GRWD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRWD1
Entrez Gene ID	83743
Full Name	glutamate rich WD repeat containing 1
Synonyms	CDW4,GRWD,RRB1,WDR28
General protein information	Preferred Names glutamate rich WD repeat containing 1 Names glutamate-rich WD repeat-containing protein 1 CUL4- and DDB1-associated WDR protein 4 WD repeat domain 28 glutamate rich WD repeat protein GRWD regulator of ribosome biogenesis 1 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	This gene encodes a glutamate-rich protein that contains five WD-repeat motifs. The encoded protein may play a critical role in ribosome biogenesis and may also play a role in histone methylation through interactions with CUL4-DDB1 ubiquitin E3 ligase. [provided by RefSeq, Feb 2012].
Disorder MIM:	610597

mRNA and Protein(s)

mRNA	Protein	Name
NM_031485.3	NP_113673.3	glutamate-rich WD repeat-containing protein 1
NM_031485.4	NP_113673.3	glutamate-rich WD repeat-containing protein 1

Pathways from BioSystems

Homologies

	GRWD1	XP_001113650.1
Bos taurus (cattle)	GRWD1	NP_001069707.1
Kluyveromyces lactis	KLLA0F17237g	XP_455855.1
Arabidopsis thaliana (thale cress)	AT2G19540	NP_179544.1
Danio rerio (zebrafish)	grwd1	NP_001003509.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007125	XP_308636.2
Eremothecium gossypii	AGOS_ADR242C	NP_984338.2
Oryza sativa (rice)	Os11g0134500	NP_001065677.1
Xenopus tropicalis (tropical clawed frog)	grwd1	NP_989268.3
Pan troglodytes (chimpanzee)	GRWD1	XP_003316533.1
Canis lupus familiaris (dog)	GRWD1	XP_854878.1
Mus musculus (house mouse)	Grwd1	NP_700468.2
Drosophila melanogaster (fruit fly)	l(2)09851	NP_001260713.1
Neurospora crassa	NCU09521	XP_958516.1
Rattus norvegicus (Norway rat)	Grwd1	NP_001012067.1
Caenorhabditis elegans (roundworm)	CELE_Y54H5A.1	NP_498091.1
Magnaporthe oryzae (rice blast fungus)	MGG_14350	XP_003717750.1
Homo sapiens (human)	GRWD1	NP_113673.3
Saccharomyces cerevisiae (baker's yeast)	RRB1	NP_013850.1
Schizosaccharomyces pombe (fission yeast)	SPBC1711.07	NP_595880.1

Gene Ontology
Bibliography

Related articles in PubMed

GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis.
Kayama K, Watanabe S, Takafuji T, Tsuji T, Hironaka K, Matsumoto M, Nakayama KI, Enari M, Kohno T, Shiraishi K, Kiyono T, Yoshida K, Sugimoto N, Fujita M
EMBO reports18(1)123-137(2017 01)

Nucleosome assembly and disassembly activity of GRWD1, a novel Cdt1-binding protein that promotes pre-replication complex formation.
Aizawa M, Sugimoto N, Watanabe S, Yoshida K, Fujita M
Biochimica et biophysica acta1863(11)2739-2748(2016 11)

Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture.
Sugimoto N, Maehara K, Yoshida K, Yasukouchi S, Osano S, Watanabe S, Aizawa M, Yugawa T, Kiyono T, Kurumizaka H, Ohkawa Y, Fujita M
Nucleic acids research43(12)5898-911(2015 Jul)

A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
Cloutier P, Lavall?e-Adam M, Faubert D, Blanchette M, Coulombe B
PLoS genetics9(1)e1003210(2013)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GRWD1 overexpression in combination with HPV16 E7 and activated KRAS confers anchorage-independent growth and tumorigenic capacity on normal human fibroblasts. Consistent with this, GRWD1 overexpression is associated with poor prognosis in cancer patients.
Title: GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis.

Cdt1-binding protein GRWD1 promotes chromatin fluidity by influencing nucleosome structures, e.g., by transient eviction of H2A-H2B, and thereby promotes efficient MCM loading at replication origins.
Title: Nucleosome assembly and disassembly activity of GRWD1, a novel Cdt1-binding protein that promotes pre-replication complex formation.

