RAD54L cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD54L
Entrez Gene ID	8438
Full Name	RAD54 like
Synonyms	HR54,RAD54A,hHR54,hRAD54
General protein information	Preferred Names RAD54 like Names DNA repair and recombination protein RAD54-like RAD54 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.1
Summary	The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008].
Disorder MIM:	603615
Disorder Html:	{Breast cancer, invasive ductal}, 114480 (3); Lymphoma, non-Hodgkin, somatic, 605027 (3); Adenocarcinoma, colonic, somatic (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001370766.1	NP_001357695.1	DNA repair and recombination protein RAD54-like isoform 2
NM_001142548.1	NP_001136020.1	DNA repair and recombination protein RAD54-like isoform 1
NM_003579.4	NP_003570.2	DNA repair and recombination protein RAD54-like isoform 1
XM_011542299.2	XP_011540601.1	DNA repair and recombination protein RAD54-like isoform X2
XM_011542300.3	XP_011540602.1	DNA repair and recombination protein RAD54-like isoform X2
XM_011542300.2	XP_011540602.1	DNA repair and recombination protein RAD54-like isoform X2
NM_003579.3	NP_003570.2	DNA repair and recombination protein RAD54-like
XM_006710975.3	XP_006711038.1	DNA repair and recombination protein RAD54-like isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	rad54l	NP_957438.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008748	XP_557901.3
Arabidopsis thaliana (thale cress)	RAD54	NP_188552.3
Xenopus tropicalis (tropical clawed frog)	rad54l	NP_001001241.1
Pan troglodytes (chimpanzee)	RAD54L	XP_513146.3
Bos taurus (cattle)	RAD54L	NP_001124238.1
Mus musculus (house mouse)	Rad54l	NP_033041.3
Gallus gallus (chicken)	RAD54L	XP_422447.3
Caenorhabditis elegans (roundworm)	rad-54	NP_492438.1
Saccharomyces cerevisiae (baker's yeast)	RAD54	NP_011352.1
Kluyveromyces lactis	KLLA0A03069g	XP_451134.1
Eremothecium gossypii	AGOS_AEL297W	NP_984563.2
Drosophila melanogaster (fruit fly)	okr	NP_476661.1
Magnaporthe oryzae (rice blast fungus)	MGG_03549	XP_003716366.1
Neurospora crassa	NCU11255	XP_959470.1
Oryza sativa (rice)	Os02g0762800	NP_001048207.2
Homo sapiens (human)	RAD54L	NP_003570.2
Canis lupus familiaris (dog)	RAD54L	XP_532592.3
Rattus norvegicus (Norway rat)	Rad54l	NP_001128432.1
Schizosaccharomyces pombe (fission yeast)	rhp54	NP_594290.1

Gene Ontology
Bibliography

Related articles in PubMed

RAD54 N-terminal domain is a DNA sensor that couples ATP hydrolysis with branch migration of Holliday junctions.
Goyal N, Rossi MJ, Mazina OM, Chi Y, Moritz RL, Clurman BE, Mazin AV
Nature communications9(1)34(2018 01)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Cross-Species Genomics Identifies TAF12, NFYC, and RAD54L as Choroid Plexus Carcinoma Oncogenes.
Tong Y, Merino D, Nimmervoll B, Gupta K, Wang YD, Finkelstein D, Dalton J, Ellison DW, Ma X, Zhang J, Malkin D, Gilbertson RJ
Cancer cell27(5)712-27(2015 May)

RAD54 family translocases counter genotoxic effects of RAD51 in human tumor cells.
Mason JM, Dusad K, Wright WD, Grubb J, Budke B, Heyer WD, Connell PP, Weichselbaum RR, Bishop DK
Nucleic acids research43(6)3180-96(2015 Mar)

Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1.
Machida S, Takaku M, Ikura M, Sun J, Suzuki H, Kobayashi W, Kinomura A, Osakabe A, Tachiwana H, Horikoshi Y, Fukuto A, Matsuda R, Ura K, Tashiro S, Ikura T, Kurumizaka H
Scientific reports44863(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TAF12 and NFYC are transcription factors that regulate the epigenome, whereas RAD54L plays a central role in DNA repair
Title: Cross-Species Genomics Identifies TAF12, NFYC, and RAD54L as Choroid Plexus Carcinoma Oncogenes.

support a model in which RAD54L and RAD54B counteract genome-destabilizing effects of direct binding of RAD51 to dsDNA in tumor cells
Title: RAD54 family translocases counter genotoxic effects of RAD51 in human tumor cells.

Nap1 binds to RAD54.
Title: Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1.

Meta-analysis of gene-disease association. (HuGE Navigator)
Title: Polymorphisms in DNA repair genes and risk of non-Hodgkin lymphoma in a pooled analysis of three studies.

Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: Gamma-radiation sensitivity and polymorphisms in RAD51L1 modulate glioma risk.

