SELENOO cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SELENOO
Entrez Gene ID	83642
Full Name	selenoprotein O
Synonyms	SELO
General protein information	Preferred Names selenoprotein O Names selenoprotein O
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.33
Summary	This gene encodes a selenoprotein that is localized to the mitochondria. It is the largest mammalian selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The exact function of this selenoprotein is not known, but it is thought to have redox activity. [provided by RefSeq, Dec 2016].
Disorder MIM:	607917

mRNA and Protein(s)

mRNA	Protein	Name
NM_031454.1	NP_113642.1	selenoprotein O
NM_031454.2	NP_113642.1	protein adenylyltransferase SelO, mitochondrial

Pathways from BioSystems

Homologies

	SELO	NP_001152883.1
Oryza sativa (rice)	Os06g0320700	NP_001057514.1
Bos taurus (cattle)	SELO	NP_001156665.1
Mus musculus (house mouse)	Selo	NP_082181.2
Gallus gallus (chicken)	SELO	NP_001108489.1
Arabidopsis thaliana (thale cress)	AT5G13030	NP_196807.2
Xenopus tropicalis (tropical clawed frog)	selo	NP_001135537.2
Rattus norvegicus (Norway rat)	Selo	NP_001078954.1
Pan troglodytes (chimpanzee)	SELO	XP_003317367.2
Homo sapiens (human)	SELO	NP_113642.1
Canis lupus familiaris (dog)	SELO	NP_001131075.1
Danio rerio (zebrafish)	selo	NP_001038336.2

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Characterization of mammalian selenoprotein o: a redox-active mitochondrial protein.
Han SJ, Lee BC, Yim SH, Gladyshev VN, Lee SR
PloS one9(4)e95518(2014)

A novel protein kinase-like domain in a selenoprotein, widespread in the tree of life.
Dudkiewicz M, Szczepi?ska T, Grynberg M, Paw?owski K
PloS one7(2)e32138(2012)

A genome annotation-driven approach to cloning the human ORFeome.
Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I
Genome biology5(10)R84(2004)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SelO is a redox-active mitochondrial selenoprotein.
Title: Characterization of mammalian selenoprotein o: a redox-active mitochondrial protein.

The SELO protein has a protein domain with significant albeit remote sequence similarity to protein kinase-like proteins.
Title: Serum lipid and lipoprotein levels in Japanese patients with familial hypercholesterolemia.

The following SELENOO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SELENOO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29788	NM_031454.2 Latest version!	Homo sapiens selenoprotein O (SELENOO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu29788	NM_031454.1	Homo sapiens selenoprotein O (SELENOO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SELENOO ( NM_031454.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_113642.1 Homo sapiens selenoprotein O (SELENOO), mRNA.

