CASP6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CASP6
Entrez Gene ID	839
Full Name	caspase 6
Synonyms	MCH2
General protein information	Preferred Names caspase 6 Names caspase-6 apoptotic protease MCH-2 caspase 6, apoptosis-related cysteine peptidase caspase 6, apoptosis-related cysteine protease
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q25
Summary	This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015].
Disorder MIM:	601532

mRNA and Protein(s)

mRNA	Protein	Name
NM_001226.3	NP_001217.2	caspase-6 isoform alpha precursor
NM_001226.4	NP_001217.2	caspase-6 isoform alpha precursor
NM_032992.3	NP_116787.1	caspase-6 isoform beta
NM_032992.2	NP_116787.1	caspase-6 isoform beta

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Casp6	NP_033941.3
Bos taurus (cattle)	CASP6	NP_001030496.1
Gallus gallus (chicken)	CASP6	NP_990057.1
Xenopus tropicalis (tropical clawed frog)	casp6	NP_001011068.1
Homo sapiens (human)	CASP6	NP_001217.2
Pan troglodytes (chimpanzee)	CASP6	XP_001136602.2
	LOC698788	XP_002804209.1
Danio rerio (zebrafish)	casp6	NP_001018333.1
Canis lupus familiaris (dog)	CASP6	XP_545022.3
Rattus norvegicus (Norway rat)	Casp6	NP_001258913.1

Gene Ontology
Bibliography

Related articles in PubMed

Multiple proteolytic events in caspase-6 self-activation impact conformations of discrete structural regions.
Dagbay KB, Hardy JA
Proceedings of the National Academy of Sciences of the United States of America114(38)E7977-E7986(2017 Sep)

Tumor-Associated Mutations in Caspase-6 Negatively Impact Catalytic Efficiency.
Dagbay KB, Hill ME, Barrett E, Hardy JA
Biochemistry56(34)4568-4577(2017 08)

Ceramide phosphoethanolamine synthase SMSr is a target of caspase-6 during apoptotic cell death.
Cabukusta B, Nettebrock NT, Kol M, Hilderink A, Tafesse FG, Holthuis JCM
Bioscience reports37(4)(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that caspase-6 deactivating mutations may contribute to multifactorial carcinogenic transformations.
Title: Tumor-Associated Mutations in Caspase-6 Negatively Impact Catalytic Efficiency.

Caspase-6 undergoes helix-strand transition upon substrate binding. Caspase-6 shows distinctive conformational dynamics in its 130's region Local pKa Values of Key Amino Acid Residues within the 130's Region Vary between the Unliganded (Helical) and the VEID-bound (Strand) States of Caspase-6 .
Title: Caspase-6 Undergoes a Distinct Helix-Strand Interconversion upon Substrate Binding.

Results support the possibility that the Casp6 activity in the anterior olfactory nucleus of the olfactory bulb reflects degeneration in the entorhinal cortex and suggest that Casp6 activity in the olfactory bulb could represent degeneration associated with cognitive decline and early Alzheimer disease.
Title: Increased Caspase-6 activity in the human anterior olfactory nuclei of the olfactory bulb is associated with cognitive impairment.

Following specific binding to and internalization into HER2-overexpressing tumor cells, the e23sFv-Fdt-casp6 protein induced tumor cell apoptosis and inhibited the proliferation of HER2-overexpressing A172 and U251MG cells in vitro, but not in U87MG cells with undetectable HER2
Title: HER2-targeted recombinant protein immuno-caspase-6 effectively induces apoptosis in HER2-overexpressing GBM cells in?vitro and in?vivo.

Results identified novel members of the CASP6 interactome and demonstrate that a number of them are involved in key signaling pathways observed in neurodegenerative diseases.
Title: Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.

The following CASP6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASP6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19575	NM_001226.4 Latest version!	Homo sapiens caspase 6 (CASP6), transcript variant alpha, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu05221	NM_032992.3 Latest version!	Homo sapiens caspase 6 (CASP6), transcript variant beta, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OHu19575	NM_001226.3	Homo sapiens caspase 6 (CASP6), transcript variant alpha, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu05221	NM_032992.2	Homo sapiens caspase 6 (CASP6), transcript variant beta, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CASP6 ( NM_001226.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001217.2 Homo sapiens caspase 6 (CASP6), transcript variant alpha, mRNA.

