTACTT TGGAGATCCT GTGTCACTTC GATCTTTGGC AGCTGGGAGG 
ATGAGTCGGA GCTCATATAA CTTGGTTCCA AGATACATTC CTCAGAAACA GTCTGAGGAC 
ATGCATGCCT TTGTCACTGA AGTTGCCTAC AAAATGGAGC TTCTGCAAAT TGAAAACATG 
GTTTTGAGCC CCTGGACCCT AATAGTTGCT GTTCTGCTTC AGAACCGGCC ATCCATGGAC 
TTTGATGCTC TGGTGGAAAA GACTTTATGG CTAAAAGGCT TAACCCAGGC ATTTGGAGGG 
TTTCTCATTT GGCCTGATAA TAAACCTGCT GAAGAAGTTG TCCCGGCCAG CATTCTTCTG 
CATTCCAACA TTGCCAGCCT TGTCAAAGAC CAGGTGATTC TGAAAGTGGA CTCCGGAGAC 
TCGGAAGTGG TCGATGGGCT TATGCTCCAG CACATCACTC TCCTCATGTG CTCAGCTTAT 
AGGAACCAGC TGCTCAACAT TTTTGTGCGC CCATCCTTAG TAGCAGTAGC ATTGCAGATG 
ACACCAGGGT TCAGGAAAGA GGATGTCTAC AGTTGCTTTC GCTTCCTACG TGATGTTTTT 
GCAGATGAGT TCATCTTCCT TCCAGGAAAC ACACTAAAGG ACTTTGAAGA AGGCTGTTAC 
CTGCTTTGTA AAAGTGAAGC CATACAAGTG ACTACGAAAG ACATCCTAGT TACAGAGAAA 
GGAAATACTG TGTTAGAATT TTTAGTAGGA CTCTTTAAAC CTTTTGTGGA AAGCTATCAG 
ATAATTTGCA AGTACCTTTT GAGTGAAGAA GAGGACCACT TCAGTGAGGA ACAGTACTTG 
GCTGCAGTCA GAAAATTCAC AAGTCAGCTT CTCGATCAAG GTACCTCTCA ATGTTATGAT 
GTATTATCTT CTGATGTGCA GAAAAACGCC TTAGCAGCCT GTGTGAGGCT CGGAGTAGTG 
GAGAAGAAGA AGATAAATAA TAACTGTATA TTTAATGTGA ATGAACCTGC CACAACCAAA 
TTAGAAGAAA TGCTTGGTTG TAAGACACCA ATAGGAAAAC CAGCCACTGC AAAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNPAT ( XM_011544303.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542605.1 Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X2, mRNA.

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CloneID	OHu61773
Clone ID Related Accession (Same CDS sequence)	XM_011544303.2 , XM_011544303.3 , XM_011544304.2
Accession Version	XM_011544303.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	dihydroxyacetone phosphate acyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544303.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCACA AACTGCGTCT TGGAGCCATT CGGTTTTGTG CCTTCACCCT GAGCAAAGTA 
TTTAAACAAA TTTTCTCGAA GGTGTGTGTA AATGAAGAAG GTATTCAGAA ACTACAAAGA 
GCCATCCAGG AGCATCCTGT TGTTCTGCTG CCTAGTCATC GAAGTTACAT TGACTTCCTC 
ATGTTGTCTT TTCTTCTATA CAATTATGAT TTGCCTGTGC CAGTTATAGC AGCAGGAATG 
GACTTCCTGG GAATGAAAAT GGTTGGTGAG CTGCTACGAA TGTCGGGTGC CTTTTTCATG 
CGGCGTACCT TTGGTGGCAA TAAACTCTAC TGGGCTGTAT TCTCTGAATA TGTAAAAACT 
ATGTTACGGA ATGGTTATGC TCCTGTTGAA TTTTTCCTCG AAGGGACAAG AAGCCGCTCT 
GCCAAGACAT TGACTCCTAA ATTTGGTCTT CTGAATATTG TGATGGAGCC ATTTTTTAAA 
AGAGAAGTTT TTGATACCTA CCTTGTCCCA ATTAGTATCA GTTATGATAA GATCTTGGAA 
GAAACTCTTT ATGTGTATGA GCTTCTAGGG GTTCCTAAAC CAAAAGAGTC TACAACTGGG 
TTGCTGAAAG CCAGAAAGAT TCTCTCTGAA AATTTTGGAA GCATCCATGT GTACTTTGGA 
GATCCTGTGT CACTTCGATC TTTGGCAGCT GGGAGGATGA GTCGGAGCTC ATATAACTTG 
GTTCCAAGAT ACATTCCTCA GAAACAGTCT GAGGACATGC ATGCCTTTGT CACTGAAGTT 
GCCTACAAAA TGGAGCTTCT GCAAATTGAA AACATGGTTT TGAGCCCCTG GACCCTAATA 
GTTGCTGTTC TGCTTCAGAA CCGGCCATCC ATGGACTTTG ATGCTCTGGT GGAAAAGACT 
TTATGGCTAA AAGGCTTAAC CCAGGCATTT GGAGGGTTTC TCATTTGGCC TGATAATAAA 
CCTGCTGAAG AAGTTGTCCC GGCCAGCATT CTTCTGCATT CCAACATTGC CAGCCTTGTC 
AAAGACCAGG TGATTCTGAA AGTGGACTCC GGAGACTCGG AAGTGGTCGA TGGGCTTATG 
CTCCAGCACA TCACTCTCCT CATGTGCTCA GCTTATAGGA ACCAGCTGCT CAACATTTTT 
GTGCGCCCAT CCTTAGTAGC AGTAGCATTG CAGATGACAC CAGGGTTCAG GAAAGAGGAT 
GTCTACAGTT GCTTTCGCTT CCTACGTGAT GTTTTTGCAG ATGAGTTCAT CTTCCTTCCA 
GGAAACACAC TAAAGGACTT TGAAGAAGGC TGTTACCTGC TTTGTAAAAG TGAAGCCATA 
CAAGTGACTA CGAAAGACAT CCTAGTTACA GAGAAAGGAA ATACTGTGTT AGAATTTTTA 
GTAGGACTCT TTAAACCTTT TGTGGAAAGC TATCAGATAA TTTGCAAGTA CCTTTTGAGT 
GAAGAAGAGG ACCACTTCAG TGAGGAACAG TACTTGGCTG CAGTCAGAAA ATTCACAAGT 
CAGCTTCTCG ATCAAGGTAC CTCTCAATGT TATGATGTAT TATCTTCTGA TGTGCAGAAA 
AACGCCTTAG CAGCCTGTGT GAGGCTCGGA GTAGTGGAGA AGAAGAAGAT AAATAATAAC 
TGTATATTTA ATGTGAATGA ACCTGCCACA ACCAAATTAG AAGAAATGCT TGGTTGTAAG 
ACACCAATAG GAAAACCAGC CACTGCAAAA CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542605.1
CDS	583..2298
Translation	MSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