Price & Availability↑

CloneID	OHu29788
Clone ID Related Accession (Same CDS sequence)	NM_031454.1 , NM_031454.2
Accession Version	NM_031454.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Homo sapiens(human)
Product	selenoprotein O
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY324823.1. ##Evidence-Data-START## Transcript exon combination :: AY324823.1, SRR1163657.420242.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 24751718 protein contains selenocysteine :: PMID: 12775843 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGTAT ACAGGGCAGC GCTCGGGGCT TCGCTCGCGG CTGCCCGACT CTTGCCCCTC 
GGTCGCTGTT CCCCGTCGCC GGCGCCCCGC TCTACGTTGT CGGGCGCCGC CATGGAGCCC 
GCGCCTCGCT GGCTGGCGGG GCTGCGCTTC GACAACCGCG CCCTGCGCGC CCTGCCCGTG 
GAGGCGCCGC CGCCCGGTCC CGAGGGCGCC CCGTCCGCGC CGCGGCCCGT GCCCGGGGCC 
TGCTTCACCC GCGTGCAGCC CACCCCGCTG CGGCAGCCGC GCCTCGTGGC GCTGTCAGAG 
CCCGCGCTGG CGTTGCTGGG CCTGGGCGCG CCGCCCGCGC GCGAGGCCGA GGCCGAGGCC 
GCGCTGTTCT TCAGCGGCAA CGCGCTCCTG CCGGGCGCCG AGCCCGCCGC GCACTGCTAC 
TGCGGCCACC AATTCGGCCA GTTCGCCGGG CAGCTGGGCG ACGGCGCCGC CATGTACCTG 
GGCGAGGTGT GCACGGCGAC CGGCGAGCGC TGGGAGCTGC AGCTCAAGGG CGCCGGGCCC 
ACGCCCTTCT CCAGACAGGC CGACGGTCGC AAGGTCCTAC GGTCAAGCAT CCGGGAGTTT 
CTATGCAGCG AAGCCATGTT CCACCTGGGA GTCCCCACCA CACGGGCCGG CGCCTGCGTC 
ACGTCCGAGT CCACGGTGGT GCGCGACGTG TTCTATGATG GTAATCCCAA ATATGAACAA 
TGCACGGTTG TGTTGCGTGT AGCTTCCACT TTCATAAGGT TTGGATCCTT TGAGATTTTT 
AAGTCTGCAG ATGAGCACAC AGGGCGTGCA GGCCCCAGCG TGGGGAGGAA CGACATTCGA 
GTGCAGCTGC TCGACTATGT CATCAGCTCC TTTTACCCCG AGATCCAGGC TGCTCATGCC 
AGCGACAGCG TGCAGAGAAA TGCTGCCTTC TTCCGGGAGG TGACGCGGCG CACGGCGCGG 
ATGGTGGCCG AGTGGCAGTG TGTGGGCTTC TGCCACGGCG TGCTCAACAC CGACAACATG 
AGCATCCTGG GGCTCACCAT CGACTACGGG CCCTTTGGCT TCCTGGACAG GTACGACCCC 
