DNAL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNAL1
Entrez Gene ID	83544
Full Name	dynein axonemal light chain 1
Synonyms	C14orf168,CILD16,LC1
General protein information	Preferred Names dynein axonemal light chain 1 Names dynein light chain 1, axonemal
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q24.3
Summary	This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011].
Disorder MIM:	610062
Disorder Html:	Ciliary dyskinesia, primary, 16, 614017 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_031427.3	NP_113615.2	dynein light chain 1, axonemal isoform 1
NM_001201366.2	NP_001188295.1	dynein light chain 1, axonemal isoform 2
XM_011537204.1	XP_011535506.1	dynein light chain 1, axonemal isoform X1
XM_024449715.1	XP_024305483.1	dynein light chain 1, axonemal isoform X1
NM_031427.4	NP_113615.2	dynein light chain 1, axonemal isoform 1
XM_017021679.2	XP_016877168.1	dynein light chain 1, axonemal isoform X1
NM_001201366.1	NP_001188295.1	dynein light chain 1, axonemal isoform 2
XM_017021679.1	XP_016877168.1	dynein light chain 1, axonemal isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DNAL1	NP_113615.2
Rattus norvegicus (Norway rat)	Dnal1	NP_001102947.2
Xenopus tropicalis (tropical clawed frog)	dnal1	NP_001016312.1
Pan troglodytes (chimpanzee)	DNAL1	XP_001141594.1
Mus musculus (house mouse)	Dnal1	NP_083097.2
Gallus gallus (chicken)	DNAL1	NP_001186610.1
Danio rerio (zebrafish)	dnal1	NP_001003442.1
Drosophila melanogaster (fruit fly)	CG8800	NP_610483.1
	DNAL1	XP_001085992.1
Canis lupus familiaris (dog)	DNAL1	XP_853805.1
Bos taurus (cattle)	DNAL1	NP_001039892.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004416	XP_313700.4

Gene Ontology
Bibliography

Related articles in PubMed

Knockdown of MAP4 and DNAL1 produces a post-fusion and pre-nuclear translocation impairment in HIV-1 replication.
Gallo DE, Hope TJ
Virology422(1)13-21(2012 Jan)

Primary ciliary dyskinesia caused by homozygous mutation in DNAL1, encoding dynein light chain 1.
Mazor M, Alkrinawi S, Chalifa-Caspi V, Manor E, Sheffield VC, Aviram M, Parvari R
American journal of human genetics88(5)599-607(2011 May)

Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients.
Horv?th J, Fliegauf M, Olbrich H, Kispert A, King SM, Mitchison H, Zariwala MA, Knowles MR, Sudbrak R, Fekete G, Neesen J, Reinhardt R, Omran H
American journal of respiratory cell and molecular biology33(1)41-7(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DNAL1 and MAP4 may exert their functions in the HIV life cycle at reverse transcription, prior to nuclear translocation.
Title: Knockdown of MAP4 and DNAL1 produces a post-fusion and pre-nuclear translocation impairment in HIV-1 replication.

DNAL1 mutation could help to elucidate the interaction between the DNAL1 to dynein heavy chain and to tubulin.
Title: Primary ciliary dyskinesia caused by homozygous mutation in DNAL1, encoding dynein light chain 1.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

identification of the human (DNAL1) ortholog of the Chlamydomonas LC1-gene; protein interaction studies demonstrated binding of DNAL1 and DNAH5; DNAL1 considered a candidate for primary ciliary dyskinesia
Title: Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients.

The following DNAL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNAL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22137	NM_001201366.2 Latest version!	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22140	NM_031427.4 Latest version!	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22137	XM_017021679.2 Latest version!	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22137	XM_011537204.1 Latest version!	Homo sapiens dynein, axonemal, light chain 1 (DNAL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22137	XM_024449715.1 Latest version!	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu22140	NM_031427.3	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22137	NM_001201366.1	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22137	XM_017021679.1	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNAL1 ( NM_001201366.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188295.1 Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22137
Clone ID Related Accession (Same CDS sequence)	NM_001201366.2 , XM_011537204.1 , XM_024449715.1 , XM_017021679.2 , NM_001201366.1 , XM_017021679.1
Accession Version	NM_001201366.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	dynein light chain 1, axonemal isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI242197.1, AK315392.1, BJ996719.1, AC006146.2 and BQ267448.1. On Jun 1, 2019 this sequence version replaced NM_001201366.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.114738.1, SRR1803611.99619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188295.1
CDS	218..673
Translation	MDASLSMLANCEKLSLSTNCIEKIANLNGLKNLRILSLGRNNIKNLNGLEAVGDTLEELWISYNFIEKLKGIHIMKKLKILYMSNNLVKDWAEFVKLAELPCLEDLVFVGNPLEEKHSAENNWIEEATKRVPKLKKLDGTPVIKGDEEEDN

