NOA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOA1
Entrez Gene ID	84273
Full Name	nitric oxide associated 1
Synonyms	C4orf14,MTG3,hAtNOS1,hNOA1,mAtNOS1
General protein information	Preferred Names nitric oxide associated 1 Names nitric oxide-associated protein 1 mitochondrial GTPase 3 homolog nitric oxide synthase, mitochondrial (putative) putative ortholog of Arabidopsis mitochondrial nitric oxide synthase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q12
Summary	The protein encoded by this gene is a nuclear-encoded GTPase that functions in the mitochondrion. Upon translation, this protein is imported into the nucleus and then into the nucleolus before being exported to the mitochondrion. The encoded protein is required for oxygen-dependent regulation of mitochondrial respiratory complexes and for mitochondrial protein synthesis. [provided by RefSeq, Dec 2015].
Disorder MIM:	614919

mRNA and Protein(s)

mRNA	Protein	Name
NM_032313.3	NP_115689.1	nitric oxide-associated protein 1
NM_032313.2	NP_115689.1	nitric oxide-associated protein 1
NM_032313.4	NP_115689.1	nitric oxide-associated protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NOA1	XP_854207.1
Bos taurus (cattle)	NOA1	NP_001033277.1
Mus musculus (house mouse)	Noa1	NP_062810.1
Danio rerio (zebrafish)	LOC101883563	XP_005174712.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010271	XP_319462.4
Caenorhabditis elegans (roundworm)	Y48E1B.2	NP_496845.1
Danio rerio (zebrafish)	noa1	NP_001107900.1
Homo sapiens (human)	NOA1	NP_115689.1
Gallus gallus (chicken)	NOA1	XP_420579.4
Drosophila melanogaster (fruit fly)	CG10914	NP_611297.3
Xenopus tropicalis (tropical clawed frog)	noa1	XP_002937599.2
Pan troglodytes (chimpanzee)	NOA1	XP_001136575.2
	C4orf14	XP_001082521.1
Rattus norvegicus (Norway rat)	Noa1	NP_001006960.1

Gene Ontology
Bibliography

Related articles in PubMed

NOA1, a novel ClpXP substrate, takes an unexpected nuclear detour prior to mitochondrial import.
Al-Furoukh N, Kardon JR, Kr?ger M, Szibor M, Baker TA, Braun T
PloS one9(7)e103141(2014)

Human C4orf14 interacts with the mitochondrial nucleoid and is involved in the biogenesis of the small mitochondrial ribosomal subunit.
He J, Cooper HM, Reyes A, Di Re M, Kazak L, Wood SR, Mao CC, Fearnley IM, Walker JE, Holt IJ
Nucleic acids research40(13)6097-108(2012 Jul)

Nitric oxide-associated protein 1 (NOA1) is necessary for oxygen-dependent regulation of mitochondrial respiratory complexes.
Heidler J, Al-Furoukh N, Kukat C, Salwig I, Ingelmann ME, Seibel P, Kr?ger M, Holtz J, Wittig I, Braun T, Szibor M
The Journal of biological chemistry286(37)32086-93(2011 Sep)

Genome-wide association study identifies two susceptibility loci for exudative age-related macular degeneration in the Japanese population.
Arakawa S, Takahashi A, Ashikawa K, Hosono N, Aoi T, Yasuda M, Oshima Y, Yoshida S, Enaida H, Tsuchihashi T, Mori K, Honda S, Negi A, Arakawa A, Kadonosono K, Kiyohara Y, Kamatani N, Nakamura Y, Ishibashi T, Kubo M
Nature genetics43(10)1001-4(2011 Sep)

NOA1 is an essential GTPase required for mitochondrial protein synthesis.
Kolanczyk M, Pech M, Zemojtel T, Yamamoto H, Mikula I, Calvaruso MA, van den Brand M, Richter R, Fischer B, Ritz A, Kossler N, Thurisch B, Spoerle R, Smeitink J, Kornak U, Chan D, Vingron M, Martasek P, Lightowlers RN, Nijtmans L, Schuelke M, Nierhaus KH, Mundlos S
Molecular biology of the cell22(1)1-11(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results uncovered an unexpected, mandatory detour of NOA1 through the nucleolus before uptake into mitochondria.
Title: NOA1, a novel ClpXP substrate, takes an unexpected nuclear detour prior to mitochondrial import.

This study indicates rhat C4orf14 is involved in 28S subunit biogenesis and mtDNA maintenance.
Title: Human C4orf14 interacts with the mitochondrial nucleoid and is involved in the biogenesis of the small mitochondrial ribosomal subunit.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The present study suggests a novel function for mAtNOS1 in regulating mitochondrial nitric oxide and calcium and inducing apoptosis of MCF-7 cells
Title: mAtNOS1 induces apoptosis of human mammary adenocarcinoma cells.

The following NOA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04962	NM_032313.2 Latest version!	Homo sapiens nitric oxide associated 1 (NOA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu101465	NM_032313.4 Latest version!	Homo sapiens nitric oxide associated 1 (NOA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu101465	NM_032313.3	Homo sapiens nitric oxide associated 1 (NOA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOA1 ( NM_032313.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_115689.1 Homo sapiens nitric oxide associated 1 (NOA1), mRNA.

