H4C14 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	H4C14
Entrez Gene ID	8370
Full Name	histone cluster 2 H4 family member a
Synonyms	FO108,H4,H4/n,H4F2,H4FN,HIST2H4
General protein information	Preferred Names histone cluster 2 H4 family member a Names histone H4 H4 histone family, member N H4 histone, family 2 histone 2, H4a histone IV, family 2 histone cluster 2, H4a
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.2
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Aug 2015].
Disorder MIM:	142750

mRNA and Protein(s)

mRNA	Protein	Name
NM_003548.2	NP_003539.1	histone H4

Pathways from BioSystems

Homologies

Homo sapiens (human)	HIST2H4A	NP_003539.1
Oryza sativa (rice)	Os04g0583600	NP_001053664.1
Xenopus tropicalis (tropical clawed frog)	LOC101734147	XP_004917053.1
Pan troglodytes (chimpanzee)	LOC736707	XP_001138202.1
Bos taurus (cattle)	H4	NP_776305.1
Arabidopsis thaliana (thale cress)	AT1G07820	NP_563797.1
Mus musculus (house mouse)	Hist1h4b	NP_835500.1
Rattus norvegicus (Norway rat)	LOC100912564	XP_003749904.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005026	XP_307600.4
Caenorhabditis elegans (roundworm)	his-64	NP_502154.1
	LOC699317	XP_001089711.1
Drosophila melanogaster (fruit fly)	His4:CG33905	NP_001027293.1
Canis lupus familiaris (dog)	LOC486670	XP_543797.2
Danio rerio (zebrafish)	LOC101884830	XP_005168859.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The TIP60 Complex Regulates Bivalent Chromatin Recognition by 53BP1 through Direct H4K20me Binding and H2AK15 Acetylation.
Jacquet K, Fradet-Turcotte A, Avvakumov N, Lambert JP, Roques C, Pandita RK, Paquet E, Herst P, Gingras AC, Pandita TK, Legube G, Doyon Y, Durocher D, C?t? J
Molecular cell62(3)409-421(2016 05)

Functional characterization of a human histone gene cluster duplication.
Braastad CD, Hovhannisyan H, van Wijnen AJ, Stein JL, Stein GS
Gene342(1)35-40(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NASP balances the activity of the heat shock proteins Hsc70 and Hsp90 to direct H3-H4 for degradation by chaperone-mediated autophagy
Title: A specific function for the histone chaperone NASP to fine-tune a reservoir of soluble H3-H4 in the histone supply chain.

H2B and H4 histones were mobilized during herpes simplex virus 1 infection and became available to bind to viral genomes.
Title: Core histones H2B and H4 are mobilized during infection with herpes simplex virus 1.

Loss of the H4 arginine 3 methylation mark through short hairpin RNA-mediated knockdown of PRMT5 leads to reduced DNMT3A binding, loss of DNA methylation and gene activation.
Title: PRMT5-mediated methylation of histone H4R3 recruits DNMT3A, coupling histone and DNA methylation in gene silencing.

deiminated residues were present in H2A (1-56) and H4 (1-52)
Title: Deimination of histone H2A and H4 at arginine 3 in HL-60 granulocytes.

assessed the functional coupling between chromatin organization and regulation of histone H4/n gene expression during HL-60 differentiation into the monocyte/macrophage lineage
Title: Maintenance of open chromatin and selective genomic occupancy at the cell cycle-regulated histone H4 promoter during differentiation of HL-60 promyelocytic leukemia cells.

The following H4C14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H4C14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu02087	NM_003548.2 Latest version!	Homo sapiens H4 clustered histone 14 (H4C14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H4C14 ( NM_003548.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003539.1 Homo sapiens H4 clustered histone 14 (H4C14), mRNA.

Price & Availability↑

CloneID	OHu02087
Clone ID Related Accession (Same CDS sequence)	NM_003548.2
Accession Version	NM_003548.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-02
Organism	Homo sapiens(human)
Product	histone H4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC239868.2. On or before Sep 20, 2006 this sequence version replaced XM_001124639.1, NM_003548.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: DB014249.1, DA456913.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGCA GAGGAAAGGG CGGAAAAGGC TTAGGCAAAG GGGGCGCTAA GCGCCACCGC 
AAGGTCTTGA GAGACAACAT TCAGGGCATC ACCAAGCCTG CCATTCGGCG TCTAGCTCGG 
CGTGGCGGCG TTAAGCGGAT CTCTGGCCTC ATTTACGAGG AGACCCGCGG TGTGCTGAAG 
GTGTTCCTGG AGAATGTGAT TCGGGACGCA GTCACCTACA CCGAGCACGC CAAGCGCAAG 
ACCGTCACAG CCATGGATGT GGTGTACGCG CTCAAGCGCC AGGGGCGCAC CCTGTACGGC 
TTCGGAGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003539.1
CDS	29..340
Translation	MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

Target ORF information:

RefSeq Version	NM_003548.2
Organism	Homo sapiens(human)
Definition	Homo sapiens H4 clustered histone 14 (H4C14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003548.2

ORF Insert Sequence:

ATGTCCGGCA GAGGAAAGGG CGGAAAAGGC TTAGGCAAAG GGGGCGCTAA GCGCCACCGC 
AAGGTCTTGA GAGACAACAT TCAGGGCATC ACCAAGCCTG CCATTCGGCG TCTAGCTCGG 
CGTGGCGGCG TTAAGCGGAT CTCTGGCCTC ATTTACGAGG AGACCCGCGG TGTGCTGAAG 
GTGTTCCTGG AGAATGTGAT TCGGGACGCA GTCACCTACA CCGAGCACGC CAAGCGCAAG 
ACCGTCACAG CCATGGATGT GGTGTACGCG CTCAAGCGCC AGGGGCGCAC CCTGTACGGC 
TTCGGAGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The TIP60 Complex Regulates Bivalent Chromatin Recognition by 53BP1 through Direct H4K20me Binding and H2AK15 Acetylation.
Molecular cell62(3)409-421(2016 05)
Jacquet K,Fradet-Turcotte A,Avvakumov N,Lambert JP,Roques C,Pandita RK,Paquet E,Herst P,Gingras AC,Pandita TK,Legube G,Doyon Y,Durocher D,C?t? J

Functional characterization of a human histone gene cluster duplication.
Gene342(1)35-40(2004 Nov)
Braastad CD,Hovhannisyan H,van Wijnen AJ,Stein JL,Stein GS

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H4C14 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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