The following GRWD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRWD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31530	NM_031485.4 Latest version!	Homo sapiens glutamate rich WD repeat containing 1 (GRWD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu31530	NM_031485.3	Homo sapiens glutamate rich WD repeat containing 1 (GRWD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRWD1 ( NM_031485.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_113673.3 Homo sapiens glutamate rich WD repeat containing 1 (GRWD1), mRNA.

Price & Availability↑

CloneID	OHu31530
Clone ID Related Accession (Same CDS sequence)	NM_031485.3 , NM_031485.4
Accession Version	NM_031485.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	glutamate-rich WD repeat-containing protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA200417.1, DA714522.1 and BC002440.2. This sequence is a reference standard in the RefSeqGene project. On May 20, 2009 this sequence version replaced NM_031485.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002440.2, SRR1803611.84474.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC GCAAGGGTCG GCGGCGCACG TGTGAAACCG GGGAACCCAT GGAAGCCGAG 
TCCGGCGACA CAAGTTCCGA GGGCCCGGCC CAGGTCTACC TGCCCGGCCG GGGGCCGCCG 
CTACGCGAAG GGGAGGAGCT GGTCATGGAC GAGGAGGCCT ATGTGCTCTA CCACCGAGCG 
CAGACTGGCG CCCCCTGTCT CAGCTTTGAC ATAGTCCGGG ATCACCTGGG AGACAACCGG 
ACAGAGCTTC CTCTTACACT TTACTTGTGT GCTGGGACCC AGGCTGAGAG CGCCCAGAGC 
AACAGACTGA TGATGCTTCG GATGCACAAT CTGCATGGGA CAAAGCCCCC ACCCTCAGAG 
GGCAGTGATG AAGAAGAAGA GGAGGAAGAT GAAGAGGATG AAGAAGAGCG GAAACCTCAG 
CTGGAGCTGG CCATGGTGCC CCACTATGGT GGCATCAACC GAGTTCGGGT GTCATGGCTG 
GGTGAAGAGC CTGTGGCTGG GGTGTGGTCA GAGAAGGGCC AGGTGGAGGT GTTTGCGCTG 
CGGCGGCTTC TGCAGGTGGT GGAGGAGCCC CAGGCCCTGG CAGCCTTCCT CCGGGATGAG 
CAGGCCCAAA TGAAGCCCAT CTTCTCCTTC GCTGGACACA TGGGCGAGGG CTTTGCCCTT 
GACTGGTCCC CCCGGGTGAC CGGTCGCCTG CTGACCGGTG ACTGTCAAAA GAACATCCAC 
CTCTGGACAC CTACGGACGG CGGCTCCTGG CACGTGGACC AGCGGCCATT CGTGGGCCAC 
ACACGCTCTG TGGAGGACCT GCAGTGGTCA CCGACTGAGA ACACGGTGTT TGCCTCCTGC 
TCAGCTGACG CCTCCATCCG CATCTGGGAC ATCCGGGCAG CCCCCAGCAA GGCCTGCATG 
CTCACCACAG CCACCGCCCA TGATGGGGAC GTCAATGTCA TCAGCTGGAG CCGCCGGGAG 
CCCTTCCTGC TCAGTGGCGG GGATGATGGG GCCCTCAAGA TCTGGGACCT TCGGCAGTTC 
AAGTCTGGTT CCCCAGTGGC CACCTTCAAG CAGCACGTGG CCCCCGTGAC CTCCGTCGAG 
TGGCACCCCC AGGACAGCGG GGTCTTTGCA GCCTCGGGTG CAGACCACCA GATCACACAG 
TGGGACCTGG CAGTGGAGCG GGACCCTGAG GCGGGCGACG TGGAGGCCGA CCCCGGACTG 
GCCGACCTCC CGCAGCAGCT GCTGTTCGTG CACCAGGGCG AGACCGAGCT GAAGGAGCTG 
CACTGGCACC CGCAGTGCCC AGGGCTCCTG GTCAGCACGG CGCTGTCAGG CTTCACCATC 
TTCCGCACCA TCAGCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113673.3
CDS	234..1574
Translation	MAARKGRRRTCETGEPMEAESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRAQTGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKPPPSEGSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFALRRLLQVVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTGDCQKNIHLWTPTDGGSWHVDQRPFVGHTRSVEDLQWSPTENTVFASCSADASIRIWDIRAAPSKACMLTTATAHDGDVNVISWSRREPFLLSGGDDGALKIWDLRQFKSGSPVATFKQHVAPVTSVEWHPQDSGVFAASGADHQITQWDLAVERDPEAGDVEADPGLADLPQQLLFVHQGETELKELHWHPQCPGLLVSTALSGFTIFRTISV