Price & Availability↑

CloneID	OHu19575
Clone ID Related Accession (Same CDS sequence)	NM_001226.3 , NM_001226.4
Accession Version	NM_001226.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	caspase-6 isoform alpha precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U20536.1, BC000305.2 and AC004067.1. This sequence is a reference standard in the RefSeqGene project. On Aug 19, 2005 this sequence version replaced NM_001226.2. Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U20536.1, SRR1163655.361213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2153347 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTCGG CCTCGGGGCT CCGCAGGGGG CACCCGGCAG GTGGGGAAGA AAACATGACA 
GAAACAGATG CCTTCTATAA AAGAGAAATG TTTGATCCGG CAGAAAAGTA CAAAATGGAC 
CACAGGAGGA GAGGAATTGC TTTAATCTTC AATCATGAGA GGTTCTTTTG GCACTTAACA 
CTGCCAGAAA GGCGGGGCAC CTGCGCAGAT AGAGACAATC TTACCCGCAG GTTTTCAGAT 
CTAGGATTTG AAGTGAAATG CTTTAATGAT CTTAAAGCAG AAGAACTACT GCTCAAAATT 
CATGAGGTGT CAACTGTTAG CCACGCAGAT GCCGATTGCT TTGTGTGTGT CTTCCTGAGC 
CATGGCGAAG GCAATCACAT TTATGCATAT GATGCTAAAA TCGAAATTCA GACATTAACT 
GGCTTGTTCA AAGGAGACAA GTGTCACAGC CTGGTTGGAA AACCCAAGAT ATTTATCATT 
CAGGCATGTC GGGGAAACCA GCACGATGTG CCAGTCATTC CTTTGGATGT AGTAGATAAT 
CAGACAGAGA AGTTGGACAC CAACATAACT GAGGTGGATG CAGCCTCCGT TTACACGCTG 
CCTGCTGGAG CTGACTTCCT CATGTGTTAC TCTGTTGCAG AAGGATATTA TTCTCACCGG 
GAAACTGTGA ACGGCTCATG GTACATTCAA GATTTGTGTG AGATGTTGGG AAAATATGGC 
TCCTCCTTAG AGTTCACAGA ACTCCTCACA CTGGTGAACA GGAAAGTTTC TCAGCGCCGA 
GTGGACTTTT GCAAAGACCC AAGTGCAATT GGAAAGAAGC AGGTTCCCTG TTTTGCCTCA 
ATGCTAACTA AAAAGCTGCA TTTCTTTCCA AAATCTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001217.2
CDS	79..960
Translation	MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIFNHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLHFFPKSN

Target ORF information:

RefSeq Version	NM_001226.3
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 6 (CASP6), transcript variant alpha, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001226.3

ORF Insert Sequence:

ATGAGCTCGG CCTCGGGGCT CCGCAGGGGG CACCCGGCAG GTGGGGAAGA AAACATGACA 
GAAACAGATG CCTTCTATAA AAGAGAAATG TTTGATCCGG CAGAAAAGTA CAAAATGGAC 
CACAGGAGGA GAGGAATTGC TTTAATCTTC AATCATGAGA GGTTCTTTTG GCACTTAACA 
CTGCCAGAAA GGCGGGGCAC CTGCGCAGAT AGAGACAATC TTACCCGCAG GTTTTCAGAT 
CTAGGATTTG AAGTGAAATG CTTTAATGAT CTTAAAGCAG AAGAACTACT GCTCAAAATT 
CATGAGGTGT CAACTGTTAG CCACGCAGAT GCCGATTGCT TTGTGTGTGT CTTCCTGAGC 
CATGGCGAAG GCAATCACAT TTATGCATAT GATGCTAAAA TCGAAATTCA GACATTAACT 
GGCTTGTTCA AAGGAGACAA GTGTCACAGC CTGGTTGGAA AACCCAAGAT ATTTATCATT 
CAGGCATGTC GGGGAAACCA GCACGATGTG CCAGTCATTC CTTTGGATGT AGTAGATAAT 
CAGACAGAGA AGTTGGACAC CAACATAACT GAGGTGGATG CAGCCTCCGT TTACACGCTG 
CCTGCTGGAG CTGACTTCCT CATGTGTTAC TCTGTTGCAG AAGGATATTA TTCTCACCGG 
GAAACTGTGA ACGGCTCATG GTACATTCAA GATTTGTGTG AGATGTTGGG AAAATATGGC 
TCCTCCTTAG AGTTCACAGA ACTCCTCACA CTGGTGAACA GGAAAGTTTC TCAGCGCCGA 
GTGGACTTTT GCAAAGACCC AAGTGCAATT GGAAAGAAGC AGGTTCCCTG TTTTGCCTCA 
ATGCTAACTA AAAAGCTGCA TTTCTTTCCA AAATCTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP6 ( NM_001226.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001217.2 Homo sapiens caspase 6 (CASP6), transcript variant alpha, mRNA.