The following RAD54L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD54L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03403	NM_001142548.1 Latest version!	Homo sapiens RAD54 like (RAD54L), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu03403	NM_003579.4 Latest version!	Homo sapiens RAD54 like (RAD54L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu33903	NM_001370766.1 Latest version!	Homo sapiens RAD54 like (RAD54L), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu33903	XM_006710975.3 Latest version!	Homo sapiens RAD54 like (RAD54L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu61771	XM_011542300.3 Latest version!	Homo sapiens RAD54 like (RAD54L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu61771	XM_011542299.2 Latest version!	Homo sapiens RAD54 like (RAD54L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu61771	XM_011542300.2	Homo sapiens RAD54-like (S. cerevisiae) (RAD54L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu03403	NM_003579.3	Homo sapiens RAD54 like (RAD54L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD54L ( NM_001142548.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136020.1 Homo sapiens RAD54 like (RAD54L), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03403
Clone ID Related Accession (Same CDS sequence)	NM_001142548.1 , NM_003579.4 , NM_003579.3
Accession Version	NM_001142548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	DNA repair and recombination protein RAD54-like isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB048280.1, AK292611.1, BC121060.1 and BM678712.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292611.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGGA GCTTGGCTCC CAGCCAGCTG GCCAAGAGAA AACCTGAAGG CAGGTCCTGT 
GATGATGAAG ACTGGCAACC TGGCCTAGTG ACTCCTAGGA AACGGAAATC CAGCAGTGAG 
ACCCAGATCC AGGAGTGTTT CCTGTCTCCT TTTCGGAAAC CTTTGAGTCA GCTAACCAAT 
CAACCACCTT GTCTGGACAG CAGTCAGCAT GAAGCATTTA TTCGAAGCAT TTTGTCAAAG 
CCTTTCAAAG TCCCCATTCC AAATTATCAA GGTCCTCTGG GCTCTCGAGC ATTGGGCCTG 
AAAAGGGCTG GGGTCCGCCG GGCCCTCCAT GACCCCCTGG AAAAAGATGC CTTGGTTCTG 
TATGAGCCTC CCCCGCTGAG CGCTCATGAC CAGCTGAAGC TTGACAAGGA GAAACTCCCT 
GTCCATGTGG TTGTTGACCC TATTCTCAGT AAGGTTTTGC GGCCTCATCA GAGAGAGGGA 
GTGAAATTCC TGTGGGAGTG TGTCACCAGT CGGCGCATCC CTGGCAGCCA TGGCTGCATC 
ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136020.1
CDS	115..2358
Translation	MRRSLAPSQLAKRKPEGRSCDDEDWQPGLVTPRKRKSSSETQIQECFLSPFRKPLSQLTNQPPCLDSSQHEAFIRSILSKPFKVPIPNYQGPLGSRALGLKRAGVRRALHDPLEKDALVLYEPPPLSAHDQLKLDKEKLPVHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPEIDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Target ORF information:

RefSeq Version	NM_001142548.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD54 like (RAD54L), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142548.1

ORF Insert Sequence:

ATGAGGAGGA GCTTGGCTCC CAGCCAGCTG GCCAAGAGAA AACCTGAAGG CAGGTCCTGT 
GATGATGAAG ACTGGCAACC TGGCCTAGTG ACTCCTAGGA AACGGAAATC CAGCAGTGAG 
ACCCAGATCC AGGAGTGTTT CCTGTCTCCT TTTCGGAAAC CTTTGAGTCA GCTAACCAAT 
CAACCACCTT GTCTGGACAG CAGTCAGCAT GAAGCATTTA TTCGAAGCAT TTTGTCAAAG 
CCTTTCAAAG TCCCCATTCC AAATTATCAA GGTCCTCTGG GCTCTCGAGC ATTGGGCCTG 
AAAAGGGCTG GGGTCCGCCG GGCCCTCCAT GACCCCCTGG AAAAAGATGC CTTGGTTCTG 
TATGAGCCTC CCCCGCTGAG CGCTCATGAC CAGCTGAAGC TTGACAAGGA GAAACTCCCT 
GTCCATGTGG TTGTTGACCC TATTCTCAGT AAGGTTTTGC GGCCTCATCA GAGAGAGGGA 
GTGAAATTCC TGTGGGAGTG TGTCACCAGT CGGCGCATCC CTGGCAGCCA TGGCTGCATC 
ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD54L ( NM_003579.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003570.2 Homo sapiens RAD54 like (RAD54L), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03403
Clone ID Related Accession (Same CDS sequence)	NM_001142548.1 , NM_003579.4 , NM_003579.3
Accession Version	NM_003579.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	DNA repair and recombination protein RAD54-like isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB449264.2, BC121060.1 and BM678712.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_003579.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC121059.1, X97795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371975.9/ ENSP00000361043.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGAGGA GCTTGGCTCC CAGCCAGCTG GCCAAGAGAA AACCTGAAGG CAGGTCCTGT 
GATGATGAAG ACTGGCAACC TGGCCTAGTG ACTCCTAGGA AACGGAAATC CAGCAGTGAG 
ACCCAGATCC AGGAGTGTTT CCTGTCTCCT TTTCGGAAAC CTTTGAGTCA GCTAACCAAT 
CAACCACCTT GTCTGGACAG CAGTCAGCAT GAAGCATTTA TTCGAAGCAT TTTGTCAAAG 
CCTTTCAAAG TCCCCATTCC AAATTATCAA GGTCCTCTGG GCTCTCGAGC ATTGGGCCTG 
AAAAGGGCTG GGGTCCGCCG GGCCCTCCAT GACCCCCTGG AAAAAGATGC CTTGGTTCTG 
TATGAGCCTC CCCCGCTGAG CGCTCATGAC CAGCTGAAGC TTGACAAGGA GAAACTCCCT 
GTCCATGTGG TTGTTGACCC TATTCTCAGT AAGGTTTTGC GGCCTCATCA GAGAGAGGGA 
GTGAAATTCC TGTGGGAGTG TGTCACCAGT CGGCGCATCC CTGGCAGCCA TGGCTGCATC 
ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003570.2
CDS	644..2887
Translation	MRRSLAPSQLAKRKPEGRSCDDEDWQPGLVTPRKRKSSSETQIQECFLSPFRKPLSQLTNQPPCLDSSQHEAFIRSILSKPFKVPIPNYQGPLGSRALGLKRAGVRRALHDPLEKDALVLYEPPPLSAHDQLKLDKEKLPVHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPEIDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Target ORF information:

RefSeq Version	NM_003579.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD54 like (RAD54L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003579.4