Price & Availability↑

CloneID	OHu04962
Clone ID Related Accession (Same CDS sequence)	NM_032313.2
Accession Version	NM_032313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	nitric oxide-associated protein 1
Comment

ATGCTGCCCG CTCGCCTACC GTTCAGGCTG CTGAGCCTTT TCCTTCGTGG ATCCGCTCCC 
ACGGCAGCGC GCCATGGCCT CCGGGAGCCG CTCCTGGAGA GGAGGTGCGC TGCCGCCTCC 
TCCTTCCAGC ACTCATCGAG TCTGGGACGC GAGCTTCCTT ATGACCCCGT GGACACGGAG 
GGCTTTGGAG AAGGTGGTGA CATGCAGGAG CGTTTTCTGT TCCCGGAGTA CATCCTGGAT 
CCGGAGCCGC AACCCACCCG CGAAAAGCAG CTGCAGGAGC TCCAGCAACA GCAGGAGGAG 
GAGGAGCGAC AGAGGCAGCA GCGGCGGGAG GAGCGGCGAC AGCAAAACCT ACGGGCCAGG 
TCCCGGGAGC ACCCGGTCGT GGGGCACCCG GACCCGGCAT TGCCGCCCAG CGGCGTGAAC 
TGCTCGGGCT GCGGGGCAGA GCTGCACTGC CAGGACGCCG GAGTGCCCGG CTACCTGCCC 
CGAGAGAAGT TCCTCCGCAC GGCGGAGGCA GACGGCGGGC TGGCACGGAC CGTGTGCCAG 
CGCTGCTGGC TGCTGTCGCA CCACCGGCGC GCTCTACGCC TGCAGGTGAG CCGCGAGCAG 
TACCTGGAGC TGGTGAGCGC CGCGTTGCGG CGGCCCGGCC CCTCCCTGGT GCTCTACATG 
GTGGACCTGC TGGACCTGCC CGACGCCCTG CTGCCCGACT TGCCCGCGCT GGTGGGCCCC 
AAGCAGCTGA TCGTGCTGGG AAACAAAGTG GACCTCCTGC CCCAGGATGC TCCTGGCTAC 
CGGCAGAGGC TGCGGGAGCG ACTGTGGGAG GACTGTGCCC GCGCCGGGCT CCTGCTGGCC 
CCTGGCCACC AAGGGCCACA GCGCCCCGTC AAGGACGAGC CACAGGACGG GGAGAATCCG 
AATCCGCCGA ACTGGTCCCG CACAGTGGTC AGGGACGTGC GGCTGATCAG CGCCAAGACC 
GGCTATGGAG TGGAAGAGTT GATCTCTGCC CTTCAGCGCT CCTGGCGCTA CCGTGGGGAC 
GTCTACTTAG TGGGCGCCAC CAACGCCGGC AAATCCACTC TCTTTAACAC GCTCCTGGAG 
TCCGATTACT GCACTGCCAA GGGCTCCGAG GCCATCGACA GAGCCACCAT CTCCCCTTGG 
CCAGGTACTA CATTAAACCT TCTGAAGTTT CCTATTTGCA ACCCAACTCC TTACAGAATG 
TTTAAAAGGC ATCAAAGACT TAAAAAAGAT TCAACTCAAG CTGAAGAAGA TCTTAGTGAG 
CAAGAACAAA ATCAGCTTAA TGTCCTCAAA AAGCATGGTT ATGTCGTAGG AAGAGTTGGA 
AGGACATTCT TGTATTCAGA AGAACAGAAG GATAACATTC CCTTTGAGTT TGATGCTGAT 
TCACTTGCCT TTGACATGGA AAATGACCCT GTTATGGGTA CACACAAATC CACCAAACAA 
GTAGAATTGA CTGCACAAGA TGTGAAAGAT GCCCACTGGT TTTATGACAC CCCTGGAATT 
ACAAAAGAAA ATTGTATTTT AAATCTTCTA ACAGAAAAAG AAGTAAATAT TGTTTTGCCA 
ACACAGTCCA TTGTTCCAAG AACTTTTGTG CTTAAACCAG GAATGGTTCT GTTTTTGGGT 
GCTATAGGCC GCATAGATTT CCTGCAGGGA AATCAGTCAG CTTGGTTTAC AGTCGTGGCT 
TCCAACATCC TCCCTGTGCA TATCACCTCC TTGGACAGGG CAGACGCTCT GTATCAGAAG 
CATGCAGGTC ATACGTTACT CCAGATTCCA ATGGGTGGAA AAGAACGAAT GGCAGGATTT 
CCTCCTCTTG TTGCTGAAGA CATTATGTTA AAAGAAGGAC TGGGGGCATC TGAAGCAGTG 
GCCGACATCA AGTTTTCCTC TGCAGGTTGG GTTTCAGTAA CACCTAATTT TAAGGACAGA 
CTGCATCTCC GAGGCTATAC ACCTGAAGGA ACAGTTTTGA CCGTCCGGCC CCCTCTCTTG 
CCATATATTG TTAACATCAA AGGACAGCGC ATCAAGAAAA GTGTGGCCTA TAAAACCAAG 
AAGCCTCCTT CCCTTATGTA CAACGTGAGG AAGAAGAAAG GAAAGATAAA TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_115689.1

