TAS1R3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TAS1R3
Entrez Gene ID	83756
Full Name	taste 1 receptor member 3
Synonyms	T1R3
General protein information	Preferred Names taste 1 receptor member 3 Names taste receptor type 1 member 3 sweet taste receptor T1R3 taste receptor, type 1, member 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.33
Summary	The protein encoded by this gene is a G-protein coupled receptor involved in taste responses. The encoded protein can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response. [provided by RefSeq, Nov 2015].
Disorder MIM:	605865

mRNA and Protein(s)

mRNA	Protein	Name
NM_152228.1	NP_689414.1	taste receptor type 1 member 3 precursor
NM_152228.3	NP_689414.2	taste receptor type 1 member 3 precursor
XM_017002435.1	XP_016857924.1	taste receptor type 1 member 3 isoform X1
XM_017002436.1	XP_016857925.1	taste receptor type 1 member 3 isoform X2
NM_152228.2	NP_689414.1	taste receptor type 1 member 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TAS1R3	XP_003307796.1
Gallus gallus (chicken)	TAS1R3	XP_425740.2
Danio rerio (zebrafish)	tas1r3	NP_001034717.1
Canis lupus familiaris (dog)	TAS1R3	NP_001026991.1
Mus musculus (house mouse)	Tas1r3	NP_114078.1
Rattus norvegicus (Norway rat)	Tas1r3	NP_570831.1
Homo sapiens (human)	TAS1R3	NP_689414.1
	TAS1R3	XP_001094084.2

Gene Ontology
Bibliography

Related articles in PubMed

Salivary leptin and TAS1R2/TAS1R3 polymorphisms are related to sweet taste sensitivity and carbohydrate intake from a buffet meal in healthy young adults.
Han P, Keast RSJ, Roura E
The British journal of nutrition118(10)763-770(2017 Nov)

The anatomy of mammalian sweet taste receptors.
Ch?ron JB, Golebiowski J, Antonczak S, Fiorucci S
Proteins85(2)332-341(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Regarding "consumption of carbohydrates (% energy) and higher amount of sweet foods, respectively...no associations were found for the TAS1R3 alleles."
Title: Salivary leptin and TAS1R2/TAS1R3 polymorphisms are related to sweet taste sensitivity and carbohydrate intake from a buffet meal in healthy young adults.

The molecular anatomy of sweet taste receptor dimers T1R2-T1R3 has been presented.
Title: The anatomy of mammalian sweet taste receptors.

T1R3 gene expression in the tongue is suppressed by chemotherapy.
Title: Effects of chemotherapy on gene expression of lingual taste receptors in patients with head and neck cancer.

The transcripts of TAS1R3 and UCN2 in peripheral blood cells may be considered potential biomarkers of consumption of sugary and fatty food, respectively, to complement data of food-intake questionnaires.
Title: TAS1R3 and UCN2 Transcript Levels in Blood Cells Are Associated With Sugary and Fatty Food Consumption in Children.

TAS1R3 gene rs307355 polymorphism found independent risk factor for dental caries experience by logistic regression & increased risk of caries. Moderate caries (4-7 caries) found to be associated w/TAS1R3 rs307355 heterozygous genotype
Title: Association of sweet taste receptor gene polymorphisms with dental caries experience in school children.

The following TAS1R3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TAS1R3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06691	NM_152228.2 Latest version!	Homo sapiens taste 1 receptor member 3 (TAS1R3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$429.50-$601.30 $859.00
OHu107275	NM_152228.3 Latest version!	Homo sapiens taste 1 receptor member 3 (TAS1R3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu90585	XM_017002435.1 Latest version!	Homo sapiens taste 1 receptor member 3 (TAS1R3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu90586	XM_017002436.1 Latest version!	Homo sapiens taste 1 receptor member 3 (TAS1R3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu06691	NM_152228.1	Homo sapiens taste receptor, type 1, member 3 (TAS1R3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$429.50-$601.30 $859.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TAS1R3 ( NM_152228.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_689414.1 Homo sapiens taste receptor, type 1, member 3 (TAS1R3), mRNA.

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CloneID	OHu06691
Clone ID Related Accession (Same CDS sequence)	NM_152228.1 , NM_152228.2
Accession Version	NM_152228.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	taste receptor type 1 member 3 precursor
Comment

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGA AGCCCGTGTC CCGGTGCTCG 
CGGCAGTGCC AGGAGGGCCA GGTGCGCCGG GTCAAGGGGT TCCACTCCTG CTGCTACGAC 
TGTGTGGACT GCGAGGCGGG CAGCTACCGG CAAAACCCAG ACGACATCGC CTGCACCTTT 
TGTGGCCAGG ATGAGTGGTC CCCGGAGCGA AGCACACGCT GCTTCCGCCG CAGGTCTCGG 
TTCCTGGCAT GGGGCGAGCC GGCTGTGCTG CTGCTGCTCC TGCTGCTGAG CCTGGCGCTG 
GGCCTTGTGC TGGCTGCTTT GGGGCTGTTC GTTCACCATC GGGACAGCCC ACTGGTTCAG 
GCCTCGGGGG GGCCCCTGGC CTGCTTTGGC CTGGTGTGCC TGGGCCTGGT CTGCCTCAGC 
GTCCTCCTGT TCCCTGGCCA GCCCAGCCCT GCCCGATGCC TGGCCCAGCA GCCCTTGTCC 
CACCTCCCGC TCACGGGCTG CCTGAGCACA CTCTTCCTGC AGGCGGCCGA GATCTTCGTG 
GAGTCAGAAC TGCCTCTGAG CTGGGCAGAC CGGCTGAGTG GCTGCCTGCG GGGGCCCTGG 
GCCTGGCTGG TGGTGCTGCT GGCCATGCTG GTGGAGGTCG CACTGTGCAC CTGGTACCTG 
GTGGCCTTCC CGCCGGAGGT GGTGACGGAC TGGCACATGC TGCCCACGGA GGCGCTGGTG 
CACTGCCGCA CACGCTCCTG GGTCAGCTTC GGCCTAGCGC ACGCCACCAA TGCCACGCTG 
GCCTTTCTCT GCTTCCTGGG CACTTTCCTG GTGCGGAGCC AGCCGGGCCG CTACAACCGT 
GCCCGTGGCC TCACCTTTGC CATGCTGGCC TACTTCATCA CCTGGGTCTC CTTTGTGCCC 
CTCCTGGCCA ATGTGCAGGT GGTCCTCAGG CCCGCCGTGC AGATGGGCGC CCTCCTGCTC 
TGTGTCCTGG GCATCCTGGC TGCCTTCCAC CTGCCCAGGT GTTACCTGCT CATGCGGCAG 
CCAGGGCTCA ACACCCCCGA GTTCTTCCTG GGAGGGGGCC CTGGGGATGC CCAAGGCCAG 
AATGACGGGA ACACAGGAAA TCAGGGGAAA CATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_689414.1