GACCACGTGT GCAATGCCTC CGACAACACC GGCCGCTACG CGTACAGCAA GCAGCCCGAG 
GTGTGCAGGT GGAACCTGCG GAAGCTGGCC GAGGCCCTGC AGCCGGAACT GCCCCTGGAG 
CTGGGGGAGG CCATCCTGGC CGAGGAGTTT GACGCCGAGT TCCAAAGGCA CTACCTGCAG 
AAGATGCGCA GGAAGCTGGG CCTCGTGCAG GTGGAGCTGG AGGAAGACGG GGCGCTGGTG 
TCCAAGCTCC TGGAGACCAT GCATCTGACC GGTGCCGACT TCACAAACAC CTTCTACTTG 
CTGAGCTCCT TCCCAGTGGA GCTAGAGTCG CCAGGCCTGG CGGAATTCCT GGCCAGGCTG 
ATGGAGCAGT GTGCCTCCCT GGAGGAGCTG AGGCTGGCCT TCCGGCCCCA GATGGATCCC 
CGGCAGCTAT CCATGATGCT GATGCTGGCG CAGTCAAACC CGCAGCTGTT CGCGCTTATG 
GGCACCCGGG CAGGCATCGC CAGGGAGCTG GAGCGTGTGG AGCAGCAGTC TCGGCTGGAG 
CAGCTGAGTG CGGCAGAGCT GCAGAGCAGG AACCAGGGCC ACTGGGCTGA CTGGCTACAG 
GCGTACAGAG CCCGGCTGGA CAAGGACCTG GAAGGCGCTG GGGACGCTGC CGCCTGGCAG 
GCTGAGCACG TGCGCGTGAT GCACGCCAAC AACCCGAAGT ACGTGCTGAG GAACTACATC 
GCGCAGAATG CCATCGAGGC TGCCGAGCGC GGGGACTTCT CAGAGGTGCG GCGGGTGCTG 
AAACTACTGG AGACCCCTTA CCACTGCGAG GCGGGGGCCG CCACAGACGC CGAGGCCACG 
GAAGCCGACG GGGCGGACGG CAGGCAGCGC TCCTACAGCA GTAAGCCCCC GCTCTGGGCA 
GCAGAACTGT GCGTGACATG ATCTTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113642.1
CDS	59..2068
Translation	MAVYRAALGASLAAARLLPLGRCSPSPAPRSTLSGAAMEPAPRWLAGLRFDNRALRALPVEAPPPGPEGAPSAPRPVPGACFTRVQPTPLRQPRLVALSEPALALLGLGAPPAREAEAEAALFFSGNALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTATGERWELQLKGAGPTPFSRQADGRKVLRSSIREFLCSEAMFHLGVPTTRAGACVTSESTVVRDVFYDGNPKYEQCTVVLRVASTFIRFGSFEIFKSADEHTGRAGPSVGRNDIRVQLLDYVISSFYPEIQAAHASDSVQRNAAFFREVTRRTARMVAEWQCVGFCHGVLNTDNMSILGLTIDYGPFGFLDRYDPDHVCNASDNTGRYAYSKQPEVCRWNLRKLAEALQPELPLELGEAILAEEFDAEFQRHYLQKMRRKLGLVQVELEEDGALVSKLLETMHLTGADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDPRQLSMMLMLAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQAYRARLDKDLEGAGDAAAWQAEHVRVMHANNPKYVLRNYIAQNAIEAAERGDFSEVRRVLKLLETPYHCEAGAATDAEATEADGADGRQRSYSSKPPLWAAELCVT*SS