Translation

MLPARLPFRLLSLFLRGSAPTAARHGLREPLLERRCAAASSFQHSSSLGRELPYDPVDTEGFGEGGDMQERFLFPEYILDPEPQPTREKQLQELQQQQEEEERQRQQRREERRQQNLRARSREHPVVGHPDPALPPSGVNCSGCGAELHCQDAGVPGYLPREKFLRTAEADGGLARTVCQRCWLLSHHRRALRLQVSREQYLELVSAALRRPGPSLVLYMVDLLDLPDALLPDLPALVGPKQLIVLGNKVDLLPQDAPGYRQRLRERLWEDCARAGLLLAPGHQGPQRPVKDEPQDGENPNPPNWSRTVVRDVRLISAKTGYGVEELISALQRSWRYRGDVYLVGATNAGKSTLFNTLLESDYCTAKGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMFKRHQRLKKDSTQAEEDLSEQEQNQLNVLKKHGYVVGRVGRTFLYSEEQKDNIPFEFDADSLAFDMENDPVMGTHKSTKQVELTAQDVKDAHWFYDTPGITKENCILNLLTEKEVNIVLPTQSIVPRTFVLKPGMVLFLGAIGRIDFLQGNQSAWFTVVASNILPVHITSLDRADALYQKHAGHTLLQIPMGGKERMAGFPPLVAEDIMLKEGLGASEAVADIKFSSAGWVSVTPNFKDRLHLRGYTPEGTVLTVRPPLLPYIVNIKGQRIKKSVAYKTKKPPSLMYNVRKKKGKINV

Target ORF information:

RefSeq Version	NM_032313.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens nitric oxide associated 1 (NOA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032313.2

ORF Insert Sequence:

ATGCTGCCCG CTCGCCTACC GTTCAGGCTG CTGAGCCTTT TCCTTCGTGG ATCCGCTCCC 
ACGGCAGCGC GCCATGGCCT CCGGGAGCCG CTCCTGGAGA GGAGGTGCGC TGCCGCCTCC 
TCCTTCCAGC ACTCATCGAG TCTGGGACGC GAGCTTCCTT ATGACCCCGT GGACACGGAG 
GGCTTTGGAG AAGGTGGTGA CATGCAGGAG CGTTTTCTGT TCCCGGAGTA CATCCTGGAT 
CCGGAGCCGC AACCCACCCG CGAAAAGCAG CTGCAGGAGC TCCAGCAACA GCAGGAGGAG 
GAGGAGCGAC AGAGGCAGCA GCGGCGGGAG GAGCGGCGAC AGCAAAACCT ACGGGCCAGG 
TCCCGGGAGC ACCCGGTCGT GGGGCACCCG GACCCGGCAT TGCCGCCCAG CGGCGTGAAC 
TGCTCGGGCT GCGGGGCAGA GCTGCACTGC CAGGACGCCG GAGTGCCCGG CTACCTGCCC 
CGAGAGAAGT TCCTCCGCAC GGCGGAGGCA GACGGCGGGC TGGCACGGAC CGTGTGCCAG 
CGCTGCTGGC TGCTGTCGCA CCACCGGCGC GCTCTACGCC TGCAGGTGAG CCGCGAGCAG 
TACCTGGAGC TGGTGAGCGC CGCGTTGCGG CGGCCCGGCC CCTCCCTGGT GCTCTACATG 
GTGGACCTGC TGGACCTGCC CGACGCCCTG CTGCCCGACT TGCCCGCGCT GGTGGGCCCC 
AAGCAGCTGA TCGTGCTGGG AAACAAAGTG GACCTCCTGC CCCAGGATGC TCCTGGCTAC 
CGGCAGAGGC TGCGGGAGCG ACTGTGGGAG GACTGTGCCC GCGCCGGGCT CCTGCTGGCC 
CCTGGCCACC AAGGGCCACA GCGCCCCGTC AAGGACGAGC CACAGGACGG GGAGAATCCG 
AATCCGCCGA ACTGGTCCCG CACAGTGGTC AGGGACGTGC GGCTGATCAG CGCCAAGACC 
GGCTATGGAG TGGAAGAGTT GATCTCTGCC CTTCAGCGCT CCTGGCGCTA CCGTGGGGAC 
GTCTACTTAG TGGGCGCCAC CAACGCCGGC AAATCCACTC TCTTTAACAC GCTCCTGGAG 
TCCGATTACT GCACTGCCAA GGGCTCCGAG GCCATCGACA GAGCCACCAT CTCCCCTTGG 
CCAGGTACTA CATTAAACCT TCTGAAGTTT CCTATTTGCA ACCCAACTCC TTACAGAATG 
TTTAAAAGGC ATCAAAGACT TAAAAAAGAT TCAACTCAAG CTGAAGAAGA TCTTAGTGAG 
CAAGAACAAA ATCAGCTTAA TGTCCTCAAA AAGCATGGTT ATGTCGTAGG AAGAGTTGGA 
AGGACATTCT TGTATTCAGA AGAACAGAAG GATAACATTC CCTTTGAGTT TGATGCTGAT 
TCACTTGCCT TTGACATGGA AAATGACCCT GTTATGGGTA CACACAAATC CACCAAACAA 
GTAGAATTGA CTGCACAAGA TGTGAAAGAT GCCCACTGGT TTTATGACAC CCCTGGAATT 
ACAAAAGAAA ATTGTATTTT AAATCTTCTA ACAGAAAAAG AAGTAAATAT TGTTTTGCCA 
ACACAGTCCA TTGTTCCAAG AACTTTTGTG CTTAAACCAG GAATGGTTCT GTTTTTGGGT 
GCTATAGGCC GCATAGATTT CCTGCAGGGA AATCAGTCAG CTTGGTTTAC AGTCGTGGCT 
TCCAACATCC TCCCTGTGCA TATCACCTCC TTGGACAGGG CAGACGCTCT GTATCAGAAG 
CATGCAGGTC ATACGTTACT CCAGATTCCA ATGGGTGGAA AAGAACGAAT GGCAGGATTT 
CCTCCTCTTG TTGCTGAAGA CATTATGTTA AAAGAAGGAC TGGGGGCATC TGAAGCAGTG 
GCCGACATCA AGTTTTCCTC TGCAGGTTGG GTTTCAGTAA CACCTAATTT TAAGGACAGA 
CTGCATCTCC GAGGCTATAC ACCTGAAGGA ACAGTTTTGA CCGTCCGGCC CCCTCTCTTG 
CCATATATTG TTAACATCAA AGGACAGCGC ATCAAGAAAA GTGTGGCCTA TAAAACCAAG 
AAGCCTCCTT CCCTTATGTA CAACGTGAGG AAGAAGAAAG GAAAGATAAA TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOA1 ( NM_032313.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115689.1 Homo sapiens nitric oxide associated 1 (NOA1), mRNA.