Price & Availability↑

CloneID	OHu19575
Clone ID Related Accession (Same CDS sequence)	NM_001226.3 , NM_001226.4
Accession Version	NM_001226.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	caspase-6 isoform alpha precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U20536.1, BC000305.2 and AC004067.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001226.3. Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1083565.1, SRR5189661.121761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2153347 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265164.7/ ENSP00000265164.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCTCGG CCTCGGGGCT CCGCAGGGGG CACCCGGCAG GTGGGGAAGA AAACATGACA 
GAAACAGATG CCTTCTATAA AAGAGAAATG TTTGATCCGG CAGAAAAGTA CAAAATGGAC 
CACAGGAGGA GAGGAATTGC TTTAATCTTC AATCATGAGA GGTTCTTTTG GCACTTAACA 
CTGCCAGAAA GGCGGGGCAC CTGCGCAGAT AGAGACAATC TTACCCGCAG GTTTTCAGAT 
CTAGGATTTG AAGTGAAATG CTTTAATGAT CTTAAAGCAG AAGAACTACT GCTCAAAATT 
CATGAGGTGT CAACTGTTAG CCACGCAGAT GCCGATTGCT TTGTGTGTGT CTTCCTGAGC 
CATGGCGAAG GCAATCACAT TTATGCATAT GATGCTAAAA TCGAAATTCA GACATTAACT 
GGCTTGTTCA AAGGAGACAA GTGTCACAGC CTGGTTGGAA AACCCAAGAT ATTTATCATT 
CAGGCATGTC GGGGAAACCA GCACGATGTG CCAGTCATTC CTTTGGATGT AGTAGATAAT 
CAGACAGAGA AGTTGGACAC CAACATAACT GAGGTGGATG CAGCCTCCGT TTACACGCTG 
CCTGCTGGAG CTGACTTCCT CATGTGTTAC TCTGTTGCAG AAGGATATTA TTCTCACCGG 
GAAACTGTGA ACGGCTCATG GTACATTCAA GATTTGTGTG AGATGTTGGG AAAATATGGC 
TCCTCCTTAG AGTTCACAGA ACTCCTCACA CTGGTGAACA GGAAAGTTTC TCAGCGCCGA 
GTGGACTTTT GCAAAGACCC AAGTGCAATT GGAAAGAAGC AGGTTCCCTG TTTTGCCTCA 
ATGCTAACTA AAAAGCTGCA TTTCTTTCCA AAATCTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001217.2
CDS	51..932
Translation	MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIFNHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLHFFPKSN

Target ORF information:

RefSeq Version	NM_001226.4
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 6 (CASP6), transcript variant alpha, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001226.4

ORF Insert Sequence:

ATGAGCTCGG CCTCGGGGCT CCGCAGGGGG CACCCGGCAG GTGGGGAAGA AAACATGACA 
GAAACAGATG CCTTCTATAA AAGAGAAATG TTTGATCCGG CAGAAAAGTA CAAAATGGAC 
CACAGGAGGA GAGGAATTGC TTTAATCTTC AATCATGAGA GGTTCTTTTG GCACTTAACA 
CTGCCAGAAA GGCGGGGCAC CTGCGCAGAT AGAGACAATC TTACCCGCAG GTTTTCAGAT 
CTAGGATTTG AAGTGAAATG CTTTAATGAT CTTAAAGCAG AAGAACTACT GCTCAAAATT 
CATGAGGTGT CAACTGTTAG CCACGCAGAT GCCGATTGCT TTGTGTGTGT CTTCCTGAGC 
CATGGCGAAG GCAATCACAT TTATGCATAT GATGCTAAAA TCGAAATTCA GACATTAACT 
GGCTTGTTCA AAGGAGACAA GTGTCACAGC CTGGTTGGAA AACCCAAGAT ATTTATCATT 
CAGGCATGTC GGGGAAACCA GCACGATGTG CCAGTCATTC CTTTGGATGT AGTAGATAAT 
CAGACAGAGA AGTTGGACAC CAACATAACT GAGGTGGATG CAGCCTCCGT TTACACGCTG 
CCTGCTGGAG CTGACTTCCT CATGTGTTAC TCTGTTGCAG AAGGATATTA TTCTCACCGG 
GAAACTGTGA ACGGCTCATG GTACATTCAA GATTTGTGTG AGATGTTGGG AAAATATGGC 
TCCTCCTTAG AGTTCACAGA ACTCCTCACA CTGGTGAACA GGAAAGTTTC TCAGCGCCGA 
GTGGACTTTT GCAAAGACCC AAGTGCAATT GGAAAGAAGC AGGTTCCCTG TTTTGCCTCA 
ATGCTAACTA AAAAGCTGCA TTTCTTTCCA AAATCTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP6 ( NM_032992.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116787.1 Homo sapiens caspase 6 (CASP6), transcript variant beta, mRNA.

