POMK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POMK
Entrez Gene ID	84197
Full Name	protein-O-mannose kinase
Synonyms	MDDGA12,MDDGC12,SGK196
General protein information	Preferred Names protein-O-mannose kinase Names protein O-mannose kinase Sugen kinase 196 probable inactive protein kinase-like protein SgK196 protein kinase-like protein SgK196
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p11.21
Summary	This gene encodes a protein that may be involved in the presentation of the laminin-binding O-linked carbohydrate chain of alpha-dystroglycan (a-DG), which forms transmembrane linkages between the extracellular matrix and the exoskeleton. Some pathogens use this O-linked carbohydrate unit for host entry. Loss of function compound heterozygous mutations in this gene were found in a human patient affected by the Walker-Warburg syndrome (WWS) phenotype. Mice lacking this gene contain misplaced neurons (heterotopia) in some regions of the brain, possibly from defects in neuronal migration. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013].
Disorder MIM:	615247
Disorder Html:	Muscular dystrophy-dystroglycanopathy (congenital with brain and eye anomalies), type A, 12, 615249 (3); ?Muscular dystrophy-dystroglycanopathy (limb-girdle), type C, 12, 616094 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011544669.1	XP_011542971.1	protein O-mannose kinase isoform X1
NM_032237.4	NP_115613.1	protein O-mannose kinase
XM_011544668.1	XP_011542970.1	protein O-mannose kinase isoform X1
NM_032237.5	NP_115613.1	protein O-mannose kinase
NM_001277971.1	NP_001264900.1	protein O-mannose kinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	POMK	NP_001264900.1
Pan troglodytes (chimpanzee)	SGK196	XP_519740.2
Mus musculus (house mouse)	Pomk	NP_083313.1
Xenopus tropicalis (tropical clawed frog)	LOC100497421	XP_004911106.1
Canis lupus familiaris (dog)	SGK196	XP_005629839.1
Rattus norvegicus (Norway rat)	Pomk	NP_001020054.1
Danio rerio (zebrafish)	pomk	NP_001007415.1
Gallus gallus (chicken)	SGK196	NP_001026303.1
	LOC706318	XP_001099665.1
Bos taurus (cattle)	LOC514490	NP_001091496.1

Gene Ontology
Bibliography

Related articles in PubMed

POMK mutations disrupt muscle development leading to a spectrum of neuromuscular presentations.
Di Costanzo S, Balasubramanian A, Pond HL, Rozkalne A, Pantaleoni C, Saredi S, Gupta VA, Sunu CM, Yu TW, Kang PB, Salih MA, Mora M, Gussoni E, Walsh CA, Manzini MC
Human molecular genetics23(21)5781-92(2014 Nov)

POMK mutation in a family with congenital muscular dystrophy with merosin deficiency, hypomyelination, mild hearing deficit and intellectual disability.
von Renesse A, Petkova MV, L?tzkendorf S, Heinemeyer J, Gill E, H?bner C, von Moers A, Stenzel W, Schuelke M
Journal of medical genetics51(4)275-82(2014 Apr)

SGK196 is a glycosylation-specific O-mannose kinase required for dystroglycan function.
Yoshida-Moriguchi T, Willer T, Anderson ME, Venzke D, Whyte T, Muntoni F, Lee H, Nelson SF, Yu L, Campbell KP
Science (New York, N.Y.)341(6148)896-9(2013 Aug)

Deciphering the glycosylome of dystroglycanopathies using haploid screens for lassa virus entry.
Jae LT, Raaben M, Riemersma M, van Beusekom E, Blomen VA, Velds A, Kerkhoven RM, Carette JE, Topaloglu H, Meinecke P, Wessels MW, Lefeber DJ, Whelan SP, van Bokhoven H, Brummelkamp TR
Science (New York, N.Y.)340(6131)479-83(2013 Apr)

Congenital hydrocephalus in genetically engineered mice.
Vogel P, Read RW, Hansen GM, Payne BJ, Small D, Sands AT, Zambrowicz BP
Veterinary pathology49(1)166-81(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

POMK mutations disrupt muscle development leading to a spectrum of neuromuscular presentations.
Title: POMK mutations disrupt muscle development leading to a spectrum of neuromuscular presentations.