Price & Availability↑

CloneID	OHu101465
Clone ID Related Accession (Same CDS sequence)	NM_032313.3 , NM_032313.4
Accession Version	NM_032313.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	nitric oxide-associated protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC004894.2, AC069307.7 and AA431879.1. On Dec 8, 2015 this sequence version replaced NM_032313.2. ##Evidence-Data-START## Transcript exon combination :: BC004894.2, AK128696.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCCCG CTCGCCTACC GTTCAGGCTG CTGAGCCTTT TCCTTCGTGG ATCCGCTCCC 
ACGGCAGCGC GCCATGGCCT CCGGGAGCCG CTCCTGGAGA GGAGGTGCGC TGCCGCCTCC 
TCCTTCCAGC ACTCATCGAG TCTGGGACGC GAGCTTCCTT ATGACCCCGT GGACACGGAG 
GGCTTTGGAG AAGGTGGTGA CATGCAGGAG CGTTTTCTGT TCCCGGAGTA CATCCTGGAT 
CCGGAGCCGC AACCCACCCG CGAAAAGCAG CTGCAGGAGC TCCAGCAACA GCAGGAGGAG 
GAGGAGCGAC AGAGGCAGCA GCGGCGGGAG GAGCGGCGAC AGCAAAACCT ACGGGCCAGG 
TCCCGGGAGC ACCCGGTCGT GGGGCACCCG GACCCGGCAT TGCCGCCCAG CGGCGTGAAC 
TGCTCGGGCT GTGGGGCAGA GCTGCACTGC CAGGACGCCG GAGTGCCCGG CTACCTGCCC 
CGAGAGAAGT TCCTCCGCAC GGCGGAGGCA GACGGCGGGC TGGCACGGAC CGTGTGCCAG 
CGCTGCTGGC TGCTGTCGCA CCACCGGCGC GCTCTACGCC TGCAGGTGAG CCGCGAGCAG 
TACCTGGAGC TGGTGAGCGC CGCGTTGCGG CGGCCCGGCC CCTCCCTGGT GCTCTACATG 
GTGGACCTGC TGGACCTGCC CGACGCCCTG CTGCCCGACT TGCCCGCGCT GGTGGGCCCC 
AAGCAGCTGA TCGTGCTGGG AAACAAAGTG GACCTCCTGC CCCAGGATGC TCCTGGCTAC 
CGGCAGAGGC TGCGGGAGCG ACTGTGGGAG GACTGTGCCC GCGCCGGGCT CCTGCTGGCC 
CCTGGCCACC AAGGGCCACA GCGCCCCGTC AAGGACGAGC CACAGGACGG GGAGAATCCG 
AATCCGCCGA ACTGGTCCCG CACAGTGGTC AGGGACGTGC GGCTGATCAG CGCCAAGACC 
GGCTATGGAG TGGAAGAGTT GATCTCTGCC CTTCAGCGCT CCTGGCGCTA CCGTGGGGAC 
GTCTACTTAG TGGGCGCCAC CAACGCCGGC AAATCCACTC TCTTTAACAC GCTCCTGGAG 
TCCGATTACT GCACTGCCAA GGGCTCCGAG GCCATCGACA GAGCCACCAT CTCCCCTTGG 
CCAGGTACTA CATTAAACCT TCTGAAGTTT CCTATTTGCA ACCCAACTCC TTACAGAATG 
TTTAAAAGGC ATCAAAGACT TAAAAAAGAT TCAACTCAAG CTGAAGAAGA TCTTAGTGAG 
CAAGAACAAA ATCAGCTTAA TGTCCTCAAA AAGCATGGTT ATGTCGTAGG AAGAGTTGGA 
AGGACATTCT TGTATTCAGA AGAACAGAAG GATAACATTC CCTTTGAGTT TGATGCTGAT 
TCACTTGCCT TTGACATGGA AAATGACCCT GTTATGGGTA CACACAAATC CACCAAACAA 
GTAGAATTGA CTGCACAAGA TGTGAAAGAT GCCCACTGGT TTTATGACAC CCCTGGAATT 
ACAAAAGAAA ATTGTATTTT AAATCTTCTA ACAGAAAAAG AAGTAAATAT TGTTTTGCCA 
ACACAGTCCA TTGTTCCAAG AACTTTTGTG CTTAAACCAG GAATGGTTCT GTTTTTGGGT 
GCTATAGGCC GCATAGATTT CCTGCAGGGA AATCAGTCAG CTTGGTTTAC AGTCGTGGCT 
TCCAACATCC TCCCTGTGCA TATCACCTCC TTGGACAGGG CAGACGCTCT GTATCAGAAG 
CATGCAGGTC ATACGTTACT CCAGATTCCA ATGGGTGGAA AAGAACGAAT GGCAGGATTT 
CCTCCTCTTG TTGCTGAAGA CATTATGTTA AAAGAAGGAC TGGGGGCATC TGAAGCAGTG 
GCCGACATCA AGTTTTCCTC TGCAGGTTGG GTTTCAGTAA CACCTAATTT TAAGGACAGA 
CTGCATCTCC GAGGCTATAC ACCTGAAGGA ACAGTTTTGA CCGTCCGGCC CCCTCTCTTG 
CCATATATTG TTAACATCAA AGGACAGCGC ATCAAGAAAA GTGTGGCCTA TAAAACCAAG 
AAGCCTCCTT CCCTTATGTA CAACGTGAGG AAGAAGAAAG GAAAGATAAA TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115689.1
CDS	76..2172
Translation	MLPARLPFRLLSLFLRGSAPTAARHGLREPLLERRCAAASSFQHSSSLGRELPYDPVDTEGFGEGGDMQERFLFPEYILDPEPQPTREKQLQELQQQQEEEERQRQQRREERRQQNLRARSREHPVVGHPDPALPPSGVNCSGCGAELHCQDAGVPGYLPREKFLRTAEADGGLARTVCQRCWLLSHHRRALRLQVSREQYLELVSAALRRPGPSLVLYMVDLLDLPDALLPDLPALVGPKQLIVLGNKVDLLPQDAPGYRQRLRERLWEDCARAGLLLAPGHQGPQRPVKDEPQDGENPNPPNWSRTVVRDVRLISAKTGYGVEELISALQRSWRYRGDVYLVGATNAGKSTLFNTLLESDYCTAKGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMFKRHQRLKKDSTQAEEDLSEQEQNQLNVLKKHGYVVGRVGRTFLYSEEQKDNIPFEFDADSLAFDMENDPVMGTHKSTKQVELTAQDVKDAHWFYDTPGITKENCILNLLTEKEVNIVLPTQSIVPRTFVLKPGMVLFLGAIGRIDFLQGNQSAWFTVVASNILPVHITSLDRADALYQKHAGHTLLQIPMGGKERMAGFPPLVAEDIMLKEGLGASEAVADIKFSSAGWVSVTPNFKDRLHLRGYTPEGTVLTVRPPLLPYIVNIKGQRIKKSVAYKTKKPPSLMYNVRKKKGKINV

