H3C2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	H3C2
Entrez Gene ID	8358
Full Name	histone cluster 1 H3 family member b
Synonyms	H3/l,H3FL
General protein information	Preferred Names histone cluster 1 H3 family member b Names histone H3.1 H3 histone family, member L histone 1, H3b histone H3/l histone cluster 1, H3b
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p22.2
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015].
Disorder MIM:	602819

mRNA and Protein(s)

mRNA	Protein	Name
NM_003537.3	NP_003528.1	histone H3.1
NM_003537.4	NP_003528.1	histone H3.1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC101057734	XP_003950456.1
Mus musculus (house mouse)	Hist2h3b	NP_835587.1
Homo sapiens (human)	HIST1H3B	NP_003528.1
	LOC695891	XP_001084296.1
Drosophila melanogaster (fruit fly)	His3:CG31613	NP_724345.1
Xenopus tropicalis (tropical clawed frog)	LOC100493036	XP_002944659.1

Gene Ontology
Bibliography

Related articles in PubMed

Significance of H3K27M mutation with specific histomorphological features and associated molecular alterations in pediatric high-grade glial tumors.
Bozkurt SU, Dagcinar A, Tanrikulu B, Comunoglu N, Meydan BC, Ozek M, Oz B
Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery34(1)107-116(2018 Jan)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Spatial genomic heterogeneity in diffuse intrinsic pontine and midline high-grade glioma: implications for diagnostic biopsy and targeted therapeutics.
Hoffman LM, DeWire M, Ryall S, Buczkowicz P, Leach J, Miles L, Ramani A, Brudno M, Kumar SS, Drissi R, Dexheimer P, Salloum R, Chow L, Hummel T, Stevenson C, Lu QR, Jones B, Witte D, Aronow B, Hawkins CE, Fouladi M
Acta neuropathologica communications41(2016 Jan)

Histone H3F3A and HIST1H3B K27M mutations define two subgroups of diffuse intrinsic pontine gliomas with different prognosis and phenotypes.
Castel D, Philippe C, Calmon R, Le Dret L, Truffaux N, Boddaert N, Pag?s M, Taylor KR, Saulnier P, Lacroix L, Mackay A, Jones C, Sainte-Rose C, Blauwblomme T, Andreiuolo F, Puget S, Grill J, Varlet P, Debily MA
Acta neuropathologica130(6)815-27(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study showed that HIST1H3B (n = 3) across all primary, contiguous, and metastatic tumor sites in all Diffuse intrinsic pontine glioma.
Title: Spatial genomic heterogeneity in diffuse intrinsic pontine and midline high-grade glioma: implications for diagnostic biopsy and targeted therapeutics.

Study examined the relationship of K27M mutations in the distinct histone H3 variants (i.e. HIST1H3B and H3F3A) with specific pontine glioma biology
Title: Histone H3F3A and HIST1H3B K27M mutations define two subgroups of diffuse intrinsic pontine gliomas with different prognosis and phenotypes.

These data suggest that adult brainstem gliomas differ from adult supratentorial gliomas. In particular, histone genes (H3F3A (K27M) mutations are frequent in adult brainstem gliomas.
Title: Molecular analysis of diffuse intrinsic brainstem gliomas in adults.

These findings suggested that at metaphase Thr 3 phosphorylated histone H3 may also participate in kinetochore assembly to promote faithful chromosome segregation and serve as another recognition code for kinetochore proteins.
Title: Thr 3 phosphorylated histone H3 concentrates at centromeric chromatin at metaphase.

MSK1- and MSK2-mediated H3K27me3S28 phosphorylation serves as a mechanism to activate a subset of PcG target genes determined by the biological stimuli and thereby modulate the gene expression program determining cell fate.
Title: Polycomb group protein displacement and gene activation through MSK-dependent H3K27me3S28 phosphorylation.

The following H3C2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H3C2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13047	NM_003537.4 Latest version!	Homo sapiens H3 clustered histone 2 (H3C2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13047	NM_003537.3	Homo sapiens histone cluster 1 H3 family member b (HIST1H3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H3C2 ( NM_003537.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003528.1 Homo sapiens histone cluster 1 H3 family member b (HIST1H3B), mRNA.

Price & Availability↑

CloneID	OHu13047
Clone ID Related Accession (Same CDS sequence)	NM_003537.3 , NM_003537.4
Accession Version	NM_003537.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-03
Organism	Homo sapiens(human)
Product	histone H3.1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X00090.1. On Jun 12, 2002 this sequence version replaced NM_003537.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC069133.1, DB042078.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGTA CTAAACAGAC AGCTCGGAAA TCCACCGGCG GTAAAGCGCC ACGCAAGCAG 
CTGGCTACCA AGGCTGCTCG CAAGAGCGCG CCGGCTACCG GCGGCGTGAA AAAGCCTCAC 
CGTTACCGCC CGGGCACTGT GGCTCTGCGC GAGATCCGCC GCTACCAAAA GTCGACCGAG 
TTGCTGATTC GGAAGCTGCC GTTCCAGCGC CTGGTGCGAG AAATCGCCCA AGACTTCAAG 
ACCGATCTTC GCTTCCAGAG CTCTGCGGTG ATGGCGCTGC AGGAGGCTTG TGAGGCCTAC 
TTGGTAGGGC TCTTTGAGGA CACAAACCTT TGCGCCATCC ATGCTAAGCG AGTGACTATT 
ATGCCCAAAG ACATCCAGCT CGCTCGCCGC ATTCGCGGAG AAAGAGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003528.1
CDS	1..411
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_003537.3
Organism	Homo sapiens(human)
Definition	Homo sapiens histone cluster 1 H3 family member b (HIST1H3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003537.3