Target ORF information:

RefSeq Version	XM_011544303.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544303.2

ORF Insert Sequence:

ATGAGTCACA AACTGCGTCT TGGAGCCATT CGGTTTTGTG CCTTCACCCT GAGCAAAGTA 
TTTAAACAAA TTTTCTCGAA GGTGTGTGTA AATGAAGAAG GTATTCAGAA ACTACAAAGA 
GCCATCCAGG AGCATCCTGT TGTTCTGCTG CCTAGTCATC GAAGTTACAT TGACTTCCTC 
ATGTTGTCTT TTCTTCTATA CAATTATGAT TTGCCTGTGC CAGTTATAGC AGCAGGAATG 
GACTTCCTGG GAATGAAAAT GGTTGGTGAG CTGCTACGAA TGTCGGGTGC CTTTTTCATG 
CGGCGTACCT TTGGTGGCAA TAAACTCTAC TGGGCTGTAT TCTCTGAATA TGTAAAAACT 
ATGTTACGGA ATGGTTATGC TCCTGTTGAA TTTTTCCTCG AAGGGACAAG AAGCCGCTCT 
GCCAAGACAT TGACTCCTAA ATTTGGTCTT CTGAATATTG TGATGGAGCC ATTTTTTAAA 
AGAGAAGTTT TTGATACCTA CCTTGTCCCA ATTAGTATCA GTTATGATAA GATCTTGGAA 
GAAACTCTTT ATGTGTATGA GCTTCTAGGG GTTCCTAAAC CAAAAGAGTC TACAACTGGG 
TTGCTGAAAG CCAGAAAGAT TCTCTCTGAA AATTTTGGAA GCATCCATGT GTACTTTGGA 
GATCCTGTGT CACTTCGATC TTTGGCAGCT GGGAGGATGA GTCGGAGCTC ATATAACTTG 
GTTCCAAGAT ACATTCCTCA GAAACAGTCT GAGGACATGC ATGCCTTTGT CACTGAAGTT 
GCCTACAAAA TGGAGCTTCT GCAAATTGAA AACATGGTTT TGAGCCCCTG GACCCTAATA 
GTTGCTGTTC TGCTTCAGAA CCGGCCATCC ATGGACTTTG ATGCTCTGGT GGAAAAGACT 
TTATGGCTAA AAGGCTTAAC CCAGGCATTT GGAGGGTTTC TCATTTGGCC TGATAATAAA 
CCTGCTGAAG AAGTTGTCCC GGCCAGCATT CTTCTGCATT CCAACATTGC CAGCCTTGTC 
AAAGACCAGG TGATTCTGAA AGTGGACTCC GGAGACTCGG AAGTGGTCGA TGGGCTTATG 
CTCCAGCACA TCACTCTCCT CATGTGCTCA GCTTATAGGA ACCAGCTGCT CAACATTTTT 
GTGCGCCCAT CCTTAGTAGC AGTAGCATTG CAGATGACAC CAGGGTTCAG GAAAGAGGAT 
GTCTACAGTT GCTTTCGCTT CCTACGTGAT GTTTTTGCAG ATGAGTTCAT CTTCCTTCCA 
GGAAACACAC TAAAGGACTT TGAAGAAGGC TGTTACCTGC TTTGTAAAAG TGAAGCCATA 
CAAGTGACTA CGAAAGACAT CCTAGTTACA GAGAAAGGAA ATACTGTGTT AGAATTTTTA 
GTAGGACTCT TTAAACCTTT TGTGGAAAGC TATCAGATAA TTTGCAAGTA CCTTTTGAGT 
GAAGAAGAGG ACCACTTCAG TGAGGAACAG TACTTGGCTG CAGTCAGAAA ATTCACAAGT 
CAGCTTCTCG ATCAAGGTAC CTCTCAATGT TATGATGTAT TATCTTCTGA TGTGCAGAAA 
AACGCCTTAG CAGCCTGTGT GAGGCTCGGA GTAGTGGAGA AGAAGAAGAT AAATAATAAC 
TGTATATTTA ATGTGAATGA ACCTGCCACA ACCAAATTAG AAGAAATGCT TGGTTGTAAG 
ACACCAATAG GAAAACCAGC CACTGCAAAA CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNPAT ( XM_011544303.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542605.1 Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu61773
Clone ID Related Accession (Same CDS sequence)	XM_011544303.2 , XM_011544303.3 , XM_011544304.2