Target ORF information:

RefSeq Version	NM_001201366.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201366.2

ORF Insert Sequence:

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAL1 ( NM_001201366.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188295.1 Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22137
Clone ID Related Accession (Same CDS sequence)	NM_001201366.2 , XM_011537204.1 , XM_024449715.1 , XM_017021679.2 , NM_001201366.1 , XM_017021679.1
Accession Version	NM_001201366.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-20
Organism	Homo sapiens(human)
Product	dynein light chain 1, axonemal isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI242197.1, AK315392.1, BJ996719.1, AC006146.2 and BQ267448.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.114738.1, SRR1660809.80844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188295.1
CDS	329..784
Translation	MDASLSMLANCEKLSLSTNCIEKIANLNGLKNLRILSLGRNNIKNLNGLEAVGDTLEELWISYNFIEKLKGIHIMKKLKILYMSNNLVKDWAEFVKLAELPCLEDLVFVGNPLEEKHSAENNWIEEATKRVPKLKKLDGTPVIKGDEEEDN

Target ORF information:

RefSeq Version	NM_001201366.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201366.1

ORF Insert Sequence:

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAL1 ( NM_031427.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_113615.2 Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22140
Clone ID Related Accession (Same CDS sequence)	NM_031427.3 , NM_031427.4
Accession Version	NM_031427.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	dynein light chain 1, axonemal isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI242197.1, AF542071.1, BJ996719.1, AC006146.2 and BQ267448.1. This sequence is a reference standard in the RefSeqGene project. On Jan 15, 2011 this sequence version replaced NM_031427.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.246688.1, SRR1660803.220721.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAAG CAACAACAAT CAAAGAAGCC TTAGCGAGAT GGGAAGAGAA AACTGGCCAG 
AGGCCATCTG AAGCCAAAGA GATAAAACTT TATGCCCAGA TTCCCCCTAT AGAGAAGATG 
GATGCATCCT TGTCCATGCT TGCTAATTGC GAGAAGCTTT CACTGTCTAC AAACTGCATT 
GAAAAAATTG CCAACCTGAA TGGCTTAAAA AACTTGAGGA TATTATCTTT AGGAAGAAAC 
AACATAAAGA ACTTAAATGG ACTGGAGGCA GTAGGGGACA CATTAGAAGA ACTGTGGATC 
TCCTACAATT TTATTGAGAA GTTGAAAGGG ATCCACATAA TGAAGAAATT GAAGATTCTC 
TACATGTCTA ATAACCTGGT AAAAGACTGG GCTGAGTTTG TGAAGCTGGC AGAACTGCCA 
TGCCTCGAAG ACCTGGTGTT TGTAGGCAAT CCCTTGGAAG AGAAACATTC TGCTGAGAAT 
AACTGGATTG AAGAAGCAAC CAAGAGAGTG CCCAAACTGA AAAAGCTGGA TGGTACTCCA 
GTAATTAAAG GGGATGAGGA AGAAGACAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113615.2
CDS	166..738
Translation	MAKATTIKEALARWEEKTGQRPSEAKEIKLYAQIPPIEKMDASLSMLANCEKLSLSTNCIEKIANLNGLKNLRILSLGRNNIKNLNGLEAVGDTLEELWISYNFIEKLKGIHIMKKLKILYMSNNLVKDWAEFVKLAELPCLEDLVFVGNPLEEKHSAENNWIEEATKRVPKLKKLDGTPVIKGDEEEDN

Target ORF information:

RefSeq Version	NM_031427.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031427.3

ORF Insert Sequence:

ATGGCGAAAG CAACAACAAT CAAAGAAGCC TTAGCGAGAT GGGAAGAGAA AACTGGCCAG 
AGGCCATCTG AAGCCAAAGA GATAAAACTT TATGCCCAGA TTCCCCCTAT AGAGAAGATG 
GATGCATCCT TGTCCATGCT TGCTAATTGC GAGAAGCTTT CACTGTCTAC AAACTGCATT 
GAAAAAATTG CCAACCTGAA TGGCTTAAAA AACTTGAGGA TATTATCTTT AGGAAGAAAC 
AACATAAAGA ACTTAAATGG ACTGGAGGCA GTAGGGGACA CATTAGAAGA ACTGTGGATC 
TCCTACAATT TTATTGAGAA GTTGAAAGGG ATCCACATAA TGAAGAAATT GAAGATTCTC 
TACATGTCTA ATAACCTGGT AAAAGACTGG GCTGAGTTTG TGAAGCTGGC AGAACTGCCA 
TGCCTCGAAG ACCTGGTGTT TGTAGGCAAT CCCTTGGAAG AGAAACATTC TGCTGAGAAT 
AACTGGATTG AAGAAGCAAC CAAGAGAGTG CCCAAACTGA AAAAGCTGGA TGGTACTCCA 
GTAATTAAAG GGGATGAGGA AGAAGACAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAL1 ( NM_031427.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_113615.2 Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22140
Clone ID Related Accession (Same CDS sequence)	NM_031427.3 , NM_031427.4
Accession Version	NM_031427.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	dynein light chain 1, axonemal isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI242197.1, AF542071.1, BJ996719.1, AC006146.2 and BQ267448.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_031427.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.663198.1, SRR3476690.1014941.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000553645.7/ ENSP00000452037.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGAAAG CAACAACAAT CAAAGAAGCC TTAGCGAGAT GGGAAGAGAA AACTGGCCAG 
AGGCCATCTG AAGCCAAAGA GATAAAACTT TATGCCCAGA TTCCCCCTAT AGAGAAGATG 
GATGCATCCT TGTCCATGCT TGCTAATTGC GAGAAGCTTT CACTGTCTAC AAACTGCATT 
GAAAAAATTG CCAACCTGAA TGGCTTAAAA AACTTGAGGA TATTATCTTT AGGAAGAAAC 
AACATAAAGA ACTTAAATGG ACTGGAGGCA GTAGGGGACA CATTAGAAGA ACTGTGGATC 
TCCTACAATT TTATTGAGAA GTTGAAAGGG ATCCACATAA TGAAGAAATT GAAGATTCTC 
TACATGTCTA ATAACCTGGT AAAAGACTGG GCTGAGTTTG TGAAGCTGGC AGAACTGCCA 
TGCCTCGAAG ACCTGGTGTT TGTAGGCAAT CCCTTGGAAG AGAAACATTC TGCTGAGAAT 
AACTGGATTG AAGAAGCAAC CAAGAGAGTG CCCAAACTGA AAAAGCTGGA TGGTACTCCA 
GTAATTAAAG GGGATGAGGA AGAAGACAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113615.2
CDS	55..627
Translation	MAKATTIKEALARWEEKTGQRPSEAKEIKLYAQIPPIEKMDASLSMLANCEKLSLSTNCIEKIANLNGLKNLRILSLGRNNIKNLNGLEAVGDTLEELWISYNFIEKLKGIHIMKKLKILYMSNNLVKDWAEFVKLAELPCLEDLVFVGNPLEEKHSAENNWIEEATKRVPKLKKLDGTPVIKGDEEEDN

Target ORF information:

RefSeq Version	NM_031427.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031427.4

ORF Insert Sequence:

ATGGCGAAAG CAACAACAAT CAAAGAAGCC TTAGCGAGAT GGGAAGAGAA AACTGGCCAG 
AGGCCATCTG AAGCCAAAGA GATAAAACTT TATGCCCAGA TTCCCCCTAT AGAGAAGATG 
GATGCATCCT TGTCCATGCT TGCTAATTGC GAGAAGCTTT CACTGTCTAC AAACTGCATT 
GAAAAAATTG CCAACCTGAA TGGCTTAAAA AACTTGAGGA TATTATCTTT AGGAAGAAAC 
AACATAAAGA ACTTAAATGG ACTGGAGGCA GTAGGGGACA CATTAGAAGA ACTGTGGATC 
TCCTACAATT TTATTGAGAA GTTGAAAGGG ATCCACATAA TGAAGAAATT GAAGATTCTC 
TACATGTCTA ATAACCTGGT AAAAGACTGG GCTGAGTTTG TGAAGCTGGC AGAACTGCCA 
TGCCTCGAAG ACCTGGTGTT TGTAGGCAAT CCCTTGGAAG AGAAACATTC TGCTGAGAAT 
AACTGGATTG AAGAAGCAAC CAAGAGAGTG CCCAAACTGA AAAAGCTGGA TGGTACTCCA 
GTAATTAAAG GGGATGAGGA AGAAGACAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAL1 ( XM_011537204.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535506.1 Homo sapiens dynein, axonemal, light chain 1 (DNAL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22137
Clone ID Related Accession (Same CDS sequence)	NM_001201366.2 , XM_011537204.1 , XM_024449715.1 , XM_017021679.2 , NM_001201366.1 , XM_017021679.1
Accession Version	XM_011537204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	dynein light chain 1, axonemal isoform X1
Comment

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535506.1
Translation	MDASLSMLANCEKLSLSTNCIEKIANLNGLKNLRILSLGRNNIKNLNGLEAVGDTLEELWISYNFIEKLKGIHIMKKLKILYMSNNLVKDWAEFVKLAELPCLEDLVFVGNPLEEKHSAENNWIEEATKRVPKLKKLDGTPVIKGDEEEDN