Translation

MLGPAVLGLSLWALLHPGTGAPLCLSQQLRMKGDYVLGGLFPLGEAEEAGLRSRTRPSSPVCTRFSSNGLLWALAMKMAVEEINNKSDLLPGLRLGYDLFDTCSEPVVAMKPSLMFLAKAGSRDIAAYCNYTQYQPRVLAVIGPHSSELAMVTGKFFSFFLMPQVSYGASMELLSARETFPSFFRTVPSDRVQLTAAAELLQEFGWNWVAALGSDDEYGRQGLSIFSALAAARGICIAHEGLVPLPRADDSRLGKVQDVLHQVNQSSVQVVLLFASVHAAHALFNYSISSRLSPKVWVASEAWLTSDLVMGLPGMAQMGTVLGFLQRGAQLHEFPQYVKTHLALATDPAFCSALGEREQGLEEDVVGQRCPQCDCITLQNVSAGLNHHQTFSVYAAVYSVAQALHNTLQCNASGCPAQDPVKPWQLLENMYNLTFHVGGLPLRFDSSGNVDMEYDLKLWVWQGSVPRLHDVGRFNGSLRTERLKIRWHTSDNQKPVSRCSRQCQEGQVRRVKGFHSCCYDCVDCEAGSYRQNPDDIACTFCGQDEWSPERSTRCFRRRSRFLAWGEPAVLLLLLLLSLALGLVLAALGLFVHHRDSPLVQASGGPLACFGLVCLGLVCLSVLLFPGQPSPARCLAQQPLSHLPLTGCLSTLFLQAAEIFVESELPLSWADRLSGCLRGPWAWLVVLLAMLVEVALCTWYLVAFPPEVVTDWHMLPTEALVHCRTRSWVSFGLAHATNATLAFLCFLGTFLVRSQPGRYNRARGLTFAMLAYFITWVSFVPLLANVQVVLRPAVQMGALLLCVLGILAAFHLPRCYLLMRQPGLNTPEFFLGGGPGDAQGQNDGNTGNQGKHE

Target ORF information:

RefSeq Version	NM_152228.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens taste receptor, type 1, member 3 (TAS1R3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152228.1

ORF Insert Sequence:

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGA AGCCCGTGTC CCGGTGCTCG 
CGGCAGTGCC AGGAGGGCCA GGTGCGCCGG GTCAAGGGGT TCCACTCCTG CTGCTACGAC 
TGTGTGGACT GCGAGGCGGG CAGCTACCGG CAAAACCCAG ACGACATCGC CTGCACCTTT 
TGTGGCCAGG ATGAGTGGTC CCCGGAGCGA AGCACACGCT GCTTCCGCCG CAGGTCTCGG 
TTCCTGGCAT GGGGCGAGCC GGCTGTGCTG CTGCTGCTCC TGCTGCTGAG CCTGGCGCTG 
GGCCTTGTGC TGGCTGCTTT GGGGCTGTTC GTTCACCATC GGGACAGCCC ACTGGTTCAG 
GCCTCGGGGG GGCCCCTGGC CTGCTTTGGC CTGGTGTGCC TGGGCCTGGT CTGCCTCAGC 
GTCCTCCTGT TCCCTGGCCA GCCCAGCCCT GCCCGATGCC TGGCCCAGCA GCCCTTGTCC 
CACCTCCCGC TCACGGGCTG CCTGAGCACA CTCTTCCTGC AGGCGGCCGA GATCTTCGTG 
GAGTCAGAAC TGCCTCTGAG CTGGGCAGAC CGGCTGAGTG GCTGCCTGCG GGGGCCCTGG 
GCCTGGCTGG TGGTGCTGCT GGCCATGCTG GTGGAGGTCG CACTGTGCAC CTGGTACCTG 
GTGGCCTTCC CGCCGGAGGT GGTGACGGAC TGGCACATGC TGCCCACGGA GGCGCTGGTG 
CACTGCCGCA CACGCTCCTG GGTCAGCTTC GGCCTAGCGC ACGCCACCAA TGCCACGCTG 
GCCTTTCTCT GCTTCCTGGG CACTTTCCTG GTGCGGAGCC AGCCGGGCCG CTACAACCGT 
GCCCGTGGCC TCACCTTTGC CATGCTGGCC TACTTCATCA CCTGGGTCTC CTTTGTGCCC 
CTCCTGGCCA ATGTGCAGGT GGTCCTCAGG CCCGCCGTGC AGATGGGCGC CCTCCTGCTC 
TGTGTCCTGG GCATCCTGGC TGCCTTCCAC CTGCCCAGGT GTTACCTGCT CATGCGGCAG 
CCAGGGCTCA ACACCCCCGA GTTCTTCCTG GGAGGGGGCC CTGGGGATGC CCAAGGCCAG 
AATGACGGGA ACACAGGAAA TCAGGGGAAA CATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R3 ( NM_152228.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689414.1 Homo sapiens taste 1 receptor member 3 (TAS1R3), mRNA.