Target ORF information:

RefSeq Version	NM_031454.1
Organism	Homo sapiens(human)
Definition	Homo sapiens selenoprotein O (SELENOO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031454.1

ORF Insert Sequence:

ATGGCCGTAT ACAGGGCAGC GCTCGGGGCT TCGCTCGCGG CTGCCCGACT CTTGCCCCTC 
GGTCGCTGTT CCCCGTCGCC GGCGCCCCGC TCTACGTTGT CGGGCGCCGC CATGGAGCCC 
GCGCCTCGCT GGCTGGCGGG GCTGCGCTTC GACAACCGCG CCCTGCGCGC CCTGCCCGTG 
GAGGCGCCGC CGCCCGGTCC CGAGGGCGCC CCGTCCGCGC CGCGGCCCGT GCCCGGGGCC 
TGCTTCACCC GCGTGCAGCC CACCCCGCTG CGGCAGCCGC GCCTCGTGGC GCTGTCAGAG 
CCCGCGCTGG CGTTGCTGGG CCTGGGCGCG CCGCCCGCGC GCGAGGCCGA GGCCGAGGCC 
GCGCTGTTCT TCAGCGGCAA CGCGCTCCTG CCGGGCGCCG AGCCCGCCGC GCACTGCTAC 
TGCGGCCACC AATTCGGCCA GTTCGCCGGG CAGCTGGGCG ACGGCGCCGC CATGTACCTG 
GGCGAGGTGT GCACGGCGAC CGGCGAGCGC TGGGAGCTGC AGCTCAAGGG CGCCGGGCCC 
ACGCCCTTCT CCAGACAGGC CGACGGTCGC AAGGTCCTAC GGTCAAGCAT CCGGGAGTTT 
CTATGCAGCG AAGCCATGTT CCACCTGGGA GTCCCCACCA CACGGGCCGG CGCCTGCGTC 
ACGTCCGAGT CCACGGTGGT GCGCGACGTG TTCTATGATG GTAATCCCAA ATATGAACAA 
TGCACGGTTG TGTTGCGTGT AGCTTCCACT TTCATAAGGT TTGGATCCTT TGAGATTTTT 
AAGTCTGCAG ATGAGCACAC AGGGCGTGCA GGCCCCAGCG TGGGGAGGAA CGACATTCGA 
GTGCAGCTGC TCGACTATGT CATCAGCTCC TTTTACCCCG AGATCCAGGC TGCTCATGCC 
AGCGACAGCG TGCAGAGAAA TGCTGCCTTC TTCCGGGAGG TGACGCGGCG CACGGCGCGG 
ATGGTGGCCG AGTGGCAGTG TGTGGGCTTC TGCCACGGCG TGCTCAACAC CGACAACATG 
AGCATCCTGG GGCTCACCAT CGACTACGGG CCCTTTGGCT TCCTGGACAG GTACGACCCC 
GACCACGTGT GCAATGCCTC CGACAACACC GGCCGCTACG CGTACAGCAA GCAGCCCGAG 
GTGTGCAGGT GGAACCTGCG GAAGCTGGCC GAGGCCCTGC AGCCGGAACT GCCCCTGGAG 
CTGGGGGAGG CCATCCTGGC CGAGGAGTTT GACGCCGAGT TCCAAAGGCA CTACCTGCAG 
AAGATGCGCA GGAAGCTGGG CCTCGTGCAG GTGGAGCTGG AGGAAGACGG GGCGCTGGTG 
TCCAAGCTCC TGGAGACCAT GCATCTGACC GGTGCCGACT TCACAAACAC CTTCTACTTG 
CTGAGCTCCT TCCCAGTGGA GCTAGAGTCG CCAGGCCTGG CGGAATTCCT GGCCAGGCTG 
ATGGAGCAGT GTGCCTCCCT GGAGGAGCTG AGGCTGGCCT TCCGGCCCCA GATGGATCCC 
CGGCAGCTAT CCATGATGCT GATGCTGGCG CAGTCAAACC CGCAGCTGTT CGCGCTTATG 
GGCACCCGGG CAGGCATCGC CAGGGAGCTG GAGCGTGTGG AGCAGCAGTC TCGGCTGGAG 
CAGCTGAGTG CGGCAGAGCT GCAGAGCAGG AACCAGGGCC ACTGGGCTGA CTGGCTACAG 
GCGTACAGAG CCCGGCTGGA CAAGGACCTG GAAGGCGCTG GGGACGCTGC CGCCTGGCAG 
GCTGAGCACG TGCGCGTGAT GCACGCCAAC AACCCGAAGT ACGTGCTGAG GAACTACATC 
GCGCAGAATG CCATCGAGGC TGCCGAGCGC GGGGACTTCT CAGAGGTGCG GCGGGTGCTG 
AAACTACTGG AGACCCCTTA CCACTGCGAG GCGGGGGCCG CCACAGACGC CGAGGCCACG 
GAAGCCGACG GGGCGGACGG CAGGCAGCGC TCCTACAGCA GTAAGCCCCC GCTCTGGGCA 
GCAGAACTGT GCGTGACATG ATCTTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SELENOO ( NM_031454.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_113642.1 Homo sapiens selenoprotein O (SELENOO), mRNA.