ORF Insert Sequence:

ATGAGGAGGA GCTTGGCTCC CAGCCAGCTG GCCAAGAGAA AACCTGAAGG CAGGTCCTGT 
GATGATGAAG ACTGGCAACC TGGCCTAGTG ACTCCTAGGA AACGGAAATC CAGCAGTGAG 
ACCCAGATCC AGGAGTGTTT CCTGTCTCCT TTTCGGAAAC CTTTGAGTCA GCTAACCAAT 
CAACCACCTT GTCTGGACAG CAGTCAGCAT GAAGCATTTA TTCGAAGCAT TTTGTCAAAG 
CCTTTCAAAG TCCCCATTCC AAATTATCAA GGTCCTCTGG GCTCTCGAGC ATTGGGCCTG 
AAAAGGGCTG GGGTCCGCCG GGCCCTCCAT GACCCCCTGG AAAAAGATGC CTTGGTTCTG 
TATGAGCCTC CCCCGCTGAG CGCTCATGAC CAGCTGAAGC TTGACAAGGA GAAACTCCCT 
GTCCATGTGG TTGTTGACCC TATTCTCAGT AAGGTTTTGC GGCCTCATCA GAGAGAGGGA 
GTGAAATTCC TGTGGGAGTG TGTCACCAGT CGGCGCATCC CTGGCAGCCA TGGCTGCATC 
ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD54L ( NM_003579.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003570.2 Homo sapiens RAD54 like (RAD54L), transcript variant 1, mRNA.

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CloneID	OHu03403
Clone ID Related Accession (Same CDS sequence)	NM_001142548.1 , NM_003579.4 , NM_003579.3
Accession Version	NM_003579.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	DNA repair and recombination protein RAD54-like
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB048280.1, DB449264.2, BC121060.1 and BM678712.1. On Dec 9, 2008 this sequence version replaced NM_003579.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC121059.1, X97795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGGA GCTTGGCTCC CAGCCAGCTG GCCAAGAGAA AACCTGAAGG CAGGTCCTGT 
GATGATGAAG ACTGGCAACC TGGCCTAGTG ACTCCTAGGA AACGGAAATC CAGCAGTGAG 
ACCCAGATCC AGGAGTGTTT CCTGTCTCCT TTTCGGAAAC CTTTGAGTCA GCTAACCAAT 
CAACCACCTT GTCTGGACAG CAGTCAGCAT GAAGCATTTA TTCGAAGCAT TTTGTCAAAG 
CCTTTCAAAG TCCCCATTCC AAATTATCAA GGTCCTCTGG GCTCTCGAGC ATTGGGCCTG 
AAAAGGGCTG GGGTCCGCCG GGCCCTCCAT GACCCCCTGG AAAAAGATGC CTTGGTTCTG 
TATGAGCCTC CCCCGCTGAG CGCTCATGAC CAGCTGAAGC TTGACAAGGA GAAACTCCCT 
GTCCATGTGG TTGTTGACCC TATTCTCAGT AAGGTTTTGC GGCCTCATCA GAGAGAGGGA 
GTGAAATTCC TGTGGGAGTG TGTCACCAGT CGGCGCATCC CTGGCAGCCA TGGCTGCATC 
ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003570.2
CDS	712..2955
Translation	MRRSLAPSQLAKRKPEGRSCDDEDWQPGLVTPRKRKSSSETQIQECFLSPFRKPLSQLTNQPPCLDSSQHEAFIRSILSKPFKVPIPNYQGPLGSRALGLKRAGVRRALHDPLEKDALVLYEPPPLSAHDQLKLDKEKLPVHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPEIDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Target ORF information:

RefSeq Version	NM_003579.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD54 like (RAD54L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003579.3

ORF Insert Sequence:

ATGAGGAGGA GCTTGGCTCC CAGCCAGCTG GCCAAGAGAA AACCTGAAGG CAGGTCCTGT 
GATGATGAAG ACTGGCAACC TGGCCTAGTG ACTCCTAGGA AACGGAAATC CAGCAGTGAG 
ACCCAGATCC AGGAGTGTTT CCTGTCTCCT TTTCGGAAAC CTTTGAGTCA GCTAACCAAT 
CAACCACCTT GTCTGGACAG CAGTCAGCAT GAAGCATTTA TTCGAAGCAT TTTGTCAAAG 
CCTTTCAAAG TCCCCATTCC AAATTATCAA GGTCCTCTGG GCTCTCGAGC ATTGGGCCTG 
AAAAGGGCTG GGGTCCGCCG GGCCCTCCAT GACCCCCTGG AAAAAGATGC CTTGGTTCTG 
TATGAGCCTC CCCCGCTGAG CGCTCATGAC CAGCTGAAGC TTGACAAGGA GAAACTCCCT 
GTCCATGTGG TTGTTGACCC TATTCTCAGT AAGGTTTTGC GGCCTCATCA GAGAGAGGGA 
GTGAAATTCC TGTGGGAGTG TGTCACCAGT CGGCGCATCC CTGGCAGCCA TGGCTGCATC 
ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD54L ( NM_001370766.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357695.1 Homo sapiens RAD54 like (RAD54L), transcript variant 3, mRNA.