Accession Version	XM_011544303.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dihydroxyacetone phosphate acyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544303.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCACA AACTGCGTCT TGGAGCCATT CGGTTTTGTG CCTTCACCCT GAGCAAAGTA 
TTTAAACAAA TTTTCTCGAA GGTGTGTGTA AATGAAGAAG GTATTCAGAA ACTACAAAGA 
GCCATCCAGG AGCATCCTGT TGTTCTGCTG CCTAGTCATC GAAGTTACAT TGACTTCCTC 
ATGTTGTCTT TTCTTCTATA CAATTATGAT TTGCCTGTGC CAGTTATAGC AGCAGGAATG 
GACTTCCTGG GAATGAAAAT GGTTGGTGAG CTGCTACGAA TGTCGGGTGC CTTTTTCATG 
CGGCGTACCT TTGGTGGCAA TAAACTCTAC TGGGCTGTAT TCTCTGAATA TGTAAAAACT 
ATGTTACGGA ATGGTTATGC TCCTGTTGAA TTTTTCCTCG AAGGGACAAG AAGCCGCTCT 
GCCAAGACAT TGACTCCTAA ATTTGGTCTT CTGAATATTG TGATGGAGCC ATTTTTTAAA 
AGAGAAGTTT TTGATACCTA CCTTGTCCCA ATTAGTATCA GTTATGATAA GATCTTGGAA 
GAAACTCTTT ATGTGTATGA GCTTCTAGGG GTTCCTAAAC CAAAAGAGTC TACAACTGGG 
TTGCTGAAAG CCAGAAAGAT TCTCTCTGAA AATTTTGGAA GCATCCATGT GTACTTTGGA 
GATCCTGTGT CACTTCGATC TTTGGCAGCT GGGAGGATGA GTCGGAGCTC ATATAACTTG 
GTTCCAAGAT ACATTCCTCA GAAACAGTCT GAGGACATGC ATGCCTTTGT CACTGAAGTT 
GCCTACAAAA TGGAGCTTCT GCAAATTGAA AACATGGTTT TGAGCCCCTG GACCCTAATA 
GTTGCTGTTC TGCTTCAGAA CCGGCCATCC ATGGACTTTG ATGCTCTGGT GGAAAAGACT 
TTATGGCTAA AAGGCTTAAC CCAGGCATTT GGAGGGTTTC TCATTTGGCC TGATAATAAA 
CCTGCTGAAG AAGTTGTCCC GGCCAGCATT CTTCTGCATT CCAACATTGC CAGCCTTGTC 
AAAGACCAGG TGATTCTGAA AGTGGACTCC GGAGACTCGG AAGTGGTCGA TGGGCTTATG 
CTCCAGCACA TCACTCTCCT CATGTGCTCA GCTTATAGGA ACCAGCTGCT CAACATTTTT 
GTGCGCCCAT CCTTAGTAGC AGTAGCATTG CAGATGACAC CAGGGTTCAG GAAAGAGGAT 
GTCTACAGTT GCTTTCGCTT CCTACGTGAT GTTTTTGCAG ATGAGTTCAT CTTCCTTCCA 
GGAAACACAC TAAAGGACTT TGAAGAAGGC TGTTACCTGC TTTGTAAAAG TGAAGCCATA 
CAAGTGACTA CGAAAGACAT CCTAGTTACA GAGAAAGGAA ATACTGTGTT AGAATTTTTA 
GTAGGACTCT TTAAACCTTT TGTGGAAAGC TATCAGATAA TTTGCAAGTA CCTTTTGAGT 
GAAGAAGAGG ACCACTTCAG TGAGGAACAG TACTTGGCTG CAGTCAGAAA ATTCACAAGT 
CAGCTTCTCG ATCAAGGTAC CTCTCAATGT TATGATGTAT TATCTTCTGA TGTGCAGAAA 
AACGCCTTAG CAGCCTGTGT GAGGCTCGGA GTAGTGGAGA AGAAGAAGAT AAATAATAAC 
TGTATATTTA ATGTGAATGA ACCTGCCACA ACCAAATTAG AAGAAATGCT TGGTTGTAAG 
ACACCAATAG GAAAACCAGC CACTGCAAAA CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542605.1
CDS	579..2294
Translation	MSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