Target ORF information:

RefSeq Version	NM_031485.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate rich WD repeat containing 1 (GRWD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031485.3

ORF Insert Sequence:

ATGGCGGCGC GCAAGGGTCG GCGGCGCACG TGTGAAACCG GGGAACCCAT GGAAGCCGAG 
TCCGGCGACA CAAGTTCCGA GGGCCCGGCC CAGGTCTACC TGCCCGGCCG GGGGCCGCCG 
CTACGCGAAG GGGAGGAGCT GGTCATGGAC GAGGAGGCCT ATGTGCTCTA CCACCGAGCG 
CAGACTGGCG CCCCCTGTCT CAGCTTTGAC ATAGTCCGGG ATCACCTGGG AGACAACCGG 
ACAGAGCTTC CTCTTACACT TTACTTGTGT GCTGGGACCC AGGCTGAGAG CGCCCAGAGC 
AACAGACTGA TGATGCTTCG GATGCACAAT CTGCATGGGA CAAAGCCCCC ACCCTCAGAG 
GGCAGTGATG AAGAAGAAGA GGAGGAAGAT GAAGAGGATG AAGAAGAGCG GAAACCTCAG 
CTGGAGCTGG CCATGGTGCC CCACTATGGT GGCATCAACC GAGTTCGGGT GTCATGGCTG 
GGTGAAGAGC CTGTGGCTGG GGTGTGGTCA GAGAAGGGCC AGGTGGAGGT GTTTGCGCTG 
CGGCGGCTTC TGCAGGTGGT GGAGGAGCCC CAGGCCCTGG CAGCCTTCCT CCGGGATGAG 
CAGGCCCAAA TGAAGCCCAT CTTCTCCTTC GCTGGACACA TGGGCGAGGG CTTTGCCCTT 
GACTGGTCCC CCCGGGTGAC CGGTCGCCTG CTGACCGGTG ACTGTCAAAA GAACATCCAC 
CTCTGGACAC CTACGGACGG CGGCTCCTGG CACGTGGACC AGCGGCCATT CGTGGGCCAC 
ACACGCTCTG TGGAGGACCT GCAGTGGTCA CCGACTGAGA ACACGGTGTT TGCCTCCTGC 
TCAGCTGACG CCTCCATCCG CATCTGGGAC ATCCGGGCAG CCCCCAGCAA GGCCTGCATG 
CTCACCACAG CCACCGCCCA TGATGGGGAC GTCAATGTCA TCAGCTGGAG CCGCCGGGAG 
CCCTTCCTGC TCAGTGGCGG GGATGATGGG GCCCTCAAGA TCTGGGACCT TCGGCAGTTC 
AAGTCTGGTT CCCCAGTGGC CACCTTCAAG CAGCACGTGG CCCCCGTGAC CTCCGTCGAG 
TGGCACCCCC AGGACAGCGG GGTCTTTGCA GCCTCGGGTG CAGACCACCA GATCACACAG 
TGGGACCTGG CAGTGGAGCG GGACCCTGAG GCGGGCGACG TGGAGGCCGA CCCCGGACTG 
GCCGACCTCC CGCAGCAGCT GCTGTTCGTG CACCAGGGCG AGACCGAGCT GAAGGAGCTG 
CACTGGCACC CGCAGTGCCC AGGGCTCCTG GTCAGCACGG CGCTGTCAGG CTTCACCATC 
TTCCGCACCA TCAGCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRWD1 ( NM_031485.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_113673.3 Homo sapiens glutamate rich WD repeat containing 1 (GRWD1), mRNA.