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CloneID	OHu33903
Clone ID Related Accession (Same CDS sequence)	NM_001370766.1 , XM_006710975.3
Accession Version	NM_001370766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	DNA repair and recombination protein RAD54-like isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121602.34. On May 29, 2019 this sequence version replaced XM_006710975.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1001425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357695.1
CDS	597..2300
Translation	MADEMGLGKTLQCITLMWTLLRQSPECKPEIDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Target ORF information:

RefSeq Version	NM_001370766.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD54 like (RAD54L), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370766.1

ORF Insert Sequence:

ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD54L ( XM_006710975.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711038.1 Homo sapiens RAD54 like (RAD54L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu33903
Clone ID Related Accession (Same CDS sequence)	NM_001370766.1 , XM_006710975.3
Accession Version	XM_006710975.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	DNA repair and recombination protein RAD54-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710975.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711038.1
CDS	590..2293
Translation	MADEMGLGKTLQCITLMWTLLRQSPECKPEIDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Target ORF information:

RefSeq Version	XM_006710975.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD54 like (RAD54L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710975.3

ORF Insert Sequence:

ATGGCTGATG AGATGGGCCT AGGAAAGACG CTGCAGTGCA TCACATTGAT GTGGACACTT 
TTACGCCAGA GTCCAGAGTG CAAGCCAGAA ATTGACAAGG CAGTGGTGGT GTCGCCTTCC 
AGCCTGGTGA AGAACTGGTA CAATGAGGTT GGGAAATGGC TCGGAGGGAG GATCCAACCT 
CTGGCCATCG ATGGAGGATC TAAGGATGAA ATAGACCAAA AGCTGGAAGG ATTCATGAAC 
CAGCGTGGAG CCAGGGTGTC TTCTCCCATC CTCATCATTT CCTATGAGAC CTTCCGCCTT 
CATGTTGGAG TCCTCCAGAA AGGAAGTGTT GGTCTGGTCA TATGTGACGA GGGACACAGG 
CTCAAGAACT CTGAGAATCA GACTTACCAA GCCCTGGACA GCTTGAACAC CAGCCGGCGG 
GTGCTCATCT CCGGAACTCC CATCCAGAAT GATCTGCTTG AGTATTTCAG CTTGGTACAT 
TTTGTTAATT CCGGCATCCT AGGGACTGCC CATGAATTCA AGAAGCATTT TGAATTGCCA 
ATTTTGAAGG GTCGAGACGC TGCTGCTAGT GAGGCAGACA GGCAGCTAGG AGAGGAGCGG 
CTGCGGGAGC TCACCAGCAT TGTGAATAGA TGCCTGATAC GGAGGACTTC TGATATCCTT 
TCTAAATATC TGCCTGTGAA GATTGAGCAG GTCGTTTGTT GTAGGCTGAC ACCCCTTCAG 
ACTGAGTTAT ACAAGAGGTT TCTGAGACAA GCCAAACCGG CAGAAGAATT GCTTGAGGGC 
AAGATGAGTG TGTCTTCCCT TTCTTCCATC ACCTCGCTAA AGAAGCTTTG TAATCATCCA 
GCTCTAATCT ATGATAAGTG TGTGGAAGAG GAGGATGGCT TTGTGGGTGC CTTGGACCTC 
TTCCCTCCTG GTTACAGCTC TAAGGCCCTG GAGCCCCAGC TGTCAGGTAA GATGCTGGTC 
CTGGATTATA TTCTGGCGGT GACCCGAAGC CGTAGCAGTG ACAAAGTAGT GCTGGTGTCG 
AATTACACCC AGACTTTGGA TCTCTTTGAG AAGCTGTGCC GTGCCCGAAG GTACTTATAC 
GTCCGCCTGG ATGGCACGAT GTCCATTAAG AAGCGAGCCA AGGTTGTAGA ACGCTTCAAT 
AGTCCATCGA GCCCTGACTT TGTCTTCATG CTGAGCAGCA AAGCTGGGGG CTGTGGCCTC 
AATCTCATTG GGGCTAACCG GCTGGTCATG TTTGACCCTG ACTGGAACCC AGCCAATGAT 
GAACAAGCCA TGGCCCGGGT CTGGCGAGAT GGTCAAAAGA AGACTTGCTA TATCTACCGC 
CTGCTGTCTG CAGGGACCAT TGAGGAGAAG ATCTTCCAGC GTCAGAGCCA CAAGAAGGCA 
CTGAGCAGCT GTGTGGTGGA TGAGGAGCAG GATGTAGAGC GCCACTTCTC TCTGGGCGAG 
TTGAAGGAGC TGTTTATCCT GGATGAAGCT AGCCTCAGTG ACACACATGA CAGGTTGCAC 
TGCCGACGTT GTGTCAACAG CCGTCAGATC CGGCCACCCC CTGATGGTTC TGACTGCACT 
TCAGACCTGG CAGGGTGGAA CCACTGCACT GATAAGTGGG GGCTCCGGGA TGAGGTACTC 
CAGGCTGCCT GGGATGCTGC CTCCACTGCC ATCACCTTCG TCTTCCACCA GCGTTCTCAT 
GAGGAGCAGC GGGGCCTCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD54L ( XM_011542299.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540601.1 Homo sapiens RAD54 like (RAD54L), transcript variant X2, mRNA.