Target ORF information:

RefSeq Version	XM_011544303.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544303.3

ORF Insert Sequence:

ATGAGTCACA AACTGCGTCT TGGAGCCATT CGGTTTTGTG CCTTCACCCT GAGCAAAGTA 
TTTAAACAAA TTTTCTCGAA GGTGTGTGTA AATGAAGAAG GTATTCAGAA ACTACAAAGA 
GCCATCCAGG AGCATCCTGT TGTTCTGCTG CCTAGTCATC GAAGTTACAT TGACTTCCTC 
ATGTTGTCTT TTCTTCTATA CAATTATGAT TTGCCTGTGC CAGTTATAGC AGCAGGAATG 
GACTTCCTGG GAATGAAAAT GGTTGGTGAG CTGCTACGAA TGTCGGGTGC CTTTTTCATG 
CGGCGTACCT TTGGTGGCAA TAAACTCTAC TGGGCTGTAT TCTCTGAATA TGTAAAAACT 
ATGTTACGGA ATGGTTATGC TCCTGTTGAA TTTTTCCTCG AAGGGACAAG AAGCCGCTCT 
GCCAAGACAT TGACTCCTAA ATTTGGTCTT CTGAATATTG TGATGGAGCC ATTTTTTAAA 
AGAGAAGTTT TTGATACCTA CCTTGTCCCA ATTAGTATCA GTTATGATAA GATCTTGGAA 
GAAACTCTTT ATGTGTATGA GCTTCTAGGG GTTCCTAAAC CAAAAGAGTC TACAACTGGG 
TTGCTGAAAG CCAGAAAGAT TCTCTCTGAA AATTTTGGAA GCATCCATGT GTACTTTGGA 
GATCCTGTGT CACTTCGATC TTTGGCAGCT GGGAGGATGA GTCGGAGCTC ATATAACTTG 
GTTCCAAGAT ACATTCCTCA GAAACAGTCT GAGGACATGC ATGCCTTTGT CACTGAAGTT 
GCCTACAAAA TGGAGCTTCT GCAAATTGAA AACATGGTTT TGAGCCCCTG GACCCTAATA 
GTTGCTGTTC TGCTTCAGAA CCGGCCATCC ATGGACTTTG ATGCTCTGGT GGAAAAGACT 
TTATGGCTAA AAGGCTTAAC CCAGGCATTT GGAGGGTTTC TCATTTGGCC TGATAATAAA 
CCTGCTGAAG AAGTTGTCCC GGCCAGCATT CTTCTGCATT CCAACATTGC CAGCCTTGTC 
AAAGACCAGG TGATTCTGAA AGTGGACTCC GGAGACTCGG AAGTGGTCGA TGGGCTTATG 
CTCCAGCACA TCACTCTCCT CATGTGCTCA GCTTATAGGA ACCAGCTGCT CAACATTTTT 
GTGCGCCCAT CCTTAGTAGC AGTAGCATTG CAGATGACAC CAGGGTTCAG GAAAGAGGAT 
GTCTACAGTT GCTTTCGCTT CCTACGTGAT GTTTTTGCAG ATGAGTTCAT CTTCCTTCCA 
GGAAACACAC TAAAGGACTT TGAAGAAGGC TGTTACCTGC TTTGTAAAAG TGAAGCCATA 
CAAGTGACTA CGAAAGACAT CCTAGTTACA GAGAAAGGAA ATACTGTGTT AGAATTTTTA 
GTAGGACTCT TTAAACCTTT TGTGGAAAGC TATCAGATAA TTTGCAAGTA CCTTTTGAGT 
GAAGAAGAGG ACCACTTCAG TGAGGAACAG TACTTGGCTG CAGTCAGAAA ATTCACAAGT 
CAGCTTCTCG ATCAAGGTAC CTCTCAATGT TATGATGTAT TATCTTCTGA TGTGCAGAAA 
AACGCCTTAG CAGCCTGTGT GAGGCTCGGA GTAGTGGAGA AGAAGAAGAT AAATAATAAC 
TGTATATTTA ATGTGAATGA ACCTGCCACA ACCAAATTAG AAGAAATGCT TGGTTGTAAG 
ACACCAATAG GAAAACCAGC CACTGCAAAA CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNPAT ( XM_011544304.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542606.1 Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu61773
Clone ID Related Accession (Same CDS sequence)	XM_011544303.2 , XM_011544303.3 , XM_011544304.2