Price & Availability↑

CloneID	OHu06691
Clone ID Related Accession (Same CDS sequence)	NM_152228.1 , NM_152228.2
Accession Version	NM_152228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BK000152.1, AL139287.24 and BU734688.1. On Nov 11, 2015 this sequence version replaced NM_152228.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK000152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGA AGCCCGTGTC CCGGTGCTCG 
CGGCAGTGCC AGGAGGGCCA GGTGCGCCGG GTCAAGGGGT TCCACTCCTG CTGCTACGAC 
TGTGTGGACT GCGAGGCGGG CAGCTACCGG CAAAACCCAG ACGACATCGC CTGCACCTTT 
TGTGGCCAGG ATGAGTGGTC CCCGGAGCGA AGCACACGCT GCTTCCGCCG CAGGTCTCGG 
TTCCTGGCAT GGGGCGAGCC GGCTGTGCTG CTGCTGCTCC TGCTGCTGAG CCTGGCGCTG 
GGCCTTGTGC TGGCTGCTTT GGGGCTGTTC GTTCACCATC GGGACAGCCC ACTGGTTCAG 
GCCTCGGGGG GGCCCCTGGC CTGCTTTGGC CTGGTGTGCC TGGGCCTGGT CTGCCTCAGC 
GTCCTCCTGT TCCCTGGCCA GCCCAGCCCT GCCCGATGCC TGGCCCAGCA GCCCTTGTCC 
CACCTCCCGC TCACGGGCTG CCTGAGCACA CTCTTCCTGC AGGCGGCCGA GATCTTCGTG 
GAGTCAGAAC TGCCTCTGAG CTGGGCAGAC CGGCTGAGTG GCTGCCTGCG GGGGCCCTGG 
GCCTGGCTGG TGGTGCTGCT GGCCATGCTG GTGGAGGTCG CACTGTGCAC CTGGTACCTG 
GTGGCCTTCC CGCCGGAGGT GGTGACGGAC TGGCACATGC TGCCCACGGA GGCGCTGGTG 
CACTGCCGCA CACGCTCCTG GGTCAGCTTC GGCCTAGCGC ACGCCACCAA TGCCACGCTG 
GCCTTTCTCT GCTTCCTGGG CACTTTCCTG GTGCGGAGCC AGCCGGGCCG CTACAACCGT 
GCCCGTGGCC TCACCTTTGC CATGCTGGCC TACTTCATCA CCTGGGTCTC CTTTGTGCCC 
CTCCTGGCCA ATGTGCAGGT GGTCCTCAGG CCCGCCGTGC AGATGGGCGC CCTCCTGCTC 
TGTGTCCTGG GCATCCTGGC TGCCTTCCAC CTGCCCAGGT GTTACCTGCT CATGCGGCAG 
CCAGGGCTCA ACACCCCCGA GTTCTTCCTG GGAGGGGGCC CTGGGGATGC CCAAGGCCAG 
AATGACGGGA ACACAGGAAA TCAGGGGAAA CATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689414.1
CDS	1..2559
Translation	MLGPAVLGLSLWALLHPGTGAPLCLSQQLRMKGDYVLGGLFPLGEAEEAGLRSRTRPSSPVCTRFSSNGLLWALAMKMAVEEINNKSDLLPGLRLGYDLFDTCSEPVVAMKPSLMFLAKAGSRDIAAYCNYTQYQPRVLAVIGPHSSELAMVTGKFFSFFLMPQVSYGASMELLSARETFPSFFRTVPSDRVQLTAAAELLQEFGWNWVAALGSDDEYGRQGLSIFSALAAARGICIAHEGLVPLPRADDSRLGKVQDVLHQVNQSSVQVVLLFASVHAAHALFNYSISSRLSPKVWVASEAWLTSDLVMGLPGMAQMGTVLGFLQRGAQLHEFPQYVKTHLALATDPAFCSALGEREQGLEEDVVGQRCPQCDCITLQNVSAGLNHHQTFSVYAAVYSVAQALHNTLQCNASGCPAQDPVKPWQLLENMYNLTFHVGGLPLRFDSSGNVDMEYDLKLWVWQGSVPRLHDVGRFNGSLRTERLKIRWHTSDNQKPVSRCSRQCQEGQVRRVKGFHSCCYDCVDCEAGSYRQNPDDIACTFCGQDEWSPERSTRCFRRRSRFLAWGEPAVLLLLLLLSLALGLVLAALGLFVHHRDSPLVQASGGPLACFGLVCLGLVCLSVLLFPGQPSPARCLAQQPLSHLPLTGCLSTLFLQAAEIFVESELPLSWADRLSGCLRGPWAWLVVLLAMLVEVALCTWYLVAFPPEVVTDWHMLPTEALVHCRTRSWVSFGLAHATNATLAFLCFLGTFLVRSQPGRYNRARGLTFAMLAYFITWVSFVPLLANVQVVLRPAVQMGALLLCVLGILAAFHLPRCYLLMRQPGLNTPEFFLGGGPGDAQGQNDGNTGNQGKHE

Target ORF information:

RefSeq Version	NM_152228.2
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 3 (TAS1R3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152228.2

ORF Insert Sequence:

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGA AGCCCGTGTC CCGGTGCTCG 
CGGCAGTGCC AGGAGGGCCA GGTGCGCCGG GTCAAGGGGT TCCACTCCTG CTGCTACGAC 
TGTGTGGACT GCGAGGCGGG CAGCTACCGG CAAAACCCAG ACGACATCGC CTGCACCTTT 
TGTGGCCAGG ATGAGTGGTC CCCGGAGCGA AGCACACGCT GCTTCCGCCG CAGGTCTCGG 
TTCCTGGCAT GGGGCGAGCC GGCTGTGCTG CTGCTGCTCC TGCTGCTGAG CCTGGCGCTG 
GGCCTTGTGC TGGCTGCTTT GGGGCTGTTC GTTCACCATC GGGACAGCCC ACTGGTTCAG 
GCCTCGGGGG GGCCCCTGGC CTGCTTTGGC CTGGTGTGCC TGGGCCTGGT CTGCCTCAGC 
GTCCTCCTGT TCCCTGGCCA GCCCAGCCCT GCCCGATGCC TGGCCCAGCA GCCCTTGTCC 
CACCTCCCGC TCACGGGCTG CCTGAGCACA CTCTTCCTGC AGGCGGCCGA GATCTTCGTG 
GAGTCAGAAC TGCCTCTGAG CTGGGCAGAC CGGCTGAGTG GCTGCCTGCG GGGGCCCTGG 
GCCTGGCTGG TGGTGCTGCT GGCCATGCTG GTGGAGGTCG CACTGTGCAC CTGGTACCTG 
GTGGCCTTCC CGCCGGAGGT GGTGACGGAC TGGCACATGC TGCCCACGGA GGCGCTGGTG 
CACTGCCGCA CACGCTCCTG GGTCAGCTTC GGCCTAGCGC ACGCCACCAA TGCCACGCTG 
GCCTTTCTCT GCTTCCTGGG CACTTTCCTG GTGCGGAGCC AGCCGGGCCG CTACAACCGT 
GCCCGTGGCC TCACCTTTGC CATGCTGGCC TACTTCATCA CCTGGGTCTC CTTTGTGCCC 
CTCCTGGCCA ATGTGCAGGT GGTCCTCAGG CCCGCCGTGC AGATGGGCGC CCTCCTGCTC 
TGTGTCCTGG GCATCCTGGC TGCCTTCCAC CTGCCCAGGT GTTACCTGCT CATGCGGCAG 
CCAGGGCTCA ACACCCCCGA GTTCTTCCTG GGAGGGGGCC CTGGGGATGC CCAAGGCCAG 
AATGACGGGA ACACAGGAAA TCAGGGGAAA CATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R3 ( NM_152228.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689414.2 Homo sapiens taste 1 receptor member 3 (TAS1R3), mRNA.

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CloneID	OHu107275
Clone ID Related Accession (Same CDS sequence)	NM_152228.3
Accession Version	NM_152228.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139287.24. On Nov 23, 2018 this sequence version replaced NM_152228.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK000152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000339381.6/ ENSP00000344411.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGA AGCCCGTGTC CCGGTGCTCG 
CGGCAGTGCC AGGAGGGCCA GGTGCGCCGG GTCAAGGGGT TCCACTCCTG CTGCTACGAC 
TGTGTGGACT GCGAGGCGGG CAGCTACCGG CAAAACCCAG ACGACATCGC CTGCACCTTT 
TGTGGCCAGG ATGAGTGGTC CCCGGAGCGA AGCACACGCT GCTTCCGCCG CAGGTCTCGG 
TTCCTGGCAT GGGGCGAGCC GGCTGTGCTG CTGCTGCTCC TGCTGCTGAG CCTGGCGCTG 
GGCCTTGTGC TGGCTGCTTT GGGGCTGTTC GTTCACCATC GGGACAGCCC ACTGGTTCAG 
GCCTCGGGGG GGCCCCTGGC CTGCTTTGGC CTGGTGTGCC TGGGCCTGGT CTGCCTCAGC 
GTCCTCCTGT TCCCTGGCCA GCCCAGCCCT GCCCGATGCC TGGCCCAGCA GCCCTTGTCC 
CACCTCCCGC TCACGGGCTG CCTGAGCACA CTCTTCCTGC AGGCGGCCGA GATCTTCGTG 
GAGTCAGAAC TGCCTCTGAG CTGGGCAGAC CGGCTGAGTG GCTGCCTGCG GGGGCCCTGG 
GCCTGGCTGG TGGTGCTGCT GGCCATGCTG GTGGAGGTCG CACTGTGCAC CTGGTACCTG 
GTGGCCTTCC CGCCGGAGGT GGTGACGGAC TGGCACATGC TGCCCACGGA GGCGCTGGTG 
CACTGCCGCA CACGCTCCTG GGTCAGCTTC GGCCTAGCGC ACGCCACCAA TGCCACGCTG 
GCCTTTCTCT GCTTCCTGGG CACTTTCCTG GTGCGGAGCC AGCCGGGCTG CTACAACCGT 
GCCCGTGGCC TCACCTTTGC CATGCTGGCC TACTTCATCA CCTGGGTCTC CTTTGTGCCC 
CTCCTGGCCA ATGTGCAGGT GGTCCTCAGG CCCGCCGTGC AGATGGGCGC CCTCCTGCTC 
TGTGTCCTGG GCATCCTGGC TGCCTTCCAC CTGCCCAGGT GTTACCTGCT CATGCGGCAG 
CCAGGGCTCA ACACCCCCGA GTTCTTCCTG GGAGGGGGCC CTGGGGATGC CCAAGGCCAG 
AATGACGGGA ACACAGGAAA TCAGGGGAAA CATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689414.2
CDS	67..2625
Translation	MLGPAVLGLSLWALLHPGTGAPLCLSQQLRMKGDYVLGGLFPLGEAEEAGLRSRTRPSSPVCTRFSSNGLLWALAMKMAVEEINNKSDLLPGLRLGYDLFDTCSEPVVAMKPSLMFLAKAGSRDIAAYCNYTQYQPRVLAVIGPHSSELAMVTGKFFSFFLMPQVSYGASMELLSARETFPSFFRTVPSDRVQLTAAAELLQEFGWNWVAALGSDDEYGRQGLSIFSALAAARGICIAHEGLVPLPRADDSRLGKVQDVLHQVNQSSVQVVLLFASVHAAHALFNYSISSRLSPKVWVASEAWLTSDLVMGLPGMAQMGTVLGFLQRGAQLHEFPQYVKTHLALATDPAFCSALGEREQGLEEDVVGQRCPQCDCITLQNVSAGLNHHQTFSVYAAVYSVAQALHNTLQCNASGCPAQDPVKPWQLLENMYNLTFHVGGLPLRFDSSGNVDMEYDLKLWVWQGSVPRLHDVGRFNGSLRTERLKIRWHTSDNQKPVSRCSRQCQEGQVRRVKGFHSCCYDCVDCEAGSYRQNPDDIACTFCGQDEWSPERSTRCFRRRSRFLAWGEPAVLLLLLLLSLALGLVLAALGLFVHHRDSPLVQASGGPLACFGLVCLGLVCLSVLLFPGQPSPARCLAQQPLSHLPLTGCLSTLFLQAAEIFVESELPLSWADRLSGCLRGPWAWLVVLLAMLVEVALCTWYLVAFPPEVVTDWHMLPTEALVHCRTRSWVSFGLAHATNATLAFLCFLGTFLVRSQPGCYNRARGLTFAMLAYFITWVSFVPLLANVQVVLRPAVQMGALLLCVLGILAAFHLPRCYLLMRQPGLNTPEFFLGGGPGDAQGQNDGNTGNQGKHE

