CCDC77 cDNA ORF clone, Homo sapiens(human)

The following CCDC77 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCDC77 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu31991 NM_032358.4
Latest version!
Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu07022 NM_001130146.2
Latest version!
Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu07022 NM_001130148.2
Latest version!
Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu07022 NM_001130147.1
Latest version!
Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
View Old Accession Versions >>
OHu07022 NM_001130146.1 Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu31991 NM_032358.3 Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu07022 NM_001130148.1 Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu31991
    Clone ID Related Accession (Same CDS sequence) NM_032358.4 , NM_032358.3
    Accession Version NM_032358.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product coiled-coil domain-containing protein 77 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA711064.1, BC006444.2 and AC005844.8. On Nov 22, 2018 this sequence version replaced NM_032358.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK027638.1, SRR1660807.18891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000239830.9/ ENSP00000239830.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAACTTTA CCCCAACACA CACCCCTGTC TGCAGAAAGC GAACAGTTGT CTCCAAACGT 
    GGTGTTGCCG TCAGTGGTCC CACCAAGAGG AGGGGAATGG CAGATTCACT GGAGTCAACC
    CCCTTGCCTT CCCCCGAAGA TCGTCTGGCC AAACTCCATC CTTCTAAGGA GCTCCTGGAA
    TATTATCAAA AGAAGATGGC TGAGTGTGAG GCAGAAAATG AGGACTTGCT GAAGAAACTG
    GAACTCTACA AAGAAGCTTG TGAAGGACAG CATAAACTTG AATGTGATTT GCAGCAGAGG
    GAGGAAGAGA TTGCTGAATT GCAGAAAGCT CTAAGTGATA TGCAGGTCTG CCTCTTCCAG
    GAACGGGAAC ATGTTTTACG CCTCTACTCA GAAAATGACC GACTGAGAAT CAGGGAGCTA
    GAAGACAAGA AAAAGATTCA GAATCTCTTG GCTCTTGTGG GAACAGATGC TGGAGAAGTG
    ACCTATTTTT GTAAGGAGCC TCCTCACAAA GTCACCATTC TCCAAAAGAC TATCCAGGCT
    GTAGGTGAAT GTGAGCAGAG TGAATCTTCA GCTTTCAAAG CAGATCCTAA AATAAGCAAA
    AGAAGACCAT CGAGAGAGAG AAAAGAAAGT TCTGAGCATT ACCAAAGAGA CATACAGACA
    CTCATCCTAC AGGTGGAAGC ACTGCAGGCT CAGCTGGGAG AGCAGACCAA ACTTTCTCGA
    GAACAAATTG AAGGGCTCAT CGAGGACAGA CGGATTCACC TTGAGGAAAT ACAAGTTCAG
    CACCAGAGAA ATCAGAACAA AATCAAAGAG CTAACCAAAA ATCTTCACCA CACCCAAGAA
    CTGCTCTATG AGAGCACCAA AGATTTTCTG CAACTCAGAT CTGAAAACCA AAATAAAGAG
    AAGTCATGGA TGCTTGAAAA AGATAATTTG ATGTCAAAGA TTAAGCAATA TAGGGTGCAG
    TGTAAGAAGA AAGAAGATAA AATTGGAAAA GTGTTGCCCG TTATGCATGA GAGTCACCAT
    GCTCAAAGTG AATATATTAA GTCCCTAAAA GATAAGTTAG TACAAGAGAA AAAGCTGTCC
    AATATGTACC AAGAGCAGTG CATTTCCTTA GAAGAAGAAC TTGCCCGAAT TCGTGAGGAA
    GAGGGAATGA GGAGAGAGAT CTTCAAGGAT CGCACTAACA AGATGGGGAA GCGTTTACAG
    ATAATGACAA AACGCTATGA GGCATTGGAG CGTCGACGTA TCCTGGAAGT AGAAGGCTTT
    AAGACAGATA TTAAAGTTCT CCGACAGAAA CTGAAAGACT TGGAGCAAAT GTTGTATAAG
    GCAACAGTTA ATGCCCGGGC AAACCAGGAT CTTGCCCTTC TGTGTGAGGT CCGTGACAGC
    AATAGACGGG CACATAAGAT ACAAGGAGAA CTGAAGAATC TTAAGTCGAA AGTGTTTGGT
    CTGGAGAATG AACTTAGACT CTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_115734.1
    CDS112..1578
    Translation