Price & Availability↑

CloneID	OHu05221
Clone ID Related Accession (Same CDS sequence)	NM_032992.3 , NM_032992.2
Accession Version	NM_032992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	caspase-6 isoform beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U20536.1, U20537.1, BM837197.1 and AC004067.1. On Jun 1, 2019 this sequence version replaced NM_032992.2. Transcript Variant: This variant (beta) lacks several exons in the coding region but maintains the reading frame, compared to variant alpha. It encodes isoform beta which is shorter than isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.100513.1, U20537.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTCGG CCTCGGGGCT CCGCAGGGGG CACCCGGCAG TGTCAACTGT TAGCCACGCA 
GATGCCGATT GCTTTGTGTG TGTCTTCCTG AGCCATGGCG AAGGCAATCA CATTTATGCA 
TATGATGCTA AAATCGAAAT TCAGACATTA ACTGGCTTGT TCAAAGGAGA CAAGTGTCAC 
AGCCTGGTTG GAAAACCCAA GATATTTATC ATTCAGGCAT GTCGGGGAAA CCAGCACGAT 
GTGCCAGTCA TTCCTTTGGA TGTAGTAGAT AATCAGACAG AGAAGTTGGA CACCAACATA 
ACTGAGGTGG ATGCAGCCTC CGTTTACACG CTGCCTGCTG GAGCTGACTT CCTCATGTGT 
TACTCTGTTG CAGAAGGATA TTATTCTCAC CGGGAAACTG TGAACGGCTC ATGGTACATT 
CAAGATTTGT GTGAGATGTT GGGAAAATAT GGCTCCTCCT TAGAGTTCAC AGAACTCCTC 
ACACTGGTGA ACAGGAAAGT TTCTCAGCGC CGAGTGGACT TTTGCAAAGA CCCAAGTGCA 
ATTGGAAAGA AGCAGGTTCC CTGTTTTGCC TCAATGCTAA CTAAAAAGCT GCATTTCTTT 
CCAAAATCTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116787.1
CDS	51..665
Translation	MSSASGLRRGHPAVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLHFFPKSN

Target ORF information:

RefSeq Version	NM_032992.3
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 6 (CASP6), transcript variant beta, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032992.3

ORF Insert Sequence:

ATGAGCTCGG CCTCGGGGCT CCGCAGGGGG CACCCGGCAG TGTCAACTGT TAGCCACGCA 
GATGCCGATT GCTTTGTGTG TGTCTTCCTG AGCCATGGCG AAGGCAATCA CATTTATGCA 
TATGATGCTA AAATCGAAAT TCAGACATTA ACTGGCTTGT TCAAAGGAGA CAAGTGTCAC 
AGCCTGGTTG GAAAACCCAA GATATTTATC ATTCAGGCAT GTCGGGGAAA CCAGCACGAT 
GTGCCAGTCA TTCCTTTGGA TGTAGTAGAT AATCAGACAG AGAAGTTGGA CACCAACATA 
ACTGAGGTGG ATGCAGCCTC CGTTTACACG CTGCCTGCTG GAGCTGACTT CCTCATGTGT 
TACTCTGTTG CAGAAGGATA TTATTCTCAC CGGGAAACTG TGAACGGCTC ATGGTACATT 
CAAGATTTGT GTGAGATGTT GGGAAAATAT GGCTCCTCCT TAGAGTTCAC AGAACTCCTC 
ACACTGGTGA ACAGGAAAGT TTCTCAGCGC CGAGTGGACT TTTGCAAAGA CCCAAGTGCA 
ATTGGAAAGA AGCAGGTTCC CTGTTTTGCC TCAATGCTAA CTAAAAAGCT GCATTTCTTT 
CCAAAATCTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP6 ( NM_032992.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_116787.1 Homo sapiens caspase 6 (CASP6), transcript variant beta, mRNA.