Accession Version	XM_011544304.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dihydroxyacetone phosphate acyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544304.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCACA AACTGCGTCT TGGAGCCATT CGGTTTTGTG CCTTCACCCT GAGCAAAGTA 
TTTAAACAAA TTTTCTCGAA GGTGTGTGTA AATGAAGAAG GTATTCAGAA ACTACAAAGA 
GCCATCCAGG AGCATCCTGT TGTTCTGCTG CCTAGTCATC GAAGTTACAT TGACTTCCTC 
ATGTTGTCTT TTCTTCTATA CAATTATGAT TTGCCTGTGC CAGTTATAGC AGCAGGAATG 
GACTTCCTGG GAATGAAAAT GGTTGGTGAG CTGCTACGAA TGTCGGGTGC CTTTTTCATG 
CGGCGTACCT TTGGTGGCAA TAAACTCTAC TGGGCTGTAT TCTCTGAATA TGTAAAAACT 
ATGTTACGGA ATGGTTATGC TCCTGTTGAA TTTTTCCTCG AAGGGACAAG AAGCCGCTCT 
GCCAAGACAT TGACTCCTAA ATTTGGTCTT CTGAATATTG TGATGGAGCC ATTTTTTAAA 
AGAGAAGTTT TTGATACCTA CCTTGTCCCA ATTAGTATCA GTTATGATAA GATCTTGGAA 
GAAACTCTTT ATGTGTATGA GCTTCTAGGG GTTCCTAAAC CAAAAGAGTC TACAACTGGG 
TTGCTGAAAG CCAGAAAGAT TCTCTCTGAA AATTTTGGAA GCATCCATGT GTACTTTGGA 
GATCCTGTGT CACTTCGATC TTTGGCAGCT GGGAGGATGA GTCGGAGCTC ATATAACTTG 
GTTCCAAGAT ACATTCCTCA GAAACAGTCT GAGGACATGC ATGCCTTTGT CACTGAAGTT 
GCCTACAAAA TGGAGCTTCT GCAAATTGAA AACATGGTTT TGAGCCCCTG GACCCTAATA 
GTTGCTGTTC TGCTTCAGAA CCGGCCATCC ATGGACTTTG ATGCTCTGGT GGAAAAGACT 
TTATGGCTAA AAGGCTTAAC CCAGGCATTT GGAGGGTTTC TCATTTGGCC TGATAATAAA 
CCTGCTGAAG AAGTTGTCCC GGCCAGCATT CTTCTGCATT CCAACATTGC CAGCCTTGTC 
AAAGACCAGG TGATTCTGAA AGTGGACTCC GGAGACTCGG AAGTGGTCGA TGGGCTTATG 
CTCCAGCACA TCACTCTCCT CATGTGCTCA GCTTATAGGA ACCAGCTGCT CAACATTTTT 
GTGCGCCCAT CCTTAGTAGC AGTAGCATTG CAGATGACAC CAGGGTTCAG GAAAGAGGAT 
GTCTACAGTT GCTTTCGCTT CCTACGTGAT GTTTTTGCAG ATGAGTTCAT CTTCCTTCCA 
GGAAACACAC TAAAGGACTT TGAAGAAGGC TGTTACCTGC TTTGTAAAAG TGAAGCCATA 
CAAGTGACTA CGAAAGACAT CCTAGTTACA GAGAAAGGAA ATACTGTGTT AGAATTTTTA 
GTAGGACTCT TTAAACCTTT TGTGGAAAGC TATCAGATAA TTTGCAAGTA CCTTTTGAGT 
GAAGAAGAGG ACCACTTCAG TGAGGAACAG TACTTGGCTG CAGTCAGAAA ATTCACAAGT 
CAGCTTCTCG ATCAAGGTAC CTCTCAATGT TATGATGTAT TATCTTCTGA TGTGCAGAAA 
AACGCCTTAG CAGCCTGTGT GAGGCTCGGA GTAGTGGAGA AGAAGAAGAT AAATAATAAC 
TGTATATTTA ATGTGAATGA ACCTGCCACA ACCAAATTAG AAGAAATGCT TGGTTGTAAG 
ACACCAATAG GAAAACCAGC CACTGCAAAA CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542606.1
CDS	402..2117
Translation	MSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