Target ORF information:

RefSeq Version	NM_032313.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide associated 1 (NOA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032313.3

ORF Insert Sequence:

ATGCTGCCCG CTCGCCTACC GTTCAGGCTG CTGAGCCTTT TCCTTCGTGG ATCCGCTCCC 
ACGGCAGCGC GCCATGGCCT CCGGGAGCCG CTCCTGGAGA GGAGGTGCGC TGCCGCCTCC 
TCCTTCCAGC ACTCATCGAG TCTGGGACGC GAGCTTCCTT ATGACCCCGT GGACACGGAG 
GGCTTTGGAG AAGGTGGTGA CATGCAGGAG CGTTTTCTGT TCCCGGAGTA CATCCTGGAT 
CCGGAGCCGC AACCCACCCG CGAAAAGCAG CTGCAGGAGC TCCAGCAACA GCAGGAGGAG 
GAGGAGCGAC AGAGGCAGCA GCGGCGGGAG GAGCGGCGAC AGCAAAACCT ACGGGCCAGG 
TCCCGGGAGC ACCCGGTCGT GGGGCACCCG GACCCGGCAT TGCCGCCCAG CGGCGTGAAC 
TGCTCGGGCT GTGGGGCAGA GCTGCACTGC CAGGACGCCG GAGTGCCCGG CTACCTGCCC 
CGAGAGAAGT TCCTCCGCAC GGCGGAGGCA GACGGCGGGC TGGCACGGAC CGTGTGCCAG 
CGCTGCTGGC TGCTGTCGCA CCACCGGCGC GCTCTACGCC TGCAGGTGAG CCGCGAGCAG 
TACCTGGAGC TGGTGAGCGC CGCGTTGCGG CGGCCCGGCC CCTCCCTGGT GCTCTACATG 
GTGGACCTGC TGGACCTGCC CGACGCCCTG CTGCCCGACT TGCCCGCGCT GGTGGGCCCC 
AAGCAGCTGA TCGTGCTGGG AAACAAAGTG GACCTCCTGC CCCAGGATGC TCCTGGCTAC 
CGGCAGAGGC TGCGGGAGCG ACTGTGGGAG GACTGTGCCC GCGCCGGGCT CCTGCTGGCC 
CCTGGCCACC AAGGGCCACA GCGCCCCGTC AAGGACGAGC CACAGGACGG GGAGAATCCG 
AATCCGCCGA ACTGGTCCCG CACAGTGGTC AGGGACGTGC GGCTGATCAG CGCCAAGACC 
GGCTATGGAG TGGAAGAGTT GATCTCTGCC CTTCAGCGCT CCTGGCGCTA CCGTGGGGAC 
GTCTACTTAG TGGGCGCCAC CAACGCCGGC AAATCCACTC TCTTTAACAC GCTCCTGGAG 
TCCGATTACT GCACTGCCAA GGGCTCCGAG GCCATCGACA GAGCCACCAT CTCCCCTTGG 
CCAGGTACTA CATTAAACCT TCTGAAGTTT CCTATTTGCA ACCCAACTCC TTACAGAATG 
TTTAAAAGGC ATCAAAGACT TAAAAAAGAT TCAACTCAAG CTGAAGAAGA TCTTAGTGAG 
CAAGAACAAA ATCAGCTTAA TGTCCTCAAA AAGCATGGTT ATGTCGTAGG AAGAGTTGGA 
AGGACATTCT TGTATTCAGA AGAACAGAAG GATAACATTC CCTTTGAGTT TGATGCTGAT 
TCACTTGCCT TTGACATGGA AAATGACCCT GTTATGGGTA CACACAAATC CACCAAACAA 
GTAGAATTGA CTGCACAAGA TGTGAAAGAT GCCCACTGGT TTTATGACAC CCCTGGAATT 
ACAAAAGAAA ATTGTATTTT AAATCTTCTA ACAGAAAAAG AAGTAAATAT TGTTTTGCCA 
ACACAGTCCA TTGTTCCAAG AACTTTTGTG CTTAAACCAG GAATGGTTCT GTTTTTGGGT 
GCTATAGGCC GCATAGATTT CCTGCAGGGA AATCAGTCAG CTTGGTTTAC AGTCGTGGCT 
TCCAACATCC TCCCTGTGCA TATCACCTCC TTGGACAGGG CAGACGCTCT GTATCAGAAG 
CATGCAGGTC ATACGTTACT CCAGATTCCA ATGGGTGGAA AAGAACGAAT GGCAGGATTT 
CCTCCTCTTG TTGCTGAAGA CATTATGTTA AAAGAAGGAC TGGGGGCATC TGAAGCAGTG 
GCCGACATCA AGTTTTCCTC TGCAGGTTGG GTTTCAGTAA CACCTAATTT TAAGGACAGA 
CTGCATCTCC GAGGCTATAC ACCTGAAGGA ACAGTTTTGA CCGTCCGGCC CCCTCTCTTG 
CCATATATTG TTAACATCAA AGGACAGCGC ATCAAGAAAA GTGTGGCCTA TAAAACCAAG 
AAGCCTCCTT CCCTTATGTA CAACGTGAGG AAGAAGAAAG GAAAGATAAA TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOA1 ( NM_032313.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115689.1 Homo sapiens nitric oxide associated 1 (NOA1), mRNA.