ORF Insert Sequence:

ATGGCTCGTA CTAAACAGAC AGCTCGGAAA TCCACCGGCG GTAAAGCGCC ACGCAAGCAG 
CTGGCTACCA AGGCTGCTCG CAAGAGCGCG CCGGCTACCG GCGGCGTGAA AAAGCCTCAC 
CGTTACCGCC CGGGCACTGT GGCTCTGCGC GAGATCCGCC GCTACCAAAA GTCGACCGAG 
TTGCTGATTC GGAAGCTGCC GTTCCAGCGC CTGGTGCGAG AAATCGCCCA AGACTTCAAG 
ACCGATCTTC GCTTCCAGAG CTCTGCGGTG ATGGCGCTGC AGGAGGCTTG TGAGGCCTAC 
TTGGTAGGGC TCTTTGAGGA CACAAACCTT TGCGCCATCC ATGCTAAGCG AGTGACTATT 
ATGCCCAAAG ACATCCAGCT CGCTCGCCGC ATTCGCGGAG AAAGAGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H3C2 ( NM_003537.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003528.1 Homo sapiens H3 clustered histone 2 (H3C2), mRNA.

Price & Availability↑

CloneID	OHu13047
Clone ID Related Accession (Same CDS sequence)	NM_003537.3 , NM_003537.4
Accession Version	NM_003537.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	histone H3.1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U91328.1. On Dec 3, 2019 this sequence version replaced NM_003537.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: DA003006.1, BP872375.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGTA CTAAACAGAC AGCTCGGAAA TCCACCGGCG GTAAAGCGCC ACGCAAGCAG 
CTGGCTACCA AGGCTGCTCG CAAGAGCGCG CCGGCTACCG GCGGCGTGAA AAAGCCTCAC 
CGTTACCGCC CGGGCACTGT GGCTCTGCGC GAGATCCGCC GCTACCAAAA GTCGACCGAG 
TTGCTGATTC GGAAGCTGCC GTTCCAGCGC CTGGTGCGAG AAATCGCCCA AGACTTCAAG 
ACCGATCTTC GCTTCCAGAG CTCTGCGGTG ATGGCGCTGC AGGAGGCTTG TGAGGCCTAC 
TTGGTAGGGC TCTTTGAGGA CACAAACCTT TGCGCCATCC ATGCTAAGCG AGTGACTATT 
ATGCCCAAAG ACATCCAGCT CGCTCGCCGC ATTCGCGGAG AAAGAGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003528.1
CDS	40..450
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_003537.4
Organism	Homo sapiens(human)
Definition	Homo sapiens H3 clustered histone 2 (H3C2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003537.4

ORF Insert Sequence:

ATGGCTCGTA CTAAACAGAC AGCTCGGAAA TCCACCGGCG GTAAAGCGCC ACGCAAGCAG 
CTGGCTACCA AGGCTGCTCG CAAGAGCGCG CCGGCTACCG GCGGCGTGAA AAAGCCTCAC 
CGTTACCGCC CGGGCACTGT GGCTCTGCGC GAGATCCGCC GCTACCAAAA GTCGACCGAG 
TTGCTGATTC GGAAGCTGCC GTTCCAGCGC CTGGTGCGAG AAATCGCCCA AGACTTCAAG 
ACCGATCTTC GCTTCCAGAG CTCTGCGGTG ATGGCGCTGC AGGAGGCTTG TGAGGCCTAC 
TTGGTAGGGC TCTTTGAGGA CACAAACCTT TGCGCCATCC ATGCTAAGCG AGTGACTATT 
ATGCCCAAAG ACATCCAGCT CGCTCGCCGC ATTCGCGGAG AAAGAGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Significance of H3K27M mutation with specific histomorphological features and associated molecular alterations in pediatric high-grade glial tumors.
Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery34(1)107-116(2018 Jan)
Bozkurt SU,Dagcinar A,Tanrikulu B,Comunoglu N,Meydan BC,Ozek M,Oz B

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Spatial genomic heterogeneity in diffuse intrinsic pontine and midline high-grade glioma: implications for diagnostic biopsy and targeted therapeutics.
Acta neuropathologica communications41(2016 Jan)
Hoffman LM,DeWire M,Ryall S,Buczkowicz P,Leach J,Miles L,Ramani A,Brudno M,Kumar SS,Drissi R,Dexheimer P,Salloum R,Chow L,Hummel T,Stevenson C,Lu QR,Jones B,Witte D,Aronow B,Hawkins CE,Fouladi M

Histone H3F3A and HIST1H3B K27M mutations define two subgroups of diffuse intrinsic pontine gliomas with different prognosis and phenotypes.
Acta neuropathologica130(6)815-27(2015 Dec)
Castel D,Philippe C,Calmon R,Le Dret L,Truffaux N,Boddaert N,Pag?s M,Taylor KR,Saulnier P,Lacroix L,Mackay A,Jones C,Sainte-Rose C,Blauwblomme T,Andreiuolo F,Puget S,Grill J,Varlet P,Debily MA

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H3C2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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