    Target ORF information:

    RefSeq Version NM_032358.4
    Organism Homo sapiens(human)
    Definition Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_032358.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAACTTTA CCCCAACACA CACCCCTGTC TGCAGAAAGC GAACAGTTGT CTCCAAACGT 
    GGTGTTGCCG TCAGTGGTCC CACCAAGAGG AGGGGAATGG CAGATTCACT GGAGTCAACC
    CCCTTGCCTT CCCCCGAAGA TCGTCTGGCC AAACTCCATC CTTCTAAGGA GCTCCTGGAA
    TATTATCAAA AGAAGATGGC TGAGTGTGAG GCAGAAAATG AGGACTTGCT GAAGAAACTG
    GAACTCTACA AAGAAGCTTG TGAAGGACAG CATAAACTTG AATGTGATTT GCAGCAGAGG
    GAGGAAGAGA TTGCTGAATT GCAGAAAGCT CTAAGTGATA TGCAGGTCTG CCTCTTCCAG
    GAACGGGAAC ATGTTTTACG CCTCTACTCA GAAAATGACC GACTGAGAAT CAGGGAGCTA
    GAAGACAAGA AAAAGATTCA GAATCTCTTG GCTCTTGTGG GAACAGATGC TGGAGAAGTG
    ACCTATTTTT GTAAGGAGCC TCCTCACAAA GTCACCATTC TCCAAAAGAC TATCCAGGCT
    GTAGGTGAAT GTGAGCAGAG TGAATCTTCA GCTTTCAAAG CAGATCCTAA AATAAGCAAA
    AGAAGACCAT CGAGAGAGAG AAAAGAAAGT TCTGAGCATT ACCAAAGAGA CATACAGACA
    CTCATCCTAC AGGTGGAAGC ACTGCAGGCT CAGCTGGGAG AGCAGACCAA ACTTTCTCGA
    GAACAAATTG AAGGGCTCAT CGAGGACAGA CGGATTCACC TTGAGGAAAT ACAAGTTCAG
    CACCAGAGAA ATCAGAACAA AATCAAAGAG CTAACCAAAA ATCTTCACCA CACCCAAGAA
    CTGCTCTATG AGAGCACCAA AGATTTTCTG CAACTCAGAT CTGAAAACCA AAATAAAGAG
    AAGTCATGGA TGCTTGAAAA AGATAATTTG ATGTCAAAGA TTAAGCAATA TAGGGTGCAG
    TGTAAGAAGA AAGAAGATAA AATTGGAAAA GTGTTGCCCG TTATGCATGA GAGTCACCAT
    GCTCAAAGTG AATATATTAA GTCCCTAAAA GATAAGTTAG TACAAGAGAA AAAGCTGTCC
    AATATGTACC AAGAGCAGTG CATTTCCTTA GAAGAAGAAC TTGCCCGAAT TCGTGAGGAA
    GAGGGAATGA GGAGAGAGAT CTTCAAGGAT CGCACTAACA AGATGGGGAA GCGTTTACAG
    ATAATGACAA AACGCTATGA GGCATTGGAG CGTCGACGTA TCCTGGAAGT AGAAGGCTTT
    AAGACAGATA TTAAAGTTCT CCGACAGAAA CTGAAAGACT TGGAGCAAAT GTTGTATAAG
    GCAACAGTTA ATGCCCGGGC AAACCAGGAT CTTGCCCTTC TGTGTGAGGT CCGTGACAGC
    AATAGACGGG CACATAAGAT ACAAGGAGAA CTGAAGAATC TTAAGTCGAA AGTGTTTGGT
    CTGGAGAATG AACTTAGACT CTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31991
    Clone ID Related Accession (Same CDS sequence) NM_032358.4 , NM_032358.3