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CloneID	OHu101465
Clone ID Related Accession (Same CDS sequence)	NM_032313.3 , NM_032313.4
Accession Version	NM_032313.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	nitric oxide-associated protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC004894.2 and AC069307.7. On Nov 22, 2018 this sequence version replaced NM_032313.3. ##Evidence-Data-START## Transcript exon combination :: BC004894.2, SRR3476690.76227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264230.5/ ENSP00000264230.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCCCG CTCGCCTACC GTTCAGGCTG CTGAGCCTTT TCCTTCGTGG ATCCGCTCCC 
ACGGCAGCGC GCCATGGCCT CCGGGAGCCG CTCCTGGAGA GGAGGTGCGC TGCCGCCTCC 
TCCTTCCAGC ACTCATCGAG TCTGGGACGC GAGCTTCCTT ATGACCCCGT GGACACGGAG 
GGCTTTGGAG AAGGTGGTGA CATGCAGGAG CGTTTTCTGT TCCCGGAGTA CATCCTGGAT 
CCGGAGCCGC AACCCACCCG CGAAAAGCAG CTGCAGGAGC TCCAGCAACA GCAGGAGGAG 
GAGGAGCGAC AGAGGCAGCA GCGGCGGGAG GAGCGGCGAC AGCAAAACCT ACGGGCCAGG 
TCCCGGGAGC ACCCGGTCGT GGGGCACCCG GACCCGGCAT TGCCGCCCAG CGGCGTGAAC 
TGCTCGGGCT GTGGGGCAGA GCTGCACTGC CAGGACGCCG GAGTGCCCGG CTACCTGCCC 
CGAGAGAAGT TCCTCCGCAC GGCGGAGGCA GACGGCGGGC TGGCACGGAC CGTGTGCCAG 
CGCTGCTGGC TGCTGTCGCA CCACCGGCGC GCTCTACGCC TGCAGGTGAG CCGCGAGCAG 
TACCTGGAGC TGGTGAGCGC CGCGTTGCGG CGGCCCGGCC CCTCCCTGGT GCTCTACATG 
GTGGACCTGC TGGACCTGCC CGACGCCCTG CTGCCCGACT TGCCCGCGCT GGTGGGCCCC 
AAGCAGCTGA TCGTGCTGGG AAACAAAGTG GACCTCCTGC CCCAGGATGC TCCTGGCTAC 
CGGCAGAGGC TGCGGGAGCG ACTGTGGGAG GACTGTGCCC GCGCCGGGCT CCTGCTGGCC 
CCTGGCCACC AAGGGCCACA GCGCCCCGTC AAGGACGAGC CACAGGACGG GGAGAATCCG 
AATCCGCCGA ACTGGTCCCG CACAGTGGTC AGGGACGTGC GGCTGATCAG CGCCAAGACC 
GGCTATGGAG TGGAAGAGTT GATCTCTGCC CTTCAGCGCT CCTGGCGCTA CCGTGGGGAC 
GTCTACTTAG TGGGCGCCAC CAACGCCGGC AAATCCACTC TCTTTAACAC GCTCCTGGAG 
TCCGATTACT GCACTGCCAA GGGCTCCGAG GCCATCGACA GAGCCACCAT CTCCCCTTGG 
CCAGGTACTA CATTAAACCT TCTGAAGTTT CCTATTTGCA ACCCAACTCC TTACAGAATG 
TTTAAAAGGC ATCAAAGACT TAAAAAAGAT TCAACTCAAG CTGAAGAAGA TCTTAGTGAG 
CAAGAACAAA ATCAGCTTAA TGTCCTCAAA AAGCATGGTT ATGTCGTAGG AAGAGTTGGA 
AGGACATTCT TGTATTCAGA AGAACAGAAG GATAACATTC CCTTTGAGTT TGATGCTGAT 
TCACTTGCCT TTGACATGGA AAATGACCCT GTTATGGGTA CACACAAATC CACCAAACAA 
GTAGAATTGA CTGCACAAGA TGTGAAAGAT GCCCACTGGT TTTATGACAC CCCTGGAATT 
ACAAAAGAAA ATTGTATTTT AAATCTTCTA ACAGAAAAAG AAGTAAATAT TGTTTTGCCA 
ACACAGTCCA TTGTTCCAAG AACTTTTGTG CTTAAACCAG GAATGGTTCT GTTTTTGGGT 
GCTATAGGCC GCATAGATTT CCTGCAGGGA AATCAGTCAG CTTGGTTTAC AGTCGTGGCT 
TCCAACATCC TCCCTGTGCA TATCACCTCC TTGGACAGGG CAGACGCTCT GTATCAGAAG 
CATGCAGGTC ATACGTTACT CCAGATTCCA ATGGGTGGAA AAGAACGAAT GGCAGGATTT 
CCTCCTCTTG TTGCTGAAGA CATTATGTTA AAAGAAGGAC TGGGGGCATC TGAAGCAGTG 
GCCGACATCA AGTTTTCCTC TGCAGGTTGG GTTTCAGTAA CACCTAATTT TAAGGACAGA 
CTGCATCTCC GAGGCTATAC ACCTGAAGGA ACAGTTTTGA CCGTCCGGCC CCCTCTCTTG 
CCATATATTG TTAACATCAA AGGACAGCGC ATCAAGAAAA GTGTGGCCTA TAAAACCAAG 
AAGCCTCCTT CCCTTATGTA CAACGTGAGG AAGAAGAAAG GAAAGATAAA TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115689.1
CDS	22..2118
Translation	MLPARLPFRLLSLFLRGSAPTAARHGLREPLLERRCAAASSFQHSSSLGRELPYDPVDTEGFGEGGDMQERFLFPEYILDPEPQPTREKQLQELQQQQEEEERQRQQRREERRQQNLRARSREHPVVGHPDPALPPSGVNCSGCGAELHCQDAGVPGYLPREKFLRTAEADGGLARTVCQRCWLLSHHRRALRLQVSREQYLELVSAALRRPGPSLVLYMVDLLDLPDALLPDLPALVGPKQLIVLGNKVDLLPQDAPGYRQRLRERLWEDCARAGLLLAPGHQGPQRPVKDEPQDGENPNPPNWSRTVVRDVRLISAKTGYGVEELISALQRSWRYRGDVYLVGATNAGKSTLFNTLLESDYCTAKGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMFKRHQRLKKDSTQAEEDLSEQEQNQLNVLKKHGYVVGRVGRTFLYSEEQKDNIPFEFDADSLAFDMENDPVMGTHKSTKQVELTAQDVKDAHWFYDTPGITKENCILNLLTEKEVNIVLPTQSIVPRTFVLKPGMVLFLGAIGRIDFLQGNQSAWFTVVASNILPVHITSLDRADALYQKHAGHTLLQIPMGGKERMAGFPPLVAEDIMLKEGLGASEAVADIKFSSAGWVSVTPNFKDRLHLRGYTPEGTVLTVRPPLLPYIVNIKGQRIKKSVAYKTKKPPSLMYNVRKKKGKINV