Price & Availability↑

CloneID	OHu31530
Clone ID Related Accession (Same CDS sequence)	NM_031485.3 , NM_031485.4
Accession Version	NM_031485.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	glutamate-rich WD repeat-containing protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA714522.1, BC002440.2 and AC008403.8. On May 17, 2019 this sequence version replaced NM_031485.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.202012.1, SRR1803617.274724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000253237.10/ ENSP00000253237.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC GCAAGGGTCG GCGGCGCACG TGTGAAACCG GGGAACCCAT GGAAGCCGAG 
TCCGGCGACA CAAGTTCCGA GGGCCCGGCC CAGGTCTACC TGCCCGGCCG GGGGCCGCCG 
CTACGCGAAG GGGAGGAGCT GGTCATGGAC GAGGAGGCCT ATGTGCTCTA CCACCGAGCG 
CAGACTGGCG CCCCCTGTCT CAGCTTTGAC ATAGTCCGGG ATCACCTGGG AGACAACCGG 
ACAGAGCTTC CTCTTACACT TTACTTGTGT GCTGGGACCC AGGCTGAGAG CGCCCAGAGC 
AACAGACTGA TGATGCTTCG GATGCACAAT CTGCATGGGA CAAAGCCCCC ACCCTCAGAG 
GGCAGTGATG AAGAAGAAGA GGAGGAAGAT GAAGAGGATG AAGAAGAGCG GAAACCTCAG 
CTGGAGCTGG CCATGGTGCC CCACTATGGT GGCATCAACC GAGTTCGGGT GTCATGGCTG 
GGTGAAGAGC CTGTGGCTGG GGTGTGGTCA GAGAAGGGCC AGGTGGAGGT GTTTGCGCTG 
CGGCGGCTTC TGCAGGTGGT GGAGGAGCCC CAGGCCCTGG CAGCCTTCCT CCGGGATGAG 
CAGGCCCAAA TGAAGCCCAT CTTCTCCTTC GCTGGACACA TGGGCGAGGG CTTTGCCCTT 
GACTGGTCCC CCCGGGTGAC CGGTCGCCTG CTGACCGGTG ACTGTCAAAA GAACATCCAC 
CTCTGGACAC CTACGGACGG CGGCTCCTGG CACGTGGACC AGCGGCCATT CGTGGGCCAC 
ACACGCTCTG TGGAGGACCT GCAGTGGTCA CCGACTGAGA ACACGGTGTT TGCCTCCTGC 
TCAGCTGACG CCTCCATCCG CATCTGGGAC ATCCGGGCAG CCCCCAGCAA GGCCTGCATG 
CTCACCACAG CCACCGCCCA TGATGGGGAC GTCAATGTCA TCAGCTGGAG CCGCCGGGAG 
CCCTTCCTGC TCAGTGGCGG GGATGATGGG GCCCTCAAGA TCTGGGACCT TCGGCAGTTC 
AAGTCTGGTT CCCCAGTGGC CACCTTCAAG CAGCACGTGG CCCCCGTGAC CTCCGTCGAG 
TGGCACCCCC AGGACAGCGG GGTCTTTGCA GCCTCGGGTG CAGACCACCA GATCACACAG 
TGGGACCTGG CAGTGGAGCG GGACCCTGAG GCGGGCGACG TGGAGGCCGA CCCCGGACTG 
GCCGACCTCC CGCAGCAGCT GCTGTTCGTG CACCAGGGCG AGACCGAGCT GAAGGAGCTG 
CACTGGCACC CGCAGTGCCC AGGGCTCCTG GTCAGCACGG CGCTGTCAGG CTTCACCATC 
TTCCGCACCA TCAGCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113673.3
CDS	24..1364
Translation	MAARKGRRRTCETGEPMEAESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRAQTGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKPPPSEGSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFALRRLLQVVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTGDCQKNIHLWTPTDGGSWHVDQRPFVGHTRSVEDLQWSPTENTVFASCSADASIRIWDIRAAPSKACMLTTATAHDGDVNVISWSRREPFLLSGGDDGALKIWDLRQFKSGSPVATFKQHVAPVTSVEWHPQDSGVFAASGADHQITQWDLAVERDPEAGDVEADPGLADLPQQLLFVHQGETELKELHWHPQCPGLLVSTALSGFTIFRTISV

Target ORF information:

RefSeq Version	NM_031485.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate rich WD repeat containing 1 (GRWD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031485.4

ORF Insert Sequence:

ATGGCGGCGC GCAAGGGTCG GCGGCGCACG TGTGAAACCG GGGAACCCAT GGAAGCCGAG 
TCCGGCGACA CAAGTTCCGA GGGCCCGGCC CAGGTCTACC TGCCCGGCCG GGGGCCGCCG 
CTACGCGAAG GGGAGGAGCT GGTCATGGAC GAGGAGGCCT ATGTGCTCTA CCACCGAGCG 
CAGACTGGCG CCCCCTGTCT CAGCTTTGAC ATAGTCCGGG ATCACCTGGG AGACAACCGG 
ACAGAGCTTC CTCTTACACT TTACTTGTGT GCTGGGACCC AGGCTGAGAG CGCCCAGAGC 
AACAGACTGA TGATGCTTCG GATGCACAAT CTGCATGGGA CAAAGCCCCC ACCCTCAGAG 
GGCAGTGATG AAGAAGAAGA GGAGGAAGAT GAAGAGGATG AAGAAGAGCG GAAACCTCAG 
CTGGAGCTGG CCATGGTGCC CCACTATGGT GGCATCAACC GAGTTCGGGT GTCATGGCTG 
GGTGAAGAGC CTGTGGCTGG GGTGTGGTCA GAGAAGGGCC AGGTGGAGGT GTTTGCGCTG 
CGGCGGCTTC TGCAGGTGGT GGAGGAGCCC CAGGCCCTGG CAGCCTTCCT CCGGGATGAG 
CAGGCCCAAA TGAAGCCCAT CTTCTCCTTC GCTGGACACA TGGGCGAGGG CTTTGCCCTT 
GACTGGTCCC CCCGGGTGAC CGGTCGCCTG CTGACCGGTG ACTGTCAAAA GAACATCCAC 
CTCTGGACAC CTACGGACGG CGGCTCCTGG CACGTGGACC AGCGGCCATT CGTGGGCCAC 
ACACGCTCTG TGGAGGACCT GCAGTGGTCA CCGACTGAGA ACACGGTGTT TGCCTCCTGC 
TCAGCTGACG CCTCCATCCG CATCTGGGAC ATCCGGGCAG CCCCCAGCAA GGCCTGCATG 
CTCACCACAG CCACCGCCCA TGATGGGGAC GTCAATGTCA TCAGCTGGAG CCGCCGGGAG 
CCCTTCCTGC TCAGTGGCGG GGATGATGGG GCCCTCAAGA TCTGGGACCT TCGGCAGTTC 
AAGTCTGGTT CCCCAGTGGC CACCTTCAAG CAGCACGTGG CCCCCGTGAC CTCCGTCGAG 
TGGCACCCCC AGGACAGCGG GGTCTTTGCA GCCTCGGGTG CAGACCACCA GATCACACAG 
TGGGACCTGG CAGTGGAGCG GGACCCTGAG GCGGGCGACG TGGAGGCCGA CCCCGGACTG 
GCCGACCTCC CGCAGCAGCT GCTGTTCGTG CACCAGGGCG AGACCGAGCT GAAGGAGCTG 
CACTGGCACC CGCAGTGCCC AGGGCTCCTG GTCAGCACGG CGCTGTCAGG CTTCACCATC 
TTCCGCACCA TCAGCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis.
EMBO reports18(1)123-137(2017 01)
Kayama K,Watanabe S,Takafuji T,Tsuji T,Hironaka K,Matsumoto M,Nakayama KI,Enari M,Kohno T,Shiraishi K,Kiyono T,Yoshida K,Sugimoto N,Fujita M

Nucleosome assembly and disassembly activity of GRWD1, a novel Cdt1-binding protein that promotes pre-replication complex formation.
Biochimica et biophysica acta1863(11)2739-2748(2016 11)
Aizawa M,Sugimoto N,Watanabe S,Yoshida K,Fujita M

Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture.
Nucleic acids research43(12)5898-911(2015 Jul)
Sugimoto N,Maehara K,Yoshida K,Yasukouchi S,Osano S,Watanabe S,Aizawa M,Yugawa T,Kiyono T,Kurumizaka H,Ohkawa Y,Fujita M

A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
PLoS genetics9(1)e1003210(2013)
Cloutier P,Lavall?e-Adam M,Faubert D,Blanchette M,Coulombe B

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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GRWD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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