Target ORF information:

RefSeq Version	XM_011544304.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544304.2

ORF Insert Sequence:

ATGAGTCACA AACTGCGTCT TGGAGCCATT CGGTTTTGTG CCTTCACCCT GAGCAAAGTA 
TTTAAACAAA TTTTCTCGAA GGTGTGTGTA AATGAAGAAG GTATTCAGAA ACTACAAAGA 
GCCATCCAGG AGCATCCTGT TGTTCTGCTG CCTAGTCATC GAAGTTACAT TGACTTCCTC 
ATGTTGTCTT TTCTTCTATA CAATTATGAT TTGCCTGTGC CAGTTATAGC AGCAGGAATG 
GACTTCCTGG GAATGAAAAT GGTTGGTGAG CTGCTACGAA TGTCGGGTGC CTTTTTCATG 
CGGCGTACCT TTGGTGGCAA TAAACTCTAC TGGGCTGTAT TCTCTGAATA TGTAAAAACT 
ATGTTACGGA ATGGTTATGC TCCTGTTGAA TTTTTCCTCG AAGGGACAAG AAGCCGCTCT 
GCCAAGACAT TGACTCCTAA ATTTGGTCTT CTGAATATTG TGATGGAGCC ATTTTTTAAA 
AGAGAAGTTT TTGATACCTA CCTTGTCCCA ATTAGTATCA GTTATGATAA GATCTTGGAA 
GAAACTCTTT ATGTGTATGA GCTTCTAGGG GTTCCTAAAC CAAAAGAGTC TACAACTGGG 
TTGCTGAAAG CCAGAAAGAT TCTCTCTGAA AATTTTGGAA GCATCCATGT GTACTTTGGA 
GATCCTGTGT CACTTCGATC TTTGGCAGCT GGGAGGATGA GTCGGAGCTC ATATAACTTG 
GTTCCAAGAT ACATTCCTCA GAAACAGTCT GAGGACATGC ATGCCTTTGT CACTGAAGTT 
GCCTACAAAA TGGAGCTTCT GCAAATTGAA AACATGGTTT TGAGCCCCTG GACCCTAATA 
GTTGCTGTTC TGCTTCAGAA CCGGCCATCC ATGGACTTTG ATGCTCTGGT GGAAAAGACT 
TTATGGCTAA AAGGCTTAAC CCAGGCATTT GGAGGGTTTC TCATTTGGCC TGATAATAAA 
CCTGCTGAAG AAGTTGTCCC GGCCAGCATT CTTCTGCATT CCAACATTGC CAGCCTTGTC 
AAAGACCAGG TGATTCTGAA AGTGGACTCC GGAGACTCGG AAGTGGTCGA TGGGCTTATG 
CTCCAGCACA TCACTCTCCT CATGTGCTCA GCTTATAGGA ACCAGCTGCT CAACATTTTT 
GTGCGCCCAT CCTTAGTAGC AGTAGCATTG CAGATGACAC CAGGGTTCAG GAAAGAGGAT 
GTCTACAGTT GCTTTCGCTT CCTACGTGAT GTTTTTGCAG ATGAGTTCAT CTTCCTTCCA 
GGAAACACAC TAAAGGACTT TGAAGAAGGC TGTTACCTGC TTTGTAAAAG TGAAGCCATA 
CAAGTGACTA CGAAAGACAT CCTAGTTACA GAGAAAGGAA ATACTGTGTT AGAATTTTTA 
GTAGGACTCT TTAAACCTTT TGTGGAAAGC TATCAGATAA TTTGCAAGTA CCTTTTGAGT 
GAAGAAGAGG ACCACTTCAG TGAGGAACAG TACTTGGCTG CAGTCAGAAA ATTCACAAGT 
CAGCTTCTCG ATCAAGGTAC CTCTCAATGT TATGATGTAT TATCTTCTGA TGTGCAGAAA 
AACGCCTTAG CAGCCTGTGT GAGGCTCGGA GTAGTGGAGA AGAAGAAGAT AAATAATAAC 
TGTATATTTA ATGTGAATGA ACCTGCCACA ACCAAATTAG AAGAAATGCT TGGTTGTAAG 
ACACCAATAG GAAAACCAGC CACTGCAAAA CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNPAT ( XM_005273313.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273370.1 Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu35135
Clone ID Related Accession (Same CDS sequence)	XM_005273313.4
Accession Version	XM_005273313.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dihydroxyacetone phosphate acyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273313.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGGA GCTCAAAAAG TGGGATGAGT TTGAAGATAT TTTAGAAGAG 