Target ORF information:

RefSeq Version	NM_032313.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide associated 1 (NOA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032313.4

ORF Insert Sequence:

ATGCTGCCCG CTCGCCTACC GTTCAGGCTG CTGAGCCTTT TCCTTCGTGG ATCCGCTCCC 
ACGGCAGCGC GCCATGGCCT CCGGGAGCCG CTCCTGGAGA GGAGGTGCGC TGCCGCCTCC 
TCCTTCCAGC ACTCATCGAG TCTGGGACGC GAGCTTCCTT ATGACCCCGT GGACACGGAG 
GGCTTTGGAG AAGGTGGTGA CATGCAGGAG CGTTTTCTGT TCCCGGAGTA CATCCTGGAT 
CCGGAGCCGC AACCCACCCG CGAAAAGCAG CTGCAGGAGC TCCAGCAACA GCAGGAGGAG 
GAGGAGCGAC AGAGGCAGCA GCGGCGGGAG GAGCGGCGAC AGCAAAACCT ACGGGCCAGG 
TCCCGGGAGC ACCCGGTCGT GGGGCACCCG GACCCGGCAT TGCCGCCCAG CGGCGTGAAC 
TGCTCGGGCT GTGGGGCAGA GCTGCACTGC CAGGACGCCG GAGTGCCCGG CTACCTGCCC 
CGAGAGAAGT TCCTCCGCAC GGCGGAGGCA GACGGCGGGC TGGCACGGAC CGTGTGCCAG 
CGCTGCTGGC TGCTGTCGCA CCACCGGCGC GCTCTACGCC TGCAGGTGAG CCGCGAGCAG 
TACCTGGAGC TGGTGAGCGC CGCGTTGCGG CGGCCCGGCC CCTCCCTGGT GCTCTACATG 
GTGGACCTGC TGGACCTGCC CGACGCCCTG CTGCCCGACT TGCCCGCGCT GGTGGGCCCC 
AAGCAGCTGA TCGTGCTGGG AAACAAAGTG GACCTCCTGC CCCAGGATGC TCCTGGCTAC 
CGGCAGAGGC TGCGGGAGCG ACTGTGGGAG GACTGTGCCC GCGCCGGGCT CCTGCTGGCC 
CCTGGCCACC AAGGGCCACA GCGCCCCGTC AAGGACGAGC CACAGGACGG GGAGAATCCG 
AATCCGCCGA ACTGGTCCCG CACAGTGGTC AGGGACGTGC GGCTGATCAG CGCCAAGACC 
GGCTATGGAG TGGAAGAGTT GATCTCTGCC CTTCAGCGCT CCTGGCGCTA CCGTGGGGAC 
GTCTACTTAG TGGGCGCCAC CAACGCCGGC AAATCCACTC TCTTTAACAC GCTCCTGGAG 
TCCGATTACT GCACTGCCAA GGGCTCCGAG GCCATCGACA GAGCCACCAT CTCCCCTTGG 
CCAGGTACTA CATTAAACCT TCTGAAGTTT CCTATTTGCA ACCCAACTCC TTACAGAATG 
TTTAAAAGGC ATCAAAGACT TAAAAAAGAT TCAACTCAAG CTGAAGAAGA TCTTAGTGAG 
CAAGAACAAA ATCAGCTTAA TGTCCTCAAA AAGCATGGTT ATGTCGTAGG AAGAGTTGGA 
AGGACATTCT TGTATTCAGA AGAACAGAAG GATAACATTC CCTTTGAGTT TGATGCTGAT 
TCACTTGCCT TTGACATGGA AAATGACCCT GTTATGGGTA CACACAAATC CACCAAACAA 
GTAGAATTGA CTGCACAAGA TGTGAAAGAT GCCCACTGGT TTTATGACAC CCCTGGAATT 
ACAAAAGAAA ATTGTATTTT AAATCTTCTA ACAGAAAAAG AAGTAAATAT TGTTTTGCCA 
ACACAGTCCA TTGTTCCAAG AACTTTTGTG CTTAAACCAG GAATGGTTCT GTTTTTGGGT 
GCTATAGGCC GCATAGATTT CCTGCAGGGA AATCAGTCAG CTTGGTTTAC AGTCGTGGCT 
TCCAACATCC TCCCTGTGCA TATCACCTCC TTGGACAGGG CAGACGCTCT GTATCAGAAG 