    Accession Version NM_032358.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product coiled-coil domain-containing protein 77 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA711064.1, BC006444.2 and AC005844.8. On Jul 18, 2008 this sequence version replaced NM_032358.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1803615.59242.1, SRR1660807.18891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAACTTTA CCCCAACACA CACCCCTGTC TGCAGAAAGC GAACAGTTGT CTCCAAACGT 
    GGTGTTGCCG TCAGTGGTCC CACCAAGAGG AGGGGAATGG CAGATTCACT GGAGTCAACC
    CCCTTGCCTT CCCCCGAAGA TCGTCTGGCC AAACTCCATC CTTCTAAGGA GCTCCTGGAA
    TATTATCAAA AGAAGATGGC TGAGTGTGAG GCAGAAAATG AGGACTTGCT GAAGAAACTG
    GAACTCTACA AAGAAGCTTG TGAAGGACAG CATAAACTTG AATGTGATTT GCAGCAGAGG
    GAGGAAGAGA TTGCTGAATT GCAGAAAGCT CTAAGTGATA TGCAGGTCTG CCTCTTCCAG
    GAACGGGAAC ATGTTTTACG CCTCTACTCA GAAAATGACC GACTGAGAAT CAGGGAGCTA
    GAAGACAAGA AAAAGATTCA GAATCTCTTG GCTCTTGTGG GAACAGATGC TGGAGAAGTG
    ACCTATTTTT GTAAGGAGCC TCCTCACAAA GTCACCATTC TCCAAAAGAC TATCCAGGCT
    GTAGGTGAAT GTGAGCAGAG TGAATCTTCA GCTTTCAAAG CAGATCCTAA AATAAGCAAA
    AGAAGACCAT CGAGAGAGAG AAAAGAAAGT TCTGAGCATT ACCAAAGAGA CATACAGACA
    CTCATCCTAC AGGTGGAAGC ACTGCAGGCT CAGCTGGGAG AGCAGACCAA ACTTTCTCGA
    GAACAAATTG AAGGGCTCAT CGAGGACAGA CGGATTCACC TTGAGGAAAT ACAAGTTCAG
    CACCAGAGAA ATCAGAACAA AATCAAAGAG CTAACCAAAA ATCTTCACCA CACCCAAGAA
    CTGCTCTATG AGAGCACCAA AGATTTTCTG CAACTCAGAT CTGAAAACCA AAATAAAGAG
    AAGTCATGGA TGCTTGAAAA AGATAATTTG ATGTCAAAGA TTAAGCAATA TAGGGTGCAG
    TGTAAGAAGA AAGAAGATAA AATTGGAAAA GTGTTGCCCG TTATGCATGA GAGTCACCAT
    GCTCAAAGTG AATATATTAA GTCCCTAAAA GATAAGTTAG TACAAGAGAA AAAGCTGTCC
    AATATGTACC AAGAGCAGTG CATTTCCTTA GAAGAAGAAC TTGCCCGAAT TCGTGAGGAA
    GAGGGAATGA GGAGAGAGAT CTTCAAGGAT CGCACTAACA AGATGGGGAA GCGTTTACAG
    ATAATGACAA AACGCTATGA GGCATTGGAG CGTCGACGTA TCCTGGAAGT AGAAGGCTTT
    AAGACAGATA TTAAAGTTCT CCGACAGAAA CTGAAAGACT TGGAGCAAAT GTTGTATAAG
    GCAACAGTTA ATGCCCGGGC AAACCAGGAT CTTGCCCTTC TGTGTGAGGT CCGTGACAGC
    AATAGACGGG CACATAAGAT ACAAGGAGAA CTGAAGAATC TTAAGTCGAA AGTGTTTGGT
    CTGGAGAATG AACTTAGACT CTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_115734.1
    CDS181..1647
    Translation