Price & Availability↑

CloneID	OHu29788
Clone ID Related Accession (Same CDS sequence)	NM_031454.1 , NM_031454.2
Accession Version	NM_031454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	protein adenylyltransferase SelO, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY324823.1. On Nov 22, 2018 this sequence version replaced NM_031454.1. ##Evidence-Data-START## Transcript exon combination :: BC110866.1, AY324823.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 24751718 MANE Ensembl match :: ENST00000380903.7/ ENSP00000370288.2 protein contains selenocysteine :: PMID: 12775843 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGTAT ACAGGGCAGC GCTCGGGGCT TCGCTCGCGG CTGCCCGACT CTTGCCCCTC 
GGTCGCTGTT CCCCGTCGCC GGCGCCCCGC TCTACGTTGT CGGGCGCCGC CATGGAGCCC 
GCGCCTCGCT GGCTGGCGGG GCTGCGCTTC GACAACCGCG CCCTGCGCGC CCTGCCCGTG 
GAGGCGCCGC CGCCCGGTCC CGAGGGCGCC CCGTCCGCGC CGCGGCCCGT GCCCGGGGCC 
TGCTTCACCC GCGTGCAGCC CACCCCGCTG CGGCAGCCGC GCCTCGTGGC GCTGTCAGAG 
CCCGCGCTGG CGTTGCTGGG CCTGGGCGCG CCGCCCGCGC GCGAGGCCGA GGCCGAGGCC 
GCGCTGTTCT TCAGCGGCAA CGCGCTCCTG CCGGGCGCCG AGCCCGCCGC GCACTGCTAC 
TGCGGCCACC AATTCGGCCA GTTCGCCGGG CAGCTGGGCG ACGGCGCCGC CATGTACCTG 
GGCGAGGTGT GCACGGCGAC CGGCGAGCGC TGGGAGCTGC AGCTCAAGGG CGCCGGGCCC 
ACGCCCTTCT CCAGACAGGC CGACGGTCGC AAGGTCCTAC GGTCAAGCAT CCGGGAGTTT 
CTATGCAGCG AAGCCATGTT CCACCTGGGA GTCCCCACCA CACGGGCCGG CGCCTGCGTC 
ACGTCCGAGT CCACGGTGGT GCGCGACGTG TTCTATGATG GTAATCCCAA ATATGAACAA 
TGCACGGTTG TGTTGCGTGT AGCTTCCACT TTCATAAGGT TTGGATCCTT TGAGATTTTT 
AAGTCTGCAG ATGAGCACAC AGGGCGTGCA GGCCCCAGCG TGGGGAGGAA CGACATTCGA 
GTGCAGCTGC TCGACTATGT CATCAGCTCC TTTTACCCCG AGATCCAGGC TGCTCATGCC 
AGCGACAGCG TGCAGAGAAA TGCTGCCTTC TTCCGGGAGG TGACGCGGCG CACGGCGCGG 
ATGGTGGCCG AGTGGCAGTG TGTGGGCTTC TGCCACGGCG TGCTCAACAC CGACAACATG 
AGCATCCTGG GGCTCACCAT CGACTACGGG CCCTTTGGCT TCCTGGACAG GTACGACCCC 