Target ORF information:

RefSeq Version	NM_152228.3
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 3 (TAS1R3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152228.3

ORF Insert Sequence:

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGA AGCCCGTGTC CCGGTGCTCG 
CGGCAGTGCC AGGAGGGCCA GGTGCGCCGG GTCAAGGGGT TCCACTCCTG CTGCTACGAC 
TGTGTGGACT GCGAGGCGGG CAGCTACCGG CAAAACCCAG ACGACATCGC CTGCACCTTT 
TGTGGCCAGG ATGAGTGGTC CCCGGAGCGA AGCACACGCT GCTTCCGCCG CAGGTCTCGG 
TTCCTGGCAT GGGGCGAGCC GGCTGTGCTG CTGCTGCTCC TGCTGCTGAG CCTGGCGCTG 
GGCCTTGTGC TGGCTGCTTT GGGGCTGTTC GTTCACCATC GGGACAGCCC ACTGGTTCAG 
GCCTCGGGGG GGCCCCTGGC CTGCTTTGGC CTGGTGTGCC TGGGCCTGGT CTGCCTCAGC 
GTCCTCCTGT TCCCTGGCCA GCCCAGCCCT GCCCGATGCC TGGCCCAGCA GCCCTTGTCC 
CACCTCCCGC TCACGGGCTG CCTGAGCACA CTCTTCCTGC AGGCGGCCGA GATCTTCGTG 
GAGTCAGAAC TGCCTCTGAG CTGGGCAGAC CGGCTGAGTG GCTGCCTGCG GGGGCCCTGG 
GCCTGGCTGG TGGTGCTGCT GGCCATGCTG GTGGAGGTCG CACTGTGCAC CTGGTACCTG 
GTGGCCTTCC CGCCGGAGGT GGTGACGGAC TGGCACATGC TGCCCACGGA GGCGCTGGTG 
CACTGCCGCA CACGCTCCTG GGTCAGCTTC GGCCTAGCGC ACGCCACCAA TGCCACGCTG 
GCCTTTCTCT GCTTCCTGGG CACTTTCCTG GTGCGGAGCC AGCCGGGCTG CTACAACCGT 
GCCCGTGGCC TCACCTTTGC CATGCTGGCC TACTTCATCA CCTGGGTCTC CTTTGTGCCC 
CTCCTGGCCA ATGTGCAGGT GGTCCTCAGG CCCGCCGTGC AGATGGGCGC CCTCCTGCTC 
TGTGTCCTGG GCATCCTGGC TGCCTTCCAC CTGCCCAGGT GTTACCTGCT CATGCGGCAG 
CCAGGGCTCA ACACCCCCGA GTTCTTCCTG GGAGGGGGCC CTGGGGATGC CCAAGGCCAG 
AATGACGGGA ACACAGGAAA TCAGGGGAAA CATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R3 ( XM_017002435.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857924.1 Homo sapiens taste 1 receptor member 3 (TAS1R3), transcript variant X1, mRNA.