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CloneID	OHu61771
Clone ID Related Accession (Same CDS sequence)	XM_011542299.2 , XM_011542300.3 , XM_011542300.2
Accession Version	XM_011542299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA repair and recombination protein RAD54-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGC GTGGAGCCAG GGTGTCTTCT CCCATCCTCA TCATTTCCTA TGAGACCTTC 
CGCCTTCATG TTGGAGTCCT CCAGAAAGGA AGTGTTGGTC TGGTCATATG TGACGAGGGA 
CACAGGCTCA AGAACTCTGA GAATCAGACT TACCAAGCCC TGGACAGCTT GAACACCAGC 
CGGCGGGTGC TCATCTCCGG AACTCCCATC CAGAATGATC TGCTTGAGTA TTTCAGCTTG 
GTACATTTTG TTAATTCCGG CATCCTAGGG ACTGCCCATG AATTCAAGAA GCATTTTGAA 
TTGCCAATTT TGAAGGGTCG AGACGCTGCT GCTAGTGAGG CAGACAGGCA GCTAGGAGAG 
GAGCGGCTGC GGGAGCTCAC CAGCATTGTG AATAGATGCC TGATACGGAG GACTTCTGAT 
ATCCTTTCTA AATATCTGCC TGTGAAGATT GAGCAGGTCG TTTGTTGTAG GCTGACACCC 
CTTCAGACTG AGTTATACAA GAGGTTTCTG AGACAAGCCA AACCGGCAGA AGAATTGCTT 
GAGGGCAAGA TGAGTGTGTC TTCCCTTTCT TCCATCACCT CGCTAAAGAA GCTTTGTAAT 
CATCCAGCTC TAATCTATGA TAAGTGTGTG GAAGAGGAGG ATGGCTTTGT GGGTGCCTTG 
GACCTCTTCC CTCCTGGTTA CAGCTCTAAG GCCCTGGAGC CCCAGCTGTC AGGTAAGATG 
CTGGTCCTGG ATTATATTCT GGCGGTGACC CGAAGCCGTA GCAGTGACAA AGTAGTGCTG 
GTGTCGAATT ACACCCAGAC TTTGGATCTC TTTGAGAAGC TGTGCCGTGC CCGAAGGTAC 
TTATACGTCC GCCTGGATGG CACGATGTCC ATTAAGAAGC GAGCCAAGGT TGTAGAACGC 
TTCAATAGTC CATCGAGCCC TGACTTTGTC TTCATGCTGA GCAGCAAAGC TGGGGGCTGT 
GGCCTCAATC TCATTGGGGC TAACCGGCTG GTCATGTTTG ACCCTGACTG GAACCCAGCC 
AATGATGAAC AAGCCATGGC CCGGGTCTGG CGAGATGGTC AAAAGAAGAC TTGCTATATC 
TACCGCCTGC TGTCTGCAGG GACCATTGAG GAGAAGATCT TCCAGCGTCA GAGCCACAAG 
AAGGCACTGA GCAGCTGTGT GGTGGATGAG GAGCAGGATG TAGAGCGCCA CTTCTCTCTG 
GGCGAGTTGA AGGAGCTGTT TATCCTGGAT GAAGCTAGCC TCAGTGACAC ACATGACAGG 
TTGCACTGCC GACGTTGTGT CAACAGCCGT CAGATCCGGC CACCCCCTGA TGGTTCTGAC 
TGCACTTCAG ACCTGGCAGG GTGGAACCAC TGCACTGATA AGTGGGGGCT CCGGGATGAG 
GTACTCCAGG CTGCCTGGGA TGCTGCCTCC ACTGCCATCA CCTTCGTCTT CCACCAGCGT 
TCTCATGAGG AGCAGCGGGG CCTCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540601.1
CDS	256..1725
Translation	MNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Target ORF information:

RefSeq Version	XM_011542299.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD54 like (RAD54L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542299.2

ORF Insert Sequence:

ATGAACCAGC GTGGAGCCAG GGTGTCTTCT CCCATCCTCA TCATTTCCTA TGAGACCTTC 
CGCCTTCATG TTGGAGTCCT CCAGAAAGGA AGTGTTGGTC TGGTCATATG TGACGAGGGA 
CACAGGCTCA AGAACTCTGA GAATCAGACT TACCAAGCCC TGGACAGCTT GAACACCAGC 
CGGCGGGTGC TCATCTCCGG AACTCCCATC CAGAATGATC TGCTTGAGTA TTTCAGCTTG 
GTACATTTTG TTAATTCCGG CATCCTAGGG ACTGCCCATG AATTCAAGAA GCATTTTGAA 
TTGCCAATTT TGAAGGGTCG AGACGCTGCT GCTAGTGAGG CAGACAGGCA GCTAGGAGAG 
GAGCGGCTGC GGGAGCTCAC CAGCATTGTG AATAGATGCC TGATACGGAG GACTTCTGAT 
ATCCTTTCTA AATATCTGCC TGTGAAGATT GAGCAGGTCG TTTGTTGTAG GCTGACACCC 
CTTCAGACTG AGTTATACAA GAGGTTTCTG AGACAAGCCA AACCGGCAGA AGAATTGCTT 
GAGGGCAAGA TGAGTGTGTC TTCCCTTTCT TCCATCACCT CGCTAAAGAA GCTTTGTAAT 
CATCCAGCTC TAATCTATGA TAAGTGTGTG GAAGAGGAGG ATGGCTTTGT GGGTGCCTTG 
GACCTCTTCC CTCCTGGTTA CAGCTCTAAG GCCCTGGAGC CCCAGCTGTC AGGTAAGATG 
CTGGTCCTGG ATTATATTCT GGCGGTGACC CGAAGCCGTA GCAGTGACAA AGTAGTGCTG 
GTGTCGAATT ACACCCAGAC TTTGGATCTC TTTGAGAAGC TGTGCCGTGC CCGAAGGTAC 
TTATACGTCC GCCTGGATGG CACGATGTCC ATTAAGAAGC GAGCCAAGGT TGTAGAACGC 
TTCAATAGTC CATCGAGCCC TGACTTTGTC TTCATGCTGA GCAGCAAAGC TGGGGGCTGT 
GGCCTCAATC TCATTGGGGC TAACCGGCTG GTCATGTTTG ACCCTGACTG GAACCCAGCC 
AATGATGAAC AAGCCATGGC CCGGGTCTGG CGAGATGGTC AAAAGAAGAC TTGCTATATC 
TACCGCCTGC TGTCTGCAGG GACCATTGAG GAGAAGATCT TCCAGCGTCA GAGCCACAAG 
AAGGCACTGA GCAGCTGTGT GGTGGATGAG GAGCAGGATG TAGAGCGCCA CTTCTCTCTG 
GGCGAGTTGA AGGAGCTGTT TATCCTGGAT GAAGCTAGCC TCAGTGACAC ACATGACAGG 
TTGCACTGCC GACGTTGTGT CAACAGCCGT CAGATCCGGC CACCCCCTGA TGGTTCTGAC 
TGCACTTCAG ACCTGGCAGG GTGGAACCAC TGCACTGATA AGTGGGGGCT CCGGGATGAG 
GTACTCCAGG CTGCCTGGGA TGCTGCCTCC ACTGCCATCA CCTTCGTCTT CCACCAGCGT 
TCTCATGAGG AGCAGCGGGG CCTCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD54L ( XM_011542300.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540602.1 Homo sapiens RAD54 like (RAD54L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu61771
Clone ID Related Accession (Same CDS sequence)	XM_011542299.2 , XM_011542300.3 , XM_011542300.2
Accession Version	XM_011542300.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA repair and recombination protein RAD54-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011542300.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGC GTGGAGCCAG GGTGTCTTCT CCCATCCTCA TCATTTCCTA TGAGACCTTC 
CGCCTTCATG TTGGAGTCCT CCAGAAAGGA AGTGTTGGTC TGGTCATATG TGACGAGGGA 
CACAGGCTCA AGAACTCTGA GAATCAGACT TACCAAGCCC TGGACAGCTT GAACACCAGC 
CGGCGGGTGC TCATCTCCGG AACTCCCATC CAGAATGATC TGCTTGAGTA TTTCAGCTTG 
GTACATTTTG TTAATTCCGG CATCCTAGGG ACTGCCCATG AATTCAAGAA GCATTTTGAA 
TTGCCAATTT TGAAGGGTCG AGACGCTGCT GCTAGTGAGG CAGACAGGCA GCTAGGAGAG 
GAGCGGCTGC GGGAGCTCAC CAGCATTGTG AATAGATGCC TGATACGGAG GACTTCTGAT 
ATCCTTTCTA AATATCTGCC TGTGAAGATT GAGCAGGTCG TTTGTTGTAG GCTGACACCC 
CTTCAGACTG AGTTATACAA GAGGTTTCTG AGACAAGCCA AACCGGCAGA AGAATTGCTT 
GAGGGCAAGA TGAGTGTGTC TTCCCTTTCT TCCATCACCT CGCTAAAGAA GCTTTGTAAT 
CATCCAGCTC TAATCTATGA TAAGTGTGTG GAAGAGGAGG ATGGCTTTGT GGGTGCCTTG 
GACCTCTTCC CTCCTGGTTA CAGCTCTAAG GCCCTGGAGC CCCAGCTGTC AGGTAAGATG 
CTGGTCCTGG ATTATATTCT GGCGGTGACC CGAAGCCGTA GCAGTGACAA AGTAGTGCTG 
GTGTCGAATT ACACCCAGAC TTTGGATCTC TTTGAGAAGC TGTGCCGTGC CCGAAGGTAC 
TTATACGTCC GCCTGGATGG CACGATGTCC ATTAAGAAGC GAGCCAAGGT TGTAGAACGC 
TTCAATAGTC CATCGAGCCC TGACTTTGTC TTCATGCTGA GCAGCAAAGC TGGGGGCTGT 
GGCCTCAATC TCATTGGGGC TAACCGGCTG GTCATGTTTG ACCCTGACTG GAACCCAGCC 
AATGATGAAC AAGCCATGGC CCGGGTCTGG CGAGATGGTC AAAAGAAGAC TTGCTATATC 
TACCGCCTGC TGTCTGCAGG GACCATTGAG GAGAAGATCT TCCAGCGTCA GAGCCACAAG 
AAGGCACTGA GCAGCTGTGT GGTGGATGAG GAGCAGGATG TAGAGCGCCA CTTCTCTCTG 
GGCGAGTTGA AGGAGCTGTT TATCCTGGAT GAAGCTAGCC TCAGTGACAC ACATGACAGG 
TTGCACTGCC GACGTTGTGT CAACAGCCGT CAGATCCGGC CACCCCCTGA TGGTTCTGAC 
TGCACTTCAG ACCTGGCAGG GTGGAACCAC TGCACTGATA AGTGGGGGCT CCGGGATGAG 
GTACTCCAGG CTGCCTGGGA TGCTGCCTCC ACTGCCATCA CCTTCGTCTT CCACCAGCGT 
TCTCATGAGG AGCAGCGGGG CCTCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540602.1
CDS	73..1542
Translation	MNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Target ORF information:

RefSeq Version	XM_011542300.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD54 like (RAD54L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542300.3

ORF Insert Sequence:

ATGAACCAGC GTGGAGCCAG GGTGTCTTCT CCCATCCTCA TCATTTCCTA TGAGACCTTC 
CGCCTTCATG TTGGAGTCCT CCAGAAAGGA AGTGTTGGTC TGGTCATATG TGACGAGGGA 
CACAGGCTCA AGAACTCTGA GAATCAGACT TACCAAGCCC TGGACAGCTT GAACACCAGC 
CGGCGGGTGC TCATCTCCGG AACTCCCATC CAGAATGATC TGCTTGAGTA TTTCAGCTTG 
GTACATTTTG TTAATTCCGG CATCCTAGGG ACTGCCCATG AATTCAAGAA GCATTTTGAA 
TTGCCAATTT TGAAGGGTCG AGACGCTGCT GCTAGTGAGG CAGACAGGCA GCTAGGAGAG 
GAGCGGCTGC GGGAGCTCAC CAGCATTGTG AATAGATGCC TGATACGGAG GACTTCTGAT 
ATCCTTTCTA AATATCTGCC TGTGAAGATT GAGCAGGTCG TTTGTTGTAG GCTGACACCC 
CTTCAGACTG AGTTATACAA GAGGTTTCTG AGACAAGCCA AACCGGCAGA AGAATTGCTT 
GAGGGCAAGA TGAGTGTGTC TTCCCTTTCT TCCATCACCT CGCTAAAGAA GCTTTGTAAT 
CATCCAGCTC TAATCTATGA TAAGTGTGTG GAAGAGGAGG ATGGCTTTGT GGGTGCCTTG 
GACCTCTTCC CTCCTGGTTA CAGCTCTAAG GCCCTGGAGC CCCAGCTGTC AGGTAAGATG 
CTGGTCCTGG ATTATATTCT GGCGGTGACC CGAAGCCGTA GCAGTGACAA AGTAGTGCTG 
GTGTCGAATT ACACCCAGAC TTTGGATCTC TTTGAGAAGC TGTGCCGTGC CCGAAGGTAC 
TTATACGTCC GCCTGGATGG CACGATGTCC ATTAAGAAGC GAGCCAAGGT TGTAGAACGC 
TTCAATAGTC CATCGAGCCC TGACTTTGTC TTCATGCTGA GCAGCAAAGC TGGGGGCTGT 
GGCCTCAATC TCATTGGGGC TAACCGGCTG GTCATGTTTG ACCCTGACTG GAACCCAGCC 
AATGATGAAC AAGCCATGGC CCGGGTCTGG CGAGATGGTC AAAAGAAGAC TTGCTATATC 
TACCGCCTGC TGTCTGCAGG GACCATTGAG GAGAAGATCT TCCAGCGTCA GAGCCACAAG 
AAGGCACTGA GCAGCTGTGT GGTGGATGAG GAGCAGGATG TAGAGCGCCA CTTCTCTCTG 
GGCGAGTTGA AGGAGCTGTT TATCCTGGAT GAAGCTAGCC TCAGTGACAC ACATGACAGG 
TTGCACTGCC GACGTTGTGT CAACAGCCGT CAGATCCGGC CACCCCCTGA TGGTTCTGAC 
TGCACTTCAG ACCTGGCAGG GTGGAACCAC TGCACTGATA AGTGGGGGCT CCGGGATGAG 
GTACTCCAGG CTGCCTGGGA TGCTGCCTCC ACTGCCATCA CCTTCGTCTT CCACCAGCGT 
TCTCATGAGG AGCAGCGGGG CCTCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD54L ( XM_011542300.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540602.1 Homo sapiens RAD54-like (S. cerevisiae) (RAD54L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu61771
Clone ID Related Accession (Same CDS sequence)	XM_011542299.2 , XM_011542300.3 , XM_011542300.2
Accession Version	XM_011542300.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA repair and recombination protein RAD54-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGC GTGGAGCCAG GGTGTCTTCT CCCATCCTCA TCATTTCCTA TGAGACCTTC 
CGCCTTCATG TTGGAGTCCT CCAGAAAGGA AGTGTTGGTC TGGTCATATG TGACGAGGGA 
CACAGGCTCA AGAACTCTGA GAATCAGACT TACCAAGCCC TGGACAGCTT GAACACCAGC 
CGGCGGGTGC TCATCTCCGG AACTCCCATC CAGAATGATC TGCTTGAGTA TTTCAGCTTG 
GTACATTTTG TTAATTCCGG CATCCTAGGG ACTGCCCATG AATTCAAGAA GCATTTTGAA 
TTGCCAATTT TGAAGGGTCG AGACGCTGCT GCTAGTGAGG CAGACAGGCA GCTAGGAGAG 
GAGCGGCTGC GGGAGCTCAC CAGCATTGTG AATAGATGCC TGATACGGAG GACTTCTGAT 
ATCCTTTCTA AATATCTGCC TGTGAAGATT GAGCAGGTCG TTTGTTGTAG GCTGACACCC 
CTTCAGACTG AGTTATACAA GAGGTTTCTG AGACAAGCCA AACCGGCAGA AGAATTGCTT 
GAGGGCAAGA TGAGTGTGTC TTCCCTTTCT TCCATCACCT CGCTAAAGAA GCTTTGTAAT 
CATCCAGCTC TAATCTATGA TAAGTGTGTG GAAGAGGAGG ATGGCTTTGT GGGTGCCTTG 
GACCTCTTCC CTCCTGGTTA CAGCTCTAAG GCCCTGGAGC CCCAGCTGTC AGGTAAGATG 
CTGGTCCTGG ATTATATTCT GGCGGTGACC CGAAGCCGTA GCAGTGACAA AGTAGTGCTG 
GTGTCGAATT ACACCCAGAC TTTGGATCTC TTTGAGAAGC TGTGCCGTGC CCGAAGGTAC 
TTATACGTCC GCCTGGATGG CACGATGTCC ATTAAGAAGC GAGCCAAGGT TGTAGAACGC 
TTCAATAGTC CATCGAGCCC TGACTTTGTC TTCATGCTGA GCAGCAAAGC TGGGGGCTGT 
GGCCTCAATC TCATTGGGGC TAACCGGCTG GTCATGTTTG ACCCTGACTG GAACCCAGCC 
AATGATGAAC AAGCCATGGC CCGGGTCTGG CGAGATGGTC AAAAGAAGAC TTGCTATATC 
TACCGCCTGC TGTCTGCAGG GACCATTGAG GAGAAGATCT TCCAGCGTCA GAGCCACAAG 
AAGGCACTGA GCAGCTGTGT GGTGGATGAG GAGCAGGATG TAGAGCGCCA CTTCTCTCTG 
GGCGAGTTGA AGGAGCTGTT TATCCTGGAT GAAGCTAGCC TCAGTGACAC ACATGACAGG 
TTGCACTGCC GACGTTGTGT CAACAGCCGT CAGATCCGGC CACCCCCTGA TGGTTCTGAC 
TGCACTTCAG ACCTGGCAGG GTGGAACCAC TGCACTGATA AGTGGGGGCT CCGGGATGAG 
GTACTCCAGG CTGCCTGGGA TGCTGCCTCC ACTGCCATCA CCTTCGTCTT CCACCAGCGT 
TCTCATGAGG AGCAGCGGGG CCTCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540602.1
CDS	72..1541
Translation	MNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Target ORF information:

RefSeq Version	XM_011542300.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD54-like (S. cerevisiae) (RAD54L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542300.2

ORF Insert Sequence:

ATGAACCAGC GTGGAGCCAG GGTGTCTTCT CCCATCCTCA TCATTTCCTA TGAGACCTTC 
CGCCTTCATG TTGGAGTCCT CCAGAAAGGA AGTGTTGGTC TGGTCATATG TGACGAGGGA 
CACAGGCTCA AGAACTCTGA GAATCAGACT TACCAAGCCC TGGACAGCTT GAACACCAGC 
CGGCGGGTGC TCATCTCCGG AACTCCCATC CAGAATGATC TGCTTGAGTA TTTCAGCTTG 
GTACATTTTG TTAATTCCGG CATCCTAGGG ACTGCCCATG AATTCAAGAA GCATTTTGAA 
TTGCCAATTT TGAAGGGTCG AGACGCTGCT GCTAGTGAGG CAGACAGGCA GCTAGGAGAG 
GAGCGGCTGC GGGAGCTCAC CAGCATTGTG AATAGATGCC TGATACGGAG GACTTCTGAT 
ATCCTTTCTA AATATCTGCC TGTGAAGATT GAGCAGGTCG TTTGTTGTAG GCTGACACCC 
CTTCAGACTG AGTTATACAA GAGGTTTCTG AGACAAGCCA AACCGGCAGA AGAATTGCTT 
GAGGGCAAGA TGAGTGTGTC TTCCCTTTCT TCCATCACCT CGCTAAAGAA GCTTTGTAAT 
CATCCAGCTC TAATCTATGA TAAGTGTGTG GAAGAGGAGG ATGGCTTTGT GGGTGCCTTG 
GACCTCTTCC CTCCTGGTTA CAGCTCTAAG GCCCTGGAGC CCCAGCTGTC AGGTAAGATG 
CTGGTCCTGG ATTATATTCT GGCGGTGACC CGAAGCCGTA GCAGTGACAA AGTAGTGCTG 
GTGTCGAATT ACACCCAGAC TTTGGATCTC TTTGAGAAGC TGTGCCGTGC CCGAAGGTAC 
TTATACGTCC GCCTGGATGG CACGATGTCC ATTAAGAAGC GAGCCAAGGT TGTAGAACGC 
TTCAATAGTC CATCGAGCCC TGACTTTGTC TTCATGCTGA GCAGCAAAGC TGGGGGCTGT 
GGCCTCAATC TCATTGGGGC TAACCGGCTG GTCATGTTTG ACCCTGACTG GAACCCAGCC 
AATGATGAAC AAGCCATGGC CCGGGTCTGG CGAGATGGTC AAAAGAAGAC TTGCTATATC 
TACCGCCTGC TGTCTGCAGG GACCATTGAG GAGAAGATCT TCCAGCGTCA GAGCCACAAG 
AAGGCACTGA GCAGCTGTGT GGTGGATGAG GAGCAGGATG TAGAGCGCCA CTTCTCTCTG 
GGCGAGTTGA AGGAGCTGTT TATCCTGGAT GAAGCTAGCC TCAGTGACAC ACATGACAGG 
TTGCACTGCC GACGTTGTGT CAACAGCCGT CAGATCCGGC CACCCCCTGA TGGTTCTGAC 
TGCACTTCAG ACCTGGCAGG GTGGAACCAC TGCACTGATA AGTGGGGGCT CCGGGATGAG 
GTACTCCAGG CTGCCTGGGA TGCTGCCTCC ACTGCCATCA CCTTCGTCTT CCACCAGCGT 
TCTCATGAGG AGCAGCGGGG CCTCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RAD54 N-terminal domain is a DNA sensor that couples ATP hydrolysis with branch migration of Holliday junctions.
Nature communications9(1)34(2018 01)
Goyal N,Rossi MJ,Mazina OM,Chi Y,Moritz RL,Clurman BE,Mazin AV

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Cross-Species Genomics Identifies TAF12, NFYC, and RAD54L as Choroid Plexus Carcinoma Oncogenes.
Cancer cell27(5)712-27(2015 May)
Tong Y,Merino D,Nimmervoll B,Gupta K,Wang YD,Finkelstein D,Dalton J,Ellison DW,Ma X,Zhang J,Malkin D,Gilbertson RJ

RAD54 family translocases counter genotoxic effects of RAD51 in human tumor cells.
Nucleic acids research43(6)3180-96(2015 Mar)
Mason JM,Dusad K,Wright WD,Grubb J,Budke B,Heyer WD,Connell PP,Weichselbaum RR,Bishop DK

Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1.
Scientific reports44863(2014 May)
Machida S,Takaku M,Ikura M,Sun J,Suzuki H,Kobayashi W,Kinomura A,Osakabe A,Tachiwana H,Horikoshi Y,Fukuto A,Matsuda R,Ura K,Tashiro S,Ikura T,Kurumizaka H

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RAD54L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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