GACCACGTGT GCAATGCCTC CGACAACACC GGCCGCTACG CGTACAGCAA GCAGCCCGAG 
GTGTGCAGGT GGAACCTGCG GAAGCTGGCC GAGGCCCTGC AGCCGGAACT GCCCCTGGAG 
CTGGGGGAGG CCATCCTGGC CGAGGAGTTT GACGCCGAGT TCCAAAGGCA CTACCTGCAG 
AAGATGCGCA GGAAGCTGGG CCTCGTGCAG GTGGAGCTGG AGGAAGACGG GGCGCTGGTG 
TCCAAGCTCC TGGAGACCAT GCATCTGACC GGTGCCGACT TCACAAACAC CTTCTACTTG 
CTGAGCTCCT TCCCAGTGGA GCTAGAGTCG CCAGGCCTGG CGGAATTCCT GGCCAGGCTG 
ATGGAGCAGT GTGCCTCCCT GGAGGAGCTG AGGCTGGCCT TCCGGCCCCA GATGGATCCC 
CGGCAGCTAT CCATGATGCT GATGCTGGCG CAGTCAAACC CGCAGCTGTT CGCGCTTATG 
GGCACCCGGG CAGGCATCGC CAGGGAGCTG GAGCGTGTGG AGCAGCAGTC TCGGCTGGAG 
CAGCTGAGTG CGGCAGAGCT GCAGAGCAGG AACCAGGGCC ACTGGGCTGA CTGGCTACAG 
GCGTACAGAG CCCGGCTGGA CAAGGACCTG GAAGGCGCTG GGGACGCTGC CGCCTGGCAG 
GCTGAGCACG TGCGCGTGAT GCACGCCAAC AACCCGAAGT ACGTGCTGAG GAACTACATC 
GCGCAGAATG CCATCGAGGC TGCCGAGCGC GGGGACTTCT CAGAGGTGCG GCGGGTGCTG 
AAACTACTGG AGACCCCTTA CCACTGCGAG GCGGGGGCCG CCACAGACGC CGAGGCCACG 
GAAGCCGACG GGGCGGACGG CAGGCAGCGC TCCTACAGCA GTAAGCCCCC GCTCTGGGCA 
GCAGAACTGT GCGTGACATG ATCTTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113642.1
CDS	27..2036
Translation	MAVYRAALGASLAAARLLPLGRCSPSPAPRSTLSGAAMEPAPRWLAGLRFDNRALRALPVEAPPPGPEGAPSAPRPVPGACFTRVQPTPLRQPRLVALSEPALALLGLGAPPAREAEAEAALFFSGNALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTATGERWELQLKGAGPTPFSRQADGRKVLRSSIREFLCSEAMFHLGVPTTRAGACVTSESTVVRDVFYDGNPKYEQCTVVLRVASTFIRFGSFEIFKSADEHTGRAGPSVGRNDIRVQLLDYVISSFYPEIQAAHASDSVQRNAAFFREVTRRTARMVAEWQCVGFCHGVLNTDNMSILGLTIDYGPFGFLDRYDPDHVCNASDNTGRYAYSKQPEVCRWNLRKLAEALQPELPLELGEAILAEEFDAEFQRHYLQKMRRKLGLVQVELEEDGALVSKLLETMHLTGADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDPRQLSMMLMLAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQAYRARLDKDLEGAGDAAAWQAEHVRVMHANNPKYVLRNYIAQNAIEAAERGDFSEVRRVLKLLETPYHCEAGAATDAEATEADGADGRQRSYSSKPPLWAAELCVT*SS