    Target ORF information:

    RefSeq Version NM_032358.3
    Organism Homo sapiens(human)
    Definition Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_032358.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAACTTTA CCCCAACACA CACCCCTGTC TGCAGAAAGC GAACAGTTGT CTCCAAACGT 
    GGTGTTGCCG TCAGTGGTCC CACCAAGAGG AGGGGAATGG CAGATTCACT GGAGTCAACC
    CCCTTGCCTT CCCCCGAAGA TCGTCTGGCC AAACTCCATC CTTCTAAGGA GCTCCTGGAA
    TATTATCAAA AGAAGATGGC TGAGTGTGAG GCAGAAAATG AGGACTTGCT GAAGAAACTG
    GAACTCTACA AAGAAGCTTG TGAAGGACAG CATAAACTTG AATGTGATTT GCAGCAGAGG
    GAGGAAGAGA TTGCTGAATT GCAGAAAGCT CTAAGTGATA TGCAGGTCTG CCTCTTCCAG
    GAACGGGAAC ATGTTTTACG CCTCTACTCA GAAAATGACC GACTGAGAAT CAGGGAGCTA
    GAAGACAAGA AAAAGATTCA GAATCTCTTG GCTCTTGTGG GAACAGATGC TGGAGAAGTG
    ACCTATTTTT GTAAGGAGCC TCCTCACAAA GTCACCATTC TCCAAAAGAC TATCCAGGCT
    GTAGGTGAAT GTGAGCAGAG TGAATCTTCA GCTTTCAAAG CAGATCCTAA AATAAGCAAA
    AGAAGACCAT CGAGAGAGAG AAAAGAAAGT TCTGAGCATT ACCAAAGAGA CATACAGACA
    CTCATCCTAC AGGTGGAAGC ACTGCAGGCT CAGCTGGGAG AGCAGACCAA ACTTTCTCGA
    GAACAAATTG AAGGGCTCAT CGAGGACAGA CGGATTCACC TTGAGGAAAT ACAAGTTCAG
    CACCAGAGAA ATCAGAACAA AATCAAAGAG CTAACCAAAA ATCTTCACCA CACCCAAGAA
    CTGCTCTATG AGAGCACCAA AGATTTTCTG CAACTCAGAT CTGAAAACCA AAATAAAGAG
    AAGTCATGGA TGCTTGAAAA AGATAATTTG ATGTCAAAGA TTAAGCAATA TAGGGTGCAG
    TGTAAGAAGA AAGAAGATAA AATTGGAAAA GTGTTGCCCG TTATGCATGA GAGTCACCAT
    GCTCAAAGTG AATATATTAA GTCCCTAAAA GATAAGTTAG TACAAGAGAA AAAGCTGTCC
    AATATGTACC AAGAGCAGTG CATTTCCTTA GAAGAAGAAC TTGCCCGAAT TCGTGAGGAA
    GAGGGAATGA GGAGAGAGAT CTTCAAGGAT CGCACTAACA AGATGGGGAA GCGTTTACAG
    ATAATGACAA AACGCTATGA GGCATTGGAG CGTCGACGTA TCCTGGAAGT AGAAGGCTTT
    AAGACAGATA TTAAAGTTCT CCGACAGAAA CTGAAAGACT TGGAGCAAAT GTTGTATAAG
    GCAACAGTTA ATGCCCGGGC AAACCAGGAT CTTGCCCTTC TGTGTGAGGT CCGTGACAGC
    AATAGACGGG CACATAAGAT ACAAGGAGAA CTGAAGAATC TTAAGTCGAA AGTGTTTGGT
    CTGGAGAATG AACTTAGACT CTGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07022
    Clone ID Related Accession (Same CDS sequence) NM_001130147.1 , NM_001130148.1 , NM_001130146.1 , NM_001130146.2 , NM_001130148.2
    Accession Version NM_001130147.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product coiled-coil domain-containing protein 77 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC331633.1, AK308216.1, BC006444.2 and AC005844.8. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.38879.1, SRR1803611.139446.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123619.1
    CDS250..1620
    Translation

    Target ORF information:

    RefSeq Version NM_001130147.1
    Organism Homo sapiens(human)
    Definition Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07022
    Clone ID Related Accession (Same CDS sequence) NM_001130147.1 , NM_001130148.1 , NM_001130146.1 , NM_001130146.2 , NM_001130148.2
    Accession Version NM_001130148.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product coiled-coil domain-containing protein 77 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC331633.1, BC006444.2 and AC005844.8. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.151154.1, SRR1803617.156706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123620.1
    CDS196..1566
    Translation

    Target ORF information:

    RefSeq Version NM_001130148.1
    Organism Homo sapiens(human)
    Definition Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07022
    Clone ID Related Accession (Same CDS sequence) NM_001130147.1 , NM_001130148.1 , NM_001130146.1 , NM_001130146.2 , NM_001130148.2
    Accession Version NM_001130146.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product coiled-coil domain-containing protein 77 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA711064.1, CX784327.1, BC006444.2 and AC005844.8. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.118203.1, SRR1803616.129396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123618.1
    CDS223..1593
    Translation

    Target ORF information:

    RefSeq Version NM_001130146.1
    Organism Homo sapiens(human)
    Definition Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07022
    Clone ID Related Accession (Same CDS sequence) NM_001130147.1 , NM_001130148.1 , NM_001130146.1 , NM_001130146.2 , NM_001130148.2
    Accession Version NM_001130146.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product coiled-coil domain-containing protein 77 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA711064.1, CX784327.1, BC006444.2 and AC005844.8. On May 31, 2019 this sequence version replaced NM_001130146.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1003452.1, SRR1660807.93781.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123618.1
    CDS154..1524
    Translation

    Target ORF information:

    RefSeq Version NM_001130146.2
    Organism Homo sapiens(human)
    Definition Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130146.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07022
    Clone ID Related Accession (Same CDS sequence) NM_001130147.1 , NM_001130148.1 , NM_001130146.1 , NM_001130146.2 , NM_001130148.2
    Accession Version NM_001130148.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product coiled-coil domain-containing protein 77 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC005844.8, DC331633.1 and BC006444.2. On May 31, 2019 this sequence version replaced NM_001130148.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.151154.1, SRR1803617.156706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123620.1
    CDS199..1569
    Translation

    Target ORF information:

    RefSeq Version NM_001130148.2
    Organism Homo sapiens(human)
    Definition Homo sapiens coiled-coil domain containing 77 (CCDC77), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130148.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAGATT CACTGGAGTC AACCCCCTTG CCTTCCCCCG AAGATCGTCT GGCCAAACTC 
    CATCCTTCTA AGGAGCTCCT GGAATATTAT CAAAAGAAGA TGGCTGAGTG TGAGGCAGAA
    AATGAGGACT TGCTGAAGAA ACTGGAACTC TACAAAGAAG CTTGTGAAGG ACAGCATAAA
    CTTGAATGTG ATTTGCAGCA GAGGGAGGAA GAGATTGCTG AATTGCAGAA AGCTCTAAGT
    GATATGCAGG TCTGCCTCTT CCAGGAACGG GAACATGTTT TACGCCTCTA CTCAGAAAAT
    GACCGACTGA GAATCAGGGA GCTAGAAGAC AAGAAAAAGA TTCAGAATCT CTTGGCTCTT
    GTGGGAACAG ATGCTGGAGA AGTGACCTAT TTTTGTAAGG AGCCTCCTCA CAAAGTCACC
    ATTCTCCAAA AGACTATCCA GGCTGTAGGT GAATGTGAGC AGAGTGAATC TTCAGCTTTC
    AAAGCAGATC CTAAAATAAG CAAAAGAAGA CCATCGAGAG AGAGAAAAGA AAGTTCTGAG
    CATTACCAAA GAGACATACA GACACTCATC CTACAGGTGG AAGCACTGCA GGCTCAGCTG
    GGAGAGCAGA CCAAACTTTC TCGAGAACAA ATTGAAGGGC TCATCGAGGA CAGACGGATT
    CACCTTGAGG AAATACAAGT TCAGCACCAG AGAAATCAGA ACAAAATCAA AGAGCTAACC
    AAAAATCTTC ACCACACCCA AGAACTGCTC TATGAGAGCA CCAAAGATTT TCTGCAACTC
    AGATCTGAAA ACCAAAATAA AGAGAAGTCA TGGATGCTTG AAAAAGATAA TTTGATGTCA
    AAGATTAAGC AATATAGGGT GCAGTGTAAG AAGAAAGAAG ATAAAATTGG AAAAGTGTTG
    CCCGTTATGC ATGAGAGTCA CCATGCTCAA AGTGAATATA TTAAGTCCCT AAAAGATAAG
    TTAGTACAAG AGAAAAAGCT GTCCAATATG TACCAAGAGC AGTGCATTTC CTTAGAAGAA
    GAACTTGCCC GAATTCGTGA GGAAGAGGGA ATGAGGAGAG AGATCTTCAA GGATCGCACT
    AACAAGATGG GGAAGCGTTT ACAGATAATG ACAAAACGCT ATGAGGCATT GGAGCGTCGA
    CGTATCCTGG AAGTAGAAGG CTTTAAGACA GATATTAAAG TTCTCCGACA GAAACTGAAA
    GACTTGGAGC AAATGTTGTA TAAGGCAACA GTTAATGCCC GGGCAAACCA GGATCTTGCC
    CTTCTGTGTG AGGTCCGTGA CAGCAATAGA CGGGCACATA AGATACAAGG AGAACTGAAG
    AATCTTAAGT CGAAAGTGTT TGGTCTGGAG AATGAACTTA GACTCTGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.