H2AFB3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	H2AFB3
Entrez Gene ID	83740
Full Name	H2A histone family member B3
Synonyms	H2ABBD,H2AFB
General protein information	Preferred Names H2A histone family member B3 Names histone H2A-Bbd type 2/3 H2A Barr body-deficient H2A.Bbd histone variant H2A, Barr-body deficient
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq28
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the most telomeric copy. [provided by RefSeq, Oct 2015].
Disorder MIM:	300445

mRNA and Protein(s)

mRNA	Protein	Name
NM_080720.2	NP_542451.1	histone H2A-Bbd type 2/3
NM_080720.1	NP_542451.1	histone H2A-Bbd type 2/3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC612656	XP_855480.2
Bos taurus (cattle)	LOC528006	NP_001069373.1
Mus musculus (house mouse)	H2afb2-ps	NP_001268459.1
Mus musculus (house mouse)	H2afb3-ps	NP_001268460.1
Rattus norvegicus (Norway rat)	H2afb3	NP_001100439.1
	H2AFB3	XP_001091351.1
Canis lupus familiaris (dog)	LOC102152481	XP_005642100.1
Homo sapiens (human)	H2AFB3	NP_542451.1
	LOC722826	XP_001118919.1
Mus musculus (house mouse)	Gm14920	NP_001096135.1
Homo sapiens (human)	H2AFB1	NP_001017990.1
Homo sapiens (human)	H2AFB2	NP_001017991.1

Gene Ontology
Bibliography

Related articles in PubMed

Nucleosome adaptability conferred by sequence and structural variations in histone H2A-H2B dimers.
Shaytan AK, Landsman D, Panchenko AR
Current opinion in structural biology3248-57(2015 Jun)

Mammal-specific H2A variant, H2ABbd, is involved in apoptotic induction via activation of NF-?B signaling pathway.
Goshima T, Shimada M, Sharif J, Matsuo H, Misaki T, Johmura Y, Murata K, Koseki H, Nakanishi M
The Journal of biological chemistry289(17)11656-66(2014 Apr)

Histone variant H2A.Bbd is associated with active transcription and mRNA processing in human cells.
Tolstorukov MY, Goldman JA, Gilbert C, Ogryzko V, Kingston RE, Park PJ
Molecular cell47(4)596-607(2012 Aug)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Assembly and disassembly of nucleosome core particles containing histone variants by human nucleosome assembly protein I.
Okuwaki M, Kato K, Shimahara H, Tate S, Nagata K
Molecular and cellular biology25(23)10639-51(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data suggest that H2ABbd may contribute to specific chromatin structures and promote NF-kappaB activation, which could in turn induce apoptosis in mammalian cells
Title: Mammal-specific H2A variant, H2ABbd, is involved in apoptotic induction via activation of NF-?B signaling pathway.

The following H2AFB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H2AFB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10432	NM_080720.2 Latest version!	Homo sapiens H2A.B variant histone 3 (H2AB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu10432	NM_080720.1	Homo sapiens H2A histone family member B3 (H2AFB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H2AFB3 ( NM_080720.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_542451.1 Homo sapiens H2A.B variant histone 3 (H2AB3), mRNA.

Price & Availability↑

CloneID	OHu10432
Clone ID Related Accession (Same CDS sequence)	NM_080720.2 , NM_080720.1
Accession Version	NM_080720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	histone H2A-Bbd type 2/3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX571846.3. On Dec 3, 2019 this sequence version replaced NM_080720.1. ##Evidence-Data-START## Transcript is intronless :: AL602062.1, BU740141.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-independent histone :: PMID: 25731851 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGAGGA GGAGGAGACG CCGAGGGTCC TCCGGTGCTG GCGGCCGGGG GCGGACCTGC 
TCTCGCACCG TCCGAGCGGA GCTTTCGTTT TCAGTGAGCC AGGTGGAGCG CAGTCTACGG 
GAGGGCCACT ACGCTCAGCG CCTGAGTCGC ACGGCGCCGG TCTACCTCGC TGCGGTTATT 
GAGTACCTGA CGGCCAAGGT CCTGGAGCTG GCGGGCAACG AGGCCCAGAA CAGCGGAGAG 
CGGAACATCA CTCCCCTGCT GCTGGACATG GTGGTTCACA ACGACAGGCT ACTGAGCACC 
CTTTTCAACA CGACCACCAT CTCTCAAGTG GCCCCTGGCG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542451.1
CDS	79..426
Translation	MPRRRRRRGSSGAGGRGRTCSRTVRAELSFSVSQVERSLREGHYAQRLSRTAPVYLAAVIEYLTAKVLELAGNEAQNSGERNITPLLLDMVVHNDRLLSTLFNTTTISQVAPGED

Target ORF information:

RefSeq Version	NM_080720.2
Organism	Homo sapiens(human)
Definition	Homo sapiens H2A.B variant histone 3 (H2AB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080720.2

ORF Insert Sequence:

ATGCCGAGGA GGAGGAGACG CCGAGGGTCC TCCGGTGCTG GCGGCCGGGG GCGGACCTGC 
TCTCGCACCG TCCGAGCGGA GCTTTCGTTT TCAGTGAGCC AGGTGGAGCG CAGTCTACGG 
GAGGGCCACT ACGCTCAGCG CCTGAGTCGC ACGGCGCCGG TCTACCTCGC TGCGGTTATT 
GAGTACCTGA CGGCCAAGGT CCTGGAGCTG GCGGGCAACG AGGCCCAGAA CAGCGGAGAG 
CGGAACATCA CTCCCCTGCT GCTGGACATG GTGGTTCACA ACGACAGGCT ACTGAGCACC 
CTTTTCAACA CGACCACCAT CTCTCAAGTG GCCCCTGGCG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H2AFB3 ( NM_080720.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_542451.1 Homo sapiens H2A histone family member B3 (H2AFB3), mRNA.

Price & Availability↑

CloneID	OHu10432
Clone ID Related Accession (Same CDS sequence)	NM_080720.2 , NM_080720.1
Accession Version	NM_080720.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-06
Organism	Homo sapiens(human)
Product	histone H2A-Bbd type 2/3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF254576.1. On May 6, 2005 this sequence version replaced XM_373381.1. ##Evidence-Data-START## Transcript is intronless :: AF254576.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-independent histone :: PMID: 25731851 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGAGGA GGAGGAGACG CCGAGGGTCC TCCGGTGCTG GCGGCCGGGG GCGGACCTGC 
TCTCGCACCG TCCGAGCGGA GCTTTCGTTT TCAGTGAGCC AGGTGGAGCG CAGTCTACGG 
GAGGGCCACT ACGCTCAGCG CCTGAGTCGC ACGGCGCCGG TCTACCTCGC TGCGGTTATT 
GAGTACCTGA CGGCCAAGGT CCTGGAGCTG GCGGGCAACG AGGCCCAGAA CAGCGGAGAG 
CGGAACATCA CTCCCCTGCT GCTGGACATG GTGGTTCACA ACGACAGGCT ACTGAGCACC 
CTTTTCAACA CGACCACCAT CTCTCAAGTG GCCCCTGGCG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542451.1
CDS	9..356
Translation	MPRRRRRRGSSGAGGRGRTCSRTVRAELSFSVSQVERSLREGHYAQRLSRTAPVYLAAVIEYLTAKVLELAGNEAQNSGERNITPLLLDMVVHNDRLLSTLFNTTTISQVAPGED

Target ORF information:

RefSeq Version	NM_080720.1
Organism	Homo sapiens(human)
Definition	Homo sapiens H2A histone family member B3 (H2AFB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080720.1

ORF Insert Sequence:

ATGCCGAGGA GGAGGAGACG CCGAGGGTCC TCCGGTGCTG GCGGCCGGGG GCGGACCTGC 
TCTCGCACCG TCCGAGCGGA GCTTTCGTTT TCAGTGAGCC AGGTGGAGCG CAGTCTACGG 
GAGGGCCACT ACGCTCAGCG CCTGAGTCGC ACGGCGCCGG TCTACCTCGC TGCGGTTATT 
GAGTACCTGA CGGCCAAGGT CCTGGAGCTG GCGGGCAACG AGGCCCAGAA CAGCGGAGAG 
CGGAACATCA CTCCCCTGCT GCTGGACATG GTGGTTCACA ACGACAGGCT ACTGAGCACC 
CTTTTCAACA CGACCACCAT CTCTCAAGTG GCCCCTGGCG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nucleosome adaptability conferred by sequence and structural variations in histone H2A-H2B dimers.
Current opinion in structural biology3248-57(2015 Jun)
Shaytan AK,Landsman D,Panchenko AR

Mammal-specific H2A variant, H2ABbd, is involved in apoptotic induction via activation of NF-?B signaling pathway.
The Journal of biological chemistry289(17)11656-66(2014 Apr)
Goshima T,Shimada M,Sharif J,Matsuo H,Misaki T,Johmura Y,Murata K,Koseki H,Nakanishi M

Histone variant H2A.Bbd is associated with active transcription and mRNA processing in human cells.
Molecular cell47(4)596-607(2012 Aug)
Tolstorukov MY,Goldman JA,Gilbert C,Ogryzko V,Kingston RE,Park PJ

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Assembly and disassembly of nucleosome core particles containing histone variants by human nucleosome assembly protein I.
Molecular and cellular biology25(23)10639-51(2005 Dec)
Okuwaki M,Kato K,Shimahara H,Tate S,Nagata K

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H2AFB3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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