Price & Availability↑

CloneID	OHu05221
Clone ID Related Accession (Same CDS sequence)	NM_032992.3 , NM_032992.2
Accession Version	NM_032992.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-10
Organism	Homo sapiens(human)
Product	caspase-6 isoform beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U20536.1, U20537.1, BM837197.1 and AC004067.1. On Aug 19, 2005 this sequence version replaced NM_032992.1. Transcript Variant: This variant (beta) lacks several exons in the coding region but maintains the reading frame, compared to variant alpha. It encodes isoform beta which is shorter than isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U20537.1, BM837197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTCGG CCTCGGGGCT CCGCAGGGGG CACCCGGCAG TGTCAACTGT TAGCCACGCA 
GATGCCGATT GCTTTGTGTG TGTCTTCCTG AGCCATGGCG AAGGCAATCA CATTTATGCA 
TATGATGCTA AAATCGAAAT TCAGACATTA ACTGGCTTGT TCAAAGGAGA CAAGTGTCAC 
AGCCTGGTTG GAAAACCCAA GATATTTATC ATTCAGGCAT GTCGGGGAAA CCAGCACGAT 
GTGCCAGTCA TTCCTTTGGA TGTAGTAGAT AATCAGACAG AGAAGTTGGA CACCAACATA 
ACTGAGGTGG ATGCAGCCTC CGTTTACACG CTGCCTGCTG GAGCTGACTT CCTCATGTGT 
TACTCTGTTG CAGAAGGATA TTATTCTCAC CGGGAAACTG TGAACGGCTC ATGGTACATT 
CAAGATTTGT GTGAGATGTT GGGAAAATAT GGCTCCTCCT TAGAGTTCAC AGAACTCCTC 
ACACTGGTGA ACAGGAAAGT TTCTCAGCGC CGAGTGGACT TTTGCAAAGA CCCAAGTGCA 
ATTGGAAAGA AGCAGGTTCC CTGTTTTGCC TCAATGCTAA CTAAAAAGCT GCATTTCTTT 
CCAAAATCTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116787.1
CDS	79..693
Translation	MSSASGLRRGHPAVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLHFFPKSN

Target ORF information:

RefSeq Version	NM_032992.2
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 6 (CASP6), transcript variant beta, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032992.2

ORF Insert Sequence:

ATGAGCTCGG CCTCGGGGCT CCGCAGGGGG CACCCGGCAG TGTCAACTGT TAGCCACGCA 
GATGCCGATT GCTTTGTGTG TGTCTTCCTG AGCCATGGCG AAGGCAATCA CATTTATGCA 
TATGATGCTA AAATCGAAAT TCAGACATTA ACTGGCTTGT TCAAAGGAGA CAAGTGTCAC 
AGCCTGGTTG GAAAACCCAA GATATTTATC ATTCAGGCAT GTCGGGGAAA CCAGCACGAT 
GTGCCAGTCA TTCCTTTGGA TGTAGTAGAT AATCAGACAG AGAAGTTGGA CACCAACATA 
ACTGAGGTGG ATGCAGCCTC CGTTTACACG CTGCCTGCTG GAGCTGACTT CCTCATGTGT 
TACTCTGTTG CAGAAGGATA TTATTCTCAC CGGGAAACTG TGAACGGCTC ATGGTACATT 
CAAGATTTGT GTGAGATGTT GGGAAAATAT GGCTCCTCCT TAGAGTTCAC AGAACTCCTC 
ACACTGGTGA ACAGGAAAGT TTCTCAGCGC CGAGTGGACT TTTGCAAAGA CCCAAGTGCA 
ATTGGAAAGA AGCAGGTTCC CTGTTTTGCC TCAATGCTAA CTAAAAAGCT GCATTTCTTT 
CCAAAATCTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiple proteolytic events in caspase-6 self-activation impact conformations of discrete structural regions.
Proceedings of the National Academy of Sciences of the United States of America114(38)E7977-E7986(2017 Sep)
Dagbay KB,Hardy JA

Tumor-Associated Mutations in Caspase-6 Negatively Impact Catalytic Efficiency.
Biochemistry56(34)4568-4577(2017 08)
Dagbay KB,Hill ME,Barrett E,Hardy JA

Ceramide phosphoethanolamine synthase SMSr is a target of caspase-6 during apoptotic cell death.
Bioscience reports37(4)(2017 Aug)
Cabukusta B,Nettebrock NT,Kol M,Hilderink A,Tafesse FG,Holthuis JCM

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CASP6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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