AGGAGGCATG TCAGTGACTT GAAATTTGCA ATGAAATGCT ACACACCTCT TGTCTATAAG 
GGAATTACTC CATGTAAACC AATTGATATT AAATGTAGTG TTCTCAATTC TGAGGAGATT 
CATTATGTCA TTAAACAGCT TTCCAAGGAA TCCCTTCAAT CTGTGGATGT CCTCCGAGAG 
GAAGTGAGTG AGATCTTAGA TGAAATGAGT CACAAACTGC GTCTTGGAGC CATTCGGTTT 
TGTGCCTTCA CCCTGAGCAA AGTATTTAAA CAAATTTTCT CGAAGGTGTG TGTAAATGAA 
GAAGGTATTC AGAAACTACA AAGAGCCATC CAGGAGCATC CTGTTGTTCT GCTGCCTAGT 
CATCGAAGTT ACATTGACTT CCTCATGTTG TCTTTTCTTC TATACAATTA TGATTTGCCT 
GTGCCAGTTA TAGCAGCAGG AATGGACTTC CTGGGAATGA AAATGGTTGG TGAGCTGCTA 
CGAATGTCGG GTGCCTTTTT CATGCGGCGT ACCTTTGGTG GCAATAAACT CTACTGGGCT 
GTATTCTCTG AATATGTAAA AACTATGTTA CGGAATGGTT ATGCTCCTGT TGAATTTTTC 
CTCGAAGGGA CAAGAAGCCG CTCTGCCAAG ACATTGACTC CTAAATTTGG TCTTCTGAAT 
ATTGTGATGG AGCCATTTTT TAAAAGAGAA GTTTTTGATA CCTACCTTGT CCCAATTAGT 
ATCAGTTATG ATAAGATCTT GGAAGAAACT CTTTATGTGT ATGAGCTTCT AGGGGTTCCT 
AAACCAAAAG AGTCTACAAC TGGGTTGCTG AAAGCCAGAA AGATTCTCTC TGAAAATTTT 
GGAAGCATCC ATGTGTACTT TGGAGATCCT GTGTCACTTC GATCTTTGGC AGCTGGGAGG 
ATGAGTCGGA GCTCATATAA CTTGGTTCCA AGATACATTC CTCAGAAACA GTCTGAGGAC 
ATGCATGCCT TTGTCACTGA AGTTGCCTAC AAAATGGAGC TTCTGCAAAT TGAAAACATG 
GTTTTGAGCC CCTGGACCCT AATAGTTGCT GTTCTGCTTC AGAACCGGCC ATCCATGGAC 
TTTGATGCTC TGGTGGAAAA GACTTTATGG CTAAAAGGCT TAACCCAGGC ATTTGGAGGG 
TTTCTCATTT GGCCTGATAA TAAACCTGCT GAAGAAGTTG TCCCGGCCAG CATTCTTCTG 
CATTCCAACA TTGCCAGCCT TGTCAAAGAC CAGGTGATTC TGAAAGTGGA CTCCGGAGAC 
TCGGAAGTGG TCGATGGGCT TATGCTCCAG CACATCACTC TCCTCATGTG CTCAGCTTAT 
AGGAACCAGC TGCTCAACAT TTTTGTGCGC CCATCCTTAG TAGCAGTAGC ATTGCAGATG 
ACACCAGGGT TCAGGAAAGA GGATGTCTAC AGTTGCTTTC GCTTCCTACG TGATGTTTTT 
GCAGATGAGT TCATCTTCCT TCCAGGAAAC ACACTAAAGG ACTTTGAAGA AGGCTGTTAC 
CTGCTTTGTA AAAGTGAAGC CATACAAGTG ACTACGAAAG ACATCCTAGT TACAGAGAAA 
GGAAATACTG TGTTAGAATT TTTAGTAGGA CTCTTTAAAC CTTTTGTGGA AAGCTATCAG 
ATAATTTGCA AGTACCTTTT GAGTGAAGAA GAGGACCACT TCAGTGAGGA ACAGTACTTG 
GCTGCAGTCA GAAAATTCAC AAGTCAGCTT CTCGATCAAG GTACCTCTCA ATGTTATGAT 
GTATTATCTT CTGATGTGCA GAAAAACGCC TTAGCAGCCT GTGTGAGGCT CGGAGTAGTG 
GAGAAGAAGA AGATAAATAA TAACTGTATA TTTAATGTGA ATGAACCTGC CACAACCAAA 
TTAGAAGAAA TGCTTGGTTG TAAGACACCA ATAGGAAAAC CAGCCACTGC AAAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273370.1
CDS	170..2209
Translation	MESSSSSNSYFSVGPTSPSAVVLLYSELKKWDEFEDILEERRHVSDLKFAMKCYTPLVYKGITPCKPIDIKCSVLNSEEIHYVIKQLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