Target ORF information:

RefSeq Version	NM_031454.2
Organism	Homo sapiens(human)
Definition	Homo sapiens selenoprotein O (SELENOO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031454.2

ORF Insert Sequence:

ATGGCCGTAT ACAGGGCAGC GCTCGGGGCT TCGCTCGCGG CTGCCCGACT CTTGCCCCTC 
GGTCGCTGTT CCCCGTCGCC GGCGCCCCGC TCTACGTTGT CGGGCGCCGC CATGGAGCCC 
GCGCCTCGCT GGCTGGCGGG GCTGCGCTTC GACAACCGCG CCCTGCGCGC CCTGCCCGTG 
GAGGCGCCGC CGCCCGGTCC CGAGGGCGCC CCGTCCGCGC CGCGGCCCGT GCCCGGGGCC 
TGCTTCACCC GCGTGCAGCC CACCCCGCTG CGGCAGCCGC GCCTCGTGGC GCTGTCAGAG 
CCCGCGCTGG CGTTGCTGGG CCTGGGCGCG CCGCCCGCGC GCGAGGCCGA GGCCGAGGCC 
GCGCTGTTCT TCAGCGGCAA CGCGCTCCTG CCGGGCGCCG AGCCCGCCGC GCACTGCTAC 
TGCGGCCACC AATTCGGCCA GTTCGCCGGG CAGCTGGGCG ACGGCGCCGC CATGTACCTG 
GGCGAGGTGT GCACGGCGAC CGGCGAGCGC TGGGAGCTGC AGCTCAAGGG CGCCGGGCCC 
ACGCCCTTCT CCAGACAGGC CGACGGTCGC AAGGTCCTAC GGTCAAGCAT CCGGGAGTTT 
CTATGCAGCG AAGCCATGTT CCACCTGGGA GTCCCCACCA CACGGGCCGG CGCCTGCGTC 
ACGTCCGAGT CCACGGTGGT GCGCGACGTG TTCTATGATG GTAATCCCAA ATATGAACAA 
TGCACGGTTG TGTTGCGTGT AGCTTCCACT TTCATAAGGT TTGGATCCTT TGAGATTTTT 
AAGTCTGCAG ATGAGCACAC AGGGCGTGCA GGCCCCAGCG TGGGGAGGAA CGACATTCGA 
GTGCAGCTGC TCGACTATGT CATCAGCTCC TTTTACCCCG AGATCCAGGC TGCTCATGCC 
AGCGACAGCG TGCAGAGAAA TGCTGCCTTC TTCCGGGAGG TGACGCGGCG CACGGCGCGG 
ATGGTGGCCG AGTGGCAGTG TGTGGGCTTC TGCCACGGCG TGCTCAACAC CGACAACATG 
AGCATCCTGG GGCTCACCAT CGACTACGGG CCCTTTGGCT TCCTGGACAG GTACGACCCC 
GACCACGTGT GCAATGCCTC CGACAACACC GGCCGCTACG CGTACAGCAA GCAGCCCGAG 
GTGTGCAGGT GGAACCTGCG GAAGCTGGCC GAGGCCCTGC AGCCGGAACT GCCCCTGGAG 
CTGGGGGAGG CCATCCTGGC CGAGGAGTTT GACGCCGAGT TCCAAAGGCA CTACCTGCAG 
AAGATGCGCA GGAAGCTGGG CCTCGTGCAG GTGGAGCTGG AGGAAGACGG GGCGCTGGTG 
TCCAAGCTCC TGGAGACCAT GCATCTGACC GGTGCCGACT TCACAAACAC CTTCTACTTG 
CTGAGCTCCT TCCCAGTGGA GCTAGAGTCG CCAGGCCTGG CGGAATTCCT GGCCAGGCTG 
ATGGAGCAGT GTGCCTCCCT GGAGGAGCTG AGGCTGGCCT TCCGGCCCCA GATGGATCCC 
CGGCAGCTAT CCATGATGCT GATGCTGGCG CAGTCAAACC CGCAGCTGTT CGCGCTTATG 
GGCACCCGGG CAGGCATCGC CAGGGAGCTG GAGCGTGTGG AGCAGCAGTC TCGGCTGGAG 
CAGCTGAGTG CGGCAGAGCT GCAGAGCAGG AACCAGGGCC ACTGGGCTGA CTGGCTACAG 
GCGTACAGAG CCCGGCTGGA CAAGGACCTG GAAGGCGCTG GGGACGCTGC CGCCTGGCAG 
GCTGAGCACG TGCGCGTGAT GCACGCCAAC AACCCGAAGT ACGTGCTGAG GAACTACATC 
GCGCAGAATG CCATCGAGGC TGCCGAGCGC GGGGACTTCT CAGAGGTGCG GCGGGTGCTG 
AAACTACTGG AGACCCCTTA CCACTGCGAG GCGGGGGCCG CCACAGACGC CGAGGCCACG 
GAAGCCGACG GGGCGGACGG CAGGCAGCGC TCCTACAGCA GTAAGCCCCC GCTCTGGGCA 
GCAGAACTGT GCGTGACATG ATCTTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Characterization of mammalian selenoprotein o: a redox-active mitochondrial protein.
PloS one9(4)e95518(2014)
Han SJ,Lee BC,Yim SH,Gladyshev VN,Lee SR

A novel protein kinase-like domain in a selenoprotein, widespread in the tree of life.
PloS one7(2)e32138(2012)
Dudkiewicz M,Szczepi?ska T,Grynberg M,Paw?owski K

A genome annotation-driven approach to cloning the human ORFeome.
Genome biology5(10)R84(2004)
Collins JE,Wright CL,Edwards CA,Davis MP,Grinham JA,Cole CG,Goward ME,Aguado B,Mallya M,Mokrab Y,Huckle EJ,Beare DM,Dunham I

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SELENOO cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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