Homozygous truncating mutations in POMK lead to congenital muscular dystrophies with secondary merosin deficiency, hypomyelination and intellectual disability.
Title: POMK mutation in a family with congenital muscular dystrophy with merosin deficiency, hypomyelination, mild hearing deficit and intellectual disability.

These findings suggest how mutations in GTDC2, B3GALNT2, and SGK196 disrupt dystroglycan receptor function and lead to congenital muscular dystrophy.
Title: SGK196 is a glycosylation-specific O-mannose kinase required for dystroglycan function.

The following POMK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POMK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15079	NM_001277971.1 Latest version!	Homo sapiens protein O-mannose kinase (POMK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu15079	NM_032237.5 Latest version!	Homo sapiens protein O-mannose kinase (POMK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu15079	XM_011544669.1 Latest version!	Homo sapiens protein-O-mannose kinase (POMK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu15079	XM_011544668.1 Latest version!	Homo sapiens protein-O-mannose kinase (POMK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu15079	NM_032237.4	Homo sapiens protein-O-mannose kinase (POMK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POMK ( NM_032237.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115613.1 Homo sapiens protein-O-mannose kinase (POMK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15079
Clone ID Related Accession (Same CDS sequence)	XM_011544669.1 , NM_032237.4 , XM_011544668.1 , NM_032237.5 , NM_001277971.1
Accession Version	NM_032237.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	protein O-mannose kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA074115.1, DA330953.1, BC101548.1, EH337134.1, BQ365114.1 and AC113191.13. This sequence is a reference standard in the RefSeqGene project. On May 3, 2013 this sequence version replaced NM_032237.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.121882.1, SRR1660805.126735.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115613.1
CDS	268..1320
Translation	MEKQPQNSRRGLAPREVPPAVGLLLIMALMNTLLYLCLDHFFIAPRQSTVDPTHCPYGHFRIGQMKNCSPWLSCEELRTEVRQLKRVGEGAVKRVFLSEWKEHKVALSQLTSLEMKDDFLHGLQMLKSLQGTHVVTLLGYCEDDNTMLTEYHPLGSLSNLEETLNLSKYQNVNTWQHRLELAMDYVSIINYLHHSPVGTRVMCDSNDLPKTLSQYLLTSNFSILANDLDALPLVNHSSGMLVKCGHRELHGDFVAPEQLWPYGEDVPFHDDLMPSYDEKIDIWKIPDISSFLLGHIEGSDMVRFHLFDIHKACKSQTPSERPTAQDVLETYQKVLDTLRDAMMSQAREML

Target ORF information:

RefSeq Version	NM_032237.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein-O-mannose kinase (POMK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032237.4

ORF Insert Sequence:

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMK ( NM_032237.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_115613.1 Homo sapiens protein O-mannose kinase (POMK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15079
Clone ID Related Accession (Same CDS sequence)	XM_011544669.1 , NM_032237.4 , XM_011544668.1 , NM_032237.5 , NM_001277971.1
Accession Version	NM_032237.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	protein O-mannose kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA330953.1, BC101548.1, EH337134.1, BQ365114.1 and AC113191.13. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_032237.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.123243.1, SRR1803615.222841.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000331373.10/ ENSP00000331258.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115613.1
CDS	259..1311
Translation	MEKQPQNSRRGLAPREVPPAVGLLLIMALMNTLLYLCLDHFFIAPRQSTVDPTHCPYGHFRIGQMKNCSPWLSCEELRTEVRQLKRVGEGAVKRVFLSEWKEHKVALSQLTSLEMKDDFLHGLQMLKSLQGTHVVTLLGYCEDDNTMLTEYHPLGSLSNLEETLNLSKYQNVNTWQHRLELAMDYVSIINYLHHSPVGTRVMCDSNDLPKTLSQYLLTSNFSILANDLDALPLVNHSSGMLVKCGHRELHGDFVAPEQLWPYGEDVPFHDDLMPSYDEKIDIWKIPDISSFLLGHIEGSDMVRFHLFDIHKACKSQTPSERPTAQDVLETYQKVLDTLRDAMMSQAREML

Target ORF information:

RefSeq Version	NM_032237.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein O-mannose kinase (POMK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032237.5

ORF Insert Sequence:

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMK ( NM_001277971.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264900.1 Homo sapiens protein O-mannose kinase (POMK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15079
Clone ID Related Accession (Same CDS sequence)	XM_011544669.1 , NM_032237.4 , XM_011544668.1 , NM_032237.5 , NM_001277971.1
Accession Version	NM_001277971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Homo sapiens(human)
Product	protein O-mannose kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA074115.1, DA818523.1, BC113703.1, EH337134.1, BQ365114.1 and AC113191.13. Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.185541.1, SRR1803611.21840.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264900.1
CDS	172..1224
Translation	MEKQPQNSRRGLAPREVPPAVGLLLIMALMNTLLYLCLDHFFIAPRQSTVDPTHCPYGHFRIGQMKNCSPWLSCEELRTEVRQLKRVGEGAVKRVFLSEWKEHKVALSQLTSLEMKDDFLHGLQMLKSLQGTHVVTLLGYCEDDNTMLTEYHPLGSLSNLEETLNLSKYQNVNTWQHRLELAMDYVSIINYLHHSPVGTRVMCDSNDLPKTLSQYLLTSNFSILANDLDALPLVNHSSGMLVKCGHRELHGDFVAPEQLWPYGEDVPFHDDLMPSYDEKIDIWKIPDISSFLLGHIEGSDMVRFHLFDIHKACKSQTPSERPTAQDVLETYQKVLDTLRDAMMSQAREML

Target ORF information:

RefSeq Version	NM_001277971.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein O-mannose kinase (POMK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277971.1

ORF Insert Sequence:

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMK ( XM_011544669.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542971.1 Homo sapiens protein-O-mannose kinase (POMK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu15079
Clone ID Related Accession (Same CDS sequence)	XM_011544669.1 , NM_032237.4 , XM_011544668.1 , NM_032237.5 , NM_001277971.1
Accession Version	XM_011544669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	protein O-mannose kinase isoform X1
Comment

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542971.1
Translation	MEKQPQNSRRGLAPREVPPAVGLLLIMALMNTLLYLCLDHFFIAPRQSTVDPTHCPYGHFRIGQMKNCSPWLSCEELRTEVRQLKRVGEGAVKRVFLSEWKEHKVALSQLTSLEMKDDFLHGLQMLKSLQGTHVVTLLGYCEDDNTMLTEYHPLGSLSNLEETLNLSKYQNVNTWQHRLELAMDYVSIINYLHHSPVGTRVMCDSNDLPKTLSQYLLTSNFSILANDLDALPLVNHSSGMLVKCGHRELHGDFVAPEQLWPYGEDVPFHDDLMPSYDEKIDIWKIPDISSFLLGHIEGSDMVRFHLFDIHKACKSQTPSERPTAQDVLETYQKVLDTLRDAMMSQAREML

Target ORF information:

RefSeq Version	XM_011544669.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens protein-O-mannose kinase (POMK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544669.1

ORF Insert Sequence:

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMK ( XM_011544668.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542970.1 Homo sapiens protein-O-mannose kinase (POMK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu15079
Clone ID Related Accession (Same CDS sequence)	XM_011544669.1 , NM_032237.4 , XM_011544668.1 , NM_032237.5 , NM_001277971.1
Accession Version	XM_011544668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	protein O-mannose kinase isoform X1
Comment

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542970.1
Translation	MEKQPQNSRRGLAPREVPPAVGLLLIMALMNTLLYLCLDHFFIAPRQSTVDPTHCPYGHFRIGQMKNCSPWLSCEELRTEVRQLKRVGEGAVKRVFLSEWKEHKVALSQLTSLEMKDDFLHGLQMLKSLQGTHVVTLLGYCEDDNTMLTEYHPLGSLSNLEETLNLSKYQNVNTWQHRLELAMDYVSIINYLHHSPVGTRVMCDSNDLPKTLSQYLLTSNFSILANDLDALPLVNHSSGMLVKCGHRELHGDFVAPEQLWPYGEDVPFHDDLMPSYDEKIDIWKIPDISSFLLGHIEGSDMVRFHLFDIHKACKSQTPSERPTAQDVLETYQKVLDTLRDAMMSQAREML

Target ORF information:

RefSeq Version	XM_011544668.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens protein-O-mannose kinase (POMK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544668.1

ORF Insert Sequence:

ATGGAAAAGC AGCCCCAGAA CAGCAGGAGA GGCCTCGCCC CCCGAGAGGT GCCGCCAGCT 
GTTGGGCTGC TGCTGATCAT GGCCCTGATG AATACTCTGC TCTACCTCTG CCTCGACCAC 
TTCTTCATCG CTCCTCGACA ATCCACTGTG GACCCCACAC ACTGTCCCTA TGGTCACTTC 
AGGATAGGAC AGATGAAAAA CTGCTCACCT TGGCTGTCCT GCGAGGAGCT GAGAACAGAA 
GTGAGACAGC TGAAGCGTGT TGGGGAAGGA GCTGTAAAGA GAGTCTTTCT GTCTGAGTGG 
AAGGAGCACA AAGTTGCACT CTCACAGCTC ACCAGCCTGG AGATGAAAGA TGATTTCCTC 
CATGGACTGC AGATGCTGAA ATCTCTCCAA GGCACACATG TTGTCACGCT GCTTGGCTAT 
TGTGAGGATG ACAACACTAT GCTTACTGAA TATCACCCTC TAGGTTCCTT GAGTAACCTG 
GAAGAAACAC TAAACCTTTC AAAGTACCAA AATGTGAACA CGTGGCAGCA CAGGCTGGAG 
CTGGCCATGG ACTATGTCAG CATCATTAAT TACCTGCACC ACAGCCCTGT GGGCACACGG 
GTCATGTGCG ACTCCAACGA CCTGCCGAAG ACACTGTCCC AGTATCTGCT AACAAGCAAC 
TTCAGCATTT TGGCAAATGA CTTGGACGCC TTACCCCTGG TGAACCACAG CTCCGGGATG 
CTGGTGAAGT GCGGCCACAG GGAGCTGCAT GGGGATTTCG TGGCTCCAGA GCAACTGTGG 
CCCTATGGAG AGGACGTGCC TTTCCACGAT GATCTCATGC CCTCATATGA TGAGAAGATT 
GACATTTGGA AGATCCCAGA CATCTCCAGT TTCCTTCTGG GGCACATTGA AGGGAGTGAT 
ATGGTCCGAT TCCATTTGTT TGATATTCAC AAAGCATGCA AGAGCCAGAC TCCCTCAGAA 
AGACCCACTG CCCAGGACGT TCTGGAGACC TACCAGAAGG TCTTGGATAC ACTTAGAGAT 
GCCATGATGT CTCAGGCAAG AGAGATGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

POMK mutations disrupt muscle development leading to a spectrum of neuromuscular presentations.
Human molecular genetics23(21)5781-92(2014 Nov)
Di Costanzo S,Balasubramanian A,Pond HL,Rozkalne A,Pantaleoni C,Saredi S,Gupta VA,Sunu CM,Yu TW,Kang PB,Salih MA,Mora M,Gussoni E,Walsh CA,Manzini MC

POMK mutation in a family with congenital muscular dystrophy with merosin deficiency, hypomyelination, mild hearing deficit and intellectual disability.
Journal of medical genetics51(4)275-82(2014 Apr)
von Renesse A,Petkova MV,L?tzkendorf S,Heinemeyer J,Gill E,H?bner C,von Moers A,Stenzel W,Schuelke M

SGK196 is a glycosylation-specific O-mannose kinase required for dystroglycan function.
Science (New York, N.Y.)341(6148)896-9(2013 Aug)
Yoshida-Moriguchi T,Willer T,Anderson ME,Venzke D,Whyte T,Muntoni F,Lee H,Nelson SF,Yu L,Campbell KP

Deciphering the glycosylome of dystroglycanopathies using haploid screens for lassa virus entry.
Science (New York, N.Y.)340(6131)479-83(2013 Apr)
Jae LT,Raaben M,Riemersma M,van Beusekom E,Blomen VA,Velds A,Kerkhoven RM,Carette JE,Topaloglu H,Meinecke P,Wessels MW,Lefeber DJ,Whelan SP,van Bokhoven H,Brummelkamp TR

Congenital hydrocephalus in genetically engineered mice.
Veterinary pathology49(1)166-81(2012 Jan)
Vogel P,Read RW,Hansen GM,Payne BJ,Small D,Sands AT,Zambrowicz BP

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POMK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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