Target ORF information:

RefSeq Version	XM_005273313.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glyceronephosphate O-acyltransferase (GNPAT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273313.4

ORF Insert Sequence:

ATGGAGTCTT CCAGTTCATC TAACTCTTAT TTCTCCGTTG GCCCAACCAG TCCCAGCGCT 
GTCGTGCTCC TCTACTCGGA GCTCAAAAAG TGGGATGAGT TTGAAGATAT TTTAGAAGAG 
AGGAGGCATG TCAGTGACTT GAAATTTGCA ATGAAATGCT ACACACCTCT TGTCTATAAG 
GGAATTACTC CATGTAAACC AATTGATATT AAATGTAGTG TTCTCAATTC TGAGGAGATT 
CATTATGTCA TTAAACAGCT TTCCAAGGAA TCCCTTCAAT CTGTGGATGT CCTCCGAGAG 
GAAGTGAGTG AGATCTTAGA TGAAATGAGT CACAAACTGC GTCTTGGAGC CATTCGGTTT 
TGTGCCTTCA CCCTGAGCAA AGTATTTAAA CAAATTTTCT CGAAGGTGTG TGTAAATGAA 
GAAGGTATTC AGAAACTACA AAGAGCCATC CAGGAGCATC CTGTTGTTCT GCTGCCTAGT 
CATCGAAGTT ACATTGACTT CCTCATGTTG TCTTTTCTTC TATACAATTA TGATTTGCCT 
GTGCCAGTTA TAGCAGCAGG AATGGACTTC CTGGGAATGA AAATGGTTGG TGAGCTGCTA 
CGAATGTCGG GTGCCTTTTT CATGCGGCGT ACCTTTGGTG GCAATAAACT CTACTGGGCT 
GTATTCTCTG AATATGTAAA AACTATGTTA CGGAATGGTT ATGCTCCTGT TGAATTTTTC 
CTCGAAGGGA CAAGAAGCCG CTCTGCCAAG ACATTGACTC CTAAATTTGG TCTTCTGAAT 
ATTGTGATGG AGCCATTTTT TAAAAGAGAA GTTTTTGATA CCTACCTTGT CCCAATTAGT 
ATCAGTTATG ATAAGATCTT GGAAGAAACT CTTTATGTGT ATGAGCTTCT AGGGGTTCCT 
AAACCAAAAG AGTCTACAAC TGGGTTGCTG AAAGCCAGAA AGATTCTCTC TGAAAATTTT 
GGAAGCATCC ATGTGTACTT TGGAGATCCT GTGTCACTTC GATCTTTGGC AGCTGGGAGG 
ATGAGTCGGA GCTCATATAA CTTGGTTCCA AGATACATTC CTCAGAAACA GTCTGAGGAC 
ATGCATGCCT TTGTCACTGA AGTTGCCTAC AAAATGGAGC TTCTGCAAAT TGAAAACATG 
GTTTTGAGCC CCTGGACCCT AATAGTTGCT GTTCTGCTTC AGAACCGGCC ATCCATGGAC 
TTTGATGCTC TGGTGGAAAA GACTTTATGG CTAAAAGGCT TAACCCAGGC ATTTGGAGGG 
TTTCTCATTT GGCCTGATAA TAAACCTGCT GAAGAAGTTG TCCCGGCCAG CATTCTTCTG 
CATTCCAACA TTGCCAGCCT TGTCAAAGAC CAGGTGATTC TGAAAGTGGA CTCCGGAGAC 
TCGGAAGTGG TCGATGGGCT TATGCTCCAG CACATCACTC TCCTCATGTG CTCAGCTTAT 
AGGAACCAGC TGCTCAACAT TTTTGTGCGC CCATCCTTAG TAGCAGTAGC ATTGCAGATG 
ACACCAGGGT TCAGGAAAGA GGATGTCTAC AGTTGCTTTC GCTTCCTACG TGATGTTTTT 
GCAGATGAGT TCATCTTCCT TCCAGGAAAC ACACTAAAGG ACTTTGAAGA AGGCTGTTAC 
CTGCTTTGTA AAAGTGAAGC CATACAAGTG ACTACGAAAG ACATCCTAGT TACAGAGAAA 
GGAAATACTG TGTTAGAATT TTTAGTAGGA CTCTTTAAAC CTTTTGTGGA AAGCTATCAG 
ATAATTTGCA AGTACCTTTT GAGTGAAGAA GAGGACCACT TCAGTGAGGA ACAGTACTTG 
GCTGCAGTCA GAAAATTCAC AAGTCAGCTT CTCGATCAAG GTACCTCTCA ATGTTATGAT 
GTATTATCTT CTGATGTGCA GAAAAACGCC TTAGCAGCCT GTGTGAGGCT CGGAGTAGTG 
GAGAAGAAGA AGATAAATAA TAACTGTATA TTTAATGTGA ATGAACCTGC CACAACCAAA 
TTAGAAGAAA TGCTTGGTTG TAAGACACCA ATAGGAAAAC CAGCCACTGC AAAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GNPAT rs11558492 is not a Major Modifier of Iron Status: Study of Italian Hemochromatosis Patients and Blood Donors.
Annals of hepatology16(3)451-456(2017 May - Jun)
Greni F,Valenti L,Mariani R,Pelloni I,Rametta R,Busti F,Ravasi G,Girelli D,Fargion S,Galimberti S,Piperno A,Pelucchi S

Reduction of Ether-Type Glycerophospholipids, Plasmalogens, by NF-?B Signal Leading to Microglial Activation.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(15)4074-4092(2017 Apr)
Hossain MS,Abe Y,Ali F,Youssef M,Honsho M,Fujiki Y,Katafuchi T

GNPAT p.D519G is independently associated with markedly increased iron stores in HFE p.C282Y homozygotes.
Blood cells, molecules & diseases6315-20(2017 Mar)
Barton JC,Chen WP,Emond MJ,Phatak PD,Subramaniam VN,Adams PC,Gurrin LC,Anderson GJ,Ramm GA,Powell LW,Allen KJ,Phillips JD,Parker CJ,McLaren GD,McLaren CE

Recombinant human dihydroxyacetonephosphate acyl-transferase characterization as an integral monotopic membrane protein.
Biochemical and biophysical research communications481(1-2)51-58(2016 Dec)
Piano V,Nenci S,Magnani F,Aliverti A,Mattevi A

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GNPAT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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