CATGCAGGTC ATACGTTACT CCAGATTCCA ATGGGTGGAA AAGAACGAAT GGCAGGATTT 
CCTCCTCTTG TTGCTGAAGA CATTATGTTA AAAGAAGGAC TGGGGGCATC TGAAGCAGTG 
GCCGACATCA AGTTTTCCTC TGCAGGTTGG GTTTCAGTAA CACCTAATTT TAAGGACAGA 
CTGCATCTCC GAGGCTATAC ACCTGAAGGA ACAGTTTTGA CCGTCCGGCC CCCTCTCTTG 
CCATATATTG TTAACATCAA AGGACAGCGC ATCAAGAAAA GTGTGGCCTA TAAAACCAAG 
AAGCCTCCTT CCCTTATGTA CAACGTGAGG AAGAAGAAAG GAAAGATAAA TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NOA1, a novel ClpXP substrate, takes an unexpected nuclear detour prior to mitochondrial import.
PloS one9(7)e103141(2014)
Al-Furoukh N,Kardon JR,Kr?ger M,Szibor M,Baker TA,Braun T

Human C4orf14 interacts with the mitochondrial nucleoid and is involved in the biogenesis of the small mitochondrial ribosomal subunit.
Nucleic acids research40(13)6097-108(2012 Jul)
He J,Cooper HM,Reyes A,Di Re M,Kazak L,Wood SR,Mao CC,Fearnley IM,Walker JE,Holt IJ

Nitric oxide-associated protein 1 (NOA1) is necessary for oxygen-dependent regulation of mitochondrial respiratory complexes.
The Journal of biological chemistry286(37)32086-93(2011 Sep)
Heidler J,Al-Furoukh N,Kukat C,Salwig I,Ingelmann ME,Seibel P,Kr?ger M,Holtz J,Wittig I,Braun T,Szibor M

Genome-wide association study identifies two susceptibility loci for exudative age-related macular degeneration in the Japanese population.
Nature genetics43(10)1001-4(2011 Sep)
Arakawa S,Takahashi A,Ashikawa K,Hosono N,Aoi T,Yasuda M,Oshima Y,Yoshida S,Enaida H,Tsuchihashi T,Mori K,Honda S,Negi A,Arakawa A,Kadonosono K,Kiyohara Y,Kamatani N,Nakamura Y,Ishibashi T,Kubo M

NOA1 is an essential GTPase required for mitochondrial protein synthesis.
Molecular biology of the cell22(1)1-11(2011 Jan)
Kolanczyk M,Pech M,Zemojtel T,Yamamoto H,Mikula I,Calvaruso MA,van den Brand M,Richter R,Fischer B,Ritz A,Kossler N,Thurisch B,Spoerle R,Smeitink J,Kornak U,Chan D,Vingron M,Martasek P,Lightowlers RN,Nijtmans L,Schuelke M,Nierhaus KH,Mundlos S

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NOA1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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