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CloneID	OHu90585
Clone ID Related Accession (Same CDS sequence)	XM_017002435.1
Accession Version	XM_017002435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGA AGCCCGTGTC CCGGTGCTCG 
CGGCAGTGCC AGGAGGGCCA GGTGCGCCGG GTCAAGGGGT TCCACTCCTG CTGCTACGAC 
TGTGTGGACT GCGAGGCGGG CAGCTACCGG CAAAACCCAG GTGAGCCGCC TTCCCGGCAG 
GCGGGGGTGG GAACGCAGCA GGGGAGGGTC CTGCCAAGTC CTGACTCTGA GACCAGAGCC 
CACAGGGTAC AAGACGAACA CCCAGCGCCC TTCTCCTCTC TCACAGACGA CATCGCCTGC 
ACCTTTTGTG GCCAGGATGA GTGGTCCCCG GAGCGAAGCA CACGCTGCTT CCGCCGCAGG 
TCTCGGTTCC TGGCATGGGG CGAGCCGGCT GTGCTGCTGC TGCTCCTGCT GCTGAGCCTG 
GCGCTGGGCC TTGTGCTGGC TGCTTTGGGG CTGTTCGTTC ACCATCGGGA CAGCCCACTG 
GTTCAGGCCT CGGGGGGGCC CCTGGCCTGC TTTGGCCTGG TGTGCCTGGG CCTGGTCTGC 
CTCAGCGTCC TCCTGTTCCC TGGCCAGCCC AGCCCTGCCC GATGCCTGGC CCAGCAGCCC 
TTGTCCCACC TCCCGCTCAC GGGCTGCCTG AGCACACTCT TCCTGCAGGC GGCCGAGATC 
TTCGTGGAGT CAGAACTGCC TCTGAGCTGG GCAGACCGGC TGAGTGGCTG CCTGCGGGGG 
CCCTGGGCCT GGCTGGTGGT GCTGCTGGCC ATGCTGGTGG AGGTCGCACT GTGCACCTGG 
TACCTGGTGG CCTTCCCGCC GGAGGTGGTG ACGGACTGGC ACATGCTGCC CACGGAGGCG 
CTGGTGCACT GCCGCACACG CTCCTGGGTC AGCTTCGGCC TAGCGCACGC CACCAATGCC 
ACGCTGGCCT TTCTCTGCTT CCTGGGCACT TTCCTGGTGC GGAGCCAGCC GGGCTGCTAC 
AACCGTGCCC GTGGCCTCAC CTTTGCCATG CTGGCCTACT TCATCACCTG GGTCTCCTTT 
GTGCCCCTCC TGGCCAATGT GCAGGTGGTC CTCAGGCCCG CCGTGCAGAT GGGCGCCCTC 
CTGCTCTGTG TCCTGGGCAT CCTGGCTGCC TTCCACCTGC CCAGGTGTTA CCTGCTCATG 
CGGCAGCCAG GGCTCAACAC CCCCGAGTTC TTCCTGGGAG GGGGCCCTGG GGATGCCCAA 
GGCCAGAATG ACGGGAACAC AGGAAATCAG GGGAAACATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857924.1
CDS	23..2707
Translation	MLGPAVLGLSLWALLHPGTGAPLCLSQQLRMKGDYVLGGLFPLGEAEEAGLRSRTRPSSPVCTRFSSNGLLWALAMKMAVEEINNKSDLLPGLRLGYDLFDTCSEPVVAMKPSLMFLAKAGSRDIAAYCNYTQYQPRVLAVIGPHSSELAMVTGKFFSFFLMPQVSYGASMELLSARETFPSFFRTVPSDRVQLTAAAELLQEFGWNWVAALGSDDEYGRQGLSIFSALAAARGICIAHEGLVPLPRADDSRLGKVQDVLHQVNQSSVQVVLLFASVHAAHALFNYSISSRLSPKVWVASEAWLTSDLVMGLPGMAQMGTVLGFLQRGAQLHEFPQYVKTHLALATDPAFCSALGEREQGLEEDVVGQRCPQCDCITLQNVSAGLNHHQTFSVYAAVYSVAQALHNTLQCNASGCPAQDPVKPWQLLENMYNLTFHVGGLPLRFDSSGNVDMEYDLKLWVWQGSVPRLHDVGRFNGSLRTERLKIRWHTSDNQKPVSRCSRQCQEGQVRRVKGFHSCCYDCVDCEAGSYRQNPGEPPSRQAGVGTQQGRVLPSPDSETRAHRVQDEHPAPFSSLTDDIACTFCGQDEWSPERSTRCFRRRSRFLAWGEPAVLLLLLLLSLALGLVLAALGLFVHHRDSPLVQASGGPLACFGLVCLGLVCLSVLLFPGQPSPARCLAQQPLSHLPLTGCLSTLFLQAAEIFVESELPLSWADRLSGCLRGPWAWLVVLLAMLVEVALCTWYLVAFPPEVVTDWHMLPTEALVHCRTRSWVSFGLAHATNATLAFLCFLGTFLVRSQPGCYNRARGLTFAMLAYFITWVSFVPLLANVQVVLRPAVQMGALLLCVLGILAAFHLPRCYLLMRQPGLNTPEFFLGGGPGDAQGQNDGNTGNQGKHE

Target ORF information:

RefSeq Version	XM_017002435.1
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 3 (TAS1R3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002435.1

ORF Insert Sequence:

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGA AGCCCGTGTC CCGGTGCTCG 
CGGCAGTGCC AGGAGGGCCA GGTGCGCCGG GTCAAGGGGT TCCACTCCTG CTGCTACGAC 
TGTGTGGACT GCGAGGCGGG CAGCTACCGG CAAAACCCAG GTGAGCCGCC TTCCCGGCAG 
GCGGGGGTGG GAACGCAGCA GGGGAGGGTC CTGCCAAGTC CTGACTCTGA GACCAGAGCC 
CACAGGGTAC AAGACGAACA CCCAGCGCCC TTCTCCTCTC TCACAGACGA CATCGCCTGC 
ACCTTTTGTG GCCAGGATGA GTGGTCCCCG GAGCGAAGCA CACGCTGCTT CCGCCGCAGG 
TCTCGGTTCC TGGCATGGGG CGAGCCGGCT GTGCTGCTGC TGCTCCTGCT GCTGAGCCTG 
GCGCTGGGCC TTGTGCTGGC TGCTTTGGGG CTGTTCGTTC ACCATCGGGA CAGCCCACTG 
GTTCAGGCCT CGGGGGGGCC CCTGGCCTGC TTTGGCCTGG TGTGCCTGGG CCTGGTCTGC 
CTCAGCGTCC TCCTGTTCCC TGGCCAGCCC AGCCCTGCCC GATGCCTGGC CCAGCAGCCC 
TTGTCCCACC TCCCGCTCAC GGGCTGCCTG AGCACACTCT TCCTGCAGGC GGCCGAGATC 
TTCGTGGAGT CAGAACTGCC TCTGAGCTGG GCAGACCGGC TGAGTGGCTG CCTGCGGGGG 
CCCTGGGCCT GGCTGGTGGT GCTGCTGGCC ATGCTGGTGG AGGTCGCACT GTGCACCTGG 
TACCTGGTGG CCTTCCCGCC GGAGGTGGTG ACGGACTGGC ACATGCTGCC CACGGAGGCG 
CTGGTGCACT GCCGCACACG CTCCTGGGTC AGCTTCGGCC TAGCGCACGC CACCAATGCC 
ACGCTGGCCT TTCTCTGCTT CCTGGGCACT TTCCTGGTGC GGAGCCAGCC GGGCTGCTAC 
AACCGTGCCC GTGGCCTCAC CTTTGCCATG CTGGCCTACT TCATCACCTG GGTCTCCTTT 
GTGCCCCTCC TGGCCAATGT GCAGGTGGTC CTCAGGCCCG CCGTGCAGAT GGGCGCCCTC 
CTGCTCTGTG TCCTGGGCAT CCTGGCTGCC TTCCACCTGC CCAGGTGTTA CCTGCTCATG 
CGGCAGCCAG GGCTCAACAC CCCCGAGTTC TTCCTGGGAG GGGGCCCTGG GGATGCCCAA 
GGCCAGAATG ACGGGAACAC AGGAAATCAG GGGAAACATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R3 ( XM_017002436.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857925.1 Homo sapiens taste 1 receptor member 3 (TAS1R3), transcript variant X2, mRNA.