Target ORF information:

RefSeq Version	XM_011537204.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens dynein, axonemal, light chain 1 (DNAL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537204.1

ORF Insert Sequence:

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAL1 ( XM_024449715.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305483.1 Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu22137
Clone ID Related Accession (Same CDS sequence)	NM_001201366.2 , XM_011537204.1 , XM_024449715.1 , XM_017021679.2 , NM_001201366.1 , XM_017021679.1
Accession Version	XM_024449715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dynein light chain 1, axonemal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305483.1
CDS	246..701
Translation	MDASLSMLANCEKLSLSTNCIEKIANLNGLKNLRILSLGRNNIKNLNGLEAVGDTLEELWISYNFIEKLKGIHIMKKLKILYMSNNLVKDWAEFVKLAELPCLEDLVFVGNPLEEKHSAENNWIEEATKRVPKLKKLDGTPVIKGDEEEDN

Target ORF information:

RefSeq Version	XM_024449715.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449715.1

ORF Insert Sequence:

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAL1 ( XM_017021679.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877168.1 Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22137
Clone ID Related Accession (Same CDS sequence)	NM_001201366.2 , XM_011537204.1 , XM_024449715.1 , XM_017021679.2 , NM_001201366.1 , XM_017021679.1
Accession Version	XM_017021679.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dynein light chain 1, axonemal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877168.1
CDS	317..772
Translation	MDASLSMLANCEKLSLSTNCIEKIANLNGLKNLRILSLGRNNIKNLNGLEAVGDTLEELWISYNFIEKLKGIHIMKKLKILYMSNNLVKDWAEFVKLAELPCLEDLVFVGNPLEEKHSAENNWIEEATKRVPKLKKLDGTPVIKGDEEEDN

Target ORF information:

RefSeq Version	XM_017021679.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021679.2

ORF Insert Sequence:

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAL1 ( XM_017021679.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877168.1 Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22137
Clone ID Related Accession (Same CDS sequence)	NM_001201366.2 , XM_011537204.1 , XM_024449715.1 , XM_017021679.2 , NM_001201366.1 , XM_017021679.1
Accession Version	XM_017021679.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	dynein light chain 1, axonemal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877168.1
CDS	317..772
Translation	MDASLSMLANCEKLSLSTNCIEKIANLNGLKNLRILSLGRNNIKNLNGLEAVGDTLEELWISYNFIEKLKGIHIMKKLKILYMSNNLVKDWAEFVKLAELPCLEDLVFVGNPLEEKHSAENNWIEEATKRVPKLKKLDGTPVIKGDEEEDN

Target ORF information:

RefSeq Version	XM_017021679.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynein axonemal light chain 1 (DNAL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021679.1

ORF Insert Sequence:

ATGGATGCAT CCTTGTCCAT GCTTGCTAAT TGCGAGAAGC TTTCACTGTC TACAAACTGC 
ATTGAAAAAA TTGCCAACCT GAATGGCTTA AAAAACTTGA GGATATTATC TTTAGGAAGA 
AACAACATAA AGAACTTAAA TGGACTGGAG GCAGTAGGGG ACACATTAGA AGAACTGTGG 
ATCTCCTACA ATTTTATTGA GAAGTTGAAA GGGATCCACA TAATGAAGAA ATTGAAGATT 
CTCTACATGT CTAATAACCT GGTAAAAGAC TGGGCTGAGT TTGTGAAGCT GGCAGAACTG 
CCATGCCTCG AAGACCTGGT GTTTGTAGGC AATCCCTTGG AAGAGAAACA TTCTGCTGAG 
AATAACTGGA TTGAAGAAGC AACCAAGAGA GTGCCCAAAC TGAAAAAGCT GGATGGTACT 
CCAGTAATTA AAGGGGATGA GGAAGAAGAC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knockdown of MAP4 and DNAL1 produces a post-fusion and pre-nuclear translocation impairment in HIV-1 replication.
Virology422(1)13-21(2012 Jan)
Gallo DE,Hope TJ

Primary ciliary dyskinesia caused by homozygous mutation in DNAL1, encoding dynein light chain 1.
American journal of human genetics88(5)599-607(2011 May)
Mazor M,Alkrinawi S,Chalifa-Caspi V,Manor E,Sheffield VC,Aviram M,Parvari R

Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients.
American journal of respiratory cell and molecular biology33(1)41-7(2005 Jul)
Horv?th J,Fliegauf M,Olbrich H,Kispert A,King SM,Mitchison H,Zariwala MA,Knowles MR,Sudbrak R,Fekete G,Neesen J,Reinhardt R,Omran H

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DNAL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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