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CloneID	OHu90586
Clone ID Related Accession (Same CDS sequence)	XM_017002436.1
Accession Version	XM_017002436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGC CCGTGTCCCG GTGCTCGCGG 
CAGTGCCAGG AGGGCCAGGT GCGCCGGGTC AAGGGGTTCC ACTCCTGCTG CTACGACTGT 
GTGGACTGCG AGGCGGGCAG CTACCGGCAA AACCCAGGTG AGCCGCCTTC CCGGCAGGCG 
GGGGTGGGAA CGCAGCAGGG GAGGGTCCTG CCAAGTCCTG ACTCTGAGAC CAGAGCCCAC 
AGGGTACAAG ACGAACACCC AGCGCCCTTC TCCTCTCTCA CAGACGACAT CGCCTGCACC 
TTTTGTGGCC AGGATGAGTG GTCCCCGGAG CGAAGCACAC GCTGCTTCCG CCGCAGGTCT 
CGGTTCCTGG CATGGGGCGA GCCGGCTGTG CTGCTGCTGC TCCTGCTGCT GAGCCTGGCG 
CTGGGCCTTG TGCTGGCTGC TTTGGGGCTG TTCGTTCACC ATCGGGACAG CCCACTGGTT 
CAGGCCTCGG GGGGGCCCCT GGCCTGCTTT GGCCTGGTGT GCCTGGGCCT GGTCTGCCTC 
AGCGTCCTCC TGTTCCCTGG CCAGCCCAGC CCTGCCCGAT GCCTGGCCCA GCAGCCCTTG 
TCCCACCTCC CGCTCACGGG CTGCCTGAGC ACACTCTTCC TGCAGGCGGC CGAGATCTTC 
GTGGAGTCAG AACTGCCTCT GAGCTGGGCA GACCGGCTGA GTGGCTGCCT GCGGGGGCCC 
TGGGCCTGGC TGGTGGTGCT GCTGGCCATG CTGGTGGAGG TCGCACTGTG CACCTGGTAC 
CTGGTGGCCT TCCCGCCGGA GGTGGTGACG GACTGGCACA TGCTGCCCAC GGAGGCGCTG 
GTGCACTGCC GCACACGCTC CTGGGTCAGC TTCGGCCTAG CGCACGCCAC CAATGCCACG 
CTGGCCTTTC TCTGCTTCCT GGGCACTTTC CTGGTGCGGA GCCAGCCGGG CTGCTACAAC 
CGTGCCCGTG GCCTCACCTT TGCCATGCTG GCCTACTTCA TCACCTGGGT CTCCTTTGTG 
CCCCTCCTGG CCAATGTGCA GGTGGTCCTC AGGCCCGCCG TGCAGATGGG CGCCCTCCTG 
CTCTGTGTCC TGGGCATCCT GGCTGCCTTC CACCTGCCCA GGTGTTACCT GCTCATGCGG 
CAGCCAGGGC TCAACACCCC CGAGTTCTTC CTGGGAGGGG GCCCTGGGGA TGCCCAAGGC 
CAGAATGACG GGAACACAGG AAATCAGGGG AAACATGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857925.1
CDS	23..2704
Translation	MLGPAVLGLSLWALLHPGTGAPLCLSQQLRMKGDYVLGGLFPLGEAEEAGLRSRTRPSSPVCTRFSSNGLLWALAMKMAVEEINNKSDLLPGLRLGYDLFDTCSEPVVAMKPSLMFLAKAGSRDIAAYCNYTQYQPRVLAVIGPHSSELAMVTGKFFSFFLMPQVSYGASMELLSARETFPSFFRTVPSDRVQLTAAAELLQEFGWNWVAALGSDDEYGRQGLSIFSALAAARGICIAHEGLVPLPRADDSRLGKVQDVLHQVNQSSVQVVLLFASVHAAHALFNYSISSRLSPKVWVASEAWLTSDLVMGLPGMAQMGTVLGFLQRGAQLHEFPQYVKTHLALATDPAFCSALGEREQGLEEDVVGQRCPQCDCITLQNVSAGLNHHQTFSVYAAVYSVAQALHNTLQCNASGCPAQDPVKPWQLLENMYNLTFHVGGLPLRFDSSGNVDMEYDLKLWVWQGSVPRLHDVGRFNGSLRTERLKIRWHTSDNQPVSRCSRQCQEGQVRRVKGFHSCCYDCVDCEAGSYRQNPGEPPSRQAGVGTQQGRVLPSPDSETRAHRVQDEHPAPFSSLTDDIACTFCGQDEWSPERSTRCFRRRSRFLAWGEPAVLLLLLLLSLALGLVLAALGLFVHHRDSPLVQASGGPLACFGLVCLGLVCLSVLLFPGQPSPARCLAQQPLSHLPLTGCLSTLFLQAAEIFVESELPLSWADRLSGCLRGPWAWLVVLLAMLVEVALCTWYLVAFPPEVVTDWHMLPTEALVHCRTRSWVSFGLAHATNATLAFLCFLGTFLVRSQPGCYNRARGLTFAMLAYFITWVSFVPLLANVQVVLRPAVQMGALLLCVLGILAAFHLPRCYLLMRQPGLNTPEFFLGGGPGDAQGQNDGNTGNQGKHE

Target ORF information:

RefSeq Version	XM_017002436.1
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 3 (TAS1R3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002436.1

ORF Insert Sequence:

ATGCTGGGCC CTGCTGTCCT GGGCCTCAGC CTCTGGGCTC TCCTGCACCC TGGGACGGGG 
GCCCCATTGT GCCTGTCACA GCAACTTAGG ATGAAGGGGG ACTACGTGCT GGGGGGGCTG 
TTCCCCCTGG GCGAGGCCGA GGAGGCTGGC CTCCGCAGCC GGACACGGCC CAGCAGCCCT 
GTGTGCACCA GGTTCTCCTC AAACGGCCTG CTCTGGGCAC TGGCCATGAA AATGGCCGTG 
GAGGAGATCA ACAACAAGTC GGATCTGCTG CCCGGGCTGC GCCTGGGCTA CGACCTCTTT 
GATACGTGCT CGGAGCCTGT GGTGGCCATG AAGCCCAGCC TCATGTTCCT GGCCAAGGCA 
GGCAGCCGCG ACATCGCCGC CTACTGCAAC TACACGCAGT ACCAGCCCCG TGTGCTGGCT 
GTCATCGGGC CCCACTCGTC AGAGCTCGCC ATGGTCACCG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCCC AGGTCAGCTA CGGTGCTAGC ATGGAGCTGC TGAGCGCCCG GGAGACCTTC 
CCCTCCTTCT TCCGCACCGT GCCCAGCGAC CGTGTGCAGC TGACGGCCGC CGCGGAGCTG 
CTGCAGGAGT TCGGCTGGAA CTGGGTGGCC GCCCTGGGCA GCGACGACGA GTACGGCCGG 
CAGGGCCTGA GCATCTTCTC GGCCCTGGCC GCGGCACGCG GCATCTGCAT CGCGCACGAG 
GGCCTGGTGC CGCTGCCCCG TGCCGATGAC TCGCGGCTGG GGAAGGTGCA GGACGTCCTG 
CACCAGGTGA ACCAGAGCAG CGTGCAGGTG GTGCTGCTGT TCGCCTCCGT GCACGCCGCC 
CACGCCCTCT TCAACTACAG CATCAGCAGC AGGCTCTCGC CCAAGGTGTG GGTGGCCAGC 
GAGGCCTGGC TGACCTCTGA CCTGGTCATG GGGCTGCCCG GCATGGCCCA GATGGGCACG 
GTGCTTGGCT TCCTCCAGAG GGGTGCCCAG CTGCACGAGT TCCCCCAGTA CGTGAAGACG 
CACCTGGCCC TGGCCACCGA CCCGGCCTTC TGCTCTGCCC TGGGCGAGAG GGAGCAGGGT 
CTGGAGGAGG ACGTGGTGGG CCAGCGCTGC CCGCAGTGTG ACTGCATCAC GCTGCAGAAC 
GTGAGCGCAG GGCTAAATCA CCACCAGACG TTCTCTGTCT ACGCAGCTGT GTATAGCGTG 
GCCCAGGCCC TGCACAACAC TCTTCAGTGC AACGCCTCAG GCTGCCCCGC GCAGGACCCC 
GTGAAGCCCT GGCAGCTCCT GGAGAACATG TACAACCTGA CCTTCCACGT GGGCGGGCTG 
CCGCTGCGGT TCGACAGCAG CGGAAACGTG GACATGGAGT ACGACCTGAA GCTGTGGGTG 
TGGCAGGGCT CAGTGCCCAG GCTCCACGAC GTGGGCAGGT TCAACGGCAG CCTCAGGACA 
GAGCGCCTGA AGATCCGCTG GCACACGTCT GACAACCAGC CCGTGTCCCG GTGCTCGCGG 
CAGTGCCAGG AGGGCCAGGT GCGCCGGGTC AAGGGGTTCC ACTCCTGCTG CTACGACTGT 
GTGGACTGCG AGGCGGGCAG CTACCGGCAA AACCCAGGTG AGCCGCCTTC CCGGCAGGCG 
GGGGTGGGAA CGCAGCAGGG GAGGGTCCTG CCAAGTCCTG ACTCTGAGAC CAGAGCCCAC 
AGGGTACAAG ACGAACACCC AGCGCCCTTC TCCTCTCTCA CAGACGACAT CGCCTGCACC 
TTTTGTGGCC AGGATGAGTG GTCCCCGGAG CGAAGCACAC GCTGCTTCCG CCGCAGGTCT 
CGGTTCCTGG CATGGGGCGA GCCGGCTGTG CTGCTGCTGC TCCTGCTGCT GAGCCTGGCG 
CTGGGCCTTG TGCTGGCTGC TTTGGGGCTG TTCGTTCACC ATCGGGACAG CCCACTGGTT 
CAGGCCTCGG GGGGGCCCCT GGCCTGCTTT GGCCTGGTGT GCCTGGGCCT GGTCTGCCTC 
AGCGTCCTCC TGTTCCCTGG CCAGCCCAGC CCTGCCCGAT GCCTGGCCCA GCAGCCCTTG 
TCCCACCTCC CGCTCACGGG CTGCCTGAGC ACACTCTTCC TGCAGGCGGC CGAGATCTTC 
GTGGAGTCAG AACTGCCTCT GAGCTGGGCA GACCGGCTGA GTGGCTGCCT GCGGGGGCCC 
TGGGCCTGGC TGGTGGTGCT GCTGGCCATG CTGGTGGAGG TCGCACTGTG CACCTGGTAC 
CTGGTGGCCT TCCCGCCGGA GGTGGTGACG GACTGGCACA TGCTGCCCAC GGAGGCGCTG 
GTGCACTGCC GCACACGCTC CTGGGTCAGC TTCGGCCTAG CGCACGCCAC CAATGCCACG 
CTGGCCTTTC TCTGCTTCCT GGGCACTTTC CTGGTGCGGA GCCAGCCGGG CTGCTACAAC 
CGTGCCCGTG GCCTCACCTT TGCCATGCTG GCCTACTTCA TCACCTGGGT CTCCTTTGTG 
CCCCTCCTGG CCAATGTGCA GGTGGTCCTC AGGCCCGCCG TGCAGATGGG CGCCCTCCTG 
CTCTGTGTCC TGGGCATCCT GGCTGCCTTC CACCTGCCCA GGTGTTACCT GCTCATGCGG 
CAGCCAGGGC TCAACACCCC CGAGTTCTTC CTGGGAGGGG GCCCTGGGGA TGCCCAAGGC 
CAGAATGACG GGAACACAGG AAATCAGGGG AAACATGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Salivary leptin and TAS1R2/TAS1R3 polymorphisms are related to sweet taste sensitivity and carbohydrate intake from a buffet meal in healthy young adults.
The British journal of nutrition118(10)763-770(2017 Nov)
Han P,Keast RSJ,Roura E

The anatomy of mammalian sweet taste receptors.
Proteins85(2)332-341(2017 Feb)
Ch?ron JB,Golebiowski J,Antonczak S,Fiorucci S

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TAS1R3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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